Colloids and Surfaces B: Biointerfaces最新文献

{"title":"Probiotics-embedded polymer films for oral health: Development, characterization, and therapeutic potential","authors":"Charlotte Eckermann ,&nbsp;Christof Johannes Klein ,&nbsp;Florian Schäfer ,&nbsp;Marc Thiel ,&nbsp;Agnes-Valencia Weiss ,&nbsp;Christian Motz ,&nbsp;Karen Lienkamp ,&nbsp;Matthias Hannig ,&nbsp;Marc Schneider","doi":"10.1016/j.colsurfb.2025.114886","DOIUrl":"10.1016/j.colsurfb.2025.114886","url":null,"abstract":"<div><div>The oral microbiome plays a crucial role in maintaining homeostasis, and microbial imbalances contribute to diseases such as periodontitis. Probiotic strains such as <em>Lactobacillus rhamnosus</em> and <em>Lactobacillus reuteri</em> have shown potential in restoring microbial balance in the oral cavity. However, their application remains challenging due to limited survival and adherence under intraoral conditions. Thus, we aimed to develop and evaluate mucoadhesive polymer films for local probiotic delivery. <em>L. rhamnosus</em> and <em>L. reuteri</em> were microencapsulated via spray drying and embedded in films composed of hydroxypropyl methylcellulose-polyvinyl alcohol (HPMC–PVA) and foamed polyvinyl alcohol (PVA). The films were characterized in terms of bacterial viability, tensile strength, folding endurance, and mucoadhesive properties. A proof-of-concept <em>in vivo</em> study was conducted by intraorally exposing enamel samples to two volunteers for eight hours, followed by confocal imaging and morphological analysis of adherent bacteria. Microencapsulation preserved high bacterial viability. The resulting films exhibited suitable mechanical properties and strong mucoadhesion. Biological evaluation revealed clear effects: films containing microencapsulated bacteria led to a statistically significant increase in adherent rod-shaped lactobacilli and a consistent reduction in coccoid bacteria associated with dysbiosis. The foamed PVA formulation showed the most pronounced modulation of the enamel-associated microbiota. These findings demonstrate that probiotic films can enable both bacterial stabilization and effective oral delivery. The system enhances colonization by beneficial bacteria while reducing potentially pathogenic cocci. This approach presents a promising strategy for microbiome-based prevention of oral diseases and merits further clinical investigation.</div></div>","PeriodicalId":279,"journal":{"name":"Colloids and Surfaces B: Biointerfaces","volume":"255 ","pages":"Article 114886"},"PeriodicalIF":5.4,"publicationDate":"2025-06-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144335786","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Alleviation of neuroinflammation on electrode interface by biomimetic electrical microenvironment modulation based on collagen/polypyrrole composite film","authors":"Chengwei Wu ,&nbsp;Xiyue Duan ,&nbsp;Xuzhao He ,&nbsp;Wenjian Weng ,&nbsp;Hang Zhou ,&nbsp;Gaorong Han ,&nbsp;Zuobing Chen ,&nbsp;Wen Chen ,&nbsp;Kui Cheng","doi":"10.1016/j.colsurfb.2025.114885","DOIUrl":"10.1016/j.colsurfb.2025.114885","url":null,"abstract":"<div><div>Neuroinflammation associated with inflammatory activation of astrocytes on the interface of implanted neural electrodes is the main cause of electrode failure. In this work, collagen/polypyrrole composite film (CPF) was designed and fabricated to modify the interface. The CPF had the co-exposure of collagen and polypyrrole in morphology, which allowed the biomimetic microenvironment provided by collagen to effectively inhibit the inflammatory activation of astrocytes. Meanwhile, the polypyrrole in the composite film significantly improved the electrochemical performance of the electrode interface, enabling it to provide an electrical microenvironment that inhibited inflammatory activation of astrocytes under electrical stimulation. Moreover, mechanistic analysis showed that appropriate electrical stimulation could upregulate calcium ion concentration while inhibit the activation of SOCE and inflammation-related signaling pathways. However, over-voltage stimulation increased ROS levels and reactivated pro-inflammatory signaling pathways. In general, benefiting from having both collagen biochemical cues and the ability to regulate the electrical microenvironment, CPF enabled astrocytes to have the lowest inflammatory factor secretion and inflammatory marker expression under appropriate electrical stimulation, effectively inhibiting the exacerbation of neuroinflammation. This work provides a new perspective on anti-neuroinflammation of the electrode interfaces.