Colloids and Surfaces B: Biointerfaces最新文献

{"title":"A CRISPR/Cas12a-based colorimetric AuNPs biosensor for naked-eye detection of pathogenic bacteria in clinical samples","authors":"Laibao Zheng ,&nbsp;Chaochuan Zheng ,&nbsp;Weiwei Wang ,&nbsp;Fuyuan Huang ,&nbsp;Yelin Jiang ,&nbsp;Jiahai Lu ,&nbsp;Yongliang Lou","doi":"10.1016/j.colsurfb.2025.114541","DOIUrl":"10.1016/j.colsurfb.2025.114541","url":null,"abstract":"<div><div>Pathogenic bacteria, such as <em>Pseudomonas aeruginosa</em>, pose significant threats to public health due to their multidrug resistance and association with severe infections. Rapid and reliable detection methods are crucial for timely treatment and effective infection control, especially in resource-limited settings. In this study, we developed a CRISPR/Cas12a-based colorimetric biosensor that leverages Cas12a’s trans-cleavage activity to release left single-stranded DNA (lDNA). The released lDNA facilitates hybridization with clDNA-functionalized gold nanoparticles (AuNPs), resulting in a visible color change. The biosensor achieved a detection limit of 10⁰ CFU/reaction for <em>P. aeruginosa</em> within 2 hours, with excellent specificity and robustness, as validated in spiked sputum and blood samples. Clinical testing using 32 blood samples (13 positive, 19 negative) confirmed its high diagnostic accuracy, achieving an AUC of 1 in ROC curve analysis. The platform’s simplicity, robustness, and programmability suggest its broad potential for rapid infectious disease diagnostics, particularly in low-resource settings.</div></div>","PeriodicalId":279,"journal":{"name":"Colloids and Surfaces B: Biointerfaces","volume":"250 ","pages":"Article 114541"},"PeriodicalIF":5.4,"publicationDate":"2025-01-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143078109","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Layered double hydroxides for a synthesis of hierarchical nanostructures: Surface-induced crystallization of antlerite microrods conversed to reusable antimicrobial CuO","authors":"Ilya Shlar ,&nbsp;Yanna Gurianov ,&nbsp;Elena Poverenov","doi":"10.1016/j.colsurfb.2025.114543","DOIUrl":"10.1016/j.colsurfb.2025.114543","url":null,"abstract":"<div><div>A novel strategy for high-yield synthesis of hierarchical metal-oxide nanostructures utilizing layered double hydroxides (LDHs) was developed. LDHs were found to induce surface crystallization of copper hydroxysulfate leading to highly crystalline microrods of antlerite (Cu₃(OH)₄SO₄) via the intermediate stage of brochantite (Cu₄(OH)₆SO₄) in a process that obeys Ostwald's rule of stages. The resultant microrods were converted into hierarchical 3D CuO nanostructures through a straightforward and scalable process. The prepared nanostructures demonstrated total eradication of gram negative (<em>Escherichia coli</em>) and gram positive (<em>Bacillus Cereus</em>) bacteria, with maintained efficacy over reuse cycles. No leaching of antimicrobial material from the nanostructures was obtained, confirming their structural stability and resistance to dissolution. The structures were studied by field emission scanning electron microscopy, X-ray powder diffraction, and Fourier-transform infrared spectroscopy. Two-chamber experiments reinforced the involvement of a surface-induced nucleation mechanism in antlerite formation. The use of LDH for surface-induced nucleation and formation of hierarchical nanostructures, provides innovative cost-effective and simple synthetic approach that eliminates the need for laborious intermediate purification steps. This method can be extended to other metal-based nanostructures, paving the way for new applications in advanced material synthesis.