包覆铂(II)配合物的纳米金属有机框架与自催化聚集诱导的电化学发光用于超灵敏的miRNA-21生物传感

IF 10.5 1区 生物学 Q1 BIOPHYSICS
Wanzhen Chen , Ziqi Lian , Rongkai Ye , Jiaju Mo , Yun Chen , Ying Ma , Jianqiang Hu , Aiqing Li
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引用次数: 0

摘要

聚集诱导的电化学发光(AIECL)发光团由于其在聚集状态下增强的电化学发光(ECL)发射而在生物传感应用中引起了极大的关注。然而,传统的AIECL系统由于其聚集的分子间力较弱,在生理环境中往往存在结构不稳定性。本文成功构建了包裹铂(II)-[4,2':6 ‘,4″-三吡啶]-4 ’ -羧酸(Pt(tpyc)2+)配合物的纳米限制性zr基金属有机骨架(PCN-777 mof),其中Pt(tpyc)2+)配合物通过Zr4+和- coo -配位键固定在PCN-777 mof中。得到的Pt- pcn -777 mof具有优异的结构稳定性和Pt(tpyc)2+的高负载效率。关键是,pcn -777- mof的介孔结构有效地促进了S2O82 -共反应物在其通道内的富集,显著缩短了固定Pt(tpyc)2+发射体和共反应物之间的电子转移途径。Pt-PCN-777 mof的协同效应加速了K2S2O8的分解,导致ECL信号增强。通过整合催化发夹组装和血红蛋白猝灭策略,构建了用于miRNA-21超灵敏检测的“Signal On-Off”ECL生物传感器。miRNA-21生物传感器具有优异的分析性能,如高灵敏度(约9.03 × 1017),宽线性范围(102-109 aM),飞摩尔级检测限(~ 66 aM),卓越的选择性和操作稳定性。本研究解决了传统AIECL系统不稳定性的局限性,有望建立一个多功能的Pt(II)-MOF复合材料平台,用于临床诊断先进的ECL探针。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Nanoconfined metal-organic frameworks encapsulating platinum(II) complexes with self-catalytic aggregation-induced electrochemiluminescence for ultrasensitive miRNA-21 biosensing
Aggregation-induced electrochemiluminescence (AIECL) luminophores have garnered significant attention for biosensing applications owing to their enhanced electrochemiluminescence (ECL) emission in aggregated states. However, conventional AIECL systems often suffer from structural instability in physiological environments due to weak intermolecular force of their aggregation. Here, we have successfully constructed nanoconfined Zr-based metal-organic frameworks (PCN-777 MOFs) encapsulating platinum(II)-[4,2':6′,4″-terpyridine]-4′-carboxylic acid (Pt(tpyc)2+) complexes, in which Pt(tpyc)2+) complexes are immobilized into PCN-777 MOFs via Zr4+ and -COO- coordination bond. The resulting Pt-PCN-777 MOFs achieved exceptional structural stability and high loading efficiency of Pt(tpyc)2+. Critically, the mesoporous structural of the PCN-777-MOFs efficiently proconcentrates of S2O82− co-reactant within its channels, significantly shortening electron-transfer pathway between immobilized Pt(tpyc)2+ emitters and coreactants. The synergistic effect of the Pt-PCN-777 MOFs accelerates K2S2O8 decomposition, leading to enhanced ECL signal. Through integrating catalytic hairpin assembly and hemin-quenching strategies, the "Signal On-Off" ECL biosensor is constructed for ultra-sensitive detection of miRNA-21. The miRNA-21 biosensor exhibits superior analytical performance, such as high sensitivity (approximately 9.03 × 1017), wide linear range (102–109 aM), femtomolar-level detection limit (∼66 aM), exceptional selectivity and operational stability. This study addresses the limitations of instability of traditional AIECL systems and is hopeful to establish a versatile platform of Pt(II)-MOF composites for advanced ECL probes in clinical diagnosis.
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来源期刊
Biosensors and Bioelectronics
Biosensors and Bioelectronics 工程技术-电化学
CiteScore
20.80
自引率
7.10%
发文量
1006
审稿时长
29 days
期刊介绍: Biosensors & Bioelectronics, along with its open access companion journal Biosensors & Bioelectronics: X, is the leading international publication in the field of biosensors and bioelectronics. It covers research, design, development, and application of biosensors, which are analytical devices incorporating biological materials with physicochemical transducers. These devices, including sensors, DNA chips, electronic noses, and lab-on-a-chip, produce digital signals proportional to specific analytes. Examples include immunosensors and enzyme-based biosensors, applied in various fields such as medicine, environmental monitoring, and food industry. The journal also focuses on molecular and supramolecular structures for enhancing device performance.
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