Journal of Chromatography A最新文献

{"title":"Epitope molecularly imprinted polymers based on host-guest interaction: specific recognition of CD59","authors":"Yitong Xu ,&nbsp;Huifeng Zhang ,&nbsp;Ying Zhang ,&nbsp;Jiutong Ma ,&nbsp;Qiong Jia","doi":"10.1016/j.chroma.2025.466056","DOIUrl":"10.1016/j.chroma.2025.466056","url":null,"abstract":"<div><div>The detection of CD59 is of great clinical importance since it is an important glycoprotein that can serve as a biomarker related to kinds of cancers. In this work, we prepared host-guest interaction based oriented epitope molecularly imprinted polymer (hg-EMIP) for the immobilization of CD59 N-terminal epitopes. Cucurbit[7]uril (CB[7]) and l-phenylalanine (L-phe) were employed as the host and guest; and tannic acid (TA) with abundant hydroxyl groups and diethylenetriamine (DETA) were chosen as the functional monomer and crosslinking agent, respectively. The obtained hg-EMIP can specifically recognize CD59 with the adsorption capacity of 88.2 mg/g and imprinting factor of 5.63, and possesses high reusability. The hg-EMIP-based method possesses a wide linear range (1 ng/mL - 1 μg/mL) and low limit of detection (0.44 ng/mL), and can be successfully used for the detection of CD59 in human serum sample. This study provides a scheme for the preparation of host-guest based epitope molecularly imprinted polymers for helping to identify potential disease biomarkers efficiently.</div></div>","PeriodicalId":347,"journal":{"name":"Journal of Chromatography A","volume":"1755 ","pages":"Article 466056"},"PeriodicalIF":3.8,"publicationDate":"2025-05-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144072011","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Magnetic thianthrene-based covalent organic frameworks with high stability and reusability for enrichment of mercury species at trace level","authors":"Jiahui Shi ,&nbsp;Zhenhua Wang ,&nbsp;Jingbo Chao ,&nbsp;Ligang Hu ,&nbsp;Bing Li ,&nbsp;Caifeng Dai","doi":"10.1016/j.chroma.2025.466049","DOIUrl":"10.1016/j.chroma.2025.466049","url":null,"abstract":"<div><div>Covalent organic frameworks (COFs) with low chemical or thermal stability may lead to destruction of their molecular structure when used under harsh conditions and environments, thereby limiting their use in practical processes. A magnetic COFs nano-adsorbent (Fe₃O₄@COF-TF) was prepared by coating thianthrene-based COFs with high chemical and thermal stability onto Fe₃O₄ nanoparticles by a simple solvothermal method without post-modification to introduce the target functional groups. Based on the above nano-adsorbent, a simple and practical magnetic solid-phase extraction–high-performance liquid chromatography–inductively coupled plasma mass spectrometry (MSPE-HPLC-ICP-MS) method was developed for the enrichment and determination of trace mercury species, including Hg²⁺, methylmercury (MeHg), and ethylmercury (EtHg). The experimental results showed that Fe₃O₄@COF-TF could still recover mercury species quantitatively after acid and alkali soaking treatment or 35 consecutive adsorption-desorption cycles. This indicates Fe₃O₄@COF-TF had excellent durability, extraction stability and reusability. Key parameters affecting the enrichment of mercury species, including solution pH, eluent composition and volume, adsorption and desorption time and dissolved organic matter, were systematically optimized. Under optimized conditions, the limits of detections (LODs) for Hg²⁺, MeHg and EtHg were 0.50, 0.19 and 0.67 ng <em>L</em>⁻¹ based on 300 mL of sample, with enrichment factors (EFs) of 338, 356 and 329, respectively. The accuracy and reproducibility of the method were validated through spiked tests and determination of certified reference materials. The developed method with the advantages of simplicity, high anti-interference capability, high enrichment factor and high sensitivity was successfully applied to the enrichment and determination of trace mercury in groundwater, surface water, seawater, and other complex matrices.</div></div>","PeriodicalId":347,"journal":{"name":"Journal of Chromatography A","volume":"1755 ","pages":"Article 466049"},"PeriodicalIF":3.8,"publicationDate":"2025-05-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144071855","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Ultrafast micromixer for on-line screening of antioxidants","authors":"Xinyi Zeng ,&nbsp;Xueying Zeng ,&nbsp;Xiaojun Feng ,&nbsp;Chunqin Wang ,&nbsp;Guangjun Fan ,&nbsp;Haiyan Zhong ,&nbsp;Youzhi Xu ,&nbsp;Bifeng Liu","doi":"10.1016/j.chroma.2025.466034","DOIUrl":"10.1016/j.chroma.2025.466034","url":null,"abstract":"<div><h3>Background</h3><div>The integration of high-performance liquid chromatography (HPLC) with rapid post-column biochemical detection techniques has gained widespread application in the pinpoint of antioxidant components in complex natural matrices. However, the use of PEEK reaction coils in on-line antioxidant analysis often encounters issues with poor mixing of the reaction solution, leading to peak broadening and a decrease in peak capacity. This may adversely affect the sensitivity and precision of the analysis. Microfluidic technology provides a unique opportunity for rapid solution mixing. Despite many continuous flow-based micromixers being reported, developing an ultrafast micromixer remains challenging.