Colloids and Surfaces B: Biointerfaces最新文献

{"title":"Disrupting Cdc42 activation-driven filopodia formation with low-intensity ultrasound and microbubbles: A novel strategy to block ovarian cancer metastasis","authors":"Xiaoying Li ,&nbsp;Chengwei Tan ,&nbsp;Xiuxiu Fu ,&nbsp;Jian Qiu ,&nbsp;Wanting Shen ,&nbsp;Zhikang Xu ,&nbsp;Xiaodong Wu ,&nbsp;Yiting Zhou ,&nbsp;Xiao Li ,&nbsp;Litao Sun ,&nbsp;Jiale Qin","doi":"10.1016/j.colsurfb.2025.114724","DOIUrl":"10.1016/j.colsurfb.2025.114724","url":null,"abstract":"<div><div>Metastasis is a primary cause of mortality and treatment failure in ovarian cancer, with limited effective therapeutic strategies. Low-intensity ultrasound (LIUS) and microbubbles (MBs) has been demonstrated as an adjunctive technique capable of enhancing drug delivery and suppressing tumor metastasis. However, the underlying mechanisms remain incompletely understood. In this study, we aimed to investigate whether LIUS + MBs alone could suppress tumor metastasis and to explore its mechanism of action through disruption of the cytoskeletal remodeling in filopodia, an essential structure in the early stages of cancer cell dissemination. Based on cell-based experiments to determine the optimal parameters, our results showed LIUS + MBs significantly inhibited the migration and invasion of ovarian cancer cells. <em>In vivo</em>, LIUS + MBs treatment markedly suppressed the overall metastasis in the orthotopic ovarian cancer model, and in both the intraperitoneal and hematogenous metastatic models established by injecting pretreated cells. Morphologically, such treatment led to a notable reduction in the length and number of filopodia, while the number of lamellipodia remained unaffected. At the molecular level, LIUS + MBs disturbed filopodia formation and the metastatic potential of ovarian cancer cells by suppressing the activation of Cdc42, a key regulator of cytoskeletal dynamics. The inhibitory effect was reversed by the overexpression of Cdc42CA. Further proteomic and bioinformatics analysis implied that LIUS + MBs may reduce Cdc42 activity by upregulating the expression of GTPase-activating proteins (GAPs). Our research provides novel insight into the mechanism by which LIUS + MBs can inhibit tumor metastasis, highlighting its role in disturbing the Cdc42-mediated cytoskeletal remodelling of filopodia.</div></div>","PeriodicalId":279,"journal":{"name":"Colloids and Surfaces B: Biointerfaces","volume":"253 ","pages":"Article 114724"},"PeriodicalIF":5.4,"publicationDate":"2025-04-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143883271","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Towards understanding particle-protein complexes: Physicochemical, structural, and cellbiological characterization of β-lactoglobulin interactions with silica, polylactic acid, and polyethylene terephthalate nanoparticles","authors":"Helena Kieserling ,&nbsp;Holger Sieg ,&nbsp;Jasmin Heilscher ,&nbsp;Stephan Drusch ,&nbsp;Albert Braeuning ,&nbsp;Andreas F. Thünemann ,&nbsp;Sascha Rohn","doi":"10.1016/j.colsurfb.2025.114702","DOIUrl":"10.1016/j.colsurfb.2025.114702","url":null,"abstract":"<div><div>Nanoplastic particles and their additives are increasingly present in the food chain, interacting with biomacromolecules with not yet known consequences. A protein corona forms around the particles in these usually complex matrices, primarily with a first contact at surface-active proteins. However, systematic studies on the interactions between the particles and proteins –especially regarding protein affinity and structural changes due to surface properties like polarity – are limited. It is also unclear whether the protein corona can \"mask\" the particles, mimic protein properties, and induce cytotoxic effects when internalized by mammalian cells. This study aimed at investigating the physicochemical properties of model particle-protein complexes, the structural changes of adsorbed proteins, and their effects on Caco-2 cells. Whey protein β-lactoglobulin (β-Lg) was used as a well-characterized model protein and studied in a mixture with nanoparticles of varying polarity, specifically silica, polylactic acid (PLA), and polyethylene terephthalate (PET). The physicochemical analyses included measurements of the hydrodynamic diameter and the zeta potential, while the protein conformational changes were analyzed using Fourier-transform-infrared spectroscopy (FTIR) and intrinsic fluorescence. Cellular uptake in Caco-2 cells was assessed through flow cytometry, cell viability was measured using