Colloids and Surfaces B: Biointerfaces最新文献

{"title":"H2O2 self-supplying cascade catalytic nanoreactors amplify oxidative stress for augmented cuproptosis-driven multimodal synergistic therapy of breast cancer","authors":"Zhe Wen ,&nbsp;Rui-Rui Zhao ,&nbsp;Xiao Wu,&nbsp;Chun-Lei Liu,&nbsp;Chun-Zhao Liu","doi":"10.1016/j.colsurfb.2025.114802","DOIUrl":"10.1016/j.colsurfb.2025.114802","url":null,"abstract":"<div><div>Cuproptosis, a newly identified form of programmed cell death, has exhibited great potential in the treatment of breast cancer. However, excess glutathione and inadequate hydrogen peroxide (H₂O₂) and poor Fenton reaction efficiency in tumor cells limit cuproptosis-photo-chemodynamic therapy effect. Herein, we developed a H₂O₂ self-supplying cascade catalytic self-assembled nanoreactors (PGIC NPs) based on chemotherapeutic drug paclitaxel (PTX), natural enzyme glucose oxidase (GOx), metal copper ions (Cu²⁺) and photosensitizer indocyanine green (ICG) for cuproptosis-mediated chemo-photo-starvation-chemodynamic therapy. These multifunctional self-assembled nanoreactors with ideal particle size were more easily taken up by 4T1 cells. PGIC NPs could catalyze tumor-overexpressed glucose to achieve the self-supplying of H₂O₂ to promote starvation therapy, cascade catalyze hydroxyl radical (·OH) production via Fenton-like reaction to facilitate chemodynamic therapy. These nanoparticles could not only consume GSH, induce a photothermal effect to strengthen the efficiency of Fenton-like reactors, promote the production of ·OH and singlet oxygen (¹O₂) to create a “ROS storm”, augment oxidative stress; but also induce acylated protein oligomerization to trigger cuproptosis, leading to cuproptosis-driven chemo-photo-chemodynamic-starvation synergistic therapy. <em>In vivo</em> studies demonstrated that PGIC NPs showed good biosafety and could significantly inhibit the growth of 4T1 tumor-bearing mice. Overall, this study provided new insights into H₂O₂ self-supplying cascade catalytic self-assembled nanoreactors to achieve cuproptosis-driven multimodal synergistic therapy of breast cancer.</div></div>","PeriodicalId":279,"journal":{"name":"Colloids and Surfaces B: Biointerfaces","volume":"254 ","pages":"Article 114802"},"PeriodicalIF":5.4,"publicationDate":"2025-05-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144072079","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Synergistic anti-tumor effects of mRNA vaccine and PERK inhibitor combination in melanoma treatment","authors":"Xiaolong Li ,&nbsp;Lanlan Ma ,&nbsp;Jueshuo Guo,&nbsp;Yaya Wei,&nbsp;Shijie Ma,&nbsp;Yaping Mai,&nbsp;Guojing Gou,&nbsp;Wenbao Zuo,&nbsp;Jianhong Yang","doi":"10.1016/j.colsurfb.2025.114808","DOIUrl":"10.1016/j.colsurfb.2025.114808","url":null,"abstract":"<div><div>Melanoma is a highly aggressive form of skin cancer. mRNA vaccines deliver genetic material encoding specific antigens into cells, thereby triggering the host immune system to produce the antigen. Gp-100, an antigenic protein expressed on the surface of melanoma cells, serves as a target mRNA to stimulate the cytotoxic T lymphocyte (CTL) response. However, the absence of natural killer (NK) cells can lead to significant tumor cell proliferation. Gardiquimod, a TLR7 agonist, enhances NK cell cytotoxicity, promoting tumor clearance. In advanced melanoma, the unfolded protein response (UPR) often becomes dysregulated. By inhibiting protein kinase R-like ER kinase (PERK), the UPR can be disrupted, inducing apoptosis in cancer cells and shifting the tumor microenvironment (TME) towards an increased M1/M2 macrophage ratio. This study developed a cationic liposome-based mRNA vaccine (GD-LPR) using DOTMA to co-deliver gp-100 mRNA and the TLR7 agonist Gardiquimod, combined with the PERK inhibitor GSK2656157 (GSK), for synergistic melanoma immunotherapy. GD-LPR achieved 95 % mRNA encapsulation efficiency and demonstrated enhanced dendritic cell maturation and NK cell activation both in vitro and in vivo. In subcutaneous melanoma models, GD-LPR+GSK reduced tumor volume and prolonged survival by modulating the tumor microenvironment (TME): increasing CD8⁺ T cells (Fig. 3 f), repolarizing M2 to M1 macrophages (Fig. 4 f), and suppressing IL-10 while elevating pro-inflammatory cytokines (IL-2, IFN-γ, TNF-α). Mechanistically, GSK inhibited PERK/ATF-4 signaling, synergizing with GD-LPR to suppress lung metastasis. The combination of the GD-LPR vaccine and GSK provides new potential strategies for treating melanoma, particularly in subcutaneous tumors and lung metastases.