Colloids and Surfaces B: Biointerfaces最新文献_第5页

Paper immobilized BSA-decorated gold nanoclusters for single-step optical sensing of glucose and cholesterol without cross-reactivity. 纸张固定 BSA 装饰金纳米团簇，用于单步光学检测葡萄糖和胆固醇，无交叉反应。

IF 5.4 2区医学

Colloids and Surfaces B: Biointerfaces Pub Date : 2025-01-01 Epub Date: 2024-10-09 DOI: 10.1016/j.colsurfb.2024.114303

M R Krishnendu, Stuti Bhagat, Vidhi Jain, Divya Mehta, Sanjay Singh

{"title":"Paper immobilized BSA-decorated gold nanoclusters for single-step optical sensing of glucose and cholesterol without cross-reactivity.","authors":"M R Krishnendu, Stuti Bhagat, Vidhi Jain, Divya Mehta, Sanjay Singh","doi":"10.1016/j.colsurfb.2024.114303","DOIUrl":"10.1016/j.colsurfb.2024.114303","url":null,"abstract":"Minimally invasive methods for detecting glucose, cholesterol and hydrogen peroxide are crucial for monitoring the nutritional and health status of humans and animals. The peroxidase mimetic activity by nanozymes is one of the versatile methods for detecting glucose, cholesterol, hydrogen peroxide, and other biomolecules. However, the strict requirement of acidic pH limits their sensing and interfacing ability with natural enzymes. The present study developed bovine serum albumin (BSA) coated gold nanoclusters (AuNC) immobilized on paper fabric to enable single-step visual detection of glucose, cholesterol and hydrogen peroxide in complex biological fluids like serum and milk. The BSA-AuNC suspension and immobilized paper fabric synergistically interface with the natural oxidative enzymes, glucose oxidase or cholesterol oxidase, at physiological pH. The concomitant loss in the fluorescent intensity of BSA-AuNC-loaded paper fabric exposed to the generated hydrogen peroxide (glucose/glucose oxidase or cholesterol/cholesterol oxidase) was directly proportional to the concentration of glucose or cholesterol. These reactions enabled simple visual detection as well as quantification of hydrogen peroxide, glucose and cholesterol using Image-J software and common smartphone-based mobile applications. The detection ability of BSA-AuNC-embedded paper fabric is specific and remains unaltered in the presence of similar oxidase enzymes or similar substrate analogues. With these unique features, the BSA-AuNC embedded paper fabric stands out as a prominent analytical device with enormous potential as a simple, user-friendly detection tool for monitoring biomolecules that are important to health, nutrition, and environmental safeguarding.","PeriodicalId":279,"journal":{"name":"Colloids and Surfaces B: Biointerfaces","volume":"245 ","pages":"114303"},"PeriodicalIF":5.4,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142454511","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Physico-chemical properties and biological evaluation of graphene quantum dots for anticancer drug susceptibility. 石墨烯量子点抗癌药物敏感性的物理化学特性和生物学评价。

IF 5.4 2区医学

Colloids and Surfaces B: Biointerfaces Pub Date : 2025-01-01 Epub Date: 2024-10-15 DOI: 10.1016/j.colsurfb.2024.114322

