Journal of Chromatography A最新文献

{"title":"Metal-organic framework MIL-101(Cr) functionalized β-cyclodextrin-modified magnetic multi-walled carbon nanotubes for efficient extraction of amphenicol antibiotics","authors":"Lin Lv ,&nbsp;Hongmei Liu ,&nbsp;Tao Song ,&nbsp;Yan Zhao","doi":"10.1016/j.chroma.2026.466882","DOIUrl":"10.1016/j.chroma.2026.466882","url":null,"abstract":"<div><div>Given the extensive use of amphenicol antibiotics and their potential adverse effects on human health, the development of simple and highly sensitive detection methods is essential to ensure food safety. In this work, a novel and highly efficient magnetic sorbent based on β-cyclodextrin-modified magnetic multi-walled carbon nanotubes functionalized with MIL-101(Cr) (Fe₃O₄@β-CD@MWCNTs@MIL-101(Cr)) was developed and combined with ultra-high-performance liquid chromatography-mass spectrometry (UHPLC-MS/MS) to achieve highly sensitive detection of three amphenicol antibiotics (chloramphenicol, thiamphenicol, and florfenicol) in honey samples. The key factors influencing extraction efficiency in the magnetic solid-phase extraction process were identified using the Plackett-Burman experimental design, whereas the optimal experimental conditions were determined through Box-Behnken design-based response surface methodology optimization. Under these optimized conditions, favorable linear relationships for amphenicol antibiotics were observed within the concentration range of 0.5–250 ng mL⁻¹ (R² ≥ 0.9951). The method exhibited detection limits of 0.04–0.07 μg kg⁻¹. Recovery rates ranged from 83.2% to 97.9%, with intra-day and inter-day precisions both below 7.2%. This method provides an efficient tool for monitoring amphenicol antibiotics in complex matrices.</div></div>","PeriodicalId":347,"journal":{"name":"Journal of Chromatography A","volume":"1774 ","pages":"Article 466882"},"PeriodicalIF":4.0,"publicationDate":"2026-05-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147387954","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Revisiting the definition and measurement of total accessible and hold-up volumes in liquid chromatography: Current understanding and advances","authors":"Judith Mollen ,&nbsp;Gert Desmet ,&nbsp;Deirdre Cabooter","doi":"10.1016/j.chroma.2026.466849","DOIUrl":"10.1016/j.chroma.2026.466849","url":null,"abstract":"<div><div>The accurate determination of the retention factor, and particularly, the determination of the mobile phase volume that is needed for its computation, remains a fundamental, yet highly-debated topic in liquid chromatography. Two main approaches currently co-exist in literature: <em>i)</em> one based on the total accessible volume (= the total column volume accessible for solvent molecules after bonded phase coating) and <em>ii)</em> one based on the mobile phase volume that is accessible to unretained analytes (the hold-up volume). This review provides a clear graphical definition for both volumes and subsequently critically examines their use in current literature and reviews their methods of determination, emphasizing the need for careful experimental practice and standardized, universally accepted definitions. By comparing traditional experimental methods with findings from molecular dynamics simulations and Monte Carlo method studies, this review furthermore aims to clarify ambiguities and recommend best practices for future research. In addition, a comprehensive table linking the different names, symbols and definitions that have been used throughout literature to denote the different mobile phase volumes is provided, to help researchers navigate literature more easily.</div></div>","PeriodicalId":347,"journal":{"name":"Journal of Chromatography A","volume":"1774 ","pages":"Article 466849"},"PeriodicalIF":4.0,"publicationDate":"2026-05-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147353230","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Integrating natural deep eutectic solvents and ionic liquids as green mobile phase additives for enhanced selenium speciation analysis in food samples by liquid chromatography-atomic fluorescence spectrometry","authors":"María B. Botella ,&nbsp;María N. Oviedo ,&nbsp;Rodolfo G. Wuilloud","doi":"10.1016/j.chroma.2026.466844","DOIUrl":"10.1016/j.chroma.2026.466844","url":null,"abstract":"<div><div>A study to evaluate