Colloids and Surfaces B: Biointerfaces最新文献_第10页

Advances in coagulation peptides: Exploring diverse functions from hemostasis to disease treatment 凝血肽的研究进展：探索从止血到疾病治疗的多种功能

IF 5.6 2区医学

Colloids and Surfaces B: Biointerfaces Pub Date : 2026-04-01 Epub Date: 2025-12-23 DOI: 10.1016/j.colsurfb.2025.115383

Wenwen Ma , Yumei Wang , Xin Liu , Wen Li , Wenxue Zhao , Jumeng Di , Hailin Cong , Bing Yu

{"title":"Advances in coagulation peptides: Exploring diverse functions from hemostasis to disease treatment","authors":"Wenwen Ma , Yumei Wang , Xin Liu , Wen Li , Wenxue Zhao , Jumeng Di , Hailin Cong , Bing Yu","doi":"10.1016/j.colsurfb.2025.115383","DOIUrl":"10.1016/j.colsurfb.2025.115383","url":null,"abstract":"<div><div>Coagulation dysfunction and bleeding control remain major challenges in trauma, surgery, and interventional therapy. Millions of patients die from excessive blood loss every year. Traditional hemostatic materials such as gelatin sponge, chitosan, and zeolite have limitations such as a single mechanism of action and poor biological stability. At the same time, clotting peptides have attracted much attention due to their low molecular weight, high biological activity, and precisely designed molecular structure. This paper systematically reviews the latest progress of coagulation peptides as a new hemostatic material, discusses their interaction mechanism with coagulation factors and platelet receptors, and analyzes the structural design strategy. Subsequently, this article critically analyzed the clinical trial data, efficacy comparison, and long-term safety evaluation of clotting peptides in the fields of gastrointestinal endoscopy, high-risk surgery, and systemic hemostasis. Finally, this article summarizes the key clinical translational barriers faced by the study of clotting peptides, including challenges such as long-term biocompatibility, potential thrombosis risks, and in vivo degradation characteristics. This review aims to provide systematic theoretical guidance and objective clinical transformation analysis for the rational design and performance optimization of a new generation of multifunctional coagulation peptide materials.</div></div>","PeriodicalId":279,"journal":{"name":"Colloids and Surfaces B: Biointerfaces","volume":"260 ","pages":"Article 115383"},"PeriodicalIF":5.6,"publicationDate":"2026-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145838041","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Microrobots/micromotors in cancer therapeutics, advances and applications 微型机器人/微型马达在癌症治疗中的进展和应用。

IF 5.6 2区医学

Colloids and Surfaces B: Biointerfaces Pub Date : 2026-04-01 Epub Date: 2025-12-23 DOI: 10.1016/j.colsurfb.2025.115394

Hezhe Huang, Yuqing Miao, Yuhao Li

{"title":"Microrobots/micromotors in cancer therapeutics, advances and applications","authors":"Hezhe Huang, Yuqing Miao, Yuhao Li","doi":"10.1016/j.colsurfb.2025.115394","DOIUrl":"10.1016/j.colsurfb.2025.115394","url":null,"abstract":"<div><div>Cancer remains one of the most challenging diseases to conquer due to its high mortality rate and the lack of effective diagnostic and therapeutic tools. Microrobot technology has brought revolutionary advancements to the field of tumor medicine. By leveraging its micro/nanoscale dimensions and precise propulsion and navigation capabilities, it has enhanced the accuracy and efficiency of cancer diagnosis and treatment. This review provides a detailed exploration of microrobots’ motion from the perspectives of propulsion and navigation. It systematically summarizes its key applications in cancer therapy. Microrobots, which utilize gas, magnetic, light, or ultrasound-based propulsion mechanisms, and are combined with asymmetrical morphologies or biotargeted navigation, can traverse complex biological barriers such as the blood-brain barrier and mucus barrier. They enable precise delivery and controlled release of anticancer drugs. In the realm of diagnosis and imaging, microrobots, by carrying fluorescent probes or contrast agents, have improved the sensitivity of tumor detection and the resolution of deep tissue imaging. Furthermore, microrobots, when coordinated with photothermal therapy, sonodynamic therapy, or immunotherapy, have enhanced tumor eradication effects. They also activate immune responses to inhibit metastatic lesions. Finally, this review provides an outlook on the prospects of microrobots in tumor medicine, proposing potential collaborative pathways with emerging technologies. It highlights their vast potential in improving therapeutic efficacy and reducing side effects.</div></div>","PeriodicalId":279,"journal":{"name":"Colloids and Surfaces B: Biointerfaces","volume":"260 ","pages":"Article 115394"},"PeriodicalIF":5.6,"publicationDate":"2026-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145861605","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Frankincense-loaded lactoferrin-conjugated solid lipid nanoparticles for targeted brain delivery and neuroprotection in a scopolamine-induced Alzheimer’s model 乳香负载的乳铁蛋白结合固体脂质纳米颗粒在东莨菪碱诱导的阿尔茨海默病模型中的靶向脑递送和神经保护

