Colloids and Surfaces B: Biointerfaces最新文献

{"title":"Enhancing radiosensitization in triple-negative breast cancer by aptamer-modified nanoclusters NC-T5-5TR1","authors":"Xuechun Kan ,&nbsp;Jifei Wang ,&nbsp;Yan Li ,&nbsp;Dongdong Li ,&nbsp;Fan Li ,&nbsp;Cheng Huang ,&nbsp;Peidang Liu","doi":"10.1016/j.colsurfb.2025.115397","DOIUrl":"10.1016/j.colsurfb.2025.115397","url":null,"abstract":"<div><div>Radiotherapy is an important part of standard treatment for triple-negative breast cancer (TNBC); however, radioresistance significantly limits therapeutic outcomes. In this study, we developed a novel radiosensitizer based on aptamer-modified silver nanoclusters, NC-T5–5TR1, to potentiate radiotherapy efficacy against TNBC. NC-T5–5TR1 enabled real-time tumor imaging and accurate localization via their intrinsic fluorescence and targeting capability. When combined with ionizing radiation (IR), NC-T5–5TR1 significantly enhanced radiosensitization <em>in vitro</em>, as evidenced by decreased proliferation, increased apoptosis and higher reactive oxygen species (ROS) levels, and also markedly inhibited tumor growth <em>in vivo</em>. Transcriptomic analysis indicated that the IL-6/JAK2/STAT3 signaling pathway was downregulated under NC-T5–5TR1 + IR treatment, which was subsequently validated <em>in vitro</em> and <em>in vivo</em>. Rescue experiments confirmed that IL-6 overexpression reversed NC-T5–5TR1 combined with IR-induced JAK2/STAT3 inhibition and its downstream effects on proliferation, apoptosis, and ROS accumulation. Collectively, our findings demonstrated that NC-T5–5TR1 enhanced the radiosensitivity of TNBC cells, and this effect was associated with the modulation of the IL-6/JAK2/STAT3 axis, offering a promising strategy for improving the therapeutic efficacy of radiotherapy in TNBC.</div></div>","PeriodicalId":279,"journal":{"name":"Colloids and Surfaces B: Biointerfaces","volume":"260 ","pages":"Article 115397"},"PeriodicalIF":5.6,"publicationDate":"2026-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145838043","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Polydopamine-functionalized PCN-224-supported Pt-Pd bimetallic nanozyme with intrinsic oxidase activity for hydrogen peroxide-free ultrasensitive colorimetric sensing of ascorbic acid","authors":"Huan Luo ,&nbsp;Shoubei Gao ,&nbsp;Chenran Zhen ,&nbsp;Haiyan Xiao ,&nbsp;Yanshuai Cui ,&nbsp;Longgang Wang","doi":"10.1016/j.colsurfb.2026.115413","DOIUrl":"10.1016/j.colsurfb.2026.115413","url":null,"abstract":"<div><div>Ascorbic acid (AA), an essential water-soluble antioxidant in humans, plays a critical role in disease prevention and health maintenance, necessitating accurate detection methods. However, conventional H₂O₂-dependent colorimetric methods face inherent limitations, creating a demand for alternative detection strategies that operate independently of H₂O₂. To address this problem, a composite nanozyme (PCN-224-PDA-PdPt₃) was constructed by employing a zirconium-based porphyrin metal-organic framework (PCN-224) as a substrate, followed by sequential polydopamine (PDA) surface functionalization and PdPt₃ bimetallic nanoparticle deposition. The synthetic nanozyme exhibited exceptional oxidase-mimicking activity at pH 4.0 and 40 °C. Mechanism studies confirmed that its activity originates from the catalytic generation of reactive oxygen species (O₂•⁻ and ¹O₂) from dissolved oxygen. Leveraging this, an H₂O₂-independent colorimetric AA detection method was developed, which demonstrated a wide linear range (1–900 μM), a low detection limit (0.56 μM), and high selectivity against interfering species like amino acids and metal ions. The clinical applicability was further verified through satisfactory recoveries (96.84–104.46 %) and low RSD (&lt; 5 %) in spiked human serum samples. Moreover, the nanozyme showed excellent biocompatibility with a hemolysis rate below 5 %. This work not only provides a robust and safe platform for biomedical detection but also paves the way for the rational design of multifunctional nanozymes for developing H₂O₂-independent sensing systems.