Inner filter effect regulated upconversion sensing platform for non-invasive detection of two gastric cancer relevant substances

IF 10.5 1区 生物学 Q1 BIOPHYSICS
Guirong Cheng , Xinyu Shao , Peiyuan Lin, Jingwen Zhang, Lixia Lu
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引用次数: 0

Abstract

Gastric cancer is the fifth most common cancer in the world. The concentrations of D-Pro and D-Ala in the saliva of patients with early gastric cancer were significantly higher than those of healthy people. Urease has also been found to be closely associated with gastric cancer. Therefore, monitoring the concentration of D-amino acids and urease is of great significance for the diagnosis and treatment of gastric cancer. In this study, multi-peak emission UCNPs (ES-UCNPs) was synthesized based on emulsion self-assembly strategy. Polyethylenimine (PEI) modified copper-based Prussian blue analogues (PEI-CuFc) exhibit enhanced the stability and peroxidase-like catalytic activity. And we developed a colorimetric-luminescent dual-mode sensing platform for two gastric cancer relevant substances by utilizing PEI-CuFc nanozymes to regulate the luminescence spectrum through IFE mechanism. Notably, besides serving as the foundation for emulsion self-assembly, sodium dodecyl sulfate (SDS) effectively improved the catalytic activity of PEI-CuFc, significantly amplifying the detectable signal and thereby enhancing assay sensitivity. To our knowledge, this study presents the first report of simultaneous detection of these two gastric cancer relevant substances in a single assay. The detection limits fall within the clinically relevant concentration range of early gastric cancer biomarkers, offering a promising approach for non-invasive early diagnosis of gastric cancer.
内滤效应调节上转换传感平台无创检测两种胃癌相关物质。
胃癌是世界上第五大常见癌症。早期胃癌患者唾液中D-Pro和D-Ala浓度明显高于健康人。脲酶也被发现与胃癌密切相关。因此,监测d -氨基酸和脲酶的浓度对胃癌的诊断和治疗具有重要意义。本研究基于乳液自组装策略合成了多峰发射UCNPs (ES-UCNPs)。聚乙烯亚胺(PEI)修饰的铜基普鲁士蓝类似物(PEI- cufc)表现出增强的稳定性和类似过氧化物酶的催化活性。我们利用PEI-CuFc纳米酶通过IFE机制调节两种胃癌相关物质的发光光谱,建立了比色-发光双模传感平台。值得注意的是,十二烷基硫酸钠(SDS)除了作为乳液自组装的基础外,还有效地提高了PEI-CuFc的催化活性,显著放大了检测信号,从而提高了检测灵敏度。据我们所知,本研究首次报道了在一次检测中同时检测这两种胃癌相关物质。检测限均在早期胃癌生物标志物临床相关浓度范围内,为胃癌无创早期诊断提供了一种有前景的方法。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
Biosensors and Bioelectronics
Biosensors and Bioelectronics 工程技术-电化学
CiteScore
20.80
自引率
7.10%
发文量
1006
审稿时长
29 days
期刊介绍: Biosensors & Bioelectronics, along with its open access companion journal Biosensors & Bioelectronics: X, is the leading international publication in the field of biosensors and bioelectronics. It covers research, design, development, and application of biosensors, which are analytical devices incorporating biological materials with physicochemical transducers. These devices, including sensors, DNA chips, electronic noses, and lab-on-a-chip, produce digital signals proportional to specific analytes. Examples include immunosensors and enzyme-based biosensors, applied in various fields such as medicine, environmental monitoring, and food industry. The journal also focuses on molecular and supramolecular structures for enhancing device performance.
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