Colloids and Surfaces B: Biointerfaces最新文献

{"title":"Gardeniae Fructus derived natural small molecule-based carbon dots promoting activation and aggregation of platelets to accelerate hemostasis","authors":"Jiawen Zhang ,&nbsp;Yong Hou ,&nbsp;Xueshan Xu ,&nbsp;Yihang Li ,&nbsp;Zhonghao Sun ,&nbsp;Zhaocui Sun ,&nbsp;Xudong Xu ,&nbsp;Xiaowei Huo ,&nbsp;Guoxu Ma","doi":"10.1016/j.colsurfb.2025.114601","DOIUrl":"10.1016/j.colsurfb.2025.114601","url":null,"abstract":"<div><div>Effective and prompt hemostasis poses a formidable challenge currently, especially for tissue injury-induced endogenous hemorrhage. Carbonic herbs originated from traditional Chinese medicine (TCM) have been historically utilized in the treatment of endogenous hemorrhages, but the underlying active ingredients and hemostatic mechanism still remain largely unexplored. Study on carbon dots (CDs) prepared by main small molecule components from TCM is of great significance for unravelling the mechanism of action of carbonic herbs. <em>Gardeniae Fructus</em> is a classical TCM which widely used for hemostasis after carbonization. Given that geniposide is one of main small molecule components of it, geniposide-carbon dots (Gen-CDs) are developed using modified hydrothermal method and considered as the key pharmacological agents responsible for the hemostatic efficacy of carbonized <em>Gardeniae Fructus</em> (CGF). Undergoing repeated dehydration and condensation, Gen-CDs having abundant functional groups were obtained from geniposide, exhibiting superior water solubility and bioavailability. The mouse liver injury model, tail amputation model, and rat blood-heat hemorrhage (BHH) syndrome model were all employed to confirm the excellent hemostatic activity of Gen-CDs on exogenous hemorrhage. The <em>in vitro</em> and <em>vivo</em> experiments had indicated that Gen-CDs could effectively promote platelet activation and aggregation. Overall, the successful preparation of Gen-CDs with effective and prompt hemostatic performance could provide a new perspective for elucidating the substantial basis and mechanism of carbonic herbs.</div></div>","PeriodicalId":279,"journal":{"name":"Colloids and Surfaces B: Biointerfaces","volume":"251 ","pages":"Article 114601"},"PeriodicalIF":5.4,"publicationDate":"2025-02-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143527463","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Electroacupuncture as a promising therapeutic strategy for doxorubicin-induced heart failure: Insights into the PI3K/AKT/mTOR/ULK1 and AMPK /mTOR /ULK1 pathways","authors":"Peng Chao ,&nbsp;Xueqin Zhang ,&nbsp;Lei Zhang,&nbsp;Zhengyang Han,&nbsp;Runda Jie,&nbsp;Pingxiu Duan,&nbsp;Min Cao,&nbsp;Aiping Yang","doi":"10.1016/j.colsurfb.2025.114590","DOIUrl":"10.1016/j.colsurfb.2025.114590","url":null,"abstract":"<div><h3>Background</h3><div>Electroacupuncture (EA), a traditional Chinese medicine therapy, exhibits cardioprotective and therapeutic effects against cardiac injury. However, the precise mechanisms underlying these benefits remain unclear.</div></div><div><h3>Purpose</h3><div>The aim of this study is to examine the impact of EA on Doxorubicin-Induced heart failure and elucidate the mechanisms involved.</div></div><div><h3>Methods</h3><div>C57BL/6 mice were randomly assigned to six experimental groups, including a control group, a DCM group, a DCM group receiving non-acupoint EA (NEA), and a DCM group receiving acupoint EA (EA). The cardiac function, levels of inflammatory factors, and markers of apoptosis were assessed both in vivo and in vitro. The presence of AMPK/mTOR/ULK1(Ser317) and PI3K/AKT/mTOR/ULK1(Ser757) was confirmed.</div></div><div><h3>Results</h3><div>EA stimulation significantly improved cardiac function, as evidenced by increased left ventricular ejection fraction (LVEF), E/A ratio, and fractional shortening (FS%) compared to the DCM group (p &lt; 0.05). After EA stimulation, the phosphorylation levels of PI3K/AKT increase, leading to elevated expression of mTOR/ULK1(Ser757), which ultimately inhibited the expression of apoptosis-related proteins and inflammatory factors. Simultaneously, EA stimulation could inhibit the phosphorylation levels of AMPK, reducing the expression of mTOR/ULK1(Ser317), and thereby also inhibiting the expression of apoptosis-related proteins and inflammatory factors.