Colloids and Surfaces B: Biointerfaces最新文献

{"title":"Microarc oxidation-PBAT composite coating on EK30 biodegradable magnesium alloys to enhance corrosion resistance and cytocompatibility","authors":"Weisheng Cao ,&nbsp;Siyu Zhu ,&nbsp;Weiqiang Wang ,&nbsp;Yahui Wang ,&nbsp;Shuaikang Yang ,&nbsp;Haiyang Sun","doi":"10.1016/j.colsurfb.2025.114720","DOIUrl":"10.1016/j.colsurfb.2025.114720","url":null,"abstract":"<div><div>The rapid degradation rate of biodegradable magnesium alloys restricts their use in medical implants. Therefore, designing a protective coating with a slow degradation rate and good biocompatibility is crucial. For biodegradable magnesium alloy stents, it is equally important to enhance the material's corrosion resistance and ensure the coating's deformation adaptability to the stent. This study, utilized a combination of micro-arc oxidation (MAO) and dip-coating techniques to develop a novel composite coating comprising an MAO base layer and a poly(butylene adipate-co-terephthalate) (PBAT) outer layer on EK30 magnesium alloy. This composite coating was designed to enhance the corrosion resistance and biocompatibility of EK30 magnesium alloy for stent applications. The surface characteristics, corrosion resistance, <em>in vitro</em> cytocompatibility, and deformation adaptability of the composite coating to the stent were evaluated. The MAO-PBAT composite coating demonstrated a low corrosion current density (<em>I</em>_corr = 2.381 ×10⁻⁸ A/cm²), three orders of magnitude lower than that of unmodified EK30 magnesium alloy. Live/dead cell staining results confirmed that the composite coating exhibited good cytocompatibility with human aortic endothelial cells (HAECs) and human aortic smooth muscle cells (HASMCs). Observations of the stent treated with the composite coating during crimping and expansion showed that the composite coating possessed excellent deformation adaptability. These results indicate that the MAO-PBAT composite coating has significant potential for vascular stent application.</div></div>","PeriodicalId":279,"journal":{"name":"Colloids and Surfaces B: Biointerfaces","volume":"253 ","pages":"Article 114720"},"PeriodicalIF":5.4,"publicationDate":"2025-04-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143851930","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Quaternized chitosan-based injectable self-healing hydrogel for improving wound management in aging populations","authors":"Guochang Chen ,&nbsp;Xiaowei Xun ,&nbsp;Haiyong Ao ,&nbsp;Ziqing Chen ,&nbsp;Dingyun Wang ,&nbsp;Maohu Wang ,&nbsp;Dongxue Zhang ,&nbsp;Minzhuo Liu ,&nbsp;Guanghua Guo","doi":"10.1016/j.colsurfb.2025.114721","DOIUrl":"10.1016/j.colsurfb.2025.114721","url":null,"abstract":"<div><div>The clinical management and treatment of skin wounds in the elderly present significant challenges due to changes in skin structure and function. This study introduces a novel injectable self-healing hydrogel composed of quaternized chitosan and carboxymethyl chitosan (HACC/CMCS, HC), designed through electrostatic interactions. Its excellent injectability and self-healing properties enhance the application of hydrogel dressings and prolong their functional lifespan. Moreover, the adhesion and flexibility of HC hydrogel contribute to their stability in highly dynamic regions, thereby preventing detachment and enhancing their hemostatic function. The material exhibits excellent biocompatibility and possesses antibacterial properties that protect wounds from external microbial damage, thereby reducing the risk of infection while maintaining a moist environment that facilitates healing. Importantly, the <em>in vivo</em> test have demonstrated that the HC hydrogel significantly enhances collagen deposition, reduces senescent cell accumulation, and accelerates wound closure. Therefore, this study offers a safe, effective, and cost-efficient solution for managing wounds in the aging population.