</div></div>","PeriodicalId":279,"journal":{"name":"Colloids and Surfaces B: Biointerfaces","volume":"260 ","pages":"Article 115413"},"PeriodicalIF":5.6,"publicationDate":"2026-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145931740","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Corrigendum to “Polycaprolactone/polyvinylpyrrolidone coaxial electrospun fibers containing veratric acid-loaded chitosan nanoparticles for bone regeneration” [Colloids Surf. B Biointerfaces 193 (2020) 111110]","authors":"Sruthi Ranganathan,&nbsp;Kalimuthu Balagangadharan,&nbsp;Nagarajan Selvamurugan","doi":"10.1016/j.colsurfb.2026.115467","DOIUrl":"10.1016/j.colsurfb.2026.115467","url":null,"abstract":"","PeriodicalId":279,"journal":{"name":"Colloids and Surfaces B: Biointerfaces","volume":"260 ","pages":"Article 115467"},"PeriodicalIF":5.6,"publicationDate":"2026-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146045950","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Vibrio cholerae adhesin-derived peptides mediate strong pull-off forces in aqueous media with high ionic strength","authors":"Syeda Tajin Ahmed ,&nbsp;Sixin Zhai ,&nbsp;Xin Huang ,&nbsp;Sarvagya Sulaja ,&nbsp;Adekunle Adewole ,&nbsp;Alisa Ioffe ,&nbsp;Andrea D. Merg ,&nbsp;Jing Yan ,&nbsp;Roberto C. Andresen Eguiluz","doi":"10.1016/j.colsurfb.2025.115390","DOIUrl":"10.1016/j.colsurfb.2025.115390","url":null,"abstract":"<div><div>In this study, the pull-off forces of adsorbed films of four Bap1-inspired peptides in various solvents were investigated on negatively charged mica substrates using the surface forces apparatus (SFA), complemented with dynamic light scattering (DLS) for characterizing the aggregation behavior of peptides in solution. Bap1-inspired peptides consisted of the 57 amino acid wild-type sequence (WT); a scrambled version of the WT used to investigate the impact of the primary amino acid sequence in pull-off forces (Scr); a ten amino acid sequence rich in hydrophobic content (CP) of the WT sequence, and an eight amino acid sequence (Sh1) that corresponds to the pseudo-repeating sequence in the 57 AA. SFA results showed remarkable pull-off forces for CP, particularly in the presence of salts: measured pull-off forces were 26.0 ± 7.0 mN/m for no dwell-time and up to 42.0 ± 8.8 mN/m when surfaces were left in contact for 30 min. Sh1 did not bridge mica surfaces, while large aggregates clouded SFA measurements of Scr. DLS observations indicated that salts favored large peptide aggregation for all constructs (<em>D</em>_H &gt; 1 µm), as compared to milliQ (<em>D</em>_H ≈ 100–500 nm) water and DMSO (<em>D</em>_H ≈ 5 nm), resulting in heterogeneous peptide film thicknesses.</div></div>","PeriodicalId":279,"journal":{"name":"Colloids and Surfaces B: Biointerfaces","volume":"260 ","pages":"Article 115390"},"PeriodicalIF":5.6,"publicationDate":"2026-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145881606","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Biointerfacial mechanical responses of supported lipid membranes to Gramicidin D revealed by QCM-D","authors":"Şebnem Seherler,&nbsp;Abdulhalim Kılıç","doi":"10.1016/j.colsurfb.2025.115399","DOIUrl":"10.1016/j.colsurfb.2025.115399","url":null,"abstract":"<div><div>Gramicidin D (GrD) is a well-established model ion channel–forming peptide whose mechanical interactions with supported lipid membrane architectures remain less well characterized. Here, we combine overtone-resolved Quartz Crystal Microbalance with Dissipation (QCM-D), fluorescence microscopy, and solution-phase assays to examine how GrD perturbs supported lipid bilayers (SLBs) and supported vesicular layers (SVLs) formed from neutral DOPC and anionic DOPC:DOPS (92:08). On neutral SLBs at low peptide levels, GrD induces mild outer-leaflet softening consistent with β^6.3 channel formation, producing subtle acoustic signatures that evolve with slow kinetics. In contrast, neutral SVLs exhibit immediate hydration and thickening of the vesicle shell, reflected by large dissipation increases and overtone-dependent frequency shifts. Increasing peptide levels drive both platforms into a mechanically disruptive regime characterized by distinct overtone divergence and the formation of highly dissipative peptide–lipid assemblies, including rapid disruptive remodeling on SLBs and collapse of peptide-rich outer shells on SVLs. Membrane charge further modulates these responses. Anionic SLBs and SVLs confine GrD to shallow, leaflet-restricted perturbations near the headgroup region, yielding smaller overall mass changes but strong, overtone-specific viscoelastic heterogeneity. Solution-phase turbidity and proton permeability assay are consistent with functional channel activity dominating at lower peptide levels, whereas higher peptide levels align with non-conducting, mechanically disruptive interaction states. Together, these results show that GrD exhibits distinct, architecture-dependent mechanical interaction modes governed by peptide density, membrane geometry, and surface charge, and demonstrate how QCM-D measurements on complementary SLB and SVL platforms resolve nanoscale hydration and mechanical response modes that remain inaccessible to conventional ensemble assays.