</div></div>","PeriodicalId":279,"journal":{"name":"Colloids and Surfaces B: Biointerfaces","volume":"254 ","pages":"Article 114808"},"PeriodicalIF":5.4,"publicationDate":"2025-05-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144071915","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Short peptide hydrogel with angular structure for hydrophobic antitumor drug delivery and controlled release","authors":"Qingqing Yao ,&nbsp;Jie Gao ,&nbsp;Linsheng Liu ,&nbsp;Jinfang Shi ,&nbsp;Hajra Zafar ,&nbsp;Muhammad Ijaz Khan ,&nbsp;Jianguo Zhu ,&nbsp;Faisal Raza ,&nbsp;Ying Zhu","doi":"10.1016/j.colsurfb.2025.114793","DOIUrl":"10.1016/j.colsurfb.2025.114793","url":null,"abstract":"<div><div>Although hydrophobic anti-tumor drugs such as paclitaxel (PTX) have been used to treat various cancers, their clinical application is limited due to their poor water solubility, low bioavailability and adverse drug reactions. Peptide hydrogels are being increasingly used for antitumor drug delivery due to their diverse synthesis and function and excellent biocompatibility. From the perspective of economic and clinical benefits, it is essential to design peptide hydrogels for anti-tumor drug delivery that can achieve tumor microenvironment responsiveness with short sequences. We designed a short peptide, KK (KIKI^DPPIKIK), consisting of 10 amino acids with a corner structure. The angular structure of KK is conducive to forming a network structure under neutral conditions for PTX inclusion. The drug-loaded short peptide hydrogel delivered PTX to the tumor site by injection and continuously released the drug under slight acidic stimulation of the tumor. The short peptide was synthesized by solid-phase peptide synthesis and verified by in vitro release experiments as a carrier for the delivery of hydrophobic antitumor drugs. Electron transmission microscopy was used to observe the microstructure differences of the short peptides under neutral and acidic conditions. Circular dichroism revealed the difference of secondary structure of the peptide under neutral and acidic conditions. The injectability of the short peptide hydrogel was verified by rheological experiments. The cytotoxicity in vitro and anti-tumor effect in vivo showed that the drug-loaded short peptide hydrogel could improve the anti-tumor effect. The biological safety of the short peptide hydrogel was confirmed by cell biocompatibility in vitro and in vivo. In summary, the pH-sensitive peptide can form a stable drug-loaded hydrogel in vitro, enabling sustained release of PTX upon injection into tumor tissue, thereby achieving long-term therapeutic efficacy with reduced toxic side effects. Moreover, it is noteworthy that the functional properties of this peptide can be achieved with just 10 amino acids, resulting in decreased synthetic costs and difficulties while maximizing clinical benefits.</div></div>","PeriodicalId":279,"journal":{"name":"Colloids and Surfaces B: Biointerfaces","volume":"254 ","pages":"Article 114793"},"PeriodicalIF":5.4,"publicationDate":"2025-05-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144071913","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Unlocking the secrets: Structure-function dynamics of plant proteins","authors":"Tanweer Haider ,&nbsp;Wasim Akram ,&nbsp;Ramakant Joshi ,&nbsp;Monika Vishwakarma ,&nbsp;Shivani Saraf ,&nbsp;Vandana Soni ,&nbsp;Navneet Garud","doi":"10.1016/j.colsurfb.2025.114791","DOIUrl":"10.1016/j.colsurfb.2025.114791","url":null,"abstract":"<div><div>Plant-based proteins are becoming essential resources for sustainable food systems, pharmaceutical innovations, and functional materials. This review examines the complex structure-function relationships of plant proteins, emphasising their crucial role in defining functional properties and applications. The primary structure, consisting of amino acid sequences, along with secondary, tertiary, and quaternary structures, profoundly affects protein behaviour. External factors such as pH, ionic strength, temperature, and processing techniques like extrusion and enzymatic modification can influence protein structure, consequently modifying their functional properties. Consider rewording to \"Advanced