the effects of natural deep eutectic solvent (NADES) and ionic liquids (ILs) on the separation of selenium (Se) species by reversed phase high performance liquid chromatography and detection by hydride generation atomic fluorescence spectrometry (RP-HPLC-HG-AFS) was performed in this work. The NADESs choline chloride:ethylene glycol and choline chloride:glycerol were applied as mobile phase additives for the separation of Se(IV), Se(VI), selenomethionine (SeMet) and Se-methylselenocysteine (SeMetSeCys). The effects of NADES composition, molar ratio, and concentration on chromatographic performance were systematically investigated, along with the influence of pH, buffer strength, and flow rate. The combined use of NADESs with the IL 1-hexyl-3-methylimidazolium chloride ([C₆mim]Cl) significantly enhanced the resolution of inorganic Se species. In addition, the effects of NADES and IL on the Se fluorescence signals obtained with the HG-AFS techniques were also studied using a multivariate technique for sensitivity optimization. The method achieved limits of detection of 1.59–2.78 µg <span>L</span>^-1, with relative standard deviations of 0.53–1.86 % for retention times and 1.23–1.72 % for peak areas. Finally, the proposed analytical method was successfully applied to Se speciation analysis in complex food samples, including nuts and Se-enriched garlic, yielding recoveries of 93.4–106 %.</div></div>","PeriodicalId":347,"journal":{"name":"Journal of Chromatography A","volume":"1774 ","pages":"Article 466844"},"PeriodicalIF":4.0,"publicationDate":"2026-05-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147363700","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Recent advances in greener alternative solvents for reversed-phase liquid chromatography in pharmaceutical analysis","authors":"Haile Kassahun ,&nbsp;Ann Van Schepdael ,&nbsp;Erwin Adams","doi":"10.1016/j.chroma.2026.466847","DOIUrl":"10.1016/j.chroma.2026.466847","url":null,"abstract":"<div><div>Reversed-phase liquid chromatography (RP-LC) is widely used in pharmaceutical analysis, often relying on traditional organic solvents which present significant environmental and health risks. As a result, the growing demand for sustainable analytical practices has led to the development of eco-friendly solvents for RP-LC. This review discusses recent developments in the application of greener alternative solvents, such as ethanol, glycerol, dimethyl carbonate, cyrene, acetone, propylene carbonate, ionic liquids, propylene glycol, deep eutectic solvents, and surfactants, in pharmaceutical analysis using RP-LC. The review also highlights the potential of these greener solvents to replace conventional ones in the analysis of pharmaceuticals. Overall, green solvents can achieve analytical performance comparable to traditional methods while significantly reducing environmental burden and health risks. The limitations and practical strategies to overcome the challenges are also discussed.</div></div>","PeriodicalId":347,"journal":{"name":"Journal of Chromatography A","volume":"1774 ","pages":"Article 466847"},"PeriodicalIF":4.0,"publicationDate":"2026-05-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147387930","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Development of a magnetic core–shell adsorbent-based microextraction method for the determination of pesticide residues in edible vegetables","authors":"Sina Mohammad Mehri ,&nbsp;Mohammad Reza Afshar Mogaddam ,&nbsp;Mir Ali Farajzadeh ,&nbsp;Mahboob Nemati","doi":"10.1016/j.chroma.2026.466839","DOIUrl":"10.1016/j.chroma.2026.466839","url":null,"abstract":"<div><div>For the first time, a new magnetic core-shell adsorbent (synthesized from Fe₃O₄, mercaptoacetic acid, and hexamethylene diamine) was introduced for the effective extraction of pesticide residues from agricultural products, including cucumber, tomato, and zucchini. The structural and magnetic characteristics of the synthesized adsorbent were fully confirmed through a series of instrumental techniques such as Fourier transform infrared spectrometry, scanning electron microscopy, Brunauer-Emmett-Teller, thermogravimetric analysis, X-ray diffraction, transmission electron microscopy, energy dispersive X-ray, vibrating sample magnetometer, and zeta-potential analysis. The combination of dispersive micro solid phase