Colloids and Surfaces B: Biointerfaces最新文献_第8页

Influence of coordination number and ionic radius on metal ion preference and activity of lanthanide-dependent alcohol dehydrogenase: Insights from mutational studies and density functional theory

IF 5.4 2区医学

Colloids and Surfaces B: Biointerfaces Pub Date : 2025-02-25 DOI: 10.1016/j.colsurfb.2025.114596

Lun Wang , Ke Liu , Zhongdi Song , Hainam Do , Lirong Yang , Jianping Wu , Ling Jiang , Haoran Yu

{"title":"Influence of coordination number and ionic radius on metal ion preference and activity of lanthanide-dependent alcohol dehydrogenase: Insights from mutational studies and density functional theory","authors":"Lun Wang , Ke Liu , Zhongdi Song , Hainam Do , Lirong Yang , Jianping Wu , Ling Jiang , Haoran Yu","doi":"10.1016/j.colsurfb.2025.114596","DOIUrl":"10.1016/j.colsurfb.2025.114596","url":null,"abstract":"<div><div>Lanthanide (Ln) elements form a cofactor complex with pyrroloquinoline quinone (PQQ) in bacterial alcohol dehydrogenases (Ln<sup>3 +</sup>-ADH). The lanthanide elements did not support Ln<sup>3+</sup>-ADH activity equally, with only early lanthanides (La<sup>3+</sup>-Gd<sup>3+</sup>) promoting high enzyme activity. However, the early lanthanides did not promote the activity equally and the detailed mechanism of Ln<sup>3+</sup>-ADH exhibiting different activity in the presence of different light Lns remains obscure. To uncover the role of lanthanides in promoting Ln<sup>3+</sup>-ADH activity, we systemically characterized the activity of an Ln<sup>3+</sup>-ADH from <em>Pseudomonas putida</em> KT2440 (PedH) in the presence of various Ln<sup>3+</sup> ions. In the results, enzyme activity displayed a bell-shaped trend along with the lanthanide series, with Nd<sup>3+</sup> providing the highest activity. Active site mutation analysis revealed that modifying the number of coordinating ligands shifted the metal preference of the enzyme. DFT calculation revealed that the HOMO-LUMO gap, substrate interaction energy and metal ions binding distances were critical for the lanthanides in promoting enzyme activity. This work shed light on the critical role of metal ions in Ln<sup>3+</sup>-ADH catalysis, providing insights for future exploration and engineering of Ln-dependent proteins.</div></div>","PeriodicalId":279,"journal":{"name":"Colloids and Surfaces B: Biointerfaces","volume":"251 ","pages":"Article 114596"},"PeriodicalIF":5.4,"publicationDate":"2025-02-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143527521","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Biomaterial-associated infections: Their development, characterization, prevention and treatment

IF 5.4 2区医学

Colloids and Surfaces B: Biointerfaces Pub Date : 2025-02-25 DOI: 10.1016/j.colsurfb.2025.114595

Theerthankar Das , Reza Nejadnik , Virginia Vadillo Rodríguez , Yong Liu , Guruprakash Subbiahdoss

{"title":"Biomaterial-associated infections: Their development, characterization, prevention and treatment","authors":"Theerthankar Das , Reza Nejadnik , Virginia Vadillo Rodríguez , Yong Liu , Guruprakash Subbiahdoss","doi":"10.1016/j.colsurfb.2025.114595","DOIUrl":"10.1016/j.colsurfb.2025.114595","url":null,"abstract":"<div><div>Biomaterials including catheters, artificial joints, dental implants, and contact lenses are highly susceptible to bacterial adhesion, colonization, and biofilm formation, leading to biomaterials-associated infections (BAIs). BAIs pose a significant challenge to the healthcare sector, contributing to increased morbidity, mortality, and economic burden. Preventing bacterial adhesion and biofilm formation is crucial to eliminate BAIs. This special issue, dedicated to Prof. Henk J. Busscher (The University of Groningen, The Netherlands) on the occasion of his retirement, presents original research showcasing innovative strategies to combat biofilm formation on biomaterial surfaces and prevent BAIs. Notable approaches include graphene oxide-copper combinational coatings on cotton fabrics, which exhibited a significant reduction, in <em>Staphylococcus aureus</em> and <em>Pseudomonas aeru</em>ginosa adhesion. Another study demonstrated vancomycin functionalized zwitterionic polymer brush coatings showed antimicrobial and antiadhesive properties against <em>Staphylococcus aureus</em> and <em>Escherichia coli</em> biofilms. Additionally, photokilling-based prevention of bacterial colonization emerges as a promising strategy. Importantly, this special issue also underscores the limitations of mono-culture models in biomaterial testing and highlights the necessity of <em>in vitro</em> co-culture assays integrating bacterial and mammalian cells for a more physiologically relevant evaluation of BAIs.</div></div>","PeriodicalId":279,"journal":{"name":"Colloids and Surfaces B: Biointerfaces","volume":"251 ","pages":"Article 114595"},"PeriodicalIF":5.4,"publicationDate":"2025-02-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143527462","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Designing a T cell multi-epitope vaccine against hRSV with reverse vaccinology: An immunoinformatics approach