</div></div><div><h3>Results</h3><div>Here, we demonstrated a new microfluidic mixer and used it to create a highly sensitive on-line HPLC-ABTS system for antioxidant screening, replacing the PEEK reaction coil. The mixer utilizes the engulfment flow to achieve complete mixing of two solutions within sub-microseconds (∼0.6 µs). The system demonstrated consistent half-peak widths under 0.63 min for five phenolic compounds, with a 0.1 min delay time. Optimization of the HPLC-ABTS assay included determining the ideal ABTS^•+ concentration and flow rate ratio of ABTS^•+ solution to post-column mobile phase. Validation showed that the system exhibited low detection limits (0.3–1.4 μg/mL), superior linearity (<em>r</em> &gt; 0.9900), acceptable recovery (92.33–109.33 %), high stability (RSD &lt; 4.96 %), and excellent reproducibility (RSD &lt; 2.3 %). These observations highlight the advantages of employing the micromixer in on-line analysis.</div></div><div><h3>Significance</h3><div>Peak broadening reduces the selectivity and accuracy of on-line HPLC assays for detecting antioxidants in complex matrices. Compared to conventional on-line assays utilizing a PEEK coil for radical scavenging, our newly developed system demonstrates enhanced selectivity and precision. This on-line system can be combined with mass spectrometry or on-line extraction devices, which presents a promising alternative for the rapid screening of antioxidant compounds in plant products.</div></div>","PeriodicalId":347,"journal":{"name":"Journal of Chromatography A","volume":"1755 ","pages":"Article 466034"},"PeriodicalIF":3.8,"publicationDate":"2025-05-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144072010","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Flexible natural deep eutectic solvent extraction coupled with precolumn-switching HPLC-FLD to analyze fluorescent whitening agents in diverse sample matrices for migration study","authors":"Yuchuan Zheng ,&nbsp;Zikai Zheng ,&nbsp;Xianfeng Huang ,&nbsp;Mekhrdod S. Kurboniyon ,&nbsp;Rongfei Peng ,&nbsp;Jiaqi Liu ,&nbsp;Shijing Zhang ,&nbsp;Lingling Shen ,&nbsp;Xinguo Zhang ,&nbsp;Jialiang Pan","doi":"10.1016/j.chroma.2025.466047","DOIUrl":"10.1016/j.chroma.2025.466047","url":null,"abstract":"<div><div>This study proposes a novel analytical approach coupling of natural deep eutectic solvent (NADES) extraction with a precolumn-switching high-performance liquid chromatography with fluorescence detection (HPLC-FLD) system for analyzing fluorescent whitening agents (FWAs) in diverse sample matrices. NADES (coumarin/thymol) provide a green alternative to conventional solvents. The automated precolumn-switching strategy overcomes the compatibility issues between DES and HPLC, preventing column contamination and maintaining separation efficiency without the need for extensive dilution of NADES prior to injection. Three NADES extraction modes were proposed: phase-reversed NADES liquid-liquid extraction, NADES liquid-solid extraction, and NADES dispersive liquid-liquid microextraction, to achieve effective FWAs analysis in blood, urine, packaging materials, cosmetics, and cosmetic simulants. Method validation shows high sensitivity, with limits of detection (LODs) as low as 0.002 μg/L and good accuracy (recoveries 88.2 %–114.4 %). The practicality and reliability of this method are confirmed through its application to real positive samples from various source. Additionally, the research investigates FWAs migration from packaging to cosmetics and simulants, which also reveals the FWAs migration from sanitary products into blood and urine for the first time. The Weibull model has been proven to effectively describe FWAs migration behavior and provides a predictive tool for long-term migration risk assessment. This work not only provides a novel, green analytical approach for FWAs but also contributes valuable data for evaluating the safety of cosmetic packaging and sanitary products.