Colloids and Surfaces B: Biointerfaces最新文献

{"title":"PEGylated PAMAM dendrimer nanoplatform for co-delivery of chemotherapeutic agents and inorganic nanoparticles enhancing chemo-photothermal combination therapy","authors":"Binzhong Lu ,&nbsp;Yingying Yang ,&nbsp;Xiang Li ,&nbsp;Qiuli Cheng ,&nbsp;Leitao Zhang ,&nbsp;Wenlan Wu ,&nbsp;Junbo Li","doi":"10.1016/j.colsurfb.2025.114688","DOIUrl":"10.1016/j.colsurfb.2025.114688","url":null,"abstract":"<div><div>Chemo-photothermal combination therapy has emerged as an important approach for enhancing therapeutic efficacy against tumors. However, developing a flexible nanoplatform capable of co-encapsulating inorganic photothermal agents (PTAs) and organic antitumor drugs remains challenging. A polyethylene glycol-functionalized polyamidoamine (PAMAM) dendrimer (PAMAM-PEG) served as a template for the synthesis of copper sulfide (CuS) nanoparticles and subsequent encapsulation of doxorubicin (DOX) within its inner cavities. The multifunctional nanoplatform demonstrated high colloidal stability along with photothermal conversion efficiency upon 980 nm laser irradiation. This synergistic effect substantially improved DOX cellular uptake and tumor penetration, resulting in superior antitumor efficacy relative to chemotherapy alone. These results demonstrate that PAMAM-PEG represents a promising nanoplatform for combined chemo-photothermal therapy, providing a novel strategy to address current limitations in tumor treatment while enhancing therapeutic outcomes.</div></div>","PeriodicalId":279,"journal":{"name":"Colloids and Surfaces B: Biointerfaces","volume":"252 ","pages":"Article 114688"},"PeriodicalIF":5.4,"publicationDate":"2025-04-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143820925","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Treatment of ulcerative colitis by sustained release curcumin pellets coated with ethyl ascorbic acid; in-vitro, in-vivo, and in-silico analysis","authors":"Dure Shahwar ,&nbsp;Muhammad Hanif ,&nbsp;Qasim Umar ,&nbsp;Muhammad Rafiq ,&nbsp;Khalid Mahmood ,&nbsp;Muhammad Qaiser ,&nbsp;Nabeela Ameer ,&nbsp;Muhammad Haseeb llyas","doi":"10.1016/j.colsurfb.2025.114671","DOIUrl":"10.1016/j.colsurfb.2025.114671","url":null,"abstract":"<div><div>The present work aimed to protect the rapid reduction of Curcumin (Cur) in alkaline pH by newly synthesized ethyl ascorbic acid (EAA) in dual-coated Cur pellets (CCur-P4d) used for the treatment of inflammatory bowel disease (IBD). Physicochemical and morphological analysis was performed by FTIR, DSC, XRD, and SEM-EDS and in vitro release study. 92.87 ± 6.26 % release of Cur from uncoated Cur pellets (UCur-P4) in 0.1 M HCl at pH 1.2 was controlled to 5 % by coating with Eudragit L-100 (EL-100) which interns increased to 97.99 ± 7.35 % in 100 mM phosphate buffer of pH 7.4. The scavenging activity of 2,2-Diphenyl-1-picrylhydrazyl (DPPH) and hydrogen peroxides (H₂O₂) was 95.88 ± 1.22 % and 85.35 ± 1.43 %, respectively. Acute toxicity study and anti-ulcerative effect of CCur-P4d in ulcerative colitis (UC) induced albino rats were evaluated by microscopic and macroscopic analysis. In protein-ligand binding interaction, Cur showed a potent inhibitory effect on inflammatory markers C-reactive protein, IL-6, and TNF-α. Conclusively, CCur-P4d is considered an alternative to locally acting therapeutics agents used in IBD.</div></div>","PeriodicalId":279,"journal":{"name":"Colloids and Surfaces B: Biointerfaces","volume":"252 ","pages":"Article 114671"},"PeriodicalIF":5.4,"publicationDate":"2025-04-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143824431","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Enhanced cancer therapy using modified magnetic α-Fe2O3/Fe3O4 nanorods: Dual role in curcumin delivery and ferroptosis induction","authors":"Mingyi Ma ,&nbsp;Zhongjun Pan ,&nbsp;Ziye Zhu ,&nbsp;Chen Ling ,&nbsp;Jiahao Yuan ,&nbsp;Xiangdong Huo ,&nbsp;Shasha Li ,&nbsp;Ruijiang Liu","doi":"10.1016/j.colsurfb.2025.114689","DOIUrl":"10.1016/j.colsurfb.2025.114689","url":null,"abstract":"<div><div>Curcumin (CUR) has gained considerable attention in oncology due to its potent anti-tumor, anti-inflammatory, and antioxidant properties. However, its clinical utility was significantly hindered by low bioavailability and rapid metabolic degradation. This work designed a magnetic α-Fe₂O₃/Fe₃O₄ heterogeneous nanorod prepared via a urea hydrolysis-calcination