Nanomedicine: Nanotechnology, Biology and Medicine最新文献_第2页

Multi-stimuli-responsive chitosan-functionalized magnetite/poly(ε-caprolactone) nanoparticles as theranostic platforms for combined tumor magnetic resonance imaging and chemotherapy 多刺激响应壳聚糖功能化磁铁矿/聚(ε-己内酯)纳米颗粒作为肿瘤磁共振成像和化疗联合治疗平台

IF 5.5 4区医学

Nanomedicine: Nanotechnology, Biology and Medicine Pub Date : 2023-08-01 DOI: 10.1016/j.nano.2023.102695

Gracia García-García PhD , Carlos Caro PhD , Fátima Fernández-Álvarez PhD , María Luisa García-Martín PhD , José L. Arias PhD

{"title":"Multi-stimuli-responsive chitosan-functionalized magnetite/poly(ε-caprolactone) nanoparticles as theranostic platforms for combined tumor magnetic resonance imaging and chemotherapy","authors":"Gracia García-García PhD , Carlos Caro PhD , Fátima Fernández-Álvarez PhD , María Luisa García-Martín PhD , José L. Arias PhD","doi":"10.1016/j.nano.2023.102695","DOIUrl":"https://doi.org/10.1016/j.nano.2023.102695","url":null,"abstract":"<div>Chitosan-functionalized magnetite/poly(ε-caprolactone) nanoparticles were formulated by interfacial polymer disposition plus coacervation, and loaded with gemcitabine. That (core/shell)/shell nanostructure was confirmed by electron microscopy, elemental analysis, electrophoretic, and Fourier transform infrared characterizations. A short-term stability study proved the protection against particle aggregation provided by the chitosan shell. Superparamagnetic properties of the nanoparticles were characterized in vitro, while the definition of the longitudinal and transverse relaxivities was an initial indication of their capacity as T2 contrast agents. Safety of the particles was demonstrated in vitro on HFF-1 human fibroblasts, and ex vivo on SCID mice. The nanoparticles demonstrated in vitro pH- and heat-responsive gemcitabine release capabilities. In vivo magnetic resonance imaging studies and Prussian blue visualization of iron deposits in tissue samples defined the improvement in nanoparticle targeting into the tumor when using a magnetic field. This tri-stimuli (magnetite/poly(ε-caprolactone))/chitosan nanostructure could find theranostic applications (biomedical imaging & chemotherapy) against tumors.</div>","PeriodicalId":396,"journal":{"name":"Nanomedicine: Nanotechnology, Biology and Medicine","volume":"52 ","pages":"Article 102695"},"PeriodicalIF":5.5,"publicationDate":"2023-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"3341936","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Functional attachment of primary neurons and glia on radiopaque implantable biomaterials for nerve repair 初级神经元和胶质细胞在不透射线植入式神经修复材料上的功能附着

IF 5.5 4区医学

Nanomedicine: Nanotechnology, Biology and Medicine Pub Date : 2023-08-01 DOI: 10.1016/j.nano.2023.102692

