Colloids and Surfaces B: Biointerfaces最新文献

{"title":"Recent progress in alginate-based nanocomposites for bone tissue engineering applications","authors":"Sundaravadhanan Lekhavadhani,&nbsp;Sushma Babu,&nbsp;Abinaya Shanmugavadivu,&nbsp;Nagarajan Selvamurugan","doi":"10.1016/j.colsurfb.2025.114570","DOIUrl":"10.1016/j.colsurfb.2025.114570","url":null,"abstract":"<div><div>Approximately 5–10 % of fractures are associated with non-union, posing a significant challenge in orthopedic applications. Addressing this issue, innovative approaches beyond traditional grafting techniques like bone tissue engineering (BTE) are required. Biomaterials, combined with cells and bioactive molecules in BTE, are critical in managing non-union. Alginate, a natural polysaccharide, has gained widespread recognition in bone regeneration due to its bioavailability, its ability to form gels through crosslinking with divalent cations, and its cost-effectiveness. However, its inherent mechanical weaknesses necessitate a combinatorial approach with other biomaterials. In recent years, nanoscale biomaterials have gained prominence for bone regeneration due to their structural and compositional resemblance to natural bone, offering a supportive environment that regulates cell proliferation and differentiation for new bone formation. In this review, we briefly outline the synthesis of alginate-based nanocomposites using different fabrication techniques, such as hydrogels, 3D-printed scaffolds, fibers, and surface coatings with polymer, ceramic, carbon, metal, or lipid-based nanoparticles. These alginate-based nanocomposites elicit angiogenic, antibacterial, and immunomodulatory properties, thereby enhancing the osteogenic potential as an insightful measure for treating non-union. Despite the existence of similar literature, this work delivers a recent and focused examination of the latest advancements and insights on the potential of alginate-based nanocomposites for BTE applications. This review also underscores the obstacles that alginate-based nanocomposites must overcome to successfully transition into clinical applications.</div></div>","PeriodicalId":279,"journal":{"name":"Colloids and Surfaces B: Biointerfaces","volume":"250 ","pages":"Article 114570"},"PeriodicalIF":5.4,"publicationDate":"2025-02-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143437439","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effect of polysaccharides on the inhibition and binding ability of hesperetin-copper(II) complex on α-glucosidase","authors":"Xi Peng ,&nbsp;Yushi Wei ,&nbsp;Yijing Liao ,&nbsp;Xing Hu ,&nbsp;Deming Gong ,&nbsp;Guowen Zhang","doi":"10.1016/j.colsurfb.2025.114564","DOIUrl":"10.1016/j.colsurfb.2025.114564","url":null,"abstract":"<div><div>The study aimed to investigate the inhibitory effect of hesperetin-copper (II) [Hsp-Cu(II)] on α-glucosidase in the presence of polysaccharides (xylan, β-glucan, low-, medium- and high-viscosity chitosan). The results showed that all the polysaccharides significantly reduced the inhibitory activity of α-glucosidase by Hsp-Cu(II), and the reduction effect of high-viscosity chitosan was the most significant. The polysaccharides significantly decreased the binding constant of Hsp-Cu(II)<img>α-glucosidase, changed the binding sites of Hsp-Cu(II) to α-glucosidase and reduced the hydrogen bonds of Hsp-Cu(II) bound with α-glucosidase. Circular dichroism showed that the reduction of α-helix content in α-glucosidase caused by Hsp-Cu(II) was raised from 27.2 % to 29.5 %, 31.3 % and 32.7 % in the presence of xylan, β-glucan and high-viscosity chitosan, respectively, suggesting that the polysaccharides could restore the secondary structure of α-glucosidase. Fourier transforms infrared spectra showed that xylan and β-glucan formed hydrogen bonds with Hsp-Cu(II). The mechanism of the decreasing effect might be that the polysaccharides with the low viscosity compete with α-glucosidase to bind Hsp-Cu(II) through hydrogen bonds, restoring the catalytic center and active amino acid residues of Hsp-Cu(II) bound with α-glucosidase and the adsorption of high-viscosity chitosan decreases the binding affinity of Hsp-Cu(II) on α-glucosidase. The study may offer a reference for the development of Hsp-Cu(II)-based nutritional and healthy food for patients with hyperglycemia.