Colloids and Surfaces B: Biointerfaces最新文献

{"title":"TPP-alantolactone conjugates and their nanotherapeutic forms for antitumor application","authors":"Andrey V. Nemtarev ,&nbsp;Mariya E. Shemakhina ,&nbsp;Tatiana N. Pashirova ,&nbsp;Alexandra D. Voloshina ,&nbsp;Eliana B. Souto ,&nbsp;Anna P. Lyubina ,&nbsp;Syumbelya K. Amerkhanova ,&nbsp;Leysan R. Idrisova ,&nbsp;Alexey V. Semakov ,&nbsp;Vladimir F. Mironov","doi":"10.1016/j.colsurfb.2025.115127","DOIUrl":"10.1016/j.colsurfb.2025.115127","url":null,"abstract":"<div><div>Delocalized positive charge and high hydrophobicity of triarylphosphonium (TPP) groups facilitate a penetration of the TPP-conjugated molecules through the cell and mitochondrial membranes, which result in their accumulating in tumor cells. Combining mitochondrial strategies with nanotechnology-based delivery systems is a challenge to the new cancer therapy approaches. Using synthetic approach for targeted delivery of small molecules with antitumor activity into mitochondria, the quaternary γ-oxoalkylphosphonium salts (TPP–alantolactone, TPP–AL) were synthesized under mild conditions with high yields. First time mitochondria-targeted lipid nanosystems, namely, liposomes and solid lipid nanoparticles (SLN) modified with TPP–AL were prepared and characterized. TPP–alantolactones showed a high <em>in vitro</em> cytotoxicity. The highest cytotoxicity against human duodenal adenocarcinoma cell lines (HuTu 80) occurred at IC₅₀ = 0.4 µM with a high selectivity of 17.5. Cytotoxicity is increased up to 520-fold when tested TPP–alantolactone-SLN (TPP–AL–SLN) against M-HeLa cancer cell lines. The enhanced cellular uptake and accumulation of curcumin-labeled-TPP–AL–SLN were shown by confocal microscopy. TPP–AL caused a significant depolarization of mitochondrial membrane in the HuTu 80 cancer cells, increased ROS production and over expression of caspase-9, suggesting an intrinsic mitochondrial mechanism for triggering apoptosis. A significant delay of cells in the G0/G1 phase compared to the control was revealed. In addition to the anti-tumor effect TPP–AL exhibit a bactericidal activity, i.e. they are dual-action drugs. TPP–AL are noticeably active against gram-positive bacteria, including methicillin-resistant <em>Staphylococcus aureus</em> (MRSA), with a minimum bactericidal concentration (MBC) of 7.8 µM, which is close to the antibiotic norfloxacin. TPP-derived sesquiterpene lactones-decorated nanosystems showed a high potential as antitumor agents for the targeted delivery to the mitochondria of tumor cells.</div></div>","PeriodicalId":279,"journal":{"name":"Colloids and Surfaces B: Biointerfaces","volume":"257 ","pages":"Article 115127"},"PeriodicalIF":5.6,"publicationDate":"2025-09-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145046275","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Corrigendum to \"Robotic manipulations of single cells using a large-volume piezoelectric micropipette with nanoliter precision\" [Colloid. Surf. B Biointerfaces 256 (2025) 114972].","authors":"Boglarka Kovacs, Szabolcs Novak, Igor Sallai, Beatrix Magyarodi, Inna Szekacs, David Selmeczi, Balint Szabo, Robert Horvath","doi":"10.1016/j.colsurfb.2025.115099","DOIUrl":"10.1016/j.colsurfb.2025.115099","url":null,"abstract":"","PeriodicalId":279,"journal":{"name":"Colloids and Surfaces B: Biointerfaces","volume":" ","pages":"115099"},"PeriodicalIF":5.6,"publicationDate":"2025-09-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145028646","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Highly sensitive sunset yellow-based optical and dielectric biosensing of SARS-CoV-2 nucleocapsid protein in saliva","authors":"Mon-Juan Lee ,&nbsp;Jui-Teng Hsieh ,&nbsp;Chia-Tung Chang ,&nbsp;Yi-Chong Wu ,&nbsp;Po-Yu Chan ,&nbsp;Ching‑Ming Chien ,&nbsp;Wei Lee","doi":"10.1016/j.colsurfb.2025.115128","DOIUrl":"10.1016/j.colsurfb.2025.115128","url":null,"abstract":"<div><div>Optical and dielectric immunosensors for the nucleocapsid (N) protein of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) were developed