化学•材料最新文献

{"title":"All-in-one microfluidic immunosensing device for rapid and end-to-end determination of salivary biomarkers of cardiovascular diseases.","authors":"Baihui Liang, Shan Wang, Junmei Zheng, Bin Li, Ningtao Cheng, Ning Gan","doi":"10.1016/j.bios.2024.117077","DOIUrl":"10.1016/j.bios.2024.117077","url":null,"abstract":"<p><p>Routine screening for cardiovascular diseases (CVDs) through point-of-care assays for at-home or community-based testing of salivary biomarkers can significantly improve patient outcomes. However, its translatability has been hindered by a dearth of biosensing devices that streamline assay procedures for rapid biomarker quantitation. To address this challenge through end-to-end engineering, we developed an in-house, all-in-one microfluidic immunosensing device that integrates on-chip vibration-enhanced incubation, magnetic-assisted separation using immune magnetic bead probes, and colorimetric readout via absorbance measurements. This device enables probe preparation and one-pot immunoassay procedures on a reusable microfluidic chip. By engaging the vibrator with the reaction chamber, the vibration-enhanced incubation module significantly accelerates immune complex formation, drastically reducing the sample-to-answer timeline of approximately 1 h required for room temperature enzyme-linked immunosorbent assay (ELISA) to just under 15 min. We showcase the utility of the device with an on-demand assay for a biomarker panel comprising C-reactive protein (CRP), interleukin 6 (IL-6), and procalcitonin (PCT). The device achieved a linear detection range of 1.75-28 ng mL^-1 for CRP and 1.56-100 ng mL^-1 for IL-6 and PCT with an R² > 0.98 for all three biomarkers. The limits of detection were 0.295, 0.400, and 0.947 ng mL^-1, respectively. Results from real saliva samples were consistent with standard ELISA (R² = 0.952). This fully integrated, modular immunosensing device opens up opportunities for household CVD screening and could be adapted for rapid, affordable multiplexed biosensing for other major chronic diseases at the point of care.</p>","PeriodicalId":259,"journal":{"name":"Biosensors and Bioelectronics","volume":"271 ","pages":"117077"},"PeriodicalIF":10.7,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142890780","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Development of a dual-mode lateral flow assay based on structure-guided aptamers for the detection of capsaicin in gutter oils.","authors":"Mingwei Qin, Ning Ding, Pengfei Ma, Hongtao Jiang, Yu Li, Peifang Chen, Zhouping Wang, Junsong Yang","doi":"10.1016/j.bios.2024.117100","DOIUrl":"10.1016/j.bios.2024.117100","url":null,"abstract":"<p><p>The construction of structure-guided aptamers and the ultra-sensitive aptamer-based lateral flow assays (Apt-LFA) integrated detection method hold significant potential for food analysis. Using an engineered modified sequence strategy, we successfully developed the aptamer Cap-1-2M4, significantly enhancing its affinity for capsaicin (CAP) to 0.6197 ± 0.0689 nM. We developed a highly sensitive method for CAP detection based on Vap@AuNFs, leveraging their superior colorimetric and photothermal signals and high aptamer coupling performance. The Vap@AuNFs-based Apt-LFA (Vap@AuNFs-Apt-LFA) achieve signal amplification through catalytic hairpin assembly (CHA) and exonuclease III (Exo III). Additionally, dual-mode (colorimetric and photothermal) quantitative detection via lateral flow test strips was achieved by capturing the nucleic acid effect of Vap@AuNFs. The limit of detection (LOD) of Vap@AuNFs-Apt-LFA by colorimetric/photothermal method was 0.043 ng mL^-1 and 0.011 ng mL^-1, with linear ranges of 0.1-500 ng mL^-1 and 0.05-500 ng mL^-1, respectively. This aptamer engineering strategy and the developed Vap@AuNFs-Apt-LFA offer new directions for food safety and provide a reference for feasible portable detection methods.