</div></div>","PeriodicalId":279,"journal":{"name":"Colloids and Surfaces B: Biointerfaces","volume":"255 ","pages":"Article 114885"},"PeriodicalIF":5.4,"publicationDate":"2025-06-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144330984","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Ternary composite material based on copper-metal organic frameworks, Mxene and carbon nanotubes as a sensitive electrochemical platform for determination of dopamine in aquatic environments","authors":"Ziyu Yuan ,&nbsp;Yao Zhu ,&nbsp;Haiyan Wu ,&nbsp;Fei Wang ,&nbsp;Yuhao Yin ,&nbsp;Long Qian ,&nbsp;Yuting Dai ,&nbsp;Tao Zhang ,&nbsp;Donya Yang ,&nbsp;Longbao Yu ,&nbsp;Fengxian Qiu","doi":"10.1016/j.colsurfb.2025.114882","DOIUrl":"10.1016/j.colsurfb.2025.114882","url":null,"abstract":"<div><div>Dopamine (DA) is a crucial neurotransmitter, however, its excessive use can lead to its accumulation as a micropollutant in aquatic ecosystems, thereby presenting various health risks to humans. Consequently, the accurate determination of dopamine levels is of paramount importance and has attracted considerable attention within scientific and regulatory communities. In this work, a ternary composite material (Cu-MOF/MXene/CNTs, MMC) based on copper-metal organic framework (Cu-MOF), the MXene and carbon nanotubes (CNTs) was developed using the hydrothermal method for sensitive electrochemical detection of DA. The prepared MMC possessed a stable structure derived from Cu-MOF, an extensive surface area attributed to MXenes, and numerous conductive channels formed by CNTs. Consequently, the modified MMC demonstrated high performance in DA detection. The electrochemical sensing capabilities of the MMC were assessed utilizing cyclic voltammetry (CV), and differential pulse voltammetry (DPV) methodologies. Under the optimal conditions, a strong linear relationship was observed between peak current and DA concentration within the range of 0.1–50 µM, achieving a detection limit (LOD) as low as 0.035 µM. The modified MMC sensor was utilized to quantify trace concentrations of DA in actual water samples, with recovery rates ranging from 97.3 % to 103 %, indicating satisfactory performance. Experiments were conducted to demonstrate that this composite material exhibited significant selectivity for metal ions and met related objectives. It was also shown acceptable stability over a period of 30 days and a commendable reproducibility, with a relative standard deviation (RSD) of 0.3 % across different production batches. Moreover, a comprehensive investigation was undertaken into the kinetics and underlying mechanisms of the electrochemical reaction. The transfer of electrons and hydrogen ions facilitated the transformation of DA into dopaminoquinones (DQ), a process that was accelerated by the increased availability of active sites in the designed sensor. This work introduces a method for the precise detection of DA utilizing the active sites and conductive pathways offered by the synergistic effect of MOF, CNTs and MXene, which have excellent performance in both laboratory and real environment, indicating that the proposed sensor is highly effective for detecting DA in water samples.