</div></div>","PeriodicalId":347,"journal":{"name":"Journal of Chromatography A","volume":"1754 ","pages":"Article 466047"},"PeriodicalIF":3.8,"publicationDate":"2025-05-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143946602","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Rapid, high-throughput, and sensitive screening of herbal product metabolites in blood plasma using 2D carbon microfiber fractionation coupled with quadrupole time-of-flight high-resolution mass spectrometry: Huangkui capsule insights","authors":"Lei Yang ,&nbsp;Yu Qiu ,&nbsp;Miao Shao ,&nbsp;Meiyu Cui ,&nbsp;Meihua Dong ,&nbsp;Donatella Nardiello ,&nbsp;Maurizio Quinto ,&nbsp;Donghao Li","doi":"10.1016/j.chroma.2025.466043","DOIUrl":"10.1016/j.chroma.2025.466043","url":null,"abstract":"<div><div>Drug metabolite characterization from complex endogenous matrices such as plasma is a challenging task, especially for herbal product compounds. The analyses are generally carried out by flow-injection mass spectrometry (FI-MS) or combined with liquid chromatography (LC-MS), but the first one suffers from significant matrix effects, while the second is time-consuming and unsuitable for high-throughput analysis. In this study, a rapid method combining 2D-carbon microfiber fractionation (2DµCFs) with quadrupole time-of-flight high-resolution mass spectrometry (QTOF-HRMS) is proposed for the comprehensive characterization of herbal metabolites in plasma. Samples are separated into three fractions depending on their polarity (strong, medium, weak) and sequentially injected into MS, reducing matrix effects and enhancing metabolite coverage. Each 2DµCFs-QTOF-HRMS run takes only 5 min, enabling the detection of approximately 10,000 <em>m/z</em> features per injection—representing a 4-fold increase in the number of detected features compared to FI-MS. The proposed method maintains the high sensitivity offered by LC-MS, greatly simplifying the analytical procedure. By this approach, 14 potential effective compounds of Huangkui capsule (HKC), from dried flowers of <em>Abelmoschus manihot</em> (<em>A. manihot</em>), were characterized in blood plasma. Furthermore, the comparison of chemical components among different parts of <em>A. manihot</em> was performed, providing valuable insights into its active ingredients and creating a bridge between phytochemistry and clinical applications.</div></div>","PeriodicalId":347,"journal":{"name":"Journal of Chromatography A","volume":"1754 ","pages":"Article 466043"},"PeriodicalIF":3.8,"publicationDate":"2025-05-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143936886","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Streamlined purification of recombinant Alpha-1 antitrypsin from CHO cell culture using integrated chromatography within a single unit operation","authors":"Zi Ying Zheng ,&nbsp;Wen Qin Tang ,&nbsp;Say Kong Ng ,&nbsp;Wei Zhang","doi":"10.1016/j.chroma.2025.466046","DOIUrl":"10.1016/j.chroma.2025.466046","url":null,"abstract":"<div><div>Recombinant Alpha-1 Antitrypsin (A1AT) produced from CHO cell culture is a promising alternative to the commercially available drug, which is derived from the limited supply of blood plasma. By developing and optimizing the purification strategy through the combination of appropriate chromatography mechanisms and buffer conditions, we successfully achieved end-to-end purification of A1AT from cell culture supernatant to the final drug substance leveraging integrated chromatography within a single unit operation, with quality comparable or even superior to commercial drugs. With comparable processing time and buffer consumption, the proposed approach—integrating 2 anion exchange chromatography (AEX) and 1 hydrophobic charge induction chromatography (HCIC) —achieved 99.9 % purity and 27 ppm host cell protein (HCP), compared to 96.9 % purity and 2860 ppm HCP using the conventional method with a single AEX column in batch mode. Compared to the conventional approach of using one column per unit operation, this streamlined process can increase the productivity of A1AT by reducing unit operations and eliminating the need for sample holding and conditioning between chromatographic steps, thereby potentially reducing the manufacturing cost of recombinant A1AT produced from CHO cell culture.</div></div>","PeriodicalId":347,"journal":{"name":"Journal of Chromatography A","volume":"1754 ","pages":"Article 466046"},"PeriodicalIF":3.8,"publicationDate":"2025-05-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143936885","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Corrigendum to “Preparation of mixed-mode hydrophilic particle for efficient separation of common human milk oligosaccharides” [Journal of Chromatography A, Volume 1748, 10 May 2025, 465865]","authors":"Jingwei Kou ,&nbsp;Han Guo ,&nbsp;Jing Leng ,&nbsp;Houle Xiang ,&nbsp;Hui Wang ,&nbsp;Jinming Zhang ,&nbsp;Pengpeng Yang ,&nbsp;Fengxia Zou ,&nbsp;Wei Zhuang ,&nbsp;Huanqing Niu ,&nbsp;Hanjie Ying ,&nbsp;Jinglan Wu","doi":"10.1016/j.chroma.2025.466032","DOIUrl":"10.1016/j.chroma.2025.466032","url":null,"abstract":"","PeriodicalId":347,"journal":{"name":"Journal of Chromatography A","volume":"1753 ","pages":"Article 