Colloids and Surfaces B: Biointerfaces最新文献

{"title":"Biointerfacial mechanical responses of supported lipid membranes to Gramicidin D revealed by QCM-D","authors":"Şebnem Seherler,&nbsp;Abdulhalim Kılıç","doi":"10.1016/j.colsurfb.2025.115399","DOIUrl":"10.1016/j.colsurfb.2025.115399","url":null,"abstract":"<div><div>Gramicidin D (GrD) is a well-established model ion channel–forming peptide whose mechanical interactions with supported lipid membrane architectures remain less well characterized. Here, we combine overtone-resolved Quartz Crystal Microbalance with Dissipation (QCM-D), fluorescence microscopy, and solution-phase assays to examine how GrD perturbs supported lipid bilayers (SLBs) and supported vesicular layers (SVLs) formed from neutral DOPC and anionic DOPC:DOPS (92:08). On neutral SLBs at low peptide levels, GrD induces mild outer-leaflet softening consistent with β^6.3 channel formation, producing subtle acoustic signatures that evolve with slow kinetics. In contrast, neutral SVLs exhibit immediate hydration and thickening of the vesicle shell, reflected by large dissipation increases and overtone-dependent frequency shifts. Increasing peptide levels drive both platforms into a mechanically disruptive regime characterized by distinct overtone divergence and the formation of highly dissipative peptide–lipid assemblies, including rapid disruptive remodeling on SLBs and collapse of peptide-rich outer shells on SVLs. Membrane charge further modulates these responses. Anionic SLBs and SVLs confine GrD to shallow, leaflet-restricted perturbations near the headgroup region, yielding smaller overall mass changes but strong, overtone-specific viscoelastic heterogeneity. Solution-phase turbidity and proton permeability assay are consistent with functional channel activity dominating at lower peptide levels, whereas higher peptide levels align with non-conducting, mechanically disruptive interaction states. Together, these results show that GrD exhibits distinct, architecture-dependent mechanical interaction modes governed by peptide density, membrane geometry, and surface charge, and demonstrate how QCM-D measurements on complementary SLB and SVL platforms resolve nanoscale hydration and mechanical response modes that remain inaccessible to conventional ensemble assays.</div></div>","PeriodicalId":279,"journal":{"name":"Colloids and Surfaces B: Biointerfaces","volume":"260 ","pages":"Article 115399"},"PeriodicalIF":5.6,"publicationDate":"2026-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145881526","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The cinnamaldehyde-thiosemicarbazone-zinc (II) complex induces apoptosis in CAL-27 cells","authors":"Chen-yan Li ,&nbsp;Shuohua Xie ,&nbsp;Min Deng ,&nbsp;Ganrong Huang ,&nbsp;Yanqiang Huang ,&nbsp;Shufang Li","doi":"10.1016/j.colsurfb.2025.115374","DOIUrl":"10.1016/j.colsurfb.2025.115374","url":null,"abstract":"<div><h3>Objectives</h3><div>Oral squamous cell carcinoma (OSCC) is a major malignancy affecting the oral, jaw, and facial regions. In this study, we synthesized a cinnamaldehyde-thiosemicarbazone-zinc (II) complex (CTZn) to inhibit OSCC cell proliferation.</div></div><div><h3>Methods</h3><div>We investigated the inhibitory effects of CTZn on OSCC using the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay, cell colony formation assays, wound-healing/scratch assays and transwell invasion assays. Flow cytometry (FCM) was performed to explore the CTZn's impact on apoptosis and cell-cycle progression. Through <em>in vivo</em> experiments, its antitumor activity activity was evaluated in OSCC xenograft mouse models and orthotopic tumor model mice. The mechanisms of CTZn were explored by detecting targeted metabolites, and performing drug affinity responsive target stability (DARTS) experiments, molecular docking analyses and Western blot assays. Levels of reactive oxygen species (ROS), oxidized and reduced nicotinamide adenine dinucleotide phosphate (NADP⁺/NADPH), glutathione (GSH), and 6-phosphogluconate dehydrogenase (PGD) protein in CAL-27 cells were also determined.</div></div><div><h3>Results</h3><div>These findings demonstrated that CTZn inhibited CAL-27 cell growth <em>in vitro</em> in a time- and concentration-dependent manner, with an IC₅₀ value of 1.642–2.223μmol/L, and CTZn also exerted anti-tumor activity <em>in vivo</em>. CTZn also inhibited PGD messenger RNA (mRNA), and protein expression, reduced NADP+ /NADPH and GSH levels, and significantly increased ROS levels, thereby inducing oxidative stress.</div></div><div><h3>Conclusion</h3><div>CTZn impairs mitochondrial function, decreases ATP, levels, and induces G₂-phase arrest and apoptosis in CAL-27 cells. Therefore, it is an ideal drug for treating OSCC.