Colloids and Surfaces B: Biointerfaces最新文献

{"title":"Self-pumping Janus nanofiber membrane with pH monitoring capability, integrated with a drug-loaded fast-dissolving layer for enhanced chronic wound healing","authors":"Jingjing Xi ,&nbsp;Xiaoyan Qi ,&nbsp;Yuxin Zhao ,&nbsp;Yue Feng ,&nbsp;Zhaolei Peng ,&nbsp;Yulin Wang ,&nbsp;Chunyan Cai ,&nbsp;Dejun Yang ,&nbsp;Liping Chen ,&nbsp;Jia Luo ,&nbsp;Xiaofang Li","doi":"10.1016/j.colsurfb.2025.115096","DOIUrl":"10.1016/j.colsurfb.2025.115096","url":null,"abstract":"<div><div>The emergence of antimicrobial resistance poses significant challenges in conventional antibiotic treatments for chronic wound infections, highlighting an urgent need for alternative therapeutic strategies. To address this issue, we developed a multifunctional electrospun nanofiber dressing co-loaded with anthocyanin (ATH) and asiaticoside (AS) that possesses antimicrobial activity. The tri-layer dressing contains three functional components: a hydrophilic polyacrylonitrile-anthocyanin (PAN-ATH) layer for pH monitoring, a hydrophobic polycaprolactone (PCL) layer for exudate management, and a water-soluble pullulan-<em>Bletilla striata</em> polysaccharide-asiaticoside (PUL-BSP-AS) layer. Upon contact with wound exudate, the PUL-BSP-AS inner layer promptly dissolves to effectively suppress microbial growth during the early stage of wound healing. The exudate is then gradually absorbed through the hydrophobic PCL layer and transported to the hydrophilic PAN-ATH layer. The pH colorimetric analysis of Multilayer membrane established a precise exponential relationship between pH values and R/B as well as R/G ratios in the RGB color space. The Multilayer dressing can function as a biosensor to monitor wound pH levels and guide subsequent treatment. Antimicrobial assays demonstrated the antimicrobial activity (&gt;88 %) of Multilayer membranes against Gram-positive (<em>Staphylococcus aureus</em>, <em>Bacillus cereus</em>), Gram-negative (<em>Pseudomonas aeruginosa</em>) bacteria, and the fungus (<em>Candida albicans</em>). Comprehensive <em>in vitro</em> and <em>in vivo</em> studies, combined with histopathological analysis, demonstrated that the dressing accelerates wound healing through multiple mechanisms, including antimicrobial, anti-inflammatory, and antioxidant effects, as well as upregulation of TGF-β and COL-1 expression to enhance collagen deposition. This innovative multilayer nanofiber system presents a promising therapeutic approach for chronic wound management with significant clinical potential.</div></div>","PeriodicalId":279,"journal":{"name":"Colloids and Surfaces B: Biointerfaces","volume":"257 ","pages":"Article 115096"},"PeriodicalIF":5.6,"publicationDate":"2025-09-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145004711","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Nanomedicine strategies for disulfidptosis activation in SLC7A11-high tumors","authors":"Zhanzheng Ye ,&nbsp;Yinsha Yao ,&nbsp;Yitianhe Xu ,&nbsp;Jinyao Ye ,&nbsp;Qing Yao ,&nbsp;Longfa Kou ,&nbsp;Ruijie Chen","doi":"10.1016/j.colsurfb.2025.115094","DOIUrl":"10.1016/j.colsurfb.2025.115094","url":null,"abstract":"<div><div>Disulfidptosis is a recently identified form of regulated cell death (RCD) characterized by aberrant disulfide bond accumulation and cytoskeletal collapse under conditions of redox imbalance. SLC7A11-overexpressing tumors are uniquely susceptible to this pathway due to their elevated cystine uptake and dependence on glucose-driven NADPH production for redox maintenance. These metabolic liabilities create therapeutic opportunities to selectively trigger disulfidptosis via pharmacologic or material-based interventions. In this review, we provide a brief overview of the molecular basis and metabolic vulnerabilities underlying disulfidptosis and highlight emerging nanomedicine strategies designed to activate this mechanism. Nanoparticle-based systems have been developed to modulate SLC7A11 activity, inhibit glucose or NADPH supply, or amplify intracellular disulfide stress. We categorize these platforms into three mechanistic classes and discuss their design principles and functional outcomes. By integrating redox biology with smart delivery technologies, nanomedicine offers a promising route to exploit disulfidptosis for cancer therapy, particularly in tumors resistant to conventional treatments.