Colloids and Surfaces B: Biointerfaces最新文献_第10页

Enhanced transcytosis and therapeutic efficacy of paclitaxel nanoparticles: Pyridylboronic acid modification and sialic acid targeting.

IF 5.4 2区医学

Colloids and Surfaces B: Biointerfaces Pub Date : 2024-11-30 DOI: 10.1016/j.colsurfb.2024.114417

Manzhen Li, Miao Chen, Pengxin Li, Ziqi Zhang, Han Yu, Xiangtao Wang

{"title":"Enhanced transcytosis and therapeutic efficacy of paclitaxel nanoparticles: Pyridylboronic acid modification and sialic acid targeting.","authors":"Manzhen Li, Miao Chen, Pengxin Li, Ziqi Zhang, Han Yu, Xiangtao Wang","doi":"10.1016/j.colsurfb.2024.114417","DOIUrl":"https://doi.org/10.1016/j.colsurfb.2024.114417","url":null,"abstract":"<p><p>Efficient drug delivery and deeper penetration into tumors have become a primary focus of anti-tumor nanomedicine. In this study, pyridylboronic acid (BPA), as a targeting ligand for sialic acid, which is highly expressed on the surface of tumor cells, was conjugated with DSPE-PEG2k-NH<sub>2</sub> to synthesize DSPE-PEG2k-BPA and used to encapsulate PTX. The resultant PTX@DSPE-PEG2k-BPA nanoparticles (DPB NPs) showed a mean particle size of 189.0 ± 3.5 nm, with a high drug loading content of 48.75 % and a rod-like morphology. In contrast to PTX@DSPE-mPEG2k nanoparticles (DP NPs), DPB NPs displayed enhanced cellular uptake and targetability to 4T1 tumor cells. Interestingly, BPA modification could also enhance transcytosis through the endoplasmic reticulum-Golgi pathway, thus improving penetration and accumulation of nanoparticles in tumors. An in vivo study on 4T1 tumor-bearing mice demonstrated that DPB NPs achieved a faster and more accumulation in tumors than DP NPs after intravenous administration, led to significantly improved therapeutic efficacy with a higher tumor inhibition rate (74.27 % vs 50.58 %, p < 0.01). In conclusion, the modification of BPA presents a strategy for the development of drug delivery systems that exhibit dual functionalities: active targeting and transcytosis.</p>","PeriodicalId":279,"journal":{"name":"Colloids and Surfaces B: Biointerfaces","volume":"247 ","pages":"114417"},"PeriodicalIF":5.4,"publicationDate":"2024-11-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142778695","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Pectin/caffeic acid nanoparticles in a poloxamer thermosensitive gel for the treatment of ulcerative colitis by inhibiting cGAS-STING pathway.

IF 5.4 2区医学

Colloids and Surfaces B: Biointerfaces Pub Date : 2024-11-30 DOI: 10.1016/j.colsurfb.2024.114419

Shilin Zhou, Zhenxin Yu, Wenqing Yao, Mengdi Wang, Yongqiang Yang, Jien Qin, Xiaochen Wu, Chuanlong Guo

{"title":"Pectin/caffeic acid nanoparticles in a poloxamer thermosensitive gel for the treatment of ulcerative colitis by inhibiting cGAS-STING pathway.","authors":"Shilin Zhou, Zhenxin Yu, Wenqing Yao, Mengdi Wang, Yongqiang Yang, Jien Qin, Xiaochen Wu, Chuanlong Guo","doi":"10.1016/j.colsurfb.2024.114419","DOIUrl":"https://doi.org/10.1016/j.colsurfb.2024.114419","url":null,"abstract":"<p><p>Ulcerative colitis is a recurring condition that causes inflammation and sores in the digestive system. Current clinical treatments for ulcerative colitis have limitations due to side effects and poor patient compliance. This study investigates the therapeutic potential of a novel drug delivery system, CA-Gel, which comprises caffeic acid (CA) stabilized by pectin nanoparticles within a poloxamer thermosensitive gel for rectal administration. The system aims to provide controlled and sustained release of CA directly to the colon. In vitro studies demonstrated that CA-Gel exhibited excellent biocompatibility, cytoprotective effects, and reduced oxidative stress and cellular apoptosis. In vivo studies using a dextran sulfate sodium (DSS)-induced colitis mouse model showed that CA-Gel significantly alleviated colitis symptoms, as evidenced by improvements in body weight, disease activity index (DAI), colon length, and histopathological assessments. Additionally, CA-Gel modulated the Cyclic GMP AMP synthase (cGAS)-stimulator of interferon genes (STING) pathway, reduced mitochondrial DNA (mtDNA) release, and inhibited inflammatory cytokines, thereby demonstrating its therapeutic potential in ulcerative colitis. The study concludes that CA-Gel is a promising rectal treatment for ulcerative colitis, offering a safe and effective alternative to existing pharmacological therapies.</p>","PeriodicalId":279,"journal":{"name":"Colloids and Surfaces B: Biointerfaces","volume":"247 ","pages":"114419"},"PeriodicalIF":5.4,"publicationDate":"2024-11-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142764949","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Bioinspired synthesis of virus-like particle-templated thin silica-layered nanocages with enhanced biocompatibility and cellular uptake as drug delivery carriers.

