Colloids and Surfaces B: Biointerfaces最新文献_第7页

Oxygen self-supplied BTOv-Ru heterojunction boosts the sonodynamic antibacterial efficiency of scaffold 氧自供BTOv-Ru异质结提高了支架的声动力抗菌效率

IF 5.6 2区医学

Colloids and Surfaces B: Biointerfaces Pub Date : 2025-09-20 DOI: 10.1016/j.colsurfb.2025.115153

Wenjie Ma , Ru Jia , Huixing Li , Lanlan Dong , Wurikaixi Aiyiti , Cijun Shuai , Chongxian He

{"title":"Oxygen self-supplied BTOv-Ru heterojunction boosts the sonodynamic antibacterial efficiency of scaffold","authors":"Wenjie Ma , Ru Jia , Huixing Li , Lanlan Dong , Wurikaixi Aiyiti , Cijun Shuai , Chongxian He","doi":"10.1016/j.colsurfb.2025.115153","DOIUrl":"10.1016/j.colsurfb.2025.115153","url":null,"abstract":"<div><div>Transplantation-related bacterial infections often slow new tissue regeneration and even cause transplant failure. Sonodynamic therapy is a promising antibacterial method, which kills bacteria by triggering sonosensitizers to generate reactive oxygen species (ROS). Nevertheless, the generation efficiency of ROS is hindered by the fast electron-hole pair recombination in sonosensitizers and the hypoxic of bacteria microenvironment. Herein, ruthenium nanoparticles (Ru NPs) are grown in-situ on the oxygen vacancies-rich barium titanate nanoparticles (BTOv NPs) to form a Schottky heterojunction. The resulting BTOv-Ru NPs as sonosensitizers is uniformly distributed with poly-L-lactic acid (PLLA) powders to fabricate ultrasound-responsive scaffolds via selective laser sintering. On the one hand, the Schottky heterojunction facilitates electron injection from BTOv to Ru, while the resultant charge separation significantly suppresses electron-hole recombination. On the other hand, the catalase-like activity of Ru catalyzes the overexpressed hydrogen peroxide to generate O<sub>2</sub> to achieve O<sub>2</sub> self-supply. This not only alleviates hypoxia but also facilitates the oxygen vacancies of BTOv adsorb more O<sub>2</sub> to generate ROS. The results confirmed PLLA/BTOv-Ru scaffold generated numerous ROS and exhibited excellent antibacterial effects under ultrasonic irradiation, with inhibiting <em>E. coli</em> (89.3 %) and <em>S. aureus</em> (88.1 %). This O<sub>2</sub> self-supplying sonodynamic therapy shows great promise in antibacterial treatment.</div></div>","PeriodicalId":279,"journal":{"name":"Colloids and Surfaces B: Biointerfaces","volume":"257 ","pages":"Article 115153"},"PeriodicalIF":5.6,"publicationDate":"2025-09-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145106503","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Macrophage-targeted amphiphilic peptide nanocarrier for intracellular MRSA infection therapy 巨噬细胞靶向两亲肽纳米载体治疗细胞内MRSA感染

IF 5.6 2区医学

Colloids and Surfaces B: Biointerfaces Pub Date : 2025-09-19 DOI: 10.1016/j.colsurfb.2025.115151

Zaipeng Chen , Yuling Wu , Zhiqiang Nie , Tengfei Mao , Junjun Tao , Changming Tang , Huajun Ruan , Xin Lang , Wei Zhou , Jiaju Lu , Xigong Li

