Journal of Chromatography A最新文献

{"title":"An ultra-high-performance tandem mass spectrometry method to quantify tryptophan metabolites in aqueous humor of primary angle-closure glaucoma patients","authors":"Xingang Fu ,&nbsp;Weiwei Guo ,&nbsp;Yuheng Cheng ,&nbsp;Lin Li","doi":"10.1016/j.chroma.2025.465939","DOIUrl":"10.1016/j.chroma.2025.465939","url":null,"abstract":"<div><div>This study presented the development and validation of a robust ultra-performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS) method for the simultaneous quantification of tryptophan (TRP) and its nine metabolites in aqueous humor (AH) to explore the regulation of the TRP metabolic pathway in primary angle-closure glaucoma (PACG). The optimized UPLC-MS/MS method demonstrated good linearity (R² &gt; 0.99), sensitivity (LLMI: 0.11 – 1.31 ng/mL), precision (CVs: 2.18 % and 12.88 %), recovery rates (85.06 % – 105.74 %), bench-top, long-term and on-instrument stabilities (CVs: 2.35 % – 6.88 %). The validated UPLC-MS/MS method was applied to AH samples from PACG patients with cataract and cataract-alone patients. The results showed that kynurenine and 3-hydroxykynurenine concentrations were significantly increased in the AH of the PACG group, indicating up-regulated indoleamine 2,3-dioxygenase activity and a metabolic shift towards the production of the neurotoxic metabolites within the kynurenine pathway. These findings underscore the potential involvement of TRP metabolism in PACG pathogenesis.</div></div>","PeriodicalId":347,"journal":{"name":"Journal of Chromatography A","volume":"1750 ","pages":"Article 465939"},"PeriodicalIF":3.8,"publicationDate":"2025-04-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143800169","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"De novo design and discovery of broad-spectrum affinity peptide ligands for influenza A vaccines","authors":"Zengquan Tian ,&nbsp;Xiaoyan Dong ,&nbsp;Yan Sun ,&nbsp;Qinghong Shi","doi":"10.1016/j.chroma.2025.465937","DOIUrl":"10.1016/j.chroma.2025.465937","url":null,"abstract":"<div><div>Seasonal Influenza viruses, owing to their continued evolution and high level of contagion, present a significant threat to public health around world each year. Vaccination remains the most effective strategy for preventing complications of influenza virus infection, particularly for vulnerable populations such as elderly individuals, children, and individuals with underlying health conditions. In this study, we described the <em>de novo</em> design for the discovery of affinity ligands targeting the conserved receptor binding site (RBS) of the influenza virus hemagglutinin (HA). Based on three-round of molecular docking, three candidate peptides, pep1, pep3 and pep4, with top-rankings were identified. Molecular dynamic simulation and per-residue decomposition further revealed the different binding mechanisms of the three peptides with HA and the key residue's contribution to the binding. The result of microscale thermophoresis indicated that the three peptides had broad-spectrum affinity for various influenza A strains and, among them, pep1 had the highest binding affinity for HA (<em>K</em>_d = 0.58-0.73 μmol/L). By coupling pep1 onto Sepharose gels, the affinity gel was applied to the evaluation of the chromatographic performance in the purification of HA and influenza A vaccine from mimic egg- and mammalian-based feedstocks. A recovery of 68.3 %-72.2 % at the purity of 95.9 %-97.2 % was obtained in vaccine purification, demonstrating the excellent feature of the peptide ligand. This work provided new insight into the rational design of broad-spectrum affinity peptide targeting HA and the result has potential application in the production of influenza vaccines.</div></div>","PeriodicalId":347,"journal":{"name":"Journal of Chromatography A","volume":"1750 ","pages":"Article 465937"},"PeriodicalIF":3.8,"publicationDate":"2025-04-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143784086","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Green extraction of bioactive flavonoids in Scutellariae Radix using deep eutectic solvents-based ultrasound-assisted matrix solid phase dispersion combined with computer-aided analysis","authors":"Chuanyuan Gao ,&nbsp;Kunze Du ,&nbsp;Jiake Wen ,&nbsp;Haixiang Li ,&nbsp;Wenping Liu ,&nbsp;Ye Zheng ,&nbsp;Min Han ,&nbsp;Xiaoxia Li ,&nbsp;Yanxu