Colloids and Surfaces B: Biointerfaces最新文献

{"title":"Stimuli-responsive dual-drug loaded microspheres with differential drug release for antibacterial and wound repair promotion.","authors":"Yating Wu, Guihua Wei, Xin Cao, Ran Wang, Xue Gou","doi":"10.1016/j.colsurfb.2024.114455","DOIUrl":"https://doi.org/10.1016/j.colsurfb.2024.114455","url":null,"abstract":"<p><p>The healing of infected wounds is a complex and dynamic process requiring tailored treatment strategies that address both antimicrobial and reparative needs. Despite the development of numerous drugs, few approaches have been devised to optimize the timing of drug release for targeting distinct phases of infection control and tissue repair, limiting the overall treatment efficacy. Here, a stimuli-responsive microsphere encapsulating dual drugs was developed to facilitate differential drug release during distinct phases of antibacterial and repair promotion, thereby synergistically enhancing wound healing. Specifically, zeolite imidazolate backbone in poly (lactic-co-glycolic acid) (PLGA) microsphere was employed for the encapsulation of ciprofloxacin (CIP), responding to acidic environment of bacteria and releasing antibiotic for antibacterial therapy. Meanwhile, curcumin (CUR) encapsulated in PLGA exhibited a gradual release profile, contributing to synergistic antibacterial effects. During the tissue repair phase, near-infrared light stimulation of Fe₃O₄ embedded in PLGA generated heat, elevating the temperature to the glass transition point of PLGA, which significantly enhanced the release of CUR thereby promoting tissue repair. In vitro experiments demonstrated that the release of CIP and CUR achieved significant antibacterial effects in the early stages of treatment. Additionally, CUR could effectively enhance fibroblast migration and proliferation. In vivo studies using a mouse abscess model revealed that the microspheres exhibited remarkable antibacterial and wound-healing capabilities, effectively enhancing the re-epithelialization of wound tissue and reducing the infiltration of inflammatory cells. This study provides novel strategies for constructing drug delivery systems that match dynamic stages of wound healing, offering improved therapeutic outcomes for infected wounds.</p>","PeriodicalId":279,"journal":{"name":"Colloids and Surfaces B: Biointerfaces","volume":"248 ","pages":"114455"},"PeriodicalIF":5.4,"publicationDate":"2024-12-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142862588","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Exosomes derived from adipose mesenchymal stem cells promote corneal injury repair and inhibit the formation of scars by anti-apoptosis.","authors":"Chunli Ma, Yixiao Li, Baoling Liu, Junjie Deng, Xue Gao, Huixin Zhang, Bingqiang Zhang, Qihui Zhou, Xiaoting Peng, Han Zhang","doi":"10.1016/j.colsurfb.2024.114454","DOIUrl":"https://doi.org/10.1016/j.colsurfb.2024.114454","url":null,"abstract":"<p><p>In the corneal wound healing process, epithelial cell re-epithelialization and migration are the critical first steps following an injury. As the disease progresses, orderly regeneration of corneal stromal collagen and mild corneal stromal fibrosis are vital for corneal function reconstruction. Exosomes derived from adipose-derived mesenchymal stem cells (ADSCs-Exos) have emerged as a promising therapy due to their anti-oxidant, anti-apoptosis, and tissue repair properties. In this study, we successfully isolated exosomes via differential centrifugation and verified their effective extraction through transmission electron microscopy and nanoparticle tracking analysis. In vitro, ADSCs-Exos increased corneal epithelial cell migration by 20 % and reduced oxidative damage by 50 %. In addition, ADSCs-Exos demonstrated remarkable wound healing properties in corneal tissue. This effect was attributed to their ability to inhibit apoptosis of corneal stroma cells by upregulating Bax and downregulating Bcl2, reducing the Bax/Bcl2 protein expression ratio from 1 to 0.45. This decrease may subsequently inhibit α-SMA expression, thereby preventing corneal scarring. Overall, this research has elucidated the effects and potential targets of ADSCs-Exos in promoting corneal wound repair, offering a novel and promising approach for treating corneal injuries.