Trends in Analytical Chemistry最新文献

Minimalist nanobody architecture: A double-edged sword for immunological analysis 极简纳米体结构：免疫分析的双刃剑

IF 11.8 1区化学

Trends in Analytical Chemistry Pub Date : 2025-07-21 DOI: 10.1016/j.trac.2025.118382

Yulong Wang , Ruifeng Luo , Huijuan Yang , Pengyan Liu , Yaodong Zhang , Sa Dong , Xiude Hua , Bruce D. Hammock , Cunzheng Zhang

{"title":"Minimalist nanobody architecture: A double-edged sword for immunological analysis","authors":"Yulong Wang , Ruifeng Luo , Huijuan Yang , Pengyan Liu , Yaodong Zhang , Sa Dong , Xiude Hua , Bruce D. Hammock , Cunzheng Zhang","doi":"10.1016/j.trac.2025.118382","DOIUrl":"10.1016/j.trac.2025.118382","url":null,"abstract":"<div><div>A little over three decades developing, nanobodies (Nbs) are gaining increasing acceptance as analytical tools and promising therapeutic agents utilized within biological applications. They are well expressed in various microorganisms, easy to engineer, and highly robust to avoid storage deterioration and batch variance risks compared to that of conventional immunoglobulin G (IgG) antibodies, showing great promise as a new generation of commercial immunoreagents for analytical purpose. Nbs have remarkable characteristics that make them more favorable for use than conventional IgG antibodies or fragments thereof in certain areas of research. However, what is not widely appreciated is the two sides of their miniaturized structure when developing analytical methods. The progress made on advantages and limitations of miniaturized structure of Nb utilized in immunological analysis is reviewed in detail here. The goal is to offer the reader with (1) how Nb size enhances analytical performance; (2) in which scenarios limitations occur; and (3) how to mitigate these limitations. We focus in particular on the immobilization and bioconjugation chemistry to further amplify size contribution to analytical performance, as well as to compensate performance degradation results from miniaturized structure of Nbs.</div></div>","PeriodicalId":439,"journal":{"name":"Trends in Analytical Chemistry","volume":"192 ","pages":"Article 118382"},"PeriodicalIF":11.8,"publicationDate":"2025-07-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144702151","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Advances in mass spectrometry-based strategies for analyzing sialic acid linkage isomers 基于质谱分析唾液酸链异构体策略的研究进展

IF 11.8 1区化学

Trends in Analytical Chemistry Pub Date : 2025-07-20 DOI: 10.1016/j.trac.2025.118383

Linlin Wu, Mengmeng Wang, Chunfang Gao

{"title":"Advances in mass spectrometry-based strategies for analyzing sialic acid linkage isomers","authors":"Linlin Wu, Mengmeng Wang, Chunfang Gao","doi":"10.1016/j.trac.2025.118383","DOIUrl":"10.1016/j.trac.2025.118383","url":null,"abstract":"<div><div>Glycosylation is a crucial post-translational modification influencing protein function and disease development. Among glycan structures, α2,3- and α2,6-sialylated glycans exhibit distinct biological roles but are challenging to distinguish due to their structural similarity. Mass spectrometry (MS)-based techniques have become the indispensable platforms for sialic acid linkage isomers characterization, providing unparalleled sensitivity and rapid N-glycans profiling when synergized with complementary analytical strategies. This review focuses on the two main sialic acid linkage isomers, α2,3- and α2,6-, providing a brief overview of their roles in regulating biological functions and emphasizing the urgent need for their accurate detection. The review presents a detailed overview of recent advancements in MS-based methodologies for analyzing sialic acid linkage isomers, including non-derivatization, derivatization, and hybrid approaches. Furthermore, it highlights recent progress in glycomics and glycoproteomics data analysis, proposing theoretical insights to guide the selection of optimal methods for precise isomer identification and quantification.</div></div>","PeriodicalId":439,"journal":{"name":"Trends in Analytical Chemistry","volume":"191 ","pages":"Article 118383"},"PeriodicalIF":11.8,"publicationDate":"2025-07-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144696679","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Enzyme and nanozyme biosensors based on MOFs for pesticide detection 基于mof的酶和纳米酶生物传感器用于农药检测

IF 11.8 1区化学

Trends in Analytical Chemistry Pub Date : 2025-07-18 DOI: 10.1016/j.trac.2025.118381