IF 5.4 2区医学

Colloids and Surfaces B: Biointerfaces Pub Date : 2025-02-25 DOI: 10.1016/j.colsurfb.2025.114599

Peibin Guan , Congyan Qi , Guojin Xu , Can Sheng , Siqi Sun , Zhicheng Zhou , Shulei Jia

{"title":"Designing a T cell multi-epitope vaccine against hRSV with reverse vaccinology: An immunoinformatics approach","authors":"Peibin Guan , Congyan Qi , Guojin Xu , Can Sheng , Siqi Sun , Zhicheng Zhou , Shulei Jia","doi":"10.1016/j.colsurfb.2025.114599","DOIUrl":"10.1016/j.colsurfb.2025.114599","url":null,"abstract":"<div><div>As an infectious viral pathogen, human respiratory syncytial virus (hRSV) can cause severe respiratory infections and is recognized as one of the highest priority pathogens by the World Health Organization (WHO). Although vaccines play an important role in disease prevention and transmission, the wild-type virus is usually prone to immune escape due to the relatively high mutation rate of biological proteins. Therefore, designing a broad-spectrum hRSV vaccine is essential to provide extensive protection against multiple viral variants. Using a consensus sequence approach, we designed a broad-spectrum T-cell epitope vaccine composed of 385 amino acids, consisting of 12 CTLs and 5 HTLs from the fusion protein and glycoprotein. The designed multi-epitope vaccine was expected to have non-allergenicity, high population coverage, strong antigenicity and immunogenicity, appropriate physical and chemical properties, and high solubility. Meanwhile, the structure of the vaccine had a high similarity to that of the natural virus. In addition, through structural biology analysis, the constructed vaccine achieved robust structural compactness and binding stability. Computer-generated immunological simulations indicated that the vaccine could elicit realistic immune responses in humans. The designed vaccine showed good binding affinity and molecular and immune simulation. In conclusion, the broad-spectrum hRSV vaccine could be an excellent candidate for preventing hRSV infection. The employed prediction pipeline was proved to be an efficient method for screening immunogenic epitopes of additional pathogens.</div></div>","PeriodicalId":279,"journal":{"name":"Colloids and Surfaces B: Biointerfaces","volume":"251 ","pages":"Article 114599"},"PeriodicalIF":5.4,"publicationDate":"2025-02-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143527520","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Screening lipid nanoparticles using DNA barcoding and qPCR

IF 5.4 2区医学

Colloids and Surfaces B: Biointerfaces Pub Date : 2025-02-25 DOI: 10.1016/j.colsurfb.2025.114598

Dawei Liu , Xing Wang , Letao Xu , Zahraa Nima Saeed Al-Delfi , Zelalem Addis Mekonnen , Song Gao , Branka Grubor-Bauk , Chun-Xia Zhao

