Colloids and Surfaces B: Biointerfaces最新文献

{"title":"Poly-tannic acid coated PLGA nanoparticle decorated with antimicrobial peptide for synergistic bacteria treatment and infectious wound healing promotion","authors":"","doi":"10.1016/j.colsurfb.2024.114217","DOIUrl":"10.1016/j.colsurfb.2024.114217","url":null,"abstract":"<div><p>Bacterial infections pose a great threat to human health. Therefore, the development of new antibacterial agents or methods is in urgent need. In this study, we prepared polytannic acid (pTA)-coated PLGA nanoparticles decorated with Dermaseptin-PP (Der), an antimicrobial peptide (AMP), on the surface to obtain PLGA-pTA-Der. This nanoplatform could combine AMPs with photothermal treatment (PTT) mediated by pTA to achieve synergistic bacterial killing. The results of <em>in vitro</em> experiments showed that the PLGA-pTA-Der nanoparticles could eliminate nearly 99 % of <em>Escherichia coli</em> (<em>E. coli</em>) and <em>Staphylococcus aureus</em> (<em>S. aureus</em>) upon near-infrared (NIR) laser irradiation (2.0 W·cm⁻², 5 min), demonstrating excellent antibacterial properties. In addition, the results of atomic force microscopy (AFM) revealed that PLGA-pTA-Der with laser irradiation can greatly destroy the mechanical integrity of the bacterial outer membrane. And the presence of Der could exacerbate the heat damage caused by the PLGA-pTA NPs to the bacteria, which is helpful to reduce the critical temperature required for bacteria killing by PTT. <em>In vivo</em> experiments showed that PLGA-pTA-Der nanoparticles with laser irradiation significantly accelerated the wound healing process and inhibited the growth of bacterial. Moreover, it can achieve a strong photothermal antibacterial effect at a mild temperature (&lt;45℃) and does not cause any obvious thermal damage to the surrounding normal skin tissues. Results of immunofluorescence staining showed that the expression of CD31 (a marker of new blood vessel formation) was significantly higher in the PLGA-pTA-Der + laser group than other groups, while the pro-inflammatory molecule TNF-α was significantly lower, indicating that PLGA-pTA-Der nanoparticles accelerated wound healing by enhancing angiogenesis and reducing the inflammatory response. In conclusion, PLGA-pTA-Der nanoparticles was a promising antimicrobial nanoplatform for treating bacterial infections and promoting wound healing.</p></div>","PeriodicalId":279,"journal":{"name":"Colloids and Surfaces B: Biointerfaces","volume":null,"pages":null},"PeriodicalIF":5.4,"publicationDate":"2024-09-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142158054","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Intrinsic physiochemical insights to green synthesized Ag-decorated GO nanosheet for photoluminescence and in vivo cellular biocompatibility with embryonic zebrafish","authors":"","doi":"10.1016/j.colsurfb.2024.114212","DOIUrl":"10.1016/j.colsurfb.2024.114212","url":null,"abstract":"<div><p>The advancement of nanotechnology and their application has intrigued a significant interest in green synthesis and application of organic and inorganic nanomaterials like graphene oxide (GO) and silver nanoparticles (AgNP). This study explores the intrinsic physiochemical properties of silver (Ag)-decorated graphene oxide (GO) nanosheets synthesized via a green approach, focusing on their photoluminescence behaviour and <em>in vivo</em> cellular biocompatibility with embryonic zebrafish. The nanocomposites were characterized using various spectroscopic and microscopic techniques to elucidate their structural and optical properties. Results reveal that the Ag-decorated GO nanosheets exhibit enhanced photoluminescence compared to pristine GO with an SPR at 405 nm and emission at 676 nm, attributed to the synergistic effects of Ag nanoparticles and GO. In addition, <em>in vivo</em> biocompatibility assessments using embryonic zebrafish demonstrate minimal cytotoxicity and high cellular viability upon exposure to the nanocomposites with an LC50 of 38 µg/ml, indicating their potential for biomedical applications. Further investigations into the interactions between the nanomaterials and biological systems provide valuable insights into their safety profile and suggest their suitability for various biomedical and therapeutic applications. Overall, this study offers a comprehensive understanding of the physiochemical characteristics and biological compatibility of Ag-decorated GO nanosheets, contributing to the advancement of nanotechnology in biomedicine and related fields.