Colloids and Surfaces B: Biointerfaces最新文献

{"title":"Prolonged local retention of vancomycin achieved by a multivesicular liposomes in thermoresponsive gel system for the prevention and treatment of intervertebral disc infection","authors":"Zhencheng Gao ,&nbsp;Hongxia Fan ,&nbsp;Lutong He ,&nbsp;Yu Zhang ,&nbsp;Tian Yin ,&nbsp;Haibing He ,&nbsp;Xing Tang ,&nbsp;Yanjiao Wang ,&nbsp;Jingxin Gou","doi":"10.1016/j.colsurfb.2025.114593","DOIUrl":"10.1016/j.colsurfb.2025.114593","url":null,"abstract":"<div><div>The therapeutic efficacy of intervertebral disc (IVD) infections treated with intravenous vancomycin (VCM) is often limited by inadequate blood supply to the IVD. In this study, we developed a localized and sustained-release drug delivery system for the intradiscal administration of VCM. First, VCM-loaded multivesicular liposomes (VCM-MVLs) were prepared using a two-step emulsification process, and we investigated the effects of the preparation process and formulation composition on the quality of the MVLs. The prepared MVLs exhibited an encapsulation efficiency of 92.08 ± 6.44 %, a particle size of 30.35 μm, and a sustained release over 12 days. Subsequently, VCM-MVLs were incorporated with PLGA-PEG-PLGA (PPP) to create a thermosensitive hydrogel composite formulation (VCM-MVL-PPP), which demonstrated a gelling temperature of 28.4°C and exhibited prolonged sustained release over 20 days. The antimicrobial activity of VCM-MVL-PPP was maintained for an extended duration of 21 days. Additionally, the VCM-MVL-PPP demonstrated an optimal retention capacity of 14 days in the <em>in vivo</em> retention assay. <em>In vivo</em> safety results confirmed the high biocompatibility and negligible toxicity of the formulation. In conclusion, the presence of PPP as a secondary release barrier for the drug released from MVLs allowed VCM-MVL-PPP to exhibit prolonged drug release and retention in the IVDs compared to VCM-MVLs. Our findings provide a valuable reference for peptide delivery and the treatment of disc infections.</div></div>","PeriodicalId":279,"journal":{"name":"Colloids and Surfaces B: Biointerfaces","volume":"251 ","pages":"Article 114593"},"PeriodicalIF":5.4,"publicationDate":"2025-02-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143488429","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Selenium nanoparticles as catalysts for nitric oxide generation","authors":"Shu Geng ,&nbsp;Yingzhu Zhou ,&nbsp;Gervase Ng ,&nbsp;Qingqing Fan ,&nbsp;Soshan Cheong ,&nbsp;Federico Mazur ,&nbsp;Cyrille Boyer ,&nbsp;Rona Chandrawati","doi":"10.1016/j.colsurfb.2025.114592","DOIUrl":"10.1016/j.colsurfb.2025.114592","url":null,"abstract":"<div><div>The critical role of nitric oxide (NO), a potent signalling molecule, in various physiological processes has driven the development of NO delivery strategies for numerous therapeutic applications. However, NO’s short half-life poses a significant challenge for its effective delivery. Glutathione peroxidase, a selenium-containing antioxidant enzyme, can catalyse the decomposition of <em>S</em>-nitrosothiols (endogenous NO prodrugs) to produce NO <em>in situ</em>. Inspired by this, we explored selenium nanoparticles (SeNPs) for their enzyme-mimicking NO-generating activity. Stabilised with polyvinyl alcohol (PVA) or chitosan (CTS), SeNPs demonstrated tuneable NO generation when exposed to varying concentrations of NO prodrug, nanoparticles, and glutathione (GSH). In the presence of GSH, a naturally occurring antioxidant in the human body, 0.1 µg mL⁻¹ of SeNPs could catalytically generate 7.5 µM of NO under physiological conditions within 30 min. We investigated the effects of nanoparticle crystallinity and NO prodrug type on NO generation, as well as the stability and sustained NO generation of the catalytic nanoparticles. PVA-stabilised SeNPs were non-toxic to NIH 3T3 cells and effectively dispersed <em>Pseudomonas aeruginosa</em> biofilms upon NO generation. This study broadens the repertoire of nanomaterials for NO generation and highlights SeNPs as a non-toxic alternative for therapeutic NO delivery.