Bimodal optical fiber biosensor with high anti-interference based on two-photon polymerization 3D nanofabrication

IF 10.5 1区生物学 Q1 BIOPHYSICS

Biosensors and Bioelectronics Pub Date : 2025-09-06 DOI:10.1016/j.bios.2025.117952

Pengqi Gong , Xuegang Li , Xue Zhou , Yanan Zhang , Yong Zhao

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引用次数: 0

Abstract

This study develops a micro/nano dual-mode optical fiber sensor based on two-photon polymerization 3D processing technology. This sensor can simultaneously discriminate changes in bulk refractive index (BRI) caused by interferents and changes in surface refractive index (SRI) triggered by target analytes, making it suitable for biological detection in complex environments. The SRI detection of target biomolecules is achieved through surface plasmon resonance (SPR) assisted by surface biofunctionalization, while the BRI detection is accomplished through the optical path refractive index modulation mechanism of Fabry-Perot interference (FPI) in the resonant cavity. 3D microfabrication technology enables angular modulation of the conical waveguide and fabrication of the open resonant cavity, ensuring the simultaneous excitation of SPR and FPI effects within the micron-scale space. A pressure-driven microfluidic chip facilitates liquid-phase analysis with a sample volume of only 5 μL. Through refractive index solution tests and DNA hybridization experiments, the dual-mode BRI/SRI sensing characteristics were systematically characterized, with detection limits (DL) reaching 4 × 10⁻⁴ RIU and 1.2 nM. A DNA detection experiment using fetal bovine serum (FBS) was performed to simulate simultaneous BRI/SRI changes in biological detection scenarios. A matrix signal analysis method was proposed, which, when combined with dual-mode signal responses, enables accurate analysis of complementary DNA concentration with an error controlled within 3.7 %. In addition, the sensor's consistency and detection specificity across different environments were evaluated.

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基于双光子聚合三维纳米制造的高抗干扰双峰光纤生物传感器

本研究开发了一种基于双光子聚合三维加工技术的微纳双模光纤传感器。该传感器可以同时识别干扰引起的体折射率（BRI）变化和目标分析物引发的表面折射率（SRI）变化，适用于复杂环境下的生物检测。目标生物分子的SRI检测是通过表面等离子体共振（SPR）辅助表面生物功能化实现的，BRI检测是通过谐振腔中法布里-珀罗干涉（FPI）的光路折射率调制机制实现的。三维微加工技术可以实现锥形波导的角度调制和开放谐振腔的制造，确保在微米尺度空间内同时激发SPR和FPI效应。压力驱动的微流控芯片便于液相分析，样品体积仅为5 μL。通过折射率溶液测试和DNA杂交实验，系统表征了双模BRI/SRI传感特性，检测限（DL）达到4 × 10−4 RIU和1.2 nM。采用胎牛血清（FBS）进行DNA检测实验，模拟生物检测场景下BRI/SRI的同步变化。提出了一种矩阵信号分析方法，结合双模信号响应，可以准确分析互补DNA浓度，误差控制在3.7%以内。此外，还评估了传感器在不同环境下的一致性和检测特异性。

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来源期刊

Biosensors and Bioelectronics 工程技术-电化学

CiteScore

20.80

自引率

7.10%

发文量

1006

审稿时长

29 days

期刊介绍： Biosensors & Bioelectronics, along with its open access companion journal Biosensors & Bioelectronics: X, is the leading international publication in the field of biosensors and bioelectronics. It covers research, design, development, and application of biosensors, which are analytical devices incorporating biological materials with physicochemical transducers. These devices, including sensors, DNA chips, electronic noses, and lab-on-a-chip, produce digital signals proportional to specific analytes. Examples include immunosensors and enzyme-based biosensors, applied in various fields such as medicine, environmental monitoring, and food industry. The journal also focuses on molecular and supramolecular structures for enhancing device performance.