IF 5.4 2区医学

Colloids and Surfaces B: Biointerfaces Pub Date : 2024-11-29 DOI: 10.1016/j.colsurfb.2024.114418

Kyeong Rok Kim, Ae Sol Lee, Hye Ryoung Heo, So-Young Park, Chang Sup Kim

{"title":"Bioinspired synthesis of virus-like particle-templated thin silica-layered nanocages with enhanced biocompatibility and cellular uptake as drug delivery carriers.","authors":"Kyeong Rok Kim, Ae Sol Lee, Hye Ryoung Heo, So-Young Park, Chang Sup Kim","doi":"10.1016/j.colsurfb.2024.114418","DOIUrl":"https://doi.org/10.1016/j.colsurfb.2024.114418","url":null,"abstract":"<p><p>The bioinspired synthesis of virus-like silica nanoparticles in biomedical applications makes it possible to utilize the cellular delivery capabilities of viruses while minimizing the cytotoxicity of inorganic silica. In this study, we developed a diatom-inspired method for synthesizing silica-layered nanocages utilizing R5 peptide-functionalized virus-like particles (VLPs). R5 peptides were genetically inserted into the F-G loop of human papillomavirus 16 L1 proteins (HPV16 L1-R5). HPV16 L1-R5 was self-assembled into VLPs under an acidic pH similar to native ones and exhibited ∼65 % drug encapsulation efficiency. The HPV16 L1-R5 VLP@silica nanocages (SiNPs) were synthesized through diatom-inspired silicification of HPV16 L1-R5 VLPs via intermolecular interaction of the R5 peptide and polyol. HPV16L1-R5 VLP@SiNPs displayed uniform, monodisperse particles with approximately 10 nm silica layer compared to HPV16 L1-R5 VLPs. HPV16 L1-R5 VLP@SiNPs showed high biocompatibility at high concentrations, unlike commercial mesoporous SiNPs. Furthermore, the virus-like HPV16 L1-R5 VLP@SiNPs resulted in approximately 2.5-fold increased cellular uptake efficiency compared to commercial mesoporous SiNPs. These results suggest that the thin silica layer on HPV16 L1-R5 VLPs retains cellular delivery capacity while reducing cytotoxicity. Our strategy presents an innovative method for synthesizing virus-like nanoparticles in biomedical applications, enhancing cellular delivery capacity and biocompatibility.</p>","PeriodicalId":279,"journal":{"name":"Colloids and Surfaces B: Biointerfaces","volume":"247 ","pages":"114418"},"PeriodicalIF":5.4,"publicationDate":"2024-11-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142790676","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Recent advances in biomimetic nanodelivery systems for the treatment of myocardial ischemia reperfusion injury.

IF 5.4 2区医学

Colloids and Surfaces B: Biointerfaces Pub Date : 2024-11-28 DOI: 10.1016/j.colsurfb.2024.114414

Xiaojun Bi, Ze Wang, Jingteng He

引用次数: 0

From quorum sensing inhibition to antimicrobial defense: The dual role of eugenol-gold nanoparticles against carbapenem-resistant Pseudomonas aeruginosa

IF 5.4 2区医学

Colloids and Surfaces B: Biointerfaces Pub Date : 2024-11-28 DOI: 10.1016/j.colsurfb.2024.114415

Huale Chen , Panjie Hu , Yaran Wang , Haifeng Liu , Junyuan Zheng , Zeyu Huang , Xiaotuan Zhang , Yong Liu , Tieli Zhou