{"title":"Macrophage-targeted amphiphilic peptide nanocarrier for intracellular MRSA infection therapy","authors":"Zaipeng Chen , Yuling Wu , Zhiqiang Nie , Tengfei Mao , Junjun Tao , Changming Tang , Huajun Ruan , Xin Lang , Wei Zhou , Jiaju Lu , Xigong Li","doi":"10.1016/j.colsurfb.2025.115151","DOIUrl":"10.1016/j.colsurfb.2025.115151","url":null,"abstract":"<div><div>Intracellular bacterial infections pose a significant challenge due to limited cellular internalization, low intracellular antibiotic efficacy, and bacterial sequestration within specific cellular compartments. In this study, we designed an amphiphilic peptide drug delivery system (RFP@TVYV) for sequential targeting of macrophages and intracellular bacteria eradication. By modifying the macrophage-targeting molecule tuftsin, RFP@TVYV facilitates rapid internalization by macrophages. Additionally, the incorporation of Val-Cit fragments, responsive to lysosomal cathepsin B, enables the controlled release of the cell-penetrating peptide YGRKKRRQRRR (TAT) and rifampin (RFP). The inclusion of TAT further enhances subcellular targeting, directing RFP to the bacterial cytoplasm to effectively disrupt intracellular pathogens. Synthesis and characterization studies confirmed that RFP@TVYV self-assembles into stable nanomicelles through hydrogen bonding and hydrophobic interactions. Antibacterial assays demonstrated the nanoplatform’s potent activity against <em>Staphylococcus aureus</em>, while flow cytometry (FC) and immunofluorescence (IF) confirmed increased macrophage uptake efficiency and intracellular targeting. Both in <em>vitro</em> and in <em>vivo</em> studies showed that RFP@TVYV significantly reduces intracellular bacterial load more effectively than free RFP, with minimal cytotoxicity. These findings underscore the potential of RFP@TVYV as an advanced drug delivery platform for combating intracellular bacterial infections, particularly those caused by drug-resistant pathogens such as methicillin-resistant <em>Staphylococcus aureus</em> (MRSA).</div></div>","PeriodicalId":279,"journal":{"name":"Colloids and Surfaces B: Biointerfaces","volume":"257 ","pages":"Article 115151"},"PeriodicalIF":5.6,"publicationDate":"2025-09-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145106501","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

DMSO-mediated reversible disassembly-reassembly of ferritin nanocages for efficient encapsulation and glioma-targeted delivery of hydrophobic drug ddmso介导的铁蛋白纳米笼的可逆拆卸-重组，用于高效包封和胶质瘤靶向递送疏水药物

IF 5.6 2区医学

Colloids and Surfaces B: Biointerfaces Pub Date : 2025-09-18 DOI: 10.1016/j.colsurfb.2025.115149

Huangtao Xu , YuanYuan Shao , Haining Xia , Tianwei Song , Changhao Wang , Shuai Xu , Sajid ur Rehman , Zehua Li , Ruiguo Chen , Jing Zhang , Junchao Qian , Kun Ma , Yongxing Pan , Junfeng Wang

{"title":"DMSO-mediated reversible disassembly-reassembly of ferritin nanocages for efficient encapsulation and glioma-targeted delivery of hydrophobic drug","authors":"Huangtao Xu , YuanYuan Shao , Haining Xia , Tianwei Song , Changhao Wang , Shuai Xu , Sajid ur Rehman , Zehua Li , Ruiguo Chen , Jing Zhang , Junchao Qian , Kun Ma , Yongxing Pan , Junfeng Wang","doi":"10.1016/j.colsurfb.2025.115149","DOIUrl":"10.1016/j.colsurfb.2025.115149","url":null,"abstract":"<div><div>Ferritin, as a highly promising protein nanocage, has been widely applied in the fields of vaccines, nanoparticle synthesis, drug delivery, and so forth. Recently, ferritin-based drug delivery has been made remarkable progress, nevertheless, enhancing drug loading efficiency and yield still desired. Here, a novel dis/reassembly method for ferritin drugs loading is proposed using dimethyl sulfoxide (DMSO). Briefly, dried HFn (H-ferritin) is dissolved with DMSO to disrupt its cage structures, then the disassembled HFn is diluted in HEPES to reassemble into the 24-mer cage, while simultaneously could encage chemotherapeutic drugs inside the cage. The results shown that this disassembly-reassembly method achieves a recovery rate of HFn exceeding 80 % and a drug loading capacity of 65.3 Dox per cage, surpassing previously reported disassembly-reassembly methods. Importantly, the new prepared HFn-Dox can target tumor <em>in vitro</em> and <em>in vivo</em>. And the anticancer effects of HFn-Dox also has been evaluated with in situ glioma using both MR imaging and <em>in vivo</em> fluorescence imaging methods, founding their superior tumor growth inhibition in comparison with free Dox. Thus, the DMSO-mediated dis/reassembly drug loading method of ferritin provides a new potential approach to the preparation of ferritin-based drugs for tumor diagnosis and therapy.</div></div>","PeriodicalId":279,"journal":{"name":"Colloids and Surfaces B: Biointerfaces","volume":"257 ","pages":"Article 115149"},"PeriodicalIF":5.6,"publicationDate":"2025-09-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145106575","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Multi-functional poly(N-isopropylacrylamide)-based hydrogel with hemostasis, antibacterial, and sustained drug release properties for infected wound treatment 多功能聚（n -异丙基丙烯酰胺）基水凝胶具有止血、抗菌和药物缓释特性，用于感染伤口治疗。

