Colloids and Surfaces B: Biointerfaces最新文献_第2页

AI-driven optimization of spinal implant design using parametric modelling 使用参数化建模的人工智能驱动的脊柱植入物设计优化

IF 5.4 2区医学

Colloids and Surfaces B: Biointerfaces Pub Date : 2025-05-13 DOI: 10.1016/j.colsurfb.2025.114753

Idowu O. Malachi , Adebukola O. Olawumi , B.I. Oladapo

{"title":"AI-driven optimization of spinal implant design using parametric modelling","authors":"Idowu O. Malachi , Adebukola O. Olawumi , B.I. Oladapo","doi":"10.1016/j.colsurfb.2025.114753","DOIUrl":"10.1016/j.colsurfb.2025.114753","url":null,"abstract":"<div><div>This study aimed to enhance vertebral implant design by using a parametric spine model and advanced simulation techniques to evaluate biomechanical behaviours under dynamic physiological conditions using Finite Element Analysis (FEA) in ANSYS Workbench. The primary objective was to refine implant designs to improve surgical outcomes and patient safety. We incorporated the anisotropic material properties of Magnesium-Rare Earth-Zirconium (Mg-RE-Zr) alloys, focusing on their Young's modulus (40–50 GPa), Poisson's ratio (0.35), and yield strengths (0.193 GPa tensile, 0.255 GPa compressive) to simulate real-world stress and deformation scenarios. Using Finite Element Analysis (FEA), we conducted a series of simulations to examine stress distribution and deformation patterns across various implant models under static and dynamic loads. These simulations provided detailed insights, revealing that maximum equivalent stresses could reach up to 0.160 GPa, with deformations ranging from 0.01875 mm at a lower modulus to 0.015 mm at a higher modulus, showcasing the influence of material stiffness on implant performance. The model demonstrated high accuracy, with an error margin of less than 5 % when validated against analysis test data. This research makes a significant contribution to the field by providing a validated method for predicting and enhancing the biomechanical performance of spinal implants, thereby ensuring their reliability and efficacy in clinical applications.</div></div>","PeriodicalId":279,"journal":{"name":"Colloids and Surfaces B: Biointerfaces","volume":"253 ","pages":"Article 114753"},"PeriodicalIF":5.4,"publicationDate":"2025-05-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144068344","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Dual-atom nanozymes: Synthesis, characterization, catalytic mechanism and biomedical applications 双原子纳米酶：合成、表征、催化机理及生物医学应用

IF 5.4 2区医学

Colloids and Surfaces B: Biointerfaces Pub Date : 2025-05-12 DOI: 10.1016/j.colsurfb.2025.114774

Ran Bi , Jingyi Liu , Yuyao Cai , Shuangning Zhang , Maonan Lu , Chenxi Du , Mengyuan Liu , Xinyu Ding , Ke Xiao , Si Li , Tingting Jiang , Shidong Xiang

{"title":"Dual-atom nanozymes: Synthesis, characterization, catalytic mechanism and biomedical applications","authors":"Ran Bi , Jingyi Liu , Yuyao Cai , Shuangning Zhang , Maonan Lu , Chenxi Du , Mengyuan Liu , Xinyu Ding , Ke Xiao , Si Li , Tingting Jiang , Shidong Xiang","doi":"10.1016/j.colsurfb.2025.114774","DOIUrl":"10.1016/j.colsurfb.2025.114774","url":null,"abstract":"<div><div>Dual-atom nanozymes (DAzymes), a novel class of nanozymes featuring dual-metal atomic active centers, mimic the multi-metal synergistic mechanisms of natural enzymes to achieve superior catalytic activity compared to conventional single-atom nanozymes. Their unique dual-atom architecture not only effectively mitigates metal atom aggregation but also significantly enhances substrate adsorption capacity and catalytic efficiency through interatomic electronic coupling and spatial synergy. This structural innovation addresses critical limitations of single-atom nanozymes, including low metal loading and homogeneous active sites. This review systematically summarizes recent advancements in DAzymes: First, we elucidate their design principles and structural advantages, with a focus on precise synthesis strategies (e.g., spatial confinement, coordination stabilization) and atomic-level characterization techniques (e.g., synchrotron radiation-based X-ray absorption spectroscopy, spherical aberration-corrected electron microscopy). By unraveling structure-activity relationships, we clarify the multi-dimensional regulatory mechanisms of dual-atom systems—including coordination environments, electronic coupling, and spatial configurations—on redox enzyme-like activities such as peroxidase and superoxide dismutase mimics. Furthermore, we elaborate on their groundbreaking biomedical applications, including antibacterial and antitumor therapies via reactive oxygen species (ROS) regulation, antioxidant damage repair, and biosensing. This review aims to provide theoretical guidance for the rational design of high-performance DAzymes and to advance their translational applications in precision medicine and intelligent biomaterials.</div></div>","PeriodicalId":279,"journal":{"name":"Colloids and Surfaces B: Biointerfaces","volume":"253 ","pages":"Article 114774"},"PeriodicalIF":5.4,"publicationDate":"2025-05-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143948762","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Mg-doped bioactive glass and tea polyphenols incorporated Janus wound dressing for antibacterial and promoting tissue repair 含镁生物活性玻璃和茶多酚的Janus伤口敷料具有抗菌和促进组织修复的作用