</div></div>","PeriodicalId":279,"journal":{"name":"Colloids and Surfaces B: Biointerfaces","volume":"260 ","pages":"Article 115374"},"PeriodicalIF":5.6,"publicationDate":"2026-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145799403","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A novel macrophage membrane-camouflaged ultra-small copper sulfide and photothermal-responsive nitric oxide donor nanocomposites for enhanced synergistic antitumor therapy","authors":"Haoyang Gong ,&nbsp;Xiaonan Li ,&nbsp;Xinru Feng ,&nbsp;Dongjing Wang ,&nbsp;Xinyi Zhang ,&nbsp;Kai Wang ,&nbsp;Yanchen Liu ,&nbsp;Lu Xu ,&nbsp;Xueyan Zhou ,&nbsp;Yanzhuo Zhang","doi":"10.1016/j.colsurfb.2025.115373","DOIUrl":"10.1016/j.colsurfb.2025.115373","url":null,"abstract":"<div><div>Ultra-small copper sulfide (CuS) nanoparticles (NPs) possess exceptional photothermal performance, Fenton-like reaction activities, and multimodal imaging characteristics, while sodium nitroprusside serves as a clinical nitric oxide (NO) donor. A judicious combination of these two agents may facilitate the development of a novel multimodal synergistic antitumor strategy. In this context, we developed macrophage membrane-camouflaged and folic acid-conjugated nanocomposites (MF-SPC) that demonstrate dual responsiveness to near-infrared (NIR) laser and pH, aiming to enhance multimodal synergistic antitumor therapy. Within these biomimetic nanocomposites, sodium nitroprusside-doped Prussian blue NPs serve as the cores for inducing photothermal-responsive NO donation, while polydopamine layer embedded with CuS dots (sub-5 nm) act as the shells. This configuration not only enhances photothermal effects but also promotes Fenton-like reaction activity, glutathione depletion capabilities, and peroxidase activity. Furthermore, the dopamine groups on the shell surface facilitate folic acid modification, drug loading, and macrophage membrane camouflage. These functionalities collectively improve tumor-targeting abilities, promote immune evasion, and enhance tumor uptake. The doxorubicin-loaded MF-SPC (MDF-SPC) exhibited favorable dispersibility, stability, and pH-responsive sustained release properties. Both <em>in vivo</em> fluorescence imaging and NIR thermal imaging show that the MDF-SPC possessed active tumor-targeting capability. Thus, the MDF-SPC demonstrated high antitumor activity and biosafety when subjected to NIR laser irradiation in 4T1 tumor-bearing mice. Overall, the active tumor-targeting and retention capabilities, along with the rapid temperature elevations produced by PTT, allow the MDF-SPC to precisely and swiftly amplify chemodynamic therapy, gas therapy, and chemotherapy, providing a novel multimodal nanoplatform to promote antitumor therapy.</div></div>","PeriodicalId":279,"journal":{"name":"Colloids and Surfaces B: Biointerfaces","volume":"260 ","pages":"Article 115373"},"PeriodicalIF":5.6,"publicationDate":"2026-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145799404","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A user-friendly multifunctional hydrogel spray with adjustable mechanical properties for hemostasis and infected wound healing","authors":"Mengyao Gao ,&nbsp;Zongliang Wang ,&nbsp;Zhaohui Tang ,&nbsp;Peibiao Zhang","doi":"10.1016/j.colsurfb.2025.115378","DOIUrl":"10.1016/j.colsurfb.2025.115378","url":null,"abstract":"<div><div>Traumatic massive hemorrhage is a critical problem in the global emergency medicine field. Traditional hemostatic materials such as gauze and sponge have problems such as poor adhesion to irregular wounds and low adhesion strength. Existing hemostatic hydrogels also face bottlenecks such as insufficient active hemostatic efficiency, challenges in balancing interface adhesion and flexibility, and a lack of functional synergy, making it difficult to meet clinical needs. To address these challenges, this study develops a self-healing hydrogel spray that can form in situ for efficient hemostasis by simply mixing oxidized dextran (ODex) with polyethyleneimine (PEI) through a dynamic Schiff base reaction. Benefiting from the 'aldehyde-amino' active crosslinking mechanism, this hydrogel spray exhibits rapid gelation, triggering crosslinking within 15 s, with an adhesion strength exceeding 10 kPa, while also possessing good self-healing properties to adapt to dynamic and irregular wound. The electrostatic physical antibacterial properties of polyethyleneimine effectively suppress both Gram-positive and Gram-negative bacteria, avoiding the risk of drug resistance. By adjusting the oxidation degree of dextran, the hemostatic-adhesion synergy of the material has been further optimized, and the hydrogel preparation process is simple, cost-effective, user-friendly and can be stored at room temperature, with good biocompatibility. Animal experiments indicate that this hydrogel spray can quickly cover and adhere to the wound, effectively stopping bleeding, while reducing the risk of infection and promoting wound healing, providing innovative strategies and technical support for the development of a new generation of clinically available traumatic hemostatic materials.