466032"},"PeriodicalIF":3.8,"publicationDate":"2025-05-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143943308","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Molecular insights into chromatography: Automated workflows for the virtual design of methacrylate-based chromatography resins","authors":"Tim Ballweg ,&nbsp;Modan Liu ,&nbsp;Ahmed Mama ,&nbsp;Wolfgang Wenzel ,&nbsp;Matthias Franzreb","doi":"10.1016/j.chroma.2025.466027","DOIUrl":"10.1016/j.chroma.2025.466027","url":null,"abstract":"<div><div>Computational chemistry provides invaluable insights into the behaviors and properties of various materials at the molecular level. This capability is of particular interest in chromatography where adsorbents engage with target molecules through intricate interactions. However, the broad integration of molecular simulations into the field of chromatography has been notably limited, despite significant achievements in previous studies. One potential reason is the requirement for considerable expertise to effectively configure these simulations, presenting a significant barrier to entry. In this context, workflow management systems (WMSs) provide a viable solution by allowing experts to automate complex simulation tasks, making them accessible to the wider research community without necessitating in-depth knowledge of the simulation process. This manuscript outlines the creation and application of two automated workflows designed to generate comprehensive all-atom models of methacrylate-based chromatography resin surfaces and to rapidly calculate binding free energies with peptides as target molecules. These innovations represent a significant advancement in the field by streamlining the simulation process, enhancing predictive accuracy, and making complex molecular modeling more accessible to researchers across disciplines. By publishing these workflows, we aim to catalyze molecular modeling in the field of chromatography by encouraging scientists to utilize and build upon our work.</div></div>","PeriodicalId":347,"journal":{"name":"Journal of Chromatography A","volume":"1754 ","pages":"Article 466027"},"PeriodicalIF":3.8,"publicationDate":"2025-05-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143943495","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Preparation and evaluation of versatile zwitterionic mixed-mode chromatographic stationary phases","authors":"Shuo Wang ,&nbsp;Yi-Ming Gu ,&nbsp;Jin Zhou ,&nbsp;Shu-Dong Wang","doi":"10.1016/j.chroma.2025.466024","DOIUrl":"10.1016/j.chroma.2025.466024","url":null,"abstract":"<div><div>The limitations of traditional single-mode stationary phases in complex chromatographic separations underscore the critical need for versatile mixed-mode stationary phases. Zwitterions, characterized by tunable ionic structures, have attracted considerable attention in the development of stationary phases. This study presents the innovative design of a bidentate phenyl-embedded zwitterionic stationary phase (SIL–BA–BMPPA), synthesized through the strategic integration of tertiary amine groups and carboxyl groups onto silica spheres. The retention mechanism of SIL–BA–BMPPA was comprehensively evaluated with analytes of diverse structures under various separation conditions. Its chromatographic performance was then compared with that of an alkyl-embedded counterpart (SIL–BA–BDA), as well as traditional C18 and bare silica stationary phases. As expected, SIL–BA–BMPPA exhibited satisfactory separation performance in both reversed-phase and hydrophilic interaction modes. Moreover, amphipathic analytes, including acidic, basic, and zwitterionic compounds, were efficiently separated on SIL–BA–BMPPA through the selective synergistic effects of multiple interactions. SIL–BA–BMPPA demonstrates versatile mixed-mode separation capabilities, endowing it with substantial potential for complex chromatographic separations.</div></div>","PeriodicalId":347,"journal":{"name":"Journal of Chromatography A","volume":"1754 ","pages":"Article 466024"},"PeriodicalIF":3.8,"publicationDate":"2025-05-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143936887","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Corrigendum to “Exploring accurate mass measurements in pixel-based chemometrics: Advancing coffee classification with GC-HRMS—A proof of concept study” [Journal of Chromatography A (2024) 465171]","authors":"Andre Cunha Paiva ,&nbsp;Carlos Alberto Teixeira ,&nbsp;Leandro Wang Hantao","doi":"10.1016/j.chroma.2025.466033","DOIUrl":"10.1016/j.chroma.2025.466033","url":null,"abstract":"","PeriodicalId":347,"journal":{"name":"Journal of Chromatography A","volume":"1753 ","pages":"Article 466033"},"PeriodicalIF":3.8,"publicationDate":"2025-05-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143943309","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}