IF 5.6 2区医学

Colloids and Surfaces B: Biointerfaces Pub Date : 2025-09-18 DOI: 10.1016/j.colsurfb.2025.115147

Tao Gong , Ruolan Feng , Huirui Zhu , Moxuan Zhao , Baofeng Yu , Na Wang , Hongqin Wang , Rui Guo

引用次数: 0

Dual-responsive magnetic nanozyme Cu-CuFe₂O₄ leverages mild magnetic hyperthermia and redox dyshomeostasis to potentiate cuproptosis 双响应磁纳米酶Cu-CuFe₂O₄利用轻度磁热疗和氧化还原失衡来增强铜还原。

IF 5.6 2区医学

Colloids and Surfaces B: Biointerfaces Pub Date : 2025-09-17 DOI: 10.1016/j.colsurfb.2025.115146

Yanzhao Diao , Shuaibo Yang , Hong Qu , Laiping Fang , Wangzi Liu , Wei Guo , Wenjing Li , Meng Zhang , Jin Fang , Yudan Du , Lifeng Hang , Hong Li , Guihua Jiang

{"title":"Dual-responsive magnetic nanozyme Cu-CuFe₂O₄ leverages mild magnetic hyperthermia and redox dyshomeostasis to potentiate cuproptosis","authors":"Yanzhao Diao , Shuaibo Yang , Hong Qu , Laiping Fang , Wangzi Liu , Wei Guo , Wenjing Li , Meng Zhang , Jin Fang , Yudan Du , Lifeng Hang , Hong Li , Guihua Jiang","doi":"10.1016/j.colsurfb.2025.115146","DOIUrl":"10.1016/j.colsurfb.2025.115146","url":null,"abstract":"<div><div>Cuproptosis, a copper-dependent programmed cell death pathway, has shown limited antitumor efficacy due to the tumor responsiveness and copper bioavailability. To address these challenges, we engineered hyaluronic acid functionalized Cu-CuFe₂O₄ nanoparticles (CCIO@HA NPs) that enhance cuproptosis therapy through synergistic exploitation of mild magnetic hyperthermia therapy (MMHT)-induced mitochondrial copper overload and multiple enzyme activities-driven redox dyshomeostasis (RDH). Specifically, the system ensures precise copper accumulation to initiate cuproptosis by coupling pH/MMHT triggered Cu²⁺ release. Simultaneously, CCIO@HA NPs induce RDH <em>via</em> multiple enzyme activities: glutathione oxidase (GSHOx)-like activity depletes GSH to inhibit copper chelation, thereby amplifying mitochondrial copper overload; catalase (CAT)-like activity generates O₂ to alleviate hypoxia, which enhances mitochondrial respiration-driven cuproptosis; and peroxidase (POD)/Fenton like-derived activity produces hydroxyl radical (·OH), driving irreversible RDH. Crucially, MMHT synergizes with RDH to degrade heat shock protein 70 (HSP70), thereby sensitizing tumors to copper cytotoxicity. Furthermore, CCIO@HA NPs enable precise photoacoustic/magnetic resonance imaging-guided therapy. <em>In vitro</em> and <em>in vivo</em> experiments demonstrate an excellent tumor growth inhibition with high biocompatibility. This work establishes a paradigm-shifting strategy that coordinate MMHT with RDH, effectively addressing the dual bottlenecks of cuproptosis in solid tumor therapy.</div></div>","PeriodicalId":279,"journal":{"name":"Colloids and Surfaces B: Biointerfaces","volume":"257 ","pages":"Article 115146"},"PeriodicalIF":5.6,"publicationDate":"2025-09-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145102505","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Surface-engineered MnO₂@Au@ZIF-67 hierarchical catalysts for acid-triggered antibacterial therapy and infected wound healing 表面工程mno2 @Au@ZIF-67分级催化剂用于酸触发的抗菌治疗和感染伤口愈合。