IF 5.4 2区医学

Colloids and Surfaces B: Biointerfaces Pub Date : 2025-05-12 DOI: 10.1016/j.colsurfb.2025.114796

Xin Zhao , Jingjing Jiao , Man Ao , Lin Huang , Peng Xu , Yan Shi , Jiao-jing Shao , Yuanlong Guo , Haibo Xie , Long Li , Qiuyue Ding , Zhu Chen

{"title":"Mg-doped bioactive glass and tea polyphenols incorporated Janus wound dressing for antibacterial and promoting tissue repair","authors":"Xin Zhao , Jingjing Jiao , Man Ao , Lin Huang , Peng Xu , Yan Shi , Jiao-jing Shao , Yuanlong Guo , Haibo Xie , Long Li , Qiuyue Ding , Zhu Chen","doi":"10.1016/j.colsurfb.2025.114796","DOIUrl":"10.1016/j.colsurfb.2025.114796","url":null,"abstract":"<div><div>Bacterial infection, excessive inflammation, and insufficient tissue remodeling capacity are major factors to delayed or impaired wound healing. This study synthesized Mg-doped bioactive glass (MBG) using the sol-gel method. Subsequently, based on polycaprolactone (PCL), MBG and tea polyphenols (TP), a Janus wound dressing with an enhanced hydrophilic and antibacterial outer layer of PCL-TP (PT) and a hydrophobicity-preserved and tissue generation-promoting inner layer of PCL-MBG (PB) was further fabricated using a sequential electrospinning method. This design endowed the dressing with directional fluid transport property to effectively prevent tissue adhesion and exudate infiltration. In vitro drug release results demonstrated that ions such as Si, Mg, Ca, and P were sustainably released up to 5 days at least, while TP could be slowly released for over 7 days. The fibrous membrane loaded with TP showed significant inhibition against S. aureus and E. coli, with increasing antibacterial activity as the increase of TP content. The cytological evaluation confirmed that dressings incorporating with MBG and TP did not exhibit cytotoxicity but significantly promoted cell proliferation. The PB/PT Janus dressing demonstrated an apparent repair area exceeding 99 % in animal experiments, along with a significantly higher number of new hair follicles and collagen ratio compared to the blank and PCL groups. These results indicate the potential of this Janus wound dressing in effectively promoting wound healing.</div></div>","PeriodicalId":279,"journal":{"name":"Colloids and Surfaces B: Biointerfaces","volume":"253 ","pages":"Article 114796"},"PeriodicalIF":5.4,"publicationDate":"2025-05-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143948703","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Merging fusogenic DOPE and a tumour targeted self-assembling biopolymer: Smart hybrid liposomes for drug vectorization in cancer therapy 融合融合融合的融合融合融合的自组装肿瘤靶向生物聚合物：智能杂化脂质体的药物载体在癌症治疗

IF 5.4 2区医学

Colloids and Surfaces B: Biointerfaces Pub Date : 2025-05-12 DOI: 10.1016/j.colsurfb.2025.114786

Ludovica Scorzafave , Eugenia Nicol Manti , Marco Fiorillo, Manuela Curcio, Giuseppe Cirillo, Luca Frattaruolo, Fiore Pasquale Nicoletta, Anna Rita Cappello, Francesca Iemma