</div></div>","PeriodicalId":279,"journal":{"name":"Colloids and Surfaces B: Biointerfaces","volume":"254 ","pages":"Article 114882"},"PeriodicalIF":5.4,"publicationDate":"2025-06-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144306448","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Oral formulations for cannabidiol: Improved absolute oral bioavailability of biodegradable cannabidiol self-emulsifying drug delivery systems","authors":"Matthias Sandmeier ,&nbsp;Ee Tsin Wong ,&nbsp;Gitte Nykjær Nikolajsen ,&nbsp;Asef Purwanti ,&nbsp;Sera Lindner ,&nbsp;Andreas Bernkop-Schnürch ,&nbsp;Wenhao Xia ,&nbsp;Julia Hoeng ,&nbsp;Kathrine Kjær ,&nbsp;Heidi Ziegler Bruun ,&nbsp;Sanne Skov Jensen","doi":"10.1016/j.colsurfb.2025.114879","DOIUrl":"10.1016/j.colsurfb.2025.114879","url":null,"abstract":"<div><h3>Aim</h3><div>This study aimed to fabricate and evaluate three different SEDDS formulations to improve the oral bioavailability of cannabidiol (CBD). CBD has limited oral bioavailability due to its lipophilicity and extensive first-pass metabolism. By using different emulsifiers in the SEDDS formulations, this study seeks to optimize CBD loading capacities and enhance overall <em>in vivo</em> pharmacokinetic (PK) performance of CBD when administered orally.</div></div><div><h3>Methods</h3><div>SEDDS were developed using three types of emulsifiers: 1) PEG-free polyglycerol (PG)-based, 2) mixed zwitterionic phosphatidylcholine/short-chain PEG-based, and 3) long-chain PEG-based. The SEDDS formulations were characterized in vitro for surface properties, lipolysis, and mucus permeation, and their pharmacokinetic profiles were compared with Epidiolex, a marketed CBD formulation.</div></div><div><h3>Results</h3><div>SEDDS with increased payloads (20 % w/w) were successfully developed. These formulations rapidly emulsified upon contact with aqueous media, forming monodisperse droplets that retained high levels of CBD (92.95–93.54 %) within the lipid core. Mucus permeability studies revealed that steric and surface-specific parameters, such as hydrophobicity and zeta potential, led to increased permeability for PG-based SEDDS, while PEG-based SEDDS had significantly reduced permeation. Furthermore, PG-based formulations exhibited increased fatty acid release upon SEDDS degradation with both lipase and pancreatin compared to PEG-based formulations. The plasma CBD concentration following oral administration of the developed SEDDS suggested higher absolute bioavailability of the PG-based formulation (3.8 %) compared with Epidiolex (3.4 %). Additionally, the maximum plasma concentrations for the three developed SEDDS ranged from 30.6 to 35.8 ng/mL, surpassing that of Epidiolex (25.0 ng/mL).</div></div><div><h3>Conclusion</h3><div>These findings underscore the potential of SEDDS as an effective oral delivery system for CBD, capable of achieving higher CBD plasma concentrations than Epidiolex. Additionally, the biodegradable PG-based SEDDS demonstrated improved absolute bioavailability compared to Epidiolex, emphasizing the importance of formulation design in optimizing oral drug delivery systems.</div></div>","PeriodicalId":279,"journal":{"name":"Colloids and Surfaces B: Biointerfaces","volume":"255 ","pages":"Article 114879"},"PeriodicalIF":5.4,"publicationDate":"2025-06-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144313017","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Nanozymes for regulation of reactive oxygen species in biomedical applications: Design, enzyme-like activity and therapeutic mechanisms","authors":"Min Wang,&nbsp;Wenjia Xu,&nbsp;Yucui Ding,&nbsp;Xinyu Liu,&nbsp;Jianlong Fu,&nbsp;Peng Zhang","doi":"10.1016/j.colsurfb.2025.114874","DOIUrl":"10.1016/j.colsurfb.2025.114874","url":null,"abstract":"<div><div>Nanozymes, which are artificially synthesized nanomaterials exhibiting enzyme-like activity, have garnered significant attention in recent years due to their potential as viable substitutes for natural enzymes. They demonstrate similar enzymatic kinetic properties comparable to natural enzymes and offer several advantages, including enhanced stability, cost-effective storage and preparation, reusability, and scalability. These advantages make nanozymes particularly opportunities for various applications. Notably, nanozymes can effectively regulate reactive oxygen species (ROS), thereby helping to maintain dynamic redox equilibrium within biological systems, making those nanozymes suitable for ROS-related diseases treatment, such as inflammation, neurodegenerative disorders, and cardiovascular diseases. On the other side, some nanozymes can be employed to target and eliminate tumor cells or pathogens through ROS generation, thus presenting opportunities for such disease treatment. Recent achievements in the development of nanozymes within the pharmaceutical field have significantly expanded their potential for biomedical applications. This review provides a brief overview of the classification of nanozymes, enzyme-like activity and catalytic mechanisms, and applications in disease treatment. Additionally, the challenges and future prospects of nanozymes in the biological field are also discussed. This review aims to serve as a valuable resource for researchers across multiple biomedical fields, offering insights into the design and application of nanozymes in biomedical applications.