Colloids and Surfaces B: Biointerfaces最新文献

{"title":"3D-bioprinting of MXenes: Developments, medical applications, challenges, and future roadmap","authors":"Muhammad Ikram ,&nbsp;M.A. Parvez Mahmud ,&nbsp;Amina Akbar Kalyar ,&nbsp;Thamer Alomayri ,&nbsp;Albandary Almahri ,&nbsp;Dilshad Hussain","doi":"10.1016/j.colsurfb.2025.114568","DOIUrl":"10.1016/j.colsurfb.2025.114568","url":null,"abstract":"<div><div>MXenes is a member of 2D transition metals carbides and nitrides with promising application prospects in energy storage, sensing, nanomedicine, tissue engineering, catalysis, and electronics. In the current era, MXenes have been widely applied in biomedical applications due to their unique rheological and electrochemical attributes. They have a larger surface area with more active sites, higher conductivity, lower cytotoxicity, and greater biocompatibility, making them highly suitable candidates for in-vivo biomedical applications. Due to recent advancemnets in MXenes 3D bioprinting, they are widely applied in regenerative medicine to combat challenges in suitable transplantation of tissues and organs. However, 3D bioprinting of MXenes has several complexities based on cell type, cytotoxicity, cell viability, and differentiation. To address these intricacies, surface modifications of MXene materials are done, which makes them highly fascinating for the 3D printing of tissues and organs. In the current review, we summarized recent progress in 3D bioprinting of MXene materials to construct scaffolds with desired rheological and biological properties, focusing on their potential applications in cancer phototherapy, tissue engineering, bone regeneration, and biosensing. We also discussed parameters affecting their biomedical applications and possible solutions by applying surface modifications. In addition, we addressed current challenges and future roadmaps for 3D bioprinting of MXene materials, such as generating high throughput 3D printed tissue constructs, drug delivery, drug discovery, and toxicology.</div></div>","PeriodicalId":279,"journal":{"name":"Colloids and Surfaces B: Biointerfaces","volume":"251 ","pages":"Article 114568"},"PeriodicalIF":5.4,"publicationDate":"2025-02-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143510439","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Inhalable lipid-based nanocarriers covered by polydopamine for effective mucus penetration and pulmonary retention","authors":"Xiaobo Zhang ,&nbsp;Zhengli Zhou ,&nbsp;Xiaohui Yang ,&nbsp;Lei Huang,&nbsp;Qin Wang,&nbsp;Yi Chen,&nbsp;Kesi Du,&nbsp;Jianqing Peng","doi":"10.1016/j.colsurfb.2025.114576","DOIUrl":"10.1016/j.colsurfb.2025.114576","url":null,"abstract":"<div><div>To overcome the critical challenge in drug inhalation for pulmonary diseases, we innovatively proposed that polydopamine (PDA) as a surface modification material had great potential to improve the mucus permeation and pulmonary retention of inhalable lipid-based nanocarriers. We prepared PDA coated lipid nanoemulsions/solid lipid nanoparticles/liposomes and systematically evaluated their interactions with mucin and pulmonary retention after inhalation. PDA-coated lipid-based nanocarriers exhibited weaker interactions with mucins, higher mucus permeability and cellular uptake by the respiratory epithelium cells compared to PEGylated lipid-based nanocarriers. However, the pulmonary retention advantage of PDA coating was shown in lipid nanoemulsions (&lt; 50 nm) and solid lipid nanoparticles (&lt; 100 nm). Liposomes (∼ 150 nm) with PEGylation possessed higher pulmonary retention than that coated by PDA. It was suggested that PEGylated liposomes were liable to be phagocytosed by alveolar macrophages due to binding with specific antibodies. Overall, this work suggests that PDA as a surface modification material of inhalable lipid-based nanocarriers holds promise for effective mucus penetration and pulmonary retention.