</div></div><div><h3>Conclusions</h3><div>This study showed that EA stimulation can counteract myocardial damage caused by apoptosis and inflammation, thereby significantly improving cardiac function and prognosis in HF mice. The mechanism may be that EA stimulation activates the PI3K/AKT/mTOR/ULK1(ser757) pathway and inhibits the AMPK/ULK1(ser317) pathway. EA stimulation exerts the same effect by regulating these two pathways in different directions, ultimately reducing myocardial cell apoptosis and cardiac inflammation.</div></div>","PeriodicalId":279,"journal":{"name":"Colloids and Surfaces B: Biointerfaces","volume":"251 ","pages":"Article 114590"},"PeriodicalIF":5.4,"publicationDate":"2025-02-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143527464","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Influence of coordination number and ionic radius on metal ion preference and activity of lanthanide-dependent alcohol dehydrogenase: Insights from mutational studies and density functional theory","authors":"Lun Wang ,&nbsp;Ke Liu ,&nbsp;Zhongdi Song ,&nbsp;Hainam Do ,&nbsp;Lirong Yang ,&nbsp;Jianping Wu ,&nbsp;Ling Jiang ,&nbsp;Haoran Yu","doi":"10.1016/j.colsurfb.2025.114596","DOIUrl":"10.1016/j.colsurfb.2025.114596","url":null,"abstract":"<div><div>Lanthanide (Ln) elements form a cofactor complex with pyrroloquinoline quinone (PQQ) in bacterial alcohol dehydrogenases (Ln^3 +-ADH). The lanthanide elements did not support Ln³⁺-ADH activity equally, with only early lanthanides (La³⁺-Gd³⁺) promoting high enzyme activity. However, the early lanthanides did not promote the activity equally and the detailed mechanism of Ln³⁺-ADH exhibiting different activity in the presence of different light Lns remains obscure. To uncover the role of lanthanides in promoting Ln³⁺-ADH activity, we systemically characterized the activity of an Ln³⁺-ADH from <em>Pseudomonas putida</em> KT2440 (PedH) in the presence of various Ln³⁺ ions. In the results, enzyme activity displayed a bell-shaped trend along with the lanthanide series, with Nd³⁺ providing the highest activity. Active site mutation analysis revealed that modifying the number of coordinating ligands shifted the metal preference of the enzyme. DFT calculation revealed that the HOMO-LUMO gap, substrate interaction energy and metal ions binding distances were critical for the lanthanides in promoting enzyme activity. This work shed light on the critical role of metal ions in Ln³⁺-ADH catalysis, providing insights for future exploration and engineering of Ln-dependent proteins.</div></div>","PeriodicalId":279,"journal":{"name":"Colloids and Surfaces B: Biointerfaces","volume":"251 ","pages":"Article 114596"},"PeriodicalIF":5.4,"publicationDate":"2025-02-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143527521","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Biomaterial-associated infections: Their development, characterization, prevention and treatment","authors":"Theerthankar Das ,&nbsp;Reza Nejadnik ,&nbsp;Virginia Vadillo Rodríguez ,&nbsp;Yong Liu ,&nbsp;Guruprakash Subbiahdoss","doi":"10.1016/j.colsurfb.2025.114595","DOIUrl":"10.1016/j.colsurfb.2025.114595","url":null,"abstract":"<div><div>Biomaterials including catheters, artificial joints, dental implants, and contact lenses are highly susceptible to bacterial adhesion, colonization, and biofilm formation, leading to biomaterials-associated infections (BAIs). BAIs pose a significant challenge to the healthcare sector, contributing to increased morbidity, mortality, and economic burden. Preventing bacterial adhesion and biofilm formation is crucial to eliminate BAIs. This special issue, dedicated to Prof. Henk J. Busscher (The University of Groningen, The Netherlands) on the occasion of his retirement, presents original research showcasing innovative strategies to combat biofilm formation on biomaterial surfaces and prevent BAIs. Notable