Journal of Chromatography A最新文献

Shared-autosampler parallel LC–MS/MS platform for high-throughput multi-analyte quantification in complex matrices 共享自动进样器并行LC-MS /MS平台，用于复杂矩阵中高通量多分析物定量

IF 4 2区化学

Journal of Chromatography A Pub Date : 2026-05-10 Epub Date: 2026-03-07 DOI: 10.1016/j.chroma.2026.466885

Shanshan Cai , Qisheng Zhong , Jiaqi Liu , Lisong Chen , Lingling Shen , Hongyuan Hao , Ting Zhou

{"title":"Shared-autosampler parallel LC–MS/MS platform for high-throughput multi-analyte quantification in complex matrices","authors":"Shanshan Cai , Qisheng Zhong , Jiaqi Liu , Lisong Chen , Lingling Shen , Hongyuan Hao , Ting Zhou","doi":"10.1016/j.chroma.2026.466885","DOIUrl":"10.1016/j.chroma.2026.466885","url":null,"abstract":"<div><div>Modern analytical tasks increasingly require the simultaneous quantification of large numbers of chemically diverse analytes in complex matrices, which remains challenging for a single LC–MS method. Here, we developed a shared-autosampler parallel LC–MS/MS strategy that enables two fully independent chromatographic methods to be executed sequentially within a single analytical cycle while generating one unified chromatogram-mass spectrum. The system employed two LC pump modules and two columns to form two independent LC pathways, which shared one autosampler and one MS detector for selective MRM acquisition and subsequent signal integration. Integration was performed exclusively at the MS detection and data-processing level, without inter-dimensional coupling or analyte transfer between columns. This avoided the mobile-phase compatibility and dead-volume limitations associated with 2D-LC, enabled modular recombination of chromatographic and MS conditions, and improved analytical throughput without additional MS detector or autosampler hardware. The performance of the strategy was demonstrated in two applications. An acidic/basic dual-method configuration enabled quantitative analysis of 394 emerging contaminants from 14 chemical classes in human serum within 28 min, exhibiting good linearity, limits of quantification not exceeding 0.20 ng/mL for >90 % of analytes, and peak-area RSDs generally below 10 %. A HILIC/RP parallel-column combination achieved simultaneous determination of 27 hypoglycemic agents spanning a log <em>P</em> range of −6.8 to 5.9 in a single 20 min run, reducing analysis time by >50 % compared with standard methods, with good precision and recoveries in spiked food samples. Overall, the shared-autosampler parallel LC–MS/MS strategy provided a flexible and efficient platform for high-throughput multi-analyte quantification in complex matrices.</div></div>","PeriodicalId":347,"journal":{"name":"Journal of Chromatography A","volume":"1774 ","pages":"Article 466885"},"PeriodicalIF":4.0,"publicationDate":"2026-05-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147387949","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Untargeted metabolomics and in-house database analysis reveal differences between elephantopus scaber L. and elephantopus tomentosus L. 非靶向代谢组学和内部数据库分析揭示了非洲象和毛象之间的差异。

IF 4 2区化学

Journal of Chromatography A Pub Date : 2026-05-10 Epub Date: 2026-03-06 DOI: 10.1016/j.chroma.2026.466881

Chang-Sheng Gao , Zhi-Kang Duan , Mei-Ya Lian, Run-Ze Yu, Ming Bai, Shu-Hui Dong, Shao-Jiang Song