</div></div>","PeriodicalId":279,"journal":{"name":"Colloids and Surfaces B: Biointerfaces","volume":"251 ","pages":"Article 114576"},"PeriodicalIF":5.4,"publicationDate":"2025-02-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143465486","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A novel NIR-dependent IDO-inhibiting ethosomes treatment melanoma through PTT/PDT/immunotherapy synergy","authors":"Changzhen Sun ,&nbsp;Qingqing He ,&nbsp;Xun Yang ,&nbsp;Jianv Wang ,&nbsp;Dengmei Xia ,&nbsp;Tong Xia ,&nbsp;Hongye Liao ,&nbsp;Xia Xiong ,&nbsp;Yongmei Liao ,&nbsp;Hongping Shen ,&nbsp;Qin Sun ,&nbsp;Yuan Yuan ,&nbsp;Yuanmin He ,&nbsp;Li Liu","doi":"10.1016/j.colsurfb.2025.114565","DOIUrl":"10.1016/j.colsurfb.2025.114565","url":null,"abstract":"<div><div>Phototherapy is a treatment method that uses the characteristics of different bands of light to treat diseases. Tumor immunotherapy, on the other hand, treats tumors by regulating the body’s immune system. The combination of phototherapy and immunotherapy can significantly enhance the treatment of melanoma. In this study, we prepared and characterized INEs, a novel ethosome composed of the photosensitizer IR251 and the Indoleamine-2, 3-dioxygenase (IDO) inhibitor NLG919. INEs demonstrated excellent phototherapeutic properties, strong phototoxicity, and a notable ability to inhibit IDO. Under 808 nm laser irradiation, INEs effectively induced immunogenic cell death (ICD) in melanoma cells. <em>In vivo</em> experiments demonstrated that INEs injection into primary tumors triggered ICD, promoted maturation of DC cells, and activated naive T cells, leading to the production of effector T cells (specifically CD4⁺ and CD8⁺ T cells) that targeted and killed tumor cells. Both primary and distant tumors were treated simultaneously with favorable biosafety. In conclusion, INEs represent a promising strategy for melanoma treatment by a combination of phototherapy and immunotherapy with high safety. This study provides new insights and a theoretical basis for the clinical treatment of melanoma.</div></div>","PeriodicalId":279,"journal":{"name":"Colloids and Surfaces B: Biointerfaces","volume":"251 ","pages":"Article 114565"},"PeriodicalIF":5.4,"publicationDate":"2025-02-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143473969","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Hydrogels of diet-derived electron donors restore epithelial hypoxia and reduce iNOS synthesis to inhibit inflammation-induced overgrowth of facultatively anaerobic bacteria for gut homeostasis","authors":"Siying Cheng ,&nbsp;Hongliang Wang ,&nbsp;Xiaoqian He ,&nbsp;Yun Shao ,&nbsp;Fengguang Ma ,&nbsp;Jianan Huang ,&nbsp;Bing Hu ,&nbsp;Zhonghua Liu","doi":"10.1016/j.colsurfb.2025.114574","DOIUrl":"10.1016/j.colsurfb.2025.114574","url":null,"abstract":"<div><div>Food hydrogels targeting respiration of microorganisms via changing the micro-ecological environment in gut were prepared through the self-assembly of polyphenols extracted from tea leaves harvested in summer and autumn and the protein fibrils originating from egg white lysozyme. Oral administration with the hydrogels effectively inhibited the over-expansion of the facultative anaerobic bacterium indicated by <em>E. coli</em> Nissle 1917 (EcN) and alleviated the clinic symptoms of chronic intestinal inflammation in mice. Importantly, the hypoxia of epithelial cells was elevated significantly and the overexpression of the inducible NO synthase (INOs)-related NOS2 gene was inhibited substantially in colons of the colitis mice, which accounted for prevention of the abnormal expansion of <em>E. coli via</em> blocking respiration. The treatment with the hydrogels preserved normal mitochondrial function in colonic epithelial cells under oxidative stress, which could serve as the mechanism to maintain the capability to consume oxygen.</div></div>","PeriodicalId":279,"journal":{"name":"Colloids and Surfaces B: Biointerfaces","volume":"250 ","pages":"Article 114574"},"PeriodicalIF":5.4,"publicationDate":"2025-02-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143454448","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Construction of a methacrylated gelatine composite hydrogel based on tachyplesin II and its application in the repair of infected wounds","authors":"Jingna Wu ,&nbsp;Bangfeng Lin ,&nbsp;Nan Pan ,&nbsp;Xiaoting Chen ,&nbsp;Bei Chen ,&nbsp;Mingming Hou ,&nbsp;Xiaoya Qu","doi":"10.1016/j.colsurfb.2025.114575","DOIUrl":"10.1016/j.colsurfb.2025.114575","url":null,"abstract":"<div><div>Tachyplesin II (TP) is a β-folded peptide isolated from horseshoe crabs. TP, with two disulfide bonds, effectively inhibits gram-negative and gram-positive bacteria, fungi and viruses. In this study, the antibacterial and antioxidant properties of synthetic TP were examined. Specifically, TP was