</p>","PeriodicalId":279,"journal":{"name":"Colloids and Surfaces B: Biointerfaces","volume":"247 ","pages":"114454"},"PeriodicalIF":5.4,"publicationDate":"2024-12-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142826878","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Tumor-targeted nanosystem with hypoxia inducible factor 1α inhibition for synergistic chemo-photodynamic therapy against hypoxic tumor.","authors":"Ruifeng Zeng, Rui Zhou, Lu Zhen, Jinshuai Lan, Zhe Li, Donghao Gu, Wenlong Nie, Yi Shen, Minquan Zhang, Tong Zhang, Yue Ding","doi":"10.1016/j.colsurfb.2024.114456","DOIUrl":"https://doi.org/10.1016/j.colsurfb.2024.114456","url":null,"abstract":"<p><p>Photodynamic therapy (PDT) holds an essential role in the therapy of tumors. However, PDT consumes tissue oxygen and diminishes its own efficacy by inducing tumor hypoxia through the HIF-1α/VEGF pathway. Therefore, overcoming the photodynamic exacerbation of tumor hypoxia could reverse tumor microenvironment and enhance PDT. In this study, BC-PDA/HA loaded with bufalin (BUF) and chlorin e6 (Ce6) were developed for synergistic cancer chemo-photodynamic therapy. BC-PDA/HA, modified with hyaluronic acid (HA), exhibited CD44-targeting capability and enhanced cellular uptake in vitro. Moreover, in the acidic tumor microenvironment, BC-PDA/HA could on-demand release Ce6 and BUF, inducing PDT upon 660 nm irradiation. Simultaneously, the released BUF not only served as a chemotherapeutic agent, but also inhibited HIF-1α expression, reversing the PDT-induced tumor hypoxia. Furthermore, compared to free Ce6, BC-PDA/HA enhanced tumor accumulation and retention in vivo. BC-PDA/HA could also effectively improve hypoxia and inhibit tumor angiogenesis to enhance PDT efficacy, demonstrating synergistic chemo-PDT activity. In conclusion, this work provided a novel strategy for synergistic chemo-photodynamic therapy against breast cancer.</p>","PeriodicalId":279,"journal":{"name":"Colloids and Surfaces B: Biointerfaces","volume":"248 ","pages":"114456"},"PeriodicalIF":5.4,"publicationDate":"2024-12-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142890861","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Unilateral antibacterial Janus hydrogel hemostatic dressing prepared by the dragging effect of a brush.","authors":"Siqi Chen, Yanyan Zheng, Yan Fang, Yunxiang Weng, Haiqing Liu, Qinhui Chen","doi":"10.1016/j.colsurfb.2024.114453","DOIUrl":"https://doi.org/10.1016/j.colsurfb.2024.114453","url":null,"abstract":"<p><p>Hemostasis and subsequent anti-inflammatory measures are essential for wound healing in the human body following trauma or surgical procedures. Here, we try to use the dragging effect of a brush to prepare a Janus hydrogel with the least amount of bacteriostatic agent. The synthesized suspension of polyvinylbenzene-silica@quaternary ammonium salt (PDVB-SiO₂@NR₄⁺) Janus particles (JNPs) was selected as ink and brush coated onto one side of a polyacrylic acid (PAA) hydrogel, resulting in Janus hydrogel (JNPs≌PAA). The anisotropic chemical composition, wetting properties, adhesion capabilities, and the obtained hemostatic performance of the Janus hydrogel were studied thoroughly. The hydrophilic PAA side promoted tissue adhesion, while the hydrophobic JNPs side exhibited antibacterial effects. The Janus hydrogel presented perfect hemostatic effect in vivo, owing to the procoagulant effect of the adhesive layer, negative charges, and blood-blocking properties of the hydrophobic layer. The presence of quaternary ammonium groups in the Janus hydrogel imparted strong antibacterial activity against E. coli and S. aureus. Furthermore, the Janus hydrogel showed a low hemolysis rate and high cell safety. This multifunctional hydrogel material holds great promise for wound treatment and expands the applications of hydrogel materials in the biomedical field.