Journal of Chromatography B最新文献

{"title":"Green RP-HPLC method for the estimation of carfilzomib in bulk, protein nanocarriers and human plasma: Application of chemometrics and Monte-Carlo simulations","authors":"Drishti Panjwani ,&nbsp;Asha Patel ,&nbsp;Deepak Mishra ,&nbsp;Shruti Patel ,&nbsp;Viral Patel ,&nbsp;Mange Ram Yadav ,&nbsp;Bhupinder Singh","doi":"10.1016/j.jchromb.2024.124350","DOIUrl":"10.1016/j.jchromb.2024.124350","url":null,"abstract":"<div><div>Carfilzomib is a tetrapeptide epoxyketone that has shown potential clinical outcomes in the treatment of multiple myeloma. However, inaccuracies in quantifying such peptide drug products have arisen due to poor stability, low solubility, time-consuming techniques, complex physicochemical properties, and use of non-green solvents with less recyclability. This provides a substantial urge to develop an ecological and sensitive analytical method for quantifying peptide drugs from matrix formulation and biological samples in early as well as lateral stages of product development in pharma industries. As a result, the study aimed to develop a robust ecological method for estimation of carfilzomib via Green RP-HPLC using analytical quality by design (AQbD) paradigms with specific application in protein nanoparticles and biological matrix. Initially, an appropriate wavelength for quantification of carfilzomib was chosen using principal component analysis (PCA) as a chemometric tool.Risk assessment followed by factor screening studies using 8-factor Placket-Burman Design aided in earmarking critical method parameters (CMPs) affecting critical analytical attributes (CAAs). Further, Central Composite Design (CCD) was employed for design space optimisation to demarcate optimum chromatographic conditions, which were corroborated for robustness using Monte-Carlo simulations. The method was validated as per ICH Q2 (R2), followed by quantifying the greenness of the method using Green Assessment tools. The method optimisation resulted in the optimal chromatographic conditions using Green RP-HPLC. The chromatographic system was equipped with a Phenomenex Aeris Peptide-XC C₁₈ column (150 × 4.6 mm × 5 µm), and the mobile phase was composed of isopropanol:methanol:0.1 M PBS (pH 5.5 adjusted using 0.1 % formic acid) (35:45:20v/v), with a 1 ml/min flow rate at a 210 nm ʎmax. The optimised chromatographic conditions resulted in a short retention time (RT) of 4.95 mins, 0.87 tailing factor (TF), 4,875,122 peak area (PA), and 8995 theoretical plate count (TPC). The method demonstrated linearity in a wide range of concentrations (0.1–20 µg/ml) with a correlational coefficient of 0.997 and &lt; 2 % RSD. The method unearthed a high precision rate with more than 95 % of drug recovery in protein nanoparticles and human plasma, thereby confirming the accuracy and sensitivity of the developed method. Chemometrics and Monte-Carlo simulations ratified the robustness and sensitivity of the developed analytical method of Carfilzomib with established greenness and a high degree of practical utility in protein-based nano formulations and human plasma matrix for life cycle product development.</div></div>","PeriodicalId":348,"journal":{"name":"Journal of Chromatography B","volume":"1249 ","pages":"Article 124350"},"PeriodicalIF":2.8,"publicationDate":"2024-11-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142664033","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Methylated magnetic covalent organic framework for sample preparation and LC-MS/MS detection of 12 tadalafil analogs in dietary supplements","authors":"Meng Li ,&nbsp;Xiuli Xu ,&nbsp;Xiujuan Wang ,&nbsp;Feng Feng ,&nbsp;Jie Lian ,&nbsp;Feng Zhang","doi":"10.1016/j.jchromb.2024.124341","DOIUrl":"10.1016/j.jchromb.2024.124341","url":null,"abstract":"<div><div>Tadalafil analogs are often illegally added to dietary supplements such as herbal beverages, protein powders and tablet foods. Due to the complexity of the matrices, effective extraction of tadalafil analogs is the key to achieve accurate quantification. Therefore, it is of great significance to establish a rapid and effective method for the analytical determination of tadalafil analogs in complex matrices. In this study, a novel methylated magnetic covalent organic framework, Fe₃O₄@TFPB-OT, was successfully synthesized under mild conditions. Fe₃O₄@TFPB-OT demonstrated robust adsorption capabilities, with capacities ranging from 52.4 to 90.9 mg/g for the tadalafil analogs. Several pre-enrichment parameters