{"title":"Untargeted metabolomics and in-house database analysis reveal differences between elephantopus scaber L. and elephantopus tomentosus L.","authors":"Chang-Sheng Gao , Zhi-Kang Duan , Mei-Ya Lian, Run-Ze Yu, Ming Bai, Shu-Hui Dong, Shao-Jiang Song","doi":"10.1016/j.chroma.2026.466881","DOIUrl":"10.1016/j.chroma.2026.466881","url":null,"abstract":"<div><div>In China, <em>Elephantopus scaber</em> L. and <em>Elephantopus tomentosus</em> L.<em>,</em> two traditional Chinese medicinal herbs commonly known as \"Ku-di-dan\", are widely used. Moreover, they have demonstrated anti-hepatoma effects and hepatoprotective properties in experimental studies. Nonetheless, according to existing literature, <em>E. scaber</em> and <em>E. tomentosus</em> encompass compounds with similar but notably different chemical structures. Therefore, investigating the differences in secondary metabolites between <em>E. scaber</em> and <em>E. tomentosus</em> and elucidating their distinct anti-hepatocellular carcinoma activities is of great importance. This study employed an integrated approach combining untargeted metabolomics with bioactivity assays to effectively differentiate between the congeneric plant species <em>E. scaber</em> and <em>E. tomentosus</em>. To improve the accuracy of characterizing and identifying key metabolites, an in-house database and data screening methods were employed. The research findings revealed that thirty major secondary metabolites were detected in <em>E. tomentosus</em> and <em>E. scaber</em> extracts through untargeted metabolomics and data screening. Subsequently, eight significant differential metabolites were identified through multivariate statistical analyses. Thereafter, twelve compounds were isolated from these two plants and evaluated for their anti-hepatocellular activity, revealing that compounds <strong>5</strong>—<strong>8</strong> exhibited significant inhibitory effects on HepG2 and Hep3B cells. Consistent results were also observed in the tests conducted on the crude extracts of both <em>E. scaber</em> and <em>E. tomentosus</em>. This study demonstrates that <em>E. scaber</em> and <em>E. tomentosus</em> exhibit distinct secondary metabolite profiles and differential anti-hepatocellular carcinoma activities.</div></div>","PeriodicalId":347,"journal":{"name":"Journal of Chromatography A","volume":"1774 ","pages":"Article 466881"},"PeriodicalIF":4.0,"publicationDate":"2026-05-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147387952","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Computer vision–based augmented visualisation for coffee origins identitation using comprehensive two-dimensional gas chromatography 基于计算机视觉的增强可视化的咖啡原产地鉴定使用全面的二维气相色谱。

IF 4 2区化学

Journal of Chromatography A Pub Date : 2026-05-10 Epub Date: 2026-02-21 DOI: 10.1016/j.chroma.2026.466836

Giorgio Felizzato , Eloisa Bagnulo , Giulia Tapparo , Giorgia Botta , Qingping Tao , Stephen E. Reichenbach , Chiara Cordero , Luciano Navarini , Erica Liberto , Andrea Caratti

{"title":"Computer vision–based augmented visualisation for coffee origins identitation using comprehensive two-dimensional gas chromatography","authors":"Giorgio Felizzato , Eloisa Bagnulo , Giulia Tapparo , Giorgia Botta , Qingping Tao , Stephen E. Reichenbach , Chiara Cordero , Luciano Navarini , Erica Liberto , Andrea Caratti","doi":"10.1016/j.chroma.2026.466836","DOIUrl":"10.1016/j.chroma.2026.466836","url":null,"abstract":"<div><div>Coffee is a highly complex and variable matrix, with volatile profiles shaped by multiple factors including botanical origin, climatic and soil conditions, post-harvest treatments, and roasting parameters. This variability generates complex chemical patterns, encompassing hundreds of volatile compounds from diverse chemical classes including pyrazines, furans, aldehydes, ketones, and terpenes. The resulting chemical dimensionality poses significant analytical challenges, making the accurate identification of characteristic compounds and reliable discrimination of coffee origins particularly difficult.</div><div>In this study, we applied comprehensive two-dimensional gas chromatography (GC×GC) coupled with computer vision (CV) to address these challenges. The workflow begins with untargeted fingerprinting, capturing all detectable compounds in a feature template. Multiple sample chromatograms are then combined into composite class images, representing the typical chemical features of each origin while minimising individual variability, which enables rapid pairwise comparison of different origins.</div><div>CV-based pairwise comparisons highlight differential peaks, which are integrated into a targeted template for subsequent peak extraction. Multivariate analyses then identify the key discriminant compounds driving origin differentiation. Post-processing strategies, such as ion-specific intensity mapping, further enhance interpretability, enabling visualisation of compositional differences across key chemical families. Overall, this GC×GC–CV workflow provides a robust, rapid, and visually intuitive platform for comprehensive chemical characterisation and origin classification of coffee, integrating untargeted and targeted analyses in a single framework.</div></div>","PeriodicalId":347,"journal":{"name":"Journal of Chromatography A","volume":"1774 ","pages":"Article 466836"},"PeriodicalIF":4.0,"publicationDate":"2026-05-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147353257","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Research advances in capillary gel electrophoresis for detecting biomacromolecules 毛细管凝胶电泳检测生物大分子的研究进展。