{"title":"Screening lipid nanoparticles using DNA barcoding and qPCR","authors":"Dawei Liu , Xing Wang , Letao Xu , Zahraa Nima Saeed Al-Delfi , Zelalem Addis Mekonnen , Song Gao , Branka Grubor-Bauk , Chun-Xia Zhao","doi":"10.1016/j.colsurfb.2025.114598","DOIUrl":"10.1016/j.colsurfb.2025.114598","url":null,"abstract":"<div><div>Quantifying the biodistribution of lipid nanoparticles (LNPs) is critical for optimizing mRNA delivery systems, yet current approaches have inherent limitations. This study introduces a cost-effective method utilizing double-stranded DNA (dsDNA) barcodes and quantitative polymerase chain reaction (qPCR) for rapid analysis of a small library of mRNA-LNPs biodistribution and functional delivery <em>in vivo</em>. Three unique 100-bp dsDNA barcodes were designed to represent for three FDA-approved LNP formulations. Concurrently, these three formulations carrying luciferase mRNA were mixed with DNA-barcoding LNPs as a pool. Following intravenous administration of the pooled LNPs in mice, qPCR analysis revealed the highest abundance of DNA barcodes and accumulation of luciferase mRNA in spleen, with positive correlation between barcodes presence and mRNA localization across organs, validating DNA barcodes as reliable indicators of mRNA-LNPs biodistribution <em>in vivo</em>. Bioluminescence imaging further confirmed successful delivery and protein translation of luciferase mRNA facilitated by the LNPs <em>in vivo</em>. Integrating DNA barcodes for biodistribution analysis and luciferase mRNA for assessing functional delivery enabled comprehensive evaluation of LNP performance. This robust methodology provides valuable insights into the localization patterns and mRNA delivery capabilities of different LNP formulations, paving the way for the development of more effective and targeted mRNA-based therapeutics.</div></div>","PeriodicalId":279,"journal":{"name":"Colloids and Surfaces B: Biointerfaces","volume":"251 ","pages":"Article 114598"},"PeriodicalIF":5.4,"publicationDate":"2025-02-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143521317","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Editorial for Special Issue “Biomaterial-associated infections: Their development, characterization, prevention and treatment”

IF 5.4 2区医学

Colloids and Surfaces B: Biointerfaces Pub Date : 2025-02-23 DOI: 10.1016/j.colsurfb.2025.114594

Theerthankar Das, Reza Nejadnik, Virginia Vadillo Rodríguez, Yong. Liu, Guruprakash Subbiahdoss

引用次数: 0

Enzyme mimics based on self-assembled peptide functionalized with graphene oxide for polyethylene terephthalate degradation

IF 5.4 2区医学

Colloids and Surfaces B: Biointerfaces Pub Date : 2025-02-21 DOI: 10.1016/j.colsurfb.2025.114588

Xia Li , Yaoling Zhou , Jingchao Yue , Mengyu Sun , Xiangmin Lei , Piwu Li , Jianpeng Li , Dengyue Sun , Zhixiong Zeng

{"title":"Enzyme mimics based on self-assembled peptide functionalized with graphene oxide for polyethylene terephthalate degradation","authors":"Xia Li , Yaoling Zhou , Jingchao Yue , Mengyu Sun , Xiangmin Lei , Piwu Li , Jianpeng Li , Dengyue Sun , Zhixiong Zeng","doi":"10.1016/j.colsurfb.2025.114588","DOIUrl":"10.1016/j.colsurfb.2025.114588","url":null,"abstract":"<div><div>The degradation of polyethylene terephthalate (PET) has garnered notable attention owing to its widespread accumulation and the challenges associated with its breakdown. Herein, the enzyme mimics with PET-hydrolytic activity were developed by combining peptide nanofibers with graphene oxide (GO). Inspired by native enzymes, we designed self-assembled peptides that included active amino acids (serine, histidine, aspartate and tryptophan) and different hydrophobic amino acids, with a 9-fluorenylmethoxycarbonyl group at the N-terminus. Our comparison of hydrophobic amino acids revealed that their content not only influenced the higher-order assembly of peptide but also affected molecular conformation and PET degradation ability. By co-assembling two peptides with catalytic and binding sites in a 1:1 ratio, a more effective active enzyme mimic was constructed which was owning to the cooperative interactions among the active amino acids; in addition, hydrogen bonds and π-π stacking interactions were the main forces in enhancing catalytic effects. To further improve PET-hydrolytic ability, the co-assembled enzyme mimic was functionalised with GO through π–π stacking. This GO-peptide nanofiber hybrid exhibited increased PET-hydrolytic, as GO provided a hydrophobic microenvironment for substrate attraction and abundant carbon for facilitating proton transfer. The GO-peptide nanofiber hybrid as enzyme mimics will be a promising material for PET degradation.</div></div>","PeriodicalId":279,"journal":{"name":"Colloids and Surfaces B: Biointerfaces","volume":"251 ","pages":"Article 114588"},"PeriodicalIF":5.4,"publicationDate":"2025-02-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143488430","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Influence of consortium culture and mixed culture on carbon steel corrosion in B30 storage system

IF 5.4 2区医学

Colloids and Surfaces B: Biointerfaces Pub Date : 2025-02-21 DOI: 10.1016/j.colsurfb.2025.114587

Ardiyan Harimawan, Hary Devianto, Byan Baihaqi, Nilam Khairon Nisa, Christian Aslan