</div></div>","PeriodicalId":279,"journal":{"name":"Colloids and Surfaces B: Biointerfaces","volume":"250 ","pages":"Article 114564"},"PeriodicalIF":5.4,"publicationDate":"2025-02-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143429616","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Revisiting the characteristics of nanomaterials, composites, hybrid and functionalized materials in medical microbiology","authors":"Jennifer Mariana Vargas López ,&nbsp;José Alfonso Cruz Ramos ,&nbsp;Gregorio Guadalupe Carbajal Arizaga","doi":"10.1016/j.colsurfb.2025.114556","DOIUrl":"10.1016/j.colsurfb.2025.114556","url":null,"abstract":"<div><div>Unlike traditional materials designed to form large structures, many modern materials are presented in the form of powders resulting from a molecular level control of their composition and structure, making possible the miniaturization and fine-tuning of their properties to act in cellular dimensions with customized tasks. Several new materials for biomedical and microbiology applications appear every year. Although many of them are called nanomaterials, there may be a more precise description or classification. In this work, we review and detail the structural classification of nanometric, functionalized, hybrid and composite materials, mainly based on descriptions given by the International Union of Pure and Applied Chemistry (IUPAC). Besides we included smart and multifunctional materials, cassification based on performance. The second section shows how these materials are used in the area of medical microbiology, grouping these applications into barriers for microorganisms on surfaces, disinfectants in clinical practice, targeting of pathogens, detectors of microorganisms or their metabolites, and also as substrates to stabilize, transport, or nourish beneficial microorganisms. Finally, we will discuss some evidence that indicates the environmental risk and bacterial resistance alerts that should be taken into account with the use of these advanced powder materials.</div></div>","PeriodicalId":279,"journal":{"name":"Colloids and Surfaces B: Biointerfaces","volume":"250 ","pages":"Article 114556"},"PeriodicalIF":5.4,"publicationDate":"2025-02-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143419145","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Proteomic evaluation of borosilicate hybrid sol-gel coatings with osteogenic, immunomodulatory and antibacterial properties","authors":"Francisco Romero-Gavilán ,&nbsp;Andreia Cerqueira ,&nbsp;Iñaki García-Arnáez ,&nbsp;Loredana Scalschi ,&nbsp;Begonya Vicedo ,&nbsp;Mikel Azkargorta ,&nbsp;Félix Elortza ,&nbsp;Raúl Izquierdo ,&nbsp;Mariló Gurruchaga ,&nbsp;Isabel Goñi ,&nbsp;Julio Suay","doi":"10.1016/j.colsurfb.2025.114561","DOIUrl":"10.1016/j.colsurfb.2025.114561","url":null,"abstract":"<div><div>Silica hybrid sol-gel coatings represent an interesting approach to bioactivate dental implants. Boron is known for its osteogenic, angiogenic and antibacterial functions in biomedical applications. This study describes the synthesis of a novel borosilicate hybrid sol-gel coating using a mixture of methyltrimethoxysilane, tetraethyl orthosilicate and trimethyl borate (TMB). Coatings with different amounts of boron were obtained, and their physiochemical properties were examined; <em>in vitro</em> tests with human osteoblasts and macrophages (THP-1) were carried out. The effects of these materials on bacteria viability were evaluated using <em>Escherichia coli</em> and <em>Staphylococcus aureus</em>. The human serum proteins adsorbed onto the coatings were analysed employing proteomic techniques. To synthesise the new materials, the appropriate sol-gel reactions were developed; boron was integrated into the silica network, and well-adhering coatings were obtained. These borosilicate coatings were non-cytotoxic, displayed osteogenic potential, and upregulated adsorption of proteins related to bone regeneration (IGF2, ALS and APOE). Boron upregulated the expression of TNF-α, INFg and TGF-β and increased the TNF-α and TGF-β cytokine production in THP-1. Moreover, the addition of boron caused downregulation of NOX2 expression. Proteomic analysis revealed that boron-doping reduced the adsorption of immunoglobulins and complement system proteins. It also caused an increase in the levels of apolipoproteins, antioxidant proteins and serum amyloid A proteins, which was in agreement with <em>in vitro</em> results. The coatings with 10 and 20 % TMB displayed antibacterial effect against <em>S. aureus</em>. The results of this study will enhance our comprehension of interactions between boron-containing biomaterials and biological systems.