Colloids and Surfaces B: Biointerfaces最新文献

{"title":"Near-infrared light-responsive copper-cerium bimetallic oxide nanozyme with antibacterial and antioxidant abilities for periodontitis therapy","authors":"Xiaowen Li ,&nbsp;Hao Liang ,&nbsp;Yonghui Huang ,&nbsp;Qiurui Hu ,&nbsp;Lingling Liang ,&nbsp;Jingchuan He ,&nbsp;Qinglan Lu ,&nbsp;Guangqi Zhou ,&nbsp;Li Chen ,&nbsp;Cuiping Li ,&nbsp;Xiaojie Li","doi":"10.1016/j.colsurfb.2025.114685","DOIUrl":"10.1016/j.colsurfb.2025.114685","url":null,"abstract":"<div><div>Periodontitis, a chronic inflammatory disease of the supporting tissue around teeth, is triggered by periodontal pathogens. Clinical treatments face the problem of bacterial resistance and most therapies focus on a single function, lacking the multifunctional treatment of antibacterial, antioxidant, anti-inflammatory, and osteogenic properties. In our study, we developed a copper-cerium bimetallic oxide (CuCeO_x) nanozyme with near-infrared (NIR) light responsiveness for the periodontitis therapy. Under the excitation of 808 nm NIR light, CuCeO_x displayed excellent photodynamic and photothermal activities, efficiently generating reactive oxygen species (ROS) and heat. After the treatment of the CuCeO_x/NIR system, the inhibition rate of <em>Porphyromonas gingivalis</em> (<em>P. gingivalis</em>), the main periodontitis pathogen, reached 98.69 ± 0.23 % in vitro. Without the NIR light irradiation, CuCeO_x, acting as a nanozyme, exhibited enzyme-like activity in scavenging ROS, effectively alleviating the cellular oxidative stress. Furthermore, CuCeO_x significantly mitigated the cellular inflammatory response induced by lipopolysaccharide (LPS) and promoted osteogenesis under the oxidative stress condition. Notably, the CuCeO_x exhibited excellent blood compatibility (hemolysis &lt; 5 %). The efficacy of the CuCeO_x/NIR system in vivo was also investigated. H&amp;E staining results demonstrated a significant reduction in periodontal tissue inflammation following treatment. Micro-CT analysis revealed that CuCeO_x effectively inhibited the alveolar bone loss. Additionally, we found CuCeO_x regulated the Nrf2/HO-1 signaling pathway both in vitro and in vivo. In conclusion, the multifunctional nanomaterial CuCeO_x provides a promising strategy for the treatment of periodontitis.</div></div>","PeriodicalId":279,"journal":{"name":"Colloids and Surfaces B: Biointerfaces","volume":"252 ","pages":"Article 114685"},"PeriodicalIF":5.4,"publicationDate":"2025-04-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143829156","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Nano-selenium alleviated immunoresponse, apoptosis and oxidative stress in Leydig cells of yak","authors":"Hao Li ,&nbsp;Yujun Tang ,&nbsp;Hui Wang ,&nbsp;Xinyue Liu ,&nbsp;Yutian Zeng ,&nbsp;Run Zhang ,&nbsp;Cuiting Yang ,&nbsp;Arab Khan ,&nbsp;Bing Wu ,&nbsp;Xianxiang Wang ,&nbsp;Ming Zhang","doi":"10.1016/j.colsurfb.2025.114684","DOIUrl":"10.1016/j.colsurfb.2025.114684","url":null,"abstract":"<div><div>Nano-selenium(SENP) plays a crucial role in maintaining cellular redox homeostasis and serves as an antioxidant in cell culture medium. This study investigated the cytoprotective effects of SENP against lipopolysaccharide (LPS)-induced toxicity in yak Leydig cells. In this research, in vitro cultured Leydig cells were exposed to LPS to simulate Gram-negative bacterial infection. Following LPS induction, the cell apoptosis rate reached 28 %, with significant increases in inflammation and oxidative stress markers including IL-6, IL-8, MDA, and ROS. Concurrently, testosterone concentration decreased by nearly 60 %. Subsequently, SENP was introduced into the culture medium. We then evaluated apoptosis, oxidative stress, immune response, and testosterone concentration in Leydig cells. The results demonstrated that SENP effectively protected Leydig cells from LPS-induced damage.