{"title":"Influence of consortium culture and mixed culture on carbon steel corrosion in B30 storage system","authors":"Ardiyan Harimawan, Hary Devianto, Byan Baihaqi, Nilam Khairon Nisa, Christian Aslan","doi":"10.1016/j.colsurfb.2025.114587","DOIUrl":"10.1016/j.colsurfb.2025.114587","url":null,"abstract":"<div><div>B30, which consisted of 30 %-v biodiesel and 70 %-v petrodiesel, is a renewable fuel that is being developed in Indonesia. In the B30 storage system, microbes utilize B30 and cause corrosion of carbon steel. This research aims to compare the interaction effect of consortium culture (<em>S. marcescens</em> – <em>B. megaterium</em> and <em>S. marcescens</em> – <em>B. licheniformis</em>) and mixed culture on the corrosion of carbon steel in the B30 storage system. The experiment was carried out by immersing carbon steel ST-37 specimens in B30 test medium for 21 days. Sample testing and analysis includes the number of microbial colonies, chemical bonds of biofilm composition, morphology of biofilms and metal surfaces, corrosion rate and corrosion products. The results shows that antagonistic interactions occurred in the consortium culture, resulting in the decrease of corrosion rate. Meanwhile, synergistic interaction occurred between the microbes in the mixed culture, resulting in higher corrosion rate. The corrosion mechanism that occurs in consortium culture and mixed culture involves the same electrochemical reactions.</div></div>","PeriodicalId":279,"journal":{"name":"Colloids and Surfaces B: Biointerfaces","volume":"251 ","pages":"Article 114587"},"PeriodicalIF":5.4,"publicationDate":"2025-02-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143521316","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Prolonged local retention of vancomycin achieved by a multivesicular liposomes in thermoresponsive gel system for the prevention and treatment of intervertebral disc infection

IF 5.4 2区医学

Colloids and Surfaces B: Biointerfaces Pub Date : 2025-02-21 DOI: 10.1016/j.colsurfb.2025.114593

Zhencheng Gao , Hongxia Fan , Lutong He , Yu Zhang , Tian Yin , Haibing He , Xing Tang , Yanjiao Wang , Jingxin Gou

{"title":"Prolonged local retention of vancomycin achieved by a multivesicular liposomes in thermoresponsive gel system for the prevention and treatment of intervertebral disc infection","authors":"Zhencheng Gao , Hongxia Fan , Lutong He , Yu Zhang , Tian Yin , Haibing He , Xing Tang , Yanjiao Wang , Jingxin Gou","doi":"10.1016/j.colsurfb.2025.114593","DOIUrl":"10.1016/j.colsurfb.2025.114593","url":null,"abstract":"<div><div>The therapeutic efficacy of intervertebral disc (IVD) infections treated with intravenous vancomycin (VCM) is often limited by inadequate blood supply to the IVD. In this study, we developed a localized and sustained-release drug delivery system for the intradiscal administration of VCM. First, VCM-loaded multivesicular liposomes (VCM-MVLs) were prepared using a two-step emulsification process, and we investigated the effects of the preparation process and formulation composition on the quality of the MVLs. The prepared MVLs exhibited an encapsulation efficiency of 92.08 ± 6.44 %, a particle size of 30.35 μm, and a sustained release over 12 days. Subsequently, VCM-MVLs were incorporated with PLGA-PEG-PLGA (PPP) to create a thermosensitive hydrogel composite formulation (VCM-MVL-PPP), which demonstrated a gelling temperature of 28.4°C and exhibited prolonged sustained release over 20 days. The antimicrobial activity of VCM-MVL-PPP was maintained for an extended duration of 21 days. Additionally, the VCM-MVL-PPP demonstrated an optimal retention capacity of 14 days in the <em>in vivo</em> retention assay. <em>In vivo</em> safety results confirmed the high biocompatibility and negligible toxicity of the formulation. In conclusion, the presence of PPP as a secondary release barrier for the drug released from MVLs allowed VCM-MVL-PPP to exhibit prolonged drug release and retention in the IVDs compared to VCM-MVLs. Our findings provide a valuable reference for peptide delivery and the treatment of disc infections.</div></div>","PeriodicalId":279,"journal":{"name":"Colloids and Surfaces B: Biointerfaces","volume":"251 ","pages":"Article 114593"},"PeriodicalIF":5.4,"publicationDate":"2025-02-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143488429","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Selenium nanoparticles as catalysts for nitric oxide generation

IF 5.4 2区医学

Colloids and Surfaces B: Biointerfaces Pub Date : 2025-02-20 DOI: 10.1016/j.colsurfb.2025.114592