{"title":"From quorum sensing inhibition to antimicrobial defense: The dual role of eugenol-gold nanoparticles against carbapenem-resistant Pseudomonas aeruginosa","authors":"Huale Chen , Panjie Hu , Yaran Wang , Haifeng Liu , Junyuan Zheng , Zeyu Huang , Xiaotuan Zhang , Yong Liu , Tieli Zhou","doi":"10.1016/j.colsurfb.2024.114415","DOIUrl":"10.1016/j.colsurfb.2024.114415","url":null,"abstract":"<div><div>To address the pressing challenge of antibiotic resistance, particularly the robust defense mechanisms of <em>Pseudomonas aeruginosa</em> (<em>P. aeruginosa</em>) against conventional antibiotics, this study employs nanotechnology to enhance antimicrobial efficacy while ensuring good biocompatibility with the host. In this study, gold nanoparticles were chemically decorated with eugenol, a phenol-rich natural compound, using a one-pot synthesis method. The successful synthesis and functionalization of eugenol-decorated gold nanoparticles (Eugenol_Au NPs) were validated by comprehensive physicochemical analyses, demonstrating their stability and biocompatibility. These nanoparticles exhibited potent antimicrobial activity against both planktonic and biofilm-embedded carbapenem-resistant <em>P. aeruginosa</em> strains. Eugenol_Au NPs disrupted the bacterial quorum sensing system and stimulated intracellular reactive oxygen species production, which enhance their antibacterial effects. This dual mechanism of action has promising clinical implications for the treatment of infections associated with antibiotic-resistant <em>P. aeruginosa</em>. <em>In vivo</em> assessments in a murine peritoneal infection model showed that Eugenol_Au NPs significantly reduced bacterial loads and mitigated inflammatory responses, thereby improving survival rates. The study highlights the potential of Eugenol_Au NPs as an alternative strategy for refractory infections caused by carbapenem-resistant <em>P. aeruginosa</em>, and underscores the feasibility and promise of further clinical research and development of new therapeutic approaches targeting this resistant pathogen.</div></div>","PeriodicalId":279,"journal":{"name":"Colloids and Surfaces B: Biointerfaces","volume":"247 ","pages":"Article 114415"},"PeriodicalIF":5.4,"publicationDate":"2024-11-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142757241","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Durable and biocompatible low adhesion wound dressing material based on interfacial behaviors for wound management

IF 5.4 2区医学

Colloids and Surfaces B: Biointerfaces Pub Date : 2024-11-27 DOI: 10.1016/j.colsurfb.2024.114413

Guohao Li , Zuo Pu , Shuang Guo , Zhuopeng Liu , Maosen Deng , Na Liu , Zhe Li

{"title":"Durable and biocompatible low adhesion wound dressing material based on interfacial behaviors for wound management","authors":"Guohao Li , Zuo Pu , Shuang Guo , Zhuopeng Liu , Maosen Deng , Na Liu , Zhe Li","doi":"10.1016/j.colsurfb.2024.114413","DOIUrl":"10.1016/j.colsurfb.2024.114413","url":null,"abstract":"<div><div>Wound-dressing adhesion is a problem that has not been effectively addressed in the field of wound care for bleeding or burn wounds. Design of low adhesion wound dressing materials by leveraging interfacial behaviors has been an effective solution to this problem. However, previously reported superhydrophobic low adhesion materials either had durability or biocompatibility issue. To bridge this gap, this study presents a durable and biocompatible superhydrophobic low adhesion wound dressing material, which is designed on a normal gauze substrate with biocompatible components using a hybrid coating strategy. Outstanding low adhesion properties have been verified <em>in vivo</em> with bleeding wound or burn wound, with a peeling force that is only 0.3 %-14.5 % of the conventional non-woven gauze. Prepared low adhesion materials can robustly retain their superhydrophobicity and blood-repelling properties against harsh tests. Moreover, their biocompatibility has been confirmed through a series of tests including cell biocompatibility, hemolysis and skin irritation tests. With these demonstrated merits, the durable and biocompatible low adhesion material developed in this study will provide an effective solution to the wound adhesion problem in the practice of wound management.</div></div>","PeriodicalId":279,"journal":{"name":"Colloids and Surfaces B: Biointerfaces","volume":"247 ","pages":"Article 114413"},"PeriodicalIF":5.4,"publicationDate":"2024-11-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142754396","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Polyphenol enhances the functionality of borate hydrogel in wound repair by regulating the wound microenvironment.

