Nanomedicine: Nanotechnology, Biology and Medicine最新文献

Delivery of gene editing therapeutics 提供基因编辑疗法

IF 5.4 4区医学

Nanomedicine: Nanotechnology, Biology and Medicine Pub Date : 2023-11-01 DOI: 10.1016/j.nano.2023.102711

Bhavesh D. Kevadiya PhD , Farhana Islam B Pharm , Pallavi Deol PhD , Lubaba A. Zaman B Pharm , Dina A. Mosselhy PhD , Md Ashaduzzaman BS , Neha Bajwa PhD , Nanda Kishore Routhu PhD , Preet Amol Singh PhD , Shilpa Dawre PhD , Lalitkumar K. Vora PhD , Sumaiya Nahid M Pharm , Deepali Mathur PhD , Mohammad Ullah Nayan B Pharm , Ashish Baldi PhD , Ramesh Kothari PhD , Tapan A. Patel PhD , Jitender Madan PhD , Zahra Gounani PhD , Jitender Bariwal PhD , Howard E. Gendelman MD

{"title":"Delivery of gene editing therapeutics","authors":"Bhavesh D. Kevadiya PhD , Farhana Islam B Pharm , Pallavi Deol PhD , Lubaba A. Zaman B Pharm , Dina A. Mosselhy PhD , Md Ashaduzzaman BS , Neha Bajwa PhD , Nanda Kishore Routhu PhD , Preet Amol Singh PhD , Shilpa Dawre PhD , Lalitkumar K. Vora PhD , Sumaiya Nahid M Pharm , Deepali Mathur PhD , Mohammad Ullah Nayan B Pharm , Ashish Baldi PhD , Ramesh Kothari PhD , Tapan A. Patel PhD , Jitender Madan PhD , Zahra Gounani PhD , Jitender Bariwal PhD , Howard E. Gendelman MD","doi":"10.1016/j.nano.2023.102711","DOIUrl":"10.1016/j.nano.2023.102711","url":null,"abstract":"<div><p><span>For the past decades, gene editing demonstrated the potential to attenuate each of the root causes of genetic<span>, infectious, immune, cancerous, and degenerative disorders. More recently, Clustered Regularly Interspaced Short Palindromic Repeats-CRISPR-associated protein 9 (CRISPR-Cas9) editing proved effective for editing genomic, cancerous, or microbial DNA to limit disease onset or spread. However, the strategies to deliver CRISPR-Cas9 cargos and elicit protective immune responses requires safe delivery to disease targeted cells and tissues</span></span><em>.</em><span><span><span> While viral vector-based systems and viral particles demonstrate high efficiency and stable transgene expression, each are limited in their packaging capacities and secondary untoward immune responses. In contrast, the nonviral vector lipid nanoparticles were successfully used for as vaccine and therapeutic deliverables. Herein, we highlight each available </span>gene delivery systems for treating and preventing a broad range of infectious, inflammatory, genetic, and </span>degenerative diseases.</span></p></div><div><h3>Statement of significance</h3><p>CRISPR-Cas9 gene editing for disease treatment and prevention is an emerging field that can change the outcome of many chronic debilitating disorders.</p></div>","PeriodicalId":396,"journal":{"name":"Nanomedicine: Nanotechnology, Biology and Medicine","volume":"54 ","pages":"Article 102711"},"PeriodicalIF":5.4,"publicationDate":"2023-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41183123","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Coating influence on inner shell water exchange: An underinvestigated major contributor to SPIONs relaxation properties 涂层对内壳水交换的影响:一个未被充分研究的SPIONs弛豫特性的主要贡献者

IF 5.4 4区医学

Nanomedicine: Nanotechnology, Biology and Medicine Pub Date : 2023-11-01 DOI: 10.1016/j.nano.2023.102713

Yusong Peng , Yunlong Li , Li Li , Manman Xie , Yiqing Wang , Christopher J. Butch

