Colloids and Surfaces B: Biointerfaces最新文献

{"title":"Paving a pathway for bright red-emitting Eu-loaded albumin nanoparticles fabrication","authors":"Pavel Khramtsov ,&nbsp;Zarina Galaeva ,&nbsp;Ekaterina Khramtsova ,&nbsp;Rustem Zairov ,&nbsp;Alexey Dovzhenko ,&nbsp;Vadim Vasilyev ,&nbsp;Artem Minin ,&nbsp;Maria Bochkova ,&nbsp;Mikhail Rayev","doi":"10.1016/j.colsurfb.2025.115140","DOIUrl":"10.1016/j.colsurfb.2025.115140","url":null,"abstract":"<div><div>Lanthanide complexes with organic ligands exhibit unique photophysical properties, including long-lived emission lifetimes, large Stokes shifts, and sharp emission bands, making them highly attractive for bioanalytical and biomedical applications. Here, we report the first successful use of the desolvation method to synthesize bovine serum albumin (BSA) nanoparticles loaded with europium complexes. This simple approach involved the dropwise addition of an ethanolic solution of europium complex precursors into an aqueous BSA solution. We systematically optimized synthesis parameters to produce nanoparticles with high brightness and low polydispersity. Under optimized conditions, the resulting nanoparticles exhibited a uniform size of 150–160 nm (PDI&lt;0.15) as determined by dynamic light scattering and electron microscopy. Each nanoparticle encapsulated approximately 350 europium complexes, achieving quantum yields of 0.77–1.18 % with excitation/emission maxima at 360/615 nm. The synthesis yield was ∼80 %, and the nanoparticles demonstrated excellent size stability across physiological pH conditions and after three months of storage. However, luminescence intensity decreased over time and under certain buffer conditions. Cytotoxicity studies using Vero cells showed cell viability above 80 % at nanoparticle concentrations up to 1 mg/mL. The key challenges to be addressed are the improvement of synthesis reproducibility and the stability of luminescent properties.</div></div>","PeriodicalId":279,"journal":{"name":"Colloids and Surfaces B: Biointerfaces","volume":"257 ","pages":"Article 115140"},"PeriodicalIF":5.6,"publicationDate":"2025-09-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145074302","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Methyl gallate-loaded platelet-mimetic liposome for targeted therapy of ulcerative colitis","authors":"Lu Wang ,&nbsp;Qingze Fan ,&nbsp;Zhigang Chen ,&nbsp;Tianci Li ,&nbsp;Xiaoxuan Li ,&nbsp;Qiaozhi Wang ,&nbsp;Ruizhi Tan ,&nbsp;Lishang Liao ,&nbsp;Wenjun Zou ,&nbsp;Jianming Wu ,&nbsp;Shengli Wan","doi":"10.1016/j.colsurfb.2025.115141","DOIUrl":"10.1016/j.colsurfb.2025.115141","url":null,"abstract":"<div><div>Ulcerative colitis (UC) is a persistent inflammatory bowel disease that poses an increasingly significant public health concern worldwide. Given the limited efficacy of current therapies, the development of novel treatment approaches has become increasingly urgent. Our previous investigation established that methyl gallate (MG) exhibits therapeutic potential for UC due to its effective anti-inflammatory properties. However, the low targeting specificity and high <em>in vivo</em> clearance rate of MG restrict its application. Herein, platelet membrane (PM) biomimetic MG-loaded liposome (PML) was firstly constructed to improve MG delivery and enable targeted UC therapy. Due to the PM cloaking, PML exhibited prolonged circulation time and enhanced targeting to damaged colon caused by UC. Moreover, the PM biomimetic liposome demonstrated higher uptake efficiency in inflammatory cells. <em>In vitro</em> and <em>in vivo</em> investigations consistently proved that PML exhibited significant anti-inflammatory activity. Additionally, intravenous administration of PML effectively reduced disease severity in UC mouse models and promoted the intestinal tissue repair. Besides, PML showed high erythrocyte compatibility and favorable biosafety in UC mice and zebrafish. Collectively, PML achieve enhanced MG delivery and may provide a novel precision therapy strategy for UC treatment.