</p>","PeriodicalId":279,"journal":{"name":"Colloids and Surfaces B: Biointerfaces","volume":"247 ","pages":"114453"},"PeriodicalIF":5.4,"publicationDate":"2024-12-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142826894","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Hydrogel-antimicrobial peptide association: A novel and promising strategy to combat resistant infections.","authors":"Marcelo Guerrero, David Filho, Nicolás Ayala A, Diana Rafael, Fernanda Andrade, Adolfo Marican, Sekar Vijayakumar, Esteban F Durán-Lara","doi":"10.1016/j.colsurfb.2024.114451","DOIUrl":"https://doi.org/10.1016/j.colsurfb.2024.114451","url":null,"abstract":"<p><p>Infections from multi-drug resistant bacteria (MDRB) have raised a worldwide concern, with projections indicating that fatalities from these infections could surpass those from cancer by 2050. This troubling trend is influenced by several factors, including the scarcity of new antibiotics to tackle challenging infections, the prohibitive costs of last-resort antibiotics, the inappropriate use of antimicrobial agents in agriculture and aquaculture, and the over-prescription of antibiotics in community settings. One promising alternative treatment is the application of antimicrobial peptides (AMPs) against MDRB. Hydrogels can facilitate the delivery of these antimicrobials, enhancing their biocompatibility and bioavailability. The Peptide-Hydrogel Association (PHA) capitalizes on the distinct properties of both peptides and hydrogels, resulting in multifunctional systems suitable for various antibacterial purposes. Multiple strategies can be employed to develop a PHA, including peptide-based hydrogels, hydrogels infused with peptides, and hydrogels modified with peptide functionalities. The research examined in this review showcases the strong effectiveness of these systems against MDRB and underscores their potential in creating multifunctional and multi-responsive solutions for various infection scenarios. The high efficacy of PHAs represents a promising and innovative therapeutic strategy in combating infections caused by MDRB.</p>","PeriodicalId":279,"journal":{"name":"Colloids and Surfaces B: Biointerfaces","volume":"247 ","pages":"114451"},"PeriodicalIF":5.4,"publicationDate":"2024-12-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142851832","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Ultrathin high-entropy hydrotalcites-based injectable hydrogel with programmed bactericidal and anti-inflammatory effects to accelerate drug-resistant bacterial infected wound healing.","authors":"Mingming Zhang, Huaping Jia, Liang Zhuang, Yongjie Xu, Ting Zhang, Jianwen Gu, Shan He, Dawei Li","doi":"10.1016/j.colsurfb.2024.114450","DOIUrl":"https://doi.org/10.1016/j.colsurfb.2024.114450","url":null,"abstract":"<p><p>Drug-resistant bacteria infected wounds often bring high risks of delayed healing process and even death. Sonodynamic therapy (SDT) can efficiently kill drug-resistant bacteria. However, superabundant reactive oxygen species (ROS) generated during SDT inevitably trigger significant inflammatory responses, hindering tissue remodeling. Herein, we develop intelligent ultrathin high-entropy hydrotalcites (UHE-HTs)-based injectable thermal-responsive hydrogel loaded with nicotinamide mononucleotide (UHE-HTs/PFN), aiming to achieve programmed antibacterial and anti-inflammatory effects. In the early infection stage, sonosensitive UHE-HTs/PFN hydrogel simultaneously can trigger rapid production of singlet oxygen (¹O₂) under ultrasound and efficient MDR bacterial sterilization. After halting ultrasonic irradiation, oxidoreductase-mimicking catalysis and nicotinamide mononucleotide release of UHE-HTs/PFN hydrogel effectively reduce ROS levels at wound sites, dampening the NF-κB inflammatory pathway. Such inhibited NF-κB expression can not only reduce the production of pro-inflammatory cytokines and inflammatory responses, but also significantly down-regulate the pyroptosis pathways (NLRP3/ASC/Casp-1) and inhibit pyroptosis that leads to inflammation. Moreover, significantly reduced ROS levels and synergistic release of Mg²⁺ reverse pro-inflammatory immune microenvironment. Both in vitro and in vivo assays demonstrate that UHE-HTs/PFN hydrogel can transform the adverse infected wound environment into a regenerative one by eradicating drug-resistant bacteria, scavenging ROS, and synergistic anti-inflammation. Therefore, this work develop an intelligent UHE-HTs/PFN hydrogel act as a \"lever\" that effectively achieve a balance between ROS generation and annihilation, rebuilding harmonious bactericidal and anti-inflammatory effects to remedy drug-resistant bacteria infected wound.