</div></div>","PeriodicalId":279,"journal":{"name":"Colloids and Surfaces B: Biointerfaces","volume":"253 ","pages":"Article 114721"},"PeriodicalIF":5.4,"publicationDate":"2025-04-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143859858","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Engineered bacterial outer membrane vesicles co-delivering Angio-3 and doxorubicin to enhance tumor therapy","authors":"Shuai Zhao ,&nbsp;Wenxuan Xu ,&nbsp;Wanting Zeng ,&nbsp;Shuailin He ,&nbsp;Xuan Luo ,&nbsp;Dejie Ge ,&nbsp;Yamin Duan ,&nbsp;Rui Han ,&nbsp;Chin-Yu Chen ,&nbsp;Yong Yang ,&nbsp;Yunhong Hu ,&nbsp;Cheng Zhang","doi":"10.1016/j.colsurfb.2025.114707","DOIUrl":"10.1016/j.colsurfb.2025.114707","url":null,"abstract":"<div><div>Bacterial outer membrane vesicles (OMVs) have emerged as versatile nanomaterial-based drug delivery systems that can stimulate systemic immune responses and facilitate precise co-delivery of multiple therapeutic agents. This study introduces a bioengineering approach that enables the co-delivery of the angiogenesis inhibitor Angio-3 and the chemotherapeutic agent doxorubicin (DOX) within OMVs, creating a potent antitumor therapeutic platform. Angio-3 displayed on the surface of OMVs inhibited angiogenesis and decreased vascular permeability, which in turn impeded the supply of nutrients necessary for tumor growth. Moreover, intrinsic properties of OMVs triggered a systemic immune response. Both <em>in vitro</em> and <em>in vivo</em> studies, including a CT26 tumor-bearing mouse model, have demonstrated that the OMV@A&amp;D-based therapeutic regimen, which integrates antiangiogenesis, chemotherapy, and immune activation, significantly suppresses tumor proliferation. This study highlights the potential of bioengineered OMVs in revolutionizing cancer therapy by offering a multifaceted and synergistic platform that enhances therapeutic outcomes.</div></div>","PeriodicalId":279,"journal":{"name":"Colloids and Surfaces B: Biointerfaces","volume":"253 ","pages":"Article 114707"},"PeriodicalIF":5.4,"publicationDate":"2025-04-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143851928","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Marine polysaccharides hydrogel with encapsulated mesalazine for the treatment of ulcerative colitis: Integrative effects on inflammation, microbiota, and mucosal repair","authors":"Teng Xiao ,&nbsp;Zhenxin Yu ,&nbsp;Haomin Yang ,&nbsp;Jun You ,&nbsp;Xiaochen Wu","doi":"10.1016/j.colsurfb.2025.114722","DOIUrl":"10.1016/j.colsurfb.2025.114722","url":null,"abstract":"<div><div>Ulcerative colitis is a chronic non-specific inflammatory disease of the intestine that significantly impacts patient quality of life. This study introduces a OF/CC/SM hydrogel containing oxidized fucoidan (OF), carboxymethyl chitosan (CC), and silk sericin-stabilized mesalazine (SM), designed for rectal administration to target mesalazine delivery specifically to the colon. The OF/CC/SM hydrogel demonstrated good biocompatibility (cell compatibility &gt; 99 %), injectability, and adhesion strength, ensuring effective mesalazine retention and release. <em>In vitro</em> assays confirmed the hydrogel's antioxidant and anti-inflammatory properties, which were further validated <em>in vivo</em> using a mouse model of ulcerative colitis. Rectal administration of OF/CC/SM hydrogel significantly relieved weight loss, lowered disease activity index scores, and prevented intestinal shortening associated with dextran sulfate sodium (DSS) treatment. The hydrogel decreased the expression of proinflammatory cytokines (e.g., tumor necrosis factor-α (TNF-α) and interleukin-1β (IL-1β)), while normalized the level of biomarkers (e.g., inducible nitric oxide synthase (iNOS), myeloperoxidase (MPO), catalase (CAT), and malondialdehyde (MDA)). Additionally, the OF/CC/SM hydrogel modulated the gut microbiota, increasing beneficial bacteria while decreasing potentially harmful species. Histopathological analysis revealed a reduction in inflammatory infiltration and improved mucosal architecture. Additionally, <em>in vivo</em> imaging studies confirmed sustained presence of OF/CC/SM hydrogel in the intestines following rectal administration, highlighting its potential for enhanced therapeutic efficacy in treating ulcerative colitis.