Colloids and Surfaces B: Biointerfaces最新文献

{"title":"Nintedanib loaded iron (III) chelated melanin nanoparticles as an MRI-visible antifibrotic drug delivery system","authors":"Emirhan Bayrak ,&nbsp;Ece Bayir ,&nbsp;Engin Baysoy ,&nbsp;Alpay Özcan ,&nbsp;Bugra Ayan ,&nbsp;Ecem Saygili ,&nbsp;Gizem Kaleli-Can","doi":"10.1016/j.colsurfb.2025.114652","DOIUrl":"10.1016/j.colsurfb.2025.114652","url":null,"abstract":"<div><h3>Abstract</h3><div>Idiopathic pulmonary fibrosis (IPF) is a fatal, progressive lung disease characterized by extensive scarring and thickening of lung tissue that leads to respiratory failure. Early and accurate diagnosis is crucial for monitoring disease progression and assessing therapeutic efficacy. While imaging modalities such as radiological X-rays and high-resolution computed tomography (HRCT) are commonly employed, magnetic resonance imaging (MRI) offers significant advantages, including superior soft tissue contrast and the absence of ionizing radiation. However, in lung MRIs are hindered by short transverse relaxation times, low proton density, and motion artifacts which is addressed herein by developing theranostic platform combining MRI imaging with targeted drug delivery using melanin nanoparticles conjugated with nintedanib (MNP-NIN). Chelation with ferric ions (MNP-NIN-Fe³⁺) enhanced MRI visibility enabling non-invasive imaging and drug tracking. MNP-NIN and MNP-NIN-Fe³ ⁺ nanoparticles were built with mean diameters of 189 ± 44 nm and 182 ± 35 nm, respectively and demonstrating successful NIN conjugation. Controlled NIN release followed zero-order kinetics over 36 days. MNP conjugation reduced cytotoxicity in BEAS-2B and A549 cells improving the drug's safety. MRI experiments conducted with a 7.0 T animal scanner demonstrated enhanced imaging contrast with MNP-NIN-Fe solutions compared to saline underscoring their potential for localized visualization and tracking within lung tissues. By integrating MRI diagnostics and targeted drug delivery, the MNP-NIN-Fe³ ⁺ system offers a promising solution to overcome current challenges in IPF management. This theranostic platform addresses the limitations of conventional imaging techniques while providing an innovative strategy for reducing drug-related systemic side effects and improving therapeutic efficacy.</div></div>","PeriodicalId":279,"journal":{"name":"Colloids and Surfaces B: Biointerfaces","volume":"252 ","pages":"Article 114652"},"PeriodicalIF":5.4,"publicationDate":"2025-03-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143760495","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Chitin-binding protein behavior at chitosan interface studied by quartz crystal microbalance with dissipation monitoring (QCM-D): Binding quantification, orientation and affinity constants","authors":"Lisa Basso ,&nbsp;Clémence Vuillet ,&nbsp;Yvan Rahbé ,&nbsp;Laurent David ,&nbsp;Aurélia Charlot ,&nbsp;Guillaume Sudre","doi":"10.1016/j.colsurfb.2025.114650","DOIUrl":"10.1016/j.colsurfb.2025.114650","url":null,"abstract":"<div><div>A full comprehension of chitosan interaction with proteins is yet to be achieved, in view of the complexity of the physico-chemical behaviors found in the chitosan family, and the diversity of possible protein interaction mechanisms. In this work, we studied the interactions between chitosans and wheat germ agglutinin (WGA), a lectin that can bind chitin thanks to an amino-acid pattern called the hevein-like chitin-binding domain (CBD). The specificity of CBD interactions with chitin/chitosan chains and the impact of the degree of acetylation (DA) of chitosan have been studied by quartz crystal microbalance with dissipation monitoring (QCM-D). The mass per unit area of WGA adsorbed on chitosan was six times higher than that of immunoglobulin G (IGG), which does not contain a CBD. The mass per unit area of deposited WGA was also almost twice as high at higher chitosan DAs (54 %, 67 %, 76 %) than at lower DAs (0.5 %, 15 %, 35 %), evidencing more specific interaction with “chitin-like” chitosans. WGA is a dimer at