extraction with dispersive liquid–liquid microextraction enabled a highly efficient two-step sample preparation protocol with minimal solvent consumption and rapid operation. Due to the magnetic nature of the adsorbent, phase separation was rapidly achieved using an external magnet, eliminating the need for repeated centrifugation and significantly reducing extraction time. The optimized procedure utilized only 3 mg of the sorbent and μL volumes of environmentally benign organic solvents, making the method green and cost-effective. Under the optimized conditions, the method achieved excellent analytical performance, including low detection limits (0.31–2.65 µg L⁻¹), wide linear ranges, good repeatability (relative standard deviations of 1.1–6.3%), high enrichment factors (225–480), and acceptable extraction recoveries (45–96%). The method was successfully applied to real agricultural matrices, and relative recoveries within the 80–120% range confirmed the absence of significant matrix effects. Altogether, this study highlighted a powerful and innovative analytical platform that offered high sensitivity, selectivity, and operational simplicity for the determination of pesticide residues in complex food samples.</div></div>","PeriodicalId":347,"journal":{"name":"Journal of Chromatography A","volume":"1774 ","pages":"Article 466839"},"PeriodicalIF":4.0,"publicationDate":"2026-05-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147353255","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Feed injection–enabled reversed phase liquid chromatography for simplified analysis of lipophilic drugs and formulations","authors":"Daniel Foshag ,&nbsp;Stephan Buckenmaier ,&nbsp;Patrik Petersson","doi":"10.1016/j.chroma.2026.466887","DOIUrl":"10.1016/j.chroma.2026.466887","url":null,"abstract":"<div><div>Strong sample solvents and lipidic excipients hamper reversed‑phase (RP) liquid chromatography (LC) analysis of lipid‑based pharmaceutical formulations by causing solvent mismatch, resulting in peak distortion and restricting the applicable injection volume, which ultimately reduces the sensitivity to detect impurities. In this work, we evaluated feed injection (FI) under gradient conditions for the analysis of sesame oil-based formulations dissolved in ethyl acetate (EtOAc) and integrated FI with two‑dimensional LC coupled to mass spectrometry (2D‑LC/MS) for impurity profiling. Findings were compared to conventional flow‑through injection (FTI) and sandwich injection, across injection volumes of 1–40 µL. FI at low feed flow fractions (≤ 5 % of mobile phase flow rate) provided ≥ 1:20 online dilution, sustaining column head focusing, and preserving peak shape and linearity across the full injection range. In contrast, higher feed flow fractions (≥ 20 % of the mobile phase flow rate, ≤ 1:5 dilution) resulted in severe fronting peaks and non-linear ultraviolet (UV) detector response. Finally, the ²D transfers resolved matrix‑ and Active Pharmaceutical Ingredient (API)‑related impurities without laborious offline reconstitution or dilution. This work extends the application field of FI from analyte diluents to complex lipid matrices, offering a robust, scalable workflow for pharmaceutical formulation analysis.</div></div>","PeriodicalId":347,"journal":{"name":"Journal of Chromatography A","volume":"1774 ","pages":"Article 466887"},"PeriodicalIF":4.0,"publicationDate":"2026-05-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147387950","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Analysis of bio-nano interactions by electrical asymmetric flow field-flow fractionation with multiple online detectors","authors":"Panida Punnabhum ,&nbsp;Karim Daramy ,&nbsp;Napaporn Roamcharern ,&nbsp;Caterina Minelli ,&nbsp;Yiwen Pei ,&nbsp;Nelli Chourmouziadi Laleni ,&nbsp;Yvonne Perrie ,&nbsp;Zahra Rattray","doi":"10.1016/j.chroma.2026.466879","DOIUrl":"10.1016/j.chroma.2026.466879","url":null,"abstract":"<div><div>Understanding bio-nano interactions and protein corona formation is critical for advancing nanomedicines towards clinical translation. However, conventional methods for nanoparticle analysis have limited utility for <em>in situ</em> analysis due to interference from unbound proteins present in bulk biological media. Electrical asymmetric flow field-flow fractionation (EAF4), which integrates AF4 with an