IF 4 2区化学

Journal of Chromatography A Pub Date : 2026-05-10 Epub Date: 2026-02-28 DOI: 10.1016/j.chroma.2026.466850

Wen-Jing Liu , Ying Zhang , Qiu-Yang Li , Rui-Ting Li , Long Zhao , Tharcisse Gatera , Qu-Huan Ma , Jun-Li Yang , Xiao-Feng Shi

引用次数: 0

A local predictive modelling approach for enhanced separation of critical volatile compounds with flow-modulated comprehensive two-dimensional gas chromatography 流动调制综合二维气相色谱法增强关键挥发性化合物分离的局部预测建模方法

IF 4 2区化学

Journal of Chromatography A Pub Date : 2026-05-10 Epub Date: 2026-03-07 DOI: 10.1016/j.chroma.2026.466886

Olga Vyviurska , Rocio Vidal Pellegrino , František Dugovič , Dominika Sklenárová , Ivan Špánik

{"title":"A local predictive modelling approach for enhanced separation of critical volatile compounds with flow-modulated comprehensive two-dimensional gas chromatography","authors":"Olga Vyviurska , Rocio Vidal Pellegrino , František Dugovič , Dominika Sklenárová , Ivan Špánik","doi":"10.1016/j.chroma.2026.466886","DOIUrl":"10.1016/j.chroma.2026.466886","url":null,"abstract":"<div><div>In recent years, the application of flow-modulated comprehensive two-dimensional gas chromatography (FM-GC × GC) has significantly increased, particularly for profiling complex food volatilomes. However, the full potential of this technique is often hindered by the complexity of instrumental optimisation, which is critical for achieving high-resolution separation across a wide variety of analytes in diverse samples. This work addresses this challenge by developing a predictive strategy to optimise FM-GC × GC conditions for the separation of a wide variety of volatile compounds. The efficiency of a local model based on peak parameters was tested across four different column setups, monitoring its correlation to the separation of 22 critical coeluting pairs. Using a Doehlert experimental design and a General Linear Model (GLM) approach, robust predictive models (R² > 0.80) were established and validated. Although the predictive models are confined to the tested experimental space, the underlying strategy and observed general behaviours provide a transferable framework for other setups. The use of a multiresponse optimisation strategy coupled to a global resolution metric, the Fraction of Resolved Peaks (FRP), successfully improved the separation of coelutions, particularly for specific column configurations. The effectiveness of this workflow was ultimately demonstrated by its successful application to the complex volatilomes of wine, honey, cascara coffee tea, and masala tea.</div></div>","PeriodicalId":347,"journal":{"name":"Journal of Chromatography A","volume":"1774 ","pages":"Article 466886"},"PeriodicalIF":4.0,"publicationDate":"2026-05-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147387955","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Archaea synthesize heterochiral phospholipid membranes as revealed by chiral analysis of archaeols 古菌的手性分析表明，古菌可合成杂手性磷脂膜。

IF 4 2区化学

Journal of Chromatography A Pub Date : 2026-05-10 Epub Date: 2026-03-01 DOI: 10.1016/j.chroma.2026.466853

Walter Hofmann , Andrea Palyzová , Jan Jansa , Nika Pende , Simon K.-M.R. Rittmann , Tomáš Řezanka