</div></div>","PeriodicalId":279,"journal":{"name":"Colloids and Surfaces B: Biointerfaces","volume":"249 ","pages":"Article 114543"},"PeriodicalIF":5.4,"publicationDate":"2025-01-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143073043","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A fluorinated prodrug strategy enhances the therapeutic index of nanoparticle-delivered hydrophilic drugs","authors":"Liang Liu ,&nbsp;Jun Ge ,&nbsp;Hui Fu ,&nbsp;Jingyu Wang ,&nbsp;Yatong Chen ,&nbsp;Yujiao Sun ,&nbsp;Chengcheng Zhao ,&nbsp;Yunfei Li ,&nbsp;Yingpeng Li","doi":"10.1016/j.colsurfb.2025.114539","DOIUrl":"10.1016/j.colsurfb.2025.114539","url":null,"abstract":"<div><div>The low therapeutic index of hydrophilic drugs with potent pharmacological effects limits their effectiveness and clinical application. A common strategy to enhance hydrophilic drug delivery involves combining amphiphilic prodrugs with nanoparticle delivery systems; however, this approach often fails to overcome exclusion by stem cell-like circulating tumor cells (CTCs). This study introduces a fluorinated prodrug strategy designed to enhance intracellular protein interactions and reduce this exclusion. Danshensu (DAN), known to suppress metastasis by reducing cancer stemness, has limited clinical potential due to its hydrophilicity and resulting low bioavailability. Although aliphatic aromatic amphiphilic prodrug strategies improve hydrophilic drug bioavailability, efficient fluoroalkylation techniques are also useful for synthesizing bioactive fluorine-containing compounds. Inspired by these methods, we propose a novel amphiphilic prodrug approach that attaches aromatic aliphatic chains to enhance DAN’s amphiphilicity and adds fluorinated aromatic rings to strengthen intracellular protein interactions, thereby improving DAN’s intracellular effectiveness. Our studies showed that DAN reduces cancer stemness by inhibiting the β-catenin pathway, and that increasing the lipophilicity of DAN prodrugs enhances their regulatory effect on this pathway, with fluorinated aromatic prodrugs proving more effective than non-fluorinated ones. Additionally, these optimized prodrugs significantly amplified miriplatin’s stemness-suppressing activity. Based on these findings, we designed a hyaluronic acid-based nanoparticle to co-encapsulate both the fluorinated aromatic prodrugs and miriplatin. This formulation exhibited targeted action against CTCs, effectively preventing postoperative metastasis in a breast cancer mouse model and significantly improving survival rates in treated mice. In conclusion, the fluorinated aromatic prodrug strategy offers a promising method for optimizing hydrophilic small-molecule drugs, enhancing their druggability, and preventing postoperative metastasis.</div></div>","PeriodicalId":279,"journal":{"name":"Colloids and Surfaces B: Biointerfaces","volume":"249 ","pages":"Article 114539"},"PeriodicalIF":5.4,"publicationDate":"2025-01-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143078106","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Rigid and soft substrates respectively promote the myocardial differentiation and maturation of human embryonic stem cells using elastic PDMS with thick synthetic coating","authors":"Ling Yang ,&nbsp;Lu Liu ,&nbsp;Pengxia Zhang ,&nbsp;Wen Pan ,&nbsp;Hongxin Huang ,&nbsp;Yongmei Qi ,&nbsp;Yingbin Wang ,&nbsp;Rongzhi Zhang ,&nbsp;Ping Zhou","doi":"10.1016/j.colsurfb.2025.114540","DOIUrl":"10.1016/j.colsurfb.2025.114540","url":null,"abstract":"<div><div>Cardiovascular disease is the predominant cause of mortality and severe disability. Cardiomyocytes (CMs) derived from human embryonic stem cells (hESCs) have good application prospects for treating this disease. Unfortunately, CMs generated via current methods are relatively immature, as proven by defects such as sarcomer-like structures, calcium processing capacity and mitochondrial maturity. Therefore, in this study, tunable PDMS substrates that modified with sufficiently thick synthetic coatings were prepared to regulate both the myocardial differentiation of hESCs and subsequent maturation. Surprisingly, the effect of substrate elasticity on the critical attachment of hESCs and hESC-CMs vanished when common Matrigel coatings were used, but apparent differences were detected in the synthetic group. Rigid substrates promoted the adhesion of hESCs but not hESC-CMs. Moreover, the PDMS substrates with the highest hardness remarkably promoted the myocardial differentiation of hESCs, which was even better than that of the rigid plate group. The softest PDMS achieved the best performance among the groups in terms of the maturation of hESC-CMs, as confirmed by enhanced functional, metabolic, and ultrastructural maturation. This study reveals the real impact of an elastic substrate on the adhesion, differentiation, and maturation of hESC-CMs, which has value for accelerating the development of clinically applicable mature hESC-CMs with high induction efficiency.