Yijie Wang , Hongxia Li , Xu Yan

{"title":"Enzyme and nanozyme biosensors based on MOFs for pesticide detection","authors":"Yijie Wang , Hongxia Li , Xu Yan","doi":"10.1016/j.trac.2025.118381","DOIUrl":"10.1016/j.trac.2025.118381","url":null,"abstract":"<div><div>The prevailing trend in contemporary agricultural development is the adoption of smart agriculture, which heavily relies on precise pesticide management and monitoring to ensure crop health and environmental safety. In this context, the effectiveness of pesticide detection is directly related to the sustainability of agricultural production. Traditional strategies for pesticide detection still face challenges such as complicated operations and time-consuming processes, necessitating the development of more efficient detection methods. Recent advancements have seen the development of composites integrating metal-organic frameworks (MOFs) and enzymes, which leverage the strengths of both components to demonstrate remarkable catalytic activity and stability, thereby enhancing the efficacy of pesticide detection. In this review, the recent progress of MOF-based enzyme/nanozyme composites is systematically summarized in terms of the encapsulation modes of natural enzymes by MOFs and the catalytic type by MOFs as nanozymes. Furthermore, the applications of MOF-based enzyme/nanozyme composites in pesticide detection are reviewed. Based on this, the current challenges and future perspectives are discussed. It is desired that the review can provide further insights into the rational design of enzyme and nanozyme pesticide biosensors based on MOFs, thereby contributing to the advancement of smart agriculture.</div></div>","PeriodicalId":439,"journal":{"name":"Trends in Analytical Chemistry","volume":"191 ","pages":"Article 118381"},"PeriodicalIF":11.8,"publicationDate":"2025-07-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144680431","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Recent advances in biomass-based sorbents for the extraction of analytes in environmental, food, and biological samples 生物基吸附剂萃取环境、食品和生物样品中分析物的最新进展

IF 12 1区化学

Trends in Analytical Chemistry Pub Date : 2025-07-18 DOI: 10.1016/j.trac.2025.118377

Muhammad Sajid , Azizah A. Algreiby , Ghadah S. Alrebdi , Abdullah H. Alluhayb

{"title":"Recent advances in biomass-based sorbents for the extraction of analytes in environmental, food, and biological samples","authors":"Muhammad Sajid , Azizah A. Algreiby , Ghadah S. Alrebdi , Abdullah H. Alluhayb","doi":"10.1016/j.trac.2025.118377","DOIUrl":"10.1016/j.trac.2025.118377","url":null,"abstract":"<div><div>Biomass-based sorbents (BBSs) are gaining popularity because of their sustainability, cost-effectiveness, renewability, ease of synthesis, biodegradability, and unique structural characteristics, which also include naturally occurring functional groups. Depending on the nature of the analytes, they can be used in their raw or modified forms. Modifications may involve transforming them into carbonaceous materials or creating composites with other substances. BBSs have been extensively applied for the adsorptive removal of inorganic and organic pollutants from aqueous environments. Recently, there has been a growing trend in utilizing them for analytical sample preparation, driven by the increasing demand for sustainable materials in analytical procedures. They serve as extraction media in techniques like solid-phase microextraction, dispersive solid-phase extraction, magnetic solid-phase extraction, and more, primarily for concentrating analytes from environmental, food, and biological samples. This review examines this emerging trend, emphasizing the different forms of BBSs, their modifications, and their applications across various sample types.</div></div>","PeriodicalId":439,"journal":{"name":"Trends in Analytical Chemistry","volume":"192 ","pages":"Article 118377"},"PeriodicalIF":12.0,"publicationDate":"2025-07-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144721339","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Dual-mode SERS-based optical sensors for detection of contaminants in food: A comprehensive review 基于sers的双模光学传感器在食品中污染物检测中的应用综述

IF 11.8 1区化学

Trends in Analytical Chemistry Pub Date : 2025-07-17 DOI: 10.1016/j.trac.2025.118378

Shanshan Xue , Zhepeng Zhang , Lixin Ma , Pengmin Zhang , Chen Wang , Usman Majeed , Hesham R. El-Seedi , Xiaobo Zou , Zhiming Guo

{"title":"Dual-mode SERS-based optical sensors for detection of contaminants in food: A comprehensive review","authors":"Shanshan Xue , Zhepeng Zhang , Lixin Ma , Pengmin Zhang , Chen Wang , Usman Majeed , Hesham R. El-Seedi , Xiaobo Zou , Zhiming Guo","doi":"10.1016/j.trac.2025.118378","DOIUrl":"10.1016/j.trac.2025.118378","url":null,"abstract":"<div><div>Food safety is an escalating global issue with significant implications for public health. To combat this issue, dual-mode optical sensors based on Surface-Enhanced Raman Spectroscopy (SERS) have been proposed as the focus of scientific research. These sensors output two non-interfering optical signals that complement each other's strengths, offering enhanced detection capabilities. Hence, this review systematically explored recent advancements in SERS-based dual-mode optical sensors for detecting contaminants in food matrices, focusing on four prominent types. It outlined the principles of optical detection technology, described the sensing strategies of these sensors, and evaluated their performance in detecting food contaminants. The high sensitivity and reliability of these sensors are enabled by dual-signal verification, reducing misidentification in complex food matrices. Additionally, the review delved into existing challenges and proposed future directions. These advancements position SERS-based dual-mode sensors as powerful analytical tools for accurate, reliable contaminant detection, significantly improving food safety monitoring and quality control.</div></div>","PeriodicalId":439,"journal":{"name":"Trends in Analytical Chemistry","volume":"191 ","pages":"Article 118378"},"PeriodicalIF":11.8,"publicationDate":"2025-07-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144655155","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