IF 5.6 2区医学

Colloids and Surfaces B: Biointerfaces Pub Date : 2026-04-01 Epub Date: 2025-12-13 DOI: 10.1016/j.colsurfb.2025.115370

Farimah Moazzam , Ashrafalsadat Hatamian-Zarmi , Marzie Sabaghian , Khadijeh Khezri , Bahman Ebrahimi Hosseinzadeh , Fariba Khodagholi , Moloud Jalili Shahmansouri , Fatemeh Sadat Rashidi

{"title":"Frankincense-loaded lactoferrin-conjugated solid lipid nanoparticles for targeted brain delivery and neuroprotection in a scopolamine-induced Alzheimer’s model","authors":"Farimah Moazzam , Ashrafalsadat Hatamian-Zarmi , Marzie Sabaghian , Khadijeh Khezri , Bahman Ebrahimi Hosseinzadeh , Fariba Khodagholi , Moloud Jalili Shahmansouri , Fatemeh Sadat Rashidi","doi":"10.1016/j.colsurfb.2025.115370","DOIUrl":"10.1016/j.colsurfb.2025.115370","url":null,"abstract":"<div><div>Central nervous system (CNS) disorders remain a major field with substantial unmet therapeutic needs. This study aimed to address the critical challenge of brain drug delivery by employing solid lipid nanoparticles (SLNs) surface-functionalized with lactoferrin (Lf) ligands, from the transferrin family, to facilitate transport across the blood–brain barrier. Frankincense (F), a natural compound with well-documented neuroprotective properties and minimal adverse effects, was encapsulated within (SLNs) using a microemulsion approach. The optimal formulation resulted in nanoparticles (NPs) with an average size of 103.1 ± 0.9 nm, an encapsulation efficiency of 95.2 ± 0.8 %, a drug loading capacity of 8.6 ± 0.1 %, a polydispersity index (PDI) of 0.3 ± 0.08, and a zeta potential of −29.2 ± 0.5 mV. The optimized NPs showed a sustained drug release profile, and ATR-FTIR spectra confirmed the conjugation of lactoferrin to the nanoparticles. To establish translational relevance, the neuroprotective effectiveness of (F-Lf-SLNs) was investigated using a scopolamine-induced Alzheimer's disease animal model. Behavioral tests, along with biochemical and histological analyses, were conducted. The findings demonstrated that (F-Lf-SLNs) significantly improved the brain delivery of frankincense, highlighting their promise as a neuroprotective strategy for CNS disorders. They also effectively protected neurons compared to the scopolamine-induced group. Overall, these results emphasize the key role of drug delivery systems and ligand-targeting methods in enhancing the therapeutic effectiveness of drugs.</div></div>","PeriodicalId":279,"journal":{"name":"Colloids and Surfaces B: Biointerfaces","volume":"260 ","pages":"Article 115370"},"PeriodicalIF":5.6,"publicationDate":"2026-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145754008","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The MCM/Lys-Cys nanodevices for the efficient gene delivery: An approach towards MCP1 gene manipulation using CRISPR technology 用于高效基因传递的MCM/Lys-Cys纳米器件：使用CRISPR技术操作smcp1基因的方法。

IF 5.6 2区医学

Colloids and Surfaces B: Biointerfaces Pub Date : 2026-04-01 Epub Date: 2025-12-16 DOI: 10.1016/j.colsurfb.2025.115377

Azadeh Rahimi , Ilnaz Rahimmanesh , Navid Abedpoor , Maryam Boshtam , Elham Bidram , Shaghayegh Haghjooy Javanmard , Hossein Khanahmad , Laleh Rafiee , Ashkan Bigham , Mohammad Rafienia , Saeed Karbasi , Laleh Shariati