{"title":"Merging fusogenic DOPE and a tumour targeted self-assembling biopolymer: Smart hybrid liposomes for drug vectorization in cancer therapy","authors":"Ludovica Scorzafave , Eugenia Nicol Manti , Marco Fiorillo, Manuela Curcio, Giuseppe Cirillo, Luca Frattaruolo, Fiore Pasquale Nicoletta, Anna Rita Cappello, Francesca Iemma","doi":"10.1016/j.colsurfb.2025.114786","DOIUrl":"10.1016/j.colsurfb.2025.114786","url":null,"abstract":"<div><div>In this study, a smart hybrid liposome system was achieved combining a self-assembling biopolymer conjugate (human serum albumin-hyaluronic acid) with targeting and redox-responsive activity with dioleoyl phosphatidylethanolamine, a fusogenic phospholipid. The obtained hybrid liposomal structures were found to possess suitable physicochemical properties for cancer therapy application, with mean size of 65 nm, negative surface charge (-27 mV) and the ability to efficiently encapsulate Doxorubicin in the inner liposomal core with high efficiency. The drug loaded hybrid liposomes (DOX@HBLs) were able to trigger the drug release under simulated acidic and redox conditions of the tumor environment, whereas the biological characterization demonstrated the safety and the selectivity of the formulation, able to target the cancer cells (toxicity similar to that of the free drug) while sparing the healthy cells (viability > 90 % in all cases). Furthermore, compared to free drug, DOX@HBLs were able to reduce cell motility, impair metabolic pathways essential for cancer progression, and effectively inhibit spheroid formation (by almost 50 % after 20 days incubation) in both Estrogen Receptor-positive and Triple Negative Breast Cancer cells, demonstrating their high potential as unconventional drug delivery vectors in cancer therapy.</div></div>","PeriodicalId":279,"journal":{"name":"Colloids and Surfaces B: Biointerfaces","volume":"253 ","pages":"Article 114786"},"PeriodicalIF":5.4,"publicationDate":"2025-05-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143948726","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Green synthesis of nanoceria using Terminalia Arjuna extract for enhanced stability, antioxidant, and anticancer properties than their chemical counterparts 利用阿朱那终叶提取物合成的绿色纳米粒，其稳定性、抗氧化性和抗癌性都比化学制剂强

IF 5.4 2区医学

Colloids and Surfaces B: Biointerfaces Pub Date : 2025-05-12 DOI: 10.1016/j.colsurfb.2025.114798

Purnimajayasree Ramesh , Arunkumar Palaniappan

{"title":"Green synthesis of nanoceria using Terminalia Arjuna extract for enhanced stability, antioxidant, and anticancer properties than their chemical counterparts","authors":"Purnimajayasree Ramesh , Arunkumar Palaniappan","doi":"10.1016/j.colsurfb.2025.114798","DOIUrl":"10.1016/j.colsurfb.2025.114798","url":null,"abstract":"<div><div>Nanoceria, a potent nanozyme, widely explored for biomedical applications, often faces toxicity and stability issues when synthesized chemically. In this study, nanoceria (NC-G) is synthesized via a simple green method using <em>Terminalia arjuna</em> extract as a reducing and capping agent and is compared with chemically synthesized nanoceria (NC-C) for stability, antioxidant, and anti-cancer properties. The mean sizes and surface charge of NC-C and NC-G was found to be 37.78 ± 15.5 nm (-16.2 ± 7.6 mV) and 21.8 ± 5.3 nm (-51.4 ± 8.9 mV) respectively. The percentage of Ce <sup>3 +</sup> and Ce <sup>4+</sup> was determined using XPS analyses. Superoxide dismutase (SOD), catalase and antioxidant regenerative properties of NC-G was determined to have better performance than NC-C. Thus, NC-G demonstrated an improvement in cyto-compatibility when compared to NC-C using MTT assay. Moreover, NC-G showed enhanced intracellular antioxidant and cyto-protective properties under oxidative stress in rat cardiomyocytes cell line (H9C2). Further, both NC-C and NC-G showed dose-dependent anti-cancerous activity towards human breast cancer cell line (MCF7), with NC-G demonstrating enhanced pro-oxidant properties on MCF7 cells. The results from this study indicate that NC-G could be a potential nanomedicine as an antioxidant therapy in cardiovascular diseases or as pro-oxidant therapeutics in oncology.</div></div>","PeriodicalId":279,"journal":{"name":"Colloids and Surfaces B: Biointerfaces","volume":"254 ","pages":"Article 114798"},"PeriodicalIF":5.4,"publicationDate":"2025-05-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144068449","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Photo-activatable prodrug nanoparticles for reactive oxygen species amplification and cooperative cancer therapy 光活化的前药纳米粒子用于活性氧扩增和协同癌症治疗