</div></div>","PeriodicalId":279,"journal":{"name":"Colloids and Surfaces B: Biointerfaces","volume":"250 ","pages":"Article 114540"},"PeriodicalIF":5.4,"publicationDate":"2025-01-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143170085","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Revealing the mechanism of two rimantadine derivatives inhibiting Aβ aggregation and destabilizing Aβ protofibrils by molecular dynamics simulation","authors":"Jiaxing Zhang ,&nbsp;Yufan Yang ,&nbsp;Chengyu Zhang ,&nbsp;Yuefei Wang ,&nbsp;Rongxin Su ,&nbsp;Wei Qi","doi":"10.1016/j.colsurfb.2025.114538","DOIUrl":"10.1016/j.colsurfb.2025.114538","url":null,"abstract":"<div><div>Two rimantadine derivatives, m-aminobenzoyl rimantadine (meta) and p-aminobenzoyl rimantadine (para), have been demonstrated to effectively inhibit the aggregation of amyloid-β (Aβ) peptides. However, the exact atomic-level mechanism remains elusive. In this study, we investigated the inhibitory mechanisms of meta and para on Aβ aggregation. Using replica-exchange and microsecond classical molecular dynamics simulations, we analyzed the conformational ensembles of Aβ dimers and the structure of Aβ(1−40) protofibrils both with and without the rimantadine derivatives. Results showed that meta and para inhibit Aβ aggregation by hydrogen bonds and hydrophobic interactions with Aβ dimers. Meta mainly binds to CHC residues F19 and F20 to disrupt hydrophobic contacts, while para targets β-turns and the K28–V40 region, destabilizing the hydrophobic core and increasing structural flexibility, thus preventing stable dimer formation. Para exhibits a higher binding affinity and is more effective in inhibiting Aβ aggregation and destabilizing protofibrils. These findings provide valuable insights into the atomic-level mechanisms by which meta and para inhibit Aβ aggregation, offering promising avenues for the exploration of potential therapeutics for Alzheimer's disease (AD).</div></div>","PeriodicalId":279,"journal":{"name":"Colloids and Surfaces B: Biointerfaces","volume":"250 ","pages":"Article 114538"},"PeriodicalIF":5.4,"publicationDate":"2025-01-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143170086","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Hollow mesoporous silica nanoparticles for drug formulation and delivery: Opportunities for cancer therapy","authors":"Huiqi Guo,&nbsp;Xia Zhao,&nbsp;Yanping Duan,&nbsp;Jingzhuan Shi","doi":"10.1016/j.colsurfb.2025.114534","DOIUrl":"10.1016/j.colsurfb.2025.114534","url":null,"abstract":"<div><div>The advantages of large surface area, high volume ratio, good biocompatibility, and controllable surface functionalization make hollow mesoporous silica nanoparticles (HMSNs) an ideal drug carrier. HMSNs can achieve high efficiency, targeting, and controlled release by adjusting the microstructure and surface modification of its particles, which makes it broad application prospects in the field of medical therapy, especially in cancer therapy. Numerous studies have shown that preparation method, shape, particle size, hollow inner diameter, aperture and wall thickness of the HMSNs, the characteristics of the drugs, the interaction between the drugs and the carriers, and the external environment all closely affect the drug delivery, release, and efficacy. The external environment includes temperature, pH value, light intensity, magnetic field intensity, enzyme type and concentration, etc. This review summarizes the research progress of HMSNs as carrier materials in the past five years, analyzes the existing problems in the application process and presents the development prospects of HMSNs.</div></div>","PeriodicalId":279,"journal":{"name":"Colloids and Surfaces B: Biointerfaces","volume":"249 ","pages":"Article 114534"},"PeriodicalIF":5.4,"publicationDate":"2025-01-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143057642","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Baicalein-loaded porous silk fibroin microspheres modulate the senescence of nucleus pulposus cells through the NF-κB signaling pathway","authors":"Yanchi Gan ,&nbsp;Jiahui He ,&nbsp;Yan Gong ,&nbsp;Zixian Wu ,&nbsp;De Liang ,&nbsp;Gengyang Shen ,&nbsp;Hui Ren ,&nbsp;Xiaobing Jiang ,&nbsp;Zhaojun