Journal of Chromatography A最新文献

{"title":"Monte Carlo simulation-based risk assessment of human exposure to multi-mycotoxins in Platycladi semen","authors":"Ling Fang ,&nbsp;Xiaokang Liu ,&nbsp;Lin Feng ,&nbsp;Kangnan Liu ,&nbsp;Zhenwei Li ,&nbsp;Yaling An ,&nbsp;Daidi Zhang ,&nbsp;Wenzhi Yang ,&nbsp;De-an Guo","doi":"10.1016/j.chroma.2025.465922","DOIUrl":"10.1016/j.chroma.2025.465922","url":null,"abstract":"<div><div>Mycotoxin contamination in <em>Platycladi semen</em> has become an increasing concern worldwide due to its significant threat to public health. In this study, a modified QuEChERS-based UPLC-MS/MS method was established to detect multi-mycotoxins in 190 batches of <em>Platycladi semen</em> samples obtained from various channels in China. Out of these samples, 188 tested positive for one or more mycotoxins, with an incidence of 98.95 %. Alternariol monomethyl ether was the most commonly detected with an incidence rate of 92.63 %, followed by fumonisin B₂ at 91.58 % and sterigmatocystin at 87.89 %. Notably, the aflatoxin B₁ contents in 26 samples and the total aflatoxins contents in 18 samples exceeded their respective official maximum residue limits. Given the toxicity and high contamination levels of mycotoxins, the risk of human exposure to them via the consumption of <em>Platycladi semen</em> was assessed using Monte Carlo simulation-based probabilistic models. The risk assessment using the margin of exposure and hazard quotient approach indicated that <em>Platycladi semen</em> is particularly susceptible to aflatoxins and sterigmatocystin, which pose significant threats to human health. This study provides the regulatory authority with a powerful platform for monitoring multi-mycotoxins in <em>Platycladi semen</em>, offering valuable data to safeguard consumers from potential health risks associated with mycotoxin contamination.</div></div>","PeriodicalId":347,"journal":{"name":"Journal of Chromatography A","volume":"1751 ","pages":"Article 465922"},"PeriodicalIF":3.8,"publicationDate":"2025-03-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143791440","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Potential of green solvents as mobile phases in liquid chromatography","authors":"Karen Gaudin","doi":"10.1016/j.chroma.2025.465810","DOIUrl":"10.1016/j.chroma.2025.465810","url":null,"abstract":"<div><div>This review summarizes the key points and focuses on the use of green solvents in reversed-phase high-performance liquid chromatography. Ethanol, acetone, ethyl lactate, propylene carbonate, dimethyl carbonate, methyl acetate, Cyrene, and glycerol are examined as green solvents, with an emphasis on their properties related to HPLC applications. A total of 135 articles published between 1990 and the present, which utilize ethanol-water mobile phases in RP-HPLC, highlight the established use of ethanol as a green solvent for RP-HPLC. Although ethanol is often characterized by its high viscosity and UV absorbance, it remains one of the most commonly used green solvents. This study shows that approximately 30 % of the ethanol-based methods developed employed columns with reduced particle diameters, without the need for column heating. In 26 % of cases, UV detection was used, even at wavelengths egal to or below 220 nm. However, ethanol's volatility and flammability pose risks of operator exposure and fire hazards. Consequently, alternative solvents have been explored to mitigate these issues.</div><div>Acetone, with over 20 years of use, presents similar safety concerns, compounded by its high UV absorbance. Recent advances in greener solvents, such as Cyrene, glycerol, and natural deep eutectic solvents, have been investigated to address VOC concerns in HPLC. However, these solvents still face challenges, including UV absorption, immiscibility with water, high viscosity, and limited availability in HPLC-grade quality. Therefore, additional research is needed to facilitate their broader application.