neutral pH and presents 4 CBDs on each monomer. The repartition of these CBDs seems to allow for reorganization of WGA on the chitosan surface in order to favor the interactions of this chitosan with a higher number of proteins. Furthermore, the binding kinetics have been assessed and modelled with a two-step association model, providing insights on the observed association constants. These results indicate that QCM-D constitutes a suitable method for the analysis of lectin CBD–chitosan dynamic interactions and could be applied to other types of proteins, in particular CBD proteins or further used in biosensor elaboration or biomaterial coating assessment with chitosan of different DAs.</div></div>","PeriodicalId":279,"journal":{"name":"Colloids and Surfaces B: Biointerfaces","volume":"252 ","pages":"Article 114650"},"PeriodicalIF":5.4,"publicationDate":"2025-03-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143760492","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Cerium-doped carbon dots as dual-target agents against Alzheimer's β-amyloid fibrillogenesis and reactive oxygen species","authors":"Wenjuan Wang ,&nbsp;Zhaoyu Liu ,&nbsp;Hao Cheng ,&nbsp;Min Xu ,&nbsp;Zhi Du ,&nbsp;Wei Liu ,&nbsp;Chaofeng Zhang","doi":"10.1016/j.colsurfb.2025.114655","DOIUrl":"10.1016/j.colsurfb.2025.114655","url":null,"abstract":"<div><div>Both fibrillogenesis of amyloid β-protein (Aβ) and elevated levels of reactive oxygen species (ROS) contribute to the pathogenesis of Alzheimer's disease (AD). Beyond Aβ aggregation inhibition, the complexity necessitates the development of comprehensive therapeutic interventions for halting AD progression. Herein, a dual-target agent capable of Aβ aggregation inhibition and ROS scavenging was synthesized by doping cerium into carbon dots (Ce CDs). Ce CDs with a high Ce (III)/Ce (IV) ratio of 0.67 can scavenge various ROS, including superoxide anion radicals, hydroxyl radicals, hydrogen peroxide, and Aβ₄₀-induced ROS, thus mitigating cellular oxidative damage and rescuing cell viability. Additionally, Ce CDs present potent inhibition on Aβ₄₀ on-pathway fibrillization, disrupting the formation of highly ordered β-sheet structures and increasing cell viability from 50.2 % to 91.9 %. It is validated that the electrostatic interactions between Ce CDs and Aβ₄₀ are primarily responsible for preventing the conformational transition of Aβ₄₀ monomers. <em>In vivo</em> experiments with the transgenic <em>Caenorhabditis elegans</em> strain further validate the bifunctionality of Ce CDs in suppression of Aβ fibrillogenesis and attenuation of oxidative stress, thereby demonstrating the potential of combination therapy for AD. This finding highlights the important role of electrostatic interactions between Aβ and inhibitors in regulating Aβ aggregation, and provides new insights into the development of multifunctional agents for AD treatment.</div></div>","PeriodicalId":279,"journal":{"name":"Colloids and Surfaces B: Biointerfaces","volume":"252 ","pages":"Article 114655"},"PeriodicalIF":5.4,"publicationDate":"2025-03-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143725147","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Electrochemical detection of per- and polyfluoroalkyl substances: A review","authors":"Aashutosh Dube ,&nbsp;Shweta J. Malode ,&nbsp;Hanan Akhdar ,&nbsp;Abdullah N. Alodhayb ,&nbsp;Nagaraj P. Shetti","doi":"10.1016/j.colsurfb.2025.114653","DOIUrl":"10.1016/j.colsurfb.2025.114653","url":null,"abstract":"<div><div>Per- and poly-fluoroalkyl substances (PFASs) are recognised for their environmental persistence and bioaccumulation, necessitating a dependable detection technology. Traditional methods examine multiple facets. Electrochemical sensors represent a preferable alternative due to their reliability, real-time detection capabilities, and potential for on-site analysis. Metal-organic frameworks (MOFs) and molecularly imprinted polymers (MIPs) exhibit remarkable properties in analysis, including high sensitivity and selectivity, rapid response and efficient