Colloids and Surfaces B: Biointerfaces最新文献

Chitosan nanoparticles as a targeted delivery system for anti-fibrotic microRNAs for oral submucosal fibrosis treatment

IF 5.4 2区医学

Colloids and Surfaces B: Biointerfaces Pub Date : 2025-04-01 DOI: 10.1016/j.colsurfb.2025.114657

Yung-Hsin Cheng , Hsing-Yu Chen , Koichi Kato , Kai-Chiang Yang

{"title":"Chitosan nanoparticles as a targeted delivery system for anti-fibrotic microRNAs for oral submucosal fibrosis treatment","authors":"Yung-Hsin Cheng , Hsing-Yu Chen , Koichi Kato , Kai-Chiang Yang","doi":"10.1016/j.colsurfb.2025.114657","DOIUrl":"10.1016/j.colsurfb.2025.114657","url":null,"abstract":"<div><div>Oral submucous fibrosis (OSF) is characterized by excessive extracellular matrix (ECM) deposition. Dysregulation of microRNAs (miRs) is involved in the progression of OSF, and miR manipulation could be a promising therapeutic approach. Nanoformulation can protect exogenous miRs against nuclease degradation and enhance cell retention. Accordingly, chitosan (CS), which possesses an anti-fibrotic capacity, is proposed to encapsulate miRs as nanoparticles (NPs) for treating OSF. miR-negative control (miR-NC)/CS NPs were fabricated by an ionic gelation method and characterized. Human oral submucosal fibroblasts were first subjected to arecoline stimulation to induce myofibroblast differentiation and were then transfected with a miR-145 inhibitor or miR-424 inhibitor using CS NPs. For CS NPs loaded with miR-NC, the particle size was 121.9 ± 0.1 nm with a polydispersity index of 0.162 ± 0.004 and zeta potential of + 22.4 ± 0.5 mV. Transfection of these two miRs downregulated mRNA levels of transforming growth factor beta 1, actin alpha 2 smooth muscle, collagen type I alpha 1 chain (<em>COL1A1</em>), <em>COL3A1</em>, <em>COL4A1</em>, matrix metalloproteinase 2, tissue inhibitor of metalloproteinase 2, and zinc finger E-box binding homeobox 1 in myofibroblasts. A Western blot analysis revealed that miR/CS NP transfection decreased alpha-smooth muscle actin and type 1 collagen protein products. Furthermore, the wound closure ability of stimulated cells was inhibited upon transfection. In conclusion. CS NPs are a good delivery vehicle for miR transfection. Transfection of a miR-145 inhibitor and miR-424 inhibitor inhibited the TGF-β signaling pathway and decreased ECM component production, and could thus be a promising treatment for OSF.</div></div>","PeriodicalId":279,"journal":{"name":"Colloids and Surfaces B: Biointerfaces","volume":"252 ","pages":"Article 114657"},"PeriodicalIF":5.4,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143760394","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Bionic menisci with integrated material–structure–functionality for gouty arthritis

IF 5.4 2区医学

Colloids and Surfaces B: Biointerfaces Pub Date : 2025-04-01 DOI: 10.1016/j.colsurfb.2025.114672

Cijun Shuai , Shun Hu , Kangdong Wang , Gao Pan , Shuping Peng , Shengli Mi , Qi Zhong

{"title":"Bionic menisci with integrated material–structure–functionality for gouty arthritis","authors":"Cijun Shuai , Shun Hu , Kangdong Wang , Gao Pan , Shuping Peng , Shengli Mi , Qi Zhong","doi":"10.1016/j.colsurfb.2025.114672","DOIUrl":"10.1016/j.colsurfb.2025.114672","url":null,"abstract":"<div><div>Gouty arthritis may cause meniscal damage, necessitating surgery for meniscus replacement. However, the meniscus remains at risk of urate crystal accumulation and oxidative stress post-surgery. To tackle this challenge, we developed tri-level bionic menisci that achieve an integrated material-structure-functionality design. Its gradient network mimics natural structures, providing biomechanical adaptation and functioning as a porous catalyst carrier to load multifunctional nanozymes to eliminate excess uric acid and oxyradicals. Specifically, we utilized 3D printing to incorporate Pt-CeO<sub>2</sub> nanozymes within the meniscus scaffold and designed a bionic structure driven by biomechanical data of the articular cavity. The Pt-CeO<sub>2</sub> demonstrated significant catalytic efficacy in promoting the oxidation of uric acid while concurrently scavenging reactive oxygen and nitrogen species. DFT+U calculations revealed the synergistic catalytic mechanism at Pt-O<sub>v</sub>-Ce sites. We elucidated the catalytic therapeutic mechanism of the bionic menisci. Furthermore, we developed a regulatory model capable of modulating intra-articular uric acid concentration to accommodate the varied requirements of different patients. These findings underscore the potential of the bionic menisci in personalized treatment for gouty arthritis.</div></div>","PeriodicalId":279,"journal":{"name":"Colloids and Surfaces B: Biointerfaces","volume":"252 ","pages":"Article 114672"},"PeriodicalIF":5.4,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143760494","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Enhanced skin adhesion of transdermal drug delivery system via a semi-IPN strategy: in vitro/in vivo evaluation and drug release mechanism