</div></div>","PeriodicalId":279,"journal":{"name":"Colloids and Surfaces B: Biointerfaces","volume":"252 ","pages":"Article 114684"},"PeriodicalIF":5.4,"publicationDate":"2025-04-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143821005","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Nanoparticles of quercetin-gellan gum conjugate for the controlled delivery of fluconazole","authors":"Azka Fatima ,&nbsp;Shahid Shah ,&nbsp;Akhtar Rasul ,&nbsp;Ghulam Abbas ,&nbsp;Abid Mahmood ,&nbsp;Muhammad Hanif ,&nbsp;Alina Ilyas ,&nbsp;Maira Tahir ,&nbsp;Safirah Maheen ,&nbsp;Iqra Mubeen ,&nbsp;Tahira Zaheer ,&nbsp;Muhammad Abu Sufian ,&nbsp;Saleha Babar ,&nbsp;Nader I. Namazi","doi":"10.1016/j.colsurfb.2025.114681","DOIUrl":"10.1016/j.colsurfb.2025.114681","url":null,"abstract":"<div><div>The aim of the study was to formulate the polymeric nanoparticles for the controlled delivery of fluconazole (FCZ) to enhance its absorption, mucosal adherence, and bioavailability. FCZ-loaded gellan gum-quercetin conjugate (GQC) nanoparticles (FCZ-loaded NGQ) were prepared by ionic gelatin method using GQC, surfactants, co-surfactants, and cross-linker. The nanoparticles were then characterized by various physical and chemical analytical tools such as ¹H NMR, FTIR, XRD, DSC, TGA, particle size, zeta potential, and SEM analysis. All the nanoparticles have a nanosized range from 108 nm to 123 nm with a zeta potential of −12 mV to −20 mV which shows their stability. The NGQ3 (optimized formulation) loaded with FCZ have a PDI of 0.20 ± 0.02 and % entrapment efficiency of 32.3 ± 2.8 %. These studies also showed promising results of 85 % drug release, the value for zero order kinetics of <em>R</em>^<em>2</em> was 0.999, mucosal adherence of 24 h, and the highest values of permeation was shown by NGQ3 of 60 %. The improved antifungal activity of prepared NGQ3-DL against <em>Candida albicans</em> was observed compared to the pure drug. The nanoparticles may offer enhanced bioavailability and controlled release of FCZ, making them a potential breakthrough in managing FCZ resistant infections.</div></div>","PeriodicalId":279,"journal":{"name":"Colloids and Surfaces B: Biointerfaces","volume":"252 ","pages":"Article 114681"},"PeriodicalIF":5.4,"publicationDate":"2025-04-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143825546","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Synthetic selenomelanin nanoparticles radio-sensitize non-melanocytic lung cancer (A549) cells by promoting G2/M arrest","authors":"J. Aishwarya ,&nbsp;Ram Pada Das ,&nbsp;Atanu Barik ,&nbsp;Amit Kunwar","doi":"10.1016/j.colsurfb.2025.114680","DOIUrl":"10.1016/j.colsurfb.2025.114680","url":null,"abstract":"<div><div>Recent studies have postulated the natural existence of selenomelanin and its role in the radio-protection of healthy cells. The present study aimed to understand its radio-modulatory activity in non-melanocytic cancerous (A549) cells of lung origin. Briefly, selenomelanin was synthesized under laboratory conditions following the previously reported methodology. The various spectroscopic (electron paramagnetic resonance, X-ray photoelectron spectroscopy, atomic absorption spectroscopy, transmission electron microscopy and dynamic light scattering) analyses confirmed the formation of selenomelanin nanoparticles. The short-term (72 h) and long-term (14 days) toxicity profiling of selenomelanin by 3-[4,5-dimethylthiazol-2-yl]-2,5 diphenyl tetrazolium bromide (MTT) and clonogenic assays respectively revealed its half maximal inhibitory concentrations (IC₅₀) of 72.03 ± 7.13 μg/ml and 0.85 ± 0.16 μg/ml respectively in A549 cells and of 81.56 ± 1.63 μg/ml and &gt; 5 μg/ml respectively in healthy lung fibroblast (WI26) cells. Further, pre-treatment of selenomelanin (at concentrations non-toxic for WI26 cells) selectively augmented the radiosensitivity of A549 cells. Finally, mechanistic investigations in A549 cells revealed that selenomelanin increased the levels of reactive oxygen species, DNA damage and modulated the phospho-levels of CHK1 and CHK2 (effectors of cell cycle arrest) in the irradiated cells to favour G2/M arrest followed by cleavage of caspase 3 (effector of apoptosis). Together, the present study proposes the novel application of selenomelanin as a radiosensitizer to enhance the efficacy of radiotherapy in cancerous cells of lung origin.