Cheng","doi":"10.1016/j.colsurfb.2025.114537","DOIUrl":"10.1016/j.colsurfb.2025.114537","url":null,"abstract":"<div><div>Intervertebral disc degeneration (IVDD), an age-associated degenerative condition, significantly contributes to low back pain, thereby adversely affecting individual health and quality of life, while also imposing a substantial societal burden. Baicalein, a natural flavonoid derived from Scutellaria baicalensis Georgi, demonstrates a range of pharmacological activities, including antioxidant, anti-inflammatory, anti-tumor, and antibacterial properties. This positions it as a promising candidate for the treatment of IVDD through intradiscal drug delivery. However, local degenerative processes and the inherently low fluid exchange within the intervertebral disk are likely to affect drug retention. In this study, we developed baicalein-loaded porous silk fibroin microspheres to extend the drug release profile. Baicalein-loaded porous silk fibroin microspheres were prepared by electrostatic spraying. Subsequent characterization and evaluation of their intrinsic properties were conducted using nuclear magnetic resonance (NMR), scanning electron microscopy (SEM), transmission electron microscopy(TEM), and fourier transform infrared spectroscopy (FTIR). The findings of our study demonstrated that baicalein-loaded porous silk fibroin microspheres exhibited a sustained drug release profile. Consequently, these microspheres effectively inhibited the senescence of nucleus pulposus cells (NPCs), which induced by Tert-butyl hydroperoxide (TBHP). Mechanistic investigation utilizing transcriptome sequencing revealed that the NF-κB signaling pathway is involved in the effects of baicalein-loaded porous silk fibroin microspheres. Furthermore, our findings demonstrated that the microspheres exhibited excellent biocompatibility in rats subcutaneous implantation model. Collectively, we developed a promising biomaterial for the treatment of IVDD, warranting further systematic preclinical investigation.</div></div>","PeriodicalId":279,"journal":{"name":"Colloids and Surfaces B: Biointerfaces","volume":"249 ","pages":"Article 114537"},"PeriodicalIF":5.4,"publicationDate":"2025-01-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143062649","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Development and characterization of potential antibacterial biocomposites: Lysozyme-loaded cellulose-alginate materials","authors":"Carlos Eduardo Camacho-González ,&nbsp;Alejandro Pérez-Larios ,&nbsp;Cesar S. Cardona-Félix ,&nbsp;Sonia G. Sáyago-Ayerdi ,&nbsp;Víctor M. Zamora-Gasga ,&nbsp;Ulises M. López-García ,&nbsp;Jorge A. Sánchez-Burgos","doi":"10.1016/j.colsurfb.2025.114536","DOIUrl":"10.1016/j.colsurfb.2025.114536","url":null,"abstract":"<div><div>The objective of this study was to design and characterize novel biocomposites based on modified cellulose/alginate oligosaccharides loaded with hen egg lysozyme (CLm/AOS/Lyz) as a potential alternative to combat bacterial proliferation. An antimicrobial enzyme, lysozyme, was immobilized within polymeric matrices to enhance its bactericidal capacity and stability. The biocomposites synthesized at pH levels 3, 5, and 8 (CLm/AOS/Lyz₃, CLm/AOS/Lyz₅, and CLm/AOS/Lyz₈, respectively) were analyzed using Fourier transform infrared spectroscopy (FTIR), ultraviolet-visible spectroscopy (UV-Vis), and energy-dispersive X-ray spectroscopy (EDS). Results demonstrated successful lysozyme conjugation to the biocomposite without altering its secondary structure or stability. The biocomposites exhibited irregular morphologies and strong adhesion between CLm/AOS and Lyz, with CLm/AOS/Lyz5 showing the highest nitrogen composition and protein content (2086.43 ± 100.90 µg of bovine serum albumin equivalents). Antibacterial assays revealed significant log reductions in viable <em>E. faecalis</em> cells for CLm/AOS/Lyz₃ and CLm/AOS/Lyz₅ (5.72 ± 0.17 and 5.78 ± 0.24 respectively), concerning the blank (8.04 ± 0.07), even comparable to free lysozyme (5.85 ± 0.35). However, no reduction in viable cell counts was observed for Gram-negative bacteria. This work highlights the potential of lysozyme-loaded cellulose-alginate biocomposites as novel antibacterial agents for effective applications in pharmaceutical and food technology fields.