Chang","doi":"10.1016/j.chroma.2025.465938","DOIUrl":"10.1016/j.chroma.2025.465938","url":null,"abstract":"<div><div>An efficient and sustainable deep eutectic solvents-based ultrasound-assisted matrix solid phase dispersion (DES-UAMSPD) method was proposed for extracting the bioactive flavonoids in <em>Scutellariae Radix</em> (SR), with molecular sieve (ZSM-5) as dispersant and betaine-levulinic acid (Bet-Lea (1:2, 50 % water content)) as the effective extractant. The interaction mechanism of DES formation and extraction process was comprehensively elucidated by computer simulation techniques such as electrostatic potential (ESP) distributions, independent gradient model based on hirshfeld partition (IGMH) and frontier molecular orbitals theory. The response surface methodology experiments were conducted to optimize the extraction conditions. Under optimal extraction conditions, the total content of five analytes (baicalin, oroxyloside, wogonoside, baicalein and wogonin) reached 182.66 mg g^-1, which was 1.50–2.19 folds higher than conventional extraction solvents. The greenness and environmental friendliness of the proposed method were evaluated through Green Analytical Procedure Index and Analytical Eco-Scale. Additionally, this quantitative method exhibited reliable linearity for the analytes (<em>r</em>² &gt; 0.9997) and excellent recovery (95.4 %-102 %, RSD &lt; 2.72 %). The limits of detection ranged from 0.02 to 0.04 μg mL^-1. Overall, these results highlighted the excellent extraction and quantification capability of the proposed approach, which offered an environmentally friendly strategy for effective extraction of bioactive compounds from natural products.</div></div>","PeriodicalId":347,"journal":{"name":"Journal of Chromatography A","volume":"1751 ","pages":"Article 465938"},"PeriodicalIF":3.8,"publicationDate":"2025-04-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143800518","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Magnetic multi-template Molecularly imprinted polymers for solid-phase extraction of alkaloids in subprostrate sophora","authors":"Tingfan Tang ,&nbsp;Xuesong Li ,&nbsp;Ziwei Xu ,&nbsp;Jiayu Zhao ,&nbsp;Yaqin Zhan ,&nbsp;Yixing Li ,&nbsp;Lijun Li ,&nbsp;Hao Cheng ,&nbsp;Yuhong Tian ,&nbsp;Wenyi Huang ,&nbsp;Jun Feng","doi":"10.1016/j.chroma.2025.465927","DOIUrl":"10.1016/j.chroma.2025.465927","url":null,"abstract":"<div><div>In this study, magnetic multi-template molecularly imprinted polymers (MMIPs) are successfully synthesized. The synthesis is carried out by a multi-template surface imprinting strategy using mesoporous silica-coated magnetic graphene oxide as the support material, matrine (MT), oxymatrine (OMT) and sophocarpine (SC) as the templates, 4-vinylbenzoic acid as the functional monomer, and ethylene glycol dimethacrylate (EDGMA) as the cross-linker. The MMIPs were characterized using Fourier-transform infrared spectroscopy, X-ray diffraction, scanning electron microscopy, and transmission electron microscopy. The maximum adsorption capacity of the prepared MMIPs was found to be 9.01, 20.87, and 7.76 mg/g for MT, OMT, and SC, respectively, demonstrating higher adsorption capacity compared to the non-imprinted polymers (MNIPs). Furthermore, MMIPs were utilized as adsorbents for magnetic solid-phase extraction (MSPE) in conjunction with pressurized capillary electrochromatography (pCEC) for the simultaneous separation and detection of MT, OMT, and SC in the Chinese herbal medicine substrate sophora. The factors affecting the extraction efficiency of MSPE, including the type of elution solvent, amount of adsorbent, pH of the sample solution, elution time, and eluent volume, were optimized. The MMIPs-pCEC method demonstrated a satisfactory linear response within the concentration range of 1–50 µg/mL for the three alkaloids, with correlation coefficients ranging from 0.9923 to 0.9972 and limits of detection ranging from 0.09 to 0.73 µg/mL. The findings of the present study demonstrate that MMIPs have important applications in the analysis of active ingredients in traditional Chinese medicine.