applied electrical field, enables size and surface charge-based separation, and when coupled with online detectors, provides simultaneous measurement of particle size, electrophoretic mobility, and zeta potential, key parameters governing bio-nano interactions. Here, we report the first application of multiplexed EAF4 with online detection for evaluating biophysical changes occurring in polystyrene latex and silk nanoparticles, used as model nanomedicine systems, following exposure to serum under conditions that mimic the protein composition of cell culture media. Our findings reveal significant alterations in particle physical attributes, including particle size, shape factor, zeta potential, and electrophoretic mobility following exposure to protein-containing media. Furthermore, we demonstrate that EAF4 enables gentle fractionation of complex biological samples, providing comprehensive physicochemical profiling of diverse particulate and macromolecular species within nanoparticle–protein complexes. This work establishes EAF4 as a powerful analytical platform for resolving nano–bio interactions and guiding the rational design of next-generation nanomedicines.</div></div>","PeriodicalId":347,"journal":{"name":"Journal of Chromatography A","volume":"1774 ","pages":"Article 466879"},"PeriodicalIF":4.0,"publicationDate":"2026-05-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147387953","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Microfluidics in separation science: Fabrication, applications and intelligent systems","authors":"Listya Eka Anggraini ,&nbsp;Muhammad Naeem Younis ,&nbsp;Mohammed A. Abdalmwla ,&nbsp;Ghadeer Ghannam Alharbi ,&nbsp;Khaoula El Yanboui ,&nbsp;Abdulaziz A. Al-Saadi ,&nbsp;Chanbasha Basheer","doi":"10.1016/j.chroma.2026.466852","DOIUrl":"10.1016/j.chroma.2026.466852","url":null,"abstract":"<div><div>The emergence of advanced, innovative microfluidic devices has reshaped analytical separation methodologies and biomolecular diagnostics by introducing highly integrated, miniaturized systems that enable next-generation fluid manipulation, enhanced automation, precision, cost efficiency, and high-throughput capabilities. Advances in fabrication methods, such as soft lithography, photolithography, and 3D printing, have enabled the production of customizable microfluidic platforms from materials such as PDMS, glass, and thermoplastics. These microfluidic systems support a range of analytical functionalities of the technologies, including gas chromatography (GC), high-performance liquid chromatography (HPLC), and electrophoresis, delivering rapid, high-resolution analyses with minimal sample and reagent requirements, quicker analysis, and enhanced uniformity and stability, facilitating high productivity screening, improved sensitivity and portability. Integration of micro-GC systems enables rapid, portable detection of volatile compounds, while microfluidic HPLC and electrophoresis provide precise biomolecular separations and real-time diagnostics. Recent advancements in artificial intelligence (AI) have now brought significant innovations in microfluidic devices, resulting in a new era of intelligent analytical separations, where machine learning algorithms and neural networks are applicable not only for data processing and pattern recognition but also for real-time optimization, anomaly detection, innovative read-out systems, and autonomous system control. The fusion of AI and microfluidics has enabled new approaches to dynamic experimentation, reshaping traditional microfluidics and personalized diagnostics and health care systems, where rapid, reliable analysis is paramount. In this review article, we comprehensively explain the progressive convergence of microfluidics and AI across various electrophoretic and chromatographic analytical separation techniques, elaborating on the latest technological innovations, fundamental fabrication strategies, and emergent applications. The article also presents a forward-looking perspective on how AI-enabled microfluidics enhances automation, scalability, and application-specific customization, which could shape the future of intelligent analytical separation systems.