Fabrication of blended nanofibrous cardiac patch transplanted with TGF-β3 and human umbilical cord MSCs-derived exosomes for potential cardiac regeneration after acute myocardial infarction

IF 4.7 4区医学 Q1 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Nanomedicine: Nanotechnology, Biology and Medicine Pub Date : 2023-11-01 DOI:10.1016/j.nano.2023.102708

Ping Ping MD , Shasha Guan MD , Chaoxue Ning MD , Ting Yang MD , Yali Zhao MD , Pei Zhang MD , Zhitao Gao MD , Shihui Fu MD

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Abstract

Acute myocardial infarction (AMI) is a common cardiovascular condition that progressively results in heart failure. In the present study, we have designed to load transforming growth factor beta 3 (TGF-β3) and cardio potential exosomes into the blended polycaprolactone/type I collagen (PCL/COL-1) nanofibrous patch (Exo@TGF-β3@NFs) and examined its feasibility for cardiac repair. The bioactivity of the developed NFs towards the migration and proliferation of human umbilical vein endothelial cells was determined using in vitro cell compatibility assays. Additionally, Exo@TGF-β3/NFs showed up-regulation of genes involved in angiogenesis and mesenchymal differentiations in vitro. The in vivo experiments performed 4 weeks after transplantation showed that the Exo@TGF-β3@NFs had a higher LV ejection fraction and fraction shortening functions. Subsequently, it has been determined that Exo@TGF-β3@NFs significantly reduced AMI size and fibrosis and increased scar thickness. The developed NFs approach will become a useful therapeutic approach for the treatment of AMI.

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用TGF-β3和人脐带MSCs衍生的外泌体移植的混合纳米纤维心脏贴片的制备，用于急性心肌梗死后潜在的心脏再生。

急性心肌梗死（AMI）是一种常见的心血管疾病，会逐渐导致心力衰竭。在本研究中，我们设计将转化生长因子β3（TGF-β3）和有心脏潜能的外泌体加载到混合聚己内酯/I型胶原（PCL/COL-1）纳米纤维贴片中(Exo@TGF-β3@NFs），并检验其用于心脏修复的可行性。使用体外细胞相容性测定法测定了所开发的NFs对人脐静脉内皮细胞迁移和增殖的生物活性。此外，Exo@TGF-β3/NFs在体外显示出对参与血管生成和间充质分化的基因的调节作用。进行的体内实验4 移植后数周Exo@TGF-β3@NFs具有较高的左心室射血分数和分数缩短功能。随后，已确定Exo@TGF-β3@NFs显著降低AMI的大小和纤维化，并增加瘢痕厚度。所开发的NFs方法将成为治疗AMI的一种有用的治疗方法。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Nanomedicine: Nanotechnology, Biology and Medicine 医学-纳米科技

CiteScore

8.10

自引率

3.60%

发文量

104

审稿时长

4.6 months

期刊介绍： Nanomedicine: Nanotechnology, Biology and Medicine (NBM) is an international, peer-reviewed journal presenting novel, significant, and interdisciplinary theoretical and experimental results related to nanoscience and nanotechnology in the life and health sciences. Content includes basic, translational, and clinical research addressing diagnosis, treatment, monitoring, prediction, and prevention of diseases.