{"title":"The MCM/Lys-Cys nanodevices for the efficient gene delivery: An approach towards MCP1 gene manipulation using CRISPR technology","authors":"Azadeh Rahimi , Ilnaz Rahimmanesh , Navid Abedpoor , Maryam Boshtam , Elham Bidram , Shaghayegh Haghjooy Javanmard , Hossein Khanahmad , Laleh Rafiee , Ashkan Bigham , Mohammad Rafienia , Saeed Karbasi , Laleh Shariati","doi":"10.1016/j.colsurfb.2025.115377","DOIUrl":"10.1016/j.colsurfb.2025.115377","url":null,"abstract":"<div><div>Breast cancer continues to be the most common malignancy among women worldwide, requiring novel therapeutic approaches. This research investigates an innovative gene delivery strategy employing mesoporous silica nanoparticles (MCM-41) modified with lysine and cysteine (Lys-Cys) for the effective delivery of CRISPR-Cas9 plasmids aimed at the monocyte chemoattractant protein-1 (MCP-1/CCL2) gene. Bioinformatics analysis of the TCGA-BRCA dataset revealed substantial deregulation of CCL2 in breast cancer, underscoring its involvement in tumor growth and inflammation. The MCM/Lys-Cys nanocarrier demonstrated remarkable biocompatibility and effectively encapsulated a plasmid containing GFP, promoting superior cellular uptake in MDA-MB-231 breast cancer cells compared to conventional techniques. Functional experiments demonstrated that CRISPR/Cas9-mediated suppression of CCL2 markedly decreased cell proliferation, migration, and invasion, highlighting the promise of this targeted gene therapy strategy in breast cancer management. The findings indicate that the MCM/Lys-Cys nanosystem presents a viable non-viral approach for precise gene editing, potentially boosting therapeutic efforts against breast cancer by modulating inflammatory pathways.</div></div>","PeriodicalId":279,"journal":{"name":"Colloids and Surfaces B: Biointerfaces","volume":"260 ","pages":"Article 115377"},"PeriodicalIF":5.6,"publicationDate":"2026-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145825404","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Baicalein-manganese nanoparticles for antioxidant and osteogenic therapy of bone injury 黄芩素-锰纳米颗粒抗氧化和成骨治疗骨损伤。

IF 5.6 2区医学

Colloids and Surfaces B: Biointerfaces Pub Date : 2026-04-01 Epub Date: 2025-12-26 DOI: 10.1016/j.colsurfb.2025.115400

Ruqi Wang , Qishu Jin , Zhaoxu Meng , Xiaodong Cao , Yihao Wu , Chen Yang , Jiandong Yuan

{"title":"Baicalein-manganese nanoparticles for antioxidant and osteogenic therapy of bone injury","authors":"Ruqi Wang , Qishu Jin , Zhaoxu Meng , Xiaodong Cao , Yihao Wu , Chen Yang , Jiandong Yuan","doi":"10.1016/j.colsurfb.2025.115400","DOIUrl":"10.1016/j.colsurfb.2025.115400","url":null,"abstract":"<div><div>Oxidative stress is increasingly recognized as a key pathological driver of skeletal degeneration in diabetes, aging, trauma, and other orthopedic disorders, where excessive reactive oxygen species (ROS) compromise osteoblast and bone marrow mesenchymal stem cell (BMSC) function while promoting osteoclast activity. Natural polyphenols such as baicalein (BA) exhibit potent antioxidant effects but are hindered by poor solubility and limited stability. Here, we report a series of BA-X self-assembled nanoparticles, in which X represents osteogenic metal ions including Mg<sup>2 +</sup> , Mn<sup>2+</sup>, Cu<sup>2+</sup>, Zn<sup>2+</sup>, and Sr<sup>2+</sup>. Among these, BA-Mn demonstrated the most robust radical-scavenging activity in DPPH, ABTS, and multiple ROS-targeted assays. BA-Mn exhibited nanoscale dimensions, uniform coordination, and excellent cytocompatibility, significantly reducing intracellular ROS and protecting H<sub>2</sub>O<sub>2</sub>-injured BMSCs. Functionally, BA-Mn restored osteogenic differentiation under oxidative stress, enhancing alkaline phosphatase activity, mineral deposition, and osteogenic gene expression. Transcriptomic analysis further revealed concurrent activation of cell cycle, PI3K-Akt, and MAPK signaling pathways, alongside suppression of ferroptosis and ROS-related carcinogenesis. Collectively, these findings establish BA-Mn as a multifunctional nanoplatform that integrates antioxidant defense with osteogenic stimulation, offering a promising therapeutic approach for oxidative stress-related bone disorders.</div></div>","PeriodicalId":279,"journal":{"name":"Colloids and Surfaces B: Biointerfaces","volume":"260 ","pages":"Article 115400"},"PeriodicalIF":5.6,"publicationDate":"2026-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145877313","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Surface modification of PET Fiber: Evaluation of the synergistic effect of a thermostable engineered cutinase with DBD plasma pretreatment PET纤维的表面改性：热稳定性工程角质酶与DBD血浆预处理协同作用的评价。