IF 5.4 2区医学

Colloids and Surfaces B: Biointerfaces Pub Date : 2025-04-01 DOI: 10.1016/j.colsurfb.2025.114662

Guixue Chen , Maojian Li , Yanan Liu , Man Li , Daoxuan Xie , Yimeng Zhang , Nanxi Zhao , Zheng Luo

{"title":"Enhanced skin adhesion of transdermal drug delivery system via a semi-IPN strategy: in vitro/in vivo evaluation and drug release mechanism","authors":"Guixue Chen , Maojian Li , Yanan Liu , Man Li , Daoxuan Xie , Yimeng Zhang , Nanxi Zhao , Zheng Luo","doi":"10.1016/j.colsurfb.2025.114662","DOIUrl":"10.1016/j.colsurfb.2025.114662","url":null,"abstract":"<div><div>Pressure-sensitive adhesives (PSAs) used for transdermal patches often present mechanical issues that lead to cold flow phenomena, patch shifting, and disrupt dose precision while creating an unwanted \"dark ring\" effect. Semi-interpenetrating network (semi-IPN) PSAs were synthesized in this study, which enhanced cohesion and reduced cold flow by integrating a cross-linked network into a linear polymer matrix to enhance structural integrity. Meanwhile, rivastigmine, etodolac, ketoprofen, propranolol, pirfenidone, and zolmitriptan were used to evaluate drug release rates from the semi-IPN PSA. The skin adhesion properties of the semi-IPN PSA were outstanding. Its cohesion was significantly higher than two commercially available PSAs: over 1000-fold greater than DURO-TAK® 87–2287 and 75.9 times greater than DURO-TAK® 87–4098. Drug release rates were comparable between traditional and semi-IPN PSAs. The release rates of different drugs were negatively correlated with drug polarizability, suggesting dipole-dipole interactions as the key mechanism. In summary, the semi-IPN strategy offers a robust solution for high-performance transdermal patches by improving adhesion without compromising drug release.</div></div>","PeriodicalId":279,"journal":{"name":"Colloids and Surfaces B: Biointerfaces","volume":"252 ","pages":"Article 114662"},"PeriodicalIF":5.4,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143746959","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

PCA-assisted direct diagnosis of Aβ proteins for Alzheimer's disease using non-metallic SERS platform of graphitic carbon nitride@metal-organic framework

IF 5.4 2区医学

Colloids and Surfaces B: Biointerfaces Pub Date : 2025-03-31 DOI: 10.1016/j.colsurfb.2025.114665

Wenting Lan , Jialong Zhao , Xuesong Zhai , Chenjie Gu , Tao Jiang , Jianyong Wang