{"title":"Coating influence on inner shell water exchange: An underinvestigated major contributor to SPIONs relaxation properties","authors":"Yusong Peng , Yunlong Li , Li Li , Manman Xie , Yiqing Wang , Christopher J. Butch","doi":"10.1016/j.nano.2023.102713","DOIUrl":"10.1016/j.nano.2023.102713","url":null,"abstract":"<div><p><span>Superparamagnetic iron oxide<span> nanoparticles<span><span><span> (SPIONs) are heavily studied as potential MRI contrast enhancing agents. Every year, novel coatings are reported which yield large increases in relaxivity compared to similar particles. However, the reason for the increased performance is not always well understood mechanistically. In this review, we attempt to relate these advances back to fundamental models of relaxivity, developed for chelated </span>metal ions, primarily </span>gadolinium. We focus most closely on the three-shell model which considers the relaxation of surface-bound, entrained, and bulk water molecules as three distinct contributions to total relaxation. Because SPIONs are larger, more complex, and entrain significantly more water than gadolinium-based contrast agents, we consider how to adapt the application of classical models to SPIONs in a predictive manner. By carefully considering models and previous results, a qualitative model of </span></span></span>entrained water<span> interactions emerges, based primarily on the contributions of core size, coating thickness, density, and hydrophilicity.</span></p></div>","PeriodicalId":396,"journal":{"name":"Nanomedicine: Nanotechnology, Biology and Medicine","volume":"54 ","pages":"Article 102713"},"PeriodicalIF":5.4,"publicationDate":"2023-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41237043","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Liposomes - Human phagocytes interplay in whole blood: effect of liposome design 脂质体-人类吞噬细胞在全血中的相互作用：脂质体设计的影响。

IF 5.4 4区医学

Nanomedicine: Nanotechnology, Biology and Medicine Pub Date : 2023-11-01 DOI: 10.1016/j.nano.2023.102712

Miriam Giambelluca PhD , Elena Markova MPharm , Claire Louet PhD , Bjørg Steinkjer MSc , Rune Sundset PhD , Nataša Škalko-Basnet PhD , Sjoerd Hak PhD

{"title":"Liposomes - Human phagocytes interplay in whole blood: effect of liposome design","authors":"Miriam Giambelluca PhD , Elena Markova MPharm , Claire Louet PhD , Bjørg Steinkjer MSc , Rune Sundset PhD , Nataša Škalko-Basnet PhD , Sjoerd Hak PhD","doi":"10.1016/j.nano.2023.102712","DOIUrl":"10.1016/j.nano.2023.102712","url":null,"abstract":"<div><p>Nanomedicine holds immense potential for therapeutic manipulation of phagocytic immune cells. However, <em>in vitro</em> studies often fail to accurately translate to the complex <em>in vivo</em> environment. To address this gap, we employed an <em>ex vivo</em> human whole-blood assay to evaluate liposome interactions with immune cells. We systematically varied liposome size, PEG-surface densities and sphingomyelin and ganglioside content. We observed differential uptake patterns of the assessed liposomes by neutrophils and monocytes, emphasizing the importance of liposome design. Interestingly, our results aligned closely with published <em>in vivo</em> observations in mice and patients. Moreover, liposome exposure induced changes in cytokine release and cellular responses, highlighting the potential modulation of immune system. Our study highlights the utility of human whole-blood models in assessing nanoparticle-immune cell interactions and provides insights into liposome design for modulating immune responses.</p></div>","PeriodicalId":396,"journal":{"name":"Nanomedicine: Nanotechnology, Biology and Medicine","volume":"54 ","pages":"Article 102712"},"PeriodicalIF":5.4,"publicationDate":"2023-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S1549963423000631/pdfft?md5=5125fbbe8319c4a5937eb9ef1f71ad02&pid=1-s2.0-S1549963423000631-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41206517","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Fabrication of blended nanofibrous cardiac patch transplanted with TGF-β3 and human umbilical cord MSCs-derived exosomes for potential cardiac regeneration after acute myocardial infarction 用TGF-β3和人脐带MSCs衍生的外泌体移植的混合纳米纤维心脏贴片的制备，用于急性心肌梗死后潜在的心脏再生。

IF 5.4 4区医学

Nanomedicine: Nanotechnology, Biology and Medicine Pub Date : 2023-11-01 DOI: 10.1016/j.nano.2023.102708

Ping Ping MD , Shasha Guan MD , Chaoxue Ning MD , Ting Yang MD , Yali Zhao MD , Pei Zhang MD , Zhitao Gao MD , Shihui Fu MD