</div></div>","PeriodicalId":279,"journal":{"name":"Colloids and Surfaces B: Biointerfaces","volume":"257 ","pages":"Article 115094"},"PeriodicalIF":5.6,"publicationDate":"2025-09-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145004709","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"In-situ extrusion 3D printing with tea polyphenol crosslinking for Hyaluronic acid sodium salt -based composite hydrogel scaffolds","authors":"Reyihanguli Alimu ,&nbsp;Lanlan Dong ,&nbsp;Patiguli Aihemaiti ,&nbsp;Houfeng Jiang ,&nbsp;Wurikaixi Aiyiti ,&nbsp;Cijun Shuai","doi":"10.1016/j.colsurfb.2025.115095","DOIUrl":"10.1016/j.colsurfb.2025.115095","url":null,"abstract":"<div><div>High-performance hydrogel biomaterials hold considerable promise for advanced wound care. However, the suboptimal mechanical properties of conventional hydrogel materials limit their practical application. In this study, Hyaluronic acid sodium salt (HA), xanthan gum (XG), and N-acryloyl-glycinamide (NAGA) hydrogels with porous structures were successfully fabricated using in-situ extrusion 3D printing technology, and a functionalization strategy involving tea polyphenol (TP) immersion was proposed to enhance material properties through additional hydrogen bonding. Systematic optimization of TP concentration and immersion time led to significant improvements in the resulting hydrogel: tensile strain increased from 95 % to 266 %, tensile stress improved 6.8-fold, and compressive stress increased 10-fold, with an elastic modulus of 0.68 MPa and toughness of 1.2 MJ/m³ . Furthermore, TP-treated hydrogels exhibited excellent biocompatibility and strong antioxidant activity and antibacterial activity. This study provides a simple yet effective secondary functionalization approach for enhancing the performance of 3D-printed hydrogels, offering a promising approach for multifunctional hydrogel biomaterials with integrated mechanical strength and biocompatibility. These findings open new avenues for advanced wound care systems and personalized medicine applications.</div></div>","PeriodicalId":279,"journal":{"name":"Colloids and Surfaces B: Biointerfaces","volume":"257 ","pages":"Article 115095"},"PeriodicalIF":5.6,"publicationDate":"2025-09-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145010719","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Natural quercetin-derived carbon nanodots for dual antibacterial and anti-inflammatory therapy against bacterial infections","authors":"Mingna Hu ,&nbsp;Panyong Wang ,&nbsp;Juan Yue ,&nbsp;Huimin Miao ,&nbsp;Qiannan You ,&nbsp;Zixuan Jia ,&nbsp;Haiyang Yan ,&nbsp;Jinyu Yao ,&nbsp;Zhimin Chang ,&nbsp;Li Li","doi":"10.1016/j.colsurfb.2025.115097","DOIUrl":"10.1016/j.colsurfb.2025.115097","url":null,"abstract":"<div><div>Bacterial infections and the associated inflammatory responses present significant challenges to public health, underscoring the need for innovative therapeutic strategies. In this study, novel carbon dots (QA-CDs) derived from quercetin (QU) and 4-aminophenol (4-AP) were synthesized using a one-step hydrothermal method. This approach merges the antimicrobial properties of phenolic compounds with the multifunctional advantages of carbon-based nanomaterials. The resulting QA-CDs exhibited a spherical morphology with an average diameter of 3.23 nm and abundant surface functional groups. In vitro antimicrobial assays demonstrated that QA-CDs effectively inhibited the growth of <em>E. coli</em> and <em>S. aureus</em>, with minimum bactericidal concentrations (MBCs) of 100 μg/mL and 25 μg/mL, respectively. Mechanistic studies revealed that QA-CDs disrupted bacterial membrane integrity, induced nucleic acid leakage, and exhibited strong reactive oxygen species (ROS) scavenging activity. Notably, QA-CDs significantly downregulated the mRNA expression of pro-inflammatory cytokines (TNF-α, IL-6, IL-1β, iNOS, and TGF-β1) while upregulating the anti-inflammatory cytokine IL-10 in lipopolysaccharide (LPS)-induced RAW264.7 cells. In vivo studies using murine models of peritonitis and pneumonia showed that QA-CDs completely eradicated <em>MRSA</em> in infected tissues and reduced TNF-α and IL-1β levels in organ lavage fluids, confirming their dual antibacterial and anti-inflammatory effects. With excellent biocompatibility (hemolysis rate &lt;5 % and no significant organ toxicity) and broad-spectrum antimicrobial activity, QA-CDs represent a promising nanotherapeutic strategy for treating bacterial infections and associated inflammatory disorders.