</p>","PeriodicalId":279,"journal":{"name":"Colloids and Surfaces B: Biointerfaces","volume":"247 ","pages":"114450"},"PeriodicalIF":5.4,"publicationDate":"2024-12-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142821554","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The biological macromolecules constructed Matrigel for cultured organoids in biomedical and tissue engineering.","authors":"Ke-Yu Zhao, Yi-Xiang Du, Hui-Min Cao, Li-Ya Su, Xiu-Lan Su, Xian Li","doi":"10.1016/j.colsurfb.2024.114435","DOIUrl":"https://doi.org/10.1016/j.colsurfb.2024.114435","url":null,"abstract":"<p><p>Matrigel is the most commonly used matrix for 3D organoid cultures. Research on the biomaterial basis of Matrigel for organoid cultures is a highly challenging field. Currently, many studies focus on Matrigel-based biological macromolecules or combinations to construct natural Matrigel and synthetic hydrogel scaffolds based on collagen, peptides, polysaccharides, microbial transglutaminase, DNA supramolecules, and polymers for organoid culture. In this review, we discuss the limitations of both natural and synthetic Matrigel, and describe alternative scaffolds that have been employed for organoid cultures. The patient-derived organoids were constructed in different cancer types and limitations of animal-derived organoids based on the hydrogel or Matrigel. The constructed techniques utilizing 3D bioprinting platforms, air-liquid interface (ALI) culture, microfluidic culture, and organ-on-a-chip platform are summarized. Given the potential of organoids for a wide range of therapeutic, tissue engineering and pharmaceutical applications, it is indeed imperative to develop defined and customized hydrogels in addition to Matrigel.</p>","PeriodicalId":279,"journal":{"name":"Colloids and Surfaces B: Biointerfaces","volume":"247 ","pages":"114435"},"PeriodicalIF":5.4,"publicationDate":"2024-12-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142794143","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Curious effects of overlooked aspects on urease activity.","authors":"Mert Acar, Duccio Tatini, Valentina Romani, Barry W Ninham, Federico Rossi, Pierandrea Lo Nostro","doi":"10.1016/j.colsurfb.2024.114422","DOIUrl":"https://doi.org/10.1016/j.colsurfb.2024.114422","url":null,"abstract":"<p><p>Intermolecular forces determine complex chemical structures of exquisite intricacy, like proteins. However even the most advanced theories we have so far rely on too drastic approximations to explain them. Some crucial aspects that dictate structure, specific ion and solvent effects are not accommodated. Further the very significant effects of dissolved atmospheric gas are completely ignored and unexplored. Here we examine the effects of cations, dissolved gasses, and heavy water on the pH clock reactions of urease. This enzyme catalyzes the hydrolysis of urea to ammonium and bicarbonate in unbuffered aqueous solutions. In so doing it increases the pH. Circular dichroism and fluorescence experiments are used to assess conformational effects. The results highlight the subtle interplay of different factors that participate in determining the urease activity. The experimental data are correlated with specific ion physicochemical parameters and conformational data. They are explored in the context of specific ion and solvent interactions and hydration.