{"title":"Fabrication of blended nanofibrous cardiac patch transplanted with TGF-β3 and human umbilical cord MSCs-derived exosomes for potential cardiac regeneration after acute myocardial infarction","authors":"Ping Ping MD , Shasha Guan MD , Chaoxue Ning MD , Ting Yang MD , Yali Zhao MD , Pei Zhang MD , Zhitao Gao MD , Shihui Fu MD","doi":"10.1016/j.nano.2023.102708","DOIUrl":"10.1016/j.nano.2023.102708","url":null,"abstract":"<div><p><span><span>Acute myocardial infarction (AMI) is a common </span>cardiovascular condition<span><span> that progressively results in heart failure. In the present study, we have designed to load transforming growth factor beta<span><span> 3 (TGF-β3) and cardio potential exosomes<span> into the blended polycaprolactone/type I collagen (PCL/COL-1) nanofibrous patch (Exo@TGF-β3@NFs) and examined its feasibility for cardiac repair. The </span></span>bioactivity of the developed NFs towards the migration and proliferation of </span></span>human umbilical vein endothelial cells was determined using </span></span><em>in vitro</em><span> cell compatibility assays. Additionally, Exo@TGF-β3/NFs showed up-regulation of genes involved in angiogenesis<span> and mesenchymal differentiations </span></span><em>in vitro</em>. The <em>in vivo</em><span><span> experiments performed 4 weeks after transplantation showed that the Exo@TGF-β3@NFs had a higher LV ejection fraction<span> and fraction shortening functions. Subsequently, it has been determined that Exo@TGF-β3@NFs significantly reduced AMI size and fibrosis and increased scar thickness. The developed NFs approach will become a useful therapeutic approach for the </span></span>treatment of AMI.</span></p></div>","PeriodicalId":396,"journal":{"name":"Nanomedicine: Nanotechnology, Biology and Medicine","volume":"54 ","pages":"Article 102708"},"PeriodicalIF":5.4,"publicationDate":"2023-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41134516","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

State-of-the-art and future perspectives in infertility diagnosis: Conventional versus nanotechnology-based assays 不孕不育诊断的现状和未来前景：传统与基于纳米技术的检测。

IF 5.4 4区医学

Nanomedicine: Nanotechnology, Biology and Medicine Pub Date : 2023-11-01 DOI: 10.1016/j.nano.2023.102709

Bianca-Astrid Andone MSc , Iuliana M. Handrea-Dragan MSc , Ioan Botiz PhD , Sanda Boca

{"title":"State-of-the-art and future perspectives in infertility diagnosis: Conventional versus nanotechnology-based assays","authors":"Bianca-Astrid Andone MSc , Iuliana M. Handrea-Dragan MSc , Ioan Botiz PhD , Sanda Boca","doi":"10.1016/j.nano.2023.102709","DOIUrl":"10.1016/j.nano.2023.102709","url":null,"abstract":"<div><p><span><span><span>According to the latest World Health Organization statistics, around 50 to 80 million people worldwide suffer from infertility, amongst which male factors are responsible for around 20 to 30 % of all infertility cases while 50 % were attributed to the female ones. As it is becoming a recurrent </span>health problem<span> worldwide, clinicians require more accurate methods for the improvement of both diagnosis and treatment schemes. By emphasizing the potential use of innovative methods for the rapid identification of the infertility causes, this review presents the news from this dynamic domain and highlights the benefits brought by emerging research fields. A systematic description of the standard techniques used in clinical protocols for diagnosing infertility in both genders is firstly provided, followed by the presentation of more accurate and comprehensive nanotechnology-related analysis methods such as nanoscopic-resolution imaging, biosensing approaches and assays that employ </span></span>nanomaterials<span> in their design. Consequently, the implementation of nanotechnology related tools in clinical practice, as recently demonstrated in the selection of spermatozoa, the detection of key proteins in the </span></span>fertilization<span> process or the testing of DNA integrity or the evaluation of oocyte quality, might confer excellent advantages both for improving the assessment of infertility, and for the success of the fertilization process.</span></p></div>","PeriodicalId":396,"journal":{"name":"Nanomedicine: Nanotechnology, Biology and Medicine","volume":"54 ","pages":"Article 102709"},"PeriodicalIF":5.4,"publicationDate":"2023-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10634107","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Insights on the molecular mechanisms of cytotoxicity induced by AS1411 linked to folate-functionalized DNA nanocages in cancer cells 癌细胞中与叶酸功能化DNA纳米笼相关的AS1411诱导细胞毒性的分子机制