incorporated into cross-linked methacrylated gelatine (GelMA) to construct a GelMA-TP hydrogel, which promoted infected wound healing. TP formed intermolecular hydrogen bonds with the GelMA network, endowing the GelMA-TP hydrogel with good antibacterial activity, stable rheological properties, high swelling capacity, self-healing behaviour, and good biocompatibility. We found that the anti-inflammatory and antioxidant activities of the GelMA-TP hydrogel significantly enhanced collagen production, thus accelerating healing in a rat-infected wound model. Overall, the GelMA-TP hydrogel demonstrates significant potential to stimulate the healing of infected wounds by reducing healing time and improving tissue repair outcomes. These findings highlight its translational promise as a clinically effective material for managing complex wounds, particularly in scenarios where conventional therapies are limited by persistent infections or excessive inflammation.</div></div>","PeriodicalId":279,"journal":{"name":"Colloids and Surfaces B: Biointerfaces","volume":"250 ","pages":"Article 114575"},"PeriodicalIF":5.4,"publicationDate":"2025-02-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143454447","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Advances in nanoplatform-based multimodal combination therapy activating STING pathway for enhanced anti-tumor immunotherapy","authors":"Huizhong Zhang,&nbsp;Xiaohan Xu,&nbsp;Shiman Li,&nbsp;Huating Huang,&nbsp;Ke Zhang,&nbsp;Wenjing Li,&nbsp;Xinzhu Wang,&nbsp;Jingwen Yang,&nbsp;Xingbin Yin,&nbsp;Changhai Qu,&nbsp;Jian Ni,&nbsp;Xiaoxv Dong","doi":"10.1016/j.colsurfb.2025.114573","DOIUrl":"10.1016/j.colsurfb.2025.114573","url":null,"abstract":"<div><div>Activation of the cyclic GMP-AMP synthase(cGAS)-stimulator of interferon genes (STING) has great potential to promote antitumor immunity. As a major effector of the cell to sense and respond to the aberrant presence of cytoplasmic double-stranded DNA (dsDNA), inducing the expression and secretion of type I interferons (IFN) and STING, cGAS-STING signaling pathway establishes an effective natural immune response, which is one of the fundamental mechanisms of host defense in organisms. In addition to the release of heterologous DNA due to pathogen invasion and replication, mitochondrial damage and massive cell death can also cause abnormal leakage of the body's own dsDNA, which is then recognized by the DNA receptor cGAS and activates the cGAS-STING signaling pathway. However, small molecule STING agonists suffer from rapid excretion, low bioavailability, non-specificity and adverse effects, which limits their therapeutic efficacy and in vivo application. Various types of nano-delivery systems, on the other hand, make use of the different unique structures and surface modifications of nanoparticles to circumvent the defects of small molecule STING agonists such as fast metabolism and low bioavailability. Also, the nanoparticles are precisely directed to the focal site, with their own appropriate particle size combined with the characteristics of passive or active targeting. Herein, combined with the cGAS-STING pathway to activate the immune system and kill tumor tissues directly or indirectly, which help maximize the use of the functions of chemotherapy, photothermal therapy(PTT), chemodynamic therapy(CDT), and radiotherapy(RT). In this review, we will discuss the mechanism of action of the cGAS-STING pathway and introduce nanoparticle-mediated tumor combination therapy based on the STING pathway. Collectively, the effective multimodal nanoplatform, which can activate cGAS-STING pathway for enhanced anti-tumor immunotherapy, has promising avenue clinical applications for cancer treatment.</div></div>","PeriodicalId":279,"journal":{"name":"Colloids and Surfaces B: Biointerfaces","volume":"250 ","pages":"Article 114573"},"PeriodicalIF":5.4,"publicationDate":"2025-02-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143444474","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Tannic acid coating modification of polypropylene providing pH-responsive antibacterial and anti-inflammatory properties applicable to ostomy patches","authors":"Qifen Li ,&nbsp;Leixiang Wang ,&nbsp;Yuxin Li ,&nbsp;Shukai Nan ,&nbsp;Ziqing Tan ,&nbsp;Qian Yang ,&nbsp;Chengbo Li ,&nbsp;Xianrui Xie ,&nbsp;Huanhuan Yan ,&nbsp;Guige Hou ,&nbsp;Shun Duan ,&nbsp;Yu-Qing