process for curcumin delivery. The nanorods were modified with hyaluronic acid (HA), providing a stable matrix for curcumin encapsulation. The zeta potential of α-Fe₂O₃/Fe₃O₄/HA/CUR was −3.94 mV, the saturation magnetization was 7.82 emu·g⁻¹, the encapsulation rate and drug loading rate were 10.53 % and 29.64 % respectively. At pH 5.4, 6.5, and 7.4, the release rates were 44.8 %, 40.2 %, and 39.3 %, respectively. Kinetic modeling indicated that release profiles followed the Weibull kinetic model, with an R² value greater than 0.98. The α-Fe₂O₃/Fe₃O₄/HA/CUR exhibited excellent magnetic responsiveness, demonstrating a significant inhibitory effect on human liver cancer (HepG2) cells (inhibition rate greater than 60 %) under a magnetic field. Moreover, they substantially reduced the cytotoxicity of curcumin on normal human (LO2) cells. The α-Fe₂O₃/Fe₃O₄/HA/CUR inhibited migration and proliferation of HepG2 cells, induced apoptosis via the Caspase pathway, and synergistically suppressed tumor cell development through ferroptosis. The drug release from the α-Fe₂O₃/Fe₃O₄/HA/CUR was stable, significantly enhancing the bioavailability of curcumin. This provided a promising strategy for improving the bioavailability of poorly soluble drugs and enhancing liver cancer treatment outcomes.</div></div>","PeriodicalId":279,"journal":{"name":"Colloids and Surfaces B: Biointerfaces","volume":"252 ","pages":"Article 114689"},"PeriodicalIF":5.4,"publicationDate":"2025-04-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143824432","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Hybrid structure combining essential oil derivatives and polydopamine for anti-bacterial coatings","authors":"Nathan Jourdainne ,&nbsp;Dimitri Mercier ,&nbsp;Bruna Costa ,&nbsp;Filipa Campos ,&nbsp;Claudia Monteiro ,&nbsp;Natália Costa ,&nbsp;M. Cristina L. Martins ,&nbsp;Pascal Thébault","doi":"10.1016/j.colsurfb.2025.114679","DOIUrl":"10.1016/j.colsurfb.2025.114679","url":null,"abstract":"<div><div>The development of effective antimicrobial surfaces is crucial for reducing the risk of medical device-associated infections. This study investigates the antibacterial potential of carvacrol (CAR), a natural essential oil, after their surface immobilization onto gold (Au) substrates through a polydopamine (pDA) layer. The successful deposition and properties of each layer were characterized using ellipsometry, water contact angle (WCA) measurements, Fourier Transform Infrared Reflection-Absorption Spectroscopy (FT-IRRAS), X-ray Photoelectron Spectroscopy (XPS), and Atomic Force Microscopy (AFM). The resulting coatings displayed a thin, uniform film with smooth topography and with enhanced hydrophilicity. Antibacterial efficacy was assessed against <em>Staphylococcus epidermidis</em>, a relevant etiological agent in this context. The results revealed that the polydopamine-carvacrol coated surfaces (Au-pDA-CAR) exhibited a significant reduction in bacterial viability, achieving a 96 % decrease compared to unmodified gold surfaces. This was contrasted with minimal antibacterial activity from surfaces with either only polydopamine (Au-pDA) or carvacrol (Au-CAR). Live/Dead bacterial viability assays confirmed the bactericidal effect of the Au-pDA-CAR surface, demonstrating its effectiveness in killing bacteria rather than merely preventing adhesion. Our findings indicate that the pDA-CAR coating presents a promising approach for developing antimicrobial surfaces with enhanced performance against biofilm-forming pathogens. The development of this coating is an important step towards the establishment of a new technological platform capable of preventing medical device associated infections.</div></div>","PeriodicalId":279,"journal":{"name":"Colloids and Surfaces B: Biointerfaces","volume":"252 ","pages":"Article 114679"},"PeriodicalIF":5.4,"publicationDate":"2025-04-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143799880","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Unlocking the power of nanohybrids: A critical review on carbon nanomaterial-functionalized silver nanoparticles for advanced antimicrobial applications","authors":"Galal Magdy ,&nbsp;Rehab H. Elattar ,&nbsp;Randa A. Abdel Salam ,&nbsp;Ghada M. Hadad ,&nbsp;Asmaa Kamal El-Deen","doi":"10.1016/j.colsurfb.2025.114678","DOIUrl":"10.1016/j.colsurfb.2025.114678","url":null,"abstract":"<div><div>Over the last decades, nanotechnology