Nirupam Das, Ravishankar Srivastava, Sawna Roy, Arup K De, Rajiv K Kar

{"title":"Physico-chemical properties and biological evaluation of graphene quantum dots for anticancer drug susceptibility.","authors":"Nirupam Das, Ravishankar Srivastava, Sawna Roy, Arup K De, Rajiv K Kar","doi":"10.1016/j.colsurfb.2024.114322","DOIUrl":"10.1016/j.colsurfb.2024.114322","url":null,"abstract":"Graphene quantum dots (GQDs) possess unique optical and biocompatible properties, making them suitable candidates for biomedical and pharmaceutical applications. This study reports the hydrothermal synthesis of pristine-GQD and doped variants: Nitrogen-GQD and Sulfur-GQD. The materials underwent thorough characterization techniques such as UV-vis, fluorescence, XRD, FE-TEM/SEM, EDX, and Raman spectroscopy. The particle sizes of these GQDs range from 2 to 5 nm. We conducted a comprehensive study through MTT assays to evaluate the potential cytotoxic effect of GQD and the doped variants. This study demonstrated their synergistic interactions with an anti-cancer drug, methotrexate (MTX), and also improvement of cytocompatibility in the presence of folic acid (FA). Systematic MD simulations revealed a compacting effect on the dynamic behavior of GQD and its variants in the presence of drugs. Fluorescence spectroscopy and computational modeling suggest non-intercalative surface interactions between GQDs and the drugs. The cytotoxic activity of pristine GQD on HeLa cervical cancer cells is higher than that of N-GQD and S-GQD. When treated with GQD-IC50-MTX-IC50, only 5.6 % of HeLa cells remained viable. The doped variants exhibited bio-compatibility when tested on normal HEK cell lines. Overall, this study emphasizes the potential of GQDs for targeted cancer therapy through an interdisciplinary approach involving material characterization, computational modeling, and biological assays.","PeriodicalId":279,"journal":{"name":"Colloids and Surfaces B: Biointerfaces","volume":"245 ","pages":"114322"},"PeriodicalIF":5.4,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142454512","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Multifunctional injectable oxidized sodium alginate/carboxymethyl chitosan hydrogel for rapid hemostasis. 用于快速止血的多功能注射用氧化海藻酸钠/羧甲基壳聚糖水凝胶。

IF 5.4 2区医学

Colloids and Surfaces B: Biointerfaces Pub Date : 2025-01-01 Epub Date: 2024-10-29 DOI: 10.1016/j.colsurfb.2024.114346

Xuanyu Liu, Junjie Hu, Yinchun Hu, Yeying Liu, Yan Wei, Di Huang

{"title":"Multifunctional injectable oxidized sodium alginate/carboxymethyl chitosan hydrogel for rapid hemostasis.","authors":"Xuanyu Liu, Junjie Hu, Yinchun Hu, Yeying Liu, Yan Wei, Di Huang","doi":"10.1016/j.colsurfb.2024.114346","DOIUrl":"10.1016/j.colsurfb.2024.114346","url":null,"abstract":"Uncontrolled bleeding from incompressible or irregularly shaped wounds is a major factor in the death of people in the battlefield or surgery process. Ideal rapid hemostatic materials should have the performance of rapid hemostasis and at the same time can be applied to a variety of complex wound trauma types, in addition, excellent antimicrobial properties, adhesion, biocompatibility, degradation, and the non-toxicity of degradation products are also necessary, but there are fewer hemostatic materials that meet these requirements. Herein, we prepared an injectable hemostatic hydrogel based on the natural products sodium alginate (SA) and carboxymethyl chitosan (CMC). Oxidized sodium alginate (OSA) was prepared by the oxidation reaction of NaIO4 with SA, and OSA with aldehyde group was mixed with CMC with amino group to rapidly form an in situ injectable hemostatic hydrogel (OSA/CMC) by the Schiff base reaction. OSA/CMC hydrogel exhibited excellent antimicrobial and adhesion properties by the Schiff base reaction. In addition, OSA/CMC hydrogel directly activate the endogenous coagulation pathway through the synergistic effect of OSA, CMC to enhance the hemostatic effect. The results of in vivo hemostasis study showed that OSA/CMC hydrogel significantly accelerated hemostasis and reduced blood loss in liver hemorrhage model and tail amputation model. Therefore, OSA/CMC hydrogel is expected to be a potential material in the direction of rapid clinical hemostasis due to its good adhesion properties, antimicrobial properties, biocompatibility, blood compatibility, and efficient rapid hemostasis.","PeriodicalId":279,"journal":{"name":"Colloids and Surfaces B: Biointerfaces","volume":"245 ","pages":"114346"},"PeriodicalIF":5.4,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142563524","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Synthesis of dumbbell-like heteronanostructures encapsulated in ferritin protein: Towards multifunctional protein based opto-magnetic nanomaterials for biomedical theranostic. 铁蛋白包裹的哑铃状异质结构的合成：开发基于蛋白质的多功能光磁纳米材料，用于生物医学治疗。