Zhao","doi":"10.1016/j.colsurfb.2025.114567","DOIUrl":"10.1016/j.colsurfb.2025.114567","url":null,"abstract":"<div><div>We report highly efficient, antioxidant, anti-inflammatory, pH-responsive antibacterial coatings developed via direct assembly of a nature compound tannic acid (TA) with the cationic antibiotic quaternized polyethyleneimine (QPEI). The surface of polypropylene was modified with these coatings. Under acidic conditions, the coatings significantly enhanced the antibacterial performance against <em>Staphylococcus aureus</em> and <em>Escherichia coli,</em> and the antibacterial rate reached more than 90 %. The free radical scavenging rate could exceed 91 %. Thus, the excess reactive oxygen species (ROS) could be cleared, and the oxidative stress production could be significantly reduced. <em>In vitro</em> anti-inflammatory experiments revealed that the coatings significantly reduced the expression of TNF-α and IL-6 and promoted the release of IL-10. These results indicated the excellent anti-inflammatory effects of the coatings. <em>In vivo</em> experiments revealed that the coatings could rapidly achieve bactericidal effects and subsequently prevent inflammatory reactions, thereby inhibiting the generation of fibrosis. Through molecular docking simulation experiments, the mechanism of LBL self-assembly between QPEI and TA components has been clarified for the first time. By designing the surface coating of a material and combining it with bioactive components, multiple functions could be achieved to meet the clinical needs of stoma patches and promote the development of medical materials.</div></div>","PeriodicalId":279,"journal":{"name":"Colloids and Surfaces B: Biointerfaces","volume":"250 ","pages":"Article 114567"},"PeriodicalIF":5.4,"publicationDate":"2025-02-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143444470","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Dissolving microneedles: Drug delivery and disease treatment","authors":"Yanling Zhuo ,&nbsp;Fangyue Wang ,&nbsp;Qizhuang Lv ,&nbsp;Chunyan Fang","doi":"10.1016/j.colsurfb.2025.114571","DOIUrl":"10.1016/j.colsurfb.2025.114571","url":null,"abstract":"<div><div>Traditional transdermal drug delivery methods are often plagued by technical inefficiencies, limited absorption, and the potential for adverse reactions. In contrast, dissolving microneedles (DMNs) offer a novel approach to transdermal drug delivery by effectively merging the benefits of subcutaneous injection with those of conventional transdermal methods. These microneedles dissolve completely within the body, releasing the encapsulated antigen without leaving any sharp remnants. Furthermore, DMNs overcome the limitations of traditional transdermal patches, which are restricted to delivering only small molecule drugs. By facilitating the efficient transdermal absorption of large molecules, DMNs enable precise and painless disease treatment. With advantages such as effective delivery, safety, controllable administration, DMNs hold significant promise in the fields of disease treatment and drug delivery. This article explores the substrate materials, preparation techniques, characterization methods, and current applications of DMNs. We also discuss the current challenges and obstacles faced by DMNs. Finally, we outline potential future research directions for DMNs, aiming to provide a theoretical reference for researchers involved in their preparation and application.</div></div>","PeriodicalId":279,"journal":{"name":"Colloids and Surfaces B: Biointerfaces","volume":"250 ","pages":"Article 114571"},"PeriodicalIF":5.4,"publicationDate":"2025-02-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143444472","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Polycaprolactone (PCL)‐Gelatin Electrospun Meshes for Accelerated Gastric Wound Healing","authors":"Sara F.C. Guerreiro ,&nbsp;Filipa Leal ,&nbsp;Anabela G. Dias ,&nbsp;Pedro L. Granja ,&nbsp;Juliana R. Dias","doi":"10.1016/j.colsurfb.2025.114572","DOIUrl":"10.1016/j.colsurfb.2025.114572","url":null,"abstract":"<div><div>Gastric wound healing constitutes a complex challenge, even in the context of superficial wounds, due to the harsh stomach environment, characterized by high pH variability and dynamic enzyme concentrations. Therefore, effective healing depends on robust mechanical support and adequate biochemical cues to