approaches include graphene oxide-copper combinational coatings on cotton fabrics, which exhibited a significant reduction, in <em>Staphylococcus aureus</em> and <em>Pseudomonas aeru</em>ginosa adhesion. Another study demonstrated vancomycin functionalized zwitterionic polymer brush coatings showed antimicrobial and antiadhesive properties against <em>Staphylococcus aureus</em> and <em>Escherichia coli</em> biofilms. Additionally, photokilling-based prevention of bacterial colonization emerges as a promising strategy. Importantly, this special issue also underscores the limitations of mono-culture models in biomaterial testing and highlights the necessity of <em>in vitro</em> co-culture assays integrating bacterial and mammalian cells for a more physiologically relevant evaluation of BAIs.</div></div>","PeriodicalId":279,"journal":{"name":"Colloids and Surfaces B: Biointerfaces","volume":"251 ","pages":"Article 114595"},"PeriodicalIF":5.4,"publicationDate":"2025-02-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143527462","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Designing a T cell multi-epitope vaccine against hRSV with reverse vaccinology: An immunoinformatics approach","authors":"Peibin Guan ,&nbsp;Congyan Qi ,&nbsp;Guojin Xu ,&nbsp;Can Sheng ,&nbsp;Siqi Sun ,&nbsp;Zhicheng Zhou ,&nbsp;Shulei Jia","doi":"10.1016/j.colsurfb.2025.114599","DOIUrl":"10.1016/j.colsurfb.2025.114599","url":null,"abstract":"<div><div>As an infectious viral pathogen, human respiratory syncytial virus (hRSV) can cause severe respiratory infections and is recognized as one of the highest priority pathogens by the World Health Organization (WHO). Although vaccines play an important role in disease prevention and transmission, the wild-type virus is usually prone to immune escape due to the relatively high mutation rate of biological proteins. Therefore, designing a broad-spectrum hRSV vaccine is essential to provide extensive protection against multiple viral variants. Using a consensus sequence approach, we designed a broad-spectrum T-cell epitope vaccine composed of 385 amino acids, consisting of 12 CTLs and 5 HTLs from the fusion protein and glycoprotein. The designed multi-epitope vaccine was expected to have non-allergenicity, high population coverage, strong antigenicity and immunogenicity, appropriate physical and chemical properties, and high solubility. Meanwhile, the structure of the vaccine had a high similarity to that of the natural virus. In addition, through structural biology analysis, the constructed vaccine achieved robust structural compactness and binding stability. Computer-generated immunological simulations indicated that the vaccine could elicit realistic immune responses in humans. The designed vaccine showed good binding affinity and molecular and immune simulation. In conclusion, the broad-spectrum hRSV vaccine could be an excellent candidate for preventing hRSV infection. The employed prediction pipeline was proved to be an efficient method for screening immunogenic epitopes of additional pathogens.</div></div>","PeriodicalId":279,"journal":{"name":"Colloids and Surfaces B: Biointerfaces","volume":"251 ","pages":"Article 114599"},"PeriodicalIF":5.4,"publicationDate":"2025-02-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143527520","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Screening lipid nanoparticles using DNA barcoding and qPCR","authors":"Dawei Liu ,&nbsp;Xing Wang ,&nbsp;Letao Xu ,&nbsp;Zahraa Nima Saeed Al-Delfi ,&nbsp;Zelalem Addis Mekonnen ,&nbsp;Song Gao ,&nbsp;Branka Grubor-Bauk ,&nbsp;Chun-Xia Zhao","doi":"10.1016/j.colsurfb.2025.114598","DOIUrl":"10.1016/j.colsurfb.2025.114598","url":null,"abstract":"<div><div>Quantifying the biodistribution of lipid nanoparticles (LNPs) is critical for optimizing mRNA delivery systems, yet current approaches have inherent limitations. This study introduces a cost-effective method utilizing double-stranded DNA (dsDNA) barcodes and quantitative polymerase chain reaction (qPCR) for rapid analysis of a small library of mRNA-LNPs biodistribution and functional delivery <em>in vivo</em>. Three