Aggregation-induced electrochemiluminescence aptasensor for ultrasensitive detection of acetamiprid based on polymer dots as emitters

IF 10.5 1区生物学 Q1 BIOPHYSICS

Biosensors and Bioelectronics Pub Date : 2025-09-25 DOI:10.1016/j.bios.2025.118031

Yuanyuan Jin, Yerong Zhao, Haibing Zhu, Shijie He, Hang Gao, Lingling Xu, Zhanjun Yang

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引用次数: 0

Abstract

Acetamiprid (ACE) poses significant threats to the environment and human health. Thus, the development of sensitive ACE detection is crucial for safeguarding human health. Herein, we developed an aggregation-induced electrochemiluminescence (AIECL) aptasensor for ACE detection, in which polymer dots (Pdots) are used as luminescent probe. By virtue of the electrochemiluminescence-resonance energy transfer (ECL-RET) mechanism, ECL signal of Pdots is quenched by black hole quencher (BHQ) linked to ACE aptamer, thereby turning “off” the signal of sensor. Upon the existence of ACE, BHQ group releases from the Pdots and escapes from the surface of sensor, consequently enabling the restoration of ECL signal of Pdots. Such sensor exhibits excellent analysis performance with a lower detection limit of 9.1 aM, over conventional ECL analysis for ACE. Significantly, the sensor is applied to lettuce sample with good recovery rate. This work provides an effective method for ACE detection, and validates its application potential in food safety supervision.

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基于聚合物点的聚类诱导电化学发光对啶虫脒超灵敏检测传感器。

Acetamiprid （ACE）对环境和人类健康构成重大威胁。因此，开发灵敏的ACE检测方法对于保障人类健康至关重要。在此，我们开发了一种用于ACE检测的聚集诱导电化学发光（AIECL）适体传感器，其中聚合物点（Pdots）作为发光探针。利用电化学发光-共振能量传递（ECL- ret）机制，Pdots的ECL信号被与ACE适配体相连的黑洞猝灭器（BHQ）猝灭，从而“关闭”传感器信号。ACE存在后，BHQ基团从Pdots中释放并从传感器表面逸出，从而使Pdots的ECL信号得以恢复。与传统的ECL分析相比，该传感器具有较低的检测限9.1 aM的优异分析性能。该传感器应用于莴苣样品中，具有良好的回收率。本研究为ACE检测提供了有效的方法，验证了其在食品安全监管中的应用潜力。

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来源期刊

Biosensors and Bioelectronics 工程技术-电化学

CiteScore

20.80

自引率

7.10%

发文量

1006

审稿时长

29 days

期刊介绍： Biosensors & Bioelectronics, along with its open access companion journal Biosensors & Bioelectronics: X, is the leading international publication in the field of biosensors and bioelectronics. It covers research, design, development, and application of biosensors, which are analytical devices incorporating biological materials with physicochemical transducers. These devices, including sensors, DNA chips, electronic noses, and lab-on-a-chip, produce digital signals proportional to specific analytes. Examples include immunosensors and enzyme-based biosensors, applied in various fields such as medicine, environmental monitoring, and food industry. The journal also focuses on molecular and supramolecular structures for enhancing device performance.