Colloids and Surfaces B: Biointerfaces最新文献

{"title":"Time-dependent corrosion behavior of EH36 steel caused by Pseudomonas aeruginosa based on big data monitoring technology.","authors":"Shihang Lu, Nianting Xue, Mingxu Gao, Shiqiang Chen, Renzheng Zhu, Xinyu Wang, Guangzhou Liu, Wenwen Dou","doi":"10.1016/j.colsurfb.2024.114349","DOIUrl":"10.1016/j.colsurfb.2024.114349","url":null,"abstract":"<p><p>Marine microbial corrosion poses a significant threat to the safe service of marine engineering equipment. Previous studies have often failed to thoroughly analyze the continuous and prolonged microbial corrosion process, resulting in an incomplete understanding of microbial corrosion mechanisms involved at various stages and the development of ineffective control strategies. This study employed a corrosion big data online real-time monitoring technique to investigate the time-dependent corrosion behavior of EH36 steel caused by Pseudomonas aeruginosa in aerobic environments over a 30-d incubation period. It was found that P. aeruginosa accelerated the corrosion of EH36 steel in the early stages by enhancing the cathodic oxygen reduction process. As oxygen levels declined, P. aeruginosa transitioned from aerobic to anaerobic respiration, promoting corrosion through biocatalytic nitrate reduction. In the later stages, the reduction in sessile cell counts, extreme low oxygen concentration, and dense surface film increased the charge transfer and film resistances, ultimately leading to corrosion inhibition. The weight loss and electrochemical data confirmed the effectiveness of the big data monitoring technique in investigating microbial corrosion, which provides new approaches for diagnosing and preventing microbial corrosion.</p>","PeriodicalId":279,"journal":{"name":"Colloids and Surfaces B: Biointerfaces","volume":"245 ","pages":"114349"},"PeriodicalIF":5.4,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142602386","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Imparting insoluble-soluble property to Cyt c by immobilizing Cyt c in UCST-pH dual responsive polymer for highly sensitive detection of phenol.","authors":"Yuanyuan Wang, Weimin Guan, Yulin Yang, Huiling Lan, Yu Wang, Yun Wang, Juan Han, Lei Wang","doi":"10.1016/j.colsurfb.2024.114344","DOIUrl":"10.1016/j.colsurfb.2024.114344","url":null,"abstract":"<p><p>Immobilization of enzymes in porous organic framework (POF) materials is popular strategy to stabilize enzymes. For such solid enzyme catalysis system, improving the catalytic efficiency is challenging due to the diffusion resistance from solid-liquid interface and inner pores. Here, UCST-pH dual responsive polymeric carrier (PEG-b-PAAm-b-P(GMA-co-AAc)) was synthesized to immobilize cytochrome c (Cyt c), which impart the reversibly insoluble-soluble property to the immobilized Cyt c. The PEG-b-PAAm-b-P(GMA-co-AAc) could serve as an insoluble-soluble matrix to fast and efficiently immobilize Cyt c via covalent attachment, achieving a remarkable 92 % loading efficiency within just 120 min. The obtained insoluble PEG-b-PAAm-b-P(GMA-co-AAc)-Cyt c micelles exhibited an improvement in thermal, pH stability and reusability. The completely soluble PEG-b-PAAm-b-P(GMA-co-AAc)-Cyt c conjugates accelerated substrate diffusion and then enhanced the catalytic efficiency. These excellent advantages led to low detection limit (1.99 μM), lower than the presently reported biosensors based on enzyme mimics in the colorimetric detection of phenol. This UCST-pH dual responsive window presents a new platform to efficiently control the immobilization and release of enzymes, which will achieve excellent stability and catalytic efficiency.</p>","PeriodicalId":279,"journal":{"name":"Colloids and Surfaces B: Biointerfaces","volume":"245 ","pages":"114344"},"PeriodicalIF":5.4,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142610803","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Colon-targeted self-assembled nanoparticles loaded with berberine double salt ameliorate ulcerative colitis by improving intestinal mucosal barrier and gut microbiota.","authors":"Yalong Wang, Yan Chen, Hongjuan Zhang, Shihui Yu, Gang Yuan, Haiyan Hu","doi":"10.1016/j.colsurfb.2024.114353","DOIUrl":"10.1016/j.colsurfb.2024.114353","url":null,"abstract":"<p><p>Ulcerative colitis (UC) is a chronic, recurrent inflammatory bowel disease marked by disturbances in intestinal mucosal barriers, persistent inflammation, oxidative stress, and dysbiosis of the intestinal