IF 5.6 2区医学

Colloids and Surfaces B: Biointerfaces Pub Date : 2025-09-16 DOI: 10.1016/j.colsurfb.2025.115145

Mengmeng Xu , Yuan Liu , Zhe Tang , Xiaolin Yu , Li Gao , Bo Yang , Yan Wang , Xuehui Dong , Qianqian Yu , LinGe Wang

{"title":"Surface-engineered MnO₂@Au@ZIF-67 hierarchical catalysts for acid-triggered antibacterial therapy and infected wound healing","authors":"Mengmeng Xu , Yuan Liu , Zhe Tang , Xiaolin Yu , Li Gao , Bo Yang , Yan Wang , Xuehui Dong , Qianqian Yu , LinGe Wang","doi":"10.1016/j.colsurfb.2025.115145","DOIUrl":"10.1016/j.colsurfb.2025.115145","url":null,"abstract":"<div><div>Rational surface engineering of nanocatalysts offers new solutions against antibiotic-resistant infections. We designed and fabricated hierarchical MnO₂@Au@ZIF-67 nanoparticles (NPs) with triple interfacial functionalities for synergistic antibacterial therapy. The acid-responsive ZIF-67 surface degrades in infected wounds, releasing Co²⁺ to catalyze •OH generation via Fenton-like reactions at the material-bacteria interface. Simultaneously, ultrasmall Au surfaces deplete glucose through oxidase-mimetic catalysis, producing gluconic acid that accelerates ZIF-67 dissolution. The MnO₂ core surface decomposes H₂O₂ to supply O₂, enhancing Au catalysis and alleviating hypoxia. Comprehensive surface characterization (TEM, SEM, XPS, XRD) confirmed structural integrity and reactive sites. <em>In vitro</em>, the NPs achieved > 99 % bacterial killing <em>(S. aureus/E. coli</em>) through ROS-mediated membrane disruption (validated by SEM/CLSM). <em>In vivo</em>, they enabled 98.45 % bacterial clearance and accelerated wound healing via collagen reorganization (H&E/Masson staining). This surface-engineered platform demonstrates how interfacial catalytic cascades can be harnessed for effective non-antibiotic antimicrobial applications.</div></div>","PeriodicalId":279,"journal":{"name":"Colloids and Surfaces B: Biointerfaces","volume":"257 ","pages":"Article 115145"},"PeriodicalIF":5.6,"publicationDate":"2025-09-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145090930","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Tailoring V-Mo dual active sites nanozymes-induced oxidative damage of bacteria for anti-infection therapy 裁剪V-Mo双活性位点纳米酶诱导细菌氧化损伤的抗感染治疗。

IF 5.6 2区医学

Colloids and Surfaces B: Biointerfaces Pub Date : 2025-09-16 DOI: 10.1016/j.colsurfb.2025.115143

Yuhan Sun , Yizhou Yang , Fan Wu , Anjing Ren , Ao Li

{"title":"Tailoring V-Mo dual active sites nanozymes-induced oxidative damage of bacteria for anti-infection therapy","authors":"Yuhan Sun , Yizhou Yang , Fan Wu , Anjing Ren , Ao Li","doi":"10.1016/j.colsurfb.2025.115143","DOIUrl":"10.1016/j.colsurfb.2025.115143","url":null,"abstract":"<div><div>Bacterial infections continue to pose a serious global health threat, further intensified by the rapid rise of antimicrobial resistance. Addressing this urgent challenge, nanozymes—nanomaterials with intrinsic enzyme-like catalytic properties—have gained attention as innovative agents in antimicrobial therapy. Molybdenum disulfide (MoS<sub>2</sub>) nanozymes exhibit peroxidase (POD)-like activity, favorable biocompatibility, and strong near-infrared (NIR) absorption, making them highly suitable for applications in photothermal therapy (PTT) and chemodynamic therapy (CDT). However, their relatively low catalytic efficiency significantly limits their practical utility. In this work, we successfully developed vanadium-doped MoS<sub>2</sub> nanozymes (VDMSNz), which integrate a dual-site cooperative catalytic mechanism to enhance the antibacterial effect. The introduction of vanadium dopants induces local charge redistribution, which significantly enhances the nanozymes catalytic capabilities. In this system, Mo active sites serve as substrate-binding centers, facilitating the near-barrierless dissociation of H<sub>2</sub>O<sub>2</sub>, while V substitution sites exhibit favorable binding characteristics that promote OH* desorption and the subsequent generation of •OH radicals. Importantly, both <em>in vitro</em> and <em>in vivo</em> experiments have demonstrated that VDMSNz-induced CDT and PTT exhibit potent antibacterial activity against methicillin-resistant <em>Staphylococcus aureus</em> (<em>MRSA</em>) and <em>Escherichia coli</em> (<em>E. coli</em>). These findings underscore the potential of VDMSNz as effective therapeutic agents for combating resistant bacterial infections.</div></div>","PeriodicalId":279,"journal":{"name":"Colloids and Surfaces B: Biointerfaces","volume":"257 ","pages":"Article 115143"},"PeriodicalIF":5.6,"publicationDate":"2025-09-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145102480","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Construction and evaluation of a long-circulating thermosensitive liposomal black phosphorus quantum dot delivery system for osteosarcoma therapy 用于骨肉瘤治疗的长循环热敏脂质体黑磷量子点递送系统的构建与评价。

