Colloids and Surfaces B: Biointerfaces最新文献

{"title":"Functionalized hydroxypropyl-β-cyclodextrin inclusion complex for combined tumor therapy through intelligent delivery of paclitaxel and polarization of M2-like tumor associated macrophages","authors":"Jia Fu ,&nbsp;Wei Zhao ,&nbsp;Na Liang ,&nbsp;Shaoping Sun","doi":"10.1016/j.colsurfb.2025.114654","DOIUrl":"10.1016/j.colsurfb.2025.114654","url":null,"abstract":"<div><div>The treatment outcomes of chemotherapy are far from satisfactory due to suboptimal cellular uptake and inadequate intracellular release of antitumor drugs. Meanwhile, M2-like tumor associated macrophages (TAMs) in the tumor microenvironment promote cancer growth and metastasis. The combined strategy of chemotherapy and reprogramming M2-like TAMs within tumor microenvironment has emerged as a novel paradigm for cancer therapy. Hence, a pH-sensitive double-targeted inclusion complex was constructed for combined cancer therapy through the controlled paclitaxel (PTX) delivery and re-education of M2-like TAMs. The inclusion complex employed arginine and biotin modified hydroxypropyl-β-cyclodextrin (Arg-CD-Bio) as the host, with benzimidazole and mannose modified hyaluronic acid (BM-HA-Man) as the pH-sensitive plug. PTX was encapsulated in the inclusion complex. In vitro experiments indicated that the Arg-CD-Bio/BM-HA-Man inclusion complex could facilitate the specific release of PTX at the tumor site. The inclusion complex could be effectively internalized by M2-like TAMs and MCF-7 cells and further reprogramme M2-like TAMs to M1-like TAMs. In vivo antitumor therapy in 4T1 tumor-bearing mice had achieved remarkable suppression efficacy. These results suggested that the PTX-loaded Arg-CD-Bio/BM-HA-Man inclusion complex was a promising platform for efficient cancer treatment.</div></div>","PeriodicalId":279,"journal":{"name":"Colloids and Surfaces B: Biointerfaces","volume":"252 ","pages":"Article 114654"},"PeriodicalIF":5.4,"publicationDate":"2025-03-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143734953","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Properties and potential uses of a biosurfactant extract obtained from corn steep water by a dialysis process","authors":"A. Martínez-Arcos ,&nbsp;M. Reig ,&nbsp;J.M. Cruz ,&nbsp;J.L. Cortina ,&nbsp;B. Pérez-Cid ,&nbsp;A.B. Moldes ,&nbsp;X. Vecino","doi":"10.1016/j.colsurfb.2025.114649","DOIUrl":"10.1016/j.colsurfb.2025.114649","url":null,"abstract":"<div><div>There is currently a significant demand for more sustainable and compatible ingredients and additives not only for cosmetic and pharmaceutical formulations but also for applications within the agrifood industry. This demand is emphasized by regulations like the COSMOS-standard, which classifies, for instance, cosmetic formulations based on ingredients that meet stringent criteria for their downstream processes promoting physical separation. In this research, a biosurfactant extract derived from a naturally fermented stream within the corn-wet milling industry was obtained for the first time through physical separation methods based on a dialysis process at lab scale. This was done as an alternative to the use of organic solvents. Subsequently, the extract underwent comprehensive characterization and assessment to ascertain its efficacy as a solubilizer, emulsifier, and spreading and wetting agent. The findings revealed that this biosurfactant extract is composed of glycopeptides and peptides, like the biosurfactant extract produced by lactic acid bacteria, but with lower critical micellar concentration (CMC). Moreover, the biosurfactant extract exhibited non-irritating properties (Mucosal Irritation Score &lt; 0.07) and demonstrated interesting capabilities as a booster in the formulation of Pickering emulsions. Furthermore, it demonstrated a spreading capacity comparable to that of sodium dodecyl sulphate (SDS); but not a good solubilization of chicken broth cubes. However, its most noteworthy characteristic was its remarkable wettability capacity, which holds significant promise for applications in the cosmetic industry as a humectant agent, aligning with the standards set in COSMOS standard.