</div></div>","PeriodicalId":279,"journal":{"name":"Colloids and Surfaces B: Biointerfaces","volume":"257 ","pages":"Article 115097"},"PeriodicalIF":5.6,"publicationDate":"2025-09-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144933609","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Mechanisms of Interaction Between Bovine Serum Albumin and Novel Schiff Base Compounds: A Multi-Spectroscopic, DFT Analysis and In Silico Investigation","authors":"Gül Kotan ,&nbsp;Tuğba Bayraktutan ,&nbsp;Ömer Faruk Bayraktutan ,&nbsp;Haydar Yüksek","doi":"10.1016/j.colsurfb.2025.115080","DOIUrl":"10.1016/j.colsurfb.2025.115080","url":null,"abstract":"<div><div>The synthesis of two Schiff base (SB) compounds, 3-phenyl-4-(5-nitrofuran-1-yl)methyleneamino-4,5-dihydro-1<em>H</em>-1,2,4-triazol-5-one (3‒PNM) and 1-acetyl-3-phenyl-4-(5-nitrofuran-1-yl)methyleneamino-4,5-dihydro-1<em>H</em>-1,2,4-triazol-5-one (1-APNM) was successful. The structures of 3-PNM and 1-APNM were determined using ¹H/¹C-NMR and IR spectroscopy. Absorption and fluorescence spectroscopy were used to evaluate the compounds' interactions with BSA. The SB quenched BSA fluorescence, indicating a 1:1 complex was formed by SB-BSA complex static quenching. BSA and SB binding constants (Kb) and binding sites (n) were used to investigate binding kinetics. Three-dimensional and synchronized fluorescence spectroscopy examined how triazole pairings affect BSA's second structure. Also, theoretical computations of molecules were done. Here, Gaussview 5.0 and Gaussian 09 were utilized. Imported theoretical properties (FMOs, NLO, MEP, GRPs, ΔEg, mulliken population) of molecules were estimated using B3LYP functional and 6-311G (d,p) basis set of DFT. Both molecule altered BSA's structure, possibly impacting its physiological capability.</div></div>","PeriodicalId":279,"journal":{"name":"Colloids and Surfaces B: Biointerfaces","volume":"257 ","pages":"Article 115080"},"PeriodicalIF":5.6,"publicationDate":"2025-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145004707","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Mussel-inspired nanocellulose scaffold for antibacterial and anti-inflammatory coating for clear aligners","authors":"Xiaoyi Huang ,&nbsp;Wenbin Huang ,&nbsp;Junqin Wang ,&nbsp;Rui Mu ,&nbsp;Xueqin Bai ,&nbsp;Yuntao Lin ,&nbsp;Yuling Chen ,&nbsp;Xiaolian Li ,&nbsp;Feilong Jiang ,&nbsp;Ju Fang ,&nbsp;Fuzeng Ren ,&nbsp;Tao Pei","doi":"10.1016/j.colsurfb.2025.115091","DOIUrl":"10.1016/j.colsurfb.2025.115091","url":null,"abstract":"<div><div>Aesthetic demands in contemporary dentistry have driven the widespread adoption of clear aligners (CAs), yet their polymeric substrates lack intrinsic antibacterial and anti-inflammatory functionality, predisposing the periodontium to inflammation during prolonged wear. Here, we present a multifunctional surface coating for PETG-based CAs, comprising dopamine-grafted TEMPO-oxidized bacterial cellulose nanofibers (TOBC-DA), curcumin (Cur), and polyvinyl alcohol (PVA), applied via a facile spin-coating process. TOBC-DA serves as a bioadhesive matrix that forms a robust, long-lasting bond with PETG while maintaining chemical stability in simulated oral conditions. Incorporation of Cur endows the coating with potent antibacterial and anti-inflammatory activity, and PVA enhances Cur solubility to ensure uniform film formation. The resulting composite layer-measuring under 5 <em>μ</em>m in thickness-preserves the optical transparency, dimensional fidelity, and mechanical integrity of PETG. Structurally, the cross-linked TOBC-DA network and PVA backbone confer enhanced wear resistance and fracture resistance without compromising flexibility. Functionally, the hydrophilic surface repels early colonizers such as <em>P. gingivalis</em>, while sustained Cur release disrupts bacterial cell membranes and downregulates pro-inflammatory mediators (IL-6, TNF-α, MMP-9) in periodontal tissues. This work demonstrates a clinically translatable coating strategy that integrates aesthetic preservation with active periodontal protection, offering a promising route to improve oral health outcomes during CAs therapy.