Kendell M. Pawelec PhD , Jeremy M.L. Hix LATG , Erik M. Shapiro PhD

{"title":"Functional attachment of primary neurons and glia on radiopaque implantable biomaterials for nerve repair","authors":"Kendell M. Pawelec PhD , Jeremy M.L. Hix LATG , Erik M. Shapiro PhD","doi":"10.1016/j.nano.2023.102692","DOIUrl":"https://doi.org/10.1016/j.nano.2023.102692","url":null,"abstract":"<div>Repairing peripheral nerve injuries remains a challenge, even with use of auxiliary implantable biomaterial conduits. After implantation the location or function of polymeric devices cannot be assessed via clinical imaging modalities. Adding nanoparticle contrast agents into polymers can introduce radiopacity enabling imaging using computed tomography. Radiopacity must be balanced with changes in material properties impacting device function. In this study radiopaque composites were made from polycaprolactone and poly(lactide-co-glycolide) 50:50 and 85:15 with 0–40 wt% tantalum oxide (TaOx) nanoparticles. To achieve radiopacity, ≥5 wt% TaOx was required, with ≥20 wt% TaOx reducing mechanical properties and causing nanoscale surface roughness. Composite films facilitated nerve regeneration in an in vitro co-culture of adult glia and neurons, measured by markers for myelination. The ability of radiopaque films to support regeneration was driven by the properties of the polymer, with 5–20 wt% TaOx balancing imaging functionality with biological response and proving that in situ monitoring is feasible.</div>","PeriodicalId":396,"journal":{"name":"Nanomedicine: Nanotechnology, Biology and Medicine","volume":"52 ","pages":"Article 102692"},"PeriodicalIF":5.5,"publicationDate":"2023-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"2359030","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Analysis of Adeno-Associated Virus Serotype 8 (AAV8)-antibody complexes using epitope mapping by molecular imprinting leads to the identification of Fab peptides that potentially evade AAV8 neutralisation 利用分子印迹技术对腺相关病毒血清型8 (AAV8)抗体复合物进行表位定位分析，从而鉴定出可能逃避AAV8中和的Fab肽

IF 5.5 4区医学

Nanomedicine: Nanotechnology, Biology and Medicine Pub Date : 2023-08-01 DOI: 10.1016/j.nano.2023.102691

Elena Piletska PhD , Philippe Veron PhD , Bérangère Bertin PhD , Federico Mingozzi , Donald Jones Professor, PhD , Rachel L. Norman PhD , Joseph Earley MSc , Kal Karim PhD , Alvaro Garcia-Cruz PhD , Sergey Piletsky Professor, PhD

{"title":"Analysis of Adeno-Associated Virus Serotype 8 (AAV8)-antibody complexes using epitope mapping by molecular imprinting leads to the identification of Fab peptides that potentially evade AAV8 neutralisation","authors":"Elena Piletska PhD , Philippe Veron PhD , Bérangère Bertin PhD , Federico Mingozzi , Donald Jones Professor, PhD , Rachel L. Norman PhD , Joseph Earley MSc , Kal Karim PhD , Alvaro Garcia-Cruz PhD , Sergey Piletsky Professor, PhD","doi":"10.1016/j.nano.2023.102691","DOIUrl":"https://doi.org/10.1016/j.nano.2023.102691","url":null,"abstract":"<div>Gene therapy is a promising approach for treating genetic disorders by delivering therapeutic genes to replace or correct malfunctioning genes. However, the introduced gene therapy vector can trigger an immune response, leading to reduced efficacy and potential harm to the patient. To improve the efficiency and safety of gene therapy, preventing the immune response to the vector is crucial. This can be achieved through the use of immunosuppressive drugs, vector engineering to evade the immune system, or delivery methods that bypass the immune system altogether. By reducing the immune response, gene therapy can deliver therapeutic genes more effectively and potentially cure genetic diseases. In this study, a novel molecular imprinting technique, combined with mass-spectrometry and bioinformatics, was used to identify four antigen-binding fragments (Fab) sequences of Adeno-Associated Virus (AAV) - neutralising antibodies capable of binding to AAV. The identified Fab peptides were shown to prevent AAV8's binding to antibodies, demonstrating their potential to improve gene therapy efficiency by preventing the immune response.</div>","PeriodicalId":396,"journal":{"name":"Nanomedicine: Nanotechnology, Biology and Medicine","volume":"52 ","pages":"Article 102691"},"PeriodicalIF":5.5,"publicationDate":"2023-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"1567230","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Encapsulation into hyaluronic acid-based nanogels improves the selectivity index of the snake cathelicidin Ab-Cath 透明质酸纳米凝胶包封提高了蛇抗菌素Ab-Cath的选择性指数

IF 5.5 4区医学

Nanomedicine: Nanotechnology, Biology and Medicine Pub Date : 2023-08-01 DOI: 10.1016/j.nano.2023.102694

Miriam E. van Gent PhD , Sylvia N. Kłodzińska PhD , Maureen Severin BSc , Muhanad Ali PhD , Bjorn R. van Doodewaerd BSc , Erik Bos MSc , Roman I. Koning Assistant professor , Jan Wouter Drijfhout Associate professor , Hanne M. Nielsen Professor , Peter H. Nibbering Associate professor