</div></div>","PeriodicalId":279,"journal":{"name":"Colloids and Surfaces B: Biointerfaces","volume":"250 ","pages":"Article 114561"},"PeriodicalIF":5.4,"publicationDate":"2025-02-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143419225","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Cobalt carbonate nanorods enhance chemotherapy via neutralization of acidic tumor microenvironment and generation of carbonate radical anions for necrosis","authors":"Min Wu ,&nbsp;Bing Liang ,&nbsp;Lu Zhang ,&nbsp;Benmeng Wu ,&nbsp;Jingjing Liu","doi":"10.1016/j.colsurfb.2025.114563","DOIUrl":"10.1016/j.colsurfb.2025.114563","url":null,"abstract":"<div><div>One of the hallmarks of cancer is the acidic extracellular space surrounding the tumor, which is linked to metabolic reprogramming and the use of glycolysis. Additionally, the acidic tumor microenvironment (TME) establishes a physiological barrier called \"ion trapping\" and significantly lowers the ability of cells to absorb weak-base chemotherapy agents. Although CO₃^2- containing agents and nanoformulations could effectively neutralize the tumor acidity, the CO₃^2- based therapeutic effect was insufficiently investigated. Herein, we fabricated cobalt carbonate (CoCO₃) nanorods as drug carriers with acidity-responsive dissociation and acidity neutralization properties for the loading of hydrophobic and weak-basic drugs, evodiamine (EVO). After effective surface modification, CoCO₃-PEG-EVO could effectively accumulate in the tumor and inhibit the growth of the tumor. On the one hand, acidity neutralization of CoCO₃-PEG-EVO could lead to the ion trapping overcome and cellular uptake of EVO enhancement for effective cancer cell apoptosis. On the other hand, the high level of H₂O₂ in the tumor and HCO₃^- from dissociated CoCO₃-PEG-EVO could cause the generation of CO₃·^- through a Fenton-like reaction while not hydroxyl radical (·OH) for cancer cell necrosis. Our results thus point to a potent yet easily prepared CoCO₃ nanosystem (CoCO₃-PEG-EVO) to induce cancer cell death, and because of its well-defined composition and excellent biocompatibility, it may be used in clinical settings in the future.</div></div>","PeriodicalId":279,"journal":{"name":"Colloids and Surfaces B: Biointerfaces","volume":"250 ","pages":"Article 114563"},"PeriodicalIF":5.4,"publicationDate":"2025-02-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143444473","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Carbon nanoparticles-Fe(II) complex combined with sorafenib for ferroptosis-induced antitumor effects in triple-negative breast cancer","authors":"Ping Xie ,&nbsp;Ting Qu ,&nbsp;Kexin Tang ,&nbsp;Yuanfang Huang ,&nbsp;Guangfu Zeng ,&nbsp;Huahui Yuan ,&nbsp;Qian Xin ,&nbsp;Yufeng Zhao ,&nbsp;Jinmei Yang ,&nbsp;Cheng Zeng ,&nbsp;Xian Wu ,&nbsp;Sheng-Tao Yang ,&nbsp;Xiaohai Tang","doi":"10.1016/j.colsurfb.2025.114562","DOIUrl":"10.1016/j.colsurfb.2025.114562","url":null,"abstract":"<div><div>Triple negative breast cancer (TNBC) represents an aggressive subtype of breast cancer that lacks the expression of estrogen receptor, progesterone receptor, and human epidermal growth factor receptor 2, whose systemic treatment options are currently limited to chemotherapy. Carbon nanoparticles-Fe(II) complex (CNSI-Fe) is a promising antitumor drug that induces ferroptosis to kill tumor cells efficiently. In this study, we combined CNSI-Fe and a ferroptosis inducer sorafenib (SRF) to achieve the efficient chemotherapy of TNBC. CNSI-Fe could adsorb SRF by hydrophobic interaction and π-π stacking with a maximum adsorption capacity of 31 mg/g. During the in vitro assays, CNSI-Fe+SRF combination inhibited the cell viability of 4T1 cells much more efficiently than CNSI-Fe or SRF alone. The high Fe uptake, hydroxyl radical generation and oxidative damages verified the ferroptosis of 4T1 cells upon the CNSI-Fe+SRF treatment. During the in vivo evaluations, SRF enhanced the therapeutic effect of CNSI-Fe as indicated by the higher tumor growth inhibition rate of 67.8 % and the higher survival rate. CNSI captured SRF in tumor to give a 6 mg/kg uptake, which lowered the glutathione peroxidase 4 (GPX4) level and enhanced the hydroxyl radical production of 4T1 tumor. In addition, CNSI-Fe treatment up-regulated the genes associated with antioxidative responses, but the up-regulation was offset by SRF. CNSI-Fe+SRF group showed similar toxicity to mice as SRF alone in the biosafety evaluations. Our results collectively indicated that the combination of CNSI-Fe and SRF could efficiently treat TNBC through ferroptosis.