</p>","PeriodicalId":259,"journal":{"name":"Biosensors and Bioelectronics","volume":"271 ","pages":"117100"},"PeriodicalIF":10.7,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142890788","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Digital microfluidics with integrated Raman sensor for high-sensitivity in-situ bioanalysis.","authors":"Wenbo Dong, Rongxin Fu, Nan Zhang, Jing Zhao, Yudan Ma, Han Cui, Jiangjiang Zhang, Zipeng Zhao, Hang Li, Yunxia Zhao, Yao Lu, Zhizhong Chen, Tianming Xu, Huikai Xie, Qian Yu, Shuailong Zhang","doi":"10.1016/j.bios.2024.117036","DOIUrl":"10.1016/j.bios.2024.117036","url":null,"abstract":"<p><p>This study introduces an advanced bioanalytical platform that combines digital microfluidics (DMF) with Raman spectroscopy, effectively addressing common issues in bioanalysis such as sample contamination, excessive consumption of samples and reagents, and manual handling. Our innovative system is engineered to handle diverse sample types and enables both sample preparation and in-situ analysis on a single device, utilizing less than 5 μL of samples and reagents. It incorporates a Translucent Raman Enhancement Stack (TRES) sensor, which boosts the detection signal, and includes droplet-driving functionality for automated processing of complex samples in a compact setting. The hydrophilic surface of the TRES sensor draws analytes into the detection zone, expediting sample attachment and enhancing the surface-enhanced Raman scattering (SERS). Moreover, the TRES sensors are cost-effective, straightforward to manufacture, and scalable, making them suitable for widespread production and single-use bioanalytical applications. This integrated and automated approach streamlines the sample preparation and analysis processes, enhances detection efficiency, and achieves high sensitivity, excellent linearity, and effective detection of different biochemical analytes. We validate the system's accuracy through on-device enrichment and analysis of exosomes from serum, demonstrating its capability for real-time, on-site analysis of complex biological samples.</p>","PeriodicalId":259,"journal":{"name":"Biosensors and Bioelectronics","volume":"271 ","pages":"117036"},"PeriodicalIF":10.7,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142805581","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Dynamic and quantitative assessment of quercetin for cardiac oxidative stress injury prevention using sensitive cardiomyocyte based biosensing.","authors":"Jie Chen, Xuelian Lyu, Qunchen Yuan, Chunlian Qin, Han Yu, Dongxin Xu, Jilin Zheng, Hongchun Li, Jiaru Fang, Ning Hu, Yuqun Cai","doi":"10.1016/j.bios.2024.117045","DOIUrl":"10.1016/j.bios.2024.117045","url":null,"abstract":"<p><p>Myocardial infarction is a leading cause of morbidity and mortality associated with cardiovascular diseases worldwide. Although novel medications and treatments greatly alleviate patient suffering, challenges related to prognostic limit the recovery of cardiac function. Currently, treatment with monomeric compounds displays promise in prognostic interventions for cardiac diseases. However, there is a lack of dynamic and quantitative assessment of cardiomyocyte response to these drugs. Herein, an integrated biosensing platform with a microelectrode array was constructed for label-free, non-invasive, long-term, and real-time recording of cardiomyocyte electrophysiological signals. By analyzing the signals of cardiomyocytes before and after treatment, we established the safe concentration of quercetin in cardiomyocytes and identified its long-term cardiotoxicity. Moreover, quercetin also demonstrated significant protective effects on cardiomyocytes in a H₂O₂-induced oxidative stress injury model. This study provides a trustworthy platform to evaluate the effects of monomeric compounds on cardiomyocytes, and offers a novel approach for drug screening and efficacy testing in cardiovascular diseases.