the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium-bromide (MTT) assay, and cellular impedance was analyzed with xCELLigence® technology. The results indicated that β-Lg had the highest affinity for hydrophilic silica particles, forming silica-β-Lg complexes and large aggregates through electrostatic interactions. The affinity decreased for PLA and was lowest for hydrophobic PET, which formed smaller complexes. Adsorption onto silica caused partial unfolding and refolding of β-Lg. The silica-β-Lg complexes were internalized by Caco-2 cells, impairing cell proliferation. In contrast, PLA- and PET-protein complexes were not internalized, though PLA complexes slightly reduced cell viability. This study enhances our understanding of protein adsorption on nanoparticles and its potential biological effects.</div></div>","PeriodicalId":279,"journal":{"name":"Colloids and Surfaces B: Biointerfaces","volume":"253 ","pages":"Article 114702"},"PeriodicalIF":5.4,"publicationDate":"2025-04-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143883270","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Polydopamine-assisted dual metal ion modification of titanium: Enhancing osseointegration and antibacterial performance","authors":"Jingyang Kang ,&nbsp;Sikun Meng ,&nbsp;Chenhui Liu ,&nbsp;Huachun Wang ,&nbsp;Tianzhen Zhang ,&nbsp;Cheng Qi ,&nbsp;Mei Li","doi":"10.1016/j.colsurfb.2025.114717","DOIUrl":"10.1016/j.colsurfb.2025.114717","url":null,"abstract":"<div><div>Titanium (Ti) implants are widely used for tooth replacement due to their exceptional mechanical properties and high biocompatibility. However, their inherently inert surface limits osteogenic potential and makes them prone to bacterial colonization, increasing the risk of biofilm formation and implant-related infections. To address these limitations, surface modification of Ti is essential. This study aimed to enhance the surface properties of Ti by coating it with polydopamine (PDA) and further doping it with copper and calcium ions. TPDA was prepared and subsequently used to fabricate TPDA@Cu and TPDA@CuCa samples. Material characterization confirmed that TPDA@CuCa exhibited excellent surface wettability and biocompatibility, with Cu^2 + and Ca²⁺ being continuously and stably released in liquid environments. Additionally, TPDA@CuCa significantly improved protein adsorption, facilitating favorable cellular interactions. In vitro experiments demonstrated that TPDA@CuCa exhibited strong antimicrobial activity against Escherichia coli and Staphylococcus aureus, enhanced osteoblast adhesion, mineralization, and upregulated osteogenic gene expression. This bifunctional surface modification strategy offers a promising approach to enhancing both the osteogenic and antibacterial properties of Ti implants.</div></div>","PeriodicalId":279,"journal":{"name":"Colloids and Surfaces B: Biointerfaces","volume":"253 ","pages":"Article 114717"},"PeriodicalIF":5.4,"publicationDate":"2025-04-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143883269","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Smart self-assembled peptide-based hydrogels: Mechanism, design and biomedical applications","authors":"Tao Ma ,&nbsp;Yi Yu ,&nbsp;Yijun Gao ,&nbsp;Shanshan Jiang ,&nbsp;Wenhui Ge ,&nbsp;Yiyu Zeng ,&nbsp;Xinying Wang ,&nbsp;Shuangjiang Li ,&nbsp;Xiaoyan Xie ,&nbsp;Gaopeng Guan","doi":"10.1016/j.colsurfb.2025.114704","DOIUrl":"10.1016/j.colsurfb.2025.114704","url":null,"abstract":"<div><div>Peptide hydrogels have gained widespread attention in biomedical engineering due to their unique ability to mimic the cellular microenvironment in vivo. Stimulus-responsive self-assembled (SAP) hydrogels can undergo conformational changes in response to changes in the external environment, prompting a sol-gel transition. Their inherent biodegradability, excellent surface activity and biocompatibility make them ideal candidates for a wide range of biomedical applications, and these SAP hydrogels can be widely used in the fields of tissue engineering, cell and drug delivery, wound healing and medical diagnostic imaging. In this paper, the basic properties, design principles, preparation methods and self-assembly mechanisms of different types of stimuli-responsive SAP hydrogels are reviewed. By designing and constructing stimulus-responsive SAP hydrogels, we can create materials that mimic natural physiological environments, thereby better simulating cell behavior and tissue repair. In addition, it highlights specific applications of these hydrogels in biomedical engineering, supported by examples from recent literature. The report summarizes the current state of research, highlights key challenges, and provides insights into future prospects to encourage continued innovation and exploration in this rapidly evolving field.