</div></div>","PeriodicalId":279,"journal":{"name":"Colloids and Surfaces B: Biointerfaces","volume":"252 ","pages":"Article 114680"},"PeriodicalIF":5.4,"publicationDate":"2025-04-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143838965","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"PEGylated PAMAM dendrimer nanoplatform for co-delivery of chemotherapeutic agents and inorganic nanoparticles enhancing chemo-photothermal combination therapy","authors":"Binzhong Lu ,&nbsp;Yingying Yang ,&nbsp;Xiang Li ,&nbsp;Qiuli Cheng ,&nbsp;Leitao Zhang ,&nbsp;Wenlan Wu ,&nbsp;Junbo Li","doi":"10.1016/j.colsurfb.2025.114688","DOIUrl":"10.1016/j.colsurfb.2025.114688","url":null,"abstract":"<div><div>Chemo-photothermal combination therapy has emerged as an important approach for enhancing therapeutic efficacy against tumors. However, developing a flexible nanoplatform capable of co-encapsulating inorganic photothermal agents (PTAs) and organic antitumor drugs remains challenging. A polyethylene glycol-functionalized polyamidoamine (PAMAM) dendrimer (PAMAM-PEG) served as a template for the synthesis of copper sulfide (CuS) nanoparticles and subsequent encapsulation of doxorubicin (DOX) within its inner cavities. The multifunctional nanoplatform demonstrated high colloidal stability along with photothermal conversion efficiency upon 980 nm laser irradiation. This synergistic effect substantially improved DOX cellular uptake and tumor penetration, resulting in superior antitumor efficacy relative to chemotherapy alone. These results demonstrate that PAMAM-PEG represents a promising nanoplatform for combined chemo-photothermal therapy, providing a novel strategy to address current limitations in tumor treatment while enhancing therapeutic outcomes.</div></div>","PeriodicalId":279,"journal":{"name":"Colloids and Surfaces B: Biointerfaces","volume":"252 ","pages":"Article 114688"},"PeriodicalIF":5.4,"publicationDate":"2025-04-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143820925","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Treatment of ulcerative colitis by sustained release curcumin pellets coated with ethyl ascorbic acid; in-vitro, in-vivo, and in-silico analysis","authors":"Dure Shahwar ,&nbsp;Muhammad Hanif ,&nbsp;Qasim Umar ,&nbsp;Muhammad Rafiq ,&nbsp;Khalid Mahmood ,&nbsp;Muhammad Qaiser ,&nbsp;Nabeela Ameer ,&nbsp;Muhammad Haseeb llyas","doi":"10.1016/j.colsurfb.2025.114671","DOIUrl":"10.1016/j.colsurfb.2025.114671","url":null,"abstract":"<div><div>The present work aimed to protect the rapid reduction of Curcumin (Cur) in alkaline pH by newly synthesized ethyl ascorbic acid (EAA) in dual-coated Cur pellets (CCur-P4d) used for the treatment of inflammatory bowel disease (IBD). Physicochemical and morphological analysis was performed by FTIR, DSC, XRD, and SEM-EDS and in vitro release study. 92.87 ± 6.26 % release of Cur from uncoated Cur pellets (UCur-P4) in 0.1 M HCl at pH 1.2 was controlled to 5 % by coating with Eudragit L-100 (EL-100) which interns increased to 97.99 ± 7.35 % in 100 mM phosphate buffer of pH 7.4. The scavenging activity of 2,2-Diphenyl-1-picrylhydrazyl (DPPH) and hydrogen peroxides (H₂O₂) was 95.88 ± 1.22 % and 85.35 ± 1.43 %, respectively. Acute toxicity study and anti-ulcerative effect of CCur-P4d in ulcerative colitis (UC) induced albino rats were evaluated by microscopic and macroscopic analysis. In protein-ligand binding interaction, Cur showed a potent inhibitory effect on inflammatory markers C-reactive protein, IL-6, and TNF-α. Conclusively, CCur-P4d is considered an alternative to locally acting therapeutics agents used in IBD.</div></div>","PeriodicalId":279,"journal":{"name":"Colloids and Surfaces B: Biointerfaces","volume":"252 ","pages":"Article 114671"},"PeriodicalIF":5.4,"publicationDate":"2025-04-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143824431","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Enhanced cancer therapy using modified magnetic α-Fe2O3/Fe3O4 nanorods: Dual role in curcumin delivery and ferroptosis induction","authors":"Mingyi Ma ,&nbsp;Zhongjun Pan ,&nbsp;Ziye Zhu ,&nbsp;Chen Ling ,&nbsp;Jiahao Yuan ,&nbsp;Xiangdong Huo ,&nbsp;Shasha Li ,&nbsp;Ruijiang Liu","doi":"10.1016/j.colsurfb.2025.114689","DOIUrl":"10.1016/j.colsurfb.2025.114689","url":null,"abstract":"<div><div>Curcumin (CUR) has