Front-illuminated surface plasmon resonance biosensor for the study of light-responsive proteins and their interactions

Abstract

Light-responsive proteins are involved in a wide range of essential physiological processes in bacteria, plants, and animals. Engineered light-responsive proteins have also emerged as prospective tools in biotechnology and biomedicine. These proteins are often characterized by short-lived lit states and the need for continuous illumination to reach photostationary states. Therefore, developing methods for studying light-responsive proteins and their interactions under illumination represents an important research goal. Here, we report on a novel front-illuminated surface plasmon resonance (fiSPR) biosensor for monitoring interactions involving light-responsive proteins. The fiSPR biosensor combines the optical platform based on the Kretschmann geometry with advanced transparent microfluidics and an additional light module, enabling in situ illumination of the liquid sample in contact with the SPR chip. We apply the fiSPR biosensor to study the blue light-responsive transcription factor EL222, which recovers to the dark state in a few seconds and plays an important role in the optogenetic control of gene expression. Specifically, we determine the rate and equilibrium constants for EL222 dimerization and DNA binding. The results support the hypothesis that EL222 dimerizes prior to binding DNA. In addition, we provide evidence of the interaction between an interleukin receptor modified with a photocaged tyrosine (IL-20R2-Y70NBY) and its cytokine ligand (IL-24) only upon UV illumination. Overall, this study demonstrates the versatility of the developed fiSPR biosensor for monitoring biomolecular interactions involving both natural and engineered light-responsive proteins, particularly those featuring short lit-state lifetimes.