基于多功能比色荧光原位银纳米簇/石墨烯纳米杂合酶的超高效无标记细胞死亡检测及其在环境生物样品和细胞成像中的应用

IF 10.5 1区 生物学 Q1 BIOPHYSICS
Fatemeh Molaabasi , Nasim Mohseni , Behnam Hajipour-Verdom , Arash Agheli , Ali Akbar Moosavi-Movahedi , Reza Mahdavian , Afsaneh Roshanfekr , Mojtaba Shamsipur , Hossein Naderi-Manesh , Reza Gholami , Vahid Yousefi , Amirhosein Kefayat , Ramin Sarrami‐Forooshani
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引用次数: 0

摘要

本研究设计了一种基于原位血红蛋白稳定的银纳米簇/石墨烯纳米杂合酶(Hb-AgNCs/石墨烯NHZs)的高效无标记多功能探针,并将其用于双峰肉眼比色法和荧光法检测涉及铁凋亡、铜凋亡和细胞凋亡的细胞死亡生物标志物。发达nhz的有趣特征是“开启”的Fe2+、Cu2+和Cyt c和“关闭”的半胱氨酸(Cys)和谷胱甘肽(GSH)的比色形态,以及它从Fe3+中形成Fe2+的能力。在适当的线性范围内,分析物的存在导致比色检测限为10.8-191 nM,荧光检测限为0.25至121 nM,快速时间为5-20 min。设计的nhz成功地用于HT29人结肠癌的荧光成像,并在2 μL的细胞裂解物、环境和生物样品中进行了定量验证。与传统的半定量Western blot和其他细胞死亡检测方法相比,该探针具有成本低、制备简单、灵敏度高、多功能、双模态等特点,无需使用任何靶向剂即可形成物种和定量,适合在极短时间内进行细胞死亡过程的双模态跟踪和药物评价。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Ultra-efficient label-free cell death detection based on multifunctional colorimetric fluorescent in-situ silver nanocluster/graphene nanohybridzyme and its application in environmental biological samples and cellular imaging
In this study an efficient label-free multifunctional probe based on in situ hemoglobin-stabilized silver nanocluster/graphene nanohybridzymes (Hb-AgNCs/graphene NHZs) was designed and employed for dual-modal naked-eye colorimetric and fluorometric detection of cell death biomarkers involved in ferroptosis, cuproptosis, and apoptosis. The interesting feature of developed NHZs is the colorimetric speciation of “turn-on” Fe2+, Cu2+, and Cyt c and “turn-off” cysteine (Cys) and glutathione (GSH), and its speciation ability of Fe2+ from Fe3+as well. The presence of analytes causes a colorimetric limit of detection of 10.8–191 nM and fluorometric detection limit from 0.25 to 121 nM in the appropriate linear ranges, with fast times ranging 5–20 min. The designed NHZs were successfully employed for fluorescence imaging of HT29 human colon cancer and also quantitatively validated in just 2 μL of cell lysates, environmental, and biological samples. Compared to the semi-quantitative conventional Western blot and other cell death assays, the novelty of presented probe is low cost, simple preparation, high sensitivity, multifunctionallity, and dual-modality with the speciation and quantitative ability without using any targeting agent, which makes it appropriate for dual-modal tracking cell death processes and drug evaluation in a very short time.
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来源期刊
Biosensors and Bioelectronics
Biosensors and Bioelectronics 工程技术-电化学
CiteScore
20.80
自引率
7.10%
发文量
1006
审稿时长
29 days
期刊介绍: Biosensors & Bioelectronics, along with its open access companion journal Biosensors & Bioelectronics: X, is the leading international publication in the field of biosensors and bioelectronics. It covers research, design, development, and application of biosensors, which are analytical devices incorporating biological materials with physicochemical transducers. These devices, including sensors, DNA chips, electronic noses, and lab-on-a-chip, produce digital signals proportional to specific analytes. Examples include immunosensors and enzyme-based biosensors, applied in various fields such as medicine, environmental monitoring, and food industry. The journal also focuses on molecular and supramolecular structures for enhancing device performance.
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