microbiota. Traditional treatments often fail to adequately address these issues, primarily targeting inflammation. To address these limitations, this study developed an innovative approach using self-assembled nanoparticles for oral administration that target colonic inflammation. Berberine hydrochloride and ursodeoxycholic acid were combined to form a double salt (BeU), enhancing solubility and encapsulation. An amphiphilic polymer (FU-PA) was created by esterifying fucoidan with palmitic acid. FU-PA/BeU nanoparticles were prepared using the nanoprecipitation method and further encapsulated in acid-resistant sodium alginate microspheres (FU-PA/BeU NPs@MS) for targeted delivery to colonic lesions. The aggregation rate of nanoparticles with mucus was significantly reduced to 59 % of free berberine, while the apparent permeability coefficient increased by 2.4 times. In vitro, FU-PA/BeU NPs effectively targeted inflammatory macrophages, reducing IL-6 and NO levels while increasing IL-10 level (to 42.5 %, 26.8 %, and 539 % of the LPS-treated group, respectively). Additionally, the ABTS and DPPH radical scavenging capabilities of FU-PA/BeU NPs were 177.8 % and 151.7 % of BeU, respectively. In dextran sulphate sodium-induced UC mice, oral FU-PA/BeU NPs@MS significantly improved epithelial and mucosal barriers, restored gut microbiota diversity, reduced inflammation and oxidative stress. Remarkably, the mean colon length in the FU-PA/BeU NPs@MS group was 1.2 times longer than that in the sulfasalazine group. These dual-targeted FU-PA/BeU NPs@MS show great potential for UC treatment.</p>","PeriodicalId":279,"journal":{"name":"Colloids and Surfaces B: Biointerfaces","volume":"245 ","pages":"114353"},"PeriodicalIF":5.4,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142602365","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Enhanced therapeutic intervention of curcumin loaded in exosomes derived from milk in alleviating retinal pigment epithelial cells damage.","authors":"Shida Wu, Kuiyou Wang, Qiyan Lv, Mingqian Tan","doi":"10.1016/j.colsurfb.2024.114325","DOIUrl":"10.1016/j.colsurfb.2024.114325","url":null,"abstract":"<p><p>The macula, a small but highly important area in the retina, is crucial for healthy vision. Age-related macular degeneration is responsible for approximately 8.7 % of blindness worldwide, and affected individuals are burgeoning. The age-related macular degeneration is often triggered by oxidative stress and excessive inflammation that damage the retinal pigment epithelial cells in the macula. Curcumin, a potent antioxidant and anti-inflammatory carotenoid, is hampered by low compatibility and stability issues in food science. Innovatively, this study harnessed milk-derived exosomes as a novel delivery method yielding a curcumin-infused system (curcumin@exosome) to increase its biocompatibility and stability. This fusion not only curbed excessive reactive oxygen species but also neutralized H₂O₂-induced mitochondrial disruption in cellular models. It revitalized retinal pigment epithelial cells, reverting their function near to baseline in vitro. The curcumin@exosome outshined in subduing pro-inflammatory cytokines tumor necrosis factor-α and interleukin-1β induced by sodium iodate. This study illuminates that the curcumin@exosome is promise as a therapeutic intervention for retinal ailments marked by oxidative and inflammatory distress.</p>","PeriodicalId":279,"journal":{"name":"Colloids and Surfaces B: Biointerfaces","volume":"245 ","pages":"114325"},"PeriodicalIF":5.4,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142454506","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Super hydrophilic and super oleophobic carbon nanotube/TiO₂ composite membranes for efficient separation of algal-derived oil/water emulsions.","authors":"Ali Ahmadi, Soheila Shokrollahzadeh, Abdolreza Samimi, Alireza Ashori","doi":"10.1016/j.colsurfb.2024.114491","DOIUrl":"https://doi.org/10.1016/j.colsurfb.2024.114491","url":null,"abstract":"<p><p>The separation of oil from microalgae aqueous emulsions is a critical step in producing algal-derived biofuels and nutraceuticals. This study presents the development of super hydrophilic and super oleophobic composite membranes to efficiently separate algal oil from oil/water emulsions. Carbon nanotubes (CNTs) were functionalized with polydopamine (PDA), polyethylene glycol (PEG), and titanium dioxide (TiO₂) nanoparticles and coated onto a mixed cellulose ester (MCE) substrate to