利用柠檬酸衍生碳点-硫化钨球的微流控纸基荧光传感装置用于现场检测塞硝唑。

IF 10.5 1区生物学 Q1 BIOPHYSICS

Biosensors and Bioelectronics Pub Date : 2025-09-23 DOI:10.1016/j.bios.2025.118025

Shan Arif , Mahjabeen Hassan , Sufian Rasheed , Naseer Ahmad , Batool Fatima , Syed Ghulam Musharraf , Dilshad Hussain

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引用次数: 0

摘要

塞克硝唑是第二代硝基咪唑，广泛用于治疗各种感染，包括细菌性阴道病、阿米巴病和贾第虫病，特别是治疗女性生殖器感染。本研究开发了一种基于智能手机的传感平台，结合基于微流控的纸张装置，通过荧光“关闭”，实现了塞克硝唑的可视化、快速、敏感和实时定量分析。除了对塞克硝唑的无标签和现场检测具有高灵敏度外，该传感装置还具有突出的抗干扰能力，可确保准确的现场识别。N-CDs@WS2-based荧光传感器对塞克硝唑的检测限为0.117 μM (117 nM)，定量限为0.3573 μM （357 nM）。该便携式传感器有效地实现了血清样品中塞克硝唑的实时监测，取得了令人鼓舞的结果，为其准确的现场检测铺平了道路。采用世界卫生组织（WHO）推荐的LC-MS和UPLC方法验证了传感器的检测效率。UPLC、LC-MS和N-CDs@WS2-based传感装置的回收率表明，该传感器可对实际样品中的塞硝唑进行定量分析。

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Microfluidic paper-based fluorescent sensing device using citric acid derived carbon dots-tungsten sulfide spheres for on-site detection of secnidazole

Secnidazole, a second-generation nitroimidazole, is widely used to treat various infections, including bacterial vaginosis, amoebiasis, and giardiasis, particularly in the treatment of female genital infections. Herein, a smartphone-based sensing platform has been developed in conjunction with a microfluidic-based paper device, enabling the visual, quick, sensitive, and quantitative analysis of secnidazole in real-time through fluorescence "Turn off". In addition to its high sensitivity for label-free and on-site detection of secnidazole, the proposed sensing device demonstrated a prominent anti-interference capability, ensuring accurate on-site identification. The N-CDs@WS₂-based fluorescent sensor exhibited a limit of detection (LOD) of 0.117 μM (117 nM) and a limit of quantification (LOQ) of 0.3573 μM (357 nM) for secnidazole. The portable sensor effectively enabled real-time monitoring of secnidazole in serum samples, yielding encouraging outcomes and paving the way for its accurate on-site detection. The detection efficiency of the sensor is validated by the World Health Organization (WHO) recommended LC-MS and UPLC methods. As indicated by the recoveries from UPLC, LC-MS, and N-CDs@WS₂-based sensing device, the proposed sensor can quantitaively analyze secnidazole in real samples.

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来源期刊

Biosensors and Bioelectronics 工程技术-电化学

CiteScore

20.80

自引率

7.10%

发文量

1006

审稿时长

29 days

期刊介绍： Biosensors & Bioelectronics, along with its open access companion journal Biosensors & Bioelectronics: X, is the leading international publication in the field of biosensors and bioelectronics. It covers research, design, development, and application of biosensors, which are analytical devices incorporating biological materials with physicochemical transducers. These devices, including sensors, DNA chips, electronic noses, and lab-on-a-chip, produce digital signals proportional to specific analytes. Examples include immunosensors and enzyme-based biosensors, applied in various fields such as medicine, environmental monitoring, and food industry. The journal also focuses on molecular and supramolecular structures for enhancing device performance.