</div></div>","PeriodicalId":279,"journal":{"name":"Colloids and Surfaces B: Biointerfaces","volume":"257 ","pages":"Article 115091"},"PeriodicalIF":5.6,"publicationDate":"2025-08-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144933675","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Injectable biomimetic nano-hydrogel composite based on copper selenide nanoparticles and carboxymethyl chitosan for synergistic chemo-photothermal cancer therapy","authors":"Yuanpeng Wang ,&nbsp;Yuping Yang ,&nbsp;Jiachi Ma ,&nbsp;Juanjuan Wang ,&nbsp;Liang Chu ,&nbsp;Qingkang Wang ,&nbsp;Siyu Sun ,&nbsp;Xin Wang ,&nbsp;Chensong Zhang","doi":"10.1016/j.colsurfb.2025.115092","DOIUrl":"10.1016/j.colsurfb.2025.115092","url":null,"abstract":"<div><div>This study reports the synthesis and characterization of an injectable nano-hydrogel composite (m@NPs-HG) based on selenium nanoparticles (Se NPs) and carboxymethyl chitosan (CMCS) nanoparticles for enhanced cancer therapy. Selenium nanoparticles were stabilized using CMCS to form copper selenide nanoparticles (CSe NPs), while doxorubicin (DOX)-loaded CMCS nanoparticles (CD NPs) were encapsulated within cancer cell membranes to generate biomimetic nanoparticles (m@NPs). Subsequently, CSe NPs and m@NPs were integrated into a hydrogel via crosslinking with CuCl₂, resulting in the formation of m@NPs-HG. The composite exhibited remarkable photothermal conversion capability, efficient cellular uptake, and robust reactive oxygen species (ROS) generation. <em>In vitro</em> experiments demonstrated significant induction of apoptosis and cytotoxicity in H22 and HepG2 cancer cells. The <em>in vivo</em> anti-tumor efficacy was evaluated in H22 tumor-bearing mice, revealing that m@NPs-HG combined with laser irradiation effectively suppressed tumor growth while exhibiting minimal systemic toxicity. Hemolysis and biodistribution studies further confirmed the excellent biocompatibility and targeting ability of the composite system. This study concludes that the m@NPs-HG system represents a promising theranostic platform for cancer treatment.</div></div>","PeriodicalId":279,"journal":{"name":"Colloids and Surfaces B: Biointerfaces","volume":"256 ","pages":"Article 115092"},"PeriodicalIF":5.6,"publicationDate":"2025-08-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144923003","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Reactive oxygen species-responsive virus-mimicking nanodrug for the treatment of influenza A virus infection","authors":"Hong-Li Wang ,&nbsp;ChuanMing Yu ,&nbsp;Qiangling Yin ,&nbsp;Jin-Wei Bu ,&nbsp;Shuang Feng ,&nbsp;Di Ning ,&nbsp;Shu-Lin Liu ,&nbsp;Linlin Liu ,&nbsp;Zhi-Gang Wang","doi":"10.1016/j.colsurfb.2025.115090","DOIUrl":"10.1016/j.colsurfb.2025.115090","url":null,"abstract":"<div><div>Drug therapy is an important measure to reduce the morbidity and mortality of influenza. However, small molecule drugs have inherent limitations, such as poor water solubility, non-specific biological distribution, and susceptibility to degradation during blood circulation, which impose a great burden on patients, both physically and mentally. Inspired by the high levels of reactive oxygen species (ROS) at the site of influenza A virus (IAV) infection, we have developed an intelligent responsive virus-mimicking nanodrug (Zana@HA-Lip) based on a biomimetic approach, reduces the damage of the drug to normal tissues or organs while achieving the purpose of antiviral therapy by precisely releasing the drug at the lesion site. Zana@HA-Lip offers distinct advantages regarding lesion site tropism, duration of action, and efficiency of release in response to ROS. This work not only presents a safe and effective strategy for alleviating lung injury caused by IAV infection but also introduces a new concept for the precise customization of virus-mimicking nanocarriers.