has enabled the development of several inorganic nanoparticles with significant biocidal properties against diverse microorganisms. Silver nanoparticles (AgNPs) are among the most promising antimicrobial nanomaterials that have attracted substantial attention in various fields due to their low cost, low toxicity, biocompatibility, photo and chemical stability, easy preparation, high fluorescence, and tunability. Carbon nanomaterials (CNMs) are another appealing nanomaterial with antimicrobial qualities. While the antimicrobial efficacy of both AgNPs and CNMs is well-established, there is significant interest in the creation of CNMs/AgNPs hybrid materials for several applications due to their potential to exhibit synergistic bactericidal properties that surpass the yields of their components. This review represents a general overview of the different kinds, characterization techniques, synthesis processes, and antimicrobial activity of CNMs/AgNPs, along with an analysis of their benefits, drawbacks, and antimicrobial applications. Researchers and scientists interested in learning more about the potential of CNMs/AgNPs for advanced antimicrobial applications are likely to find this review to be a valuable resource.</div></div>","PeriodicalId":279,"journal":{"name":"Colloids and Surfaces B: Biointerfaces","volume":"252 ","pages":"Article 114678"},"PeriodicalIF":5.4,"publicationDate":"2025-04-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143799881","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Exudate management nonwovens loaded with Cirsium japonicum DC extract for enhanced hemostatic and wound healing","authors":"Liying Zou ,&nbsp;Jingjun Dong ,&nbsp;Ruyi Xie ,&nbsp;Aihua Cui ,&nbsp;Zunyuan Wang ,&nbsp;Guoxin Wang ,&nbsp;Jiwei Li ,&nbsp;Weichao Chen","doi":"10.1016/j.colsurfb.2025.114673","DOIUrl":"10.1016/j.colsurfb.2025.114673","url":null,"abstract":"<div><div>The chitosan-viscose-polyester composite nonwoven (CS-VIS-PET) was successfully prepared by needle-punching, which exhibited a unidirectional liquid transport property. Then, the extract of Cirsium japonicum DC (CJDC) was sprayed on the CS-layer of the CS-VIS-PET (CJDC@CS-VIS-PET) to improve the antibacterial, anti-oxidation, and hemostatic properties. With 10 % drug-loaded, the 10 %CJDC@CS-VIS-PET represented a high antioxidant rate (87.3 %), and the antibacterial rate against <em>E. coli</em> and <em>S. aureus</em> was 99.5 % and 99.7 %, respectively. Due to the synergistic mechanism of the blood capture effect of the CS-layer, the blood concentration effect of the VIS-layer, the blood expulsion effect of the PET-layer, and the chemical activation of CJDC, 10 %CJDC@CS-VIS-PET achieved excellent bleeding control ability. Moreover, the sandwich-structured 10 %CJDC@CS-VIS-PET showed excellent air permeability, water vapor permeability, and wound exudate management performance, which is beneficial for wound healing. The wound healing ratio on day 14 was as high as 92 %. In addition, profited from the excellent biocompatibility of chitosan, viscose and CJDC, 10 %CJDC@CS-VIS-PET exhibited outstanding blood and cell compatibility. Therefore, the sandwich-structured nonwovens loaded with the CJDC may provide a reference for developing multifunctional wound dressings with enhanced hemostatic and wound environment management properties.</div></div>","PeriodicalId":279,"journal":{"name":"Colloids and Surfaces B: Biointerfaces","volume":"252 ","pages":"Article 114673"},"PeriodicalIF":5.4,"publicationDate":"2025-04-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143792617","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Silica-coated gold nanorods conjugated with CRANAD to improve the detection of soluble β-amyloid species via a surface-enhanced fluorescence effect","authors":"María Paz Oyarzún ,&nbsp;Pablo Cabrera ,&nbsp;Nicole Parra-Muñoz ,&nbsp;Valentina López-Monsalves ,&nbsp;Ana Luisa Riveros ,&nbsp;Freddy Celis ,&nbsp;Raquel Gimeno-Muñoz ,&nbsp;Arántzazu González-Campo ,&nbsp;Núria Aliaga-Alcalde ,&nbsp;Mónica Soler ,&nbsp;Marcelo J. Kogan","doi":"10.1016/j.colsurfb.2025.114660","DOIUrl":"10.1016/j.colsurfb.2025.114660","url":null,"abstract":"<div><div>Alzheimer's disease (AD), one of the most common neurodegenerative disorders among the elderly, poses significant challenges due to limited therapeutic options and diagnostic complexity. The soluble and insoluble forms of β-amyloid (Aβ) peptide serve as disease biomarkers present in the early stages of the disease, highlighting the importance of developing detection methodologies. We designed a nanosystem utilizing silica-coated plasmonic nanoparticles linked to a CRANAD probe (AuNRs@SiO₂-CR2), which shows an improved fluorescence signal for the detection of Aβ through a surface enhancement effect. Our study identified an optimal thickness of the silica layer that results in enhanced fluorescence intensity. Furthermore, we observed a notable preferential detection towards the more toxic soluble Aβ species. In comparison to the free CRANAD, we achieved an approximately 800-fold increase in fluorescence signal, hence facilitating an improvement of the sensitivity for the detection of the Aβ aggregates. The designed nanomaterials will enhance the detection of soluble Aβ aggregates, which is crucial for the early diagnosis of Alzheimer´s disease.</div></div>","PeriodicalId":279,"journal":{"name":"Colloids and Surfaces B: Biointerfaces","volume":"252 ","pages":"Article 114660"},"PeriodicalIF":5.4,"publicationDate":"2025-04-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143829138","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Metal-organic frameworks-molecularly imprinted polymers (MOF-MIP): Synthesis, properties, and applications in detection and control of microorganisms","authors":"Yijie Kong,&nbsp;Yuhan Sun,&nbsp;Zhengrong Tian,&nbsp;Sha Liu,&nbsp;Ning Li","doi":"10.1016/j.colsurfb.2025.114670","DOIUrl":"10.1016/j.colsurfb.2025.114670","url":null,"abstract":"<div><div>Microbial contamination poses a significant threat to human health, food safety, and the ecological environment. Its rapid spread and potential pathogenicity create an urgent global challenge for efficient detection and control. However, existing methods have several shortcomings such as traditional techniques like culture methods and polymerase chain reaction (PCR) are time-consuming, while nanomaterials and aptamers often lack selectivity, stability, and affordability. Additionally, conventional disinfectants can be inefficient, lead to drug resistance, and harm the environment. To address these challenges, developing new materials and technologies that are efficient, sensitive, and stable is crucial for microbial detection and control. In this context, metal-organic frameworks (MOF) and molecularly imprinted polymers (MIP) have emerged as promising functional materials due to their unique structural advantages. The high porosity of MOF provides ample imprinting sites for MIP, while MIP enhance selective adsorption and inactivation of target microorganisms by MOF. This synergistic combination results in a composite material that offers a novel solution for microbial detection, significantly improving sensitivity, selectivity, antibacterial efficiency, and environmental friendliness. This paper reviews the synthesis strategies of metal-organic frameworks-molecularly imprinted polymers (MOF-MIP), highlighting their structural properties and innovative applications in microbial detection, which aim to inspire researchers in related fields. Looking ahead, future advancements in material science and biotechnology are expected to lead to widespread use of MOF-MIP composites in food safety, environmental monitoring, medical diagnosis, and public health—providing robust support against microbial pollution. By studying the collaborative mechanisms of MOF and MIP while optimizing design processes will enhance precision speed cost-effectiveness in microbial detection technology significantly contributing to human health and environmental safety.</div></div>","PeriodicalId":279,"journal":{"name":"Colloids and Surfaces B: Biointerfaces","volume":"252 ","pages":"Article 114670"},"PeriodicalIF":5.4,"publicationDate":"2025-04-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143816897","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Imaging probes for the detection of brain microenvironment","authors":"Shiming Zhang ,&nbsp;Tianyu Wang ,&nbsp;Tianzi Gao ,&nbsp;Jun Liao ,&nbsp;Yang Wang ,&nbsp;Meng Xu ,&nbsp;Changyu Lu ,&nbsp;Jianfeng Liang ,&nbsp;Zhengren Xu ,&nbsp;Jianfei Sun ,&nbsp;Qian Xie ,&nbsp;Zhiqiang Lin ,&nbsp;Hongbin Han","doi":"10.1016/j.colsurfb.2025.114677","DOIUrl":"10.1016/j.colsurfb.2025.114677","url":null,"abstract":"<div><div>The brain microenvironment (BME) is a highly dynamic system that plays a