{"title":"Archaea synthesize heterochiral phospholipid membranes as revealed by chiral analysis of archaeols","authors":"Walter Hofmann , Andrea Palyzová , Jan Jansa , Nika Pende , Simon K.-M.R. Rittmann , Tomáš Řezanka","doi":"10.1016/j.chroma.2026.466853","DOIUrl":"10.1016/j.chroma.2026.466853","url":null,"abstract":"<div><div>This study comprehensively characterizes structural diversity of archaeol lipids in selected archaeal genera, integrating available genomic information with advanced lipidomic and stereochemical analyzes. Study of genomes from six archaeal genera revealed the presence of key enzymes involved in glycerophosphate biosynthesis, namely glycerol-1-phosphate dehydrogenase and glycerol-3-phosphate dehydrogenase. Their coexistence in some of the studied strains suggests the ability of these organisms to synthesize both enantiomeric forms of glycerol phosphates. Shotgun lipidomic profiling confirmed the presence of saturated and unsaturated archaeols, including extended forms (C20–C25), hydroxyarchaeols, and tetraether lipids, especially in the species <em>Haloferax volcanii, Methanopyrus kandleri,</em> and <em>Sulfolobus acidocaldarius</em>. Using on-line 2D LC-MS/MS, a specific molecular species of archaeol (20:3–25:4-AR) with seven double bonds was identified in <em>H. volcanii</em>, while bis-extended archaeol (C25–C25) was not detected. Chiral HPLC of a commercial archaeol standard resolved four isomers, with the (<em>R</em>)-2,3-di-<em>O</em>-phytanyl-<em>sn</em>-glycerol stereoisomer being the most abundant (> 80 %). In four of our archaeal strains a minor <em>S</em> enantiomer was present at concentrations of 6.8–13.9 % relative to the main <em>R</em> isomer, and its presence coincided with the presence of both glycerol phosphate dehydrogenases in the respective genomes. The distribution of these isomers across the studied strains supports the coexistence of both <em>R</em>- and <em>S</em>- enantiomers of archaeols in some, but not all, archaeal lineages. These findings highlight the stereochemical and structural complexity of archaeal membrane lipids and provide new insights into their biosynthetic diversity.</div></div>","PeriodicalId":347,"journal":{"name":"Journal of Chromatography A","volume":"1774 ","pages":"Article 466853"},"PeriodicalIF":4.0,"publicationDate":"2026-05-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147372138","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Chiral derivatization reagents for enantioselective LC-MS/MS analysis of amino acids with analyte-specific fragmentation 用于氨基酸对映选择性LC-MS/MS分析的手性衍生化试剂

IF 4 2区化学

Journal of Chromatography A Pub Date : 2026-05-10 Epub Date: 2026-02-28 DOI: 10.1016/j.chroma.2026.466851

Tatsuya Sakamoto, Kana Ishikura, Mayu Onozato, Takeshi Fukushima

{"title":"Chiral derivatization reagents for enantioselective LC-MS/MS analysis of amino acids with analyte-specific fragmentation","authors":"Tatsuya Sakamoto, Kana Ishikura, Mayu Onozato, Takeshi Fukushima","doi":"10.1016/j.chroma.2026.466851","DOIUrl":"10.1016/j.chroma.2026.466851","url":null,"abstract":"<div><div>Accurate enantioselective analysis of amino acids by liquid chromatography-tandem mass spectrometry (LC–MS/MS) remains challenging, as existing derivatization strategies often compromise either chromatographic resolution or structural discrimination in tandem mass spectrometry. In this study, we developed novel chiral derivatization reagents, (<em>R</em>)- and (<em>S</em>)-Oqic, based on a 1,3-oxazinoquinoline-4-one scaffold, for the simultaneous determination of amino acid enantiomers via LC–MS/MS. The reagents reacted rapidly with amino acids under mild basic conditions to form their chiral derivatives, enabling the simultaneous separation of 37 amino acids (18 pairs of amino acid enantiomers and Gly) on a conventional ODS column within 16 min. The resulting derivatives exhibited unique fragmentation patterns, yielding characteristic fragment ions at <em>m</em>/<em>z</em> 190 and [<em>M</em> + H − 171], with intensity ratios dependent on the tagged amino acid, thereby facilitating reliable analyte discrimination. The method was validated and subsequently applied to the analysis of commercially available fermented dairy products, revealing the presence of <span>d</span>-Ala, <span>d</span>-Ser, <span>d</span>-Asp, <span>d</span>-Glu, and <span>d</span>-Arg in yogurt and a probiotic beverage. The detection of sweet-tasting <span>d</span>-amino acids (<span>d</span>-Ala, <span>d</span>-Ser, and <span>d</span>-Arg) suggests their potential contribution to the sensory properties of fermented foods. Overall, the developed Oqic-based method provides a powerful tool for elucidating bacterial metabolism and food flavor profiles.</div></div>","PeriodicalId":347,"journal":{"name":"Journal of Chromatography A","volume":"1774 ","pages":"Article 466851"},"PeriodicalIF":4.0,"publicationDate":"2026-05-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147387951","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Metal-organic framework MIL-101(Cr) functionalized β-cyclodextrin-modified magnetic multi-walled carbon nanotubes for efficient extraction of amphenicol antibiotics 金属有机骨架MIL-101（Cr）功能化β-环糊精修饰磁性多壁碳纳米管高效提取抗生素