using the nematic phase of a lyotropic chromonic liquid crystal (LCLC), sunset yellow (SSY), as the sensing medium. The biosensing platform was constructed as a liquid crystal (LC) cell with a cell gap of 15 <em>μ</em>m coated with a homeotropic alignment reagent so that nematic SSY, a 28 wt% aqueous solution of SSY sandwiched between the parallel glass slides of a LC cell, was aligned homeotropically. In the presence of a controlled amount of immobilized anti-N protein antibody, nematic SSY remained homeotropically aligned, yielding a dark optical texture under a polarizing optical microscope with crossed polarizers. In the presence of the analyte, the formation of N protein immunocomplexes disrupted the ordered orientation of nematic SSY, leading to light leakage and a change in both the real and imaginary parts of the dielectric constant, which correlated with N protein concentration, with the limit of detection (LOD) comparable or superior to most electrochemical and label-based SARS-CoV-2 N protein biosensors. Moreover, the hydrophilic nature of LCLC enabled N protein to be solubilized in nematic SSY so that kinetic analysis of specific binding between the antigen and antibody can be performed, which is unachievable with conventional thermotropic liquid crystal-based biosensors. Finally, as SARS-CoV-2 N protein can be detected noninvasively in human saliva, the clinical potential of the nematic SSY-based immunoassay was demonstrated using artificial saliva spiked with N protein.</div></div>","PeriodicalId":279,"journal":{"name":"Colloids and Surfaces B: Biointerfaces","volume":"257 ","pages":"Article 115128"},"PeriodicalIF":5.6,"publicationDate":"2025-09-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145046267","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Electrospun porous nanofibers for sustained drug delivery: Degradation-controlled release through architectural design","authors":"Min Lao,&nbsp;Xin Li,&nbsp;Yingjie Wang,&nbsp;Junlang Li,&nbsp;Zhengjie Tian,&nbsp;Jiale Zhang,&nbsp;Shaofeng Yin,&nbsp;Xiaoting Deng","doi":"10.1016/j.colsurfb.2025.115126","DOIUrl":"10.1016/j.colsurfb.2025.115126","url":null,"abstract":"<div><div>Diclofenac sodium (DS), a non-steroidal anti-inflammatory drug used for treating inflammatory pain, has a short elimination half-life, which can lead to fluctuations in blood drug concentration. Therefore, developing sustained-release formulations is necessary to meet clinical needs. Biodegradable polymers exhibit excellent sustained-release properties and good biocompatibility, making them suitable for processing into nanofiber-based drug delivery systems via electrospinning technology. Using electrospinning combined with a non-solvent-induced phase separation mechanism, porous nanofibers with different structures were successfully prepared, including non-porous uniaxial nanofibers, porous coaxial nanofibers, double-layered nanofibers with a porous mesh surface, and porous uniaxial nanofibers. The results demonstrated that drug release is influenced by fiber structure and morphology. Among these, the coaxial porous shell-core structure(NFs 2) achieved long-term release kinetics, confirming a synergistic mechanism combining diffusion and matrix degradation. The prepared samples were analyzed using kinetic modeling and were found to conform to the Ritger-Peppas model. This study investigates the impact of electrospun nanofiber structure and morphology on drug delivery, providing significant theoretical and practical insights for the development of innovative sustained-release formulations.