</div></div>","PeriodicalId":279,"journal":{"name":"Colloids and Surfaces B: Biointerfaces","volume":"250 ","pages":"Article 114536"},"PeriodicalIF":5.4,"publicationDate":"2025-01-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143078113","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Borate ester-crosslinked polysaccharide hydrogel reinforced by proanthocyanidins for oral ulcer therapy","authors":"Yan Ge ,&nbsp;Mingjing Wang ,&nbsp;Yang Zhou ,&nbsp;Qiyuan Pan ,&nbsp;Mengna Duan ,&nbsp;Xiao Zhang ,&nbsp;Ruolin Wang ,&nbsp;Yaru Huo ,&nbsp;Yifan Guo ,&nbsp;Wei Shen ,&nbsp;Min Liu","doi":"10.1016/j.colsurfb.2025.114535","DOIUrl":"10.1016/j.colsurfb.2025.114535","url":null,"abstract":"<div><div>Oral ulcers are prone to recurrence and often complicated by bacterial infections. Currently, antibiotics, glucocorticoids, and anesthetics are commonly employed in clinical practice to alleviate symptoms. However, these medications exhibit limited retention in the moist and dynamic environment of the oral cavity, and their long-term use may lead to various side effects or drug resistance. In this study, we designed a borate ester cross-linked oxidation-responsive hydrogel (AHQP hydrogel) for the treatment of oral ulcers. The AHQP hydrogel is composed of phenylboronic acid-functionalized hyaluronic acid, sodium alginate, and quaternized chitosan (QCS), and is further enhanced by the incorporation of proanthocyanidins (PA) to strengthen its borate ester cross-linked network. These natural raw materials comprising the AHQP hydrogel exhibit excellent biocompatibility and degradability, ensuring that the hydrogel is safe for use in the oral cavity. Once the AHQP hydrogel adheres to the wet wound tissue, it degrades in response to reactive oxygen species, thereby releasing QCS and PA to exert therapeutic effects. Our results demonstrated that the AHQP hydrogel exhibits significant antioxidant, anti-inflammatory, antibacterial, and pro-angiogenic properties, thereby facilitating the healing of oral ulcers. Overall, this borate ester cross-linked polysaccharide hydrogel reinforced by PA presents promising prospects for the treatment of oral ulcers.</div></div>","PeriodicalId":279,"journal":{"name":"Colloids and Surfaces B: Biointerfaces","volume":"249 ","pages":"Article 114535"},"PeriodicalIF":5.4,"publicationDate":"2025-01-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143057641","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"One step synthesis of tryptophan-isatin carbon nano dots and bio-applications as multifunctional nanoplatforms","authors":"Kerem Tok ,&nbsp;F. Baris Barlas ,&nbsp;Ece Bayır ,&nbsp;Ahmet Murat Şenışık ,&nbsp;Figen Zihnioglu ,&nbsp;Suna Timur","doi":"10.1016/j.colsurfb.2025.114533","DOIUrl":"10.1016/j.colsurfb.2025.114533","url":null,"abstract":"<div><div>The development of natural molecule-derived carbon nano dots (CNDs) marks a significant advancement in biocompatible and sustainable nanomaterials. Tryptophan, capable of crossing the blood-brain barrier (BBB), serves as a precursor to numerous pharmacologically active compounds, while isatin and its derivatives have demonstrated anti-tumor effects, including against brain cancers. This study aimed to synthesize fluorescent CNDs from tryptophan-isatin hybrid precursor and explore their applications in glioblastoma treatment. These CNDs were characterized using techniques such as TEM, SEM-EDS, FTIR, XPS, Raman spectroscopy and UV-Vis spectrophotometry. In vitro tests using the U-87 glioblastoma cell line evaluated cell viability, affinity, and BBB permeability. The CNDs, between 4 and 7 nm in size, exhibited blue and green fluorescence, with no cytotoxic effects observed at concentrations up to 25 µg/mL. The highest BBB permeability rate was determined as 4.3 × 10⁻⁵ cm/s. Additionally, the CNDs demonstrated radiotherapeutic properties, leading to a 51 % reduction in cell viability. This research contributes to nanomedicine by introducing a novel biocompatible material with potential for targeted brain cancer imaging and therapy, while also suggesting broader applications beyond glioblastoma.</div></div>","PeriodicalId":279,"journal":{"name":"Colloids and Surfaces B: Biointerfaces","volume":"249 ","pages":"Article 114533"},"PeriodicalIF":5.4,"publicationDate":"2025-01-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143035452","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}