electron transfer capabilities. Nonetheless, the stability of MOFs occasionally poses issues in aquatic conditions. Utilising a microfluidic channel between interdigitated microelectrodes (IDμE) in a MOF-based electrochemical sensor for PFASa detection offers numerous advantages. It possesses a minimal limit of detection (LOD), comparable to cutting-edge ex-situ methodologies. The molecular interactions of the capture probes provide effective electrochemical transduction, while the nanoporous morphology of the materials and IDμE significantly enhance the signal-to-noise ratio. Extended diffusion durations impede detection abilities and limit molecular interactions between PFAS and electrode surfaces. The selectivity challenges involve differentiation problems and complex matrices. Accurately identifying PFAS compounds in samples is problematic, especially those with similar carbon chain lengths, and existing sensors are hindered by interference from non-fluorinated surfactants. Improvements in electrode design can be realised by the use of nonplanar interdigitated microelectrode arrays (NP-IDμE), the application of nanoporous and capacitive electrode technologies, and the incorporation of electrode nano-porosity to minimise non-specific adsorption. Improvements in signal and sensitivity can optimise the detection process. Signal increases can be attained by decoupling sensitivity and selectivity using force as a tuning parameter, employing ambient oxygen as a mediator molecule instead of expensive ferrocene, and utilising electrochemical impedance spectroscopy (EIS) for improved sensitivity. Integrating IoT with EC PFAS sensors indicates a promising future for environmental monitoring.</div></div>","PeriodicalId":279,"journal":{"name":"Colloids and Surfaces B: Biointerfaces","volume":"252 ","pages":"Article 114653"},"PeriodicalIF":5.4,"publicationDate":"2025-03-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143738279","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Functionalized hydroxypropyl-β-cyclodextrin inclusion complex for combined tumor therapy through intelligent delivery of paclitaxel and polarization of M2-like tumor associated macrophages","authors":"Jia Fu ,&nbsp;Wei Zhao ,&nbsp;Na Liang ,&nbsp;Shaoping Sun","doi":"10.1016/j.colsurfb.2025.114654","DOIUrl":"10.1016/j.colsurfb.2025.114654","url":null,"abstract":"<div><div>The treatment outcomes of chemotherapy are far from satisfactory due to suboptimal cellular uptake and inadequate intracellular release of antitumor drugs. Meanwhile, M2-like tumor associated macrophages (TAMs) in the tumor microenvironment promote cancer growth and metastasis. The combined strategy of chemotherapy and reprogramming M2-like TAMs within tumor microenvironment has emerged as a novel paradigm for cancer therapy. Hence, a pH-sensitive double-targeted inclusion complex was constructed for combined cancer therapy through the controlled paclitaxel (PTX) delivery and re-education of M2-like TAMs. The inclusion complex employed arginine and biotin modified hydroxypropyl-β-cyclodextrin (Arg-CD-Bio) as the host, with benzimidazole and mannose modified hyaluronic acid (BM-HA-Man) as the pH-sensitive plug. PTX was encapsulated in the inclusion complex. In vitro experiments indicated that the Arg-CD-Bio/BM-HA-Man inclusion complex could facilitate the specific release of PTX at the tumor site. The inclusion complex could be effectively internalized by M2-like TAMs and MCF-7 cells and further reprogramme M2-like TAMs to M1-like TAMs. In vivo antitumor therapy in 4T1 tumor-bearing mice had achieved remarkable suppression efficacy. These results suggested that the PTX-loaded Arg-CD-Bio/BM-HA-Man inclusion complex was a promising platform for efficient cancer treatment.