</div></div>","PeriodicalId":279,"journal":{"name":"Colloids and Surfaces B: Biointerfaces","volume":"257 ","pages":"Article 115126"},"PeriodicalIF":5.6,"publicationDate":"2025-09-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145004708","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"An injectable hyaluronic acid-based hydrogel for the treatment of breast cancer","authors":"Xin Shen ,&nbsp;Yang Li ,&nbsp;Xinru Kang ,&nbsp;Chenhui Zhu ,&nbsp;Zhiguang Duan ,&nbsp;Rongzhan Fu","doi":"10.1016/j.colsurfb.2025.115100","DOIUrl":"10.1016/j.colsurfb.2025.115100","url":null,"abstract":"<div><div>In this study, we develop a hyaluronic-tannic acid (HA-TA) hydrogel loaded with Cu nanoparticles attach to MXene (MXene@Cu) to explore its potential as a targeted breast cancer treatment. The MXene@Cu nanosheets exhibit activity in depleting glutathione (GSH) and inducing reactive oxygen species (ROS) through the Fenton-like reaction. They can down-regulate the activity of glutathione peroxidase 4 (GPX4), leading to the accumulation of lipid peroxides (LPO) and inducing ferroptosis in tumor cells. GSH depletion enhances both ferroptosis and apoptosis efficacy. Additionally, under photothermal therapy (PTT), accelerated GSH consumption and the Fenton-like reaction further amplify ferroptosis and apoptosis. Injectable hyaluronic acid-based hydrogel adheres to tumor tissue, enabling local treatment and precise PTT, thereby improving treatment efficiency. In summary, the HA-TA/MXene@Cu hydrogel synergistically enhance oxidative stress and consume GSH, triggering ferroptosis and amplifying photothermal-mediated apoptosis, leading to potent inhibition of breast cancer growth. This innovative therapeutic modality presents a promising approach for precise and effective local breast cancer treatment.</div></div>","PeriodicalId":279,"journal":{"name":"Colloids and Surfaces B: Biointerfaces","volume":"257 ","pages":"Article 115100"},"PeriodicalIF":5.6,"publicationDate":"2025-09-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145020184","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Valproic acid-based self-assembling nanoparticle prodrugs prevent skeletal muscle loss in cancer cachexia","authors":"Shoki Kamemaru ,&nbsp;Yutaka Ikeda ,&nbsp;Yukio Nagasaki","doi":"10.1016/j.colsurfb.2025.115120","DOIUrl":"10.1016/j.colsurfb.2025.115120","url":null,"abstract":"<div><div>Cancer cachexia is a multifactorial syndrome characterized by persistent skeletal muscle loss, affecting 80 % of patients with advanced cancer and accounting for 20 % of cancer-related deaths. Despite its prevalence, effective treatment options remain limited due to the side effects and poor pharmacokinetic (PK) profiles of existing therapeutics, including valproic acid (VPA). To overcome these limitations, we developed self-assembling VPA-based nanoparticle prodrugs (abbreviated as Nano^VPA), consisting of amphiphilic block copolymers, in which VPA is covalently conjugated via ester linkages. Nano^VPA significantly improved the PK profile of VPA by suppressing initial rapid plasma concentration spikes and achieving sustained VPA release, maintaining circulation for up to 48 h and enhancing skeletal muscle accumulation within 10 h post-administration. In vivo, twice-weekly administration of Nano^VPA significantly attenuated skeletal muscle loss in a cancer cachexia model by downregulating the expression of atrogin-1, a key muscle-specific ubiquitin ligase involved in proteolysis. These findings highlight the potential of Nano^VPA as a novel therapeutic strategy for cancer cachexia, offering improved efficacy and reduced dosing frequency with minimized side effects.</div></div>","PeriodicalId":279,"journal":{"name":"Colloids and Surfaces B: Biointerfaces","volume":"257 ","pages":"Article 115120"},"PeriodicalIF":5.6,"publicationDate":"2025-09-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145020185","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Magnetically controlled drug-loaded coaxial electrospun suspension fibers for promoting Schwann cell myelination","authors":"Shaolan Sun ,&nbsp;Yuqing Shang ,&nbsp;Lele Wang ,&nbsp;Mingzhu Jia ,&nbsp;Huoyun Shen ,&nbsp;Ying Han ,&nbsp;Hongxia Gao ,&nbsp;Yaqiong Liu ,&nbsp;Wenchao Guan ,&nbsp;Nana Jin ,&nbsp;Guicai Li","doi":"10.1016/j.colsurfb.2025.115098","DOIUrl":"10.1016/j.colsurfb.2025.115098","url":null,"abstract":"<div><div>Myelination of Schwann cells is essential for peripheral nerve regeneration, yet existing <em>in vitro</em> models struggle to accurately recapitulate