</p>","PeriodicalId":259,"journal":{"name":"Biosensors and Bioelectronics","volume":"271 ","pages":"117045"},"PeriodicalIF":10.7,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142805586","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Quencher-free CRISPR-based molecular detection using an amphiphilic DNA fluorescence probe.","authors":"Rui Yang, Xin Guan, Jiongyu Zhang, Jeong Moon, Chong Guo, Zhengyang Jia, Chengyu Hou, Uyanga Ganbaatar, Steven Tricarico, Anson W K Ma, Changchun Liu","doi":"10.1016/j.bios.2024.117054","DOIUrl":"10.1016/j.bios.2024.117054","url":null,"abstract":"<p><p>Rapid, sensitive, and specific nucleic acid detection methods play crucial roles in clinical diagnostics and healthcare. Here, we report a novel amphiphilic DNA fluorescence probe for CRISPR-based nucleic acid detection. Unlike conventional fluorophore-quencher probe detection system, our amphiphilic DNA fluorescence probe features a hydrophobic Cy5 fluorophore head and a hydrophilic single-stranded DNA (ssDNA) tail. By combining the amphiphilic DNA fluorescence probe with a paper-based microfluidic device, we developed a quencher-free, CRISPR-based detection system for target nucleic acid quantification. In the presence of the target nucleic acid, the activated CRISPR-Cas12a enzyme cleaves the hydrophilic ssDNA tail of the amphiphilic DNA fluorescence probe, releasing the hydrophobic Cy5 head and altering the wettability of the CRISPR reaction solution. When the CRISPR reaction product is applied to the paper-based microfluidic device, the migration of the cleaved Cy5 head along the hydrophilic microfluidic channel is limited. The higher the target nucleic acid concentration, the shorter the fluorescence diffusion distance, enabling visual quantification of the nucleic acid target. We used human papillomavirus-16 (HPV-16) DNA as a model to evaluate the analytical performance of the system. Furthermore, we validated its clinical feasibility by testing clinical swab samples, achieving results comparable to the traditional PCR method. Our quencher-free CRISPR-based detection system shows potential for simple, affordable, and sensitive clinical diagnostics of HPV-associated cancer and other infectious diseases.</p>","PeriodicalId":259,"journal":{"name":"Biosensors and Bioelectronics","volume":"271 ","pages":"117054"},"PeriodicalIF":10.7,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142823665","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A dual mode biosensor based on self-enhanced polyfluorene nanomaterial for fluorescence and electrochemiluminescence detection of Tau protein.","authors":"Jiaping Xie, Guomin Yang, Ruo Yuan, Shihong Chen","doi":"10.1016/j.bios.2024.117055","DOIUrl":"10.1016/j.bios.2024.117055","url":null,"abstract":"<p><p>Dual mode detection can overcome the poor anti-interference ability of single-mode detection, and greatly improve the detection accuracy. Fluorescence/electrochemiluminescence (FL/ECL) dual mode detection combines the advantages of FL and ECL, and has a promising application in bioanalysis. Common FL/ECL dual mode detection used different signal probes. Multiple signal probes inevitably have complex operation and uncontrollable differences between the two modes. In this work, poly [9,9-dioctylfluorenyl-2,7-diyl]-End-capped with 2,5-diphenyl-1,2,4-oxadiazole (PFO) was functionalized by sodium dodecyl sulfate (SDS) and polyethylenimine (PEI), and the resulting PFO-SDS-PEI nanoparticles (PSP NPs) exhibited excellent FL and ECL performance at the same time, so were creatively developed as mono-luminophore-based signal probe for FL/ECL dual-mode detection. The functional reagent SDS can significantly improve the water dispersion of luminophores, and PEI can not only provide amino functional groups for assembling biomolecules, but also significantly enhance the FL and ECL signals of the luminophores. Self-enhanced PSP NPs coupled strand displacement reaction (SDR) mediated by exonuclease Ⅲ (Exo Ⅲ) to achieve FL/ECL dual-mode detection of Tau protein, a biomarker of Alzheimer's disease (AD). The linear ranges for FL and ECL detection were 0.001-500 pg/mL and 0.00001-1.0 pg/mL and the limit of detections were 549.16 ag/mL and 5.45 ag/mL, respectively, showing good application potential. Self-enhanced PSP NPs provided an ideal mono-luminophore-based signal probe for FL/ECL dual-mode detection, and built a new method for detecting AD biomarker.