抗坏血酸乙酯包被姜黄素缓释微丸治疗溃疡性结肠炎体外，体内和计算机分析

IF 5.4 2区医学 Q1 BIOPHYSICS

Colloids and Surfaces B: Biointerfaces Pub Date : 2025-04-08 DOI:10.1016/j.colsurfb.2025.114671

Dure Shahwar , Muhammad Hanif , Qasim Umar , Muhammad Rafiq , Khalid Mahmood , Muhammad Qaiser , Nabeela Ameer , Muhammad Haseeb llyas

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引用次数: 0

摘要

本研究旨在保护新合成的乙基抗坏血酸（EAA）在治疗炎症性肠病（IBD）的双包被Cur微球（ccr - p4d）中的姜黄素（Cur）在碱性pH下的快速还原。采用FTIR、DSC、XRD、SEM-EDS进行理化、形态分析，并进行体外释放研究。用Eudragit L-100 （EL-100）包衣后，在pH为7.4的100 mM磷酸盐缓冲液中，未包衣的cuur - p4在0.1 M HCl中释放量为92.87 ± 6.26 %，在pH为1.2的100 M HCl中释放量为97.99 ± 7.35 %。对2,2-二苯基-1-吡啶肼基（DPPH）和过氧化氢（H2O2）的清除率分别为95.88 ± 1.22 %和85.35 ± 1.43 %。通过显微和宏观分析评价ccr - p4d对溃疡性结肠炎（UC）大鼠的急性毒性研究和抗溃疡作用。在蛋白质-配体结合相互作用中，Cur对炎症标志物c -反应蛋白、IL-6和TNF-α有明显的抑制作用。最后，ccr - p4d被认为是IBD局部作用治疗药物的替代方案。

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Treatment of ulcerative colitis by sustained release curcumin pellets coated with ethyl ascorbic acid; in-vitro, in-vivo, and in-silico analysis

The present work aimed to protect the rapid reduction of Curcumin (Cur) in alkaline pH by newly synthesized ethyl ascorbic acid (EAA) in dual-coated Cur pellets (CCur-P4d) used for the treatment of inflammatory bowel disease (IBD). Physicochemical and morphological analysis was performed by FTIR, DSC, XRD, and SEM-EDS and in vitro release study. 92.87 ± 6.26 % release of Cur from uncoated Cur pellets (UCur-P4) in 0.1 M HCl at pH 1.2 was controlled to 5 % by coating with Eudragit L-100 (EL-100) which interns increased to 97.99 ± 7.35 % in 100 mM phosphate buffer of pH 7.4. The scavenging activity of 2,2-Diphenyl-1-picrylhydrazyl (DPPH) and hydrogen peroxides (H₂O₂) was 95.88 ± 1.22 % and 85.35 ± 1.43 %, respectively. Acute toxicity study and anti-ulcerative effect of CCur-P4d in ulcerative colitis (UC) induced albino rats were evaluated by microscopic and macroscopic analysis. In protein-ligand binding interaction, Cur showed a potent inhibitory effect on inflammatory markers C-reactive protein, IL-6, and TNF-α. Conclusively, CCur-P4d is considered an alternative to locally acting therapeutics agents used in IBD.

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来源期刊

Colloids and Surfaces B: Biointerfaces 生物-材料科学：生物材料

CiteScore

11.10

自引率

3.40%

发文量

730

审稿时长

42 days

期刊介绍： Colloids and Surfaces B: Biointerfaces is an international journal devoted to fundamental and applied research on colloid and interfacial phenomena in relation to systems of biological origin, having particular relevance to the medical, pharmaceutical, biotechnological, food and cosmetic fields. Submissions that: (1) deal solely with biological phenomena and do not describe the physico-chemical or colloid-chemical background and/or mechanism of the phenomena, and (2) deal solely with colloid/interfacial phenomena and do not have appropriate biological content or relevance, are outside the scope of the journal and will not be considered for publication. The journal publishes regular research papers, reviews, short communications and invited perspective articles, called BioInterface Perspectives. The BioInterface Perspective provide researchers the opportunity to review their own work, as well as provide insight into the work of others that inspired and influenced the author. Regular articles should have a maximum total length of 6,000 words. In addition, a (combined) maximum of 8 normal-sized figures and/or tables is allowed (so for instance 3 tables and 5 figures). For multiple-panel figures each set of two panels equates to one figure. Short communications should not exceed half of the above. It is required to give on the article cover page a short statistical summary of the article listing the total number of words and tables/figures.