IF 5.6 2区医学

Colloids and Surfaces B: Biointerfaces Pub Date : 2025-09-16 DOI: 10.1016/j.colsurfb.2025.115144

Zhou Zhang , Jun-yi Yang , Jia-han Chen , Hua Zhang , Wen-bin Zhao , Yong-sheng Li , Feng Yu , Wen Chen

{"title":"Construction and evaluation of a long-circulating thermosensitive liposomal black phosphorus quantum dot delivery system for osteosarcoma therapy","authors":"Zhou Zhang , Jun-yi Yang , Jia-han Chen , Hua Zhang , Wen-bin Zhao , Yong-sheng Li , Feng Yu , Wen Chen","doi":"10.1016/j.colsurfb.2025.115144","DOIUrl":"10.1016/j.colsurfb.2025.115144","url":null,"abstract":"<div><div>Bone malignant tumors are diseases that endanger human lives and compromise patients' quality of life. Tumors are conventionally treated via chemotherapy; however, nonspecific damage to healthy cells and inefficient targeting of tumor sites often occur, necessitating the development of an innovative treatment paradigm. In this study, a long-circulating thermosensitive liposome encapsulating DOX-loaded BPQDs was constructed to release drugs via pH/NIR response for photothermal-chemotherapy synergistic treatment of osteosarcoma. Initially, BPQDs were synthesized through liquid-phase exfoliation coupled with a solvothermal reaction. Subsequently, DOX was immobilized onto the BPQD surface via electrostatic interactions to ensure efficient chemotherapeutic drug loading. Ultimately, the high-performance Lip-BPQDs-DOX drug delivery system was fabricated by encapsulating BPQDs-DOX within long-circulating thermosensitive liposomes using a thin-film dispersion-extrusion method. The drug delivery system demonstrates a transition in size from large to small, superior photothermal conversion efficiency, pH/NIR-responsive drug release, favorable ex vivo and in vivo biocompatibility, and liposome-mediated, photothermal-chemotherapeutic synergistic anti-tumor effects. These characteristics offer valuable insights for designing multifunctional nanotherapeutic systems for related tumors like osteosarcoma.</div></div>","PeriodicalId":279,"journal":{"name":"Colloids and Surfaces B: Biointerfaces","volume":"257 ","pages":"Article 115144"},"PeriodicalIF":5.6,"publicationDate":"2025-09-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145090925","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Superoxide dismutase-mimetic nanozymes: A promising alternative to natural superoxide dismutases for biomedical and industrial applications 超氧化物歧化酶模拟纳米酶：生物医学和工业应用中天然超氧化物歧化酶的有前途的替代品

IF 5.6 2区医学

Colloids and Surfaces B: Biointerfaces Pub Date : 2025-09-16 DOI: 10.1016/j.colsurfb.2025.115138