{"title":"PCA-assisted direct diagnosis of Aβ proteins for Alzheimer's disease using non-metallic SERS platform of graphitic carbon nitride@metal-organic framework","authors":"Wenting Lan , Jialong Zhao , Xuesong Zhai , Chenjie Gu , Tao Jiang , Jianyong Wang","doi":"10.1016/j.colsurfb.2025.114665","DOIUrl":"10.1016/j.colsurfb.2025.114665","url":null,"abstract":"<div><div>Here, we explored a non-metallic surface enhanced Raman scattering (SERS) platform based on graphitic carbon nitride@metal-organic framework (g-C<sub>3</sub>N<sub>4</sub>@MOF) for the sensitive direct diagnosis of Aβ proteins, assisted by principal component analysis (PCA). By systematically optimizing the deposition voltage and time, we successfully achieved a uniform coating of g-C<sub>3</sub>N<sub>4</sub> nanosheets over a large-area copper foil during the initial electrodeposition step. Subsequently, a homogeneous layer of flower-like MOF structures was deposited onto the g-C<sub>3</sub>N<sub>4</sub> nanosheets through a secondary electrodeposition process. This cooperation of g-C<sub>3</sub>N<sub>4</sub> nanosheets and flower-like MOF not only significantly enlarged the effective area for molecular enrichment but also promoted charge transfer through energy-level matching between the two materials. The characteristic SERS spectra of Aβ40 and Aβ42, enhanced by the g-C<sub>3</sub>N<sub>4</sub>@MOF composite substrate, were recorded and classified using PCA to extract informative features of these important biomarkers. This research exploits a new avenue for the clinical assay of neurodegenerative diseases by extracting informative features of key biomarkers.</div></div>","PeriodicalId":279,"journal":{"name":"Colloids and Surfaces B: Biointerfaces","volume":"252 ","pages":"Article 114665"},"PeriodicalIF":5.4,"publicationDate":"2025-03-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143738326","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Decellularized human amniotic member hydrogel promotes limbal stem cells proliferation

IF 5.4 2区医学

Colloids and Surfaces B: Biointerfaces Pub Date : 2025-03-29 DOI: 10.1016/j.colsurfb.2025.114656

Yongyao Tan , Wei Wang , Lingjuan Xu , Xiao Zhou , Jiachao Shen , Tianyu Zhou , Chengen Duan , Xuying Wang , Zibin Liu , Mingwu Wang , Guigang Li

{"title":"Decellularized human amniotic member hydrogel promotes limbal stem cells proliferation","authors":"Yongyao Tan , Wei Wang , Lingjuan Xu , Xiao Zhou , Jiachao Shen , Tianyu Zhou , Chengen Duan , Xuying Wang , Zibin Liu , Mingwu Wang , Guigang Li","doi":"10.1016/j.colsurfb.2025.114656","DOIUrl":"10.1016/j.colsurfb.2025.114656","url":null,"abstract":"<div><div>Allogeneic cultured limbal epithelial stem cell transplantation has shown variable clinical success in treating limbal stem cell deficiency, low success cases are likely due to insufficient stem cell quantity or functional impairment. In this study, we engineered a decellularized amniotic membrane hydrogel (dAM-gel) using a freeze-thaw protocol designed to retain extracellular matrix integrity. Post-processing, collagen content decreased modestly from 313.50 ± 27.89 μg/mg to 284.8 ± 14.82 μg/mg (<em>P</em> = 0.08), while glycosaminoglycan levels shifted from 7.20 ± 1.66 μg/mg to 6.28 ± 0.55 μg/mg (<em>P</em> = 0.27). Crucially, the protocol achieved near-complete DNA removal (7.41 ± 0.78 μg/mg vs. 0.14 ± 0.06 μg/mg) (<em>P</em> < 0.0001), ensuring minimal immunogenicity. Although the dAM-gel demonstrates limited gelation capacity at lower concentrations, it achieves robust gelation at 14 mg/ml, completing the process within 28.26 ± 1.21 minutes. Furthermore, dAM-gel facilitates the migration and proliferation of limbal stem cells, particularly p63 + cells, which are known to correlate with the success of clinical treatments. A plausible explanation for this phenomenon is that dAM-gel contains a high concentration of agrin, which facilitates the proliferation of limbal stem cells while preserving their stemness via the Yap1-cyclin D1 signaling pathway. In conclusion, dAM-gel derived from amniotic membrane presents therapeutic promise for treating limbal stem cell deficiency by enhancing the proliferation of limbal stem cells while maintaining their stem cell phenotype.</div></div>","PeriodicalId":279,"journal":{"name":"Colloids and Surfaces B: Biointerfaces","volume":"252 ","pages":"Article 114656"},"PeriodicalIF":5.4,"publicationDate":"2025-03-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143738324","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Tannic acid-based bio-MOFs with antibacterial and antioxidant properties acquiring non-hemolytic and non-cytotoxic characteristics