</div></div>","PeriodicalId":279,"journal":{"name":"Colloids and Surfaces B: Biointerfaces","volume":"260 ","pages":"Article 115378"},"PeriodicalIF":5.6,"publicationDate":"2026-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145825382","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Hydrogel composite nanomaterials for diabetic wound treatment","authors":"Nuonan Li ,&nbsp;Xianjun Luo ,&nbsp;Hongwen Zhu ,&nbsp;Guowang Shen ,&nbsp;Meifang Li ,&nbsp;Chunxia Zhou ,&nbsp;Ning Liang ,&nbsp;Puhua Zhang ,&nbsp;Xiaoyong Zhu","doi":"10.1016/j.colsurfb.2025.115392","DOIUrl":"10.1016/j.colsurfb.2025.115392","url":null,"abstract":"<div><div>Diabetes mellitus is slowly becoming more common over the past few years. One of the most disabling effects of diabetes is chronic skin ulcers. This is because certain materials have unique physicochemical properties that make them very useful for treating diabetic wounds. The three-dimensional network structure of hydrogels allows them to absorb large amounts of water. Their ability to bind water better, hold drugs effectively, and release drugs continuously is superior. Nanomaterials, particularly helpful in preventing bacterial infections and diabetic wounds caused by oxidative stress, are defined by at least one dimension measuring under 100 nanometers. Their unique qualities include antimicrobial action, facilitation of cell migration and proliferation, drug delivery systems, antioxidant and anti-inflammatory properties. This review delineates the properties and classifications of hydrogels, emphasizing their benefits as wound dressings. It then discusses the physicochemical properties of nanomaterials and their applications in wound healing. The main focus is on the basics of hydrogel-nanomaterial composites, their preparation techniques, and the synergistic benefits of their combination as wound dressings. The utilization of hydrogel nanocomposites in diabetic wound treatment is elucidated, and prospective research avenues are suggested to offer novel insights for clinical management.</div></div>","PeriodicalId":279,"journal":{"name":"Colloids and Surfaces B: Biointerfaces","volume":"260 ","pages":"Article 115392"},"PeriodicalIF":5.6,"publicationDate":"2026-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145831890","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Pullulan-based polymeric nanosystem for KEAP1 siRNA delivery: Mitigating ROS and promoting wound healing in vitro","authors":"Gülşen Bayrak,&nbsp;Dilara Karaguzel,&nbsp;Cagatay Karaaslan,&nbsp;Işık Perçin","doi":"10.1016/j.colsurfb.2025.115352","DOIUrl":"10.1016/j.colsurfb.2025.115352","url":null,"abstract":"<div><div>Recently, siRNA delivery has shown considerable potential for therapeutic use. While commercial options facilitate efficient delivery, they can be toxic to cells to a certain degree. Therefore, using relatively less toxic and safe delivery vehicles can increase the efficiency of therapy. This study examines the utilization of Pullulan–poly(ethyleneimine) nanoparticles (Pullulan-PEI-NPs) as an effective non-viral delivery system for Kelch-like ECH-associated protein 1 (KEAP1) siRNA, with the aims of mitigating oxidative stress and facilitating wound healing in HaCaT cells by enhancing cellular antioxidant mechanisms. The Pullulan-PEI-NPs were synthesized and characterized with Dynamic Light Scattering, Nanoparticle Tracking Analysis, Fourier Transform Infrared Spectroscopy, and electron microscopy techniques. The polyplexes were formed via electrostatic interaction between Pullulan-PEI-NPs and KEAP1 siRNA, and successful siRNA delivery was confirmed by cellular uptake analysis, mRNA expression studies, and KEAP1 and nuclear factor E2-related factor 2 (NRF2) protein level assessments. Pullulan-PEI-NPs had a silencing efficiency of 72 %, which is very close to the commercial transfection agent Lipofectamine 3000 (80 %). In following studies, KEAP1 siRNA delivery with Pullulan-PEI-NPs mitigates TBHP and H₂O₂ mediated ROS and facilitates wound healing with 98.9 % wound closure in a scratch assay on HaCaT cells. Obtained results highlight the therapeutic relevance of KEAP1 silencing in redox-regulated wound regeneration. This is the first report showing that Pullulan-PEI based cationic polymeric nanoparticles can be used to deliver KEAP1 siRNA and evaluate its role in mitigating ROS and increasing wound healing. These results highlight the promise of Pullulan-PEI-NPs as a safe and adaptable platform for non-viral gene therapy applications.