</div></div>","PeriodicalId":279,"journal":{"name":"Colloids and Surfaces B: Biointerfaces","volume":"252 ","pages":"Article 114654"},"PeriodicalIF":5.4,"publicationDate":"2025-03-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143734953","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Properties and potential uses of a biosurfactant extract obtained from corn steep water by a dialysis process","authors":"A. Martínez-Arcos ,&nbsp;M. Reig ,&nbsp;J.M. Cruz ,&nbsp;J.L. Cortina ,&nbsp;B. Pérez-Cid ,&nbsp;A.B. Moldes ,&nbsp;X. Vecino","doi":"10.1016/j.colsurfb.2025.114649","DOIUrl":"10.1016/j.colsurfb.2025.114649","url":null,"abstract":"<div><div>There is currently a significant demand for more sustainable and compatible ingredients and additives not only for cosmetic and pharmaceutical formulations but also for applications within the agrifood industry. This demand is emphasized by regulations like the COSMOS-standard, which classifies, for instance, cosmetic formulations based on ingredients that meet stringent criteria for their downstream processes promoting physical separation. In this research, a biosurfactant extract derived from a naturally fermented stream within the corn-wet milling industry was obtained for the first time through physical separation methods based on a dialysis process at lab scale. This was done as an alternative to the use of organic solvents. Subsequently, the extract underwent comprehensive characterization and assessment to ascertain its efficacy as a solubilizer, emulsifier, and spreading and wetting agent. The findings revealed that this biosurfactant extract is composed of glycopeptides and peptides, like the biosurfactant extract produced by lactic acid bacteria, but with lower critical micellar concentration (CMC). Moreover, the biosurfactant extract exhibited non-irritating properties (Mucosal Irritation Score &lt; 0.07) and demonstrated interesting capabilities as a booster in the formulation of Pickering emulsions. Furthermore, it demonstrated a spreading capacity comparable to that of sodium dodecyl sulphate (SDS); but not a good solubilization of chicken broth cubes. However, its most noteworthy characteristic was its remarkable wettability capacity, which holds significant promise for applications in the cosmetic industry as a humectant agent, aligning with the standards set in COSMOS standard.</div></div>","PeriodicalId":279,"journal":{"name":"Colloids and Surfaces B: Biointerfaces","volume":"252 ","pages":"Article 114649"},"PeriodicalIF":5.4,"publicationDate":"2025-03-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143716086","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Investigations into the topical hemostatic and wound healing efficacy of binary Hyaluronic acid/Collagen membranes coated with tranexamic acid and vitamin K; InVitro","authors":"Wareef H Alnawwar ,&nbsp;Abeer Abdulaziz H. Bukhari","doi":"10.1016/j.colsurfb.2025.114647","DOIUrl":"10.1016/j.colsurfb.2025.114647","url":null,"abstract":"<div><div>A hemostatic dressing refers to a specific category of medical dressings that have been developed for managing and controlling bleeding. A synthesized hyaluronic acid/collagen (HA/COL) membrane integrated with tranexamic acid (TRAX) and/or vitamin K (Vit K) using the lyophilization technique is fabricated, followed by physical, chemical, and biological assessment. The clotting time and coagulation variables, as prothrombin (PT), partial thromboplastin time (PPT), and international normalized ratio (INR), were also assessed. The antimicrobial properties were tested against Gram-positive &amp; Gram-negative bacteria, yeast, and fungi. The obtained membranes exhibited porous and swellable characteristics, demonstrating their ability to encapsulate vitamin K and/or tranexamic acid effectively. The cell studies demonstrated that the samples containing vitamin K exhibited less toxicity, in contrast to the TRAX compounds. The assessment of cytotoxicity is substantiated by conducting research on wound healing and determining the IC50 values. Additionally, the samples of vitamin K exhibited rapid clotting, which was corroborated by assessing the coagulation blood factors such as PT, PTT, and INR. The antibacterial activity seen in all samples can be attributed to the presence of HA among the membrane constituents. It is advisable to integrate vitamin K into the HA/COL membrane rather than within TRAX or a combination of TRAX.