were optimized, including adsorbent dosage, extraction time, pH, shaking time, elution solvent, and desorption time. The applicability of Fe₃O₄@TFPB-OT was evaluated as effective adsorbents for the magnetic solid-phase extraction (MSPE) of 12 tadalafil analogs in dietary supplements. Combined with high-performance liquid chromatography-tandem mass spectrometry, the limits of detection (LODs) of this method ranged from 0.005 to 0.05 μg/L in liquid matrices and from 0.005 to 0.05 μg/kg in solid matrices, showing good sensitivity and recoveries ranged from 56.1 % to 90.9 %with relative standard deviations lower than 3.9 %, demonstrating good accuracy and precision. Additionally, the adsorbent retained its effectiveness after at least ten reuse cycles, indicating significant reusability. This study provides an effective method for the analysis and detection of tadalafil analogs in dietary supplements and has great potential for application.</div></div>","PeriodicalId":348,"journal":{"name":"Journal of Chromatography B","volume":"1249 ","pages":"Article 124341"},"PeriodicalIF":2.8,"publicationDate":"2024-11-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142646700","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Progress in the technology of solvent flotation","authors":"Na Li ,&nbsp;Yuchi Zhang ,&nbsp;Mengyao Gao ,&nbsp;Chen Yan ,&nbsp;Yun Wei","doi":"10.1016/j.jchromb.2024.124370","DOIUrl":"10.1016/j.jchromb.2024.124370","url":null,"abstract":"<div><div>Solvent flotation primarily relies on the variations in the activity of substances to adsorb target compounds onto the surface of bubbles, thereby facilitating the process of separation and extraction. This technology has the advantages of high separation efficiency, gentle process, and simple operation, making it widely applicable across various fields. This article reviews relevant research from the past decade to analyze the factors influencing this technology. Additionally, it provides a comprehensive overview of its applications in detecting organic matter in environmental samples and extracting bioactive compounds from natural products, while also anticipating upcoming trends in its development.</div></div>","PeriodicalId":348,"journal":{"name":"Journal of Chromatography B","volume":"1249 ","pages":"Article 124370"},"PeriodicalIF":2.8,"publicationDate":"2024-11-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142646701","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Determination of furmonertinib in human plasma and cerebrospinal fluid by UPLC-MS/MS: Application in lung cancer patients with and without brain metastasis","authors":"Hongxin Qie ,&nbsp;Cong Song ,&nbsp;Yuxiang Xu ,&nbsp;Haopeng Zhao ,&nbsp;Wenlin Gong ,&nbsp;Peiyuan Wang ,&nbsp;Xiaonan Gao ,&nbsp;Jinglin Gao ,&nbsp;Zhangying Feng ,&nbsp;Mingxia Wang","doi":"10.1016/j.jchromb.2024.124375","DOIUrl":"10.1016/j.jchromb.2024.124375","url":null,"abstract":"<div><div>Furmonertinib (AST2818) is a selective epidermal growth factor receptor (EGFR) tyrosine kinase inhibitor (TKI) being developed for the treatment of patients with EGFR mutation-positive non-small cell lung cancer. Quantification of furmonertinib in plasma and cerebrospinal fluid (CSF) can be used to assess penetration of furmonertinib into the central nervous system (CNS). This paper described ultra-performance liquid chromatography tandem mass spectrometry (UPLC-MS/MS) methods for quantification of furmonertinib in human plasma and CSF. Sample separation was achieved on a Kinetex C₁₈ column (100 mm × 2.1 mm, 2.6 μm) after simple protein precipitation with acetonitrile. The mobile phase was composed of acetonitrile and 5 mM ammonium acetate with 0.2 % formic acid in water. Quantitative ion pairs were <em>m</em>/<em>z</em> 569.3 → 72.2 for furmonertinib and <em>m</em>/<em>z</em> 526.5 → 72.2 for aumolertinib, which was used as the internal standard (IS). The calibration curves showed good linearity (r² &gt; 0.99) over concentration range of 0.5–200 ng/mL(plasma sample) and 0.05–30 ng/mL(CSF sample). The precision (RSD) was ≤7.86 %, and the accuracy fell within the range of 96.2 %–109.3 %, all meeting acceptance criteria. The matrix effect was from 94.3 % to 102.1 %. The recovery of analytes fell within the range of 93.3 %–98.9 %. The established analytical methods showed great sensitivity, simplicity, accuracy and reliability for the analysis of furmonertinib in human plasma and CSF. This assay would be helpful to predict the effectiveness and toxicities of furmonertinib in the pursuit of precision medicine for lung cancer patients.