</p></div>","PeriodicalId":279,"journal":{"name":"Colloids and Surfaces B: Biointerfaces","volume":null,"pages":null},"PeriodicalIF":5.4,"publicationDate":"2024-09-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142229311","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effect of micropillar density on morphology and migration of low and high metastatic potential breast cancer cells","authors":"","doi":"10.1016/j.colsurfb.2024.114214","DOIUrl":"10.1016/j.colsurfb.2024.114214","url":null,"abstract":"<div><p>Study of cell migration in cancer is crucial to the comprehension of the processes and factors that govern tumor spread. Cancer cells migrate invading tissues, causing alterations in cell adhesion, cytoskeleton, and signaling pathways. Little is known about the physical attributes of cancer cells that change when interacting with microenvironments. In this work, the local topography of the ECM has been mimicked through micropillar array substrates. MDA-MB-231 and MCF-7 breast cancer cells, exhibiting high and low metastatic potential, respectively, were analyzed. Differences in morphology and migration of the cells were investigated by examining the cell spreading area, circularity, aspect ratio, migration speed, and migration path. This work encountered that none of the studied cell lines have preferential orientation migrating on uniform patterns. In contrast, cell migration on graded patterns shows preferential orientation along the longitudinal direction from sparser to denser zones which is significantly influenced by substrate stiffness and indicates that both cell lines can sense the spacing gradient and respond to this topographical cue. The migration speed of the breast cancer cell lines significantly decreases from the sparse to medium to dense zones, registering higher values for the MDA-MB-231.</p></div>","PeriodicalId":279,"journal":{"name":"Colloids and Surfaces B: Biointerfaces","volume":null,"pages":null},"PeriodicalIF":5.4,"publicationDate":"2024-09-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142164180","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Atrazine concentration detection based on NiAl-layer double hydroxides nanosheets synaptic transistor","authors":"","doi":"10.1016/j.colsurfb.2024.114210","DOIUrl":"10.1016/j.colsurfb.2024.114210","url":null,"abstract":"<div><p>A transistor inspired by biological systems, which possesses synaptic and sensing capabilities, has demonstrated significant promise in the field of neuromorphic electronics and sensory systems resembling the human brain. Despite the remarkable advancements in emulating neuromorphic operations, the development of a synaptic FET with a bionic architecture, extended lifespan, minimal energy usage, and marker monitoring capability remains challenging. In this work, a synaptic transistor based on NiAl-layer double hydroxides nanosheets is reported. The synaptic transistor exhibits a significant ratio of on/off current (1.35×10⁷) and possesses a high transconductance value (10.05 mS). The successful emulation included key synaptic characteristics, such as excitatory/inhibitory postsynaptic current, paired-pulse facilitation/depression, short-term plasticity spike amplitude-dependent plasticity, spike timing-dependent plasticity, as well as spike number-dependent plasticity. A consumption of 64.8 pJ per spike was achieved as a result of the efficient carrier transfer pathway facilitated by the nanosheets composed of double hydroxides. In addition, the FET's linear detection region (with a coefficient <em>R</em>²=0.811) encompassed atrazine concentrations ranging from 10 pg/mL to 0.1 μg/mL, thanks to its high surface area and significant transconductance. Therefore, this study presents a potential approach for achieving energy-efficient neuromorphic computing and high-performance synaptic devices.</p></div>","PeriodicalId":279,"journal":{"name":"Colloids and Surfaces B: Biointerfaces","volume":null,"pages":null},"PeriodicalIF":5.4,"publicationDate":"2024-09-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142144521","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Enzymeless detection and real-time analysis of intracellular hydrogen peroxide released from cancer cells using gold nanoparticles embedded bimetallic metal organic framework","authors":"","doi":"10.1016/j.colsurfb.2024.114209","DOIUrl":"10.1016/j.colsurfb.2024.114209","url":null,"abstract":"<div><p>Abnormal cell growth and proliferation can lead to tumor formation and cancer, one of the most fatal diseases worldwide. Hydrogen peroxide (H₂O₂) has emerged as a cancer biomarker, with its concentration being crucial for distinguishing cancer cells from normal cells. Herein, a cost-effective and enzymeless electrochemical sensing system for the monitoring of intracellular H₂O₂ has been constructed. The sensor is fabricated using gold nanoparticles embedded bimetallic copper/nickel metal organic framework (Au-CNMOF) immobilized reduced graphene oxide (RGO) modified screen printed electrode (SPE). The synthesized materials were characterized and confirmed by XRD, FTIR, SEM with EDS, and electrochemical analysis. The fabricated sensor displayed a redox peak at a formal potential (<em>E</em>°) of −0.155 V, corresponding to Cu^II/I redox couple of CNMOF in 0.1 M phosphate buffer. Electrochemical investigations revealed that the proposed sensor has a large electrochemical active surface area (1.113 cm²) and a higher surface roughness (5.67). Additionally, the sensor demonstrated excellent electrocatalytic activity towards H₂O₂ at −0.3 V, over a wide linear detection range from 28.5 µM to 4.564 mM with a limit of detection of 4.2 µM (S/N=3). Furthermore, the proposed sensor exhibits excellent stability, repeatability, reproducibility, and good anti-interference activity. Ultimately, the sensor was validated through real-time analysis of H₂O₂ released from cancer cells, successfully quantifying the released H₂O₂. The developed sensor holds great promise for real-time H₂O₂ analysis, with potential applications in clinical diagnostics, biological research and environmental monitoring.