</div></div>","PeriodicalId":347,"journal":{"name":"Journal of Chromatography A","volume":"1750 ","pages":"Article 465927"},"PeriodicalIF":3.8,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143784087","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Dispersion properties of triply periodic minimal surface stationary phases for LC: The mesoporous case","authors":"Carolina Lauriola ,&nbsp;Ali Moussa ,&nbsp;Gert Desmet ,&nbsp;Alessandra Adrover","doi":"10.1016/j.chroma.2025.465876","DOIUrl":"10.1016/j.chroma.2025.465876","url":null,"abstract":"<div><div>Eight different 3D printable mesoporous stationary phases based on triply periodic minimal surfaces (TPMS) are investigated. Their separation performances, for different values of the zone retention factor <span><math><msup><mrow><mi>k</mi></mrow><mrow><mo>′</mo><mo>′</mo></mrow></msup></math></span>, are analyzed in terms of plate height curves and kinetic performance factors. The two-zone moment analysis method (TZMA) for the determination of the dispersion tensor in hierarchical retentive porous media has been adopted to compute the dispersion coefficients under retentive conditions. The contribution of the internal porosity of the stationary zone drastically change the chromatographic performance of TPMS structures with respect to the non-retentive (hydrodynamic) and superficially retentive cases. Sheet-based TPMS structures are best performing geometries because the analysis of kinetic plots reveals that optimal functioning conditions are associated with optimal dimensions of the unit cell compatible with printing resolution of cutting-edge 3D printers.</div></div>","PeriodicalId":347,"journal":{"name":"Journal of Chromatography A","volume":"1750 ","pages":"Article 465876"},"PeriodicalIF":3.8,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143777134","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Tertiary amine-functionalized metal-organic frameworks for high adsorption of perfluoroalkyl and polyfluoroalkyl substances in environmental water samples","authors":"Zhuoning Li ,&nbsp;Xiaoli Wang ,&nbsp;Leilei Wang ,&nbsp;Lu Liu ,&nbsp;Lingxi Zhao ,&nbsp;Na Li ,&nbsp;Hailong Jiang ,&nbsp;Zhineng Hao ,&nbsp;Jingfu Liu ,&nbsp;Xiangfeng Chen ,&nbsp;Rusong Zhao","doi":"10.1016/j.chroma.2025.465926","DOIUrl":"10.1016/j.chroma.2025.465926","url":null,"abstract":"<div><div>Sensitive monitoring of per- and polyfluoroalkyl substances (PFASs) is critical for environmental and health risk assessment. In this work, metal-organic frameworks (MOFs) functionalized with positively charged tertiary amines (UiO-67-CH₂-N(CH₃)₂) were synthesized by the one-pot route, which integrated solvothermal MOFs assembly and in-situ reductive amination of formyl groups. The fabricated UiO-67-CH₂-N(CH₃)₂ exhibited exceptional performance as adsorbents for solid-phase extraction (SPE), and the efficient adsorption of PFASs was attribute to the electrostatic attraction and hydrogen bonding. The proposed SPE-HPLC-MS/MS method demonstrated low limit of detection (0.02–0.88 ng·L⁻¹), good linearity (0.2–400 ng·L⁻¹) and high precision (RSD &lt; 8.18 %). The effectiveness of the established method was assessed through the analysis of PFASs in environmental waters, and recoveries in spiked samples were 84.0–117 % with RSD &lt; 13 %. This study highlighted the efficacy of the functionalized UiO-67-CH₂-N(CH₃)₂ in trace PFASs analysis, advancing their application in environmental monitoring.</div></div>","PeriodicalId":347,"journal":{"name":"Journal of Chromatography A","volume":"1750 ","pages":"Article 465926"},"PeriodicalIF":3.8,"publicationDate":"2025-03-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143783029","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Construction of carboxyl-functionalized hyper-cross-linked porous polymers using waste polystyrene for effective adsorption of phenolic contaminants","authors":"Chenhuan Wang ,&nbsp;Qianqian Wang ,&nbsp;Kaiyuan Si","doi":"10.1016/j.chroma.2025.465925","DOIUrl":"10.1016/j.chroma.2025.465925","url":null,"abstract":"<div><div>The extensive presence of phenolic organic contaminants (POCs) poses a serious threat to humans. Meanwhile, the upcycling/reusing of waste polystyrene to reduce the exponential growth of plastic pollution is a very important environmental issue. Addressing these demands, a series of carboxyl-functional hypercrosslinked polymers (labeled PP-HCPs) were constructed by knitting waste polystyrene with pyromellitic dianhydride at different ratios through a one-step Friedel-Crafts reaction for effective adsorption of POCs. Among the prepared PP-HCPs, PP-HCP2 displayed a large specific surface area with high adsorption capacity (37.3 mg g⁻¹) for POCs. Using PP-HCP2 as solid phase extraction sorbent, six POCs