Hoda Zamanian Dastmalchi, Fariba Dashtestani, Hedayatollah Ghourchian

{"title":"Superoxide dismutase-mimetic nanozymes: A promising alternative to natural superoxide dismutases for biomedical and industrial applications","authors":"Hoda Zamanian Dastmalchi, Fariba Dashtestani, Hedayatollah Ghourchian","doi":"10.1016/j.colsurfb.2025.115138","DOIUrl":"10.1016/j.colsurfb.2025.115138","url":null,"abstract":"<div><div>Superoxide dismutases (SODs) are vital metalloenzymes that catalyze the dismutation of superoxide radicals, thereby shielding cells from oxidative damage. Natural SODs have many obstacles to overcome despite their potential for treatment and diagnosis, such as high production costs, instability, and vulnerability to inactivation in harsh environments. With their improved stability, adjustable catalytic activity, and affordability, synthetic SOD nanozymes have become a viable substitute. The redox-active metal centers and substrate-binding microenvironments of natural SODs, which are essential for effective O<sub>2</sub><sup>•-</sup> scavenging, are modeled in the design of these nanozymes. Notwithstanding their benefits, SOD nanozymes still need to be further optimized to overcome drawbacks like complexity of synthesis, catalytic specificity, biocompatibility, and activity enhancement. Customized designs for industrial and biomedical applications are made possible by precise control over nanozymes' properties made possible by advanced fabrication techniques (such as chemical vapor deposition and hydrothermal synthesis). Clarifying catalytic mechanisms, improving synthesis procedures, and creating individualized treatment plans should be the main goals of future research. SOD nanozymes have the potential to completely transform oxidative stress management in industrial, environmental, and medical settings with further development. Finally, the final viewpoint is taken into consideration, along with the difficulties and potential paths for using SOD-mimetic nanozymes in research or business.</div></div>","PeriodicalId":279,"journal":{"name":"Colloids and Surfaces B: Biointerfaces","volume":"257 ","pages":"Article 115138"},"PeriodicalIF":5.6,"publicationDate":"2025-09-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145106504","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Multivalent capture of circulating tumor cells using tetrahedral DNA framework-targeted nanomagnetic beads integrated microfluidic device 四面体DNA框架靶向纳米磁珠集成微流控装置在循环肿瘤细胞多价捕获中的应用。

IF 5.6 2区医学

Colloids and Surfaces B: Biointerfaces Pub Date : 2025-09-15 DOI: 10.1016/j.colsurfb.2025.115142

Chuang Zhang , Xinhua Zhang , Jian Zhang , Mian Peng , Xiaowen Dou

{"title":"Multivalent capture of circulating tumor cells using tetrahedral DNA framework-targeted nanomagnetic beads integrated microfluidic device","authors":"Chuang Zhang , Xinhua Zhang , Jian Zhang , Mian Peng , Xiaowen Dou","doi":"10.1016/j.colsurfb.2025.115142","DOIUrl":"10.1016/j.colsurfb.2025.115142","url":null,"abstract":"<div><div>Circulating tumor cells (CTCs), though rare and highly heterogeneous, are closely associated with tumor initiation, progression, and prognosis. Their low abundance, structural fragility, phenotypic diversity, and size variability make efficient enrichment particularly challenging. To address these limitations, we developed a novel CTC isolation platform that combines tetrahedral DNA nanostructure-based trivalent aptamer magnetic nanospheres (TDF-Cocktail-MNP) with a magnetic microfluidic separation device. This system enhances the capture of viable, phenotypically diverse CTCs and supports reliable downstream analysis. The TDF structure presents three aptamers: EpCAM targets epithelial CTCs, Vimentin captures mesenchymal types, and EGFR binds to cells overexpressing EGFR—a key marker in triple-negative breast cancer. This multi-target approach enables efficient isolation of heterogeneous CTC populations from whole blood samples. Captured CTCs are enriched using the IsoFlux System, which reduces mechanical stress and preserves cell viability by avoiding repeated magnetic manipulation. After nuclease-mediated removal of the DNA scaffold, cells are classified and quantified through immunocytochemistry. Validated with both spiked and clinical breast cancer samples, this platform shows strong potential for liquid biopsy applications, offering robust CTC detection and valuable insights for personalized cancer diagnostics and treatment.</div></div>","PeriodicalId":279,"journal":{"name":"Colloids and Surfaces B: Biointerfaces","volume":"257 ","pages":"Article 115142"},"PeriodicalIF":5.6,"publicationDate":"2025-09-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145090923","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0