</div></div>","PeriodicalId":279,"journal":{"name":"Colloids and Surfaces B: Biointerfaces","volume":"253 ","pages":"Article 114704"},"PeriodicalIF":5.4,"publicationDate":"2025-04-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143879422","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Molecular insights into the controlled release process of cyclodextrin-resveratrol inclusion complexes in the stratum corneum","authors":"Xindong Yu ,&nbsp;Shasha Liu ,&nbsp;Ying Li ,&nbsp;Shiling Yuan","doi":"10.1016/j.colsurfb.2025.114725","DOIUrl":"10.1016/j.colsurfb.2025.114725","url":null,"abstract":"<div><div>Cyclodextrins (CDs) are efficient drug carriers for improving drug solubility, stability, and bioavailability. However, the mechanism underlying the interaction between cyclodextrin-drug inclusion complexes and skin remains unclear. In this work, molecular simulations were employed to study the release process of cyclodextrin-resveratrol inclusion complexes on the surface of the lipid bilayer. The results showed that structural orientation significantly influences release kinetics. Resveratrol (RES) is able to form inclusion complexes with β-CD in two possible orientations: M-form (Mono-hydroxyl group toward the primary rim of β-CD) and D-form (Di-hydroxyl group toward the secondary rim of β-CD). M-form inclusion structures facilitated RES release more efficiently than D-form configurations. Cavity-specific lipid interactions are the dominant driver of the release process. Meanwhile, it was determined that the β-CD/RES inclusion complex exhibited greater stability than γ-CD/RES and demonstrated superior release efficiency at the lipid membrane surface in comparison to α-CD/RES. This suggests that the cavity size of β-CD is more suitable for delivering resveratrol. Furthermore, umbrella sampling simulations reveal that hydroxypropyl-substituted β-CD could lessen the irritation to the lipid bilayer. The present study provides a theoretical foundation for the rational design of CD-based drug delivery systems.</div></div>","PeriodicalId":279,"journal":{"name":"Colloids and Surfaces B: Biointerfaces","volume":"253 ","pages":"Article 114725"},"PeriodicalIF":5.4,"publicationDate":"2025-04-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143863535","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Understanding the transport of drugs across biomimetic barriers of various phospholipid compositions using a combined experimental and computational approach","authors":"Aleksei Kabedev ,&nbsp;Mikkel Højmark Tønning ,&nbsp;Alexandra Teleki ,&nbsp;Annette Bauer-Brandl ,&nbsp;Ann-Christin Jacobsen","doi":"10.1016/j.colsurfb.2025.114706","DOIUrl":"10.1016/j.colsurfb.2025.114706","url":null,"abstract":"<div><div>Permeapad® is an artificial biomimetic barrier for in vitro permeation experiments, which has an intricate nano- and microstructure consisting of two cellulose hydrate sheets enclosing a layer of phospholipids forming multiple, multilamellar vesicles in contact with the assay medium. Due to this structure, transport across this barrier can be regarded as complex deserving further attention. Until now, only Permeapad® with phosphatidylcholine, the most abundant phospholipid in cell membranes, has been described in literature. However, from biological systems and other artificial barriers, it is known that permeation properties can vary with phospholipid composition. This study presents a combination of experimental and computational techniques to study and explain the transport of molecules across the Permeapad® barrier. For this, we investigated Permeapad® variants with other phospholipid compositions including phosphatidylethanolamine, the second most abundant phospholipid in cell membranes, and phosphatidylglycerol, representing a phospholipid with a negatively charged headgroup by measuring the permeability of three drugs, metoprolol (a weak base), naproxen (a weak acid) and hydrocortisone (a non-ionizable drug). Phospholipid composition only affected the permeability of metoprolol significantly. We used molecular dynamics simulations to understand the underlying mechanisms of the permeability differences extracting several descriptors of membrane properties and predicting permeability. Surprisingly, an almost inverse relationship between experimental and computational permeability was observed. Permeapad®'s highly compartmentalized structure was hypothesized to cause this observation. This study offers a deeper understanding of the functionality of the Permeapad® barrier.