</div></div>","PeriodicalId":279,"journal":{"name":"Colloids and Surfaces B: Biointerfaces","volume":"260 ","pages":"Article 115352"},"PeriodicalIF":5.6,"publicationDate":"2026-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145792840","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Advances in coagulation peptides: Exploring diverse functions from hemostasis to disease treatment","authors":"Wenwen Ma ,&nbsp;Yumei Wang ,&nbsp;Xin Liu ,&nbsp;Wen Li ,&nbsp;Wenxue Zhao ,&nbsp;Jumeng Di ,&nbsp;Hailin Cong ,&nbsp;Bing Yu","doi":"10.1016/j.colsurfb.2025.115383","DOIUrl":"10.1016/j.colsurfb.2025.115383","url":null,"abstract":"<div><div>Coagulation dysfunction and bleeding control remain major challenges in trauma, surgery, and interventional therapy. Millions of patients die from excessive blood loss every year. Traditional hemostatic materials such as gelatin sponge, chitosan, and zeolite have limitations such as a single mechanism of action and poor biological stability. At the same time, clotting peptides have attracted much attention due to their low molecular weight, high biological activity, and precisely designed molecular structure. This paper systematically reviews the latest progress of coagulation peptides as a new hemostatic material, discusses their interaction mechanism with coagulation factors and platelet receptors, and analyzes the structural design strategy. Subsequently, this article critically analyzed the clinical trial data, efficacy comparison, and long-term safety evaluation of clotting peptides in the fields of gastrointestinal endoscopy, high-risk surgery, and systemic hemostasis. Finally, this article summarizes the key clinical translational barriers faced by the study of clotting peptides, including challenges such as long-term biocompatibility, potential thrombosis risks, and in vivo degradation characteristics. This review aims to provide systematic theoretical guidance and objective clinical transformation analysis for the rational design and performance optimization of a new generation of multifunctional coagulation peptide materials.</div></div>","PeriodicalId":279,"journal":{"name":"Colloids and Surfaces B: Biointerfaces","volume":"260 ","pages":"Article 115383"},"PeriodicalIF":5.6,"publicationDate":"2026-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145838041","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Microrobots/micromotors in cancer therapeutics, advances and applications","authors":"Hezhe Huang,&nbsp;Yuqing Miao,&nbsp;Yuhao Li","doi":"10.1016/j.colsurfb.2025.115394","DOIUrl":"10.1016/j.colsurfb.2025.115394","url":null,"abstract":"<div><div>Cancer remains one of the most challenging diseases to conquer due to its high mortality rate and the lack of effective diagnostic and therapeutic tools. Microrobot technology has brought revolutionary advancements to the field of tumor medicine. By leveraging its micro/nanoscale dimensions and precise propulsion and navigation capabilities, it has enhanced the accuracy and efficiency of cancer diagnosis and treatment. This review provides a detailed exploration of microrobots’ motion from the perspectives of propulsion and navigation. It systematically summarizes its key applications in cancer therapy. Microrobots, which utilize gas, magnetic, light, or ultrasound-based propulsion mechanisms, and are combined with asymmetrical morphologies or biotargeted navigation, can traverse complex biological barriers such as the blood-brain barrier and mucus barrier. They enable precise delivery and controlled release of anticancer drugs. In the realm of diagnosis and imaging, microrobots, by carrying fluorescent probes or contrast agents, have improved the sensitivity of tumor detection and the resolution of deep tissue imaging. Furthermore, microrobots, when coordinated with photothermal therapy, sonodynamic therapy, or immunotherapy, have enhanced tumor eradication effects. They also activate immune responses to inhibit metastatic lesions. Finally, this review provides an outlook on the prospects of microrobots in tumor medicine, proposing potential collaborative pathways with emerging technologies. It highlights their vast potential in improving therapeutic efficacy and reducing side effects.