</div></div>","PeriodicalId":347,"journal":{"name":"Journal of Chromatography A","volume":"1750 ","pages":"Article 465810"},"PeriodicalIF":3.8,"publicationDate":"2025-03-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143747595","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Green analytical approaches for contaminants: Sustainable alternatives to conventional chromatographic methods","authors":"Víctor Cutillas,&nbsp;Carmen Ferrer,&nbsp;María Jesús Martínez-Bueno,&nbsp;Amadeo R. Fernández-Alba","doi":"10.1016/j.chroma.2025.465921","DOIUrl":"10.1016/j.chroma.2025.465921","url":null,"abstract":"<div><div>The growing importance of environmental sustainability has driven advancements in analytical chemistry, particularly in the development of green chromatographic techniques. This review explores various chromatographic methods that align with green chemistry principles, offering environmentally friendly alternatives to traditional gold-standard approaches. Techniques such as supercritical fluid chromatography (SFC), miniaturized liquid chromatography (LC), and gas chromatography (GC) with alternative carrier gases (e.g., hydrogen and nitrogen) are discussed in depth for their ability to reduce solvent consumption, minimize waste, and improve operational efficiency. These methods not only maintain high sensitivity and accuracy but also reduce the ecological impact of contaminant analysis. By comparing these sustainable approaches to conventional techniques, this review highlights their potential to meet regulatory standards while supporting eco-friendly practices within routine analysis laboratories.</div></div>","PeriodicalId":347,"journal":{"name":"Journal of Chromatography A","volume":"1750 ","pages":"Article 465921"},"PeriodicalIF":3.8,"publicationDate":"2025-03-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143759297","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Improving the chromatographic separation of phosphorothioate oligonucleotide from impurities by optimizing selectivity through mobile-phase conditions in Ion-pair reversed-phase high-performance liquid chromatography","authors":"Yuki Obata,&nbsp;Hiroshi Sakamaki","doi":"10.1016/j.chroma.2025.465915","DOIUrl":"10.1016/j.chroma.2025.465915","url":null,"abstract":"<div><div>A diastereomer peak broadening has been observed in phosphorothioate (PS) oligonucleotide analysis in several modes of high-performance liquid chromatography (HPLC). PS oligonucleotide impurities are of various types, and their physicochemical properties are similar to those of PS oligonucleotides. Consequently, quality control requires the chromatographic separation of PS oligonucleotide from impurities and separation of impurities from one another, which is challenging. In this study, to optimize the selectivity for effectively separating PS oligonucleotide from its impurities such as phosphodiester impurities (POs) and <em>n</em> − 1 truncated sequences (<em>n</em> − 1 s) in ion-pair reversed-phase (IP-RP) HPLC, the effect of mobile-phase conditions on the selectivity was investigated. Most importantly, it was demonstrated that the selectivity for the diastereomers of PS oligonucleotides is optimally reduced by the ion-pair (IP) system using alkylamine with a tertiary or quaternary structure and alkyl chain lengths of ≥4, using a method of observing the peak widths of PS oligonucleotides. Alternatively, using counterion species, such as hydrophobic alkyl carboxylic acid, improves the selectivity between the PS oligonucleotide and its impurities. Consequently, the IP system using tributylamine and heptanoic acid is proposed as the system that has optimal selectivity. This system can separate PS oligonucleotides from spiked PO and <em>n</em> − 1 groups involving those with different positions and has outstanding quantitative performance at the 0.2 %–5 % range. This study provides a guidance for optimizing the selectivity for IP-RP HPLC, and the proposed IP system could be useful for ensuring the quality control of antisense oligonucleotides including PS linkages.</div></div>","PeriodicalId":347,"journal":{"name":"Journal of Chromatography A","volume":"1750 ","pages":"Article 465915"},"PeriodicalIF":3.8,"publicationDate":"2025-03-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143777135","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Sulfonic acid-functionalized covalent organic framework@Ti3C2Tx as efficient solid-phase microextraction blade coating for the extraction of monoamine neurotransmitters in rat serum samples","authors":"Cheng Yang ,&nbsp;Nian Yang ,&nbsp;Di Zhao ,&nbsp;Zhiyu Zhang ,&nbsp;Junying Song ,&nbsp;Zhenqiang Zhang ,&nbsp;Kai Hu ,&nbsp;Shusheng Zhang","doi":"10.1016/j.chroma.2025.465919","DOIUrl":"10.1016/j.chroma.2025.465919","url":null,"abstract":"<div><div>Herein, a