IF 5.4 2区医学

Colloids and Surfaces B: Biointerfaces Pub Date : 2025-01-01 Epub Date: 2024-10-24 DOI: 10.1016/j.colsurfb.2024.114332

Italo Moglia, Margarita Santiago, Andreas Arellano, Sebastián Salazar Sandoval, Álvaro Olivera-Nappa, Marcelo J Kogan, Mónica Soler

{"title":"Synthesis of dumbbell-like heteronanostructures encapsulated in ferritin protein: Towards multifunctional protein based opto-magnetic nanomaterials for biomedical theranostic.","authors":"Italo Moglia, Margarita Santiago, Andreas Arellano, Sebastián Salazar Sandoval, Álvaro Olivera-Nappa, Marcelo J Kogan, Mónica Soler","doi":"10.1016/j.colsurfb.2024.114332","DOIUrl":"10.1016/j.colsurfb.2024.114332","url":null,"abstract":"Dumbbell-like hetero nanostructures based on gold and iron oxides is a promising material for biomedical applications, useful as versatile theranostic agents due the synergistic effect of their optical and magnetic properties. However, achieving precise control on their morphology, size dispersion, colloidal stability, biocompatibility and cell targeting remains as a current challenge. In this study, we address this challenge by employing biomimetic routes, using ferritin protein nanocages as template for these nanoparticles' synthesis. We present the development of an opto-magnetic nanostructures using the ferritin protein, wherein gold and iron oxide nanostructures were produced within its cavity. Initially, we investigated the synthesis of gold nanostructures within the protein, generating clusters and plasmonic nanoparticles. Subsequently, we optimized the conditions for the superparamagnetic nanoparticles synthesis through controlled iron oxidation, thereby enhancing the magnetic properties of the resulting system. Finally, we produce magnetic nanoparticles in the protein with gold clusters, achieving the coexistence of both nanostructures within a single protein molecule, a novel material unprecedented to date. We observed that factors such as temperature, metal/protein ratios, pH, dialysis, and purification processes all have an impact on protein recovery, loading efficiency, morphology, and nanoparticle size. Our findings highlight the development of ferritin-based nanomaterials as versatile platforms for potential biomedical use as multifunctional theranostic agents.","PeriodicalId":279,"journal":{"name":"Colloids and Surfaces B: Biointerfaces","volume":"245 ","pages":"114332"},"PeriodicalIF":5.4,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142563534","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Supramolecular nanofiber network hydrogel dressing for promoting wound healing with low swelling and mechanical stability properties. 用于促进伤口愈合的超分子纳米纤维网络水凝胶敷料，具有低肿胀和机械稳定性能。

IF 5.4 2区医学

Colloids and Surfaces B: Biointerfaces Pub Date : 2025-01-01 Epub Date: 2024-10-28 DOI: 10.1016/j.colsurfb.2024.114345

Si Qin, Huarun Li, Xiaochun Liu, Xinyao Zheng, Xiangyue Zhao, Shiyu Wen, Yeyang Wang, Ju Wen, Dawei Sun