</p>","PeriodicalId":259,"journal":{"name":"Biosensors and Bioelectronics","volume":"271 ","pages":"117055"},"PeriodicalIF":10.7,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142821547","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Electric field-induced alignment of Ag/Au nanowires for ultrasensitive in situ detection of Interleukin-6.","authors":"Huimin Li, Xinyu Li, Qi Meng, Junlei Han, Weilong Zhao, Jun Chen, Weiguang Su, Ming Song, Chaoyang Shi, Li Wang","doi":"10.1016/j.bios.2024.117033","DOIUrl":"10.1016/j.bios.2024.117033","url":null,"abstract":"<p><p>Interleukin-6 (IL-6) is a key parameter and critical role in cancer progression. However, for detection of IL-6 in colorectal cancer diagnosis, developing a sensitive biosensor is necessary and very important. In this paper, to enhance the sensitivity of IL-6 electrochemical biosensor, the electric field was used to orient arrangement of silver nanowires (AgNWs) to be free-standing AgNWs electrode. Gold nanoparticles (AuNPs) were electro-reduced around the surface of each AgNWs to solve the rapid oxidization problem of the AgNWs at very low potential (<70 mV) during the electrochemical detection. Oxidation peak current of the free-standing AgNWs/AuNPs electrode only decreased by 5% after 500 scanning cycles, while the free-standing AgNWs electrode without AuNPs decreased by 90.7% after 8 CV scanning cycles. The oxidation peak current of free-standing AgNWs/AuNPs electrode was 500 times of bare electrode. This biosensor showed a wide linear range from 0.001 ng ml^-1 to 100 ng ml^-1 and a low detection limit of 0.322 pg ml^-1 for IL-6. In the end, IL-6 secreted by Caco-2 cell was detected by the fabricated biosensor which integrated into the gut-on-a-chip. IL-6 secretion achieved 11.3 pg ml^-1 during 10-days culturing.</p>","PeriodicalId":259,"journal":{"name":"Biosensors and Bioelectronics","volume":"271 ","pages":"117033"},"PeriodicalIF":10.7,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142821549","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Advances in deep learning for personalized ECG diagnostics: A systematic review addressing inter-patient variability and generalization constraints.","authors":"Cheng Ding, Tianliang Yao, Chenwei Wu, Jianyuan Ni","doi":"10.1016/j.bios.2024.117073","DOIUrl":"10.1016/j.bios.2024.117073","url":null,"abstract":"<p><p>The Electrocardiogram (ECG) remains a fundamental tool in cardiac diagnostics, yet its interpretation has traditionally relied on cardiologists' expertise. Deep learning has revolutionized medical data analysis, especially within ECG diagnostics. However, the challenge of inter-patient variability limits the generalizability of ECG-AI models trained on population datasets, often reducing accuracy for specific patients or groups. While prior studies have developed various deep-learning techniques to address this issue, these advancements largely focus on universal models without tailoring to individual patient needs. A systematic review methodology was employed, comprehensively searching four major databases (PubMed, IEEE Xplore, Web of Science, and Google Scholar), meticulously screening and analyzing studies from 2020 to 2024 using a rigorous two-step selection process to ensure methodological quality and relevance, ultimately yielding 112 studies for comprehensive analysis. This review offers a unique perspective by systematically examining recent deep-learning approaches designed explicitly for personalized ECG diagnosis, emphasizing models that address patient-specific variability. Using a rigorous methodology for selecting and analyzing relevant studies, we provide an in-depth overview of advanced techniques, including transfer learning, generative adversarial networks, meta-learning, and domain adaptation. The review also investigates the limitations of these methods, such as balancing generalization with patient specificity and addressing data privacy concerns. By identifying these challenges and outlining future directions, this review highlights the transformative potential of deep learning for ECG diag-nostics in clinical practice. Our findings underscore a pathway toward more accurate, efficient, and patient-centered cardiac diagnostics, setting a foundation for future personalized care innovations.