IF 5.6 2区医学

Colloids and Surfaces B: Biointerfaces Pub Date : 2026-04-01 Epub Date: 2025-12-30 DOI: 10.1016/j.colsurfb.2025.115408

Afsaneh Zarei , Farzaneh Alihosseini , Farzaneh Jafary

{"title":"Surface modification of PET Fiber: Evaluation of the synergistic effect of a thermostable engineered cutinase with DBD plasma pretreatment","authors":"Afsaneh Zarei , Farzaneh Alihosseini , Farzaneh Jafary","doi":"10.1016/j.colsurfb.2025.115408","DOIUrl":"10.1016/j.colsurfb.2025.115408","url":null,"abstract":"<div><div>Poly (ethylene terephthalate) (PET) is a widely used synthetic polymer in textiles, but its hydrophobicity limits its applications despite excellent mechanical properties. Enzymatic hydrolysis provides a sustainable and environmentally friendly approach for PET surface modification, but its efficiency is restricted by the polymer’s high crystallinity and limited enzyme accessibility. In this study, a synergistic plasma-enzyme treatment strategy was developed to enhance PET fiber surface hydrophilicity. Molecular dynamics (MD) simulations were employed to identify thermally unstable regions in leaf-branch compost cutinase (LCC), guiding the design of a thermostable variant, KI (T60K/N122I). The engineered KI exhibited a 10 °C improvement in thermal stability and a 1.33-fold increase in hydrolytic efficiency on amorphous PET compared to the native enzyme. PET fibers were subjected to ten sequential plasma–enzyme treatment cycles (90 min, 70 °C each), varying plasma exposure time (90 s and 180 s) and reactive gas type (O₃ or O₂). Surface analyses revealed that pretreatment with O₃ plasma significantly enhanced enzyme activity by promoting higher surface roughness and introducing more oxygen-containing functional groups. The combination of O₃ plasma and KI resulted in 1.5-fold higher water absorption and 1.2-fold higher color strength relative to O₂ plasma–treated samples. Gravimetric measurements confirmed greater mass loss for the O₃–KI system, attributable to enhanced thermal robustness and substrate affinity of the engineered enzyme. Overall, the cyclic plasma–enzyme process demonstrated superior performance compared to conventional modification methods, providing an efficient and sustainable route for functionalizing PET surfaces.</div></div>","PeriodicalId":279,"journal":{"name":"Colloids and Surfaces B: Biointerfaces","volume":"260 ","pages":"Article 115408"},"PeriodicalIF":5.6,"publicationDate":"2026-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145916292","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Oral environment targeting sutures for refractory oral wound 口腔环境靶向缝合治疗难治性口腔创面

IF 5.6 2区医学

Colloids and Surfaces B: Biointerfaces Pub Date : 2026-04-01 Epub Date: 2025-12-25 DOI: 10.1016/j.colsurfb.2025.115388

Shuze Wang , Jin Wang , Yue Wang , Xintong Ye , Liyun Zhang , Guangqi Yan , Xue Wang