IF 5.4 2区医学

Colloids and Surfaces B: Biointerfaces Pub Date : 2025-03-29 DOI: 10.1016/j.colsurfb.2025.114669

Nurettin Sahiner , Olgun Guven , Sahin Demirci , Selin S. Suner , Mehtap Sahiner , Betul Ari , Mehmet Can

{"title":"Tannic acid-based bio-MOFs with antibacterial and antioxidant properties acquiring non-hemolytic and non-cytotoxic characteristics","authors":"Nurettin Sahiner , Olgun Guven , Sahin Demirci , Selin S. Suner , Mehtap Sahiner , Betul Ari , Mehmet Can","doi":"10.1016/j.colsurfb.2025.114669","DOIUrl":"10.1016/j.colsurfb.2025.114669","url":null,"abstract":"<div><div>Tannic acid (TA) based bio-metal phenolic networks (bio-MPNs) were prepared by using Cu(II), Zn(II), Bi(III), Ce(III), La(III), and Ti(IV) metal ions. TA-based bio-MPNs exhibited wedge-shaped pores between 16.4 and 25.8 nm pore size ranges. The higher gravimetric yield% was achieved for TA-Bi(III), and TA-Ti(IV) bio-MPNs with more than 90 %, and higher surface area was observed for TA-La(IIII) bio-MPNs as 56.2 m<sup>2</sup>/g with 17.3 nm average pore sizes. All TA-based MPNs are non-hemolytic with less than 5 % hemolysis ratio, whereas TA-based Bio-MPNs do not affect blood clotting with > 90 % blood clotting indexes except for TA-Cu(II) Bio-MPNs at 0.1 mg/mL concentration. Moreover, TA-Bi(III) and TA-Ce(III) Bio-MPNs were found to be safer materials showing no significant toxicity on L929 fibroblast cells at 100 μg/mL concentration, along with TA-based Bio-MPNs prepared with Cu(II), Zn(II), La(III), and Ti(IV) metal ions that could be safely used in <em>in vivo</em> applications at 1 μg/mL concentration. It has been proven by 2 different antioxidant tests that the prepared TA-based Bio-MPNs show antioxidant properties even if their TA-derived antioxidant properties decrease. Furthermore, all types of TA-based Bio-MPNs show great antimicrobial activity depending on the metal ion or microorganism types and the highest antibacterial/antifungal effect was determined for TA-Cu(II), and TA-Zn(II) Bio-MPNs with the lowest MBC/MFC values against <em>Pseudomonas aeruginosa</em> ATCC 10145, <em>Bacillus subtilis</em> ATCC 6633, and <em>Candida albicans</em> ATCC 10231<em>.</em></div></div>","PeriodicalId":279,"journal":{"name":"Colloids and Surfaces B: Biointerfaces","volume":"252 ","pages":"Article 114669"},"PeriodicalIF":5.4,"publicationDate":"2025-03-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143738325","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Recent advances in biomimetic nanodelivery systems for the treatment of glioblastoma

IF 5.4 2区医学

Colloids and Surfaces B: Biointerfaces Pub Date : 2025-03-29 DOI: 10.1016/j.colsurfb.2025.114668

Zhenru Yuan, Jing Li, Qi Na

{"title":"Recent advances in biomimetic nanodelivery systems for the treatment of glioblastoma","authors":"Zhenru Yuan, Jing Li, Qi Na","doi":"10.1016/j.colsurfb.2025.114668","DOIUrl":"10.1016/j.colsurfb.2025.114668","url":null,"abstract":"<div><div>Glioblastoma remain one of the deadliest malignant tumors in the central nervous system, largely due to their aggressiveness, high degree of heterogeneity, and the protective barrier of the blood-brain barrier (BBB). Conventional therapies including surgery, chemotherapy and radiotherapy often fail to improve patient prognosis due to limited drug penetration and non-specific toxicity. We then present recent advances in biomimetic nanodelivery systems, focusing on cell membrane coatings, nanoenzymes, and exosome-based carriers. By mimicking endogenous biological functions, these systems demonstrate improved immune evasion, enhanced BBB traversal, and selective drug release within the tumor microenvironment. Nevertheless, we acknowledge unresolved bottlenecks related to large-scale production, stability, and the intricacies of regulatory compliance. Looking forward, we propose an interdisciplinary roadmap that combines materials engineering, cellular biology, and clinical expertise. Through this collaborative approach, this work aims to optimize biomimetic nanodelivery for glioma therapy and ultimately improve patient outcomes.</div></div>","PeriodicalId":279,"journal":{"name":"Colloids and Surfaces B: Biointerfaces","volume":"252 ","pages":"Article 114668"},"PeriodicalIF":5.4,"publicationDate":"2025-03-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143738323","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Exploring glutathione-decorated micelles for drug delivery: A promise for enhanced cellular uptake