processing techniques, such as high-pressure and non-thermal methods, effectively refine protein structures while preserving their functionality.Computational modelling, employing molecular dynamics and artificial intelligence, is proposed as a revolutionary instrument for forecasting and enhancing structure-function relationships. An emerging application of plant proteins is targeted drug delivery, whose structural characteristics facilitate accurate encapsulation and release of therapeutic agents. Case studies highlight the importance of protein surface characteristics in attaining precise cellular or tissue targeting, especially for conditions related to cancer and inflammation. This review concludes by highlighting strategic avenues for harnessing the complete potential of plant proteins, placing them at the cutting edge of innovation in food science, biotechnology, and drug delivery.</div></div>","PeriodicalId":279,"journal":{"name":"Colloids and Surfaces B: Biointerfaces","volume":"254 ","pages":"Article 114791"},"PeriodicalIF":5.4,"publicationDate":"2025-05-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144071916","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Synthesis and study on treatment mechanism of galactosyl metalloporphyrin targeting hepatocarcinoma","authors":"Wei Hu ,&nbsp;Jie Zhou ,&nbsp;Jing Huang ,&nbsp;Yanhua Yu","doi":"10.1016/j.colsurfb.2025.114794","DOIUrl":"10.1016/j.colsurfb.2025.114794","url":null,"abstract":"<div><div>Tetra-antennary galactosyl metalloporphyrin ZnTPPGal targeting hepatocarcinoma was synthesized. ZnTPPGal exhibited excellent fluorescent properties and high mortality (IC₅₀ 0.91 × 10⁻⁵ M ± 2.59) to HepG2 cells, showing good potential in drug tracing and targeted therapy. Relative quantitation proteomics analysis revealed that ZnTPPGal induced 475 differentially expressed proteins (DEPs) including 174 upregulated and 301 downregulated (Ratio &gt; 1.5 or &lt; 0.67). ZnTPPGal could inhibit cell survival and activate apoptosis mainly through endoplasmic reticulum stress pathway and mitochondrial dependent pathway, while did not activate autophagy. Functional proteins of EGFR, ASK1, Cat D, Rho, JNK, RagB and Rheb could be used as potential candidate proteins for hepatocarcinoma treatment and drug development.</div></div>","PeriodicalId":279,"journal":{"name":"Colloids and Surfaces B: Biointerfaces","volume":"254 ","pages":"Article 114794"},"PeriodicalIF":5.4,"publicationDate":"2025-05-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144071914","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Corona-like nanostructures: Quantum dots meet pleural fluid proteins/peptides for theranostic applications","authors":"Kerem Tok ,&nbsp;Faezeh Ghorbanizamani ,&nbsp;Hichem Moulahoum ,&nbsp;Firat Baris Barlas ,&nbsp;Emine Guler Celik ,&nbsp;Dilara Gürsoy ,&nbsp;Rza Memmedov ,&nbsp;Tevfik Ilker Akcam ,&nbsp;Kutsal Turhan ,&nbsp;Figen Zihnioglu ,&nbsp;Suna Timur","doi":"10.1016/j.colsurfb.2025.114792","DOIUrl":"10.1016/j.colsurfb.2025.114792","url":null,"abstract":"<div><div>The incorporation of protein and peptide components into nanoparticles is a revolutionary advancement in nanotheranostics, particularly in the domain of personalised medicine. This study delves into the creation of multifunctional theranostic nanoparticles by conjugating quantum dots (QDs) with proteins and peptides sourced from the pleural fluid of lung cancer patients. Our objective is to enhance the targeting and therapeutic potential of QDs through the formation of corona-like nanostructures. Pleural fluids from lung cancer patients were pooled and precipitated to enrich protein and peptide fractions. These enriched fractions, alongside untreated pooled pleural fluid, were utilized to coat QDs, forming corona-like nanostructures. Comprehensive characterization revealed robust interactions between QDs and pleural fluid proteins/peptides, resulting in heightened fluorescence and stability. Targeted in vitro assays on lung cancer cells (A549) and normal epithelial lung cells (BEAS-2B) demonstrated selective cancer cell targeting and improved therapeutic efficacy. Furthermore, combining these nanostructures with radiotherapy markedly increased cancer cell death compared to radiotherapy alone. This pioneering approach underscores the significant potential of pleural fluid-derived protein/peptide-coated QDs in developing targeted, effective multifunctional nanostructures. By leveraging the unique properties of pleural fluid proteins/peptides and QDs, this study opens new avenues for personalized medicine, poised to revolutionize cancer therapy applications.