the <em>in vivo</em> myelination process. In this study, a novel three-dimensional (3D) suspended fiber scaffold composed of a polycaprolactone (PCL) shell and an ovalbumin (OVA) core was fabricated via coaxial electrospinning to promote Schwann cell myelination. The core layer encapsulated magnetic nanoparticles loaded with curcumin and cholesterol, enabling remote, non-invasive, and magnetically controlled drug release to mimic the dynamic microenvironment during nerve repair. Among the fiber diameters tested, 10–11 μm proved most favorable for Schwann cell wrapping and myelin formation. Schwann cells adhered, migrated along, and successfully ensheathed the suspended fibers, forming myelin structures <em>in vitro</em>. Further transcriptomic analysis and protein–protein interaction (PPI) network analysis revealed synergistic upregulation of key myelination-related genes (e.g., myelin basic protein (MBP), myelin protein zero (MPZ), etc.) under the magnetically controlled drug release condition. These findings demonstrate that the designed 3D magneto-responsive coaxial suspended fibers not only provide a supportive physical scaffold but also offer a controllable biochemical environment for Schwann cell function. This work presents an innovative integration of magnetically controlled drug delivery and biomimetic scaffold design, offering a promising strategy for peripheral nerve injury repair and future nerve graft development.</div></div>","PeriodicalId":279,"journal":{"name":"Colloids and Surfaces B: Biointerfaces","volume":"257 ","pages":"Article 115098"},"PeriodicalIF":5.6,"publicationDate":"2025-09-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145027470","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Quasi-static and dynamic nanoindentation for mechano-chemical analysis on biodegradable polymeric nanocomposites for regenerative medicine","authors":"Antonio de Nigris,&nbsp;Domenico Gentile,&nbsp;Giuseppe Peter Vanoli,&nbsp;Luigi Ambrosone","doi":"10.1016/j.colsurfb.2025.115093","DOIUrl":"10.1016/j.colsurfb.2025.115093","url":null,"abstract":"<div><div>Four different biomedical patches were bioprinted using nanocomposite hydrogels of sodium alginate/gelatin, sodium alginate/gelatin/indocyanine green freely dispersed, sodium alginate/gelatin/empty liposomes and sodium alginate/gelatin/indocyanine green loaded liposomes. Quasi-static and dynamic nanoindentations of the patch surfaces were performed to examine the effect of the single component on the mechanical response. The combination of results suggests that the mechanical structure of the gels is strongly influenced by crosslinking and the liposomes incorporating dye. Indeed, the non-crosslinked samples exhibit lower <span><math><msup><mrow><mi>G</mi></mrow><mrow><mo>′</mo></mrow></msup></math></span> and <span><math><msup><mrow><mi>G</mi></mrow><mrow><mo>′</mo><mo>′</mo></mrow></msup></math></span> values, with more visco-elastic behavior. The absence of a marked increase in <span><math><msup><mrow><mi>G</mi></mrow><mrow><mo>′</mo></mrow></msup></math></span> after crosslinking, both in samples with free dye and those with dye encapsulated in liposomes, indicates that strong interactions between dye, liposomes, and ionic calcium exist. Indeed, phosphatidylcholine and indocyanine green react electrostatically with calcium cations, sequestering crosslinkers. Crosslinking with <span><math><mrow><mi>C</mi><msup><mrow><mi>a</mi></mrow><mrow><mo>+</mo><mn>2</mn></mrow></msup></mrow></math></span> improves matrix stiffness, but its effectiveness depends on the composition of the system. All results were validated with comprehensive statistical analysis among the different patches and their crosslinked counterparts.