</p></div>","PeriodicalId":279,"journal":{"name":"Colloids and Surfaces B: Biointerfaces","volume":null,"pages":null},"PeriodicalIF":5.4,"publicationDate":"2024-09-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142164184","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Biomimetic hydrogel coatings for improving the corrosion resistance, hemocompatibility, and endothelial cell growth of the magnesium alloy","authors":"","doi":"10.1016/j.colsurfb.2024.114204","DOIUrl":"10.1016/j.colsurfb.2024.114204","url":null,"abstract":"<div><p>The fast biodegradation and poor biocompatibility of Mg alloys in physiological environments are still the main problems restricting their application in cardiovascular stents. In this study, the hydrogel coatings (SBMA-AAM) with different proportions of methacryloyl ethyl sulfobetaine (SBMA) and acrylamide (AAM) were built on the surface of AZ31B magnesium alloy through ultraviolet (UV) polymerization. The corrosion degradation behavior, hemocompatibility, and endothelial cell (EC) growth performance of the samples were studied in detail. The findings revealed that the uniform and dense SBMA-AAM coatings could significantly enhance the corrosion resistance. In addition, the hydrogel coatings showed excellent hydrophilicity, which increased the albumin adsorption while inhibiting the fibrinogen adsorption, and thus reduced the platelet adhesion and activation and hemolysis rate, accordingly significantly enhancing their anticoagulant performance. Furthermore, SBMA-AAM hydrogel coating promoted the EC adhesion and proliferation and the vascular endothelial growth factor (VEGF) and nitric oxide (NO) secretion of ECs, which is conducive to promoting endothelialization. When the concentration ratio of SBMA and AAM was 1: 2, the modified magnesium alloy showed the best corrosion resistance and biocompatibility. Therefore, the SBMA-AAM hydrogel coating could effectively regulate the corrosion degradation performance and biocompatibility of Mg alloys, laying a foundation for the application of Mg alloys in cardiovascular stents.</p></div>","PeriodicalId":279,"journal":{"name":"Colloids and Surfaces B: Biointerfaces","volume":null,"pages":null},"PeriodicalIF":5.4,"publicationDate":"2024-09-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142137337","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Biocompatibility and corrosion resistance of drug coatings with different polymers for magnesium alloy cardiovascular stents","authors":"","doi":"10.1016/j.colsurfb.2024.114202","DOIUrl":"10.1016/j.colsurfb.2024.114202","url":null,"abstract":"<div><p>Recently, advances in enhancing corrosion properties through various techniques, and the clinical application of biodegradable cardiovascular stents made from magnesium (Mg) alloys face challenges to corrosion resistance, blood compatibility, and biocompatibility. Drug-eluting stents (DES) offer a solution to enhance the corrosion resistance of Mg alloys while simultaneously reducing the occurrence of restenosis. In this study, WE43 Mg alloy was pretreated using electropolishing technology, and different polymers (PEG and PLLA) were used as drug-polymer coatings for the Mg alloy. At the same time, PTX, an anticoagulant, was incorporated to achieve drug coating of different polymers on WE43 Mg alloy. The corrosion resistance of different polymer-drug coatings was assessed using a plasma solution. Furthermore, in vitro and in vivo tests were used to evaluate the blood biocompatibility of these coatings. The results indicated the PTX-PEG-coated WE43 Mg alloy exhibited the highest corrosion resistance and the most stable drug release profile among the tested coatings. Its hemolysis rate of 0.6 % was within the clinical requirements (&lt;5 %). The incorporation of PEG prevents non-specific protein adsorption and nanoparticle aggregation, enhancing the surface hemocompatibility of WE43 Mg alloy. Therefore, the PTX-PEG coating shows promising potential for application in the development of drug-coated Mg alloy.</p></div>","PeriodicalId":279,"journal":{"name":"Colloids and Surfaces B: Biointerfaces","volume":null,"pages":null},"PeriodicalIF":5.4,"publicationDate":"2024-09-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142164183","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Glutathione-responsive biodegradable nanohybrid for cancer photoacoustic imaging and gas-assisted photothermal therapy","authors":"","doi":"10.1016/j.colsurfb.2024.114205","DOIUrl":"10.1016/j.colsurfb.2024.114205","url":null,"abstract":"<div><p>Photothermal therapy (PTT), particularly in the near-infrared-II (NIR-II) range, has attracted widespread attention over the past years. However, the accompanied inflammatory responses can result in undesirable side effects and contribute to treatment ineffectiveness. Herein, we introduced a novel biodegradable nanoplatform (CuS/HMON-PEG) capable of PTT and