unique 100-bp dsDNA barcodes were designed to represent for three FDA-approved LNP formulations. Concurrently, these three formulations carrying luciferase mRNA were mixed with DNA-barcoding LNPs as a pool. Following intravenous administration of the pooled LNPs in mice, qPCR analysis revealed the highest abundance of DNA barcodes and accumulation of luciferase mRNA in spleen, with positive correlation between barcodes presence and mRNA localization across organs, validating DNA barcodes as reliable indicators of mRNA-LNPs biodistribution <em>in vivo</em>. Bioluminescence imaging further confirmed successful delivery and protein translation of luciferase mRNA facilitated by the LNPs <em>in vivo</em>. Integrating DNA barcodes for biodistribution analysis and luciferase mRNA for assessing functional delivery enabled comprehensive evaluation of LNP performance. This robust methodology provides valuable insights into the localization patterns and mRNA delivery capabilities of different LNP formulations, paving the way for the development of more effective and targeted mRNA-based therapeutics.</div></div>","PeriodicalId":279,"journal":{"name":"Colloids and Surfaces B: Biointerfaces","volume":"251 ","pages":"Article 114598"},"PeriodicalIF":5.4,"publicationDate":"2025-02-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143521317","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Editorial for Special Issue “Biomaterial-associated infections: Their development, characterization, prevention and treatment”","authors":"Theerthankar Das,&nbsp;Reza Nejadnik,&nbsp;Virginia Vadillo Rodríguez,&nbsp;Yong. Liu,&nbsp;Guruprakash Subbiahdoss","doi":"10.1016/j.colsurfb.2025.114594","DOIUrl":"10.1016/j.colsurfb.2025.114594","url":null,"abstract":"","PeriodicalId":279,"journal":{"name":"Colloids and Surfaces B: Biointerfaces","volume":"251 ","pages":"Article 114594"},"PeriodicalIF":5.4,"publicationDate":"2025-02-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143511342","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Enzyme mimics based on self-assembled peptide functionalized with graphene oxide for polyethylene terephthalate degradation","authors":"Xia Li ,&nbsp;Yaoling Zhou ,&nbsp;Jingchao Yue ,&nbsp;Mengyu Sun ,&nbsp;Xiangmin Lei ,&nbsp;Piwu Li ,&nbsp;Jianpeng Li ,&nbsp;Dengyue Sun ,&nbsp;Zhixiong Zeng","doi":"10.1016/j.colsurfb.2025.114588","DOIUrl":"10.1016/j.colsurfb.2025.114588","url":null,"abstract":"<div><div>The degradation of polyethylene terephthalate (PET) has garnered notable attention owing to its widespread accumulation and the challenges associated with its breakdown. Herein, the enzyme mimics with PET-hydrolytic activity were developed by combining peptide nanofibers with graphene oxide (GO). Inspired by native enzymes, we designed self-assembled peptides that included active amino acids (serine, histidine, aspartate and tryptophan) and different hydrophobic amino acids, with a 9-fluorenylmethoxycarbonyl group at the N-terminus. Our comparison of hydrophobic amino acids revealed that their content not only influenced the higher-order assembly of peptide but also affected molecular conformation and PET degradation ability. By co-assembling two peptides with catalytic and binding sites in a 1:1 ratio, a more effective active enzyme mimic was constructed which was owning to the cooperative interactions among the active amino acids; in addition, hydrogen bonds and π-π stacking interactions were the main forces in enhancing catalytic effects. To further improve PET-hydrolytic ability, the co-assembled enzyme mimic was functionalised with GO through π–π stacking. This GO-peptide nanofiber hybrid exhibited increased PET-hydrolytic, as GO provided a hydrophobic microenvironment for substrate attraction and abundant carbon for facilitating proton transfer. The GO-peptide nanofiber hybrid as enzyme mimics will be a promising material for PET degradation.