IF 5.5 4区医学

Nanomedicine: Nanotechnology, Biology and Medicine Pub Date : 2023-09-19 DOI: 10.1016/j.nano.2023.102710

Valeria Unida PhD , Eleonora Mangano PhD , Tania Camboni PhD , Clarissa Consolandi PhD , Alessandro Desideri PhD , Marco Severgnini MSc , Ingrid Cifola PhD , Silvia Biocca PhD

{"title":"Insights on the molecular mechanisms of cytotoxicity induced by AS1411 linked to folate-functionalized DNA nanocages in cancer cells","authors":"Valeria Unida PhD , Eleonora Mangano PhD , Tania Camboni PhD , Clarissa Consolandi PhD , Alessandro Desideri PhD , Marco Severgnini MSc , Ingrid Cifola PhD , Silvia Biocca PhD","doi":"10.1016/j.nano.2023.102710","DOIUrl":"https://doi.org/10.1016/j.nano.2023.102710","url":null,"abstract":"<div><p><span><span>Self-assembled multivalent DNA </span>nanocages<span><span> are an emerging class of molecules useful for biomedicine<span> applications. Here, we investigated the molecular mechanisms of cytotoxicity induced by AS1411 free aptamer, AS1411-linked nanocages (Apt-NCs) and nanocages harboring both </span></span>folate and AS1411 </span></span>functionalization<span><span> (Fol-Apt-NCs) in HeLa and MDA-MB-231 cancer cell lines. The three </span>treatments<span><span> showed different cytotoxic efficacy and Fol-Apt-NCs resulted the most effective in inhibiting cell proliferation and inducing apoptotic pathways and </span>ROS<span><span> activation in both HeLa and MDA-MB-231 cells. RNA-seq analysis allowed to identify biological functions and genes altered by the various treatments, depending on the AS1411 route of intracellular entry, highlighting the different behavior of the two cancer cell lines. Notably, Fol-Apt-NCs altered the expression of a subset of genes associated to cancer chemoresistance in MDA-MB-231, but not in HeLa cells, and this may explain the increased </span>chemosensitivity<span> to drugs<span> delivered through DNA nanocages of the triple-negative breast cancer cells.</span></span></span></span></span></p></div>","PeriodicalId":396,"journal":{"name":"Nanomedicine: Nanotechnology, Biology and Medicine","volume":"54 ","pages":"Article 102710"},"PeriodicalIF":5.5,"publicationDate":"2023-09-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"39965202","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Efficacy comparisons of solvent-based paclitaxel, liposomal paclitaxel, nanoparticle albumin-bound paclitaxel, and docetaxel after neoadjuvant systemic treatment in breast cancer 溶剂型紫杉醇、脂质体紫杉醇、纳米颗粒白蛋白结合紫杉醇和多西紫杉醇在乳腺癌新辅助全身治疗后的疗效比较

IF 5.5 4区医学

Nanomedicine: Nanotechnology, Biology and Medicine Pub Date : 2023-09-17 DOI: 10.1016/j.nano.2023.102707

Weiwei Zhang MSc , Ye Wang BMed , Jinzhi He BMed , Yinggang Xu MSc , Rui Chen MSc , Xinyu Wan BMed , Wenjie Shi BMed , Xiaofeng Huang MSc , Lu Xu MD , Jue Wang MD , Xiaoming Zha MD