</div></div>","PeriodicalId":279,"journal":{"name":"Colloids and Surfaces B: Biointerfaces","volume":"260 ","pages":"Article 115399"},"PeriodicalIF":5.6,"publicationDate":"2026-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145881526","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The cinnamaldehyde-thiosemicarbazone-zinc (II) complex induces apoptosis in CAL-27 cells","authors":"Chen-yan Li ,&nbsp;Shuohua Xie ,&nbsp;Min Deng ,&nbsp;Ganrong Huang ,&nbsp;Yanqiang Huang ,&nbsp;Shufang Li","doi":"10.1016/j.colsurfb.2025.115374","DOIUrl":"10.1016/j.colsurfb.2025.115374","url":null,"abstract":"<div><h3>Objectives</h3><div>Oral squamous cell carcinoma (OSCC) is a major malignancy affecting the oral, jaw, and facial regions. In this study, we synthesized a cinnamaldehyde-thiosemicarbazone-zinc (II) complex (CTZn) to inhibit OSCC cell proliferation.</div></div><div><h3>Methods</h3><div>We investigated the inhibitory effects of CTZn on OSCC using the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay, cell colony formation assays, wound-healing/scratch assays and transwell invasion assays. Flow cytometry (FCM) was performed to explore the CTZn's impact on apoptosis and cell-cycle progression. Through <em>in vivo</em> experiments, its antitumor activity activity was evaluated in OSCC xenograft mouse models and orthotopic tumor model mice. The mechanisms of CTZn were explored by detecting targeted metabolites, and performing drug affinity responsive target stability (DARTS) experiments, molecular docking analyses and Western blot assays. Levels of reactive oxygen species (ROS), oxidized and reduced nicotinamide adenine dinucleotide phosphate (NADP⁺/NADPH), glutathione (GSH), and 6-phosphogluconate dehydrogenase (PGD) protein in CAL-27 cells were also determined.</div></div><div><h3>Results</h3><div>These findings demonstrated that CTZn inhibited CAL-27 cell growth <em>in vitro</em> in a time- and concentration-dependent manner, with an IC₅₀ value of 1.642–2.223μmol/L, and CTZn also exerted anti-tumor activity <em>in vivo</em>. CTZn also inhibited PGD messenger RNA (mRNA), and protein expression, reduced NADP+ /NADPH and GSH levels, and significantly increased ROS levels, thereby inducing oxidative stress.</div></div><div><h3>Conclusion</h3><div>CTZn impairs mitochondrial function, decreases ATP, levels, and induces G₂-phase arrest and apoptosis in CAL-27 cells. Therefore, it is an ideal drug for treating OSCC.</div></div>","PeriodicalId":279,"journal":{"name":"Colloids and Surfaces B: Biointerfaces","volume":"260 ","pages":"Article 115374"},"PeriodicalIF":5.6,"publicationDate":"2026-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145799403","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A novel macrophage membrane-camouflaged ultra-small copper sulfide and photothermal-responsive nitric oxide donor nanocomposites for enhanced synergistic antitumor therapy","authors":"Haoyang Gong ,&nbsp;Xiaonan Li ,&nbsp;Xinru Feng ,&nbsp;Dongjing Wang ,&nbsp;Xinyi Zhang ,&nbsp;Kai Wang ,&nbsp;Yanchen Liu ,&nbsp;Lu Xu ,&nbsp;Xueyan Zhou ,&nbsp;Yanzhuo Zhang","doi":"10.1016/j.colsurfb.2025.115373","DOIUrl":"10.1016/j.colsurfb.2025.115373","url":null,"abstract":"<div><div>Ultra-small copper sulfide (CuS) nanoparticles (NPs) possess exceptional photothermal performance, Fenton-like reaction activities, and multimodal imaging characteristics, while sodium nitroprusside serves as a clinical nitric oxide (NO) donor. A judicious combination of these two agents may facilitate the development of a novel multimodal synergistic antitumor strategy. In this context, we developed macrophage membrane-camouflaged and folic acid-conjugated nanocomposites (MF-SPC) that demonstrate