{"title":"Supramolecular nanofiber network hydrogel dressing for promoting wound healing with low swelling and mechanical stability properties.","authors":"Si Qin, Huarun Li, Xiaochun Liu, Xinyao Zheng, Xiangyue Zhao, Shiyu Wen, Yeyang Wang, Ju Wen, Dawei Sun","doi":"10.1016/j.colsurfb.2024.114345","DOIUrl":"10.1016/j.colsurfb.2024.114345","url":null,"abstract":"Skin wounds are a major health problem of global concern. Prompt and proper care after skin injury is crucial for rapid healing and minimizing scar. Hydrogels are widely used wound dressings in clinical practice due to their ability to create a moist environment for wound healing. However, most hydrogels exhibit high swelling ratio and tend to compress and irritate the wound upon contact with wound exudate, which is counterproductive to the wound healing process. Supramolecular hydrogels formed by self-assembly of natural drug molecules have attracted increasing interest in wound healing due to their intrinsic pharmacological activity and excellent biocompatibility. In this study, a supramolecular nanofiber network hydrogel based on glycyrrhetinic acid (GA) was developed to promote wound healing. The hydrogel network consists of a self-assembled nanofibrous network generated by GA and a cross-linked network formed by gellan gum (GG). The resulting hydrogels have unique low swelling properties as well as good mechanical stability. What's more, the GG/GA hydrogels can absorb water and return to its original state after lyophilization, which facilitates storage. Both in vitro and in vivo studies demonstrated high biocompatibility and significant pro-angiogenic effects of GG/GA hydrogel. The wound healing ratio of the rat model treated with GG/GA hydrogel reached 95.49 ± 1.1 % at 14 days. These findings indicate that GG/GA supramolecular hydrogels possess significant potential in promoting wound healing and offer a novel approach for creating low-swelling, easy storage, inherently physiologically active, and highly biocompatibility wound dressings.","PeriodicalId":279,"journal":{"name":"Colloids and Surfaces B: Biointerfaces","volume":"245 ","pages":"114345"},"PeriodicalIF":5.4,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142581054","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Drug-induced self-assembled nanovesicles for chloroquine to sensitize MDR tumors to mitoxantrone hydrochloride. 药物诱导的氯喹自组装纳米囊，使 MDR 肿瘤对盐酸米托蒽醌敏感。

IF 5.4 2区医学

Colloids and Surfaces B: Biointerfaces Pub Date : 2025-01-01 Epub Date: 2024-11-04 DOI: 10.1016/j.colsurfb.2024.114358

Juan Wang, Xinchen Zhao, Liyan Qiu

{"title":"Drug-induced self-assembled nanovesicles for chloroquine to sensitize MDR tumors to mitoxantrone hydrochloride.","authors":"Juan Wang, Xinchen Zhao, Liyan Qiu","doi":"10.1016/j.colsurfb.2024.114358","DOIUrl":"10.1016/j.colsurfb.2024.114358","url":null,"abstract":"Multidrug resistance (MDR) is an incidental trouble post-chemotherapy, necessitating innovative therapeutic strategies. This study explores the potential of chloroquine (CQ) as a sensitizer for mitoxantrone hydrochloride (MitH) in drug-resistant tumors and introduces a novel pH-responsive drug-induced self-assembly nanovesicle (DIV) based on an amphiphilic polyphosphonitrile (PPAP) for the co-delivery of MitH and CQ. PPAP cannot self-assemble into nanovesicles alone, but when a certain amount of MitH was added, the multiple non-covalent interactions between PPAP and MitH contributed to the formation of DIV, which exactly improved the co-loading content of MitH and CQ to a large extent. CQ prevents MitH efflux and autophagy to reverse MitH resistance. Given the synergy between MitH and CQ at a 1:2 mass ratio with a combination index of 0.40 in K562/ADR cells, MitH and CQ co-loaded DIV (MC-DIV) is constructed and demonstrates a sensitivity index of 7.1 on cytotoxicity compared to free MitH. Furthermore, MC-DIV achieves extended circulation time, synchronous dual-drug delivery, and improved tumor targeting following systemic administration, resulting in exceptional antitumor efficacy in K562/ADR xenograft models with a tumor inhibition rate of 83.0 %. Overall, MC-DIV provides a viable method to maximize the loading capacity of nanocarriers, and potentially serves as a promising formulation for various MitH-resistant tumors.","PeriodicalId":279,"journal":{"name":"Colloids and Surfaces B: Biointerfaces","volume":"245 ","pages":"114358"},"PeriodicalIF":5.4,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142602367","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Can polymeric nanofibers effectively preserve and deliver live therapeutic bacteria? 聚合物纳米纤维能否有效保存和输送活的治疗性细菌？

IF 5.4 2区医学

Colloids and Surfaces B: Biointerfaces Pub Date : 2025-01-01 Epub Date: 2024-10-21 DOI: 10.1016/j.colsurfb.2024.114329