</div></div>","PeriodicalId":279,"journal":{"name":"Colloids and Surfaces B: Biointerfaces","volume":"253 ","pages":"Article 114706"},"PeriodicalIF":5.4,"publicationDate":"2025-04-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143890908","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Galectin-1-targeted theranostic nanoprobe for multimodal imaging and photothermal therapy in cervical cancer lymph node metastases","authors":"Hui Duan ,&nbsp;Huimin Li ,&nbsp;Qianjun Su ,&nbsp;Ping Liu ,&nbsp;Mengdan Zhang ,&nbsp;Xiaolin Chen ,&nbsp;Ruiyuan Liu ,&nbsp;Pengfei Li ,&nbsp;Chunlin Chen","doi":"10.1016/j.colsurfb.2025.114714","DOIUrl":"10.1016/j.colsurfb.2025.114714","url":null,"abstract":"<div><div>Accurately evaluating lymph node (LN) status is essential for cervical cancer staging and making treatment decisions. However, the current preoperative imaging modalities for diagnosing LN metastasis in patients with cervical cancer are suboptimal, and the existing radiochemotherapy methods lack specificity, which leads to increased toxicity and side effects. To overcome these limitations, we aimed to develop a theranostic strategy for LN metastases with a galectin-1-targeted nanoprobe for dual-modal magnetic resonance imaging (MRI)/near-infrared (NIR) imaging and photothermal therapy (PTT). The PEG@MnPb-IR820 nanoparticles were synthesized through an integrated approach combining microemulsion, thin-film hydration, and esterification coupling methods. <em>The nanoparticles were synthesized and characterized for their physicochemical properties and biosafety</em>. After extensive in vitro and in vivo experiments, the TDG@PMI NPs demonstrated low biological toxicity, excellent stability, and a diameter of 74 nm, which is suitable for LN accumulation. With effective MRI and NIR imaging guidance, this nanoprobe displayed outstanding anticancer activity in tumors and metastatic sentinel LNs upon single NIR laser irradiation by inducing mitochondrial apoptosis in tumor cells.</div></div>","PeriodicalId":279,"journal":{"name":"Colloids and Surfaces B: Biointerfaces","volume":"253 ","pages":"Article 114714"},"PeriodicalIF":5.4,"publicationDate":"2025-04-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143863534","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A multifunctional nanoplatform based on chlorin e6 for fluorescence imaging-guided sonodynamic and chemodynamic cancer therapy","authors":"Qi Xu ,&nbsp;Fang He ,&nbsp;Zhimin Mo ,&nbsp;Han Hu ,&nbsp;Qianyuan He ,&nbsp;Zushun Xu ,&nbsp;Shengli Yang ,&nbsp;Xiaojuan Qin","doi":"10.1016/j.colsurfb.2025.114718","DOIUrl":"10.1016/j.colsurfb.2025.114718","url":null,"abstract":"<div><div>Emerging multimodal therapy strategies based on sonodynamic therapy (SDT) are foreseen as foundational in forthcoming tumor theranostics. Nevertheless, the therapeutic effects of SDT are limited by the hypoxic environment of the tumor microenvironment (TME). Based on this, nano-enzymes are envisioned to decompose excess hydrogen peroxide in the tumor microenvironment to generate oxygen. Here, PEG-PLGA-coated CeO₂ nanoparticles loaded with the sonosensitizer chlorin e6 (CC@PP) were prepared for cancer therapy based on a nanoprecipitation method. CC@PP possesses highly efficient catalase-like (CAT) properties, which are able to decompose overexpressed H₂O₂ into O₂ and enhance the effect of O₂-dependent sonodynamic therapy. In addition, it can act as a peroxidase (POD) at lower pH for chemodynamic therapy. Under the guidance of the US, CC@PP can perform synergistic sonodynamic and chemodynamic cancer therapy and inhibit tumor growth efficiently, CC@PP is biocompatible, has good <em>in vivo</em> fluorescence imaging effect, and has integrated diagnostic and therapeutic properties, which can be effectively aggregated at the tumor area. In this study, the effect of sonodynamic therapy was enhanced by improving the hypoxic environment in the TME, and multimodal synergistic enhancement of cancer therapy was realized.