</p>","PeriodicalId":279,"journal":{"name":"Colloids and Surfaces B: Biointerfaces","volume":"247 ","pages":"114422"},"PeriodicalIF":5.4,"publicationDate":"2024-12-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142823669","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Influence of agarose in semi-IPN hydrogels for sustained Polymyxin B release.","authors":"David Filho, Marcelo Guerrero, Ricardo Castro, Diana Rafael, Fernanda Andrade, Adolfo Marican, Oscar Valdes, Esteban Vargas, Elisa Valenzuela, Claudia Mora, Esteban F Durán-Lara","doi":"10.1016/j.colsurfb.2024.114431","DOIUrl":"https://doi.org/10.1016/j.colsurfb.2024.114431","url":null,"abstract":"<p><p>Hydrogels (HGs) are 3-D polymeric networks with high water content, making them appropriate for biomedical applications such as drug delivery systems. This study examines the impact of agarose in semi-interpenetrating polymer networks (Semi-IPNs) based on poly(acrylic acid) (p(AA)), N, N' Methylenebis(acrylamide) (MBA) and agarose (AGA) on the sustained release of Polymyxin B (PolB). Agarose incorporation improved the mechanical strength, swelling behavior and drug retention capacity of the HG. We synthesized the Semi-IPN HGs via free radical polymerization and characterized their structural and thermal properties using Fourier-transform infrared spectroscopy (FTIR), scanning electron microscopy (SEM), thermogravimetric analysis (TGA), and differential scanning calorimetry (DSC). The features of swelling under physiological conditions were carried out. Additionally, we conducted release kinetics using the three prepared HGs, each of which had a distinct amount of AGA. The findings demonstrated that the Semi-IPN HGs with greater AGA concentrations had drug release profiles that were slower and more sustained, making them perfect for long-term therapeutic uses. We also tested the PolB-loaded HGs' antimicrobial efficacy against Pseudomonas aeruginosa, and they showed sustained antibacterial activity. Using NIH-3T3 fibroblast cells, we verified the HGs' biocompatibility, demonstrating their appropriateness for use in biomedicine. According to these findings, agarose modified Semi-IPN HGs may find application in long-term medication delivery systems that aid in the treatment of infections and promote wound healing.</p>","PeriodicalId":279,"journal":{"name":"Colloids and Surfaces B: Biointerfaces","volume":"247 ","pages":"114431"},"PeriodicalIF":5.4,"publicationDate":"2024-12-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142823685","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Different mesoporous carbon carriers for the improvement of solubility and physical stability of poorly soluble drugs.","authors":"Wei Gu, Qinfu Zhao, Ye He, Shengyu Wang, Yuanqi Yang, Yian Li, Shuaipeng Feng, Siling Wang","doi":"10.1016/j.colsurfb.2024.114436","DOIUrl":"https://doi.org/10.1016/j.colsurfb.2024.114436","url":null,"abstract":"<p><p>Mesoporous carriers have gained significant attention for enhancing the solubility and bioavailability of Biopharmaceutics Classification System (BCS) Class II drugs. However, the contribution of mesoporous carriers with varying morphologies to the physical stability of these drugs is not well-defined. In this work, mesoporous carbon nanoparticles (MCN) and hollow carbon mesoporous nanoparticles (HMC) were prepared, while the weakly acidic Indomethacin (IMC) and alkaline Celecoxib (CXB) were incorporated into these carriers in the amorphous state by the solvent evaporation method. Notably, HMC demonstrated superior drug loading efficiencies (approximately 43 %) for both IMC and CXB owning to its hollow structure. The mesoporous drug loading systems significantly enhanced dissolution rates in comparison with both self-made amorphous drugs and raw drugs. Furthermore, under accelerated and long-term storage conditions, the mesoporous carriers effectively prevented drugs loaded from crystallization, maintaining constant dissolution profiles for over 12 months. Intriguingly, CXB exhibited a slower rate of crystallization after loading into the mesoporous carriers, likely due to the formation of hydrogen bonds between the carbonaceous carrier material and the amino groups of CXB. Compared with loaded drugs, the self-made amorphous drugs exhibited a crystallinity increase beyond 60 % within the initial month. Collectively, these findings highlighted the potential of mesoporous carbon carriers to elevate the dissolution behaviors of BCS Class II drugs while preserving the physical stability of the loaded amorphous drugs.</p>","PeriodicalId":279,"journal":{"name":"Colloids and Surfaces B: Biointerfaces","volume":"247 ","pages":"114436"},"PeriodicalIF":5.4,"publicationDate":"2024-12-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142870769","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}