sulfonic-functionalized covalent organic framework@Ti₃C₂T<em>_x</em> nanocomposite (SO₃H<img>COF@Ti₃C₂T<em>_x</em>) was synthesized and employed as solid phase microextraction (SPME) coating for isolation and extraction of monoamine neurotransmitters (MNTs) from rat serum samples. The resultant composite can combine the characteristics of hydrophilic Ti₃C₂T<em>_x</em> and SO₃H<img>COF, which endow it has multiple adsorption sites and can provide multiple interactions such as cation exchange, hydrogen bonding and π-π with the target MNTs. The synthesized SO₃H<img>COF@Ti₃C₂T<em>_x</em> SPME blades have excellent protein exclusion capability, ensuring high adsorption efficiency for MNTs. Under the optimized conditions, the proposed SO₃H<img>COF@Ti₃C₂T<em>_x</em> blades-based SPME-HPLC method exhibited good linearities (<em>r</em>²≥0.9963), low limits of detection (0.015–0.030 ng mL^-1) and low matrix effect (0.83 %-17.36 %). The recoveries of MNTs in the rat serum were in range of 90.3 %-118.3 %, with RSDs &lt;10.8 %. The SPME-HPLC method was successfully applied for the analysis of 4 MNTs in the serum of depression model rats. This work not only details the development of a multi-functional composite, but it also presents an effective strategy for the determination of trace MNTs in serum sample.</div></div>","PeriodicalId":347,"journal":{"name":"Journal of Chromatography A","volume":"1750 ","pages":"Article 465919"},"PeriodicalIF":3.8,"publicationDate":"2025-03-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143747598","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Fabrication and evaluation of a dual-zwitterionic functionalized hydrophilic monolith for chromatographic separation","authors":"Liang Lai ,&nbsp;Hongying Lan ,&nbsp;Hui Huang ,&nbsp;Tong Li ,&nbsp;Kesara Ar-sanork ,&nbsp;Jia-Huan Qu ,&nbsp;Dongsheng Xu ,&nbsp;Zhengjin Jiang","doi":"10.1016/j.chroma.2025.465917","DOIUrl":"10.1016/j.chroma.2025.465917","url":null,"abstract":"<div><div>In this study, the chromatographic performance of a novel dual-zwitterionic hydrophilic stationary phase were investigated. Three zwitterionic HILIC monoliths were prepared using 3-(4-((methacryloyloxy)methyl)-1-methylpiperidin-1-ium-1-yl)propane-1-sulfonate (MAMMPS), 2-methacryloyloxyethyl phosphorylcholine (MPC), and a combination of MAMMPS and MPC (1:1, molar ratio) with <em>N,N'</em>-methylenebisacrylamide (MBA), separately. The monoliths exhibited good repeatability and stability. A high column efficiency of 120,000 plates/m was reached on the novel dual-zwitterionic functionalized poly(MAMMPS@MPC-<em>co</em>-MBA) monolith. Through the analysis and testing with different standards, the poly(MAMMPS@MPC-<em>co</em>-MBA) monolith exhibited the strongest retention capacity for negatively charged benzoic acid derivatives due to its high hydrophilicity and weak electronegativity. In contrast, the poly(MPC-<em>co</em>-MBA) monolith, functionalized with a single zwitterion, facilitated electrostatic interactions with negatively charged analytes. Baseline separation was achieved on all three monoliths for selected nucleobases, nucleosides, phenol derivatives, and amine compounds. However, their retention strength was mainly related to the hydrophilicity of the stationary phases, while hydrogen bonding and electrostatic interactions played secondary roles. Among the monoliths, the poly(MAMMPS-<em>co</em>-MBA) monolith demonstrated the best separation for neutral, acidic, and alkaline compounds. These findings offer valuable insights for the future selection and application of dual-zwitterionic HILIC monoliths for chromatographic separations.</div></div>","PeriodicalId":347,"journal":{"name":"Journal of Chromatography A","volume":"1751 ","pages":"Article 465917"},"PeriodicalIF":3.8,"publicationDate":"2025-03-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143785293","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Development of a highly sensitive field-amplified capillary electrophoresis tandem triple quadrupole mass spectrometry for the quantification of trace methamphetamine in sewage","authors":"Yanping Wei ,&nbsp;Yujie Zhang ,&nbsp;Yueting Hu ,&nbsp;Fan Shui ,&nbsp;Jia Tang ,&nbsp;Yiyang Yu ,&nbsp;Guocan Zheng ,&nbsp;Jing Zeng","doi":"10.1016/j.chroma.2025.465920","DOIUrl":"10.1016/j.chroma.2025.465920","url":null,"abstract":"<div><div>Accurate quantification of