</div></div>","PeriodicalId":279,"journal":{"name":"Colloids and Surfaces B: Biointerfaces","volume":"253 ","pages":"Article 114722"},"PeriodicalIF":5.4,"publicationDate":"2025-04-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143851929","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effects of phenolic hydroxyl group structures of Alaska pollock gelatin-based adhesives on soft tissue adhesion and biocompatibility","authors":"Mari Miyata ,&nbsp;Hiyori Komatsu ,&nbsp;Shima Ito ,&nbsp;Shiharu Watanabe ,&nbsp;Satsuki Minamisakamoto ,&nbsp;Tetsushi Taguchi","doi":"10.1016/j.colsurfb.2025.114715","DOIUrl":"10.1016/j.colsurfb.2025.114715","url":null,"abstract":"<div><div>Marine mussels adhere to various types of substrates in aqueous/wet environments, and their adhesion is controlled by the catechol structure of the phenolic hydroxyl groups. Tissue adhesives with a catechol structure have been designed based on this biomimetic adhesion mechanism. However, the effects of the chemical structure of the phenolic hydroxyl groups on tissue adhesion and biocompatibility have not yet been systematically investigated. In this study, various phenolic hydroxyl group-modified Alaska pollock gelatins (nHy-ApGltns (n = 0, 1, 2, 3)) were synthesized and their function as tissue adhesives with a poly(ethylene glycol)-based 4-armed crosslinker (4S-PEG) was evaluated. The nHy-ApGltn adhesives showed increases in bulk strength and decreases in swelling compared to the original-ApGltn (Org-ApGltn) adhesive. The tensile stress and Young's modulus of the nHy-ApGltn adhesives increased with the introduction of nHy groups. The burst strength of the nHy-ApGltn adhesives had a maximum value at each content; however, a high content of nHy in the nHy-ApGltn adhesives had a negative effect on the burst strength. The burst strength of the porcine aorta with all the nHy-ApGltn adhesives was higher than that with human blood pressure. From histological observations after burst strength measurements, the 2Hy- and 3Hy-ApGltn adhesives showed stable interfacial adhesion compared to the Org-, 0Hy-, and 1Hy-ApGltn adhesives. The nHy-ApGltn (n = 0, 3) adhesives implanted subcutaneously in rats completely degraded within 64 days without severe inflammation or foreign body reactions. Therefore, nHy-ApGltn adhesives with low nHy content have excellent tissue adhesion and biocompatibility in wet environments and have the potential for use in biomedical applications.</div></div>","PeriodicalId":279,"journal":{"name":"Colloids and Surfaces B: Biointerfaces","volume":"253 ","pages":"Article 114715"},"PeriodicalIF":5.4,"publicationDate":"2025-04-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143855697","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Reduced graphene oxide quantum dots/manganese dioxide/glucose oxidase nanoparticles for cascade catalytic cancer treatment in multimodal starvation therapy-augmented chemodynamic/photothermal therapy","authors":"Thejas P. Premji ,&nbsp;Banendu Sunder Dash ,&nbsp;Yu-Jen Lu ,&nbsp;Darshan Tagadur Govindaraju ,&nbsp;Jyh-Ping Chen","doi":"10.1016/j.colsurfb.2025.114713","DOIUrl":"10.1016/j.colsurfb.2025.114713","url":null,"abstract":"<div><div>Combination cancer therapy can boost the overall treatment efficacy using functional nanomaterials that specifically target cancer cells. Furthermore, the treatment outcome can be improved by focusing on specific characteristics in the tumor microenvironment (TME). In this study, tumor-targeting multifunctional nanoparticles were constructed from reduced graphene oxide quantum dots (rGOQD), manganese dioxide (MnO₂), glucose oxidase (GOx), and cell-penetrating peptide (CPP). The rGOQD/MnO₂/GOx/CPP nanoparticles can treat tumors by strengthening chemodynamic/photothermal therapy (CDT/PTT) with starvation therapy (ST). The MnO₂ reacts with high concentrations of endogenous H₂O₂ in an acidic TME to produce reactive oxygen species (ROSs) from Mn²⁺. The highly cytotoxic hydroxyl radical (•OH) kills cancer cells and initiates CDT. The MnO₂ can also consume the •OH scavenger glutathione (GSH) in cancer cells and eliminate their antioxidant defense. The GOx oxidizes glucose to cause cancer cell glucose starvation for ST, which produces H₂O₂ to boost the efficacy of CDT. By consuming glucose, ST mediated by GOx leads to reduced ATP production in the glycolysis pathway. This downregulates the expression of ATP-dependent heat shock proteins that provide cancer cell thermal resistance when the photothermal agent rGOQD is irradiated with near-infrared (NIR) light for PTT. Therefore, we prepare different rGOQD-based nanoparticles and characterize their physicochemical and biological properties. The nanoparticles were studied in vitro against U87 glioblastoma cells for targeted cancer therapy. Using nude mice bearing subcutaneous U87 tumors, the in vivo study indicates rGOQD/MnO₂/GOx/CPP plus NIR irradiation can substantially inhibit the tumor growth rate without causing adverse effects from CPP-mediated trimodal ST/CDT/PTT.