</div></div>","PeriodicalId":348,"journal":{"name":"Journal of Chromatography B","volume":"1248 ","pages":"Article 124375"},"PeriodicalIF":2.8,"publicationDate":"2024-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142643553","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Extension of impurity profiling on eltrombopag olamine to in-silico predictions: An effort to exploit correlated forced degradation products and known drug-related substances in drug discovery","authors":"Saurabh B. Ganorkar ,&nbsp;Preeti S. Bobade ,&nbsp;Rakesh C. Prabhu ,&nbsp;Deepak K. Lokwani ,&nbsp;Ranajit N. Shinde ,&nbsp;Darshan R. Telange ,&nbsp;Atul A. Shirkhedkar ,&nbsp;Yvan Vander Heyden","doi":"10.1016/j.jchromb.2024.124367","DOIUrl":"10.1016/j.jchromb.2024.124367","url":null,"abstract":"<div><div>The recent pandemic has highlighted the impact of diseases on global health and the economy. The rapid discovery of new hit molecules remains a tough challenge. Pharmaceutical impurity profiling can be linked to drug discovery through the identification of new hits from compounds identified during the analytical profiling. The present study demonstrates this linkage through the extension of the impurity (forced degradation) profiling of eltrombopag (ELT) olamine, a thrombopoietin (TPO) receptor agonist. The drug was exposed to standard degradation and the degradation products were primarily resolved and identified by UPLC-ESI-MS. This led to the identification of five forced degradation products (FDP). Thirty-three other known related substances (RS) of ELT, identified in the literature, were also considered. Molecular similarity checks were performed using Tanimoto/Jaccard's similarity searches. A set of structurally and topologically similar molecules, including ELT and 15 RS, was established and subjected to in-silico toxicity-, absorption-, distribution-, metabolism-, and elimination (ADME) predictions. The RS, predicted with similar or lower toxicity than ELT and a comparable ADME profile, were subjected to molecular docking to trace changes in TPO receptor affinity. The results indicated that five RS had a high Jaccard’s similarity with ELT and higher or comparable docking scores. These compounds, along with few other impurities were predicted to have lower toxicity, better or comparable absorption, distribution, metabolism, and also a better excretion profile than ELT. This justifies their entry as potential novel TPO receptor agonists in drug discovery.</div></div>","PeriodicalId":348,"journal":{"name":"Journal of Chromatography B","volume":"1248 ","pages":"Article 124367"},"PeriodicalIF":2.8,"publicationDate":"2024-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142638171","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Pharmacokinetic profiling and network pharmacology of honey-fried Licorice: An Integrative workflow to study traditional Chinese medicines (TCMs)","authors":"Lifeng Zhao ,&nbsp;Xin Yu ,&nbsp;Siyang Wu ,&nbsp;Kexin Xia ,&nbsp;Yuyan Wang ,&nbsp;Peichong Qin ,&nbsp;Zhishan Huang ,&nbsp;Chen Kang ,&nbsp;Zheng Yuan ,&nbsp;Yingfei Li","doi":"10.1016/j.jchromb.2024.124353","DOIUrl":"10.1016/j.jchromb.2024.124353","url":null,"abstract":"<div><div>Licorice, known as the “elder statesman,” is commonly used in traditional Chinese medicine (TCM) formulations. This study aims to establish a workflow combining animal and in silico experiments to elucidate the mechanisms of TCMs at both qualitative and quantitative levels. UPLC-Q-TOF-MS/MS was employed to qualitatively characterize the total components of honey-fried licorice and the plasma components after oral administration in Beagle dogs. A UPLC-Q-Trap-MS/MS method was developed for the pharmacokinetic study of honey-fried licorice components in Beagle dog plasma. Network pharmacology and molecular docking were utilized to explore the primary functional targets and pathways. In total, we identified 68 constituents in honey-fried licorice, with 28 detected in Beagle dog plasma, and 18 of them, mainly belong to flavonoids and terpenoids, showing significant exposure. The plasma pharmacokinetic study of these 18 constituents revealed that compounds like liquiritin, glycyrrhizic acid, licoricesaponin G2, and glycyrrhetic acid-3-o-glucuronide had significant exposure. Network pharmacology and molecular docking analyses identified MAPK3, PIK3CB, PIK3CA, RAF1, and EGFR as the main targets of the active constituents of honey-fried licorice, involved in pathways such as the Ras signaling pathway, human cytomegalovirus infection, and the MAPK signaling pathway. This study provides a comprehensive profile and pharmacokinetic characteristics of honey-fried licorice, offering insights into its pharmacological, toxicological, and clinical aspects. The established workflow can serve as a standard for investigating other TCMs.