IF 5.4 2区医学

Colloids and Surfaces B: Biointerfaces Pub Date : 2025-03-28 DOI: 10.1016/j.colsurfb.2025.114664

Martyna Truszkowska , Elsa Reisenberger , Gergely Kali , Daniel Stengel , Ahmad Saleh , Anna Seybold , Tobias Kipura , Marcel Kwiatkowski , Andreas Bernkop-Schnürch

{"title":"Exploring glutathione-decorated micelles for drug delivery: A promise for enhanced cellular uptake","authors":"Martyna Truszkowska , Elsa Reisenberger , Gergely Kali , Daniel Stengel , Ahmad Saleh , Anna Seybold , Tobias Kipura , Marcel Kwiatkowski , Andreas Bernkop-Schnürch","doi":"10.1016/j.colsurfb.2025.114664","DOIUrl":"10.1016/j.colsurfb.2025.114664","url":null,"abstract":"<div><div>This study aimed to evaluate the effect of thiolated micelles on the cellular uptake of their payload.</div><div>Reduced and oxidized glutathione were covalently attached to palmitic acid via amide bond formation. Micelles formed with these thiolated surfactants (M<sub>P-GSH</sub> and M<sub>P-GSSG-P</sub>) were evaluated regarding critical micellar concentration (CMC) and hemolytic activity. Cytotoxicity was evaluated on HEK 293 and HeLa cells. Diffusion of micelles in these cells was evaluated by fluorescence correlation spectroscopy (FCS). Furthermore, cellular uptake of micelles containing coumarin-6 as model drug was analyzed by flow cytometry and confocal laser scanning microscopy.</div><div>CMC of M<sub>P-GSSG-P</sub>, M<sub>P-GSH</sub>, and palmitic acid micelles (M<sub>PA</sub>) was determined to be 0.455 mM, 0.166 mM, and 0.046 mM, respectively. At a concentration of 0.5 % M<sub>P-GSSG-P,</sub> M<sub>P-GSH</sub>, and M<sub>PA</sub> caused around 80 % hemolysis. In HEK cells, M<sub>P-GSSG-P</sub> exhibited toxicity at a concentration of 0.25 %, while M<sub>P-GSH</sub> showed toxicity at 0.06 % after 4 hours of incubation. In contrast, HeLa cells were more resilient, with only M<sub>P-GSH</sub> showing toxicity at 0.25 %. Diffusivity of M<sub>P-GSH</sub> and M<sub>P-GSSG-P</sub> within the cells was higher than that of M<sub>PA</sub>. Cellular uptake studies demonstrated a significantly (p < 0.05) enhanced internalization of M<sub>P-GSH</sub> and M<sub>P-GSSG-P</sub>, that was 112-fold and 270-fold higher in HEK 293 cells and 12-fold and 60-fold higher in HeLa cells when compared to M<sub>PA</sub>.</div><div>These findings suggest that M<sub>P-GSH</sub> and M<sub>P-GSSG-P</sub> could serve as promising vehicles for enhancing cellular uptake of drugs.</div></div>","PeriodicalId":279,"journal":{"name":"Colloids and Surfaces B: Biointerfaces","volume":"252 ","pages":"Article 114664"},"PeriodicalIF":5.4,"publicationDate":"2025-03-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143746958","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Immuno-osteoinductive 3D printed hydrogel scaffolds with triple crosslinking and GA/EGCG release for bone healing 用于骨愈合的具有三重交联和 GA/EGCG 释放功能的免疫骨诱导性三维打印水凝胶支架

IF 5.4 2区医学

Colloids and Surfaces B: Biointerfaces Pub Date : 2025-03-27 DOI: 10.1016/j.colsurfb.2025.114651