</div></div>","PeriodicalId":279,"journal":{"name":"Colloids and Surfaces B: Biointerfaces","volume":"251 ","pages":"Article 114592"},"PeriodicalIF":5.4,"publicationDate":"2025-02-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143527461","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Oral functional protein Z: Mitigation of thrombosis via thrombin inhibition to prevent cardiovascular disease","authors":"Chen Xu ,&nbsp;Hanhan Liu ,&nbsp;Mingyang Sun,&nbsp;Yang Gao,&nbsp;Tuo Zhang,&nbsp;Guanghua Zhao,&nbsp;Chenyan Lv","doi":"10.1016/j.colsurfb.2025.114569","DOIUrl":"10.1016/j.colsurfb.2025.114569","url":null,"abstract":"<div><div>Thrombin, a serine protease, plays a crucial role in thrombosis and is considered a significant target for the treatment of thrombotic diseases. This study aimed to investigate the malt-derived serine protease inhibitor protein Z (PZ) as an oral food enrichment for inhibiting thrombosis. The ability of PZ to withstand gastrointestinal digestion was assessed through in vitro simulated gastrointestinal digestion experiments. Its effective ability to traverse the digestive tract was demonstrated in vivo experiments. To explore its antithrombotic mechanism, the antithrombin activity of PZ was evaluated using two thrombin substrates, suggesting that it may inhibit either the active site or exosite 1 of thrombin. Additionally, the half-life of PZ was 10.76 ± 0.45 min, indicating its favorable pharmacokinetic profile. The anticoagulant activity and antithrombotic effects of PZ were further assessed using a mice tail thrombosis model induced by κ-carrageenan. The black tail rate in the PZ group is 51.23 ± 1.72 % lower than that of the model group (71.87 ± 5.90 %). These results demonstrated that PZ significantly inhibited thrombosis, with its physiological mechanism linked to the coagulation pathway, particularly through the inhibition of thrombin activity. Therefore, PZ has the potential to be developed as an oral antithrombotic food enrichment.</div></div>","PeriodicalId":279,"journal":{"name":"Colloids and Surfaces B: Biointerfaces","volume":"251 ","pages":"Article 114569"},"PeriodicalIF":5.4,"publicationDate":"2025-02-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143478749","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Formulation and heat stability of self-assembled O/W food-grade microemulsions formulated with bile salts or tween 80 as surfactants","authors":"Liliana Piñon-Gómez ,&nbsp;Néstor Gutiérrez-Méndez ,&nbsp;José C. Rodríguez-Figueroa ,&nbsp;Martha Y. Leal-Ramos ,&nbsp;Maria R. Peralta-Pérez ,&nbsp;Martha Ruiz-Gutiérrez ,&nbsp;León R. Hernández-Ochoa ,&nbsp;Diego E. Carballo-Carballo ,&nbsp;Blanca E. Sánchez-Ramírez","doi":"10.1016/j.colsurfb.2025.114589","DOIUrl":"10.1016/j.colsurfb.2025.114589","url":null,"abstract":"<div><div>Recently, there has been a growing interest in developing food-grade (F-G) microemulsions (MEMs) with potential applications in the food industry, such as a green solvent for extracting bioactive compounds or as a vehicle to solubilize low-polar bioactive compounds. Therefore, this work aimed to create F-G MEMs using a low-energy titration method, bile salt or tween 80 as surfactants, and F-G ingredients like acetic acid, olive oil, limonene, and coconut oil. For this purpose, 56 formulations were assessed, from which 14 created oil-in-water (O/W) one-phase Winsor type IV microemulsions. All the F-G MEMs were transparent (turbidity ≤ 0.17 %) with watery densities (1.00 – 1.034 g/cm³) and viscosities (∼1–5 mPa･s). The MEMs formulated with bile salts had a neutral pH (7.75) and could only solubilize small lipids like limonene (2 % maximum). In contrast, those MEMs using tween 80 as surfactant showed an acidic pH (∼1–5.9) and could solubilize triglyceride-based lipids (0.2 % maximum) and limonene (4 % maximum) at surfactant concentrations as low as 1 %. The F-G MEMs were successfully created using the low-energy titration method, although the time to form each MEM varied significantly. The MEMs formulated with bile salts were self-assembled in ∼12 h, whereas the formation of MEMs with tween 80 required up to 28 days. Nevertheless, once the MEMs were formed, they presented extraordinary stability to high-shear stirring (15,600 rpm for 5 min) and heating (up to 150 ºC) without turning cloudy or showing a phase separation.