{"title":"Oral environment targeting sutures for refractory oral wound","authors":"Shuze Wang , Jin Wang , Yue Wang , Xintong Ye , Liyun Zhang , Guangqi Yan , Xue Wang","doi":"10.1016/j.colsurfb.2025.115388","DOIUrl":"10.1016/j.colsurfb.2025.115388","url":null,"abstract":"<div><div>The use of sutures for stable wound closure in the humid, microbiologically complex oral environment is critical for mucosal healing. However, traditional absorbable sutures often loosen due to oral movements, leading to micro-gaps and accelerated microbial colonization, which disrupts healing. Inspired by spider silk's \"expansion-induced supercontraction\" in humidity, this study develops tea polyphenols self-contracting sutures to enhance mechanical reinforcement and repair efficiency. This next-generation degradable suture features a spider silk-inspired elastic network: polyvinyl alcohol (PVA) chains provide entropy-driven elastic recovery, while an ion-crosslinked network of sodium alginate and Ca²⁺ serves as a moisture-responsive unit. When dry, the network maintains pre-tension. Upon hydration, water molecules competitively coordinate with alginate and Ca²⁺, reducing crosslink density. This allows the PVA network to actively contract within four hours post-surgery, approximating wound edges, minimizing gaps, and reducing bacterial invasion. This next-generation degradable suture exhibits robust antibacterial activity, with 90.3 % inhibition against E. coli and 95.6 % against S. aureus. It also exerts significant biological regulation: compared to controls, it upregulates anti-inflammatory IL-10 expression by 2.41-fold, enhances oxidative stress scavenging capacity by 1.87-fold, and promotes angiogenesis. <em>In vivo</em> tests on difficult oral wounds show this next-generation degradable suture accelerates healing, reduces scarring, and improves tissue regeneration quality, especially in infected wounds. It holds broad potential for minimally invasive oral and maxillofacial surgeries and infection-prone wound repair.</div></div>","PeriodicalId":279,"journal":{"name":"Colloids and Surfaces B: Biointerfaces","volume":"260 ","pages":"Article 115388"},"PeriodicalIF":5.6,"publicationDate":"2026-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145881524","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Recent advances in hydrogel therapy for traumatic brain injury 水凝胶治疗外伤性脑损伤的最新进展。

IF 5.6 2区医学

Colloids and Surfaces B: Biointerfaces Pub Date : 2026-04-01 Epub Date: 2026-01-06 DOI: 10.1016/j.colsurfb.2026.115424

Zheng Zou , Mengjia Chen , Jun Liu , Huan Ma , Guobiao Liang , Jingyuan Li

{"title":"Recent advances in hydrogel therapy for traumatic brain injury","authors":"Zheng Zou , Mengjia Chen , Jun Liu , Huan Ma , Guobiao Liang , Jingyuan Li","doi":"10.1016/j.colsurfb.2026.115424","DOIUrl":"10.1016/j.colsurfb.2026.115424","url":null,"abstract":"<div><div>Traumatic brain injury (TBI) remains one of the most devastating neurological disorders, characterized by complex primary mechanical damage and secondary cascades involving inflammation, oxidative stress, and glial scar formation. Conventional therapies offer limited efficacy due to the blood–brain barrier, the inability to reconstruct tissue defects, and poor spatiotemporal drug control. Hydrogels have emerged as a versatile platform for brain repair owing to their high-water content, biocompatibility, tunable mechanics, and injectability. By tailoring their composition and crosslinking mechanisms, hydrogels can mimic the native brain extracellular matrix, fill irregular cavities, and provide mechanical support that matches neural tissue softness. More importantly, functional hydrogels serve as local delivery vehicles for neuroprotective drugs and growth factors, scaffolds for stem cell transplantation, and active regulators of the post-injury microenvironment. Recent advances include bioactive, conductive, and stimuli-responsive hydrogels capable of modulating immune polarization, enhancing angiogenesis, and promoting neurogenesis. Despite encouraging preclinical results, challenges remain in achieving long-term biocompatibility, precise degradation control, and scalable clinical translation. This review summarizes the current progress, underlying mechanisms, and emerging design strategies of hydrogel-based therapies for TBI, highlighting their potential as next-generation biomaterials for neuroregeneration and functional recovery.</div></div>","PeriodicalId":279,"journal":{"name":"Colloids and Surfaces B: Biointerfaces","volume":"260 ","pages":"Article 115424"},"PeriodicalIF":5.6,"publicationDate":"2026-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145931687","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Integrated analysis of single-cell and bulk RNA-seq data identifies NR4A1-associated macrophages in immunoglobulin A nephropathy 单细胞和大量RNA-seq数据的综合分析确定了免疫球蛋白A肾病中nr4a1相关的巨噬细胞。

IF 5.6 2区医学

Colloids and Surfaces B: Biointerfaces Pub Date : 2026-04-01 Epub Date: 2025-12-23 DOI: 10.1016/j.colsurfb.2025.115395

