Self-pumping Janus nanofiber membrane with pH monitoring capability, integrated with a drug-loaded fast-dissolving layer for enhanced chronic wound healing

IF 5.6 2区医学 Q1 BIOPHYSICS

Colloids and Surfaces B: Biointerfaces Pub Date : 2025-09-04 DOI:10.1016/j.colsurfb.2025.115096

Jingjing Xi , Xiaoyan Qi , Yuxin Zhao , Yue Feng , Zhaolei Peng , Yulin Wang , Chunyan Cai , Dejun Yang , Liping Chen , Jia Luo , Xiaofang Li

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引用次数: 0

Abstract

The emergence of antimicrobial resistance poses significant challenges in conventional antibiotic treatments for chronic wound infections, highlighting an urgent need for alternative therapeutic strategies. To address this issue, we developed a multifunctional electrospun nanofiber dressing co-loaded with anthocyanin (ATH) and asiaticoside (AS) that possesses antimicrobial activity. The tri-layer dressing contains three functional components: a hydrophilic polyacrylonitrile-anthocyanin (PAN-ATH) layer for pH monitoring, a hydrophobic polycaprolactone (PCL) layer for exudate management, and a water-soluble pullulan-Bletilla striata polysaccharide-asiaticoside (PUL-BSP-AS) layer. Upon contact with wound exudate, the PUL-BSP-AS inner layer promptly dissolves to effectively suppress microbial growth during the early stage of wound healing. The exudate is then gradually absorbed through the hydrophobic PCL layer and transported to the hydrophilic PAN-ATH layer. The pH colorimetric analysis of Multilayer membrane established a precise exponential relationship between pH values and R/B as well as R/G ratios in the RGB color space. The Multilayer dressing can function as a biosensor to monitor wound pH levels and guide subsequent treatment. Antimicrobial assays demonstrated the antimicrobial activity (>88 %) of Multilayer membranes against Gram-positive (Staphylococcus aureus, Bacillus cereus), Gram-negative (Pseudomonas aeruginosa) bacteria, and the fungus (Candida albicans). Comprehensive in vitro and in vivo studies, combined with histopathological analysis, demonstrated that the dressing accelerates wound healing through multiple mechanisms, including antimicrobial, anti-inflammatory, and antioxidant effects, as well as upregulation of TGF-β and COL-1 expression to enhance collagen deposition. This innovative multilayer nanofiber system presents a promising therapeutic approach for chronic wound management with significant clinical potential.

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具有pH监测功能的自泵Janus纳米纤维膜，与药物负载的快速溶解层集成，可增强慢性伤口愈合

抗菌素耐药性的出现对慢性伤口感染的常规抗生素治疗提出了重大挑战，迫切需要替代治疗策略。为了解决这一问题，我们开发了一种具有抗菌活性的花青素（ATH）和积雪草苷（AS）共负载的多功能静电纺纳米纤维敷料。三层敷料包含三个功能成分：用于pH监测的亲水性聚丙烯腈-花青素（PAN-ATH）层，用于渗出物管理的疏水性聚已内酯（PCL）层和水溶性普鲁兰-白芨多糖-积雪草苷（pu - bsp -as）层。与创面渗出液接触后，pulp - bsp - as内层迅速溶解，有效抑制创面愈合初期微生物的生长。渗出液随后通过疏水性PCL层逐渐被吸收，并被输送到亲水性PAN-ATH层。通过对多层膜的pH比色分析，在RGB色彩空间中，pH值与R/B和R/G比值之间建立了精确的指数关系。多层敷料可以作为生物传感器监测伤口pH值并指导后续治疗。抗菌试验表明，多层膜对革兰氏阳性（金黄色葡萄球菌、蜡样芽孢杆菌）、革兰氏阴性（铜绿假单胞菌）细菌和真菌（白色念珠菌）具有抗菌活性（>88 %）。综合体外和体内研究，结合组织病理学分析表明，该敷料通过多种机制促进创面愈合，包括抗菌、抗炎、抗氧化作用，以及上调TGF-β和COL-1表达，促进胶原沉积。这种创新的多层纳米纤维系统为慢性伤口治疗提供了一种有前景的治疗方法，具有重要的临床潜力。

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来源期刊

Colloids and Surfaces B: Biointerfaces 生物-材料科学：生物材料

CiteScore

11.10

自引率

3.40%

发文量

730

审稿时长

42 days

期刊介绍： Colloids and Surfaces B: Biointerfaces is an international journal devoted to fundamental and applied research on colloid and interfacial phenomena in relation to systems of biological origin, having particular relevance to the medical, pharmaceutical, biotechnological, food and cosmetic fields. Submissions that: (1) deal solely with biological phenomena and do not describe the physico-chemical or colloid-chemical background and/or mechanism of the phenomena, and (2) deal solely with colloid/interfacial phenomena and do not have appropriate biological content or relevance, are outside the scope of the journal and will not be considered for publication. The journal publishes regular research papers, reviews, short communications and invited perspective articles, called BioInterface Perspectives. The BioInterface Perspective provide researchers the opportunity to review their own work, as well as provide insight into the work of others that inspired and influenced the author. Regular articles should have a maximum total length of 6,000 words. In addition, a (combined) maximum of 8 normal-sized figures and/or tables is allowed (so for instance 3 tables and 5 figures). For multiple-panel figures each set of two panels equates to one figure. Short communications should not exceed half of the above. It is required to give on the article cover page a short statistical summary of the article listing the total number of words and tables/figures.