</div></div>","PeriodicalId":348,"journal":{"name":"Journal of Chromatography B","volume":"1248 ","pages":"Article 124353"},"PeriodicalIF":2.8,"publicationDate":"2024-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142611269","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Deciphering the impact and mechanism of total flavonoids from Cortex Juglandis Mandshuricae on alcoholic fatty liver employing LC-MS/MS, network pharmacology analysis and in vitro validation","authors":"Tianmei Niu ,&nbsp;Jiaxin Wang ,&nbsp;Liying Xun ,&nbsp;Bingqing Zheng ,&nbsp;Zhipeng Deng ,&nbsp;Zhi Chen ,&nbsp;Kaijie Jia ,&nbsp;Pan Zhao ,&nbsp;Qitao Zhao","doi":"10.1016/j.jchromb.2024.124334","DOIUrl":"10.1016/j.jchromb.2024.124334","url":null,"abstract":"<div><div>The Cortex Juglandis Mandshuricae (CJM) has the efficacy of penetrating the liver meridian, removing heat and dampness, and alleviating the liver, which corresponds to the pathogenesis of alcoholic fatty liver disease (AFLD) with damp heat accumulation. Modern research has shown that total flavonoids from Cortex Juglandis Mandshuricae (TFC) have hepatoprotective, antioxidant and antitumour pharmacological effects. However, there is no any investigation on the mechanism of TFC improving AFLD. In this work, a valid strategy combining UPLC-Q-Exactive Orbitrap-MS, network pharmacology and in vitro cellular experimental validation is proposed to predict the targets and pathways of TFC to ameliorate AFLD and to explore its mechanism of action. As a result, 26 flavonoids and 182 targets linked to TFC and AFLD were identified. These compounds realize their critical targets via various signaling pathways and perform multiple biological functions on the basis of the constructed compound-disease target networks. In vitro experiments demonstrated TFC had a protective impact on ethanol-treated L02 cells to a certain extent and could diminished lipid accretion. In addition, RT-qPCR and western blot results illustrated that TFC could regulate the expression of PPARα, CPT-1, SREBP-1c and FAS, and inhibit alcohol-induced lipid accumulation in L02 cells thereby alleviating AFLD. The present study further provides experimental justification for TFC to ameliorate AFLD in practical applications.</div></div>","PeriodicalId":348,"journal":{"name":"Journal of Chromatography B","volume":"1248 ","pages":"Article 124334"},"PeriodicalIF":2.8,"publicationDate":"2024-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142602251","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Evaluation of the ionization efficiency in phosphatidylcholine positional isomers with docosahexaenoic acid bound to the sn-1 or sn-2 position","authors":"Kana Fujiwara ,&nbsp;Seiya Tanaka ,&nbsp;Koyama Tomoyuki ,&nbsp;Kazuaki Yoshinaga ,&nbsp;Naohiro Gotoh","doi":"10.1016/j.jchromb.2024.124355","DOIUrl":"10.1016/j.jchromb.2024.124355","url":null,"abstract":"<div><div>Phosphatidylcholine (PC), a key phospholipid, contains 2 fatty acids that can be bound at the sn-1 and sn-2 positions, resulting in positional isomers when different fatty acids are attached. Currently, there is no established method for identifying phospholipid molecular species and quantifying individual isomers using authentic standards of each PC isomer. In this study, we prepare authentic analytical standards for PC positional isomers through chemical synthesis and preparative purification. These isomers contain docosahexaenoic acid (DHA, 22:6) and palmitic acid (16:0) attached at the sn-1 and sn-2 positions and are denoted as PC(22:6/16:0) and PC(16:0/22:6), respectively. Standard solutions of PC(22:6/16:0) and PC(16:0/22:6) were analyzed using liquid chromatography-tandem mass spectrometry, and calibration curves of the PC positional isomers were generated to compare their ionization efficiencies. The ionization efficiency of PC(22:6/16:0) was 2.32 times higher than that of PC(16:0/22:6), indicating that the ionization efficiency depends on the binding position of the fatty acid. Elucidating and correcting the differences in the ionization efficiencies of the PC positional isomers will enable the accurate quantitative analysis of lipidomes in the future.