</div></div>","PeriodicalId":279,"journal":{"name":"Colloids and Surfaces B: Biointerfaces","volume":"254 ","pages":"Article 114874"},"PeriodicalIF":5.4,"publicationDate":"2025-06-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144298773","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Sprayable ciprofloxacin loaded chitosan nanospheres-based topical formulation for eliminating multidrug-resistant bacteria and accelerating infected wound healing","authors":"Unqa Mustafa ,&nbsp;Yasra Sarwar ,&nbsp;Ameena Mobeen ,&nbsp;Haji Muhammad Shoaib Khan ,&nbsp;Waseem Abbas ,&nbsp;Maryam Iftikhar ,&nbsp;Yumna Zaheer ,&nbsp;Syeda Zunaira Bukhari ,&nbsp;Danish Hussain ,&nbsp;Ruibing An ,&nbsp;Jian Dong ,&nbsp;Ayesha Ihsan ,&nbsp;Muhammad Rizwan Younis","doi":"10.1016/j.colsurfb.2025.114880","DOIUrl":"10.1016/j.colsurfb.2025.114880","url":null,"abstract":"<div><div>Multidrug-resistant (MDR) bacteria represent a formidable global health crisis with a substantial risk of prolonged infections due to the diminishing efficacy of traditional antibiotic therapies against these resilient pathogens. A dual strategy offering rapid inactivation of MDR bacteria and reduction in wound area could effectively promote wound healing. In this work, we developed a sprayable topical formulation by integrating a biocompatible biopolymer chitosan with a chemotherapeutic drug ciprofloxacin. The as-developed ciprofloxacin-loaded chitosan nanospheres (CIP-CNSs) exhibited a high encapsulation efficiency (70 %) and robust <em>in vitro</em> antibacterial activity against different MDR strains such as <em>E. coli</em>, <em>S. aureus</em>, and <em>P. aeruginosa</em>. However, CIP-CNSs did not induce any adverse side effects either <em>in vitro</em> or topically on the skin as confirmed by cytotoxicity assay and acute dermal irritation test. Moreover, in albino rabbits wound model, CIP-CNSs significantly accelerated wound healing with a wound closure rates of 75–85 %, within the first-week of post-incision, as compared to a commercial ointment. Furthermore, histopathological staining confirmed faster re-epithelialization and collagen deposition in wounded regions, without any hemorrhage or tissue infection. This study underscores the potential of CIP-CNSs topical formulation as a viable therapeutic strategy against the burgeoning threat of MDR-associated wound infections.</div></div>","PeriodicalId":279,"journal":{"name":"Colloids and Surfaces B: Biointerfaces","volume":"254 ","pages":"Article 114880"},"PeriodicalIF":5.4,"publicationDate":"2025-06-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144298772","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Hydrogel loaded with mesalazine for local injected treatment of periodontitis with superior anti-inflammatory, mucosal healing and bone repair performance","authors":"Haoyu Wang ,&nbsp;Yuqi Wang ,&nbsp;Jingzheng Yi ,&nbsp;YuXin Bai ,&nbsp;Ying Zhang ,&nbsp;Min Hu ,&nbsp;Jiaqing Yan","doi":"10.1016/j.colsurfb.2025.114881","DOIUrl":"10.1016/j.colsurfb.2025.114881","url":null,"abstract":"<div><div>Periodontitis is a chronic inflammatory disease caused by the interaction between the plaque microorganisms in the periodontal tissues and host's immune system. There is a close association between inflammatory bowel disease (IBD) and periodontitis. The pathogenesis of both diseases is associated with the dysregulation of the local microbiota and immune system. Mesalazine (MSZ) is routinely used in the clinical treatment of IBD, and it exhibits excellent antioxidant and anti-inflammatory effects as a non-steroidal anti-inflammatory drugs (NSAIDs). Considering the established close association between IBD and