</div></div>","PeriodicalId":347,"journal":{"name":"Journal of Chromatography A","volume":"1774 ","pages":"Article 466852"},"PeriodicalIF":4.0,"publicationDate":"2026-05-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147353232","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Core-shell magnetic molecularly imprinted microspheres for selective extraction and determination of Schisandrol A with hepatoprotective evaluation in zebrafish larvae","authors":"Xiao Wang ,&nbsp;Hongle Wan ,&nbsp;Xinhua Li ,&nbsp;Ruya Wang ,&nbsp;Boyu Hao ,&nbsp;Wenchong Tang ,&nbsp;Mengyuan Zhang ,&nbsp;Wenbin Liu ,&nbsp;Mingyuan Gao ,&nbsp;Weidong Zhang","doi":"10.1016/j.chroma.2026.466784","DOIUrl":"10.1016/j.chroma.2026.466784","url":null,"abstract":"<div><div>A core–shell magnetic surface molecularly imprinted polymer (MIP) was synthesized using chitosan-modified Fe₃O₄ nanoparticles (Fe₃O₄@CS) as the support and Schisandrol A as the template for selective recognition. After optimization of adsorption conditions, static and dynamic binding experiments demonstrated high selectivity toward Schisandrol A, with an imprinting factor of 4.556. Based on this material, a magnetic solid-phase extraction coupled with high-performance liquid chromatography (MSPE–HPLC) method was established for the enrichment and quantitative determination of Schisandrol A in <em>Schisandra chinensis</em>, achieving a limit of detection of 0.59 ng/mL and a limit of quantification of 2.47 ng/mL. The practical applicability of the MIP was further evaluated by selectively depleting Schisandrol A from the herbal extract, followed by assessment of hepatoprotective activity using an ethanol-induced fatty liver zebrafish larva model. Comparative analysis of hepatic lipid accumulation and biochemical markers (AST, ALT, and SOD) revealed that removal of Schisandrol A significantly reduced the hepatoprotective efficacy of the extract, confirming its functional contribution. These results demonstrate that the proposed MIP-based MSPE–HPLC strategy provides a robust analytical tool for selective lignan analysis and enables component-specific functional evaluation in complex herbal systems.</div></div>","PeriodicalId":347,"journal":{"name":"Journal of Chromatography A","volume":"1773 ","pages":"Article 466784"},"PeriodicalIF":4.0,"publicationDate":"2026-04-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146172724","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Bottom-up synthesis of bridged Bis(β-cyclodextrin)-functionalized chiral COFs@SiO2 for enantioseparation in liquid chromatography","authors":"Wentao Xu ,&nbsp;Huizhen Ni ,&nbsp;Linna Xu ,&nbsp;Jiajun Gong ,&nbsp;Dongping Cheng ,&nbsp;Huawei Lv ,&nbsp;Xianchao Hu ,&nbsp;Chu Chu ,&nbsp;Xingnuo Li ,&nbsp;Shengqiang Tong","doi":"10.1016/j.chroma.2026.466782","DOIUrl":"10.1016/j.chroma.2026.466782","url":null,"abstract":"<div><div>Chiral covalent organic frameworks (CCOFs) have attracted considerable attention as promising materials for enantioseparation. In this work, a series of <em>β</em>-cyclodextrin-bridged chiral COFs with precisely tunable chiral-site densities were synthesized via a bottom-up strategy using the 1,3,5-tris(4-aminophenyl)benzene (TPB) linkers and newly designed BPTA-CD monomers. The BPTA-CD unit was synthesized by grafting mercapto-<em>β</em>-cyclodextrin (SH-<em>β</em>-CD) onto 2,5-bis(prop‑2-yn-1-yloxy) terephthalaldehyde (BPTA) via a thiol–alkyne “click” reaction. By adjusting the BPTA-CD/BPTA ratio, the density of chiral recognition sites within the COF framework could be finely regulated. The resulting CCOFs were covalently grafted onto silica microspheres to yield COF-grafted silica stationary phases for HPLC enantioseparation. Under optimized conditions, these stationary phases successfully separated 14 out of 19 dansylated amino acids (DNS-AAs) and exhibited high thermal stability and good reproducibility. Increasing the chiral-site density led to markedly enhanced enantioresolution, demonstrating the pivotal role of tunable chiral environments in recognition performance. This work provides an effective strategy for constructing chiral-site-controllable COFs for high-performance liquid chromatographic enantioseparation.</div></div>","PeriodicalId":347,"journal":{"name":"Journal of Chromatography A","volume":"1773 ","pages":"Article 466782"},"PeriodicalIF":4.0,"publicationDate":"2026-04-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146172726","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}