{"title":"Encapsulation into hyaluronic acid-based nanogels improves the selectivity index of the snake cathelicidin Ab-Cath","authors":"Miriam E. van Gent PhD , Sylvia N. Kłodzińska PhD , Maureen Severin BSc , Muhanad Ali PhD , Bjorn R. van Doodewaerd BSc , Erik Bos MSc , Roman I. Koning Assistant professor , Jan Wouter Drijfhout Associate professor , Hanne M. Nielsen Professor , Peter H. Nibbering Associate professor","doi":"10.1016/j.nano.2023.102694","DOIUrl":"https://doi.org/10.1016/j.nano.2023.102694","url":null,"abstract":"<div>The antimicrobial peptide Ab-Cath, is a promising candidate for development as treatment for antimicrobial resistant (AMR) bacterial infections. Future clinical use is hampered by Ab-Cath's cationic peptidic nature and limited therapeutic window. Here, we evaluated hyaluronic acid-based nanogels for encapsulation of Ab-Cath to circumvent these limitations. Using microfluidics, monodispersed anionic nanogels of 156–232 nm encapsulating >99 % Ab-Cath were prepared. Unprecedented, lyophilization using polyvinyl alcohol and dextran-40 provided Ab-Cath nanogel protection and allowed easy dose adjustment. Lyophilized and redispersed Ab-Cath nanogels were as effective as Ab-Cath solution in killing AMR Staphylococcus aureus, Acinetobacter baumannii and Escherichia coli in biological fluids, and in reducing S. aureus and A. baumannii biofilms. Importantly, encapsulation of Ab-Cath in nanogels reduced Ab-Cath's cytotoxic effects on human fibroblasts by ≥10-fold. Moreover, cutaneous application of Ab-Cath nanogels eliminated bacteria colonizing 3D human skin. These findings affirm the use of nanogels to increase the selectivity index of antimicrobial peptides.</div>","PeriodicalId":396,"journal":{"name":"Nanomedicine: Nanotechnology, Biology and Medicine","volume":"52 ","pages":"Article 102694"},"PeriodicalIF":5.5,"publicationDate":"2023-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"3341937","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Intranasal in situ gelling liquid crystal for delivery of resveratrol ameliorates memory and neuroinflammation in Alzheimer's disease 鼻内原位胶凝液晶传递白藜芦醇改善阿尔茨海默病的记忆和神经炎症

IF 5.5 4区医学

Nanomedicine: Nanotechnology, Biology and Medicine Pub Date : 2023-07-01 DOI: 10.1016/j.nano.2023.102689

Bruno Fonseca-Santos PhD , Camila André Cazarin MSc , Patrícia Bento da Silva PhD , Kaio Pini dos Santos PhD , Márcia Cristina Oliveira da Rocha PhD , Sônia Nair Báo PhD , Márcia Maria De-Souza PhD , Marlus Chorilli PhD

{"title":"Intranasal in situ gelling liquid crystal for delivery of resveratrol ameliorates memory and neuroinflammation in Alzheimer's disease","authors":"Bruno Fonseca-Santos PhD , Camila André Cazarin MSc , Patrícia Bento da Silva PhD , Kaio Pini dos Santos PhD , Márcia Cristina Oliveira da Rocha PhD , Sônia Nair Báo PhD , Márcia Maria De-Souza PhD , Marlus Chorilli PhD","doi":"10.1016/j.nano.2023.102689","DOIUrl":"https://doi.org/10.1016/j.nano.2023.102689","url":null,"abstract":"<div>Alzheimer's disease (AD) is an illness that affects people aged 65 or older and affects around 6.5 million in the United States. Resveratrol is a chemical obtained from natural products and it exhibits biological activity based on inhibiting the formation, depolymerization of the amyloid, and decreasing neuroinflammation. Due to the insolubility of this compound; its incorporation in surfactant-based systems was proposed to design an intranasal formulation. A range of systems has been produced by mixing oleic acid, CETETH-20 and water. Polarised light microscopy (PLM), small angle x-ray scattering (SAXS) and transmission electron microscopy (TEM) confirm the initial liquid formulation (F) presented as microemulsion (ME). After dilution, the gelled systems were characterized as hexagonal mesophase and they showed feasibility proprieties. Pharmacological assays performed after intranasal administration showed the ability to improve learning and memory in animals, as well as remission of neuroinflammation via inhibition of interleukin.</div>","PeriodicalId":396,"journal":{"name":"Nanomedicine: Nanotechnology, Biology and Medicine","volume":"51 ","pages":"Article 102689"},"PeriodicalIF":5.5,"publicationDate":"2023-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"3206325","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 2