</div></div>","PeriodicalId":279,"journal":{"name":"Colloids and Surfaces B: Biointerfaces","volume":"251 ","pages":"Article 114589"},"PeriodicalIF":5.4,"publicationDate":"2025-02-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143465487","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Synergistic effects of paclitaxel and platelet-superparamagnetic iron oxide nanoparticles for targeted chemo-hyperthermia therapy against breast cancer","authors":"Mohamadreza Tavakoli ,&nbsp;Samane Maghsoudian ,&nbsp;Amir Rezaei-Aderiani ,&nbsp;Maliheh Hajiramezanali ,&nbsp;Yousef Fatahi ,&nbsp;Mahdiyar Amani ,&nbsp;Elham Sharifikolouei ,&nbsp;Mohammad Hossein Ghahremani ,&nbsp;Mohammad Raoufi ,&nbsp;Hamidreza Motasadizadeh ,&nbsp;Rassoul Dinarvand","doi":"10.1016/j.colsurfb.2025.114584","DOIUrl":"10.1016/j.colsurfb.2025.114584","url":null,"abstract":"<div><div>Due to the limited therapeutic efficacy and side effects associated with conventional chemotherapy, researchers have turned their attention to developing targeted drug delivery systems using advanced nanotechnology. Coating nanoparticles (NPs) with cell membranes is a promising strategy because it extends their circulation times and allows them to selectively adhere to damaged vessel sites through the platelet membrane surface, thereby enhancing tumor uptake. Herein, we have developed a biomimetic drug delivery system consisting of superparamagnetic iron oxide nanoparticles (SPIONs) coated by platelet membranes (PM) for carrying Paclitaxel (PTX) to exploit the synergism effect of chemotherapy and magnetic hyperthermia. Controlled-release PTX nanoparticles exhibited consistent behavior over time, indicating no significant difference in release between SPION/PTX and SPION/PTX/PM at pH 7.4. However, at pH 5.5, improved release was observed, specifically a 1.4-fold increase for SPION/PTX/PM. The confocal and flow cytometry results showed an enhancement in the cellular uptake of SPION/PTX/PM nanoparticles, with an average fluorescence intensity of 142 ± 12.5. MTT results showed superior cytotoxic effects for SPION/PTX/PM compared to SPION/PTX and free PTX, showing an IC50 value of 5 μg/mL after 48 h of treatment. Furthermore, the IC50 decreased to 1 μg/mL when an alternating magnetic field was applied. Hence, the <em>in vivo</em> results and histopathological staining showed that the SPION/PTX/PM-AMF treatment group exhibited the highest rate of tumor growth inhibition, reaching nearly 92.14 %. These findings highlight the potential of using platelet membrane-coated nanoparticles for targeted delivery, combining magnetic hyperthermia and chemotherapy to minimize chemotherapy's undesirable effects while maximizing therapeutic outcomes.</div></div>","PeriodicalId":279,"journal":{"name":"Colloids and Surfaces B: Biointerfaces","volume":"251 ","pages":"Article 114584"},"PeriodicalIF":5.4,"publicationDate":"2025-02-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143478750","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Biomimetic nanocrystals co-deliver paclitaxel and small-molecule LF3 for ferroptosis-combined chemotherapy for gastric cancer","authors":"Qianqian Zhao ,&nbsp;Ting Wu ,&nbsp;Chunming Tang ,&nbsp;Jie Li ,&nbsp;Min Wu ,&nbsp;Jie Wu ,&nbsp;Zhiji Wang ,&nbsp;Yinxin Zhu ,&nbsp;Huae Xu ,&nbsp;Xiaolin Li","doi":"10.1016/j.colsurfb.2025.114586","DOIUrl":"10.1016/j.colsurfb.2025.114586","url":null,"abstract":"<div><div>Combination chemotherapy is considered more effective than monotherapy in enhancing clinical outcomes. Ferroptosis, a unique form of regulated cell death, has been demonstrated to inhibit tumor growth and progression. Consequently, combining ferroptosis with chemotherapy represents a promising and innovative approach to antitumor therapy. In this study, we developed a novel TMTP1-modified biomimetic nanocrystal (TRNC@P + L) for the co-delivery of PTX and LF3, aiming to achieve ferroptosis-combined chemotherapy in gastric cancer. TRNC@P + L, which incorporates a tumor-homing peptide-modified red blood cell membrane, demonstrated efficient tumor targeting, prolonged circulation, enhanced drug bioavailability, and reduced non-specific toxicities of free PTX and LF3. By utilizing the synergistic effects of PTX and LF3, TRNC@P + L combination therapy significantly inhibited tumor growth, as demonstrated by both in vitro and in vivo studies. Mechanistically, TRNC@P + L triggers ferroptosis in tumor cells by downregulating GPX4 expression, the promotion of ROS accumulation, and the enhancement of lipid peroxidation. These processes synergistically enhance the anticancer efficacy of PTX.