{"title":"Efficacy comparisons of solvent-based paclitaxel, liposomal paclitaxel, nanoparticle albumin-bound paclitaxel, and docetaxel after neoadjuvant systemic treatment in breast cancer","authors":"Weiwei Zhang MSc , Ye Wang BMed , Jinzhi He BMed , Yinggang Xu MSc , Rui Chen MSc , Xinyu Wan BMed , Wenjie Shi BMed , Xiaofeng Huang MSc , Lu Xu MD , Jue Wang MD , Xiaoming Zha MD","doi":"10.1016/j.nano.2023.102707","DOIUrl":"https://doi.org/10.1016/j.nano.2023.102707","url":null,"abstract":"<div><h3>Purpose</h3><p>There are four kinds of taxanes<span>: solvent-based paclitaxel<span><span> (Sb-P), liposomal paclitaxel (Lps-P), nanoparticle albumin-bound paclitaxel (Nab-P), and docetaxel. This study aims to retrospectively evaluate the efficacy of different taxanes on neoadjuvant </span>systemic treatment (NST) in breast cancer.</span></span></p></div><div><h3>Methods</h3><p>Patients who were diagnosed with breast cancer and had received integral NST from August 2013 to April 2022 were enrolled. The efficacy was divided into total pathological complete response (total-pCR), breast pathological complete response (breast-pCR), and axillary pathological complete response (axillary-pCR) for in-depth analysis and discussion.</p></div><div><h3>Results</h3><p>The choice of taxane was an independent risk factor for total-pCR and breast-pCR rates. The highest total-pCR and breast-pCR rates were found in the Nab-P group. The difference was not significant among all the taxanes in the axillary-pCR rate.</p></div><div><h3>Conclusion</h3><p>Nab-P significantly improved the total-pCR and breast-pCR rates. It should be the first choice among taxanes in NST for breast cancer.</p></div>","PeriodicalId":396,"journal":{"name":"Nanomedicine: Nanotechnology, Biology and Medicine","volume":"54 ","pages":"Article 102707"},"PeriodicalIF":5.5,"publicationDate":"2023-09-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"39965220","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Microfluidic-derived docosahexaenoic acid liposomes for glioblastoma therapy 微流体衍生的二十二碳六烯酸脂质体用于胶质母细胞瘤治疗

IF 5.5 4区医学

Nanomedicine: Nanotechnology, Biology and Medicine Pub Date : 2023-09-01 DOI: 10.1016/j.nano.2023.102704

D. Mendanha MSc , S. Gimondi PhD , B.M. Costa PhD , H. Ferreira PhD , N.M. Neves PhD

{"title":"Microfluidic-derived docosahexaenoic acid liposomes for glioblastoma therapy","authors":"D. Mendanha MSc , S. Gimondi PhD , B.M. Costa PhD , H. Ferreira PhD , N.M. Neves PhD","doi":"10.1016/j.nano.2023.102704","DOIUrl":"https://doi.org/10.1016/j.nano.2023.102704","url":null,"abstract":"<div><p>Glioblastoma (GBM) is the most prevalent malignant primary brain tumor and currently lacks an effective treatment. In this study, we utilized a microfluidic system to synthesize docosahexaenoic acid (DHA) liposomes for GBM therapy. DHA is an omega-3 (ω3) polyunsaturated fatty acid commonly found in human dietary consumption that has demonstrated potential in mitigating cancer development. The microfluidic device employed allowed for precise fine-tuning of the physicochemical properties of liposomes by adjusting the flow rate ratios, flow rates, and lipid concentrations. Three distinct-sized liposomes, ranging from 80 nm and 130 nm, were successfully internalized by GBM cells, and demonstrated the ability to reduce the viability of these cells. Furthermore, DHA liposomes proved significantly more efficient in triggering apoptotic pathways, through caspase-3-dependent mechanisms, in comparison to free DHA. Thus, the nanomedicine platform established in this study presents new opportunities in the development of liposome formulations incorporating ω3 fatty acids for cancer therapy.</p></div>","PeriodicalId":396,"journal":{"name":"Nanomedicine: Nanotechnology, Biology and Medicine","volume":"53 ","pages":"Article 102704"},"PeriodicalIF":5.5,"publicationDate":"2023-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"2250158","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Rolipram-loaded PgP nanoparticle reduces secondary injury and enhances motor function recovery in a rat moderate contusion SCI model 罗利普兰负载PgP纳米颗粒减少中度挫伤大鼠模型的继发性损伤并增强运动功能恢复

IF 5.5 4区医学

Nanomedicine: Nanotechnology, Biology and Medicine Pub Date : 2023-09-01 DOI: 10.1016/j.nano.2023.102702

Jun Gao PhD , Min Kyung Khang PhD , Zhen Liao BS , Ken Webb PhD , Megan Ryan Detloff PhD , Jeoung Soo Lee PhD