</div></div>","PeriodicalId":279,"journal":{"name":"Colloids and Surfaces B: Biointerfaces","volume":"253 ","pages":"Article 114713"},"PeriodicalIF":5.4,"publicationDate":"2025-04-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143863673","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Mannose modified graphene oxide drug-delivery system targets cancer stem cells and tumor-associated macrophages to promote immunotherapeutic efficacy","authors":"Jiapu Wang ,&nbsp;Ziwei Liang ,&nbsp;Yuhui Wang ,&nbsp;Qi Liu ,&nbsp;Shaojie Wang ,&nbsp;Jie Wang ,&nbsp;Ruxin Duan ,&nbsp;Liqin Zhao ,&nbsp;Yan Wei ,&nbsp;Di Huang","doi":"10.1016/j.colsurfb.2025.114710","DOIUrl":"10.1016/j.colsurfb.2025.114710","url":null,"abstract":"<div><div>Hepatocellular carcinoma (HCC) ranks among the most lethal tumors globally, characterized by high propensity for recurrence and metastasis. Consequently, the imperative challenge is to pioneer novel pharmaceuticals and therapeutic methodologies for efficacious HCC treatment. In the present study, we successfully synthesized a mannose, polyethylene glycol, and polyethyleneimine modified graphene oxide based LDN193189-delivery system (PGPML) for remodeling the HCC tumor microenvironment (TME) though targeting cancer stem cells. These PGPML nanoparticles possess the capability to target cancer stem cell and M2 tumor-associated macrophages (TAMs) through specific binding to different mannose receptors. Within the acidic tumor microenvironment, the PGPML nanoparticles could inhibit proliferation, migration and reverse the epithelial-mesenchymal transition, which efficiently displayed the anti-tumor efficacy with HCC tumors <em>in vitro</em> and <em>in vivo</em>. Mechanically, the PGPML nanoparticles significantly downregulated the expression of cancer stem cell marker CD133, attenuates PD-L1 expression within TME, thereby reducing tumor stemness characteristics, promoting T cell activation and macrophage M2-M1 repolarization to remodel TME, augments the levels of reactive oxygen species (ROS) and tumor necrosis factor-alpha (TNF-α) in tumor cells, fostering tumor cell demise. Collectively, our findings underscore that a synergistic treatment approach combining cancer stem cell-targeted therapy with immunotherapy holds promise as a potential therapeutic strategy for HCC.</div></div>","PeriodicalId":279,"journal":{"name":"Colloids and Surfaces B: Biointerfaces","volume":"253 ","pages":"Article 114710"},"PeriodicalIF":5.4,"publicationDate":"2025-04-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143869511","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Dual-action MOF-on-MOF hydrogel: A chemo-photodynamic strategy for enhanced antibacterial activity and infected wound healing","authors":"Yuze Dong ,&nbsp;Jiahang Si ,&nbsp;Jie Feng,&nbsp;Tiantian Xu,&nbsp;Xinyu Xiao,&nbsp;Yalan Ji,&nbsp;Ruikang Zhang,&nbsp;Xiaohan Sun,&nbsp;Yingnan Zhu","doi":"10.1016/j.colsurfb.2025.114712","DOIUrl":"10.1016/j.colsurfb.2025.114712","url":null,"abstract":"<div><div>Open skin wounds are susceptible to bacterial infections, which can delay healing and even trigger life-threatening complications. The improper and prolonged use of antibiotics can accelerate bacterial resistance, complicating the treatment of clinical infections. Therefore, there is an urgent need for effective antibiotic-free therapeutic strategies to treat bacterial infections in wounds. In this study, we loaded the growth factor Dimethyloxalylglycine (DMOG) into the pores of PCN-224 and subsequently deposited 2-Methylimidazole zinc salt (ZIF-8) on its surface, creating an injectable hydrogel based on a MOF-on-MOF design. This approach leverages metal ion release in conjunction with photodynamic therapy (PDT) to achieve effective antibacterial activity. Additionally, the injectable hydrogel can adapt to various wound morphologies and enable hemostasis for acute tissue injuries due to its fast gelation speed and adhesiveness. Meanwhile, the sustained release of DMOG promotes angiogenesis. Results demonstrated that the GelMA/HA/DMOG@PCN-224/ZIF-8 (GelMA/HA/D@PZ) hydrogel achieves a 99.9 % bactericidal rate against Staphylococcus aureus, Escherichia coli, and MRSA, while also significantly promoting the healing of infected wounds. Therefore, the GelMA/HA/D@PZ composite material facilitates initial hemostasis, mid-term antibacterial activity, and long-term angiogenesis, providing a novel, efficient, and safe approach to promote the healing of infected wounds.