IF 4 2区化学

Journal of Chromatography A Pub Date : 2026-05-10 Epub Date: 2026-03-06 DOI: 10.1016/j.chroma.2026.466882

Lin Lv , Hongmei Liu , Tao Song , Yan Zhao

{"title":"Metal-organic framework MIL-101(Cr) functionalized β-cyclodextrin-modified magnetic multi-walled carbon nanotubes for efficient extraction of amphenicol antibiotics","authors":"Lin Lv , Hongmei Liu , Tao Song , Yan Zhao","doi":"10.1016/j.chroma.2026.466882","DOIUrl":"10.1016/j.chroma.2026.466882","url":null,"abstract":"<div><div>Given the extensive use of amphenicol antibiotics and their potential adverse effects on human health, the development of simple and highly sensitive detection methods is essential to ensure food safety. In this work, a novel and highly efficient magnetic sorbent based on β-cyclodextrin-modified magnetic multi-walled carbon nanotubes functionalized with MIL-101(Cr) (Fe<sub>3</sub>O<sub>4</sub>@β-CD@MWCNTs@MIL-101(Cr)) was developed and combined with ultra-high-performance liquid chromatography-mass spectrometry (UHPLC-MS/MS) to achieve highly sensitive detection of three amphenicol antibiotics (chloramphenicol, thiamphenicol, and florfenicol) in honey samples. The key factors influencing extraction efficiency in the magnetic solid-phase extraction process were identified using the Plackett-Burman experimental design, whereas the optimal experimental conditions were determined through Box-Behnken design-based response surface methodology optimization. Under these optimized conditions, favorable linear relationships for amphenicol antibiotics were observed within the concentration range of 0.5–250 ng mL<sup>−</sup><sup>1</sup> (R<sup>2</sup> ≥ 0.9951). The method exhibited detection limits of 0.04–0.07 μg kg<sup>−</sup><sup>1</sup>. Recovery rates ranged from 83.2% to 97.9%, with intra-day and inter-day precisions both below 7.2%. This method provides an efficient tool for monitoring amphenicol antibiotics in complex matrices.</div></div>","PeriodicalId":347,"journal":{"name":"Journal of Chromatography A","volume":"1774 ","pages":"Article 466882"},"PeriodicalIF":4.0,"publicationDate":"2026-05-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147387954","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Revisiting the definition and measurement of total accessible and hold-up volumes in liquid chromatography: Current understanding and advances 重新审视液相色谱中总可接近体积和持有体积的定义和测量：当前的理解和进展。

IF 4 2区化学

Journal of Chromatography A Pub Date : 2026-05-10 Epub Date: 2026-02-27 DOI: 10.1016/j.chroma.2026.466849