From concept to application: Exploring trends and guiding developments in miniaturized sample preparation for food contaminant analysis 从概念到应用：探索趋势和指导食品污染物分析小型化样品制备的发展

IF 11.8 1区化学

Trends in Analytical Chemistry Pub Date : 2025-07-17 DOI: 10.1016/j.trac.2025.118376

Rafael Oliveira Martins, Maria Flávia Assunção Magalhães, Winnie Evelyn Valeria Perez Vite, Fernando Mauro Lanças

{"title":"From concept to application: Exploring trends and guiding developments in miniaturized sample preparation for food contaminant analysis","authors":"Rafael Oliveira Martins, Maria Flávia Assunção Magalhães, Winnie Evelyn Valeria Perez Vite, Fernando Mauro Lanças","doi":"10.1016/j.trac.2025.118376","DOIUrl":"10.1016/j.trac.2025.118376","url":null,"abstract":"<div><div>The excessive use of chemical compounds in agricultural practices has integrated these substances into daily life, raising significant concerns about their impact on human health. In response to these global concerns, numerous analytical strategies have been developed to monitor contaminants in food matrices effectively, aiming to detect and control chemical residues and ensure food safety. In light of this, solid and liquid-based miniaturized sample preparation strategies have been designed to address the complexities of food matrices, enabling accurate qualitative and quantitative analysis of trace-level compounds. These strategies effectively remove interfering endogenous compounds while simultaneously preconcentrating target analytes, ensuring the precise quantification of low contaminant concentrations in challenging food matrices. Despite their extensive applications in food matrix analysis, the literature lacks comprehensive and up-to-date reviews on these microextraction strategies for contaminant analysis in food. Moreover, a critical revision of the main advantages and challenges associated with extracting contaminants from food samples using miniaturized approaches is essential to provide readers with a comprehensive guide for selecting the most suitable method based on the sample type. Hence, this review study brings to light recent applications and innovations regarding versatile solid and liquid-based miniaturized extraction of different contaminants in food matrices.</div></div>","PeriodicalId":439,"journal":{"name":"Trends in Analytical Chemistry","volume":"191 ","pages":"Article 118376"},"PeriodicalIF":11.8,"publicationDate":"2025-07-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144680432","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Recent progress of biosensing techniques for environmental antibiotic resistance genes 环境抗生素耐药基因生物传感技术研究进展

IF 11.8 1区化学

Trends in Analytical Chemistry Pub Date : 2025-07-17 DOI: 10.1016/j.trac.2025.118380

Fangqi Wu , Qi Zhu , Liang Zhang , Wei Shen , Jingming Gong , Hian Kee Lee , Sheng Tang

{"title":"Recent progress of biosensing techniques for environmental antibiotic resistance genes","authors":"Fangqi Wu , Qi Zhu , Liang Zhang , Wei Shen , Jingming Gong , Hian Kee Lee , Sheng Tang","doi":"10.1016/j.trac.2025.118380","DOIUrl":"10.1016/j.trac.2025.118380","url":null,"abstract":"<div><div>The widespread use of antibiotics has led to the emergence of antibiotic resistant bacteria (ARB) and their resistance genes (ARGs) in the environment, posing a risk of transmission to humans. This trend complicates treatment and necessitates effective ARG detection methods for disease diagnosis and resistance prevention. Given the challenges of real-world detection environments, advancing biological detection technologies for precise identification of ARGs is crucial. Despite numerous reports on ARG detection, a comprehensive review of biosensors for ARG detection remains lacking. This review aims to introduce the latest research progress in the classification and application of biosensors for evaluating and monitoring ARGs, including electrochemical, photothermal and calorimetric sensors. The classification and characteristics of ARGs in the environment are summarized, and the mechanism and application of biosensors for detecting ARGs are discussed in detail. This review serves to supply comprehensive insights into biological detection methods for ARGs.</div></div>","PeriodicalId":439,"journal":{"name":"Trends in Analytical Chemistry","volume":"191 ","pages":"Article 118380"},"PeriodicalIF":11.8,"publicationDate":"2025-07-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144680433","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Boron nitride nanosheets for biosensors and biomedicine 用于生物传感器和生物医学的氮化硼纳米片