</div></div>","PeriodicalId":279,"journal":{"name":"Colloids and Surfaces B: Biointerfaces","volume":"257 ","pages":"Article 115093"},"PeriodicalIF":5.6,"publicationDate":"2025-09-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145020186","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A S/N co-doped carbon-based peroxidase-like nanozyme for the detection of AChE and organophosphorus pesticides","authors":"Zhixuan Han,&nbsp;Chenyu Zhou,&nbsp;Yuntai Lv,&nbsp;Xingguang Su","doi":"10.1016/j.colsurfb.2025.115121","DOIUrl":"10.1016/j.colsurfb.2025.115121","url":null,"abstract":"<div><div>Herein, a novel S/N co-doped carbon-based nanozyme （S/N-Fe₈₀₀） with peroxidase-like properties was synthesized by doping thiourea into Fe MOF and introducing g-C₃N₄ for pyrolysis. Generated by enzymatic cascade with acetylcholinesterase (AChE) involved, H₂O₂ could react with S/N-Fe₈₀₀ to generate reactive oxygen species (ROS). O-Phenylenediamine (OPD) could be catalyzed by ROS, resulting in the production of 2,3-diaminophenazine (DAP) with a fluorescent emission at 564 nm. The organophosphorus pesticides (OPs), including triazophos (Taz) and phosmet (Pho), have the capacity to impede the enzymic catalytic reaction of AChE, thus affect the fluorescence of DAP. A rapid and sensitive sensing platform that allows for the simultaneous determination of AChE, Taz and Pho was successfully established through monitoring the fluorescence at 564 nm. The limit of detection (LOD) for AChE, Taz and Pho were determined to be 0.035 U/L, 0.047 ng/mL and 0.072 ng/mL, respectively. This method achieved satisfactory results in the human serum and common agricultural products.</div></div>","PeriodicalId":279,"journal":{"name":"Colloids and Surfaces B: Biointerfaces","volume":"257 ","pages":"Article 115121"},"PeriodicalIF":5.6,"publicationDate":"2025-09-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145004710","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Solid Lipid Nanoparticles in Imaging, Diagnostics and Theranostics: A Review of a decade of Innovations and Clinical Applications","authors":"Pooja Tiwary ,&nbsp;Krishil Oswal ,&nbsp;Ryan Varghese ,&nbsp;Pardeep Gupta","doi":"10.1016/j.colsurfb.2025.115122","DOIUrl":"10.1016/j.colsurfb.2025.115122","url":null,"abstract":"<div><div>The clinical demand for safer, more precise, and functionally versatile imaging tools has intensified with the increasing complexity of disease diagnosis and management. Despite major strides in imaging technologies such as MRI, CT, USG, and PET/SPECT, many modalities are grappled by issues including low specificity, high systemic toxicity of contrast agents, and limited ability to provide real-time functional data. Dreaded by these shortcomings, nanotechnology-based approaches such as liposomes, quantum dots (QDs), polymeric nanoparticles (NPs), gold NPs, lipid NPs, and metallic NPs have emerged as promising alternatives. Among these, solid lipid nanoparticles (SLNs) offer a unique combination of biocompatibility, structural versatility, and surface functionalizability, positioning them as advanced carriers for next-generation imaging as well as diagnostic applications, alike. SLNs are a promising nanoplatform for the delivery of imaging and diagnostic agents owing to their biocompatibility, diversity in payload encapsulation, tunable surface properties, and potential for targeted delivery. They can enhance sensitivity and specificity across imaging modalities such as MRI, PET/SPECT, CT, and optical imaging across multiple disease states. However, despite encouraging preclinical results, SLNs still face challenges such as stability, toxicity, reproducibility, and regulatory standardization, which deter their clinical application. Future research should focus on developing multifunctional SLNs for multimodal imaging, ensuring robust safety data, and concordance with regulatory standards. The integration of diagnosis and therapy, particularly in oncology, will be pivotal. Additionally, personalizing SLNs through ligand targeting may enhance their clinical feasibility and effectiveness.</div></div>","PeriodicalId":279,"journal":{"name":"Colloids and Surfaces B: Biointerfaces","volume":"257 ","pages":"Article 115122"},"PeriodicalIF":5.6,"publicationDate":"2025-09-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145004705","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}