IF 5.4 2区医学

Colloids and Surfaces B: Biointerfaces Pub Date : 2025-05-11 DOI: 10.1016/j.colsurfb.2025.114775

Xue Li , Xu Zhang , Haizhen Guo , Zhetao Li , Lei Han , Sheng Wang

{"title":"Photo-activatable prodrug nanoparticles for reactive oxygen species amplification and cooperative cancer therapy","authors":"Xue Li , Xu Zhang , Haizhen Guo , Zhetao Li , Lei Han , Sheng Wang","doi":"10.1016/j.colsurfb.2025.114775","DOIUrl":"10.1016/j.colsurfb.2025.114775","url":null,"abstract":"<div><div>Photodynamic therapy (PDT), as a minimally invasive cancer therapy, demonstrates certain advantages in treating superficial tumors. However, it often faces challenges such as low reactive oxygen species (ROS) generation efficiency and non-targeted distribution of photosensitizers. The combination of chemotherapy and PDT can address the limitations of single modal therapies and improve therapeutic outcomes. In this work, we design a prodrug-based nanomedicine that can achieve photo-activated cascade drug release. Under 660 nm laser irradiation, the generated singlet oxygen can trigger the release of chemotherapeutic agent chlorambucil, cinnamaldehyde and quinone methyl. Chlorambucil can exert anti-tumor effects and cinnamaldehyde can increase intracellular hydrogen peroxide levels, while quinone methyl can consume intracellular glutathione. This process ultimately results in the amplification of ROS signals and further activation of prodrugs. This nanomedicine exhibits the ability to amplify oxidative stress and potent anticancer activity. <em>In vivo</em> experiments show that the nanomedicine can effectively inhibit tumor growth. This work provides a promising mutually beneficial strategy for achieving cooperative cancer therapy.</div></div>","PeriodicalId":279,"journal":{"name":"Colloids and Surfaces B: Biointerfaces","volume":"253 ","pages":"Article 114775"},"PeriodicalIF":5.4,"publicationDate":"2025-05-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143942477","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Polypyrrole modified hFGF2-oil bodies for postsurgical melanoma recurrence suppression and wound healing acceleration 聚吡咯修饰hfgf2油体抑制黑色素瘤术后复发和促进创面愈合

IF 5.4 2区医学

Colloids and Surfaces B: Biointerfaces Pub Date : 2025-05-09 DOI: 10.1016/j.colsurfb.2025.114771

Shuwei Nie , Manru Wang , Zixuan Wang , Hang Yu , Zhuoyuan Li , Zhiqi Yang , Hongxiang Liu , Zheng Liu , Hongxia Ma , Xin Liu , Rui Chen , Yan Cheng

{"title":"Polypyrrole modified hFGF2-oil bodies for postsurgical melanoma recurrence suppression and wound healing acceleration","authors":"Shuwei Nie , Manru Wang , Zixuan Wang , Hang Yu , Zhuoyuan Li , Zhiqi Yang , Hongxiang Liu , Zheng Liu , Hongxia Ma , Xin Liu , Rui Chen , Yan Cheng","doi":"10.1016/j.colsurfb.2025.114771","DOIUrl":"10.1016/j.colsurfb.2025.114771","url":null,"abstract":"<div><div>Surgical treatment is the primary method for treating malignant melanoma at present. However, tumor recurrence after surgery and difficulty in wound healing remain significant challenges. This study designed and constructed a therapeutic wound dressing by loading polypyrrole (PPy) into human fibroblast growth factor 2 (hFGF2) covalently bonded camelina oil bodies (h-OB) to form Ph-OB. In a postoperative B16F10 melanoma model in C57BL/6 mice, the photothermal properties of PPy were utilized to increase the temperature at the surgical wound site through near-infrared light irradiation, performing photothermal therapy to kill residual tumors and inhibit tumor recurrence. Meanwhile, the release of hFGF2 from the Ph-OB acts on the postoperative wound site, promotes fibroblast proliferation and migration to accelerate wound healing. In summary, the developed Ph-OB not only prevents tumor recurrence but also facilitates the healing of surgery-induced wounds, showing great potential in postoperative cancer treatment.</div></div>","PeriodicalId":279,"journal":{"name":"Colloids and Surfaces B: Biointerfaces","volume":"253 ","pages":"Article 114771"},"PeriodicalIF":5.4,"publicationDate":"2025-05-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143942478","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Trapping/Monitoring of Toxic Arsenate in Human Skin Cell Lines: A Route for Preventing Skin Disorders 捕获/监测人体皮肤细胞系中有毒砷酸盐：预防皮肤疾病的途径