Xueqin Zhang , Peng Chao , Lei Zhang, Yang Zhao, Hui Li, Yipeng Zhou, Xue Song, Shufen Yang, Gulimire Muhetaer, Hong Jiang, Chen Lu

{"title":"Integrated analysis of single-cell and bulk RNA-seq data identifies NR4A1-associated macrophages in immunoglobulin A nephropathy","authors":"Xueqin Zhang , Peng Chao , Lei Zhang, Yang Zhao, Hui Li, Yipeng Zhou, Xue Song, Shufen Yang, Gulimire Muhetaer, Hong Jiang, Chen Lu","doi":"10.1016/j.colsurfb.2025.115395","DOIUrl":"10.1016/j.colsurfb.2025.115395","url":null,"abstract":"<div><div>Immunoglobulin A nephropathy (IgAN), the most prevalent primary glomerulonephritis worldwide, is characterized by chronic renal inflammation and progressive decline toward end-stage renal disease. In this study, we integrated single-cell and bulk RNA sequencing approaches to systematically elucidate the role of macrophages in IgAN pathogenesis. Single-cell transcriptomic analysis identified 171 macrophage-associated differentially expressed genes and revealed two distinct molecular subtypes with divergent immune and metabolic signatures. Using machine learning algorithms, we constructed a diagnostic model based on eight macrophage-related markers, including NR4A1, which demonstrated robust predictive accuracy (Area Under the Curve > 0.9) across multiple independent validation cohorts. Functional experiments further established NR4A1 as a protective factor in IgAN. Overexpression of NR4A1 in an IgAN mouse model markedly reduced renal fibrosis, macrophage infiltration, and glomerular injury, whereas NR4A1 knockout exacerbated these pathological features. Mechanistically, the transcription factor ZBTB7A, which is upregulated in IgAN, directly suppresses NR4A1 expression through protein interaction. Rescue experiments demonstrated that NR4A1 overexpression mitigates ZBTB7A-induced renal damage. Collectively, these findings identify the ZBTB7A–NR4A1 regulatory axis as a critical mechanism driving IgAN progression and highlight NR4A1 as a potential diagnostic biomarker and therapeutic target for disease prevention.</div></div>","PeriodicalId":279,"journal":{"name":"Colloids and Surfaces B: Biointerfaces","volume":"260 ","pages":"Article 115395"},"PeriodicalIF":5.6,"publicationDate":"2026-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145831813","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Screening and application of a coagulation peptide-KFVLK with rapid hemostatic property 具有快速止血作用的凝血肽kfvlk的筛选与应用。

IF 5.6 2区医学

Colloids and Surfaces B: Biointerfaces Pub Date : 2026-04-01 Epub Date: 2025-12-19 DOI: 10.1016/j.colsurfb.2025.115387

Xin Liu , Zekai Ren , Xin Ding , Han Wu , Yumei Wang , Yang Cao , Hailin Cong , Bing Yu

{"title":"Screening and application of a coagulation peptide-KFVLK with rapid hemostatic property","authors":"Xin Liu , Zekai Ren , Xin Ding , Han Wu , Yumei Wang , Yang Cao , Hailin Cong , Bing Yu","doi":"10.1016/j.colsurfb.2025.115387","DOIUrl":"10.1016/j.colsurfb.2025.115387","url":null,"abstract":"<div><div>Wound hemostasis and healing have been important issues faced in wound therapy. Bioactive peptides are widely used in wound therapy due to their good biocompatibility and tunable functionality. Here we prepared Erythrocyte Membrane Chromatography Packing Material (EMCPM) by combining SiO<sub>2</sub> stationary-phase microspheres and cell membranes of erythrocytes through diazotized resin, and screened the coagulation peptide KFVLK from mobile phase peptide libraries, which lyophilized in the form of white solid particles with good biosafety, and could rapidly adsorb erythrocytes and platelets to stop wound bleeding. In the liver hemorrhage model, it could stop wound bleeding within 20 s, which reduced blood loss by 50 % compared with the control group. Meanwhile, it has good performance in promoting wound healing, with a healing rate of 88 % on fibroblasts within 24 h. The wound healing model also verified its performance. In conclusion, this screening method is of great significance for the preparation of coagulation peptides and hemostatic treatment of wounds.</div></div>","PeriodicalId":279,"journal":{"name":"Colloids and Surfaces B: Biointerfaces","volume":"260 ","pages":"Article 115387"},"PeriodicalIF":5.6,"publicationDate":"2026-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145831844","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0