</div></div>","PeriodicalId":279,"journal":{"name":"Colloids and Surfaces B: Biointerfaces","volume":"252 ","pages":"Article 114649"},"PeriodicalIF":5.4,"publicationDate":"2025-03-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143716086","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Investigations into the topical hemostatic and wound healing efficacy of binary Hyaluronic acid/Collagen membranes coated with tranexamic acid and vitamin K; InVitro","authors":"Wareef H Alnawwar ,&nbsp;Abeer Abdulaziz H. Bukhari","doi":"10.1016/j.colsurfb.2025.114647","DOIUrl":"10.1016/j.colsurfb.2025.114647","url":null,"abstract":"<div><div>A hemostatic dressing refers to a specific category of medical dressings that have been developed for managing and controlling bleeding. A synthesized hyaluronic acid/collagen (HA/COL) membrane integrated with tranexamic acid (TRAX) and/or vitamin K (Vit K) using the lyophilization technique is fabricated, followed by physical, chemical, and biological assessment. The clotting time and coagulation variables, as prothrombin (PT), partial thromboplastin time (PPT), and international normalized ratio (INR), were also assessed. The antimicrobial properties were tested against Gram-positive &amp; Gram-negative bacteria, yeast, and fungi. The obtained membranes exhibited porous and swellable characteristics, demonstrating their ability to encapsulate vitamin K and/or tranexamic acid effectively. The cell studies demonstrated that the samples containing vitamin K exhibited less toxicity, in contrast to the TRAX compounds. The assessment of cytotoxicity is substantiated by conducting research on wound healing and determining the IC50 values. Additionally, the samples of vitamin K exhibited rapid clotting, which was corroborated by assessing the coagulation blood factors such as PT, PTT, and INR. The antibacterial activity seen in all samples can be attributed to the presence of HA among the membrane constituents. It is advisable to integrate vitamin K into the HA/COL membrane rather than within TRAX or a combination of TRAX.</div></div>","PeriodicalId":279,"journal":{"name":"Colloids and Surfaces B: Biointerfaces","volume":"252 ","pages":"Article 114647"},"PeriodicalIF":5.4,"publicationDate":"2025-03-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143734954","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Electrospun amine-modified polysuccinimide/polycaprolactone nanofiber hydrogel dressings as antibacterial and hemostatic wound dressing applications","authors":"Jialu Pan ,&nbsp;Qi Meng ,&nbsp;Li Zhang ,&nbsp;Caidan Zhang ,&nbsp;Shaohua Wu ,&nbsp;Huiyuan Zhai","doi":"10.1016/j.colsurfb.2025.114648","DOIUrl":"10.1016/j.colsurfb.2025.114648","url":null,"abstract":"<div><div>Design and construction of novel dressings with appropriate structure and multiple bio-functions are urgently required for the wound treatment applications. In this study, a series of wound dressings composed of amine-modified polysuccinimide (PSI)/polycaprolactone (PCL) hydrogel nanofibers were fabricated by using the conjugated electrospinning and the chemical crosslinking post-treatment. All the dressings with different PSI/PCL ratios could effectively maintain the fibrous morphology even after the chemical crosslinking process. The ultimate stress and Young's modulus of the PSI-NH₂/PCL dressings significantly increased with higher PCL content. In particular, when the proportion of PCL reached 40 % (with a PSI/PCL mass ratio of 60/40), the PSI-NH₂/PCL dressing exhibited the most excellent mechanical properties, with a Young's modulus of 31.9 ± 8.9 MPa and an ultimate stress of 7.4 ± 0.4 MPa, which were significantly higher than the other samples. Furthermore, all samples exhibited a high swelling ratio of over 350 %, although a noticeable decrease in swelling properties was observed when the content of PCL increased. In addition, all the PSI-NH₂/PCL dressings showed a high antibacterial activity (&gt; 99 %) against both <em>Escherichia coli</em> (<em>E. coli</em>) and <em>Staphylococcus aureus</em> (<em>S. aureus</em>), which demonstrated that the addition of PCL has no significant influence on the antibacterial activity of the PSI. Moreover, all the PSI-NH₂/PCL nanofiber hydrogel dressings had excellent biocompatibility to human dermal fibroblasts (HDFs). Importantly, all the PSI-NH₂/PCL nanofiber hydrogel dressings presented excellent hemostatic capacity. Notably, even the dressing with the highest PCL content still demonstrated a 71.8 % reduction in bleeding compared to the control group. In summary, our PSI-NH₂/PCL nanofiber hydrogel dressings with high absorption, biocompatibility and extracellular matrix (ECM)-mimicking capacities, as well as great mechanical, antibacterial, and hemostatic properties are excellent as a promising wound dressing.