</div></div>","PeriodicalId":279,"journal":{"name":"Colloids and Surfaces B: Biointerfaces","volume":"257 ","pages":"Article 115141"},"PeriodicalIF":5.6,"publicationDate":"2025-09-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145084725","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"3D molybdenum disulfide doped sodium alginate wound dressing with rapid photothermal response","authors":"Yiqing Deng ,&nbsp;Yihao Luo ,&nbsp;Xingyun Pu ,&nbsp;Xu Peng ,&nbsp;Can Cheng ,&nbsp;Shaoxiong Feng ,&nbsp;Xixun Yu","doi":"10.1016/j.colsurfb.2025.115139","DOIUrl":"10.1016/j.colsurfb.2025.115139","url":null,"abstract":"<div><div>A novel multifunctional hydrogel wound dressing, Gel/MoS₂, was developed by integrating 3D spherical molybdenum disulfide (MoS₂) nanospheres into a robust dual-network polymer matrix. This matrix was constructed from dopamine-modified sodium alginate (SA-DA) and polyvinyl alcohol (PVA), offering enhanced tissue adhesion and mechanical strength. The straightforward hydrothermal synthesis of our unique 3D MoS₂ nanospheres yielded a material with superior photothermal performance compared to commercial 2D MoS₂ nanosheets. Upon irradiation with near-infrared light (808 nm), the prepared hydrogel—specifically Gel/M₁₀, which refers to the composite hydrogel with a MoS₂ doping concentration of 10 mg—exhibited a potent photothermal effect. It rapidly reached a therapeutic temperature of 57.4 °C within 5 min, demonstrating an in vitro antibacterial efficacy exceeding 90 %. Furthermore, the Gel/M₁₀ hydrogel exhibited excellent peroxidase-like activity, effectively eliminating diverse ROS and reducing oxidative stress. In vivo experiments confirmed that under NIR irradiation, Gel/M₁₀ significantly accelerated wound closure and promoted tissue regeneration. This work introduces a highly efficient integrated hydrogel system, demonstrating the spherical MoS₂'s capability to serve as an advanced photothermal agent for antibacterial wound dressings.</div></div>","PeriodicalId":279,"journal":{"name":"Colloids and Surfaces B: Biointerfaces","volume":"257 ","pages":"Article 115139"},"PeriodicalIF":5.6,"publicationDate":"2025-09-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145046278","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Surface engineering of yeast microcarriers to enhance droplet retention and antimicrobial efficacy on hydrophobic plant surfaces","authors":"Meihan Tao ,&nbsp;Ayoub Abdollahi ,&nbsp;Geoff R. Willmott ,&nbsp;Siew-Young Quek ,&nbsp;Kang Huang","doi":"10.1016/j.colsurfb.2025.115132","DOIUrl":"10.1016/j.colsurfb.2025.115132","url":null,"abstract":"<div><div>Microbial contamination on plant surfaces is a major concern in both preharvest and postharvest settings. Although spraying is widely used for applying pesticides and sanitizers, poor droplet retention on hydrophobic surfaces results in significant chemical loss, environmental pollution, and health risks. Yeast-based microcarriers have shown strong potential for delivering a broad spectrum of antimicrobial agents, yet their droplet impact behavior remains poorly understood. Herein, we systematically analyzed the droplet dynamics of yeast suspensions using two retention-enhancing strategies: (1) spray modification with food-grade additives, including sodium dodecyl sulfate (SDS) and glycerol, and (2) surface engineering with ε-poly-L-lysine (ε-pLL) and SDS to form micelle-like coatings. High-speed imaging was used to visualize droplet dynamics on transparent PDMS replicas of basil leaves, enabling controlled analysis of fluid behavior on microstructured, hydrophobic surfaces. Engineered yeast droplets exhibited strong adhesion and formed large, symmetric residuals upon impact. While SDS and glycerol modifications led to modest improvements in retention, surface-engineered yeast consistently outperformed them, demonstrating the effectiveness of layer-by-layer coatings in enhancing droplet deposition on hydrophobic plant surfaces. To demonstrate delivery potential, <em>in-situ</em> synthesized silver–copper bimetallic nanoparticles (AgCu biNPs) were encapsulated within yeast carriers. When applied to <em>E. coli</em>-inoculated basil leaves inclined at 30 °, biNPs delivered via engineered yeast achieved a 2.8-log CFU/cm² reduction, compared to just 0.73-log for native yeast carriers. These findings demonstrate that surface-engineered yeast microcarriers enhance both droplet retention and antimicrobial efficacy, offering a promising, bio-based strategy to reduce spray loss and improve delivery performance in agricultural and food safety applications.