A Calix[4]arene-based wettability interface sensor for rapid ATP detection

IF 10.5 1区生物学 Q1 BIOPHYSICS

Biosensors and Bioelectronics Pub Date : 2025-09-16 DOI:10.1016/j.bios.2025.118001

Haonan Qu , Haifan Zhang , Cuiguang Ma , Qiang He , Ehsan Bahojb Noruzi , Xiangcheng Li , Guang Li , Haibing Li

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引用次数: 0

Abstract

ATP serves as a core indicator of cellular energy metabolism. The detection technology of ATP holds significant application value in fields such as biomedicine, microbiology, and bioengineering, and plays a critical role in advancing the development of precision medicine and biotechnology. A new bi-amide calix [4]arene (BAC4) was synthesized in a good yield of 85 % and was modified on a silicon surface via click chemistry to afford self-assembled monolayers (BAC4-SAMs), which was characterized by X-ray photoelectron spectroscopy (XPS) and water contact angle (CA). The BAC4-SAMs exhibits high selectivity to ATP over ADP and AMP, and its wettability can be modulated reversibly in the presence/absence of ATP between superhydrophobic and superhydrophilic states. At the concentration of 5.5 × 10⁻⁶ M, ATP remained detectable. The recognition mechanism is studied by UV–vis, ¹H NMR and AFM. Furthermore, the monitoring of ATP hydrolysis is successfully achieved via the BAC4-SAMs.

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一种用于ATP快速检测的杯状[4]芳烃润湿性界面传感器。

ATP是细胞能量代谢的核心指标。ATP检测技术在生物医学、微生物学、生物工程等领域具有重要的应用价值，对推进精准医学和生物技术的发展具有关键作用。以85%的收率合成了一种新的双酰胺杯芳烃（BAC4），并在硅表面通过点击化学修饰得到自组装单层（BAC4- sams），并用x射线光电子能谱（XPS）和水接触角（CA）对其进行了表征。BAC4-SAMs对ATP的选择性高于ADP和AMP，其润湿性可以在ATP存在或不存在的情况下在超疏水和超亲水状态之间进行可逆调节。在5.5 × 10-6 M的浓度下，ATP仍可检测到。通过紫外可见光谱、核磁共振氢谱和原子力显微镜研究了其识别机理。此外，通过bac4 - sam成功地实现了ATP水解的监测。

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来源期刊

Biosensors and Bioelectronics 工程技术-电化学

CiteScore

20.80

自引率

7.10%

发文量

1006

审稿时长

29 days

期刊介绍： Biosensors & Bioelectronics, along with its open access companion journal Biosensors & Bioelectronics: X, is the leading international publication in the field of biosensors and bioelectronics. It covers research, design, development, and application of biosensors, which are analytical devices incorporating biological materials with physicochemical transducers. These devices, including sensors, DNA chips, electronic noses, and lab-on-a-chip, produce digital signals proportional to specific analytes. Examples include immunosensors and enzyme-based biosensors, applied in various fields such as medicine, environmental monitoring, and food industry. The journal also focuses on molecular and supramolecular structures for enhancing device performance.