</div></div>","PeriodicalId":279,"journal":{"name":"Colloids and Surfaces B: Biointerfaces","volume":"252 ","pages":"Article 114648"},"PeriodicalIF":5.4,"publicationDate":"2025-03-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143680036","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"All-in-one multifunctional tri-block glycopolymers for targeted delivery of cisplatin and cancer chemotherapy","authors":"Sichen Li ,&nbsp;Difei Zhang ,&nbsp;Yi Li,&nbsp;Jingjing Zhou,&nbsp;Jinghua Chen,&nbsp;Yan Zhang","doi":"10.1016/j.colsurfb.2025.114639","DOIUrl":"10.1016/j.colsurfb.2025.114639","url":null,"abstract":"<div><div>Cisplatin (CDDP) as a first-line chemotherapy drug has long suffered drawbacks of severe side effects and poor pharmacokinetics. Thus, various nano-carriers of complex architectures and multi-step modifications have been developed to overcome these challenges, but a simple delivery system with integrated multi-functions is still in demand. Herein, we synthesized a new type of glyco-based triple hydrophilic block copolymers for the delivery of CDDP and thereof cancer therapy. The incorporated glucuronic acid part is complexed with CDDP with high entrapment efficiency, while providing pH-responsive release in the acidic tumor microenvironment. The galactose segment is implanted for liver-targeting effect, which can also significantly lower the side effects of CDDP. As a result, the CDDP-loaded nanoparticle <strong>PGG2/Pt</strong> showed selectively faster endocytosis rates into HepG2 cells in vitro, with the calculated IC₅₀ value even comparable to that of free CDDP. The in vivo experiments on a HepG2-bearing mouse model, <strong>PGG2/Pt</strong> showed excellent anti-tumor activity and enhanced drug accumulation on tumor, together with much lowered nephrotoxicity, hepatotoxicity, and splenic toxicity. This work provides a new strategy for CDDP delivery with higher loading efficiency as well as biosafety.</div></div>","PeriodicalId":279,"journal":{"name":"Colloids and Surfaces B: Biointerfaces","volume":"252 ","pages":"Article 114639"},"PeriodicalIF":5.4,"publicationDate":"2025-03-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143680034","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Tuning structure and morphology of lipidic cubosomes by encapsulation of novel porphyrin-derivatives","authors":"Valeria D’Annibale ,&nbsp;Leonardo Ariodante ,&nbsp;Claudia Marconi ,&nbsp;Luca Piccirillo ,&nbsp;Peter Jönsson ,&nbsp;Andrea D’Annibale ,&nbsp;Donato Monti ,&nbsp;Anita Scipioni ,&nbsp;Karin Schillén ,&nbsp;Luciano Galantini ,&nbsp;Marco Fornasier","doi":"10.1016/j.colsurfb.2025.114646","DOIUrl":"10.1016/j.colsurfb.2025.114646","url":null,"abstract":"<div><div>Cubosomes are non-lamellar lipid nanoparticles that have drawn a significant attention in the field of nanomedicine due to their tunable properties. However, the formation of vesicles during the preparation of cubosomes, and the presence of mixed bicontinuous cubic phases, may lead to artifacts and lack of correlation between the physico-chemical and biological characterization. In this work, we have formulated cubosomes composed by monoolein as building block and triblock copolymer Pluronic® F108 as a stabilizer, encapsulating three porphyrin derivatives: two attached to bile acid moieties and one to a tetrapeptide to be used for potential theranostic applications. First, the effect of the cargo concentration (0.25, 0.50 and 1.00 mg/mL, for all three molecules) was evaluated on the structure, showing that the bile acid derivatives did not affect the self-assembly of the lipid providing only <em>Pn3m</em> phases; however, a mixed phase <em>Pn3m</em> + <em>Im3m</em> and a subsequent loss in crystallinity were induced by increasing concentrations of the tetrapeptide derivative. Overall, the encapsulation of the three molecules at 25 and 37 ^∘C did not affect neither the hydrodynamic size nor the polydispersity of the cubosomes, influencing mainly the <em>ζ</em>-potential - positive in the case of the tetrapeptide and negative for the bile acid derivatives. The samples formulated with 0.50 mg/mL exhibited higher colloidal stability over time, with no significant changes in size or <em>ζ</em>-potential for over a month. Interestingly, the formulations containing the bile acid derivatives displayed the typical morphology of cubosomes in solution and a reduced number of vesicles (ca. 60:40 as cubosomes-to-vesicles ratio), whereas the sample containing the porphyrin attached to the tetrapeptide led to a ratio of cubosomes-to-vesicles estimated as 26:74, similar to the results of the empty formulation. The experiments at body temperature highlighted that the structure of the different formulations was not affected in a significant manner with retention of the phases observed at room temperature. The promising physico-chemical properties, especially at body temperature, could make these samples suitable as nanoplatforms for drug delivery applications.