</div></div>","PeriodicalId":348,"journal":{"name":"Journal of Chromatography B","volume":"1248 ","pages":"Article 124355"},"PeriodicalIF":2.8,"publicationDate":"2024-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142643556","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Exploring the pharmacological mechanism of fermented Eucommia ulmoides leaf extract in the treatment of cisplatin-induced kidney injury in mice: Integrated traditional pharmacology, metabolomics and network pharmacology","authors":"Kexin Lin ,&nbsp;Lijuan Xiong ,&nbsp;Wen Zhang ,&nbsp;Xuan Chen ,&nbsp;Jieqi Zhu ,&nbsp;Xiaofei Li ,&nbsp;Jianyong Zhang","doi":"10.1016/j.jchromb.2024.124358","DOIUrl":"10.1016/j.jchromb.2024.124358","url":null,"abstract":"<div><div>Cisplatin (CP) is a widely utilized anticancer drug, which also produces significant side effects, notably acute kidney injury (AKI). Fermented <em>Eucommia ulmoides</em> leaf (FEUL), a medicinal and edible Chinese herbal remedy, is known for its renoprotective properties. However, the effect and underlying mechanism of FEUL extract in AKI therapy have remained largely unexplored. This research aimed to elucidate the protective roles of FEUL extract in an AKI mouse model through biochemical assays, histopathological examinations, and investigating the underlying mechanisms based on metabolomics and network pharmacology. The findings demonstrated that pretreatment with orally administered FEUL extract significantly reduced blood urea nitrogen (BUN), and serum creatinine (SCr) levels, and ameliorated CP-induced kidney histopathological injuries. Moreover, FEUL extract attenuated CP-induced endoplasmic reticulum (ER) stress by reducing the protein expressions of PERK, IRE 1α, GRP78, ATF6, ATF4, and CHOP. The metabolomics results indicated that a total of 31 metabolites, involved in taurine and hypotaurine metabolism, lysine degradation, and steroid hormone biosynthesis, were altered after FEUL extract administration. Furthermore, metabolomics integrated with network pharmacology revealed that 8 targets, 4 metabolites, and 3 key pathways including steroid hormone biosynthesis, purine metabolism, and tryptophan metabolism were the main mechanisms of FEUL extract in treating CP-induced AKI. These findings suggested that FEUL extract could offer valuable insights for potential CP-induced AKI treatment strategies.</div></div>","PeriodicalId":348,"journal":{"name":"Journal of Chromatography B","volume":"1248 ","pages":"Article 124358"},"PeriodicalIF":2.8,"publicationDate":"2024-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142611284","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Spectrum-effect relationship between HPLC fingerprints and antioxidant activities of Bletilla striata","authors":"Sha Wen,&nbsp;Yuzhi Luo,&nbsp;Lingyi Liu,&nbsp;Lili Zhou,&nbsp;Lingli Li,&nbsp;Siqi Wang,&nbsp;Huixin Song,&nbsp;Songyuan Xia,&nbsp;Weifeng Li,&nbsp;Xiaofeng Niu","doi":"10.1016/j.jchromb.2024.124351","DOIUrl":"10.1016/j.jchromb.2024.124351","url":null,"abstract":"<div><div><em>Bletilla striata</em> is a perennial herb that was first published in Shennong’s Classic of the Materia Medica, pharmacological studies have shown that it has the activities of promoting wound healing, anti-inflammatory and antioxidant. However, the relationship between the antioxidant activity and the chemical composition of <em>Bletilla striata</em> is still unclear. In this paper, the chemometric method was used to construct the spectral effect relationship between the fingerprints of 20 batches of <em>Bletilla striata</em> extracts from different origins and their in vitro antioxidant activities. The results showed that the chemical composition of the samples from different sources varied significantly, while the samples from Shaanxi and Hubei provinces were of relatively better quality. Among the 10 common peaks, coelonin, gymnoside IX and dactylorhin A were considered to be significantly correlated with the antioxidant activity of <em>Bletilla striata</em>. The results of this study will provide a basis and further insights for the quality evaluation and quality control of <em>Bletilla striata.</em></div></div>","PeriodicalId":348,"journal":{"name":"Journal of Chromatography B","volume":"1248 ","pages":"Article 124351"},"PeriodicalIF":2.8,"publicationDate":"2024-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142638172","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}