fabricate the composite membranes. Two distinct incorporation methods were employed for TiO_2; direct nanoparticle incorporation and surface coating onto the CNT/PDA network. The membranes were comprehensively characterized using FTIR, SEM, EDS, contact angle measurements, and AFM analysis. The synthesized MCE@CNT/PDA/NP-TiO₂ membrane exhibited super hydrophilicity with a water contact angle of 6.3° and underwater super oleophobicity with oil contact angles up to 172°. Membrane performance evaluation using a Nannochloropsis salina microalgae oil/water emulsion revealed excellent flux up to 9238 L m^-2 h^-1 bar^-1 and oil rejection as high as 98.6 % for the TiO₂-incorporated membranes. Additionally, these membranes demonstrated superior antifouling properties, maintaining over 90 % of initial flux even after five separation cycles. Incorporating TiO₂ nanoparticles significantly enhanced the membrane's hydrophilicity, oleophobicity, antifouling capability, and stability under extreme pH conditions. The developed composite membranes show great potential for efficient and cost-effective separation of algal oil from microalgae cultivation systems.</p>","PeriodicalId":279,"journal":{"name":"Colloids and Surfaces B: Biointerfaces","volume":"248 ","pages":"114491"},"PeriodicalIF":5.4,"publicationDate":"2024-12-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142930001","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Robust visualization of membrane protein by aptamer mediated proximity ligation assay and Förster resonance energy transfer.","authors":"Ying Li, Meiqi Qian, Yuping Cheng, Xue Qiu","doi":"10.1016/j.colsurfb.2024.114486","DOIUrl":"https://doi.org/10.1016/j.colsurfb.2024.114486","url":null,"abstract":"<p><p>In situ cell imaging plays a crucial role in studying physiological and pathological processes of cells. Proximity ligation assay (PLA) and rolling circle amplification (RCA) are commonly used to study the abundance and interactions of biological macromolecules. The most frequently applied strategy to visualize the RCA products is with single-fluorophore probe, however, cellular auto-fluorescence and unbound fluorescent probes could interfere with RCA products, leading to non-specific signals. Here, we present a novel approach combining aptamer mediated PLA, RCA, and Förster Resonance Energy Transfer (FRET), namely Apt-PLA-RCA-FRET, for sensitive in situ imaging and analysis of the abundances and interactions of membrane proteins such as tetraspanin CD63 and human epidermal growth factor receptor 2 (HER2). Apt-RCA-FRET was initially designed to show its ability to assess the abundance of target proteins on different cells. Dual functional oligonucleotides served as both the aptamer for recognizing specific membrane proteins and the primer of circular DNA for following RCA process, and the resulting RCA products were subsequently imaged by FRET signals from Cy3 to Cy5 probes which hybridized sequentially on them. FRET was demonstrated to show its great potential to resist the interferences of nonspecific fluorescence compared to single-fluorophore strategies. PLA was then introduced to Apt-RCA-FRET to investigate the spatial localization of different proteins on cell membrane and their interactions. Our approach utilizing aptamer as membrane proteins recognition element simply converted the abundance of proteins into nucleic acid signals and facilitated the following signal amplification, thus it serves as an important alternative to methods typically based on antibody and presents a more robust and sensitive method for analyzing the abundances of different cell membrane proteins and their spatial localization, which offers valuable insights into physiological and pathological processes of cells.</p>","PeriodicalId":279,"journal":{"name":"Colloids and Surfaces B: Biointerfaces","volume":"248 ","pages":"114486"},"PeriodicalIF":5.4,"publicationDate":"2024-12-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142929995","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Thioether functionalized degradable poly(amino acids) and its calcium sulfate/calcium hydrogen phosphate composites: Reducing oxidative stress and promoting osteogenesis.","authors":"Wei Zhang, Xiaolu Chen, Hao Deng, Xinyue Yang, Shijie Cai, Hulin Yang, Haohao Ren, Yonggang Yan","doi":"10.1016/j.colsurfb.2024.114485","DOIUrl":"https://doi.org/10.1016/j.colsurfb.2024.114485","url":null,"abstract":"<p><p>The imbalance of redox homeostasis, especially the