</div></div>","PeriodicalId":279,"journal":{"name":"Colloids and Surfaces B: Biointerfaces","volume":"252 ","pages":"Article 114646"},"PeriodicalIF":5.4,"publicationDate":"2025-03-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143734952","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A shape memory polynorbornene-based dynamic cell culture platform capable of providing pure mechanical strains","authors":"Weilong Xu ,&nbsp;Hao Li ,&nbsp;Yanbin Lin ,&nbsp;Di Wu ,&nbsp;Xiuyong Liao ,&nbsp;Yanfeng Luo","doi":"10.1016/j.colsurfb.2025.114645","DOIUrl":"10.1016/j.colsurfb.2025.114645","url":null,"abstract":"<div><div>Shape memory polymers (SMPs) can actively generate recovery strain during shape recovery. This characteristic endows SMPs with the potential for dynamic cell culture platforms capable of providing cells with strain stimulation. However, a critical challenge encountered by current SMP-based platforms is that, besides providing recovery strain, shape recovery can alter surface topography and chemistry. Given that both surface topography and chemistry influence cell behavior, decoupling the recovery strain from the alterations in surface topography and chemistry becomes greatly important. In this work, polynorbornene (PNB), an amorphous homopolymer with a shape memory temperature close to the body temperature, was fabricated into films. We found that the shape recovery of the PNB films does not alter the surface chemistry and topography but produces pure recovery strain. The recovery strain is featured with a compressive strain (up to ∼50 %) along and a tensile strain (up to ∼40 %) perpendicular to the stretching direction, which combine to facilitate the orientation of rat mesenchymal stem cells via both passive and active modes related to the F-actin cytoskeletons. These findings confirm that PNB-based films can act as an effective dynamic cell culture platform, which adds a new alternative for smart mechanical loading in biomedical fields.</div></div>","PeriodicalId":279,"journal":{"name":"Colloids and Surfaces B: Biointerfaces","volume":"252 ","pages":"Article 114645"},"PeriodicalIF":5.4,"publicationDate":"2025-03-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143679990","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Achievements in 3D printing of silica-based materials for bone tissue engineering","authors":"Raquel Rodríguez-González ,&nbsp;Luis M. Delgado ,&nbsp;Román A. Pérez","doi":"10.1016/j.colsurfb.2025.114640","DOIUrl":"10.1016/j.colsurfb.2025.114640","url":null,"abstract":"<div><div>Silica-based materials have been commonly studied in the field of bone tissue regeneration, due to their high bioactivity and osteogenic properties. There are two main strategies to obtain silica-based materials, a melt-quenching process using high temperatures, or a sol-gel reaction which can be carried out at mild conditions. Both techniques allow the preparation of calcium silicates and bioactive glasses, but pure silica can only be prepared through the sol-gel method. Furthermore, current clinical treatments require personalized scaffolds and these materials can be combined with the use of 3D printing techniques to obtain patient-specific scaffolds in a fast and precise fabrication process. This review focuses on the different silica-based 3D printable materials available nowadays as well as their physical, chemical and biological properties. Using high temperature, composites can be developed using Fused Deposition Modelling (FDM), while pure silica scaffolds can be prepared through Selective Laser Sintering (SLS) using silica particles. Moreover, silica particles can be 3D printed when combining polymeric binders and SLS, Stereolitography (SLA) or Direct Ink Writing (DIW); however, binder has to be removed at high temperatures after 3D printing. Alternatively, 3D printable silica materials can be obtained at mild temperatures through DIW or SLA, in this case only allowing the printing of composites or hybrids so far, with different proportions of silica. The properties of the resultant materials as well as the main advantages and disadvantages of the printing approaches are summarized in this review, together with the future perspectives in the field of silica 3D printed scaffolds.