IF 5.4 2区医学

Colloids and Surfaces B: Biointerfaces Pub Date : 2025-05-09 DOI: 10.1016/j.colsurfb.2025.114773

Islam M. El-Sewify , Mohamed A. Shenashen , Moataz Mekawy , Mohamed S. Selim , Mohammed Y. Emran , Mohamed Khairy , Rasha F. El-Agamy , Mahmoud M. Selim , Ahmed Shahat , Mostafa M.H. Khalil , Ahmed Elmarakbi , Mitsuhiro Ebara , Sherif A. El-Safty

{"title":"Trapping/Monitoring of Toxic Arsenate in Human Skin Cell Lines: A Route for Preventing Skin Disorders","authors":"Islam M. El-Sewify , Mohamed A. Shenashen , Moataz Mekawy , Mohamed S. Selim , Mohammed Y. Emran , Mohamed Khairy , Rasha F. El-Agamy , Mahmoud M. Selim , Ahmed Shahat , Mostafa M.H. Khalil , Ahmed Elmarakbi , Mitsuhiro Ebara , Sherif A. El-Safty","doi":"10.1016/j.colsurfb.2025.114773","DOIUrl":"10.1016/j.colsurfb.2025.114773","url":null,"abstract":"<div><div>Prolonged exposure of human cells to toxic arsenate leads to significant skin diseases. To date, continuous and ultrasensitive assessments of toxic arsenate in water and human skin cell lines via simple trapping/monitoring technologies are urgently needed. Herein, nanomonitors based on self-organized multi-geode for trapping/monitoring ultra-trace arsenate concentrations (LOD=0.55ppb) in human skin cell lines were fabricated to prevent skin disorders. For the first time, the structural geode-like hierarchy was constructed via layer-by-layer assembly with nano/microscale features of wide pore openings and mesoporous cavities, enabling suitable diffusion, trapping, and binding of toxic arsenate in human skin cell lines. The biocompatible hierarchal geode with inner and outer receptor-functionalized surfaces revealed a fast in-vitro monitoring/trapping of arsenate in human skin cell lines in order of seconds, with a detection limit below the level affecting skin disorders. The nanomonitor's suitability for extracellular/intracellular tracking/monitoring/trapping toxic arsenate in human skin cell lines has been proven significantly under physiological conditions. Findings confirmed the exceptional characteristics of mesoscale geode-like pores for in-vitro trapping/quantifying toxic arsenate in human skin cell lines from the accumulation of carcinogenic toxicants, thus introducing a route for preventing skin disorders.</div></div>","PeriodicalId":279,"journal":{"name":"Colloids and Surfaces B: Biointerfaces","volume":"254 ","pages":"Article 114773"},"PeriodicalIF":5.4,"publicationDate":"2025-05-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144068450","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Advanced stem cell therapy using both cell spheroids transplant and paracrine factor release hydrogel patches for myocardial infarction 采用细胞球体移植和旁分泌因子释放水凝胶贴片的先进干细胞治疗心肌梗死

IF 5.4 2区医学

Colloids and Surfaces B: Biointerfaces Pub Date : 2025-05-09 DOI: 10.1016/j.colsurfb.2025.114772

Taekgwang Jeong , Min Suk Lee , Jin Jeon , Jin Hee Park , Youngdoo Chung , Hee Seok Yang