</div></div>","PeriodicalId":279,"journal":{"name":"Colloids and Surfaces B: Biointerfaces","volume":"257 ","pages":"Article 115132"},"PeriodicalIF":5.6,"publicationDate":"2025-09-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145044836","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Bio-based pectin films with cannabidiol extract from pollen of Cannabis sativa L - active packaging to protect anthocyanins in Thomson seedless dark grapes","authors":"Renata Dobrucka ,&nbsp;Marcin Szymański","doi":"10.1016/j.colsurfb.2025.115133","DOIUrl":"10.1016/j.colsurfb.2025.115133","url":null,"abstract":"<div><div>In the present study, polysaccharide films based on pectin with an extract from pollen of <em>Cannabis sativa L</em>. were developed. <em>Thompson seedless</em> grapes were packed in the resulting films to assess the stability of anthocyanins in stored fruit. Fruit that were packed in 0.5 F, 1.0 F films were characterised by a gradual increase in anthocyanins after 8,11 and 14 days. For fruit stored in 2.0 F film, the increase in anthocyanins was small (p &gt; 0.05). After 14 days of storage, the value of anthocyanins in the fruit was 0.51 ± 0.02 [%]. In combination with the obtained slow changes in L*, a*, b* parameters for the packaged fruit, it can be concluded that the film limited access to light and oxygen, which slowed down the degradation and oxidation reactions of anthocyanins, as well as the excessive synthesis of these pigments. The resulting eco-friendly packaging film with extract from pollen of <em>Cannabis sativa L.</em>, in addition to meeting the requirements of a circular economy, can effectively protect against loss of quality and shelf life of the fruit.</div></div>","PeriodicalId":279,"journal":{"name":"Colloids and Surfaces B: Biointerfaces","volume":"257 ","pages":"Article 115133"},"PeriodicalIF":5.6,"publicationDate":"2025-09-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145044837","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A NIR-responsive upconversion implant for wireless photodynamic therapy of tumors","authors":"Cijun Shuai ,&nbsp;Jie Zeng ,&nbsp;Zhaoxiong Zhang ,&nbsp;YouWen Yang ,&nbsp;MingLi Yang ,&nbsp;Hengyun Ye ,&nbsp;Pan He ,&nbsp;Jun Zan ,&nbsp;Jun Zhang","doi":"10.1016/j.colsurfb.2025.115129","DOIUrl":"10.1016/j.colsurfb.2025.115129","url":null,"abstract":"<div><div>Photosensitizer-mediated photodynamic therapy (PDT) enables wireless tumor inactivation by releasing cytotoxic reactive oxygen species (ROS). Nevertheless, the activation of conventional photosensitizers typically depends on visible light with limited tissue penetration, thereby restricting their therapeutic efficacy in deep-seated tumors. To overcome this limitation, an upconversion implant was engineered to function as a flexible internal light-emitting device by converting deeply penetrating near-infrared-I (NIR-I) light into visible light. Specifically, a composite PDT nanosystem was constructed by linking NaYF₄:Yb,Er upconversion nanoparticles with the rose bengal photosensitizer—whose emission and absorption spectra are well-matched—using polyvinylpyrrolidone (PVP) as a molecular bridge. This PDT nanosystem was subsequently incorporated into poly-L-lactic acid (PLLA) scaffolds fabricated via selective laser sintering. Under 980 nm laser irradiation, the upconversion implant was capable of wirelessly emitting upconverted visible light through more than 2 cm of an in vitro tissue model and generating abundant ROS via the photodynamic effect. In vitro cell experiments demonstrated that the system effectively eliminated tumor cells by damaging the cell membrane and cellular genetic material. These results suggest that the scaffolds possess significant potential as anti-tumor tissue implants and offer a promising avenue for the development of innovative tumor treatment strategies.