Nanoparticles of quercetin-gellan gum conjugate for the controlled delivery of fluconazole

IF 5.4 2区医学 Q1 BIOPHYSICS

Colloids and Surfaces B: Biointerfaces Pub Date : 2025-04-09 DOI:10.1016/j.colsurfb.2025.114681

Azka Fatima , Shahid Shah , Akhtar Rasul , Ghulam Abbas , Abid Mahmood , Muhammad Hanif , Alina Ilyas , Maira Tahir , Safirah Maheen , Iqra Mubeen , Tahira Zaheer , Muhammad Abu Sufian , Saleha Babar , Nader I. Namazi

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引用次数: 0

Abstract

The aim of the study was to formulate the polymeric nanoparticles for the controlled delivery of fluconazole (FCZ) to enhance its absorption, mucosal adherence, and bioavailability. FCZ-loaded gellan gum-quercetin conjugate (GQC) nanoparticles (FCZ-loaded NGQ) were prepared by ionic gelatin method using GQC, surfactants, co-surfactants, and cross-linker. The nanoparticles were then characterized by various physical and chemical analytical tools such as ¹H NMR, FTIR, XRD, DSC, TGA, particle size, zeta potential, and SEM analysis. All the nanoparticles have a nanosized range from 108 nm to 123 nm with a zeta potential of −12 mV to −20 mV which shows their stability. The NGQ3 (optimized formulation) loaded with FCZ have a PDI of 0.20 ± 0.02 and % entrapment efficiency of 32.3 ± 2.8 %. These studies also showed promising results of 85 % drug release, the value for zero order kinetics of R² was 0.999, mucosal adherence of 24 h, and the highest values of permeation was shown by NGQ3 of 60 %. The improved antifungal activity of prepared NGQ3-DL against Candida albicans was observed compared to the pure drug. The nanoparticles may offer enhanced bioavailability and controlled release of FCZ, making them a potential breakthrough in managing FCZ resistant infections.

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槲皮素-结冷胶缀合纳米颗粒用于氟康唑的控制递送

本研究的目的是制备用于氟康唑（FCZ）可控递送的聚合纳米颗粒，以增强其吸收、粘膜粘附性和生物利用度。采用离子明胶法制备了负载结冷胶-槲皮素缀合物（GQC）纳米粒子（负载GQC的NGQ）。采用1H NMR、FTIR、XRD、DSC、TGA、粒度、zeta电位、SEM等理化分析工具对纳米颗粒进行表征。纳米颗粒的粒径范围为108 nm ~ 123 nm， zeta电位为- 12 mV ~ - 20 mV，具有良好的稳定性。优化后的NGQ3的PDI为0.20 ± 0.02，包封率为32.3 ± 2.8%。这些研究也显示出良好的结果，药物释放率为85%，零级动力学R2为0.999，粘膜粘附24 h， NGQ3的渗透率最高，为60%。制备的NGQ3-DL对白色念珠菌的抗真菌活性较纯品有所提高。这些纳米颗粒可能提高FCZ的生物利用度并控制其释放，使其成为控制FCZ耐药感染的潜在突破。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Colloids and Surfaces B: Biointerfaces 生物-材料科学：生物材料

CiteScore

11.10

自引率

3.40%

发文量

730

审稿时长

42 days

期刊介绍： Colloids and Surfaces B: Biointerfaces is an international journal devoted to fundamental and applied research on colloid and interfacial phenomena in relation to systems of biological origin, having particular relevance to the medical, pharmaceutical, biotechnological, food and cosmetic fields. Submissions that: (1) deal solely with biological phenomena and do not describe the physico-chemical or colloid-chemical background and/or mechanism of the phenomena, and (2) deal solely with colloid/interfacial phenomena and do not have appropriate biological content or relevance, are outside the scope of the journal and will not be considered for publication. The journal publishes regular research papers, reviews, short communications and invited perspective articles, called BioInterface Perspectives. The BioInterface Perspective provide researchers the opportunity to review their own work, as well as provide insight into the work of others that inspired and influenced the author. Regular articles should have a maximum total length of 6,000 words. In addition, a (combined) maximum of 8 normal-sized figures and/or tables is allowed (so for instance 3 tables and 5 figures). For multiple-panel figures each set of two panels equates to one figure. Short communications should not exceed half of the above. It is required to give on the article cover page a short statistical summary of the article listing the total number of words and tables/figures.