abnormal levels of reactive oxygen species (ROS), is a key obstacle in the bone repair process. Therefore, developing materials capable of scavenging ROS and modulating the microenvironment of bone defects is crucial for promoting bone repair. In this study, to endow poly(amino acids) (PAA) and its composites with anti-oxidative stress properties and enhanced osteogenic differentiation, we designed and prepared a calcium sulfate/calcium hydrogen phosphate/poly(amino acids) (PCDM) composite material with a thioether structure (-S-) in the molecular chain of PAA matrix through situ polymerization and physical blending method. The results showed that the thioether was successfully introduced into the polymer, and the intrinsic viscosities of the poly(amino acids) ranged from 0.27 to 0.73 dL/g. PCDM materials exhibited good mechanical properties, with a compressive strength ranging from 16.28 to 33.83 MPa. The degradation performance results showed that the composite materials had a weight loss of 23.9-35.3 % after four weeks. The antioxidant stress results showed that the PCDM composite materials scavenged 67.6 %-78.3 % of DPPH radicals after 24 h and 61.4 %-93.6 % of ABTS radicals after 4 h, effectively reducing ROS levels in mouse bone mesenchymal stem cells. The cytotoxicity and osteogenic differentiation results showed that the materials had cytocompatibility and could promote alkaline phosphatase secretion and mineralized nodule formation. In conclusion, PCDM materials might broaden the application of poly(amino acids) composites in bone defect repair by regulating the ROS microenvironment and promoting the osteogenic differentiation of stem cells.</p>","PeriodicalId":279,"journal":{"name":"Colloids and Surfaces B: Biointerfaces","volume":"248 ","pages":"114485"},"PeriodicalIF":5.4,"publicationDate":"2024-12-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142925852","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Anticancer therapeutic effect of magnetic guided cobalt ferrite/doxorubicin-loaded ROS-responsive bilirubin nanoparticles in a colon cancer model.","authors":"Hyo Kang, Reju George Thomas, Subin Kim, Jae Kyun Ju, Yong Yeon Jeong","doi":"10.1016/j.colsurfb.2024.114487","DOIUrl":"https://doi.org/10.1016/j.colsurfb.2024.114487","url":null,"abstract":"<p><strong>Purpose: </strong>The aim of this study is to synthesize the cobalt iron oxide (CoFe) and doxorubicin (Dox)-loaded chitosan bilirubin (ChiBil) nanoparticles and to investigate the anticancer therapeutic effect of the synthesized nanoparticles under magnetic guidance in a colon cancer.</p><p><strong>Materials and methods: </strong>ChiBil-CoFe-Dox nanoparticles were synthesized by conjugating CoFe and Dox and then loaded onto ChiBil nanoparticles. Synthesis were characterized using thermogravimetric (TGA) analysis, inductive coupled plasma (ICP) analysis, dynamic light scattering (DLS), zeta potential and field emission-transmission electron microscopy (FE-TEM). Cellular uptake and cytotoxicity studies were conducted in vitro. Biodistribution and tumor inhibition study was done in vivo CT-26 colon cancer model.</p><p><strong>Results: </strong>The ChiBil-CoFe-Dox nanoparticles were successfully synthesized in this study. The in vitro cytotoxicity study showed that the ChiBil-CoFe-Dox nanoparticle had a toxic effect on cancer cells. The accumulation of ChiBil-CoFe-Dox nanoparticles was enhanced under magnetic guidance, as observed by in vivo. Tumor inhibition study showed that the ChiBil-CoFe-Dox nanoparticle effectively reduced tumor size in vivo mice colon cancer model, especially when combined with magnetic guidance.</p><p><strong>Conclusion: </strong>This study showed that ChiBil-CoFe-Dox nanoparticle was successfully synthesized and effectively reduced tumor size, especially when combined with magnetic guidance. The in vitro and in vivo results suggested that the ROS stimuli responsive ChiBil-CoFe-Dox nanoparticles may be a potent therapeutic option for treating colon cancer.