</div></div>","PeriodicalId":279,"journal":{"name":"Colloids and Surfaces B: Biointerfaces","volume":"252 ","pages":"Article 114647"},"PeriodicalIF":5.4,"publicationDate":"2025-03-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143734954","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Electrospun amine-modified polysuccinimide/polycaprolactone nanofiber hydrogel dressings as antibacterial and hemostatic wound dressing applications","authors":"Jialu Pan ,&nbsp;Qi Meng ,&nbsp;Li Zhang ,&nbsp;Caidan Zhang ,&nbsp;Shaohua Wu ,&nbsp;Huiyuan Zhai","doi":"10.1016/j.colsurfb.2025.114648","DOIUrl":"10.1016/j.colsurfb.2025.114648","url":null,"abstract":"<div><div>Design and construction of novel dressings with appropriate structure and multiple bio-functions are urgently required for the wound treatment applications. In this study, a series of wound dressings composed of amine-modified polysuccinimide (PSI)/polycaprolactone (PCL) hydrogel nanofibers were fabricated by using the conjugated electrospinning and the chemical crosslinking post-treatment. All the dressings with different PSI/PCL ratios could effectively maintain the fibrous morphology even after the chemical crosslinking process. The ultimate stress and Young's modulus of the PSI-NH₂/PCL dressings significantly increased with higher PCL content. In particular, when the proportion of PCL reached 40 % (with a PSI/PCL mass ratio of 60/40), the PSI-NH₂/PCL dressing exhibited the most excellent mechanical properties, with a Young's modulus of 31.9 ± 8.9 MPa and an ultimate stress of 7.4 ± 0.4 MPa, which were significantly higher than the other samples. Furthermore, all samples exhibited a high swelling ratio of over 350 %, although a noticeable decrease in swelling properties was observed when the content of PCL increased. In addition, all the PSI-NH₂/PCL dressings showed a high antibacterial activity (&gt; 99 %) against both <em>Escherichia coli</em> (<em>E. coli</em>) and <em>Staphylococcus aureus</em> (<em>S. aureus</em>), which demonstrated that the addition of PCL has no significant influence on the antibacterial activity of the PSI. Moreover, all the PSI-NH₂/PCL nanofiber hydrogel dressings had excellent biocompatibility to human dermal fibroblasts (HDFs). Importantly, all the PSI-NH₂/PCL nanofiber hydrogel dressings presented excellent hemostatic capacity. Notably, even the dressing with the highest PCL content still demonstrated a 71.8 % reduction in bleeding compared to the control group. In summary, our PSI-NH₂/PCL nanofiber hydrogel dressings with high absorption, biocompatibility and extracellular matrix (ECM)-mimicking capacities, as well as great mechanical, antibacterial, and hemostatic properties are excellent as a promising wound dressing.</div></div>","PeriodicalId":279,"journal":{"name":"Colloids and Surfaces B: Biointerfaces","volume":"252 ","pages":"Article 114648"},"PeriodicalIF":5.4,"publicationDate":"2025-03-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143680036","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"All-in-one multifunctional tri-block glycopolymers for targeted delivery of cisplatin and cancer chemotherapy","authors":"Sichen Li ,&nbsp;Difei Zhang ,&nbsp;Yi Li,&nbsp;Jingjing Zhou,&nbsp;Jinghua Chen,&nbsp;Yan Zhang","doi":"10.1016/j.colsurfb.2025.114639","DOIUrl":"10.1016/j.colsurfb.2025.114639","url":null,"abstract":"<div><div>Cisplatin (CDDP) as a first-line chemotherapy drug has long suffered drawbacks of severe side effects and poor pharmacokinetics. Thus, various nano-carriers of complex architectures and multi-step modifications have been developed to overcome these challenges, but a simple delivery system with integrated multi-functions is still in demand. Herein, we synthesized a new type of glyco-based triple hydrophilic block copolymers for the delivery of CDDP and thereof cancer therapy. The incorporated glucuronic acid part is complexed with CDDP with high entrapment efficiency, while providing pH-responsive