</div></div>","PeriodicalId":279,"journal":{"name":"Colloids and Surfaces B: Biointerfaces","volume":"251 ","pages":"Article 114586"},"PeriodicalIF":5.4,"publicationDate":"2025-02-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143478752","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"GRP94 mediates blood-brain barrier permeation and substantia nigra-specific drug distribution in Parkinson’s disease","authors":"Ziyan Lv ,&nbsp;Yuteng Zeng ,&nbsp;Taiyong Lv ,&nbsp;Qiao Liu ,&nbsp;Liang Han","doi":"10.1016/j.colsurfb.2025.114585","DOIUrl":"10.1016/j.colsurfb.2025.114585","url":null,"abstract":"<div><div>Parkinson’s disease is a progressive neurodegenerative disorder, which is characterized by pathological changes and progressive loss of dopaminergic neurons in the substantia nigra, e.g., endoplasmic reticulum stress. The blood-brain barrier (BBB) restricts the intracranial drug concentration and the therapeutic outcomes to a remarkable degree. Receptor-mediated transport has been extensively leveraged to design brain-targeting drug delivery systems to enhance intracranial drug levels. However, the target receptors at the BBB are widely expressed in normal brain parenchymal cells, which would affect drug distribution in local diseased brain regions, e.g., the substantia nigra in Parkinson’s disease. Here, we found glucose-regulated protein 94 (GRP94), as an endoplasmic reticulum resident protein, is upregulated at the surface of substantia nigra neurons in Parkinson’s disease. Considering that GRP94 could function like receptors to trigger cellular endocytosis and transcytosis, we constructed GRP94-specific peptide ligand NGPTHE-modified N-NPs and found that N-NPs could specially deliver drugs to the locally affected substantia nigra in Parkinson’s disease. This study provides a GRP94-based proof-of-concept strategy for specific drug delivery for Parkinson’s disease.</div></div>","PeriodicalId":279,"journal":{"name":"Colloids and Surfaces B: Biointerfaces","volume":"250 ","pages":"Article 114585"},"PeriodicalIF":5.4,"publicationDate":"2025-02-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143454458","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"3D-bioprinting of MXenes: Developments, medical applications, challenges, and future roadmap","authors":"Muhammad Ikram ,&nbsp;M.A. Parvez Mahmud ,&nbsp;Amina Akbar Kalyar ,&nbsp;Thamer Alomayri ,&nbsp;Albandary Almahri ,&nbsp;Dilshad Hussain","doi":"10.1016/j.colsurfb.2025.114568","DOIUrl":"10.1016/j.colsurfb.2025.114568","url":null,"abstract":"<div><div>MXenes is a member of 2D transition metals carbides and nitrides with promising application prospects in energy storage, sensing, nanomedicine, tissue engineering, catalysis, and electronics. In the current era, MXenes have been widely applied in biomedical applications due to their unique rheological and electrochemical attributes. They have a larger surface area with more active sites, higher conductivity, lower cytotoxicity, and greater biocompatibility, making them highly suitable candidates for in-vivo biomedical applications. Due to recent advancemnets in MXenes 3D bioprinting, they are widely applied in regenerative medicine to combat challenges in suitable transplantation of tissues and organs. However, 3D bioprinting of MXenes has several complexities based on cell type, cytotoxicity, cell viability, and differentiation. To address these intricacies, surface modifications of MXene materials are done, which makes them highly fascinating for the 3D printing of tissues and organs. In the current review, we summarized recent progress in 3D bioprinting of MXene materials to construct scaffolds with desired rheological and biological properties, focusing on their potential applications in cancer phototherapy, tissue engineering, bone regeneration, and biosensing. We also discussed parameters affecting their biomedical applications and possible solutions by applying surface modifications. In addition, we addressed current challenges and future roadmaps for 3D bioprinting of MXene materials, such as generating high throughput 3D printed tissue constructs, drug delivery, drug discovery, and toxicology.