</div></div>","PeriodicalId":279,"journal":{"name":"Colloids and Surfaces B: Biointerfaces","volume":"260 ","pages":"Article 115394"},"PeriodicalIF":5.6,"publicationDate":"2026-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145861605","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Frankincense-loaded lactoferrin-conjugated solid lipid nanoparticles for targeted brain delivery and neuroprotection in a scopolamine-induced Alzheimer’s model","authors":"Farimah Moazzam ,&nbsp;Ashrafalsadat Hatamian-Zarmi ,&nbsp;Marzie Sabaghian ,&nbsp;Khadijeh Khezri ,&nbsp;Bahman Ebrahimi Hosseinzadeh ,&nbsp;Fariba Khodagholi ,&nbsp;Moloud Jalili Shahmansouri ,&nbsp;Fatemeh Sadat Rashidi","doi":"10.1016/j.colsurfb.2025.115370","DOIUrl":"10.1016/j.colsurfb.2025.115370","url":null,"abstract":"<div><div>Central nervous system (CNS) disorders remain a major field with substantial unmet therapeutic needs. This study aimed to address the critical challenge of brain drug delivery by employing solid lipid nanoparticles (SLNs) surface-functionalized with lactoferrin (Lf) ligands, from the transferrin family, to facilitate transport across the blood–brain barrier. Frankincense (F), a natural compound with well-documented neuroprotective properties and minimal adverse effects, was encapsulated within (SLNs) using a microemulsion approach. The optimal formulation resulted in nanoparticles (NPs) with an average size of 103.1 ± 0.9 nm, an encapsulation efficiency of 95.2 ± 0.8 %, a drug loading capacity of 8.6 ± 0.1 %, a polydispersity index (PDI) of 0.3 ± 0.08, and a zeta potential of −29.2 ± 0.5 mV. The optimized NPs showed a sustained drug release profile, and ATR-FTIR spectra confirmed the conjugation of lactoferrin to the nanoparticles. To establish translational relevance, the neuroprotective effectiveness of (F-Lf-SLNs) was investigated using a scopolamine-induced Alzheimer's disease animal model. Behavioral tests, along with biochemical and histological analyses, were conducted. The findings demonstrated that (F-Lf-SLNs) significantly improved the brain delivery of frankincense, highlighting their promise as a neuroprotective strategy for CNS disorders. They also effectively protected neurons compared to the scopolamine-induced group. Overall, these results emphasize the key role of drug delivery systems and ligand-targeting methods in enhancing the therapeutic effectiveness of drugs.</div></div>","PeriodicalId":279,"journal":{"name":"Colloids and Surfaces B: Biointerfaces","volume":"260 ","pages":"Article 115370"},"PeriodicalIF":5.6,"publicationDate":"2026-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145754008","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The MCM/Lys-Cys nanodevices for the efficient gene delivery: An approach towards MCP1 gene manipulation using CRISPR technology","authors":"Azadeh Rahimi ,&nbsp;Ilnaz Rahimmanesh ,&nbsp;Navid Abedpoor ,&nbsp;Maryam Boshtam ,&nbsp;Elham Bidram ,&nbsp;Shaghayegh Haghjooy Javanmard ,&nbsp;Hossein Khanahmad ,&nbsp;Laleh Rafiee ,&nbsp;Ashkan Bigham ,&nbsp;Mohammad Rafienia ,&nbsp;Saeed Karbasi ,&nbsp;Laleh Shariati","doi":"10.1016/j.colsurfb.2025.115377","DOIUrl":"10.1016/j.colsurfb.2025.115377","url":null,"abstract":"<div><div>Breast cancer continues to be the most common malignancy among women worldwide, requiring novel therapeutic approaches. This research investigates an innovative gene delivery strategy employing mesoporous silica nanoparticles (MCM-41) modified with lysine and cysteine (Lys-Cys) for the effective delivery of CRISPR-Cas9 plasmids aimed at the monocyte chemoattractant protein-1 (MCP-1/CCL2) gene. Bioinformatics analysis of the TCGA-BRCA dataset revealed substantial deregulation of CCL2 in breast cancer, underscoring its involvement in tumor growth and inflammation. The MCM/Lys-Cys nanocarrier demonstrated remarkable biocompatibility and effectively encapsulated a plasmid containing GFP, promoting superior cellular uptake in MDA-MB-231 breast cancer cells compared to conventional techniques. Functional experiments demonstrated that CRISPR/Cas9-mediated suppression of CCL2 markedly decreased cell proliferation, migration, and invasion, highlighting the promise of this targeted gene therapy strategy in breast cancer management. The findings indicate that the MCM/Lys-Cys nanosystem presents a viable non-viral approach for precise gene editing, potentially boosting therapeutic efforts against breast cancer by modulating inflammatory pathways.</div></div>","PeriodicalId":279,"journal":{"name":"Colloids and Surfaces B: Biointerfaces","volume":"260 ","pages":"Article 115377"},"PeriodicalIF":5.6,"publicationDate":"2026-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145825404","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}