{"title":"Advanced stem cell therapy using both cell spheroids transplant and paracrine factor release hydrogel patches for myocardial infarction","authors":"Taekgwang Jeong , Min Suk Lee , Jin Jeon , Jin Hee Park , Youngdoo Chung , Hee Seok Yang","doi":"10.1016/j.colsurfb.2025.114772","DOIUrl":"10.1016/j.colsurfb.2025.114772","url":null,"abstract":"<div><div>Conventional micro-concave systems have been proposed as effective methods for facile cell spheroid formation, culture. However, these systems face challenges in terms of ease of cell transplantation and a low cell survival rate in ischemic disease. We present a novel open/close type hydrogel micro-concave patch (OC) designed for in situ 3D cell spheroid formation, culture, and a transplantable system utilizing a 3D printed mold. Open-type patches were fabricated with a rigid hydrogel, while closed-type patches were prepared with a combination of swellable soft hydrogel and rigid hydrogel. The open-type concave was intended for cell spheroid formation and subsequent transplantation into the ischemic region. Conversely, the close-type concave allowed released cytokines from cell spheroids, which were located inside the concave, to promote survival of transplanted cell spheroid. We hypothesized that transplant of open-type cell spheroids, combined with the release of paracrine factors from close-type cell spheroids, could enhance therapeutic outcomes in ischemic regions. The OC was prepared using different concentration ratios of swellable polyacrylamide (PAAM) hydrogel through 3D printed micropillar mold. Additionally, PAAM was characterized to enhance the compactness of close-type 3D cell spheroids. Transplantation of OC improved the therapeutic effect in a rat cardiac infarction model compared to open-type patches.</div></div>","PeriodicalId":279,"journal":{"name":"Colloids and Surfaces B: Biointerfaces","volume":"253 ","pages":"Article 114772"},"PeriodicalIF":5.4,"publicationDate":"2025-05-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143948702","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Liposomal encapsulation of crocin-rich tomato extract (Tomafran) and its in-depth evaluation as a cosmeceutical ingredient 富含藏红花素的番茄提取物（Tomafran）的脂质体包封及其作为药妆成分的深入评价

IF 5.4 2区医学

Colloids and Surfaces B: Biointerfaces Pub Date : 2025-05-08 DOI: 10.1016/j.colsurfb.2025.114766

Maria Mondéjar-López , María Paz García-Simarro , Julia Vega , Cristian Martínez Fajardo , Susana López-López , Oussama Ahrazem , Lourdes Gómez-Gómez , Felix L. Figueroa , Enrique Niza

{"title":"Liposomal encapsulation of crocin-rich tomato extract (Tomafran) and its in-depth evaluation as a cosmeceutical ingredient","authors":"Maria Mondéjar-López , María Paz García-Simarro , Julia Vega , Cristian Martínez Fajardo , Susana López-López , Oussama Ahrazem , Lourdes Gómez-Gómez , Felix L. Figueroa , Enrique Niza","doi":"10.1016/j.colsurfb.2025.114766","DOIUrl":"10.1016/j.colsurfb.2025.114766","url":null,"abstract":"<div><div>This study evaluates the potential of a genetically modified, crocin-rich tomato extract (Tomafran) as a biological photoprotector and for skin health applications. Biochemical characterization and antioxidant capacity of Tomafran were assessed. Tomafran showed lower values than saffron in the ABTS assay, but similar values in the DPPH and BCBA assays. Additionally, Tomafran reduced advanced glycation end products (AGEs) and reactive oxygen species (ROS) in human fibroblasts, which are related to the negative effects of UV radiation on the skin. The extract was encapsulated in liposomes, yielding particles with an average size of 60.96 nm, a polydispersity index (PDI) of 0.06, a zeta potential of −21.50 mV, and a spherical morphology. The liposomal formulation demonstrated storage stability and a controlled release profile, with approximately 60 % of the extract released within the first 10 hours. The photoprotective capacity, measured through sun protection factor (SPF) and other biological protection factors, showed very slight improvements with increasing concentrations of Tomafran, achieving values lower than 2. The extract showed instability against UV radiation and high temperature, although encapsulation in liposomes provided protection. The anti-inflammatory properties of the liposomal Tomafran extract were evaluated using RAW 264.7 macrophage cells, showing significant reductions in proinflammatory interleukins IL-6 and IL-12. These findings highlight Tomafran's potential for mitigating inflammation associated with oxidative stress and UV-induced skin damage.</div></div>","PeriodicalId":279,"journal":{"name":"Colloids and Surfaces B: Biointerfaces","volume":"253 ","pages":"Article 114766"},"PeriodicalIF":5.4,"publicationDate":"2025-05-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144068346","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0