</div></div>","PeriodicalId":279,"journal":{"name":"Colloids and Surfaces B: Biointerfaces","volume":"250 ","pages":"Article 114562"},"PeriodicalIF":5.4,"publicationDate":"2025-02-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143419146","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Antibacterial and bactericidal properties of resin nanostructures coated with SiO2 thin films","authors":"Yuito Matsushita ,&nbsp;Gakuto Inoue ,&nbsp;Zihao Zhao ,&nbsp;Natsuki Ogawa ,&nbsp;Hitoshi Ishiguro ,&nbsp;Kayano Sunada ,&nbsp;Kenta Ishibashi ,&nbsp;Hiroaki Kojima ,&nbsp;Tomohiro Shimizu ,&nbsp;Shoso Shingubara ,&nbsp;Takeshi Ito","doi":"10.1016/j.colsurfb.2025.114560","DOIUrl":"10.1016/j.colsurfb.2025.114560","url":null,"abstract":"<div><div>Nanotextures exhibit physical antibacterial and bactericidal properties; hence, they have great potential to prevent infection related to bacteria through contact. The surface characteristics of a nanostructure determine its antibacterial and bactericidal activities. In this study, an atomic layer deposition (ALD) was used to prepare nano-level, hard, thin SiO₂ layers on resin nanostructures and their effects were demonstrated through antibacterial and bactericidal tests. The SiO₂-layer-coated resin nanostructure exhibited a water contact angle of 7.2°, dramatically lower than that of the uncoated specimen (130.2°), as well as a three-fold higher local elastic modulus. Further, 10-nm-thin SiO₂-layer-coated nanopillars showed antibacterial and bactericidal effects against <em>E. coli</em>. These results demonstrate that SiO₂ thin layer coating has great potential for improving the antibacterial and bactericidal properties of polymeric nanopillar arrays.</div></div>","PeriodicalId":279,"journal":{"name":"Colloids and Surfaces B: Biointerfaces","volume":"250 ","pages":"Article 114560"},"PeriodicalIF":5.4,"publicationDate":"2025-02-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143419147","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A computer-aided, carrier-free drug delivery system with enhanced cytotoxicity and biocompatibility: A universal model for multifunctional lung cancer therapy","authors":"Zhonglei Wang ,&nbsp;Wenjing Xu ,&nbsp;Shizeng Lei ,&nbsp;Yuxin Lai ,&nbsp;Yingying Zhang ,&nbsp;Ying Wang ,&nbsp;Ziling Xiang ,&nbsp;Xin Fu ,&nbsp;Liyan Yang","doi":"10.1016/j.colsurfb.2025.114557","DOIUrl":"10.1016/j.colsurfb.2025.114557","url":null,"abstract":"<div><div>Erlotinib (ERL) is a first-line targeted therapy for patients with epidermal growth factor receptor (EGFR)-mutant advanced non-small cell lung cancer (NSCLC). However, its effectiveness is hindered by acquired resistance and poor bioavailability. Carrier-free nanodrugs are a research hotspot due to their efficient targeting, high drug loading capacity, and the absence of any excipients. Herein, we report an advanced self-delivery system for multimodal NSCLC therapy using a computer-aided strategy. First, we developed a novel heterodimer, ERL-SS-QM (ERL conjugated with QM-OH—a hydrophobic aggregation-induced emission fluorophore—via a disulfide bond [SS]), which serves as both cargo and carrier material. Self-assembly is driven by multiple noncovalent interactions, including π-π stacking and sulfur bonds. Subsequently, an ERL-SS-QM-based \"triadic\" drug delivery nanoplatform comprising 21 variants was developed. A case study on ursolic acid (UA)-loaded ERL-SS-QM nanoparticles (named UA@ERL-SS-QM NPs) revealed narrow size distribution, small particle size, and well stabilized (zeta potential = −28.9 mV). The UA@ERL-SS-QM NPs demonstrated concentration-dependent toxicity against targeted A549 cells (IC₅₀ = 4.36 μM), outperforming free monomeric drugs ERL (IC₅₀ = 12.94 μM) and UA (IC₅₀ = 12.21 μM), indicating good efficiency. Conversely, these NPs exhibited minimal cytotoxicity in non-targeted BEAS-2B cells, suggesting favorable biocompatibility. Upon endocytosis and interaction with overexpressed GSH in A549 cells, the disulfide-bond linker is cleaved to release three components: ERL, UA (which downregulates β-catenin/TCF4/CT45A2 signaling pathways, inducing apoptosis in ERL-resistant L858R/T790M mutant cells—a key factor in acquired resistance to ERL treatment), and QM-OH. Hence, this work provides a universal model for multifunctional NSCLC therapy that effectively addresses ERL resistance while enhancing cytotoxicity and biocompatibility.