trace levels of illicit drugs in wastewater, such as methamphetamine (MAMP), is crucial for sewage epidemiology. In this study, we developed a novel method that integrates field-amplified sample injection capillary electrophoresis with triple quadrupole mass spectrometry (FASI-CE-QQQ MS). This method enables simultaneous on-line preconcentration and separation, coupled with highly sensitive detection. Validation demonstrated excellent linearity within the range of 1 to 200 ng/L (R² = 0.9996), a limit of detection (LOD) of 0.1 ng/L (S/<em>N</em> = 12.3), high accuracy (RE &lt; 6.4 %) and precision (RSD &lt; 11.4 %). It was successfully applied to quantify MAMP in two unknown water samples, yielding average concentrations of 3.70 ng/L and 84.73 ng/L, respectively. These results were consistent with those obtained from an interlaboratory comparison exercise across China using on-line solid-phase extraction coupled with ultra-high performance liquid chromatography tandem triple quadrupole mass spectrometry (on-line SPE-UHPLC-QQQ MS). Finally, a comprehensive comparison between the newly developed FASI-CE-QQQ MS and the on-line SPE-UHPLC-QQQ MS methods revealed that the FASI-CE-QQQ MS offers significant advantages in terms of sensitivity, rapidity, and reduced solvent and sample consumption, making it a promising approach for the accurate determination of trace drugs in water.</div></div>","PeriodicalId":347,"journal":{"name":"Journal of Chromatography A","volume":"1750 ","pages":"Article 465920"},"PeriodicalIF":3.8,"publicationDate":"2025-03-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143747596","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Ion exchange- and enrichment-based technology applied to large-scale plasma proteomic analysis of breast cancer neoadjuvant chemotherapy","authors":"Xiaozhen Cui ,&nbsp;Zhihua Zhong ,&nbsp;Sen Xu ,&nbsp;Yini Pan ,&nbsp;Xi Wang ,&nbsp;Luobin Zhang ,&nbsp;An He ,&nbsp;Xueting Ye ,&nbsp;Hua Cao ,&nbsp;Weibing Zhang ,&nbsp;Ruijun Tian","doi":"10.1016/j.chroma.2025.465914","DOIUrl":"10.1016/j.chroma.2025.465914","url":null,"abstract":"<div><div>Mass spectrometry (MS) based proteomics provides unbiased quantification of all proteins in plasma, which can dynamically reflect individual health states in real time. However, large-scale proteomics studies are constrained by the excessive dynamic range of plasma proteome and low throughput. Herein, two kinds of magnetic metal-organic frameworks (MOFs) modified with ion exchange functional groups (denoted as MHP-UiO-66-SAX and MHP-HKUST-1-SCX) were designed and fabricated to exhibit large protein adsorption capability, which were combined with an automated Liquid-handling System, thus realizing in-depth, high-throughput and automated proteomics studies. The constructed workflow could automatically complete the sample preparation before MS within only six hours and nearly a thousand protein groups per sample could be quantified. In the cohort study of nearly one hundred breast cancer neoadjuvant chemotherapy (NC) plasma samples, two differentially expressed proteins previously reported as biomarkers were related with the pathological complete response (PCR) of the breast cancer, demonstrating the feasibility of the developed technology for preparing large-scale clinical samples and exhibiting the potential application in monitoring the effect of chemotherapy.</div></div>","PeriodicalId":347,"journal":{"name":"Journal of Chromatography A","volume":"1750 ","pages":"Article 465914"},"PeriodicalIF":3.8,"publicationDate":"2025-03-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143783028","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Model-based analysis of a hydrophobic interaction chromatography for antibody-drug conjugate purification","authors":"Tobias Hahn ,&nbsp;Fara Lyu ,&nbsp;Pia Graf ,&nbsp;Steve Richter ,&nbsp;Jorge Gandarilla ,&nbsp;Dean Clyne ,&nbsp;Lei Cao ,&nbsp;Chen Wang","doi":"10.1016/j.chroma.2025.465916","DOIUrl":"10.1016/j.chroma.2025.465916","url":null,"abstract":"<div><div>Antibody-drug conjugates (ADCs) are a class of biopharmaceutical drugs designed as a targeted therapy for treating cancer while sparing healthy cells. ADCs are complex molecules composed of an antibody linked to a biologically active cytotoxic drug. The drug-to-antibody ratio (DAR), which represents the number of drugs conjugated to an antibody, is