{"title":"Rolipram-loaded PgP nanoparticle reduces secondary injury and enhances motor function recovery in a rat moderate contusion SCI model","authors":"Jun Gao PhD , Min Kyung Khang PhD , Zhen Liao BS , Ken Webb PhD , Megan Ryan Detloff PhD , Jeoung Soo Lee PhD","doi":"10.1016/j.nano.2023.102702","DOIUrl":"https://doi.org/10.1016/j.nano.2023.102702","url":null,"abstract":"<div><p>Spinal cord injury<span> (SCI) results in immediate axonal damage and cell death<span><span>, as well as a prolonged secondary injury consist of a cascade of pathophysiological processes. One important aspect of secondary injury is activation of phosphodiesterase<span> 4 (PDE4) that leads to reduce cAMP levels in the injured spinal cord. We have developed an amphiphilic copolymer, poly (lactide-co-glycolide)-graft-polyethylenimine (PgP) that can deliver </span></span>Rolipram<span>, the PDE4 inhibitor. The objective of this work was to investigate the effect of rolipram loaded PgP (Rm-PgP) on secondary injury and motor functional recovery in a rat moderate contusion SCI model. We observed that Rm-PgP can increase cAMP level at the lesion site, and reduce secondary injury such as the inflammatory response by macrophages/microglia, astrogliosis by activated astrocytes and apoptosis as well as improve neuronal survival at 4 weeks post-injury (WPI). We also observed that Rm-PgP can improve motor functional recovery after SCI over 4 WPI.</span></span></span></p></div>","PeriodicalId":396,"journal":{"name":"Nanomedicine: Nanotechnology, Biology and Medicine","volume":"53 ","pages":"Article 102702"},"PeriodicalIF":5.5,"publicationDate":"2023-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"3463182","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Novel pH-responsive E-selectin targeting natural polysaccharides hybrid micelles for diabetic nephropathy 针对糖尿病肾病的天然多糖杂交胶束的新型ph反应性e -选择素

IF 5.5 4区医学

Nanomedicine: Nanotechnology, Biology and Medicine Pub Date : 2023-08-01 DOI: 10.1016/j.nano.2023.102696

Chunjing Guo Ph.D , Min Cao MD , Ningning Diao MD , Wenxin Wang MD , Hongxu Geng MD , Yanguo Su MD , Tianying Sun BD , Xinyue Lu BD , Ming Kong Ph.D , Daquan Chen Ph.D

{"title":"Novel pH-responsive E-selectin targeting natural polysaccharides hybrid micelles for diabetic nephropathy","authors":"Chunjing Guo Ph.D , Min Cao MD , Ningning Diao MD , Wenxin Wang MD , Hongxu Geng MD , Yanguo Su MD , Tianying Sun BD , Xinyue Lu BD , Ming Kong Ph.D , Daquan Chen Ph.D","doi":"10.1016/j.nano.2023.102696","DOIUrl":"https://doi.org/10.1016/j.nano.2023.102696","url":null,"abstract":"<div><p><span>Diabetic nephropathy<span><span> (DN) is an important complication of diabetes and is the main cause of end-stage renal disease. The pathogenesis of DN is complex, including glucose and </span>lipid metabolism disorder<span><span><span><span>, inflammation, and so on. Novel hybrid micelles loaded </span>Puerarin<span><span> (Pue) based on Angelica sinensis </span>polysaccharides (ASP) and </span></span>Astragalus polysaccharide (APS) were fabricated with pH-responsive ASP-hydrazone-ibuprofen (BF) materials (ASP-HZ-BF, SHB) and </span>sialic acid (SA) modified APS-hydrazone-ibuprofen materials (SA/APS-HZ-BF, SPHB) by thin-film dispersion method. The SA in hybrid micelles can specifically bind to the </span></span></span><em>E</em><span><span>-selectin receptor which is highly expressed in inflammatory vascular endothelial cells<span>. The loaded Pue could be accurately delivered to the inflammatory site of the kidney in response to the low pH microenvironment. Overall, this study provides a promising strategy for developing hybrid micelles based on natural polysaccharides for the </span></span>treatment of diabetic nephropathy by inhibiting renal inflammatory reactions, and antioxidant stress.</span></p></div>","PeriodicalId":396,"journal":{"name":"Nanomedicine: Nanotechnology, Biology and Medicine","volume":"52 ","pages":"Article 102696"},"PeriodicalIF":5.5,"publicationDate":"2023-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"2359721","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1