</div></div>","PeriodicalId":279,"journal":{"name":"Colloids and Surfaces B: Biointerfaces","volume":"250 ","pages":"Article 114557"},"PeriodicalIF":5.4,"publicationDate":"2025-02-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143377214","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Human amniotic mesenchymal stem cells improve patency and regeneration of electrospun biodegradable vascular grafts via anti-thrombogenicity and M2 macrophage polarization","authors":"Nuoxin Wang ,&nbsp;Haoyuan Wang ,&nbsp;Dong Weng ,&nbsp;Bin Wang ,&nbsp;Juan Wang ,&nbsp;Jing He ,&nbsp;Xiumei Mo ,&nbsp;Feng Wang ,&nbsp;Zhixu He","doi":"10.1016/j.colsurfb.2025.114559","DOIUrl":"10.1016/j.colsurfb.2025.114559","url":null,"abstract":"<div><div>Small-diameter vascular grafts (SDVGs) are prone to thrombosis and have low long-term patency rates for various reasons, which cannot meet the clinical requirements. In this work, Human amniotic mesenchymal stem cell (hAMSC) seeding electrospun polylactic acid-co-polycaprolactone (PLCL) SDVGs are fabricated and their application potential is systematically evaluated. The SDVG has excellent mechanical properties. PLCL eletrospinning membrane has no cytotoxicity. The SDVG has a porous fibrous tube wall, uniform distribution of hAMSCs, and good cell compatibility, blood compatibility, histocompatibility and mechanical properties. hAMSCs loading can improve the acute antithrombotic ability, patency and in vivo regeneration effect of PLCL electrospun SDVGs. The mechanism is related to hAMSCs increasing the content of endothelial cells, contractile smooth muscle cells, and M2 macrophages, as well as activating extracellular matrix production.</div></div>","PeriodicalId":279,"journal":{"name":"Colloids and Surfaces B: Biointerfaces","volume":"250 ","pages":"Article 114559"},"PeriodicalIF":5.4,"publicationDate":"2025-02-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143402876","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Free-standing, flexible and conformable bilayered polymeric nanomembranes modified with gold nanomaterials as electronic skin sensors","authors":"Samuele Colombi ,&nbsp;Carlos Alemán ,&nbsp;Jose García-Torres","doi":"10.1016/j.colsurfb.2025.114558","DOIUrl":"10.1016/j.colsurfb.2025.114558","url":null,"abstract":"<div><div>Skin is a barrier that protects us against physical, chemical and biological agents. However, any damage to the skin can disrupt this barrier and therefore compromise its function leading to sometimes catastrophic consequences like sepsis. Thus, methods to detect early signs of infection are necessary. In this work, we have developed a straightforward method for producing 2D nanomembranes with regularly spaced 1D metallic nanostructures integrating sensing capabilities to pH and NADH (nicotinamide adenine dinucleotide), which are critical analytes revealing infection. To achieve this, we have successfully fabricated a bilayered nanomembrane combining a pH-responsive polyaniline (PANI) layer and a nanoperforated poly(lactic acid) (PLA) layer containing gold nanowires (Au NWs) as NADH sensing element. SEM, FTIR, Raman and AFM techniques revealed the formation of the bilayered PANI/PLA nanomembrane and the successful incorporation of the Au NWs inside the nanoperforations. The resulting bilayered nanomembrane showed significant flexibility and conformability onto different substrates due to the softness of the polymers and the ultrathin thickness with stiffness values similar to human skin. These nanomembranes also exhibited remarkable electrochemical sensing performance towards pH and NADH detection. Thus, the nanomembrane displayed linearity with good sensitivity (47 mV pH⁻¹) in the critical pH range 4–10 and fast response time (10 s). On the other hand, PANI/PLA-Au nanomembranes also allowed the quantitative sensing of NADH with a limit of detection of 0.39 mM and a sensitivity of 1 µA cm⁻² mM⁻¹ in the concentration range 0–5 mM.</div></div>","PeriodicalId":279,"journal":{"name":"Colloids and Surfaces B: Biointerfaces","volume":"250 ","pages":"Article 114558"},"PeriodicalIF":5.4,"publicationDate":"2025-02-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143395539","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}