Directional conjugation of Trop2 antibody to black phosphorus nanosheets for phototherapy in orthotopic gastric carcinoma Trop2抗体与黑磷纳米片定向偶联用于原位胃癌光疗

IF 5.5 4区医学

Nanomedicine: Nanotechnology, Biology and Medicine Pub Date : 2023-07-01 DOI: 10.1016/j.nano.2023.102687

Lizhou Jia PhD , Yuhao Fu MSc , Ning Zhang MB , Yang Liu MB , Lin Su MSc , Haisheng Wang PhD , Wei Zhao PhD

{"title":"Directional conjugation of Trop2 antibody to black phosphorus nanosheets for phototherapy in orthotopic gastric carcinoma","authors":"Lizhou Jia PhD , Yuhao Fu MSc , Ning Zhang MB , Yang Liu MB , Lin Su MSc , Haisheng Wang PhD , Wei Zhao PhD","doi":"10.1016/j.nano.2023.102687","DOIUrl":"https://doi.org/10.1016/j.nano.2023.102687","url":null,"abstract":"<div>Tumor-associated calcium signal transducer 2 (Trop2) is highly specific expressed in gastric carcinoma (GC). The combination of Trop2 antibody and phototherapy agents could exhibit synergetic antitumor activity. Black phosphorus nanosheets (BP) are covalently modified with Trop2 IgG antibodies via heterobifunctional linker of polyethylene glycol (PEG). Then the Trop2 antibody was directionally conjugated to BP via Schiff base reaction between aldehyde group from oxidized Trop2 antibody and amino group of PEG. The Trop2-functionalzied BP can significantly increase the endocytosis of BP in Trop2-positive GC cells exhibiting a reinforced antitumor activity under near infrared (NIR) irradiation. More importantly, a murine orthotopic GC model demonstrates that Trop2 antibody modification can significantly promote the accumulation of BP at tumor tissues and strengthen antitumoral activity of phototherapy. Directional conjugation of Trop2 antibody to BP facilitates the BP with superior stability, tumor targeting ability and excellent anti-tumor activity under NIR irradiation without systemic toxicity.</div>","PeriodicalId":396,"journal":{"name":"Nanomedicine: Nanotechnology, Biology and Medicine","volume":"51 ","pages":"Article 102687"},"PeriodicalIF":5.5,"publicationDate":"2023-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"3456752","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Autocatalytically hydroxyl-producing composite wound dressing for bacteria-infected wound healing 用于细菌感染伤口愈合的自催化羟基合成伤口敷料

IF 5.5 4区医学

Nanomedicine: Nanotechnology, Biology and Medicine Pub Date : 2023-07-01 DOI: 10.1016/j.nano.2023.102683

Pinrui Zhang MSc , Xiaomu Xu PhD , Wangmei He MSc , Hong Li PhD , Yue Huang PhD , Gang Wu PhD