were effectively extracted from water and peach drink samples, then subjected to high-performance liquid chromatography-ultraviolet detection. The method demonstrated good linearity in the range of 0.03–100.0 ng mL⁻¹ for water samples and 0.06–100.0 ng mL⁻¹ for peach drinks under optimum experimental conditions. At a signal-to-noise ratio of 3, low detection limits were found to be 0.01–0.10 ng mL⁻¹ for water samples and 0.02–0.15 ng mL⁻¹ for peach drinks. Good accuracy and repeatability were achieved with recoveries of 85.3–111.8 % and the relative standard deviations below 8.6 %. The PP-HCP2-based approach can be employed as a dependable and sensitive tool to detect POCs in water and peach drink samples. This work delivers a simple and economically viable approach to fabricate carboxyl-functional HCPs by converting waste foam into high-value-added sorbent, with great significance for sustainable development.</div></div>","PeriodicalId":347,"journal":{"name":"Journal of Chromatography A","volume":"1750 ","pages":"Article 465925"},"PeriodicalIF":3.8,"publicationDate":"2025-03-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143759218","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Analytical workflow for comprehensive blood metabolomics analysis by GC-MS. Application to children with ventilator associated pneumonia","authors":"M. Ioannidis ,&nbsp;T. Mouskeftara ,&nbsp;E. Iosifidis ,&nbsp;M. Simitsopoulou ,&nbsp;E. Roilides ,&nbsp;H. Gika ,&nbsp;María Fernanda Rey-Stolle ,&nbsp;C. Virgiliou","doi":"10.1016/j.chroma.2025.465924","DOIUrl":"10.1016/j.chroma.2025.465924","url":null,"abstract":"<div><div>Metabolomics is a widely used approach for analyzing a vast array of low molecular weight compounds such as amino acids, organic acids, vitamins, biogenic amines and carbohydrates in biological samples, with the aim of investigating biomarkers in personalized medicine studies. Advancements in gas chromatography- mass spectrometry (GC–MS) instrumentation, along with the availability of commercial and public spectral libraries, have highlighted the relevance of GC–MS analysis as a valuable tool for metabolomics applications. Stability assessment in derivatisation and GC–MS analysis is a crucial yet often overlooked aspect of metabolomics studies. In this study, an untargeted GC-MS method workflow for large scale metabolomics studies is presented after assessment and optimization of whole blood sample's stability. The method consists of a common two-step derivatisation procedure including methoximation using methoxyamine hydrochloride, followed by silylation with N-methyl-N-(trimethylsilyl) trifluoroacetamide (MSTFA). To ensure the stability of the studied metabolites, extensive stability experiments were performed. The stability of the derivatives was evaluated over 24 h in the autosampler at room temperature, as well as after storage for 24 and 48 h at -20 °C for both derivatized and dried extracts. While derivatised samples remained stable for 24–48 h in the freezer, dried extracts exhibited variability after 48 h. Findings support the storage of derivatised samples over dried extracts, ensuring greater stability. To increase condidence in metabolite identification data from the analysis of 120 standard compounds were utilized. The developed method was applied to analyze blood samples from 32 children with ventilator-associated pneumonia (VAP), collected at four different time points during ICU hospitalization. This analysis led to the identification of 43 metabolites. The results of multivariate and univariate statistical analyses demonstrated several statistically significant metabolites, including aspartic acid, alanine, and pyroglutamic acid, which showed a strong correlation with the disease's manifestation and may potentially serve as biomarkers in the diagnosis of ventilator-associated pneumonia VAP at the stage of clinical suspicion.