</div></div>","PeriodicalId":279,"journal":{"name":"Colloids and Surfaces B: Biointerfaces","volume":"257 ","pages":"Article 115129"},"PeriodicalIF":5.6,"publicationDate":"2025-09-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145046277","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Antibody functionalized targeted novel epigenetic nanotherapy for paediatric neuroblastoma","authors":"Avinash Chandra Kushwaha,&nbsp;Mohd Ayoub,&nbsp;Devraj Sarkar,&nbsp;Surajit Karmakar,&nbsp;Subhasree Roy Choudhury","doi":"10.1016/j.colsurfb.2025.115137","DOIUrl":"10.1016/j.colsurfb.2025.115137","url":null,"abstract":"<div><div>The neuroblastoma is the most heterogeneous, malignant, extracranial, childhood cancer of the embryonic sympathoadrenal lineage of the neural crest which mainly affect the adrenal glands. The polycomb protein, Bmi1, is highly expressed in the neuroblastoma tumorigenesis and executes epigenetic regulation of several downstream markers through its E3 ligase activity. The suppression of Bmi1 with a specific inhibitor, PRT4165, possesses a therapeutic potential but limitations such as off targeting impede its applications. In order to manifest Bmi1-specific targeted nanotherapy, PRT4165-encapsulated and anti-GD2-decorated (for active targeting of neuroblastoma) human serum albumin nanoparticles (PRT@HSANPs@GD2) are introduced as neuroblastoma therapy. PRT@HSANPs@GD2 enhance the cellular internalization and cytotoxicity through apoptosis in the GD2⁺ neuroblastoma cells. Furthermore, PRT@HSANPs@GD2 exhibit superior regression of tumor volumes, and downregulation of Bmi1. In addition, we report Bmi1 and Oct3/4 along with Oct3/4 and Vimentin interactions in neuroblastoma for the first-time wherein our nanoformulation, PRT@HSANPs@GD2, repress the Bmi1 and Oct3/4 along with Oct3/4 and Vimentin interactions in neuroblastoma. The GD2 targeting through nanoparticles-encapsulated with PRT4165 is an innovative and novel targeted nanotherapy regimen for the neuroblastoma with a future potential of translational development.</div></div>","PeriodicalId":279,"journal":{"name":"Colloids and Surfaces B: Biointerfaces","volume":"257 ","pages":"Article 115137"},"PeriodicalIF":5.6,"publicationDate":"2025-09-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145079270","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Selective and spatiotemporal‑resolved nano-PROTAC for lung cancer therapy","authors":"Xiaowei Xu ,&nbsp;Le Wang ,&nbsp;Shitao Lin ,&nbsp;Xiaoqing Wu ,&nbsp;Xufeng Lin ,&nbsp;Xiaoling Guan ,&nbsp;Shiqi Tang ,&nbsp;Ni Zhang ,&nbsp;Yuxin Zhang ,&nbsp;Lingmin Zhang ,&nbsp;Dazhi Zhou","doi":"10.1016/j.colsurfb.2025.115135","DOIUrl":"10.1016/j.colsurfb.2025.115135","url":null,"abstract":"<div><div>The Proteolysis-Targeting Chimeras (PROTAC) technology has become a promising tool in lung cancer therapy. The PROTAC regents targeting to Bromodomain-containing Protein 4 (BRD4) showed effective induction of apoptosis in lung cancer cells. However, the application of this kind of PROTAC regents is still hindered by the low tissue specificity, solubility, and detectability. Herein, we developed a selective and spatiotemporal‑resolved nano-PROTAC, which was constructed using the functional polymer Poly(lactic-co-glycolic acid)-Polyethylene glycol 2000-Maleimide (PLGA-PEG-Mal) to load PROTAC regent dBET6 and the fluorescence dye, and further modification with aptamer targeting to the lung cancer cells. The drug-loaded nanoparticles can be uptaken by lung cancer cells effectively, inducing the complete degradation of BRD4 and effective apoptosis of lung cancer cells. In vivo experiments indicated that the aptamer-modified nanoparticles accumulated in the tumors, which led to the effective suppression of tumor growth in the tumor-bearing mouse model. Furthermore, this type of nano-PROTAC was endowed with real-time tracking capability, avoiding the extra and tedious modifications. The integrated diagnosis and treatment system showed great promise in lung cancer therapy.