IF 5.4 2区医学

Colloids and Surfaces B: Biointerfaces Pub Date : 2024-11-27 DOI: 10.1016/j.colsurfb.2024.114390

Liang Quan, Yuan Xin, Hengtong Zhang, Xixi Wu, Xiaoyun Li, Chen Zhou, Qiang Ao

{"title":"Polyphenol enhances the functionality of borate hydrogel in wound repair by regulating the wound microenvironment.","authors":"Liang Quan, Yuan Xin, Hengtong Zhang, Xixi Wu, Xiaoyun Li, Chen Zhou, Qiang Ao","doi":"10.1016/j.colsurfb.2024.114390","DOIUrl":"https://doi.org/10.1016/j.colsurfb.2024.114390","url":null,"abstract":"<p><p>Wound infections represent a significant clinical challenge. In this study, a polyphenol (tannic acid, TA)-enhanced borate hydrogel modulating the tissue microenvironment to promote wound healing was designed as an antimicrobial hydrogel. The physical properties of the multiply cross-linked borate hydrogel were analyzed using a combination of techniques, including FT-IR, <sup>1</sup>H NMR, SEM, and rheological analysis. The combination of polyvinyl alcohol (PVA), phenylboronic acid-functionalized chitosan (N-PBACS), and TA resulted in the formation of multi-crosslinked networks (PVA@N-PBACS, TA@PVA, and TA@N-PBACS) that markedly enhanced the hydrogel's mechanical strength, deformability (compression and tensile), and adhesion properties. The multi-crosslinked hydrogels exhibited broad-spectrum antimicrobial activity and antioxidant effects in vitro, as well as excellent biocompatibility and the promotion of cell proliferation, migration and vascularisation behaviours. The in vivo results demonstrated that the hydrogel had enhanced properties. Furthermore, it exhibits good biocompatibility, reactive oxygen species (ROS) scavenging ability, antimicrobial properties, and the ability to modulate immune status. In an in vivo bacterial infection model, the multi-crosslinked hydrogel effectively modulated the wound microenvironment through antimicrobial effects, oxidative stress, ROS levels, and immunity modulation. This study offers a promising solution for improving wound care and provides insight into potential future therapeutic strategies.</p>","PeriodicalId":279,"journal":{"name":"Colloids and Surfaces B: Biointerfaces","volume":"247 ","pages":"114390"},"PeriodicalIF":5.4,"publicationDate":"2024-11-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142783553","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

ROS-responsive supramolecular antimicrobial peptides-based nanoprodrugs for cervical cancer therapy

IF 5.4 2区医学

Colloids and Surfaces B: Biointerfaces Pub Date : 2024-11-26 DOI: 10.1016/j.colsurfb.2024.114411

Yanzhu Pan, Zhongxiong Fan, Shaoqi Yu, Lijie Xia, Jinyao Li

{"title":"ROS-responsive supramolecular antimicrobial peptides-based nanoprodrugs for cervical cancer therapy","authors":"Yanzhu Pan, Zhongxiong Fan, Shaoqi Yu, Lijie Xia, Jinyao Li","doi":"10.1016/j.colsurfb.2024.114411","DOIUrl":"10.1016/j.colsurfb.2024.114411","url":null,"abstract":"<div><div>Although antimicrobial peptides (AMPs) as a promising natural drugs can efficiently inhibit cervical cancer, poor bioavailability, low tumor selectivity, and non-selective toxicity still hinder its further application in vivo. In order to effectively address these challenges, we have developed a reactive oxygen species (ROS)-responsive targeting nanoprodrug designed for selective therapy of cervical cancer. Such nanoprodrugs (CEC-OxbCD) are fabricated by the supramolecular self-assembly of the modified β-cyclodextrin (β-CD) and AMPs. Antimicrobial peptide, CecropinXJ (CEC), is a cationic antibacterial peptide isolated from 3rd instar larvae of Bombyx mori from Xinjiang, China. OxbCD is an oxidation-responsive β-cyclodextrin material. CEC-OxbCD were synthesized using the nanoprecipitation/self-assembly method. Subsequently, the particle size distribution, morphology, drug loading efficiency, and release behaviour of CEC-OxbCD were characterised. In vitro and in vivo anti-cancer activities were also evaluated. Nanoprodrugs can be effectively disassembled under stimuli of the tumor- endogenous ROS, resulting in a rapid and on-demand release of antimicrobial peptides (AMPs) with a release rate of 90 %. Furthermore, both in vitro and in vivo experimental results demonstrate that our nanoprodrugs exhibit remarkable therapeutic efficacy against cervical cancer. This work not only provides an effective and promising therapeutic strategy for cervical cancer, but also explores a novel application for AMPs.</div></div>","PeriodicalId":279,"journal":{"name":"Colloids and Surfaces B: Biointerfaces","volume":"247 ","pages":"Article 114411"},"PeriodicalIF":5.4,"publicationDate":"2024-11-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142754397","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Doped magnetic nanoparticles: From synthesis to applied technological frontiers

IF 5.4 2区医学

Colloids and Surfaces B: Biointerfaces Pub Date : 2024-11-26 DOI: 10.1016/j.colsurfb.2024.114410

Faizan ul Haq , Aasma Batool , Sobia Niazi , Imran Mahmood Khan , Ali Raza , Khubaib Ali , Junsong Yang , Zhouping Wang