</div></div>","PeriodicalId":279,"journal":{"name":"Colloids and Surfaces B: Biointerfaces","volume":"252 ","pages":"Article 114640"},"PeriodicalIF":5.4,"publicationDate":"2025-03-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143704482","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Role of chemical groups in regulating membrane tension of mBMSCs under stretch stimulation","authors":"Yunqing Ye ,&nbsp;Haoyang Huang ,&nbsp;Hong Li ,&nbsp;Gang Wu","doi":"10.1016/j.colsurfb.2025.114644","DOIUrl":"10.1016/j.colsurfb.2025.114644","url":null,"abstract":"<div><div>As a crucial mechanobiological regulator, the tension of the cell membrane plays a vital role in governing cellular adhesion, proliferation, and differentiation processes. Additionally, it displayed a dynamic response to mechanical microenvironmental changes. This research systematically examines the mechanoresponsive behaviors of mouse bone marrow mesenchymal stem cells (mBMSCs) that are cultured on poly(dimethylsiloxane) (PDMS) substrates which are functionalized with methyl (-CH₃), amino (-NH₂), and carboxyl (-COOH) groups. Under both static and stretching conditions, it is found that compared with the -CH₃ surface, static culture on the -NH₂ and -COOH functionalized surfaces significantly promotes the proliferation of mBMSCs and upregulates the expression of extracellular matrix adhesion-related genes, especially focal adhesion kinase (FAK) and integrin β1. Morphometric analysis reveals that there are concomitant increases in the cell spreading area and the number of pseudopods on these modified surfaces. Mechanical stretching stimulation not only amplifies these cellular responses but also leads to more uniform FAK distribution. The assessment by atomic force microscopy (AFM) shows that both chemical functionalization (-NH₂/-COOH) and stretch stimulation reduce the deformability of the cell membrane, and the -NH₂ modification exhibits a greater membrane-stiffening effect than -COOH.</div></div>","PeriodicalId":279,"journal":{"name":"Colloids and Surfaces B: Biointerfaces","volume":"252 ","pages":"Article 114644"},"PeriodicalIF":5.4,"publicationDate":"2025-03-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143680037","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Three-dimensional bioactive collagen scaffolds incorporated with titanate nanotubes for tissue regeneration","authors":"Petra Reinehr ,&nbsp;Leonardo Francisco Diel ,&nbsp;Fernando Mendonça Diz ,&nbsp;Gabriela de Souza Balbinot ,&nbsp;Wesley Formentin Monteiro ,&nbsp;Rosane Angélica Ligabue ,&nbsp;Marcelo Lazzaron Lamers ,&nbsp;Marcel Ferreira Kunrath","doi":"10.1016/j.colsurfb.2025.114638","DOIUrl":"10.1016/j.colsurfb.2025.114638","url":null,"abstract":"<div><div>The development of three-dimensional (3D) biomimetic scaffolds for bone and soft tissue engineering has received increasing attention due to their ability to mimic the extracellular matrix (ECM) environment. This study presents the development and characterization of a 3D collagen matrix incorporating titanate nanotubes (TNT) aiming to improve cell migration and biocompatibility, with potential applications in bioink for bone and soft tissue regeneration. TNT were hydrothermally synthesized, and their properties were characterized using materials analysis techniques. After the incorporation of human fibroblasts into the collagen matrices with or without TNT, biological characterization was performed. The results showed that the incorporation of TNT significantly improved the migration efficiency and directionality compared to collagen-only matrices, which were more evident after long-term incubation. This indicates that the addition of TNT to the collagen matrixes improves the mechanical properties, promotes biocompatibility, and induces a superior environment for cell migration. These findings contribute to the development of new biomaterials for tissue engineering and demonstrate the potential of TNT as a key component of bioengineered biomaterials for bone and soft tissue regeneration.</div></div>","PeriodicalId":279,"journal":{"name":"Colloids and Surfaces B: Biointerfaces","volume":"252 ","pages":"Article 114638"},"PeriodicalIF":5.4,"publicationDate":"2025-03-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143716087","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}