gained considerable attention in oncology due to its potent anti-tumor, anti-inflammatory, and antioxidant properties. However, its clinical utility was significantly hindered by low bioavailability and rapid metabolic degradation. This work designed a magnetic α-Fe₂O₃/Fe₃O₄ heterogeneous nanorod prepared via a urea hydrolysis-calcination process for curcumin delivery. The nanorods were modified with hyaluronic acid (HA), providing a stable matrix for curcumin encapsulation. The zeta potential of α-Fe₂O₃/Fe₃O₄/HA/CUR was −3.94 mV, the saturation magnetization was 7.82 emu·g⁻¹, the encapsulation rate and drug loading rate were 10.53 % and 29.64 % respectively. At pH 5.4, 6.5, and 7.4, the release rates were 44.8 %, 40.2 %, and 39.3 %, respectively. Kinetic modeling indicated that release profiles followed the Weibull kinetic model, with an R² value greater than 0.98. The α-Fe₂O₃/Fe₃O₄/HA/CUR exhibited excellent magnetic responsiveness, demonstrating a significant inhibitory effect on human liver cancer (HepG2) cells (inhibition rate greater than 60 %) under a magnetic field. Moreover, they substantially reduced the cytotoxicity of curcumin on normal human (LO2) cells. The α-Fe₂O₃/Fe₃O₄/HA/CUR inhibited migration and proliferation of HepG2 cells, induced apoptosis via the Caspase pathway, and synergistically suppressed tumor cell development through ferroptosis. The drug release from the α-Fe₂O₃/Fe₃O₄/HA/CUR was stable, significantly enhancing the bioavailability of curcumin. This provided a promising strategy for improving the bioavailability of poorly soluble drugs and enhancing liver cancer treatment outcomes.</div></div>","PeriodicalId":279,"journal":{"name":"Colloids and Surfaces B: Biointerfaces","volume":"252 ","pages":"Article 114689"},"PeriodicalIF":5.4,"publicationDate":"2025-04-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143824432","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Hybrid structure combining essential oil derivatives and polydopamine for anti-bacterial coatings","authors":"Nathan Jourdainne ,&nbsp;Dimitri Mercier ,&nbsp;Bruna Costa ,&nbsp;Filipa Campos ,&nbsp;Claudia Monteiro ,&nbsp;Natália Costa ,&nbsp;M. Cristina L. Martins ,&nbsp;Pascal Thébault","doi":"10.1016/j.colsurfb.2025.114679","DOIUrl":"10.1016/j.colsurfb.2025.114679","url":null,"abstract":"<div><div>The development of effective antimicrobial surfaces is crucial for reducing the risk of medical device-associated infections. This study investigates the antibacterial potential of carvacrol (CAR), a natural essential oil, after their surface immobilization onto gold (Au) substrates through a polydopamine (pDA) layer. The successful deposition and properties of each layer were characterized using ellipsometry, water contact angle (WCA) measurements, Fourier Transform Infrared Reflection-Absorption Spectroscopy (FT-IRRAS), X-ray Photoelectron Spectroscopy (XPS), and Atomic Force Microscopy (AFM). The resulting coatings displayed a thin, uniform film with smooth topography and with enhanced hydrophilicity. Antibacterial efficacy was assessed against <em>Staphylococcus epidermidis</em>, a relevant etiological agent in this context. The results revealed that the polydopamine-carvacrol coated surfaces (Au-pDA-CAR) exhibited a significant reduction in bacterial viability, achieving a 96 % decrease compared to unmodified gold surfaces. This was contrasted with minimal antibacterial activity from surfaces with either only polydopamine (Au-pDA) or carvacrol (Au-CAR). Live/Dead bacterial viability assays confirmed the bactericidal effect of the Au-pDA-CAR surface, demonstrating its effectiveness in killing bacteria rather than merely preventing adhesion. Our findings indicate that the pDA-CAR coating presents a promising approach for developing antimicrobial surfaces with enhanced performance against biofilm-forming pathogens. The development of this coating is an important step towards the establishment of a new technological platform capable of preventing medical device associated infections.