dual responsiveness to near-infrared (NIR) laser and pH, aiming to enhance multimodal synergistic antitumor therapy. Within these biomimetic nanocomposites, sodium nitroprusside-doped Prussian blue NPs serve as the cores for inducing photothermal-responsive NO donation, while polydopamine layer embedded with CuS dots (sub-5 nm) act as the shells. This configuration not only enhances photothermal effects but also promotes Fenton-like reaction activity, glutathione depletion capabilities, and peroxidase activity. Furthermore, the dopamine groups on the shell surface facilitate folic acid modification, drug loading, and macrophage membrane camouflage. These functionalities collectively improve tumor-targeting abilities, promote immune evasion, and enhance tumor uptake. The doxorubicin-loaded MF-SPC (MDF-SPC) exhibited favorable dispersibility, stability, and pH-responsive sustained release properties. Both <em>in vivo</em> fluorescence imaging and NIR thermal imaging show that the MDF-SPC possessed active tumor-targeting capability. Thus, the MDF-SPC demonstrated high antitumor activity and biosafety when subjected to NIR laser irradiation in 4T1 tumor-bearing mice. Overall, the active tumor-targeting and retention capabilities, along with the rapid temperature elevations produced by PTT, allow the MDF-SPC to precisely and swiftly amplify chemodynamic therapy, gas therapy, and chemotherapy, providing a novel multimodal nanoplatform to promote antitumor therapy.</div></div>","PeriodicalId":279,"journal":{"name":"Colloids and Surfaces B: Biointerfaces","volume":"260 ","pages":"Article 115373"},"PeriodicalIF":5.6,"publicationDate":"2026-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145799404","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A user-friendly multifunctional hydrogel spray with adjustable mechanical properties for hemostasis and infected wound healing","authors":"Mengyao Gao ,&nbsp;Zongliang Wang ,&nbsp;Zhaohui Tang ,&nbsp;Peibiao Zhang","doi":"10.1016/j.colsurfb.2025.115378","DOIUrl":"10.1016/j.colsurfb.2025.115378","url":null,"abstract":"<div><div>Traumatic massive hemorrhage is a critical problem in the global emergency medicine field. Traditional hemostatic materials such as gauze and sponge have problems such as poor adhesion to irregular wounds and low adhesion strength. Existing hemostatic hydrogels also face bottlenecks such as insufficient active hemostatic efficiency, challenges in balancing interface adhesion and flexibility, and a lack of functional synergy, making it difficult to meet clinical needs. To address these challenges, this study develops a self-healing hydrogel spray that can form in situ for efficient hemostasis by simply mixing oxidized dextran (ODex) with polyethyleneimine (PEI) through a dynamic Schiff base reaction. Benefiting from the 'aldehyde-amino' active crosslinking mechanism, this hydrogel spray exhibits rapid gelation, triggering crosslinking within 15 s, with an adhesion strength exceeding 10 kPa, while also possessing good self-healing properties to adapt to dynamic and irregular wound. The electrostatic physical antibacterial properties of polyethyleneimine effectively suppress both Gram-positive and Gram-negative bacteria, avoiding the risk of drug resistance. By adjusting the oxidation degree of dextran, the hemostatic-adhesion synergy of the material has been further optimized, and the hydrogel preparation process is simple, cost-effective, user-friendly and can be stored at room temperature, with good biocompatibility. Animal experiments indicate that this hydrogel spray can quickly cover and adhere to the wound, effectively stopping bleeding, while reducing the risk of infection and promoting wound healing, providing innovative strategies and technical support for the development of a new generation of clinically available traumatic hemostatic materials.