periodontitis, we hypothesise that MSZ may exert a similarly positive effect in the treatment of periodontitis. Given that the erosion of saliva and gingival crevicular fluid can lead to drug loss, we designed a temperature sensitive hydrogel composed of chitosan (CS), sodium β-glycerophosphate (β-GP), and gelatin (gel) to enhance drug utilization. The released MSZ can suppress the synthesis and release of inflammatory cytokines IL-6, TNF-α and PGE2 in macrophages by inhibiting the phosphorylation of PI3K and AKT. In <em>in vivo</em> experiments, the hydrogel reduced the infiltration of inflammatory cells in periodontal tissues of rats with periodontitis, promoted the formation of a new junctional epithelium, facilitated the epithelial healing of periodontal pocket linings, and inhibited the resorption of alveolar bone. In this research, we compared the intestinal mucosa with periodontal pocket tissues to identify a novel approach for periodontitis treatment, thereby achieving repurposing of existing drugs. This not only expanded the application of NSAIDs in periodontitis treatment, but also provided insights for optimizing the treatment of other mucosal diseases.</div></div>","PeriodicalId":279,"journal":{"name":"Colloids and Surfaces B: Biointerfaces","volume":"255 ","pages":"Article 114881"},"PeriodicalIF":5.4,"publicationDate":"2025-06-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144335872","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Dynamic covalent bonding based polysaccharide hydrogels and their applications in wound dressings: A review","authors":"Haibing Gao ,&nbsp;Zhen Wang ,&nbsp;Ting Lin ,&nbsp;Guolong Zhang ,&nbsp;Xiao Han ,&nbsp;Cui Cheng","doi":"10.1016/j.colsurfb.2025.114878","DOIUrl":"10.1016/j.colsurfb.2025.114878","url":null,"abstract":"<div><div>Polysaccharide hydrogels have emerged as pivotal wound dressings due to their inherent biocompatibility and extracellular matrix-mimicking properties. However, conventional systems employing permanent covalent or physical crosslinks suffer from irreversibility, poor self-repair capacity, and limited responsiveness that compromise clinical efficacy. To address these challenges, dynamic covalent bonding strategies have been innovatively integrated into polysaccharide networks, enabling unprecedented functionalities through reversible bond reconfiguration. These advanced hydrogels exhibit intelligent characteristics including self-healing, injectable adaptability, and stimulus-responsive drug release, achieved through precisely engineered imine, borate ester, disulfide, and other dynamic linkages, which allows the polysaccharide hydrogels to better adapt to different shapes of wounds and self-repair after damage to extend the life of the dressing. In this review, we summarize different types of dynamic covalently cross-linked polysaccharide hydrogels and their applications as wound dressings in wound repair, discuss the prospects of polysaccharide hydrogels based on dynamic covalent bonding, and address some of the associated limitations.</div></div>","PeriodicalId":279,"journal":{"name":"Colloids and Surfaces B: Biointerfaces","volume":"254 ","pages":"Article 114878"},"PeriodicalIF":5.4,"publicationDate":"2025-06-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144279654","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Recent advances in the clinical application of transferosomes for skin cancer management","authors":"Mohaddish ,&nbsp;Md Jilani ,&nbsp;Urushi Rehman ,&nbsp;Ramasubbamma Ramaiah ,&nbsp;Umme Hani ,&nbsp;Garima Gupta ,&nbsp;Khang Wen Goh ,&nbsp;Prashant Kesharwani","doi":"10.1016/j.colsurfb.2025.114877","DOIUrl":"10.1016/j.colsurfb.2025.114877","url":null,"abstract":"<div><div>Skin cancer, encompassing melanoma and non-melanoma types, represents a significant health burden, with melanoma being particularly aggressive. Chemotherapy remains a cornerstone of treatment, integrating small molecule drugs, phytoceuticals, and large biomolecules to combat this malignancy. Topical drug delivery provides a non-invasive alternative; however, the skin's inherent barriers limit drug penetration, posing a significant therapeutic challenge. Transferosomes, specialized