</div></div>","PeriodicalId":279,"journal":{"name":"Colloids and Surfaces B: Biointerfaces","volume":"254 ","pages":"Article 114792"},"PeriodicalIF":5.4,"publicationDate":"2025-05-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144071918","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Single-step method for the immobilization of hydroxyapatite on 3D-printed porous polyetherketoneketone implants for the enhanced cell adhesion and osteogenic differentiation","authors":"Semen Goreninskii ,&nbsp;Igor Akimchenko ,&nbsp;Alexander Vorobyev ,&nbsp;Mikhail Konoplyannikov ,&nbsp;Yuri Efremov ,&nbsp;Evgeniy Sudarev ,&nbsp;Peter Timashev ,&nbsp;Andrei Zvyagin ,&nbsp;Evgeny Bolbasov ,&nbsp;Sergei Tverdokhlebov","doi":"10.1016/j.colsurfb.2025.114777","DOIUrl":"10.1016/j.colsurfb.2025.114777","url":null,"abstract":"<div><div>Being biocompatible and extremely rigid polymer, polyetherketoneketone (PEKK) emerged as a promising material for the development of bone implants, but its inert surface limits bone-implant integration. Herein, we report a single-step approach for the immobilization of hydroxyapatite (HAp) particles on the surface of additively fabricated porous PEKK implants based on the swelling of the implant surface with subsequent entrapment of the HAp particles. By means of the scanning electron microscopy, it was established that this approach effectively preserved the morphology (pore diameter and printed line width) of the original implants. With that, up to 35.0 ± 14.0 % of the sample surface was covered with HAp particles, leading to improved hydrophilicity (&lt;1° water contact angle). From the energy-dispersive spectroscopy results, calcium and phosphorus content on the surface of the modified samples reached 17.4 ± 4.1 wt% and 8.0 ± 1.7 wt%, respectively. Compression test revealed no changes in the samples strength. From the <em>in vitro</em> experiment with bone marrow multipotent stem cells (MSC) HAp immobilization improved cell adhesion (from 121 ± 40 cells/mm² to 234 ± 8 cells/mm²) and induced their osteogenic differentiation. Thus, the proposed method may be used for the development of PEKK-based implants for bone tissue restoration.</div></div>","PeriodicalId":279,"journal":{"name":"Colloids and Surfaces B: Biointerfaces","volume":"254 ","pages":"Article 114777"},"PeriodicalIF":5.4,"publicationDate":"2025-05-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144071912","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Apatinib-loaded silicate nanoparticles coated with macrophage membranes and PD-1 antibody for enhanced chemo-immunotherapy in ovarian cancer via VEGFR2 and PD-1 dual inhibition","authors":"Hongru Li ,&nbsp;Yuhan Wang ,&nbsp;Shunqing Zhou ,&nbsp;Jianli Liu ,&nbsp;Yuemei Jin","doi":"10.1016/j.colsurfb.2025.114790","DOIUrl":"10.1016/j.colsurfb.2025.114790","url":null,"abstract":"<div><div>Ovarian cancer remains one of the most challenging malignancies to treat due to its aggressive nature and resistance to conventional therapies. In this study, we developed a nanoparticle-based system (Apa@SiO₂@MP) that combines chemotherapy with immune checkpoint inhibition for enhanced treatment of ovarian cancer. The system consists of mesoporous silica nanoparticles (SiO₂ NPs) coated with macrophage membranes (MP) and functionalized with programmed death 1 (PD-1) antibody, designed to improve the delivery and targeting of apatinib, a tyrosine kinase inhibitor. The system demonstrated effective drug encapsulation, controlled release, and stability in physiological environments. <em>In vitro</em> assays revealed that Apa@SiO₂@MP had minimal cytotoxicity in normal cells but significantly reduced cell viability in ovarian cancer cells (SKOV-3), highlighting its cancer-targeting ability. Apatinib effectively inhibited VEGFR2 expression and induced reactive oxygen species (ROS) production, further promoting anti-cancer effects. <em>In vivo</em>, Apa@SiO₂@MP treatment led to enhanced tumor inhibition, as well as significant immune response activation, including increased CD4⁺ and CD8⁺ T cells and elevated IFN-γ levels. This study provides a promising multi-modal strategy for overcoming challenges in cancer therapy by integrating chemotherapy, immunotherapy, and targeted drug delivery, offering potential for improved treatment outcomes in ovarian cancer.