Yanlan Yang , Yang Xiang , Pu Xu , Wenbo Zhang , Yawen Wang , Longbao Feng , Rong She

{"title":"Immuno-osteoinductive 3D printed hydrogel scaffolds with triple crosslinking and GA/EGCG release for bone healing","authors":"Yanlan Yang , Yang Xiang , Pu Xu , Wenbo Zhang , Yawen Wang , Longbao Feng , Rong She","doi":"10.1016/j.colsurfb.2025.114651","DOIUrl":"10.1016/j.colsurfb.2025.114651","url":null,"abstract":"<div><div>Bone defects, caused by trauma, osteomyelitis, or osteoporosis, represent a significant global health challenge in orthopedics. However, current bone repair strategies often neglect the critical role of the immune microenvironment, which can impede effective bone regeneration. To address this gap, we developed a 3D-printed triple crosslinked hydrogel scaffold incorporating slow-release glycopyrrolate (GA) and epigallocatechin gallate (EGCG), that it could promote bone regeneration by modulating the immune response. We evaluated their immunomodulatory and bone-regenerative effects through in vitro cellular experiments and rat cranial defect models. Results demonstrated that these scaffolds effectively modulated the immune microenvironment, reducing inflammation while promoting osteoblast differentiation and proliferation, thereby significantly enhancing new bone formation and density. In conclusion, our novel 3D-printed hydrogel scaffold offers a promising approach to bone defect repair through its unique combination of mechanical strength, immunomodulation, and osteogenesis. This study provides valuable insights into leveraging immunomodulatory agents for enhanced bone regeneration, highlighting potential clinical applications.</div></div>","PeriodicalId":279,"journal":{"name":"Colloids and Surfaces B: Biointerfaces","volume":"252 ","pages":"Article 114651"},"PeriodicalIF":5.4,"publicationDate":"2025-03-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143735021","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Magnetotactic bacteria-mediated integrated magnetic targeted hyperthermia for in-situ deep-seated tumor

IF 5.4 2区医学

Colloids and Surfaces B: Biointerfaces Pub Date : 2025-03-27 DOI: 10.1016/j.colsurfb.2025.114658

Qingmeng Wang , Changyou Chen , Haoyu Zhao , Yangkun Jiao , Haitao Chen , Pingping Wang , Tao Song

{"title":"Magnetotactic bacteria-mediated integrated magnetic targeted hyperthermia for in-situ deep-seated tumor","authors":"Qingmeng Wang , Changyou Chen , Haoyu Zhao , Yangkun Jiao , Haitao Chen , Pingping Wang , Tao Song","doi":"10.1016/j.colsurfb.2025.114658","DOIUrl":"10.1016/j.colsurfb.2025.114658","url":null,"abstract":"<div><div>Unlike hyperthermia after intratumoral injection, the method of integrated magnetic targeted hyperthermia (iMTH) guides magnetic medium to the target site and then directly performs <em>in-situ</em> heating, showing great potential for effective treatment of deep-seated tumors in the body. Magnetotactic bacteria (MTB), having chain-like arranged magnetic nanoparticles within its body and active movement along an external magnetic field, are considered as a very fitted material for iMTH. However, the amount of MTB concentrated on the deep-seated tumor posed a significant challenge for the successful implementation of iMTH. Herein, we aim to validate the strategy of integrating magnetic targeting and hyperthermia. An <em>in-situ</em> liver tumor model in mouse was developed as deep-seated tumors. After administering the polar MTB MO-1 intravenously via the tail vein, a focusing magnetic field navigated these bacteria to effectively accumulate at the deep-seated tumor site. Immediately afterwards, this targeted aggregation of MO-1 cells triggered a localized magnetic hyperthermia directly at the cancer site under an applied alternating magnetic field. Our findings demonstrated that this hyperthermia induced by the bacteria led to the death of liver cancer cells, thereby effectively curbing the progression and growth of the cancer. These promising results suggested that an iMTH approach was developed, harnessing the power of MTB. This method stands as an exciting and potential therapeutic strategy for the treatment of deep-seated tumors, offering new hope in the fight against cancer.</div></div>","PeriodicalId":279,"journal":{"name":"Colloids and Surfaces B: Biointerfaces","volume":"252 ","pages":"Article 114658"},"PeriodicalIF":5.4,"publicationDate":"2025-03-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143738322","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0