Judith Mollen , Gert Desmet , Deirdre Cabooter

{"title":"Revisiting the definition and measurement of total accessible and hold-up volumes in liquid chromatography: Current understanding and advances","authors":"Judith Mollen , Gert Desmet , Deirdre Cabooter","doi":"10.1016/j.chroma.2026.466849","DOIUrl":"10.1016/j.chroma.2026.466849","url":null,"abstract":"<div><div>The accurate determination of the retention factor, and particularly, the determination of the mobile phase volume that is needed for its computation, remains a fundamental, yet highly-debated topic in liquid chromatography. Two main approaches currently co-exist in literature: <em>i)</em> one based on the total accessible volume (= the total column volume accessible for solvent molecules after bonded phase coating) and <em>ii)</em> one based on the mobile phase volume that is accessible to unretained analytes (the hold-up volume). This review provides a clear graphical definition for both volumes and subsequently critically examines their use in current literature and reviews their methods of determination, emphasizing the need for careful experimental practice and standardized, universally accepted definitions. By comparing traditional experimental methods with findings from molecular dynamics simulations and Monte Carlo method studies, this review furthermore aims to clarify ambiguities and recommend best practices for future research. In addition, a comprehensive table linking the different names, symbols and definitions that have been used throughout literature to denote the different mobile phase volumes is provided, to help researchers navigate literature more easily.</div></div>","PeriodicalId":347,"journal":{"name":"Journal of Chromatography A","volume":"1774 ","pages":"Article 466849"},"PeriodicalIF":4.0,"publicationDate":"2026-05-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147353230","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Integrating natural deep eutectic solvents and ionic liquids as green mobile phase additives for enhanced selenium speciation analysis in food samples by liquid chromatography-atomic fluorescence spectrometry 结合天然深共晶溶剂和离子液体作为绿色流动相添加剂，液相色谱-原子荧光光谱法增强食品样品中硒的形态分析。

IF 4 2区化学

Journal of Chromatography A Pub Date : 2026-05-10 Epub Date: 2026-02-28 DOI: 10.1016/j.chroma.2026.466844

María B. Botella , María N. Oviedo , Rodolfo G. Wuilloud

{"title":"Integrating natural deep eutectic solvents and ionic liquids as green mobile phase additives for enhanced selenium speciation analysis in food samples by liquid chromatography-atomic fluorescence spectrometry","authors":"María B. Botella , María N. Oviedo , Rodolfo G. Wuilloud","doi":"10.1016/j.chroma.2026.466844","DOIUrl":"10.1016/j.chroma.2026.466844","url":null,"abstract":"<div><div>A study to evaluate the effects of natural deep eutectic solvent (NADES) and ionic liquids (ILs) on the separation of selenium (Se) species by reversed phase high performance liquid chromatography and detection by hydride generation atomic fluorescence spectrometry (RP-HPLC-HG-AFS) was performed in this work. The NADESs choline chloride:ethylene glycol and choline chloride:glycerol were applied as mobile phase additives for the separation of Se(IV), Se(VI), selenomethionine (SeMet) and Se-methylselenocysteine (SeMetSeCys). The effects of NADES composition, molar ratio, and concentration on chromatographic performance were systematically investigated, along with the influence of pH, buffer strength, and flow rate. The combined use of NADESs with the IL 1-hexyl-3-methylimidazolium chloride ([C<sub>6</sub>mim]Cl) significantly enhanced the resolution of inorganic Se species. In addition, the effects of NADES and IL on the Se fluorescence signals obtained with the HG-AFS techniques were also studied using a multivariate technique for sensitivity optimization. The method achieved limits of detection of 1.59–2.78 µg <span>L</span><sup>-1</sup>, with relative standard deviations of 0.53–1.86 % for retention times and 1.23–1.72 % for peak areas. Finally, the proposed analytical method was successfully applied to Se speciation analysis in complex food samples, including nuts and Se-enriched garlic, yielding recoveries of 93.4–106 %.</div></div>","PeriodicalId":347,"journal":{"name":"Journal of Chromatography A","volume":"1774 ","pages":"Article 466844"},"PeriodicalIF":4.0,"publicationDate":"2026-05-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147363700","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0