</div></div>","PeriodicalId":279,"journal":{"name":"Colloids and Surfaces B: Biointerfaces","volume":"254 ","pages":"Article 114790"},"PeriodicalIF":5.4,"publicationDate":"2025-05-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144071911","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Novel colchicine ethosomes cataplasm for the treatment of acute gouty arthritis","authors":"Yao-Yao Wei ,&nbsp;Yang Zhang ,&nbsp;Xue Lu ,&nbsp;Jie Zhou ,&nbsp;Huang-Wei Cheng ,&nbsp;Yuan-Yuan Liu ,&nbsp;Hua Zhang ,&nbsp;Wen Chen","doi":"10.1016/j.colsurfb.2025.114776","DOIUrl":"10.1016/j.colsurfb.2025.114776","url":null,"abstract":"<div><div>Gout is a disease caused by the deposition of sodium urate (MSU) crystals in the joints and tissues. Colchicine (COL) has become the first-line drug for the treatment of acute gout due to its low price and efficacy. However, colchicine is highly cytotoxic and oral administration is prone to cause severe adverse effects on the gastrointestinal tract, liver and kidney. Therefore, this study aimed to develop a novel dermal delivery formulation for addressing the safety concerns of this drug. The researchers used ethosomes encapsulation technology to improve the skin permeability of COL. In addition, in order to improve the performance of the ethosomes, it was screened and determined that the addition of 1.0–1.5 mg of ceramide III (Cer3) per mL of ethosomes as a modifier could significantly enhance the stability of the ethosomes, Cer3/COL-ethosomes (CCE) were successfully constructed. The CCE was then mixed with a cataplasm matrix to produce a colchicine-carrying CCE cataplasm, which demonstrated the superimposed effect of the advantages of the two dosage forms, the ethosomes and the cataplasm. Compared with the traditional delivery method of COL, this topical formulation is an attractive alternative for the treatment of gout as it can achieve effective blood levels without causing fluctuations in blood levels, and has good efficacy and higher safety profile.</div></div>","PeriodicalId":279,"journal":{"name":"Colloids and Surfaces B: Biointerfaces","volume":"254 ","pages":"Article 114776"},"PeriodicalIF":5.4,"publicationDate":"2025-05-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144068452","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Amifostine-loaded Prussian blue nanoparticles for simultaneous efficient radioprotection and deep decorporation of radiocesium","authors":"Boyan Wang,&nbsp;Yuchen Liu,&nbsp;Chengqi Li,&nbsp;Meiyun Xu,&nbsp;Daoben Hua","doi":"10.1016/j.colsurfb.2025.114788","DOIUrl":"10.1016/j.colsurfb.2025.114788","url":null,"abstract":"<div><div>Radiocesium is highly water-soluble and easily accumulates in agricultural products and seafood. Ingestion of radiocesium results in internal irradiation, significantly increasing the risk of tissue and organ damage as well as carcinogenesis. In this paper, we develop a strategy for simultaneous radioprotection and decorporation of radiocesium by amifostine-loaded Prussian blue (Am@PB) nanoparticles. The nanoparticles are prepared through chemical coordination between amine/phosphate groups of amifostine and Fe (II)/Fe (III) sites of Prussian blue (PB). Am@PB nanoparticles mitigate radiation-induced damage to peripheral blood cells and organs, improving the survival rate of irradiated mice. This is due to the synergistic effects of the nano-enzymatic activity of PB component and the high reducibility of sulfhydryl groups generated through amifostine hydrolysis by alkaline phosphatase. Furthermore, the deep excretion of cesium is achieved <em>via</em> feces along the metabolic pathway of Am@PB, leading to an enhanced decorporation efficiency of over 50 % compared to orally administered commercial PB. This work provides a design strategy for efficient radioprotective decorporation agents with potential applications in the treatment of internal radiocesium contamination.</div></div>","PeriodicalId":279,"journal":{"name":"Colloids and Surfaces B: Biointerfaces","volume":"254 ","pages":"Article 114788"},"PeriodicalIF":5.4,"publicationDate":"2025-05-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144068451","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}