release in the acidic tumor microenvironment. The galactose segment is implanted for liver-targeting effect, which can also significantly lower the side effects of CDDP. As a result, the CDDP-loaded nanoparticle <strong>PGG2/Pt</strong> showed selectively faster endocytosis rates into HepG2 cells in vitro, with the calculated IC₅₀ value even comparable to that of free CDDP. The in vivo experiments on a HepG2-bearing mouse model, <strong>PGG2/Pt</strong> showed excellent anti-tumor activity and enhanced drug accumulation on tumor, together with much lowered nephrotoxicity, hepatotoxicity, and splenic toxicity. This work provides a new strategy for CDDP delivery with higher loading efficiency as well as biosafety.</div></div>","PeriodicalId":279,"journal":{"name":"Colloids and Surfaces B: Biointerfaces","volume":"252 ","pages":"Article 114639"},"PeriodicalIF":5.4,"publicationDate":"2025-03-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143680034","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Tuning structure and morphology of lipidic cubosomes by encapsulation of novel porphyrin-derivatives","authors":"Valeria D’Annibale ,&nbsp;Leonardo Ariodante ,&nbsp;Claudia Marconi ,&nbsp;Luca Piccirillo ,&nbsp;Peter Jönsson ,&nbsp;Andrea D’Annibale ,&nbsp;Donato Monti ,&nbsp;Anita Scipioni ,&nbsp;Karin Schillén ,&nbsp;Luciano Galantini ,&nbsp;Marco Fornasier","doi":"10.1016/j.colsurfb.2025.114646","DOIUrl":"10.1016/j.colsurfb.2025.114646","url":null,"abstract":"<div><div>Cubosomes are non-lamellar lipid nanoparticles that have drawn a significant attention in the field of nanomedicine due to their tunable properties. However, the formation of vesicles during the preparation of cubosomes, and the presence of mixed bicontinuous cubic phases, may lead to artifacts and lack of correlation between the physico-chemical and biological characterization. In this work, we have formulated cubosomes composed by monoolein as building block and triblock copolymer Pluronic® F108 as a stabilizer, encapsulating three porphyrin derivatives: two attached to bile acid moieties and one to a tetrapeptide to be used for potential theranostic applications. First, the effect of the cargo concentration (0.25, 0.50 and 1.00 mg/mL, for all three molecules) was evaluated on the structure, showing that the bile acid derivatives did not affect the self-assembly of the lipid providing only <em>Pn3m</em> phases; however, a mixed phase <em>Pn3m</em> + <em>Im3m</em> and a subsequent loss in crystallinity were induced by increasing concentrations of the tetrapeptide derivative. Overall, the encapsulation of the three molecules at 25 and 37 ^∘C did not affect neither the hydrodynamic size nor the polydispersity of the cubosomes, influencing mainly the <em>ζ</em>-potential - positive in the case of the tetrapeptide and negative for the bile acid derivatives. The samples formulated with 0.50 mg/mL exhibited higher colloidal stability over time, with no significant changes in size or <em>ζ</em>-potential for over a month. Interestingly, the formulations containing the bile acid derivatives displayed the typical morphology of cubosomes in solution and a reduced number of vesicles (ca. 60:40 as cubosomes-to-vesicles ratio), whereas the sample containing the porphyrin attached to the tetrapeptide led to a ratio of cubosomes-to-vesicles estimated as 26:74, similar to the results of the empty formulation. The experiments at body temperature highlighted that the structure of the different formulations was not affected in a significant manner with retention of the phases observed at room temperature. The promising physico-chemical properties, especially at body temperature, could make these samples suitable as nanoplatforms for drug delivery applications.</div></div>","PeriodicalId":279,"journal":{"name":"Colloids and Surfaces B: Biointerfaces","volume":"252 ","pages":"Article 114646"},"PeriodicalIF":5.4,"publicationDate":"2025-03-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143734952","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}