</div></div>","PeriodicalId":279,"journal":{"name":"Colloids and Surfaces B: Biointerfaces","volume":"251 ","pages":"Article 114568"},"PeriodicalIF":5.4,"publicationDate":"2025-02-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143510439","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Inhalable lipid-based nanocarriers covered by polydopamine for effective mucus penetration and pulmonary retention","authors":"Xiaobo Zhang ,&nbsp;Zhengli Zhou ,&nbsp;Xiaohui Yang ,&nbsp;Lei Huang,&nbsp;Qin Wang,&nbsp;Yi Chen,&nbsp;Kesi Du,&nbsp;Jianqing Peng","doi":"10.1016/j.colsurfb.2025.114576","DOIUrl":"10.1016/j.colsurfb.2025.114576","url":null,"abstract":"<div><div>To overcome the critical challenge in drug inhalation for pulmonary diseases, we innovatively proposed that polydopamine (PDA) as a surface modification material had great potential to improve the mucus permeation and pulmonary retention of inhalable lipid-based nanocarriers. We prepared PDA coated lipid nanoemulsions/solid lipid nanoparticles/liposomes and systematically evaluated their interactions with mucin and pulmonary retention after inhalation. PDA-coated lipid-based nanocarriers exhibited weaker interactions with mucins, higher mucus permeability and cellular uptake by the respiratory epithelium cells compared to PEGylated lipid-based nanocarriers. However, the pulmonary retention advantage of PDA coating was shown in lipid nanoemulsions (&lt; 50 nm) and solid lipid nanoparticles (&lt; 100 nm). Liposomes (∼ 150 nm) with PEGylation possessed higher pulmonary retention than that coated by PDA. It was suggested that PEGylated liposomes were liable to be phagocytosed by alveolar macrophages due to binding with specific antibodies. Overall, this work suggests that PDA as a surface modification material of inhalable lipid-based nanocarriers holds promise for effective mucus penetration and pulmonary retention.</div></div>","PeriodicalId":279,"journal":{"name":"Colloids and Surfaces B: Biointerfaces","volume":"251 ","pages":"Article 114576"},"PeriodicalIF":5.4,"publicationDate":"2025-02-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143465486","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A novel NIR-dependent IDO-inhibiting ethosomes treatment melanoma through PTT/PDT/immunotherapy synergy","authors":"Changzhen Sun ,&nbsp;Qingqing He ,&nbsp;Xun Yang ,&nbsp;Jianv Wang ,&nbsp;Dengmei Xia ,&nbsp;Tong Xia ,&nbsp;Hongye Liao ,&nbsp;Xia Xiong ,&nbsp;Yongmei Liao ,&nbsp;Hongping Shen ,&nbsp;Qin Sun ,&nbsp;Yuan Yuan ,&nbsp;Yuanmin He ,&nbsp;Li Liu","doi":"10.1016/j.colsurfb.2025.114565","DOIUrl":"10.1016/j.colsurfb.2025.114565","url":null,"abstract":"<div><div>Phototherapy is a treatment method that uses the characteristics of different bands of light to treat diseases. Tumor immunotherapy, on the other hand, treats tumors by regulating the body’s immune system. The combination of phototherapy and immunotherapy can significantly enhance the treatment of melanoma. In this study, we prepared and characterized INEs, a novel ethosome composed of the photosensitizer IR251 and the Indoleamine-2, 3-dioxygenase (IDO) inhibitor NLG919. INEs demonstrated excellent phototherapeutic properties, strong phototoxicity, and a notable ability to inhibit IDO. Under 808 nm laser irradiation, INEs effectively induced immunogenic cell death (ICD) in melanoma cells. <em>In vivo</em> experiments demonstrated that INEs injection into primary tumors triggered ICD, promoted maturation of DC cells, and activated naive T cells, leading to the production of effector T cells (specifically CD4⁺ and CD8⁺ T cells) that targeted and killed tumor cells. Both primary and distant tumors were treated simultaneously with favorable biosafety. In conclusion, INEs represent a promising strategy for melanoma treatment by a combination of phototherapy and immunotherapy with high safety. This study provides new insights and a theoretical basis for the clinical treatment of melanoma.</div></div>","PeriodicalId":279,"journal":{"name":"Colloids and Surfaces B: Biointerfaces","volume":"251 ","pages":"Article 114565"},"PeriodicalIF":5.4,"publicationDate":"2025-02-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143473969","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}