Shu Geng , Yingzhu Zhou , Gervase Ng , Qingqing Fan , Soshan Cheong , Federico Mazur , Cyrille Boyer , Rona Chandrawati

{"title":"Selenium nanoparticles as catalysts for nitric oxide generation","authors":"Shu Geng , Yingzhu Zhou , Gervase Ng , Qingqing Fan , Soshan Cheong , Federico Mazur , Cyrille Boyer , Rona Chandrawati","doi":"10.1016/j.colsurfb.2025.114592","DOIUrl":"10.1016/j.colsurfb.2025.114592","url":null,"abstract":"<div><div>The critical role of nitric oxide (NO), a potent signalling molecule, in various physiological processes has driven the development of NO delivery strategies for numerous therapeutic applications. However, NO’s short half-life poses a significant challenge for its effective delivery. Glutathione peroxidase, a selenium-containing antioxidant enzyme, can catalyse the decomposition of <em>S</em>-nitrosothiols (endogenous NO prodrugs) to produce NO <em>in situ</em>. Inspired by this, we explored selenium nanoparticles (SeNPs) for their enzyme-mimicking NO-generating activity. Stabilised with polyvinyl alcohol (PVA) or chitosan (CTS), SeNPs demonstrated tuneable NO generation when exposed to varying concentrations of NO prodrug, nanoparticles, and glutathione (GSH). In the presence of GSH, a naturally occurring antioxidant in the human body, 0.1 µg mL<sup>−1</sup> of SeNPs could catalytically generate 7.5 µM of NO under physiological conditions within 30 min. We investigated the effects of nanoparticle crystallinity and NO prodrug type on NO generation, as well as the stability and sustained NO generation of the catalytic nanoparticles. PVA-stabilised SeNPs were non-toxic to NIH 3T3 cells and effectively dispersed <em>Pseudomonas aeruginosa</em> biofilms upon NO generation. This study broadens the repertoire of nanomaterials for NO generation and highlights SeNPs as a non-toxic alternative for therapeutic NO delivery.</div></div>","PeriodicalId":279,"journal":{"name":"Colloids and Surfaces B: Biointerfaces","volume":"251 ","pages":"Article 114592"},"PeriodicalIF":5.4,"publicationDate":"2025-02-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143527461","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Oral functional protein Z: Mitigation of thrombosis via thrombin inhibition to prevent cardiovascular disease

IF 5.4 2区医学

Colloids and Surfaces B: Biointerfaces Pub Date : 2025-02-20 DOI: 10.1016/j.colsurfb.2025.114569

Chen Xu , Hanhan Liu , Mingyang Sun, Yang Gao, Tuo Zhang, Guanghua Zhao, Chenyan Lv

{"title":"Oral functional protein Z: Mitigation of thrombosis via thrombin inhibition to prevent cardiovascular disease","authors":"Chen Xu , Hanhan Liu , Mingyang Sun, Yang Gao, Tuo Zhang, Guanghua Zhao, Chenyan Lv","doi":"10.1016/j.colsurfb.2025.114569","DOIUrl":"10.1016/j.colsurfb.2025.114569","url":null,"abstract":"<div><div>Thrombin, a serine protease, plays a crucial role in thrombosis and is considered a significant target for the treatment of thrombotic diseases. This study aimed to investigate the malt-derived serine protease inhibitor protein Z (PZ) as an oral food enrichment for inhibiting thrombosis. The ability of PZ to withstand gastrointestinal digestion was assessed through in vitro simulated gastrointestinal digestion experiments. Its effective ability to traverse the digestive tract was demonstrated in vivo experiments. To explore its antithrombotic mechanism, the antithrombin activity of PZ was evaluated using two thrombin substrates, suggesting that it may inhibit either the active site or exosite 1 of thrombin. Additionally, the half-life of PZ was 10.76 ± 0.45 min, indicating its favorable pharmacokinetic profile. The anticoagulant activity and antithrombotic effects of PZ were further assessed using a mice tail thrombosis model induced by κ-carrageenan. The black tail rate in the PZ group is 51.23 ± 1.72 % lower than that of the model group (71.87 ± 5.90 %). These results demonstrated that PZ significantly inhibited thrombosis, with its physiological mechanism linked to the coagulation pathway, particularly through the inhibition of thrombin activity. Therefore, PZ has the potential to be developed as an oral antithrombotic food enrichment.</div></div>","PeriodicalId":279,"journal":{"name":"Colloids and Surfaces B: Biointerfaces","volume":"251 ","pages":"Article 114569"},"PeriodicalIF":5.4,"publicationDate":"2025-02-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143478749","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0