</p>","PeriodicalId":279,"journal":{"name":"Colloids and Surfaces B: Biointerfaces","volume":"248 ","pages":"114487"},"PeriodicalIF":5.4,"publicationDate":"2024-12-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142930217","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Construction of a high-capacity drug microcarrier using diatom frustules.","authors":"Houjie Wang, Runyu You, Yangqi Jin, Guangning Wang, Feng Li, Yahui Gao, Changping Chen, Nengming Xiao, Junrong Liang","doi":"10.1016/j.colsurfb.2024.114481","DOIUrl":"https://doi.org/10.1016/j.colsurfb.2024.114481","url":null,"abstract":"<p><p>The drug loading capacity is a critical performance metric for drug delivery systems. A high capacity ensures efficient drug delivery to target sites at lower doses, reducing the amount of carrier material needed and lessening patient burden. However, improving drug loading capacity in diatom frustule-based systems remains a challenge. In this study, we explored effective strategies for developing a microcarrier with a high drug loading efficiency using diatom frustules (DF) derived from Thalassiosira weissflogii. We found that combining an evaporative loading method with a chitosan (Chi) coating was particularly effective for enhancing the drug loading capacity of indomethacin (IND), a hydrophobic model drug. Further optimization of the indomethacin-to-APTES-modified frustule (DF-NH₂) ratio to 2:1, along with adjusting the medium pH to 5, further improved drug loading efficiency. Additionally, the chitosan coating on the drug-loaded frustules not only enabled sustained drug release but also enhanced the biocompatibility of the carriers. The resulting DF-NH₂/IND@Chi microcarrier demonstrated a drug loading efficiency of 58.78 ± 1.92 % for IND, with a pH-dependent controlled release profile. This performance significantly outperforms previous reports, which typically report loading efficiencies between 10 % and 35 %, with few exceeding 40 %. In vitro cytotoxicity tests also revealed significant activity against colon cancer cells, highlighting the potential therapeutic benefits of this system. This study provides a systematic approach to creating high-capacity drug microcarriers using diatom frustules, offering promising prospects for future drug delivery applications.</p>","PeriodicalId":279,"journal":{"name":"Colloids and Surfaces B: Biointerfaces","volume":"248 ","pages":"114481"},"PeriodicalIF":5.4,"publicationDate":"2024-12-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142913222","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"4D bioprinting of transformable living constructs with sustained local growth factor presentation for advanced tissue engineering applications.","authors":"Guodong Wu, Lin Wang, Yuhang Cao, Manli Wang, Chun Yang, Jian Zhang","doi":"10.1016/j.colsurfb.2024.114484","DOIUrl":"https://doi.org/10.1016/j.colsurfb.2024.114484","url":null,"abstract":"<p><p>Traditional tissue engineering strategies focus on geometrically static tissue scaffolds, lacking the dynamic capability found in native tissues. The emerging field of 4D bioprinting offers a promising method to address this challenge. However, the requirement for consistent exogenous supplementation of growth factors (GFs) during tissue maturation poses a significant obstacle for in vivo application of 4D bioprinted constructs. We herein developed composite bioinks composed of photocrosslinkable, jammed alginate methacrylate (AlgMA) and gelatin methacrylate (GelMA), incorporating GelMA microspheres loaded with GFs to provide sustained local GF presentation over 50 days for 4D tissue bioprinting. The composite bioink exhibited excellent printability, enabling 3D printing with good accuracy (∼120 %) and fidelity (105 % - 114 %). By incorporating a photoabsorbent to enhance light attenuation, a gradient network along the light propagation pathway was generated, facilitating programmable and controllable 4D shape transformation. This process allowed the fabrication of complex living constructs with defined architectures through morphing. A proof-of-concept study on cartilage regeneration demonstrated the effectiveness of sustained GF presentation in driving tissue development, showing significant glycosaminoglycan production (GAG/DNA 10.3), and substantial upregulation of type II collagen (125.8-fold) and aggrecan (16.4-fold) mRNA expression, thereby eliminating the need for exogenous GF supplementation. This study underscores the transformative potential of integrating dynamic tissue scaffolding with sustained GF delivery, thereby addressing key limitations of traditional tissue engineering approaches and offering new avenues for tissue repair applications.</p>","PeriodicalId":279,"journal":{"name":"Colloids and Surfaces B: Biointerfaces","volume":"248 ","pages":"114484"},"PeriodicalIF":5.4,"publicationDate":"2024-12-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142908894","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}