hydrogen sulfide (H₂S) generation, aimed at modulating inflammation for improved cancer treatment outcomes. The embedded ultrasmall copper sulphide (CuS) nanodots (1–2 nm) possessed favorable photoacoustic imaging (PAI) and NIR-II photothermal capabilities, rendering CuS/HMON-PEG an ideal phototheranostic agent. Upon internalization by 4T1 cancer cells, the hollow mesoporous organosilica nanoparticle (HMON) component could react with the overproduced glutathione (GSH) to produce H₂S. In addition to the anticipated photothermal tumor ablation and H₂S-induced mitochondrial dysfunction, the anti-inflammatory regulation was also been demonstrated by the downregulation of tumor necrosis factor-alpha (TNF-α), interleukin-6 (IL-6), and interleukin-1beta (IL-1β). More importantly, the modulation of inflammation also promoted wound healing mediated by PTT. This work not only presents a H₂S-based nanomodulator to boost NIR-II PTT but also provides insights into the construction of novel organic/inorganic hybrid nanosystems.</p></div>","PeriodicalId":279,"journal":{"name":"Colloids and Surfaces B: Biointerfaces","volume":null,"pages":null},"PeriodicalIF":5.4,"publicationDate":"2024-09-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142144525","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Injectable dual drug-loaded thermosensitive liposome-hydrogel composite scaffold for vascularised and innervated bone regeneration","authors":"","doi":"10.1016/j.colsurfb.2024.114203","DOIUrl":"10.1016/j.colsurfb.2024.114203","url":null,"abstract":"<div><p>Adequate blood supply and thorough innervation are essential to the survival of tissue-engineered bones. Though great progress has been created in the application of bone tissue engineering technology to bone defect repair, many challenges remain, such as insufficient vascularisation and deficient innervation in newly regenerated bone. In the present study, we addressed these challenges by manipulating the bone regeneration microenvironment in terms of vascularisation and innervation. We used a novel injectable thermosensitive liposome-hydrogel composite scaffold as a sustained-release carrier for basic fibroblast growth factor (bFGF, which promotes angiogenesis and neurogenic differentiation) and dexamethasone (Dex, which promotes osteogenic differentiation). In vitro biological assessment demonstrated that the composite scaffold had sufficient cell compatibility; it enhanced the capacity for angiogenesis in human umbilical vein endothelial cells, and the capacity for neurogenic/osteogenic differentiation in human bone marrow mesenchymal stem cells. Moreover, the introduction of bFGF/Dex liposome-hydrogel composite scaffold to bone defect sites significantly improved vascularisation and innervated bone regeneration properties in a rabbit cranial defect model. Based on our findings, the regeneration of sufficiently vascularised and innervated bone tissue through a sustained-release scaffold with excellent injectability and body temperature sensitivity represents a promising tactic towards bone defect repair.</p></div>","PeriodicalId":279,"journal":{"name":"Colloids and Surfaces B: Biointerfaces","volume":null,"pages":null},"PeriodicalIF":5.4,"publicationDate":"2024-09-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142144527","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Copper tannate nanosheets-embedded multifunctional coating for antifouling and photothermal bactericidal applications","authors":"","doi":"10.1016/j.colsurfb.2024.114208","DOIUrl":"10.1016/j.colsurfb.2024.114208","url":null,"abstract":"<div><p>Implant-associated infections (IAIs), triggered by pathogenic bacteria, are a leading cause of implant failure. The design of functionalized coatings on biomedical materials is crucial to address IAIs. Herein, a multifunctional coating with good antifouling effect and antibacterial photothermal therapy (aPTT) performance was developed. The copper tannate nanosheets (CuTA NSs) were formed <em>via</em> coordination bonding of Cu²⁺ ions and tannic acid (TA). The CuTA NSs were then integrated into the TA and poly(ethylene glycol) (PEG) network to form the TCP coating for deposition on the titanium (Ti) substrates <em>via</em> surface adhesion of TA and gravitational effect. The resulting Ti-TCP substrate exhibited good antifouling property, reactive oxygen species (ROS) scavenging capability and cytocompatibility. The TCP coating exhibited antifouling efficacy in conjunction with aPTT, curtailing the surface adhesion and biofilm formation of pathogens, such as <em>Staphylococcus aureus</em> and <em>Escherichia coli</em>. Notably, the Ti-TCP substrate also exhibited the ability to prevent bacterial infection <em>in vivo</em> in a subcutaneous implantation model. The present work demonstrated a promising approach in designing high-performance antifouling and photothermal bactericidal coatings to combat IAIs.</p></div>","PeriodicalId":279,"journal":{"name":"Colloids and Surfaces B: Biointerfaces","volume":null,"pages":null},"PeriodicalIF":5.4,"publicationDate":"2024-09-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142164185","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}