</div></div>","PeriodicalId":279,"journal":{"name":"Colloids and Surfaces B: Biointerfaces","volume":"257 ","pages":"Article 115090"},"PeriodicalIF":5.6,"publicationDate":"2025-08-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144933611","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Hierarchical materials: An overview of synthesis methods and revolutionary impact on enzyme biocatalysis","authors":"Elizabet Moreno-Reyes,&nbsp;Julie M. Goddard","doi":"10.1016/j.colsurfb.2025.115086","DOIUrl":"10.1016/j.colsurfb.2025.115086","url":null,"abstract":"<div><div>Enzymes are exceptional biological catalysts given their high substrate specificity and ability to accelerate chemical reactions under mild conditions. To harness the potential of enzymes outside their native environments, immobilization technologies continue to emerge enhancing enzyme stability and handling. Insoluble materials have been widely used as enzyme supports, and their composition and structure usually determine the performance of the resulting biocatalysts. Although numerous studies have been reported on materials and techniques for enzyme immobilization, little attention has been given to hierarchical materials in enzyme stabilization and bioprocess intensification. Thus, the purpose of this review is to survey the technological potential of hierarchical materials in enzyme immobilization and bioprocessing. Herein, we describe reported synthesis methods to prepare hierarchical materials and discuss how these materials have been exploited in biocatalyst designs, their influence on the activity of enzymes post-immobilization, and the benefits they offer to large scale bioprocessing. We conclude with insights into industrially translatable hierarchical material synthesis methods and biocatalyst designs as well as aspects of research that need attention to continue advancing enzyme immobilization technologies in biocatalysis.</div></div>","PeriodicalId":279,"journal":{"name":"Colloids and Surfaces B: Biointerfaces","volume":"256 ","pages":"Article 115086"},"PeriodicalIF":5.6,"publicationDate":"2025-08-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144916959","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Injectable chitosan-based thermosensitive hydrogel loaded with adipose-derived mesenchymal stem cells promotes pressure ulcer healing","authors":"Simeng He ,&nbsp;Bin Lin ,&nbsp;Chenhong Zhang ,&nbsp;Shuzhen He ,&nbsp;Yunquan Zheng ,&nbsp;Xianai Shi ,&nbsp;Jianmin Yang","doi":"10.1016/j.colsurfb.2025.115089","DOIUrl":"10.1016/j.colsurfb.2025.115089","url":null,"abstract":"<div><div>Pressure ulcers, resulting from prolonged external pressure or shear forces on skin and underlying tissues over bony prominences, lead to tissue ischemia and impaired lymphatic drainage. Without timely intervention, these wounds can progress to severe complications including cellulitis, chronic infections, and osteomyelitis. In this study, we developed a chitosan/sodium β-glycerophosphate/gelatin (CS/β-GP/GEL) thermosensitive hydrogel system to enhance the therapeutic efficacy of adipose-derived mesenchymal stem cells (ADSCs) in pressure ulcer healing. The incorporation of gelatin addresses the limitations of conventional CS/β-GP hydrogels, such as low mechanical strength and poor biocompatibility. The optimized CS/β-GP/GEL hydrogel exhibits good injectability, suitable gel formation time and pH, facilitating efficient ADSCs encapsulation. Furthermore, the hydrogel demonstrates good water absorption capacity, degradability, and rheological properties, making it suitable for biomedical applications. <em>In vitro</em> studies confirmed the hydrogel's high cytocompatibility, supporting ADSCs viability and proliferation. Additionally, the CS/β-GP/GEL@ADSC composite demonstrated pro-angiogenic properties, suppressed reactive oxygen species-mediated apoptosis, and facilitated the accumulation of M2-type macrophages. <em>In vivo</em> evaluations revealed that the CS/β-GP/GEL@ADSC composite had high histocompatibility and accelerated pressure ulcer healing in a rat model, mediated through enhanced angiogenesis, M2-dominant macrophage polarization, and improved extracellular matrix remodeling. These findings highlight the potential of CS/β-GP/GEL@ADSC as a promising therapeutic strategy for pressure ulcer management.</div></div>","PeriodicalId":279,"journal":{"name":"Colloids and Surfaces B: Biointerfaces","volume":"256 ","pages":"Article 115089"},"PeriodicalIF":5.6,"publicationDate":"2025-08-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144919697","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}