</div></div>","PeriodicalId":279,"journal":{"name":"Colloids and Surfaces B: Biointerfaces","volume":"252 ","pages":"Article 114679"},"PeriodicalIF":5.4,"publicationDate":"2025-04-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143799880","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Unlocking the power of nanohybrids: A critical review on carbon nanomaterial-functionalized silver nanoparticles for advanced antimicrobial applications","authors":"Galal Magdy ,&nbsp;Rehab H. Elattar ,&nbsp;Randa A. Abdel Salam ,&nbsp;Ghada M. Hadad ,&nbsp;Asmaa Kamal El-Deen","doi":"10.1016/j.colsurfb.2025.114678","DOIUrl":"10.1016/j.colsurfb.2025.114678","url":null,"abstract":"<div><div>Over the last decades, nanotechnology has enabled the development of several inorganic nanoparticles with significant biocidal properties against diverse microorganisms. Silver nanoparticles (AgNPs) are among the most promising antimicrobial nanomaterials that have attracted substantial attention in various fields due to their low cost, low toxicity, biocompatibility, photo and chemical stability, easy preparation, high fluorescence, and tunability. Carbon nanomaterials (CNMs) are another appealing nanomaterial with antimicrobial qualities. While the antimicrobial efficacy of both AgNPs and CNMs is well-established, there is significant interest in the creation of CNMs/AgNPs hybrid materials for several applications due to their potential to exhibit synergistic bactericidal properties that surpass the yields of their components. This review represents a general overview of the different kinds, characterization techniques, synthesis processes, and antimicrobial activity of CNMs/AgNPs, along with an analysis of their benefits, drawbacks, and antimicrobial applications. Researchers and scientists interested in learning more about the potential of CNMs/AgNPs for advanced antimicrobial applications are likely to find this review to be a valuable resource.</div></div>","PeriodicalId":279,"journal":{"name":"Colloids and Surfaces B: Biointerfaces","volume":"252 ","pages":"Article 114678"},"PeriodicalIF":5.4,"publicationDate":"2025-04-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143799881","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Exudate management nonwovens loaded with Cirsium japonicum DC extract for enhanced hemostatic and wound healing","authors":"Liying Zou ,&nbsp;Jingjun Dong ,&nbsp;Ruyi Xie ,&nbsp;Aihua Cui ,&nbsp;Zunyuan Wang ,&nbsp;Guoxin Wang ,&nbsp;Jiwei Li ,&nbsp;Weichao Chen","doi":"10.1016/j.colsurfb.2025.114673","DOIUrl":"10.1016/j.colsurfb.2025.114673","url":null,"abstract":"<div><div>The chitosan-viscose-polyester composite nonwoven (CS-VIS-PET) was successfully prepared by needle-punching, which exhibited a unidirectional liquid transport property. Then, the extract of Cirsium japonicum DC (CJDC) was sprayed on the CS-layer of the CS-VIS-PET (CJDC@CS-VIS-PET) to improve the antibacterial, anti-oxidation, and hemostatic properties. With 10 % drug-loaded, the 10 %CJDC@CS-VIS-PET represented a high antioxidant rate (87.3 %), and the antibacterial rate against <em>E. coli</em> and <em>S. aureus</em> was 99.5 % and 99.7 %, respectively. Due to the synergistic mechanism of the blood capture effect of the CS-layer, the blood concentration effect of the VIS-layer, the blood expulsion effect of the PET-layer, and the chemical activation of CJDC, 10 %CJDC@CS-VIS-PET achieved excellent bleeding control ability. Moreover, the sandwich-structured 10 %CJDC@CS-VIS-PET showed excellent air permeability, water vapor permeability, and wound exudate management performance, which is beneficial for wound healing. The wound healing ratio on day 14 was as high as 92 %. In addition, profited from the excellent biocompatibility of chitosan, viscose and CJDC, 10 %CJDC@CS-VIS-PET exhibited outstanding blood and cell compatibility. Therefore, the sandwich-structured nonwovens loaded with the CJDC may provide a reference for developing multifunctional wound dressings with enhanced hemostatic and wound environment management properties.</div></div>","PeriodicalId":279,"journal":{"name":"Colloids and Surfaces B: Biointerfaces","volume":"252 ","pages":"Article 114673"},"PeriodicalIF":5.4,"publicationDate":"2025-04-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143792617","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}