drive cell growth and proliferation. Electrospun-based dressings may offer a solution to these problems by providing physical support that mimics native extracellular matrix. In this study, electrospun dressings composed of a blend of polycaprolactone (PCL) and gelatin (Gel) were proposed for the first time for gastric application by tuning the relative PCL:Gel ratios (75:25, 50:50 and 25:75) to optimize both their retention capacity and cellular interaction. PCL/Gel dressings, in a proportion of 75:25, showed to have efficient mucoadhesion (ultimate stress of 1.8 MPa) when tested in <em>ex vivo</em> porcine samples. They were also stable in simulated gastric fluid for 14 days, a period compatible with the treatment window. Moreover, the non-cytotoxic biological response (&gt;90 %) of the dressings was favorably validated in mouse fibroblast L929 cell line. Cell morphology, metabolic activity, cell viability and proliferative capacity were assessed using human specific gastric cell lines, including normal stomach fibroblasts (NST-20) and gastric adenocarcinoma (AGS). Overall, PCL/Gel dressings of 75:25 increased the proliferation rate of NST20 and AGS cells after 3 and 7 days in culture, respectively, with significant expression of proliferation marker Ki-67 protein.</div></div>","PeriodicalId":279,"journal":{"name":"Colloids and Surfaces B: Biointerfaces","volume":"250 ","pages":"Article 114572"},"PeriodicalIF":5.4,"publicationDate":"2025-02-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143471310","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Collagen-mediated in situ mineralization-enhanced biomimetic bone tissue engineering scaffolds","authors":"Rui Shi ,&nbsp;Xiaotong Wang ,&nbsp;Fangli Gang ,&nbsp;Jiayu Shi ,&nbsp;Shuping Wang ,&nbsp;Wanting Liu ,&nbsp;Weilong Ye ,&nbsp;Xiaodan Sun","doi":"10.1016/j.colsurfb.2025.114566","DOIUrl":"10.1016/j.colsurfb.2025.114566","url":null,"abstract":"<div><div>Oriented poly(lactic acid) (PLA) fiber bone tissue engineering scaffolds are often limited by factors including poor material hydrophilicity and weak osteogenic activity. The introduction of in situ mineralization can address these issues, but it requires the assistance of hydrophilic materials to achieve optimal performance. Collagen, a nature-based ECM component, was adopted because it can enhance hydrophilicity, encourage cell adhesion, and biomimetrically induce mineralization, according to recent studies of ECM-mimicking scaffolds. Therefore, this study proposes a collagen-mediated in situ mineralization-enhanced scaffold design aimed at improving the hydrophilicity and osteogenic potential of oriented fiber scaffolds. Collagen (5–10 wt%) and phosphate-containing solutions (59.6 mM) were added to a PLA matrix, and scaffolds were electrospun at 12 kV. Subsequently, the scaffolds underwent in situ mineralization in a calcium ion-containing solution (101 mM), leading to the formation of calcium phosphate within the scaffold structure. The experimental results show that the introduction of collagen effectively promoted the formation of in situ mineralization, enhanced the hydrophilicity of the scaffold, and maintained good fiber orientation. The scaffolds exhibited significant mechanical anisotropy, with the Young's modulus parallel to the fiber direction reaching 5 MPa, which is 25 times greater than that in the direction perpendicular to the fibers. In vitro studies with rat bone marrow mesenchymal stem cells showed a 2.4-fold increase in osteogenic differentiation, as assessed by alkaline phosphatase activity. Micro-CT analysis showed that the increase of BV/TV was 3.26 times higher when compared to that of control scaffolds, while histological analysis revealed mature bone tissue formation characterized by well-organized collagen fibers. Overall, the present study describes a novel strategy of collagen-mediated in situ mineralization, first integrating enhanced hydrophilicity, mechanical anisotropy, and biomimetic bone-like properties to address major limitations associated with the current oriented fiber scaffolds.</div></div>","PeriodicalId":279,"journal":{"name":"Colloids and Surfaces B: Biointerfaces","volume":"250 ","pages":"Article 114566"},"PeriodicalIF":5.4,"publicationDate":"2025-02-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143429619","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}