an important quality attribute of ADCs. The conjugation process typically yields a complex DAR profile, which requires further purification to remove undesired DAR species. Separation of DAR species post conjugation reaction can be achieved using hydrophobic interaction chromatography (HIC). HIC utilizes the hydrophobicity differences of different drug-loaded ADCs to separate them by reversible interaction between the proteins and the hydrophobic stationary phase. The buffer greatly influences the binding interaction between hydrophobic proteins and a HIC resin, but the process is also sensitive to variations in temperature, resin attributes, and the solid-liquid ratio of the column. A mechanistic model that captures these critical process parameters and material and column properties was established from calibration studies using multiple well-characterized HIC columns and resin lots. This model enabled in-silico characterization of the HIC process and facilitated understanding of unexpected process performance observations. It revealed the importance of controlling resin-specific hydrophobic capacity to minimize the DAR separation variations. It led to identifying an effective process control strategy to ensure a consistent DAR profile.</div></div>","PeriodicalId":347,"journal":{"name":"Journal of Chromatography A","volume":"1750 ","pages":"Article 465916"},"PeriodicalIF":3.8,"publicationDate":"2025-03-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143747594","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Off-line in-tube solid-phase microextraction of some herbicides based on poly(styrene-divinylbenzene) coated Fe3O4 nanoparticles","authors":"Maedeh Saadat ,&nbsp;Yadollah Yamini ,&nbsp;Nasser Nikfarjam ,&nbsp;Hanieh Kefayati","doi":"10.1016/j.chroma.2025.465910","DOIUrl":"10.1016/j.chroma.2025.465910","url":null,"abstract":"<div><div>Triazines are a class of persistent micropollutants in environmental areas with high toxicity. Because of their general usage and poisonous and stability of their deteriorated products, extraction and determination trace amounts of triazines are important. Off-line in-tube solid phase microextraction efficiently used for extraction of trace amounts of analytes. In addition, magnetic field was used to keep the nanoparticles in the tube and also to enhance preconcentration of the analytes. Magnetic nanoparticles can be utilized as a reusable sorbent with high surface area, strong interaction, chemical stability and high selectivity.</div><div>A novel approach was introduced using a magnetic nanocomposite of cross-linked poly(styrene-divinylbenzene) for the in-tube solid-phase microextraction of triazine herbicides. The prepared material was characterized using various techniques. High performance liquid chromatography-ultraviolet detection was used to separate and determine the analytes. The effects of pH (6), extraction time (20 min), type and volume of elution solvent (500 µL methanol) and extraction flow rate (2 mL min^-1), desorption flow rate (50 µL min^-1) and salt addition (15%) and applying magnetic field on the extraction efficiency of the triazines were investigated. Under optimum conditions, calibration curves were found to be linear in the range of 0.3-500 µg L⁻¹ for cyanazine, simazine, and propazine with a coefficient of determination (R²) of 0.9951, 0.9984, and 0.9984, respectively. The LODs were 0.1 µg L⁻¹ for all of the analytes. The method was successfully used for the extraction and determination of triazine herbicides in fruit juice samples.</div><div>Magnetic nano particles illustrate minimum back pressure in tube due to elimination of filters, high surface-to-volume ratio and lot of active sites favorable for the adsorption of the analytes. Despite the limitations imposed by the chemical conditions of extraction on the application of nanoparticles and polymeric sorbents, variables such as pH, concentration, and surface charges do not influence the magnetic interactions of MNPs. By applying magnetic field, the magnetic nature of the analytes is effective on the preconcentration of them.</div></div>","PeriodicalId":347,"journal":{"name":"Journal of Chromatography A","volume":"1750 ","pages":"Article 465910"},"PeriodicalIF":3.8,"publicationDate":"2025-03-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143747597","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}