IF 11.8 1区化学

Trends in Analytical Chemistry Pub Date : 2025-07-17 DOI: 10.1016/j.trac.2025.118379

Guohai Yang , Luqing Chen , Jihan Liu , Yan Liu , Lulu Qu , Chengzhou Zhu , Jun-Jie Zhu , Yuehe Lin , Xiaochen Dong

{"title":"Boron nitride nanosheets for biosensors and biomedicine","authors":"Guohai Yang , Luqing Chen , Jihan Liu , Yan Liu , Lulu Qu , Chengzhou Zhu , Jun-Jie Zhu , Yuehe Lin , Xiaochen Dong","doi":"10.1016/j.trac.2025.118379","DOIUrl":"10.1016/j.trac.2025.118379","url":null,"abstract":"<div><div>The rapid development of advanced functional nanomaterials offers a robust foundation for exploring high-performance biosensing and biomedicine platforms. Boron nitride nanosheets (BNNS), atomically thin hexagonal boron nitride, have attracted significant attention due to outstanding chemical and thermal stability, excellent biocompatibility, and fascinating catalytic properties. Integrating doping and functionalization give BNNS-based materials significant potential in the fields of biosensors and biomedicine. This review firstly describes the structural features and overview of the prevailing preparation methods of BNNS. The following sections focus on the BNNS-based nanomaterials applications in biosensors. Additionally, the review addresses key biomedical applications of BNNS-based nanomaterials in drug delivery, boron neutron capture therapy (BNCT), and antibacterial treatments. Finally, the challenges faced by BNNS in these areas and potential pathways for future explorations are discussed. This review is expected to stimulate strategic advances in the use of BNNS for biosensors and biomedicine, thereby providing promising avenues for practical implementation.</div></div>","PeriodicalId":439,"journal":{"name":"Trends in Analytical Chemistry","volume":"191 ","pages":"Article 118379"},"PeriodicalIF":11.8,"publicationDate":"2025-07-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144680430","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Advances in solid-phase microextraction for detecting endocrine disrupting compounds in environmental and biological matrices 固相微萃取法检测环境和生物基质中内分泌干扰物的研究进展

IF 11.8 1区化学

Trends in Analytical Chemistry Pub Date : 2025-07-16 DOI: 10.1016/j.trac.2025.118372

Shaohan Wang , Jiaxing Yu , Yuemei Chen , Nan Li , Jiajia Han , Yang Yu , Yueru Shi , Guosheng Chen , Junlang Qiu , Fang Zhu , Gangfeng Ouyang

{"title":"Advances in solid-phase microextraction for detecting endocrine disrupting compounds in environmental and biological matrices","authors":"Shaohan Wang , Jiaxing Yu , Yuemei Chen , Nan Li , Jiajia Han , Yang Yu , Yueru Shi , Guosheng Chen , Junlang Qiu , Fang Zhu , Gangfeng Ouyang","doi":"10.1016/j.trac.2025.118372","DOIUrl":"10.1016/j.trac.2025.118372","url":null,"abstract":"<div><div>The widespread occurrences and adverse effects of endocrine disrupting compounds (EDCs) pose significant threats to the environment. Due to their persistence, bioaccumulation, and toxicity, EDCs migrate through environmental compartments and accumulate in organisms, resulting in health risks to humans. This concerning paradigm necessitates robust detecting strategies for EDCs across both environmental and biological matrices. Solid-phase microextraction (SPME) has emerged as a powerful analytical technique, particularly effective for complex matrix analysis. The miniaturization and non-exhaustive extraction characteristics of SPME make it uniquely suitable for both on-site and <em>in vivo</em> implementations. This review critically evaluates recent advances (2020–2025) in SPME applications for EDC detection in environmental and biological matrices. The discussion encompasses in-lab and on-site SPME approaches for environmental detecting, while also addressing <em>in vitro</em> and <em>in vivo</em> SPME methodologies in biological specimens. Finally, the future emerging trends and research directions in this field are proposed based on the recent achievements.</div></div>","PeriodicalId":439,"journal":{"name":"Trends in Analytical Chemistry","volume":"191 ","pages":"Article 118372"},"PeriodicalIF":11.8,"publicationDate":"2025-07-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144696649","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Towards sustainable analytical chemistry 走向可持续分析化学

IF 11.8 1区化学

Trends in Analytical Chemistry Pub Date : 2025-07-16 DOI: 10.1016/j.trac.2025.118371

Elefteria Psillakis

引用次数: 0