</div></div>","PeriodicalId":279,"journal":{"name":"Colloids and Surfaces B: Biointerfaces","volume":"260 ","pages":"Article 115378"},"PeriodicalIF":5.6,"publicationDate":"2026-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145825382","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Hydrogel composite nanomaterials for diabetic wound treatment","authors":"Nuonan Li ,&nbsp;Xianjun Luo ,&nbsp;Hongwen Zhu ,&nbsp;Guowang Shen ,&nbsp;Meifang Li ,&nbsp;Chunxia Zhou ,&nbsp;Ning Liang ,&nbsp;Puhua Zhang ,&nbsp;Xiaoyong Zhu","doi":"10.1016/j.colsurfb.2025.115392","DOIUrl":"10.1016/j.colsurfb.2025.115392","url":null,"abstract":"<div><div>Diabetes mellitus is slowly becoming more common over the past few years. One of the most disabling effects of diabetes is chronic skin ulcers. This is because certain materials have unique physicochemical properties that make them very useful for treating diabetic wounds. The three-dimensional network structure of hydrogels allows them to absorb large amounts of water. Their ability to bind water better, hold drugs effectively, and release drugs continuously is superior. Nanomaterials, particularly helpful in preventing bacterial infections and diabetic wounds caused by oxidative stress, are defined by at least one dimension measuring under 100 nanometers. Their unique qualities include antimicrobial action, facilitation of cell migration and proliferation, drug delivery systems, antioxidant and anti-inflammatory properties. This review delineates the properties and classifications of hydrogels, emphasizing their benefits as wound dressings. It then discusses the physicochemical properties of nanomaterials and their applications in wound healing. The main focus is on the basics of hydrogel-nanomaterial composites, their preparation techniques, and the synergistic benefits of their combination as wound dressings. The utilization of hydrogel nanocomposites in diabetic wound treatment is elucidated, and prospective research avenues are suggested to offer novel insights for clinical management.</div></div>","PeriodicalId":279,"journal":{"name":"Colloids and Surfaces B: Biointerfaces","volume":"260 ","pages":"Article 115392"},"PeriodicalIF":5.6,"publicationDate":"2026-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145831890","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Pullulan-based polymeric nanosystem for KEAP1 siRNA delivery: Mitigating ROS and promoting wound healing in vitro","authors":"Gülşen Bayrak,&nbsp;Dilara Karaguzel,&nbsp;Cagatay Karaaslan,&nbsp;Işık Perçin","doi":"10.1016/j.colsurfb.2025.115352","DOIUrl":"10.1016/j.colsurfb.2025.115352","url":null,"abstract":"<div><div>Recently, siRNA delivery has shown considerable potential for therapeutic use. While commercial options facilitate efficient delivery, they can be toxic to cells to a certain degree. Therefore, using relatively less toxic and safe delivery vehicles can increase the efficiency of therapy. This study examines the utilization of Pullulan–poly(ethyleneimine) nanoparticles (Pullulan-PEI-NPs) as an effective non-viral delivery system for Kelch-like ECH-associated protein 1 (KEAP1) siRNA, with the aims of mitigating oxidative stress and facilitating wound healing in HaCaT cells by enhancing cellular antioxidant mechanisms. The Pullulan-PEI-NPs were synthesized and characterized with Dynamic Light Scattering, Nanoparticle Tracking Analysis, Fourier Transform Infrared Spectroscopy, and electron microscopy techniques. The polyplexes were formed via electrostatic interaction between Pullulan-PEI-NPs and KEAP1 siRNA, and successful siRNA delivery was confirmed by cellular uptake analysis, mRNA expression studies, and KEAP1 and nuclear factor E2-related factor 2 (NRF2) protein level assessments. Pullulan-PEI-NPs had a silencing efficiency of 72 %, which is very close to the commercial transfection agent Lipofectamine 3000 (80 %). In following studies, KEAP1 siRNA delivery with Pullulan-PEI-NPs mitigates TBHP and H₂O₂ mediated ROS and facilitates wound healing with 98.9 % wound closure in a scratch assay on HaCaT cells. Obtained results highlight the therapeutic relevance of KEAP1 silencing in redox-regulated wound regeneration. This is the first report showing that Pullulan-PEI based cationic polymeric nanoparticles can be used to deliver KEAP1 siRNA and evaluate its role in mitigating ROS and increasing wound healing. These results highlight the promise of Pullulan-PEI-NPs as a safe and adaptable platform for non-viral gene therapy applications.</div></div>","PeriodicalId":279,"journal":{"name":"Colloids and Surfaces B: Biointerfaces","volume":"260 ","pages":"Article 115352"},"PeriodicalIF":5.6,"publicationDate":"2026-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145792840","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}