</div></div>","PeriodicalId":279,"journal":{"name":"Colloids and Surfaces B: Biointerfaces","volume":"251 ","pages":"Article 114588"},"PeriodicalIF":5.4,"publicationDate":"2025-02-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143488430","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Influence of consortium culture and mixed culture on carbon steel corrosion in B30 storage system","authors":"Ardiyan Harimawan,&nbsp;Hary Devianto,&nbsp;Byan Baihaqi,&nbsp;Nilam Khairon Nisa,&nbsp;Christian Aslan","doi":"10.1016/j.colsurfb.2025.114587","DOIUrl":"10.1016/j.colsurfb.2025.114587","url":null,"abstract":"<div><div>B30, which consisted of 30 %-v biodiesel and 70 %-v petrodiesel, is a renewable fuel that is being developed in Indonesia. In the B30 storage system, microbes utilize B30 and cause corrosion of carbon steel. This research aims to compare the interaction effect of consortium culture (<em>S. marcescens</em> – <em>B. megaterium</em> and <em>S. marcescens</em> – <em>B. licheniformis</em>) and mixed culture on the corrosion of carbon steel in the B30 storage system. The experiment was carried out by immersing carbon steel ST-37 specimens in B30 test medium for 21 days. Sample testing and analysis includes the number of microbial colonies, chemical bonds of biofilm composition, morphology of biofilms and metal surfaces, corrosion rate and corrosion products. The results shows that antagonistic interactions occurred in the consortium culture, resulting in the decrease of corrosion rate. Meanwhile, synergistic interaction occurred between the microbes in the mixed culture, resulting in higher corrosion rate. The corrosion mechanism that occurs in consortium culture and mixed culture involves the same electrochemical reactions.</div></div>","PeriodicalId":279,"journal":{"name":"Colloids and Surfaces B: Biointerfaces","volume":"251 ","pages":"Article 114587"},"PeriodicalIF":5.4,"publicationDate":"2025-02-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143521316","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Prolonged local retention of vancomycin achieved by a multivesicular liposomes in thermoresponsive gel system for the prevention and treatment of intervertebral disc infection","authors":"Zhencheng Gao ,&nbsp;Hongxia Fan ,&nbsp;Lutong He ,&nbsp;Yu Zhang ,&nbsp;Tian Yin ,&nbsp;Haibing He ,&nbsp;Xing Tang ,&nbsp;Yanjiao Wang ,&nbsp;Jingxin Gou","doi":"10.1016/j.colsurfb.2025.114593","DOIUrl":"10.1016/j.colsurfb.2025.114593","url":null,"abstract":"<div><div>The therapeutic efficacy of intervertebral disc (IVD) infections treated with intravenous vancomycin (VCM) is often limited by inadequate blood supply to the IVD. In this study, we developed a localized and sustained-release drug delivery system for the intradiscal administration of VCM. First, VCM-loaded multivesicular liposomes (VCM-MVLs) were prepared using a two-step emulsification process, and we investigated the effects of the preparation process and formulation composition on the quality of the MVLs. The prepared MVLs exhibited an encapsulation efficiency of 92.08 ± 6.44 %, a particle size of 30.35 μm, and a sustained release over 12 days. Subsequently, VCM-MVLs were incorporated with PLGA-PEG-PLGA (PPP) to create a thermosensitive hydrogel composite formulation (VCM-MVL-PPP), which demonstrated a gelling temperature of 28.4°C and exhibited prolonged sustained release over 20 days. The antimicrobial activity of VCM-MVL-PPP was maintained for an extended duration of 21 days. Additionally, the VCM-MVL-PPP demonstrated an optimal retention capacity of 14 days in the <em>in vivo</em> retention assay. <em>In vivo</em> safety results confirmed the high biocompatibility and negligible toxicity of the formulation. In conclusion, the presence of PPP as a secondary release barrier for the drug released from MVLs allowed VCM-MVL-PPP to exhibit prolonged drug release and retention in the IVDs compared to VCM-MVLs. Our findings provide a valuable reference for peptide delivery and the treatment of disc infections.</div></div>","PeriodicalId":279,"journal":{"name":"Colloids and Surfaces B: Biointerfaces","volume":"251 ","pages":"Article 114593"},"PeriodicalIF":5.4,"publicationDate":"2025-02-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143488429","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}