</div></div>","PeriodicalId":279,"journal":{"name":"Colloids and Surfaces B: Biointerfaces","volume":"257 ","pages":"Article 115135"},"PeriodicalIF":5.6,"publicationDate":"2025-09-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145061137","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Engineered polysaccharide scaffolds for cartilage regeneration: Mechanisms, functionalization, and clinical prospects","authors":"Binglun Zhang ,&nbsp;Meng Zhang ,&nbsp;Chao Jiang ,&nbsp;Wei Yan ,&nbsp;Yilong Pan ,&nbsp;Fanhe Meng","doi":"10.1016/j.colsurfb.2025.115134","DOIUrl":"10.1016/j.colsurfb.2025.115134","url":null,"abstract":"<div><div>Polysaccharides, naturally occurring long-chain carbohydrates composed of repeated monosaccharide units linked by glycosidic bonds, have emerged as promising biomaterials for cartilage tissue engineering due to their biocompatibility, biodegradability, and ability to mimic the natural extracellular matrix. This review explores the roles of polysaccharides, such as chitosan, alginate, hyaluronic acid (HA), and chondroitin sulfate, in cartilage repair, focusing on their applications in scaffold design, cell differentiation, and mechanical property enhancement. Polysaccharide-based scaffolds support chondrogenesis, regulate inflammation, and promote tissue regeneration, making them ideal candidates for cartilage repair. Functionalization with antimicrobial agents and bioactive molecules further improves their efficacy by preventing infection and promoting cell attachment and differentiation. However, challenges related to scalability, biocompatibility, stability <em>in vivo</em>, and cost-effective production remain exist. The review also discusses emerging trends, including the integration of 3D bioprinting, nanomaterials, and combination therapies to enhance polysaccharide-based scaffolds for cartilage repair. The future of polysaccharide-based scaffolds holds great promise in advancing cartilage tissue engineering toward clinical applications.</div></div>","PeriodicalId":279,"journal":{"name":"Colloids and Surfaces B: Biointerfaces","volume":"257 ","pages":"Article 115134"},"PeriodicalIF":5.6,"publicationDate":"2025-09-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145090935","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Facile synthesis of Silicalite-1 with tunable porous architectures via desilication-recrystallization: Enhanced Doxorubicin delivery and inhibited growth against colorectal cancer","authors":"Wenjing Li ,&nbsp;Juan Shao ,&nbsp;Chuxin Zhang ,&nbsp;Guodong Cao ,&nbsp;Yitao Yan ,&nbsp;Qiongyi Han ,&nbsp;Huimei Yu ,&nbsp;Yingying Wei ,&nbsp;Wenbo Zhang ,&nbsp;Jie Zheng ,&nbsp;Zhongyuan Guo","doi":"10.1016/j.colsurfb.2025.115136","DOIUrl":"10.1016/j.colsurfb.2025.115136","url":null,"abstract":"<div><div>Colorectal cancer has emerged as a leading cause of global cancer-related morbidity and mortality, necessitating innovative therapeutic strategies. We developed a desilication-recrystallization method to synthesize Silicalite-1-X (X = 2, 8, 16, 32), a hierarchical porous silica molecular sieve with cost-effectiveness, facile synthesis, and tunable porosity. Its programmable mesoporous structure is achieved by precisely controlling the desilication-recrystallization kinetics. Tunable mesopores with precise diameters of 4–6 nm and 20–30 nm were fabricated by controlling cavity expansion through temporal modulation (2–32 h) of a time-dependent synthesis process. Detailed analysis of drug loading and release profiles revealed that although pore structure modification did not significantly alter drug loading efficiency (DLE), it induced a slight but consistent improvement in drug release rate as pore size increased. Systematic biocompatibility evaluation was conducted through an <em>in vitro</em> human colonic epithelial tissue model complemented by the KM mice <em>in vivo</em> studies. Histopathological analysis confirmed Silicalite-1-X caused minimal cytotoxicity and oxidative stress in healthy colon tissue, preserved mucus secretion, and showed no systemic toxicity. Furthermore, Doxorubicin (DOX) loaded Silicalite-1-X platform (Silicalite-1-DOX-X) exhibited dose-dependent cytotoxic efficacy against colorectal carcinoma <em>in vitro</em> models, confirming its anti-tumor therapeutic potential. Our findings provide a framework for engineering molecular sieves with tunable porosity and a platform for expanding their multifunctional applications in advanced materials.</div></div>","PeriodicalId":279,"journal":{"name":"Colloids and Surfaces B: Biointerfaces","volume":"257 ","pages":"Article 115136"},"PeriodicalIF":5.6,"publicationDate":"2025-09-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145046276","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}