vesicular systems, have shown potential in overcoming these limitations. Incorporated into gels, these vesicles enhance skin permeation and extend drug contact duration, reducing the frequency of applications and associated side effects. The optimization of their composition—focusing on phospholipid selection, edge activators, and manufacturing techniques—is pivotal for achieving better drug retention, enhanced penetration, and control over particle size. This review delves into the overview of skin cancer, existing treatment modalities, and their inherent constraints. It emphasizes the advantages of topical drug delivery, examining the mechanisms by which transferosomes surmount delivery challenges. Additionally, it evaluates the application of transferosomes for small-molecule drugs and phytoceuticals, underscoring their role in advancing skin cancer therapy and enhancing treatment outcomes.</div></div>","PeriodicalId":279,"journal":{"name":"Colloids and Surfaces B: Biointerfaces","volume":"254 ","pages":"Article 114877"},"PeriodicalIF":5.4,"publicationDate":"2025-06-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144271740","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Ultrasound-responsive MoS2@pCu-MOF heterojunction scaffold: Enhancing bacterial membrane permeability, exhibiting efficient antibacterial activity and facilitating bone regeneration","authors":"Gao Pan ,&nbsp;Zheng Wang ,&nbsp;Qi Zhong ,&nbsp;Huixing Li ,&nbsp;Shuping Peng ,&nbsp;Cijun Shuai","doi":"10.1016/j.colsurfb.2025.114872","DOIUrl":"10.1016/j.colsurfb.2025.114872","url":null,"abstract":"<div><div>Phosphate-based Cu-MOF (<em>p</em>Cu-MOF), a bioactive material with copper ions as framework centers and phosphate as the skeleton, shows chemodynamic antibacterial properties and bone regeneration potential via <em>PO₄³ ⁻</em> release. However, its chemodynamic therapy (CDT) relies too much on cellular microenvironment's hydrogen peroxide (H₂O₂) level, and low-concentration reactive oxygen species (ROS) can't penetrate the bacterial membrane well, limiting antibacterial efficiency. To solve this, we created MoS₂@<em>p</em>Cu-MOF type-II heterojunction nanocomposites to boost bacterial membrane permeability, effectively eliminating bacteria and promoting bone regeneration. Sonodynamic therapy (SDT) increases membrane permeability, allowing ROS to penetrate and disrupt bacteria. The type-II heterojunction, with SDT ability, generates hydroxyl radicals (·OH) and singlet oxygen (¹O₂) independently of H₂O₂ level by promoting electron-hole pair separation, achieving high antibacterial activity. Also, heterojunction-induced microcurrents under SDT synergize with <em>PO₄³ ⁻</em> to stimulate osteogenesis. We incorporated MoS₂@pCu-MOF nanocomposites into a poly-l-lactic acid (PLLA) scaffold via selective laser sintering. Under ultrasonic stimulation, bacterial membrane permeability increases. Separated charge carriers bind to active sites of the MoS₂@<em>p</em>Cu-MOF heterojunction scaffold to generate ·OH and ¹O₂, enabling high antibacterial efficiency without relying on CDT. Electrochemical experiments showed improved electron-hole separation efficiency of the heterojunction scaffold. <em>PO₄³ ⁻</em> release and ultrasound-induced microcurrents promoted bone regeneration. Results indicated that the inhibition rates of <em>Staphylococcus aureus</em> (<em>S.aureus</em>) and <em>Escherichia coli</em> (<em>E.coli</em>) reached 99 % and 98.5 %, respectively. Notably, ultrasound-induced microcurrents enhanced the scaffold's osteogenic capacity by 36 % at the late osteogenesis stage on the basis of ion stimulation and facilitated the expression of osteogenic factors like Runx-2, Wnt-10b, and BMP-2. These findings suggest MoS₂@<em>p</em>Cu-MOF heterojunction scaffolds can achieve effective SDT/CDT dual-mode antibacterial activity and promote bone regeneration via ionic/sonoelectric dual-mode.</div></div>","PeriodicalId":279,"journal":{"name":"Colloids and Surfaces B: Biointerfaces","volume":"254 ","pages":"Article 114872"},"PeriodicalIF":5.4,"publicationDate":"2025-06-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144271623","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}