</p>","PeriodicalId":259,"journal":{"name":"Biosensors and Bioelectronics","volume":"271 ","pages":"117073"},"PeriodicalIF":10.7,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142870692","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Silk-polyurethane composite based flexible electrochemical biosensing platform for pathogen detection.","authors":"Karri Trinadha Rao, Rahul Gangwar, Aditya Bhagavathi, Sajmina Khatun, Pravat Kumar Sahu, Chandra Lekha Putta, Aravind Kumar Rengan, Challapalli Subrahmanyam, Suresh Kumar Garlapati, Siva Rama Krishna Vanjari","doi":"10.1016/j.bios.2024.117024","DOIUrl":"10.1016/j.bios.2024.117024","url":null,"abstract":"<p><p>The upcoming era of flexible and wearable electronics necessitates the development of low-cost, flexible, biocompatible substrates amenable to the fabrication of active devices such as electronic devices, sensors and transducers. While natural biopolymers such as Silk are robust and biocompatible, long-term flexibility is a concern due to the inherent brittle nature of soft Silk thin films. This work elucidates the preparation and characterization of Silk-polyurethane (Silk-PU) composite film that provides long-duration flexibility. More importantly, an electrochemical biosensing platform is developed by creating a three-electrode system using a screen-printing technique. The solvents in the Ink had little impact on the film. As a proof of concept, the detection of E. coli, a highly infectious pathogen, was demonstrated using screen-printed electrodes (SPEs) modified with gold nanoparticles. This method effectively detected E. coli across a wide range of concentrations, with a detection limit of 0.12 CFU/mL. The entire surface functionalization and detection process did not impact the Silk-PU substrate. Even after rigorous bending tests, the results were consistent, demonstrating the robustness and flexibility of the Silk-PU film. The platform demonstrated is scalable and amenable for multi-pathogen detection as it not only can integrate several working electrodes, each catering to detection of a particular pathogen, but also serve as a platform for lab-on-chip devices wherein PDMS-based microfluidics can be seamlessly integrated along with the proposed platform.</p>","PeriodicalId":259,"journal":{"name":"Biosensors and Bioelectronics","volume":"271 ","pages":"117024"},"PeriodicalIF":10.7,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142845439","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Injectable doxorubicin-loaded hyaluronic acid-based hydrogel for locoregional therapy and inhibiting metastasis of breast cancer.","authors":"Yongli Shi, Huiqing Zhu, Suyue Xu, Jingya Zhao, Yuxin Wang, Xiaofei Pan, Bingqian Zhao, Zeyu Sun, Yili Yin, Linyin Xu, Fengjiao Wei, Sisi He, Xueyan Hou, Jintao Xue","doi":"10.1016/j.colsurfb.2024.114433","DOIUrl":"10.1016/j.colsurfb.2024.114433","url":null,"abstract":"<p><p>Therapy and metastasis pose significant challenges for breast cancer therapy. Locoregional chemotherapy presents a promising strategy to address these dilemmas. In this study, a doxorubicin-loaded injectable hydrogel based on hyaluronic acid (DOX-MCHA^gel) was fabricated for locoregional chemotherapy and inhibiting the metastasis of breast cancer. The high bio-safety of cargo-free hydrogels (MCHA^gel) would enhance patient compliance. The sustained DOX release behaviors from DOX-MCHA^gel (over 10 days) could reduce dosing frequency and achieve long-term therapeutic effects. The potent in vivo anti-tumor activity of DOX-MCHA^gel was verified by the smallest tumor volumes, the largest number of apoptotic cells, and the strongest fluorescence intensity in TUNEL sections. Notably, the injectable DOX-MCHA^gel not only greatly suppressed the growth of 4T1 tumor tissues, but also effectively curbed the liver and lung metastasis in vivo. Moreover, the survival of 4T1-tumor bearing mice was extended without obvious systemic toxicity. In brief, the novel injectable hydrogel developed in this study offers a new strategy for locoregional therapy and inhibiting metastasis of breast cancer.</p>","PeriodicalId":279,"journal":{"name":"Colloids and Surfaces B: Biointerfaces","volume":"247 ","pages":"114433"},"PeriodicalIF":5.4,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142794138","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}