</div></div>","PeriodicalId":347,"journal":{"name":"Journal of Chromatography A","volume":"1753 ","pages":"Article 465924"},"PeriodicalIF":3.8,"publicationDate":"2025-03-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143886690","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Investigating the binding products between human apolipoproteins and oxidized 1-palmitoyl-2-arachidonyl-sn-glycerol-3-phosphocholine by micellar electrokinetic chromatography","authors":"Zhi-Cheng Ye ,&nbsp;Jia-Yuan Liu ,&nbsp;Chao-Jung Chen ,&nbsp;Yen-Yi Chen ,&nbsp;Wen-Chun Li ,&nbsp;Mine-Yine Liu","doi":"10.1016/j.chroma.2025.465898","DOIUrl":"10.1016/j.chroma.2025.465898","url":null,"abstract":"<div><div>A micellar electrokinetic chromatography (MEKC) method has been developed to investigate the binding products between human apolipoproteins (Apos) and oxidized 1-palmitoyl-2-arachidonoyl-sn‑glycero-3-phosphocholine (ox-PAPC) products. The optimal MEKC separation buffer was composed of a solution mixture of 10 mM sodium phosphate, 50 mM bile salts (50 % sodium cholate and 50 % sodium deoxycholate), 30 % (v/v)1-propanol and 70 % (v/v) water, pH 7.4. The optimal MEKC sample buffer was composed of 70 % (v/v) PBS buffer and 30 % (v/v) MeOH. The selected separation voltage was 20 kV, and the capillary temperature was 25℃. The MEKC profiles of ox-PAPC products showed good separations and repeatability. The MEKC profiles of apos and their binding products also showed good repeatability.</div><div>For the analysis of native PAPC (n-PAPC), the method is linear in the range of 0.00–6.00 mg/mL with a correlation coefficient 0.9984. The concentration limit of detection (LOD) is 0.29 mg/mL. The concentration limit of quantitation (LOQ) is 0.98 mg/mL.</div><div>The binding reactions between several important human apolipoproteins (Apos A-I, A-II, C-I, C-II, C-III and E) and native PAPC, ox-PAPC products have been investigated. The concentrations of Apos and ox-PAPC products for binding reactions have been examined. The optimal binding buffer selected was 70 % (v/v) PBS buffer and 30 % (v/v) MeOH. The binding reaction was performed at 37 ℃ for 3 hr.</div><div>The results indicated that ox-PAPC products bound to Apos A-I, A-II, C-I, and E more strongly than n-PAPC. However, both ox-PAPC products and n-PAPC did not bind to Apos C-II and C-III strongly. The results suggested pro-inflammatory properties of Apos A-I, A-II, C-I, and E, and implied one of the molecular mechanisms resulting in dysfunctional HDL particles. This study also demonstrated the feasibility of investigating the binding reactions between human apolipoproteins and ox-PAPC products by MEKC.</div></div>","PeriodicalId":347,"journal":{"name":"Journal of Chromatography A","volume":"1751 ","pages":"Article 465898"},"PeriodicalIF":3.8,"publicationDate":"2025-03-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143821007","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A dual-polarity ion mobility spectrometer for capturing hyper-fast chromatographic peaks","authors":"Alexander Nitschke,&nbsp;Moritz Hitzemann,&nbsp;Jonas Winkelholz,&nbsp;Ansgar T. Kirk,&nbsp;Christoph Schaefer,&nbsp;Tim Kobelt,&nbsp;Christian Thoben,&nbsp;Martin Lippmann,&nbsp;Jan A. Wittwer,&nbsp;Stefan Zimmermann","doi":"10.1016/j.chroma.2025.465923","DOIUrl":"10.1016/j.chroma.2025.465923","url":null,"abstract":"<div><div>For higher analytical performance, ion mobility spectrometers (IMS), especially when analyzing complex mixtures, are often coupled with gas chromatographs (GC). However, analysis time remains a critical parameter in gas chromatography. Therefore, a hyper-fast GC is a promising approach for GC-IMS to combine highest sensitivity of IMS with increased peak capacity while allowing for rapid analysis within the double-digit second range. For simultaneously recording of both ion polarities, a new dual drift tube IMS in axial configuration has been developed that is particularly designed for resolving even hyper-fast GC peaks. Key is a directed sample gas flow through the ionization region giving a small effective detector volume. To avoid condensation of the sample gas, the IMS is isothermally heated to 120 °C. A drift tube length of only <em>L_D</em> = 41 mm with a drift voltage of <em>U_D</em> = 2.7 kV leads to an IMS repetition rate of 100 Hz and high resolving power of <em>R_p</em> = 60, even for ions with low reduced ion mobility down to 0.43 cm²V^-1s^-1. This setup can resolve very short GC peaks with full width at half maximum of only 140 ms. For demonstration, two different hop varieties and a mixture of explosives were analyzed within a short time of just 20 s.</div></div>","PeriodicalId":347,"journal":{"name":"Journal of Chromatography A","volume":"1750 ","pages":"Article 465923"},"PeriodicalIF":3.8,"publicationDate":"2025-03-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143759298","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}