critical role in neural excitation, signal transmission, development, aging, and neurological disorders. BME consists of three key components: neural cells, extracellular spaces, and physical fields, which provide structures and physicochemical properties to synergistically and antagonistically regulate cell behaviors and functions such as nutrient transport, waste metabolism and intercellular communication. Consequently, monitoring the BME is vital to acquire a better understanding of the maintenance of neural homeostasis and the mechanisms underlying neurological diseases. In recent years, researchers have developed a range of imaging probes designed to detect changes in the microenvironment, enabling precise measurements of structural and biophysical parameters in the brain. This advancement aids in the development of improved diagnostic and therapeutic strategies for brain disorders and in the exploration of cutting-edge mechanisms in neuroscience. This review summarizes and highlights recent advances in the probes for sensing and imaging BME. Also, we discuss the design principles, types, applications, challenges, and future directions of probes.</div></div>","PeriodicalId":279,"journal":{"name":"Colloids and Surfaces B: Biointerfaces","volume":"252 ","pages":"Article 114677"},"PeriodicalIF":5.4,"publicationDate":"2025-04-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143816898","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Osteoimmunomodulation unveiled: Enhancing bone regeneration with 3D-printed PLLA/β-TCP/CS scaffolds","authors":"Chaode Cen ,&nbsp;Chuan Wang ,&nbsp;Yong Zhang ,&nbsp;Chaoran Hu ,&nbsp;Lingli Tang ,&nbsp;Chengwei Liu ,&nbsp;Yongfei Cao ,&nbsp;Tao Wang ,&nbsp;Wuxun Peng","doi":"10.1016/j.colsurfb.2025.114674","DOIUrl":"10.1016/j.colsurfb.2025.114674","url":null,"abstract":"<div><div>Bone tissue engineering shows great potential for repairing large segmental bone defects; however, the immune response to biological scaffolds remains a critical factor influencing bone regeneration. Despite this, there is a paucity of studies investigating the effects of biomaterial components and their degradation products on macrophage polarization and the subsequent process of bone regeneration. This study investigates the role of macrophages in osteogenesis and angiogenesis induced by PLLA(Poly-L-Lactic Acid)/β-TCP(β-Tricalcium Phosphate)/CS(Calcium Sulfate) bone scaffolds in vitro and in vivo. Various PLLA/β-TCP/CS scaffolds were fabricated via 3D printing and characterized for their physicochemical properties. The effect of P/T15/S15 (PLLA/β-TCP/CS scaffold containing 15 % β-TCP and 15 % CS) on macrophage polarization and the secretion of VEGF and BMP-2 was assessed in vitro. Additionally, the conditioned medium derived from macrophages stimulated with P/T15/S15 was evaluated for its effects on the migration and osteogenic differentiation of bone marrow-derived stem cells (BMSCs), as well as the angiogenic potential of human umbilical vein endothelial cells (HUVECs). In vivo, the relationship between macrophage polarization and osteogenesis was examined in a rabbit tibia segmental defect model. The results demonstrated that the 3D-printed P/T15/S15 scaffold exhibited favorable physicochemical properties and compatibility with BMSCs and RAW264.7 macrophages, while not disrupting BMSC apoptosis. P/T15/S15 promoted polarization of M0 macrophages towards the M2 phenotype, resulting in an increased secretion of the anti-inflammatory cytokine IL-10, as well as growth factors such as VEGF and BMP-2. However, it did not suppress TNF-α levels. Additional, the conditioned medium derived from P/T15/S15-stimulated macrophages significantly enhanced the osteogenesis of BMSCs, their migration, and the angiogenesis of HUVECs. In the rabbit model, P/T15/S15 facilitated bone regeneration by promoting macrophage polarization towards the M2 phenotype and reducing inflammation. This study highlights that P/T15/S15 regulates macrophage polarization, enhances osteogenesis and angiogenesis, and positions itself as a promising candidate for bone tissue engineering through osteoimmunomodulation.</div></div>","PeriodicalId":279,"journal":{"name":"Colloids and Surfaces B: Biointerfaces","volume":"252 ","pages":"Article 114674"},"PeriodicalIF":5.4,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143768119","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}