{"title":"Autocatalytically hydroxyl-producing composite wound dressing for bacteria-infected wound healing","authors":"Pinrui Zhang MSc , Xiaomu Xu PhD , Wangmei He MSc , Hong Li PhD , Yue Huang PhD , Gang Wu PhD","doi":"10.1016/j.nano.2023.102683","DOIUrl":"https://doi.org/10.1016/j.nano.2023.102683","url":null,"abstract":"<div>The creation of wound dressings with low drug resistance and broad-spectrum antibacterial capability is a key topic of scientific interest. To achieve this, a bactericidal wound dressing with the capacity to autocatalytically produce hydroxyl radicals (<img>OH) was developed. The wound dressing was an electrospun PCL/gelatin/glucose composite fiber mesh (PGD) with functional iron-containing metal-organic framework (Fe-MOF) nanozymes. These functional nanozymes (G@Fe) were formed by coupling glucose oxidase (GOx) and Fe-MOF through amide bonds. These nanozymes enabled the conversion of glucose released from the PGD composite mesh into hydroxyl radicals via an autocatalytic cascade reaction to destroy bacteria. The antibacterial efficiency of wound dressings and their stimulation of tissue regeneration were assessed using a MRSA-infected skin wound infection model on the back of SD mice. The G@Fe/PGD wound dressing exhibited improved wound healing capacity and had comparable biosafety to commercial silver-containing dressings, suggesting a potential replacement in the future.</div>","PeriodicalId":396,"journal":{"name":"Nanomedicine: Nanotechnology, Biology and Medicine","volume":"51 ","pages":"Article 102683"},"PeriodicalIF":5.5,"publicationDate":"2023-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"2377480","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 5

Enhancement of sonodynamic treatment of ovarian cancer based on Pt-B-P ternary nanoparticles 基于Pt-B-P三元纳米颗粒增强卵巢癌的声动力治疗

IF 5.5 4区医学

Nanomedicine: Nanotechnology, Biology and Medicine Pub Date : 2023-07-01 DOI: 10.1016/j.nano.2023.102686

Song Yue PhD , Yirui He PhD , Min Wang PhD , Xiaozhu Liu MSc , Xiaoying Li MSc , Binyi Zhao PhD , Qiang Yi PhD , Qinke Li PhD , Qiubo Yu PhD , Zhu Yang PhD

{"title":"Enhancement of sonodynamic treatment of ovarian cancer based on Pt-B-P ternary nanoparticles","authors":"Song Yue PhD , Yirui He PhD , Min Wang PhD , Xiaozhu Liu MSc , Xiaoying Li MSc , Binyi Zhao PhD , Qiang Yi PhD , Qinke Li PhD , Qiubo Yu PhD , Zhu Yang PhD","doi":"10.1016/j.nano.2023.102686","DOIUrl":"https://doi.org/10.1016/j.nano.2023.102686","url":null,"abstract":"<div>Sonodynamic therapy (SDT) can noninvasively focus sound energy to deep tumor tissues and activate sonosensitizer (such as chlorin e6(Ce6)) to produce antitumor effects. However, due to the hypoxic microenvironment of the tumor, the effect of sonodynamic therapy is limited. In this work, we successfully synthesized Platinum-Boron-Phosphorus ternary nanoparticles (Pt-B-P NPs) for the first time to efficiently catalyze the decomposition of hydrogen peroxide (H2O2) in tumor tissues to produce sufficient oxygen (O2) and improve the effect of sonodynamic treatment of ovarian cancer. In vitro studies, we found that compared with Platinum nanoparticles (Pt NPs), Pt-B-P NPs have the significantly increased ability to catalyze the decomposition of H2O2 to produce oxygen and thus the hypoxic environment of tumor cells could be improved efficiently. Meanwhile, the bio-distribution, therapeutic effect and bio-safety of Pt-B-P NPs in vivo were evaluated using BALB/c-nu mouse model of ovarian cancer and the desired result had been achieved.</div>","PeriodicalId":396,"journal":{"name":"Nanomedicine: Nanotechnology, Biology and Medicine","volume":"51 ","pages":"Article 102686"},"PeriodicalIF":5.5,"publicationDate":"2023-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"2377481","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Ultrasound-assisted biomimetic nanobubbles for targeted treatment of atherosclerosis 超声辅助仿生纳米泡靶向治疗动脉粥样硬化

IF 5.5 4区医学

Nanomedicine: Nanotechnology, Biology and Medicine Pub Date : 2023-07-01 DOI: 10.1016/j.nano.2023.102682

Xin Hu MD , Pengxuan Zhao PhD , Jun Zhang MD, PhD , Ying Zhu MD, PhD , Wei Zhou MD, PhD , Kai Hong MD , Ruiying Sun MD , Yuxue Wang MD, PhD , Yongping Lu MD, PhD , Yani Liu MD, PhD