</div></div>","PeriodicalId":279,"journal":{"name":"Colloids and Surfaces B: Biointerfaces","volume":"253 ","pages":"Article 114712"},"PeriodicalIF":5.4,"publicationDate":"2025-04-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143860510","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Sulfonated covalent organic frameworks (COF)/polyethersulfone (PES) membrane with enhanced hemocompatibility for blood oxygenation","authors":"Yue Huang ,&nbsp;Lunhao Zhi ,&nbsp;Chong Cheng ,&nbsp;Qiang Wei ,&nbsp;Shudong Sun ,&nbsp;Changsheng Zhao","doi":"10.1016/j.colsurfb.2025.114716","DOIUrl":"10.1016/j.colsurfb.2025.114716","url":null,"abstract":"<div><div>In extracorporeal membrane oxygenation (ECMO) treatment, designing membrane with self-anticoagulant properties can solve problems resulting from the adverse effect of anticoagulants. In this study, 2,4,6-Triformylphloroglucinol (Tp) and 2,5-Diaminobenzenesulfonic acid (Pa-SO₃H) were applied to grow a sulfonated COF film in situ on the polyethersulfone (PES) membrane. The introduction of sulfonic groups increased the hydrophilicity and electronegativity of the TpPa COF film, improved its anti-protein adhesion properties, maintained the normal morphology of blood cells, and endowed the COF film with antithrombotic properties. In the porcine blood circulation test, the duration to increase So₂ (O₂ saturation) from ∼75–95 % in TpPa-SO₃H COF/PES membrane (M-TpPa-SO₃H) was 70 min shorter than that in TpPa COF/PES membrane (M-TpPa). This preparation method is applicable to the preparation of not only flat membranes, but also hollow fiber membranes. These findings emphasize the potential of M-TpPa-SO₃H in ECMO applications, providing superior antithrombotic property and CO₂ efflux potential.</div></div>","PeriodicalId":279,"journal":{"name":"Colloids and Surfaces B: Biointerfaces","volume":"253 ","pages":"Article 114716"},"PeriodicalIF":5.4,"publicationDate":"2025-04-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143844460","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"3D-printed scaffolds: Incorporating dexamethasone microspheres and BMP2 for enhanced osteogenic differentiation of human mesenchymal stem cells","authors":"Jung Hyun Noh ,&nbsp;Yejin Lee ,&nbsp;Moon Suk Kim","doi":"10.1016/j.colsurfb.2025.114705","DOIUrl":"10.1016/j.colsurfb.2025.114705","url":null,"abstract":"<div><div>This study investigates the fabrication and evaluation of 3D-printed scaffolds (G-scaffolds) incorporating dexamethasone-loaded microspheres (Dex-M) and bone morphogenetic protein 2 (BMP2) to enhance osteogenic differentiation of human mesenchymal stem cells (hMSCs). Dex-M was prepared using an ultrasonic atomizer, achieving a high encapsulation efficiency and uniform particle size. The G-scaffolds were precisely printed using photoactive bioprinting, creating Dex-M+BMP2 +G-scaffolds. In vitro release studies demonstrated sustained Dex release over 6 weeks, with the Dex-M+BMP2 +G-scaffold significantly reducing the initial burst release and maintaining stable levels of osteogenic factors. Cytotoxicity assays confirmed the biocompatibility of the scaffolds, showing no adverse effects on hMSC viability. Osteogenic differentiation was assessed via RT-PCR, revealing that the Dex-M+BMP2 +G-scaffold exhibited the highest expression levels of critical osteogenic markers (ON, OP, OC, and COL1A) compared with the other scaffold formulations. Calcium deposition and elemental analysis also demonstrated enhanced mineralization in the Dex-M+BMP2 +G-scaffold group, with calcium and phosphate levels 3.9–1.7 times higher than in the other groups. Overall, the Dex-M+BMP2 +G-scaffold effectively promoted osteogenic differentiation and mineralization of hMSCs, underscoring its potential as a promising biomaterial for bone tissue engineering applications.</div></div>","PeriodicalId":279,"journal":{"name":"Colloids and Surfaces B: Biointerfaces","volume":"253 ","pages":"Article 114705"},"PeriodicalIF":5.4,"publicationDate":"2025-04-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143855699","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}