{"title":"基于多功能比色荧光原位银纳米簇/石墨烯纳米杂合酶的超高效无标记细胞死亡检测及其在环境生物样品和细胞成像中的应用","authors":"Fatemeh Molaabasi , Nasim Mohseni , Behnam Hajipour-Verdom , Arash Agheli , Ali Akbar Moosavi-Movahedi , Reza Mahdavian , Afsaneh Roshanfekr , Mojtaba Shamsipur , Hossein Naderi-Manesh , Reza Gholami , Vahid Yousefi , Amirhosein Kefayat , Ramin Sarrami‐Forooshani","doi":"10.1016/j.bios.2025.118020","DOIUrl":null,"url":null,"abstract":"<div><div>In this study an efficient label-free multifunctional probe based on <em>in situ</em> hemoglobin-stabilized silver nanocluster/graphene nanohybridzymes (Hb-AgNCs/graphene NHZs) was designed and employed for dual-modal naked-eye colorimetric and fluorometric detection of cell death biomarkers involved in ferroptosis, cuproptosis, and apoptosis. The interesting feature of developed NHZs is the colorimetric speciation of “turn-on” Fe<sup>2+</sup>, Cu<sup>2+</sup>, and Cyt <em>c</em> and “turn-off” cysteine (Cys) and glutathione (GSH), and its speciation ability of Fe<sup>2+</sup> from Fe<sup>3+</sup>as well. The presence of analytes causes a colorimetric limit of detection of 10.8–191 nM and fluorometric detection limit from 0.25 to 121 nM in the appropriate linear ranges, with fast times ranging 5–20 min. The designed NHZs were successfully employed for fluorescence imaging of HT29 human colon cancer and also quantitatively validated in just 2 μL of cell lysates, environmental, and biological samples. Compared to the semi-quantitative conventional Western blot and other cell death assays, the novelty of presented probe is low cost, simple preparation, high sensitivity, multifunctionallity, and dual-modality with the speciation and quantitative ability without using any targeting agent, which makes it appropriate for dual-modal tracking cell death processes and drug evaluation in a very short time.</div></div>","PeriodicalId":259,"journal":{"name":"Biosensors and Bioelectronics","volume":"291 ","pages":"Article 118020"},"PeriodicalIF":10.5000,"publicationDate":"2025-09-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Ultra-efficient label-free cell death detection based on multifunctional colorimetric fluorescent in-situ silver nanocluster/graphene nanohybridzyme and its application in environmental biological samples and cellular imaging\",\"authors\":\"Fatemeh Molaabasi , Nasim Mohseni , Behnam Hajipour-Verdom , Arash Agheli , Ali Akbar Moosavi-Movahedi , Reza Mahdavian , Afsaneh Roshanfekr , Mojtaba Shamsipur , Hossein Naderi-Manesh , Reza Gholami , Vahid Yousefi , Amirhosein Kefayat , Ramin Sarrami‐Forooshani\",\"doi\":\"10.1016/j.bios.2025.118020\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><div>In this study an efficient label-free multifunctional probe based on <em>in situ</em> hemoglobin-stabilized silver nanocluster/graphene nanohybridzymes (Hb-AgNCs/graphene NHZs) was designed and employed for dual-modal naked-eye colorimetric and fluorometric detection of cell death biomarkers involved in ferroptosis, cuproptosis, and apoptosis. The interesting feature of developed NHZs is the colorimetric speciation of “turn-on” Fe<sup>2+</sup>, Cu<sup>2+</sup>, and Cyt <em>c</em> and “turn-off” cysteine (Cys) and glutathione (GSH), and its speciation ability of Fe<sup>2+</sup> from Fe<sup>3+</sup>as well. The presence of analytes causes a colorimetric limit of detection of 10.8–191 nM and fluorometric detection limit from 0.25 to 121 nM in the appropriate linear ranges, with fast times ranging 5–20 min. The designed NHZs were successfully employed for fluorescence imaging of HT29 human colon cancer and also quantitatively validated in just 2 μL of cell lysates, environmental, and biological samples. Compared to the semi-quantitative conventional Western blot and other cell death assays, the novelty of presented probe is low cost, simple preparation, high sensitivity, multifunctionallity, and dual-modality with the speciation and quantitative ability without using any targeting agent, which makes it appropriate for dual-modal tracking cell death processes and drug evaluation in a very short time.</div></div>\",\"PeriodicalId\":259,\"journal\":{\"name\":\"Biosensors and Bioelectronics\",\"volume\":\"291 \",\"pages\":\"Article 118020\"},\"PeriodicalIF\":10.5000,\"publicationDate\":\"2025-09-23\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Biosensors and Bioelectronics\",\"FirstCategoryId\":\"1\",\"ListUrlMain\":\"https://www.sciencedirect.com/science/article/pii/S0956566325008966\",\"RegionNum\":1,\"RegionCategory\":\"生物学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q1\",\"JCRName\":\"BIOPHYSICS\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Biosensors and Bioelectronics","FirstCategoryId":"1","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S0956566325008966","RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"BIOPHYSICS","Score":null,"Total":0}
Ultra-efficient label-free cell death detection based on multifunctional colorimetric fluorescent in-situ silver nanocluster/graphene nanohybridzyme and its application in environmental biological samples and cellular imaging
In this study an efficient label-free multifunctional probe based on in situ hemoglobin-stabilized silver nanocluster/graphene nanohybridzymes (Hb-AgNCs/graphene NHZs) was designed and employed for dual-modal naked-eye colorimetric and fluorometric detection of cell death biomarkers involved in ferroptosis, cuproptosis, and apoptosis. The interesting feature of developed NHZs is the colorimetric speciation of “turn-on” Fe2+, Cu2+, and Cyt c and “turn-off” cysteine (Cys) and glutathione (GSH), and its speciation ability of Fe2+ from Fe3+as well. The presence of analytes causes a colorimetric limit of detection of 10.8–191 nM and fluorometric detection limit from 0.25 to 121 nM in the appropriate linear ranges, with fast times ranging 5–20 min. The designed NHZs were successfully employed for fluorescence imaging of HT29 human colon cancer and also quantitatively validated in just 2 μL of cell lysates, environmental, and biological samples. Compared to the semi-quantitative conventional Western blot and other cell death assays, the novelty of presented probe is low cost, simple preparation, high sensitivity, multifunctionallity, and dual-modality with the speciation and quantitative ability without using any targeting agent, which makes it appropriate for dual-modal tracking cell death processes and drug evaluation in a very short time.
期刊介绍:
Biosensors & Bioelectronics, along with its open access companion journal Biosensors & Bioelectronics: X, is the leading international publication in the field of biosensors and bioelectronics. It covers research, design, development, and application of biosensors, which are analytical devices incorporating biological materials with physicochemical transducers. These devices, including sensors, DNA chips, electronic noses, and lab-on-a-chip, produce digital signals proportional to specific analytes. Examples include immunosensors and enzyme-based biosensors, applied in various fields such as medicine, environmental monitoring, and food industry. The journal also focuses on molecular and supramolecular structures for enhancing device performance.