{"title":"Ultrasound-assisted biomimetic nanobubbles for targeted treatment of atherosclerosis","authors":"Xin Hu MD , Pengxuan Zhao PhD , Jun Zhang MD, PhD , Ying Zhu MD, PhD , Wei Zhou MD, PhD , Kai Hong MD , Ruiying Sun MD , Yuxue Wang MD, PhD , Yongping Lu MD, PhD , Yani Liu MD, PhD","doi":"10.1016/j.nano.2023.102682","DOIUrl":"https://doi.org/10.1016/j.nano.2023.102682","url":null,"abstract":"<div>Cardiovascular disease caused by atherosclerosis remains the main reason of death in the worldwide scale. Although oxidative stress plays a key role in the initiation and progression of atherosclerosis, current antioxidant drugs have limited efficacy. To resolve this problem, we constructed Nox2 siRNA-loaded nanobubbles (PNBs-siNox2) coated with platelet membranes to utilize their antioxidant stress activity and targeting effect for atherosclerosis treatment. After platelet membranes modification, the capacity of PNBs-siNox2 to target collagen, foam cells, or human umbilical vein endothelial cells (HUVECs) was significantly increased. Moreover, our study demonstrated that under ultrasonic irradiation, biomimetic nanobubbles were more effective at targeting atherosclerotic plaques and delivering genes into cells. In the present study, we provided a biomimetic gene loading strategy based on nanoplatform for noninvasive, precise and efficient therapy of atherosclerosis, which further improved the efficiency of gene transfection and effectively slowed the progression of atherosclerotic plaques when combined with ultrasound.</div>","PeriodicalId":396,"journal":{"name":"Nanomedicine: Nanotechnology, Biology and Medicine","volume":"51 ","pages":"Article 102682"},"PeriodicalIF":5.5,"publicationDate":"2023-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"2359722","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Protoporphyrin IX-loaded albumin nanoparticles reverse cancer chemoresistance by enhancing intracellular reactive oxygen species 原卟啉ix负载白蛋白纳米颗粒通过增强细胞内活性氧逆转癌症化疗耐药

IF 5.5 4区医学

Nanomedicine: Nanotechnology, Biology and Medicine Pub Date : 2023-07-01 DOI: 10.1016/j.nano.2023.102688

Xiaolin Xu PhD , Chenglong Wang PhD , Wencai Guan BSc , Fanchen Wang MSc , Xin Li MSc , Jia Yuan BSc , Guoxiong Xu MD, PhD

{"title":"Protoporphyrin IX-loaded albumin nanoparticles reverse cancer chemoresistance by enhancing intracellular reactive oxygen species","authors":"Xiaolin Xu PhD , Chenglong Wang PhD , Wencai Guan BSc , Fanchen Wang MSc , Xin Li MSc , Jia Yuan BSc , Guoxiong Xu MD, PhD","doi":"10.1016/j.nano.2023.102688","DOIUrl":"https://doi.org/10.1016/j.nano.2023.102688","url":null,"abstract":"<div>Chemoresistance is the main cause of chemotherapy failure in ovarian cancer (OC). The enhanced scavenging of reactive oxygen species (ROS) by the thioredoxin system resulted in insufficient intracellular concentrations of effective ROS, leading to chemoresistance. To induce OC cell apoptosis by enhancing intracellular ROS levels, protoporphyrin IX (PpIX) and albumin-bound PTX nanoparticles (APNP) were utilized to fabricate APNP-PpIX nanoparticles. APNP-PpIX effectively generated ROS and increased the effective ROS concentration in chemoresistant cancer cells. The in vitro and in vivo experiments confirmed the effective inhibition of APNP-PpIX on chemoresistant OC cell proliferation and tumor formation. APNP-PpIX significantly improved the effectiveness of chemotherapy and photodynamic therapy, thus providing a new approach for the clinical treatment of chemoresistant OC.</div>","PeriodicalId":396,"journal":{"name":"Nanomedicine: Nanotechnology, Biology and Medicine","volume":"51 ","pages":"Article 102688"},"PeriodicalIF":5.5,"publicationDate":"2023-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"3456751","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1