Ternary hybrids accelerated photoelectrochemical biosensor for sensitive detection of Alzheimer's disease biomarker

IF 10.5 1区生物学 Q1 BIOPHYSICS

Biosensors and Bioelectronics Pub Date : 2025-09-26 DOI:10.1016/j.bios.2025.118033

Junpeng Gao , Kang Du , Yuyang Xue , Pan Xu , Siqi Ding , Qiumei Feng , Xiaokai Wang

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引用次数: 0

Abstract

Amyloid β oligomer (AβO) is not only a crucial biomarker but also a potential therapeutic target for Alzheimer's disease (AD). Here, a type of ternary photoactive hybrids (CdS/ZnS/Bi₂Se₃) was used as a substrate material to construct a photoelectrochemical (PEC) biosensor for dual-quenching detection of AβO. Due to the cooperative band gap excitations and multi-interfacial charge transfer of the three components, CdS/ZnS/Bi₂Se₃ ternary hybrids possessed self-enhanced photocurrent, which significantly boosted PEC responses. The broad-spectrum absorption and peroxidase-mimicking catalytic property of PtCu nanoflowers (NFs) achieved the synergistic dual-quenching effect, which displayed a more sensitive response than common single-quenching PEC biosensors. Impressively, an efficient dynamic signal amplification strategy was established by DNA cascade reaction-activated DNA walker, improving the walk speed and high reaction efficiency. As a result, the constructed biosensor realized the detection of AβO ranging from 20 fM to 10 nM with a detection limit of 4.4 fM. In comparison to previously reported methods, the biosensor enabled the reliable detection of AβO in blood specimens from AD patients and healthy volunteers, demonstrating high sensitivity, specificity, stability, and reproducibility. By simply replacing the aptamer sequences, this strategy could be applied for other biomarker detection with a high potential to inspire innovative sensing approaches, offering some useful help for biomedical research and disease diagnosis.

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用于阿尔茨海默病生物标志物灵敏检测的三元杂化加速光电化学生物传感器

β淀粉样蛋白寡聚物（a β o）不仅是一种重要的生物标志物，而且是阿尔茨海默病（AD）的潜在治疗靶点。本文以一种三元光活性杂化物（CdS/ZnS/Bi2Se3）为衬底材料，构建了用于双猝灭检测a β o的光电化学（PEC）生物传感器。CdS/ZnS/Bi2Se3三元杂化物由于协同带隙激发和多界面电荷转移，具有自增强的光电流，显著提高了PEC响应。PtCu纳米花（NFs）的广谱吸收和模拟过氧化物酶的催化特性实现了协同双猝灭效应，比普通的单猝灭PEC生物传感器表现出更灵敏的响应。令人印象深刻的是，DNA级联反应激活的DNA步行者建立了一种高效的动态信号放大策略，提高了步行速度和高反应效率。结果表明，构建的生物传感器可实现对a β o在20 ~ 10 nM范围内的检测，检测限为4.4 fM。与先前报道的方法相比，该生物传感器能够可靠地检测AD患者和健康志愿者血液标本中的AβO，具有高灵敏度、特异性、稳定性和可重复性。通过简单地替换适体序列，该策略可以应用于其他生物标志物的检测，具有激发创新传感方法的高潜力，为生物医学研究和疾病诊断提供一些有用的帮助。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Biosensors and Bioelectronics 工程技术-电化学

CiteScore

20.80

自引率

7.10%

发文量

1006

审稿时长

29 days

期刊介绍： Biosensors & Bioelectronics, along with its open access companion journal Biosensors & Bioelectronics: X, is the leading international publication in the field of biosensors and bioelectronics. It covers research, design, development, and application of biosensors, which are analytical devices incorporating biological materials with physicochemical transducers. These devices, including sensors, DNA chips, electronic noses, and lab-on-a-chip, produce digital signals proportional to specific analytes. Examples include immunosensors and enzyme-based biosensors, applied in various fields such as medicine, environmental monitoring, and food industry. The journal also focuses on molecular and supramolecular structures for enhancing device performance.