</div></div>","PeriodicalId":279,"journal":{"name":"Colloids and Surfaces B: Biointerfaces","volume":"253 ","pages":"Article 114718"},"PeriodicalIF":5.4,"publicationDate":"2025-04-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143859859","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Immunomodulatory peptide DP7-C mediates macrophage-derived exosomal miR-21b to promote bone regeneration via the SOCS1/JAK2/STAT3 axis","authors":"Shuang Lai ,&nbsp;Na Tang ,&nbsp;Jun Guo ,&nbsp;Li Deng ,&nbsp;Lun Yuan ,&nbsp;Linya Zeng ,&nbsp;Lu Yang ,&nbsp;Yandong Mu","doi":"10.1016/j.colsurfb.2025.114709","DOIUrl":"10.1016/j.colsurfb.2025.114709","url":null,"abstract":"<div><div>Periodontitis, the most prevalent chronic inflammatory disease leading to bone resorption, presents significant challenges for achieving optimal periodontal bone regeneration and repair despite efforts to reduce inflammation and stimulate osteogenesis. Macrophage-derived exosomes have emerged as promising therapeutic agents due to their osteogenic and immunomodulatory potential. Specific stimulation of macrophages can alter the exosomal composition, particularly microRNAs (miRNAs), thereby altering their functions. DP7-C, a cationic immunomodulatory peptide, is known to regulate immune responses and cellular processes by interacting with cell membranes and signaling pathways. However, its effects on macrophage exosomal miRNA profiles remain poorly understood. In this study, we identified differential miRNA expression in macrophage-derived exosomes following DP7-C stimulation, with a notable upregulation of miR-21b. To investigate the osteogenic role of exosomal miR-21b, DP7-C was utilized to facilitate the transfection of miR-21b into macrophages, leading to the secretion of exosomes enriched with miR-21b. These exosomes enhanced osteogenic differentiation <em>in vitro</em> and alleviated periodontal tissue damage in an experimental periodontitis model <em>in vivo</em>. Mechanistically, exosomal miR-21b promotes osteogenesis by directly targeting the suppressor of cytokine signaling (SOCS1), thereby activating the JAK2/STAT3 signaling pathway. This study establishes macrophage-derived exosomal miR-21b as a potent catalyst for bone regeneration, highlighting a promising acellular therapeutic strategy for periodontitis.</div></div>","PeriodicalId":279,"journal":{"name":"Colloids and Surfaces B: Biointerfaces","volume":"253 ","pages":"Article 114709"},"PeriodicalIF":5.4,"publicationDate":"2025-04-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143874510","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Tannic acid-etched PAN/PVP nanofibers loaded with Cu-MOFs enhance antibacterial efficacy and accelerate wound healing","authors":"Zhenyu Wang ,&nbsp;Jianxiang Li ,&nbsp;Sihan Li ,&nbsp;Diwei Li ,&nbsp;Yunyi Zhao ,&nbsp;Ling Xu ,&nbsp;Gang Liu ,&nbsp;Zhaoqiong Chen ,&nbsp;Xiaoming Luo","doi":"10.1016/j.colsurfb.2025.114719","DOIUrl":"10.1016/j.colsurfb.2025.114719","url":null,"abstract":"<div><div>Wound infection represents a prevalent and pressing clinical challenge, resulting in delayed wound healing and severe complications. In this study, a novel wound dressing was fabricated through a combination of electrospinning and acid etching techniques. First, nanofibers were fabricated by blending electrospun polyacrylonitrile/Polyvinylpyrrolidone (PAN/PVP) polymers with copper-based metal organic frameworks (Cu-MOFs). Subsequently, tannic acid was employed to etch the surface sites of Cu-MOFs on the fibers. The obtained nanofibers exhibited a typical porous structure, superior water absorption and gas permeability, with the average water vapor transmission rate was 2170.6 gm^-2day^-1. Additionally, the release behavior of copper ions can be modulated by altering the mass ratio of PVP to PAN, the amount of Cu-MOFs and the use of tannic acid. <em>In vitro</em> antibacterial assays revealed that the antibacterial efficacy of nanofibers increased with the addition of Cu-MOFs, after 48 hours of treatment, the inhibition rates of the nanofibers against <em>E. coli</em> and <em>S. aureus</em> reached over 79.5 % and 90 %, respectively. <em>In vivo</em> experiments demonstrated that these nanofibers alleviated wound inflammation and promoted collagen and angiogenesis, exhibition superior anti-inflammatory and wound-healing effects. The biosafety tests indicated that the nanofibers loaded with 1 % and 3 % Cu-MOFs exhibited good biocompatibility, while the nanofibers loaded with 5 % Cu-MOFs showed slight cytotoxicity. This study provides a novel strategy for the design and fabrication of advanced wound dressings in biomedical applications.</div></div>","PeriodicalId":279,"journal":{"name":"Colloids and Surfaces B: Biointerfaces","volume":"253 ","pages":"Article 114719"},"PeriodicalIF":5.4,"publicationDate":"2025-04-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143855698","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}