{"title":"Doped magnetic nanoparticles: From synthesis to applied technological frontiers","authors":"Faizan ul Haq , Aasma Batool , Sobia Niazi , Imran Mahmood Khan , Ali Raza , Khubaib Ali , Junsong Yang , Zhouping Wang","doi":"10.1016/j.colsurfb.2024.114410","DOIUrl":"10.1016/j.colsurfb.2024.114410","url":null,"abstract":"<div><div>Doped magnetic nanoparticles (DMNPs) have become a fascinating class of nanomaterials with important implications in science and technology. The comprehensive review focuses on the synthetic methods, types of doping elements, distinctive properties, and extensive applications of DMNPs. The synthesis section highlights different methods, highlighting their benefits and drawbacks, such as chemical precipitation, co-precipitation, thermal breakdown, sol-gel, and other processes. Strategies for increasing the stability and functioning of DMNP are also reviewed, including surface functionalization and ligand exchange. An in-depth study is done to clarify how doping materials including transition metals, non-metals, and rare earth elements affect the chemical stability and magnetic characteristics of DMNP. Applications in various fields, such as biomedicine (MRI contrast agents, medication transport, antibacterial activity), environmental remediation (water purification, heavy metal removal), and sensing technologies, heavily rely on these features. DMNPs offer much potential in a variety of disciplines. Still, there are several challenges to their adoption, including regulatory and safety concerns, cost-effectiveness issues, and scalability issues. More research is required to overcome these difficulties and maximize the use of MDNPs for ensuring food safety.</div></div>","PeriodicalId":279,"journal":{"name":"Colloids and Surfaces B: Biointerfaces","volume":"247 ","pages":"Article 114410"},"PeriodicalIF":5.4,"publicationDate":"2024-11-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142757242","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Molecularly imprinted polymers (MIPs) for SARS-CoV-2 omicron variant inhibition: An alternative approach to address the challenge of emerging zoonoses

IF 5.4 2区医学

Colloids and Surfaces B: Biointerfaces Pub Date : 2024-11-26 DOI: 10.1016/j.colsurfb.2024.114408

Marco Dattilo , Francesco Patitucci , Marisa Francesca Motta , Sabrina Prete , Roberta Galeazzi , Silvia Franzè , Ida Perrotta , Mariangela Cavarelli , Ortensia Ilaria Parisi , Francesco Puoci

{"title":"Molecularly imprinted polymers (MIPs) for SARS-CoV-2 omicron variant inhibition: An alternative approach to address the challenge of emerging zoonoses","authors":"Marco Dattilo , Francesco Patitucci , Marisa Francesca Motta , Sabrina Prete , Roberta Galeazzi , Silvia Franzè , Ida Perrotta , Mariangela Cavarelli , Ortensia Ilaria Parisi , Francesco Puoci","doi":"10.1016/j.colsurfb.2024.114408","DOIUrl":"10.1016/j.colsurfb.2024.114408","url":null,"abstract":"<div><div>Emerging zoonoses pose significant public health risks and necessitate rapid and effective treatment responses. This study enhances the technology for preparing Molecularly Imprinted Polymers (MIPs), which function as synthetic nanoparticles targeting SARS-CoV-2 receptor-binding domain (RBD), specifically the Omicron variant, thereby inhibiting its function. This study builds on previous findings by introducing precise adjustments in the formulation and process conditions to enhance particle stability and ensure better control over size and distribution, thereby overcoming the issues identified in earlier research. Following docking studies, imprinted nanoparticles were synthesized via inverse microemulsion polymerization and characterized in terms of size, morphology and surface charge. The selective recognition properties and ability of MIPs to obstruct the interaction between ACE2 and the RBD of SARS-CoV-2 were assessed in vitro, using Non-Imprinted Polymers (NIPs) as controls, and rebinding studies were conducted utilizing a Quartz Crystal Microbalance with Dissipation monitoring (QCM-D). The synthesized nanoparticles exhibited uniform dispersion and had a consistent diameter within the nanoscale range. MIPs demonstrated significant recognition properties and exhibited a concentration-dependent ability to reduce RBD binding to ACE2 without cytotoxic or sensitizing effects. MIPs-based platforms offer a promising alternative to natural antibodies for treating SARS-CoV-2 infections, therefore representing a versatile platform for managing emerging zoonoses.</div></div>","PeriodicalId":279,"journal":{"name":"Colloids and Surfaces B: Biointerfaces","volume":"247 ","pages":"Article 114408"},"PeriodicalIF":5.4,"publicationDate":"2024-11-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142757238","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0