Nina Katarina Grilc, Julijana Kristl, Špela Zupančič

{"title":"Can polymeric nanofibers effectively preserve and deliver live therapeutic bacteria?","authors":"Nina Katarina Grilc, Julijana Kristl, Špela Zupančič","doi":"10.1016/j.colsurfb.2024.114329","DOIUrl":"10.1016/j.colsurfb.2024.114329","url":null,"abstract":"Probiotics and live therapeutic bacteria (LTB), their strictly regulated therapeutic counterpart, are increasingly important in treating and preventing biofilm-related diseases. This necessitates new approaches to (i) preserve bacterial viability during manufacturing and storage and (ii) incorporate LTB into delivery systems for enhanced therapeutic efficacy. This review explores advances in probiotic and LTB product development, focusing on preservation, protection, and improved delivery. Preservation of bacteria can be achieved by drying methods that decelerate metabolism. These methods introduce stresses affecting viability which can be mitigated with suitable excipients like polymeric or low molecular weight stabilizers. The review emphasizes the incorporation of LTB into polymer-based nanofibers via electrospinning, enabling simultaneous drying, encapsulation, and delivery system production. Optimization of bacterial survival during electrospinning and storage is discussed, as well as controlled LTB release achievable through formulation design using gel-forming, gastroprotective, mucoadhesive, and pH-responsive polymers. Evaluation of the presence of the actual therapeutic strains, bacterial viability and activity by CFU enumeration or alternative analytical techniques is presented as a key aspect of developing effective and safe formulations with LTB. This review offers insights into designing delivery systems, especially polymeric nanofibers, for preservation and delivery of LTB, guiding readers in developing innovative biotherapeutic delivery systems.","PeriodicalId":279,"journal":{"name":"Colloids and Surfaces B: Biointerfaces","volume":"245 ","pages":"114329"},"PeriodicalIF":5.4,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142563522","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Advances in the transport of oral nanoparticles in gastrointestinal tract. 口服纳米颗粒在胃肠道中的转运研究进展。

IF 5.4 2区医学

Colloids and Surfaces B: Biointerfaces Pub Date : 2025-01-01 Epub Date: 2024-10-15 DOI: 10.1016/j.colsurfb.2024.114321

Tingting Song, Lu Yuan, Jie Wang, Wenjing Li, Yong Sun

引用次数: 0

Construction of glycosylated zein-based colloids to simultaneously improve fucoxanthin's thermal processing adaptability, digestive stability, and oral bioavailability. 构建糖基化玉米蛋白胶体，以同时改善岩藻黄质的热加工适应性、消化稳定性和口服生物利用度。

IF 5.4 2区医学

Colloids and Surfaces B: Biointerfaces Pub Date : 2025-01-01 Epub Date: 2024-10-23 DOI: 10.1016/j.colsurfb.2024.114334

Yuanjie Guo, Hailan Wang, Liyuan Ma, Zixin Guo, Yixiang Liu, Jie Zheng

{"title":"Construction of glycosylated zein-based colloids to simultaneously improve fucoxanthin's thermal processing adaptability, digestive stability, and oral bioavailability.","authors":"Yuanjie Guo, Hailan Wang, Liyuan Ma, Zixin Guo, Yixiang Liu, Jie Zheng","doi":"10.1016/j.colsurfb.2024.114334","DOIUrl":"10.1016/j.colsurfb.2024.114334","url":null,"abstract":"Fucoxanthin (FX) is a carotenoid found in marine environments with a range of nutritional functions. However, its application in the food industry has been restricted by its vulnerability to deterioration and absorption challenges. This study employed zein to develop hydrophilic colloids to enhance the thermal processing adaptability, gastrointestinal digestive stability, and oral bioavailability of FX. The findings demonstrated that the using glucose for the grafting modification of zein caused a deviation in its isoelectric point, reduced its water contact angle, and altered its secondary structure, resulting in higher hydrophilicity. Using glycosylated zein (GZ) for FX loading yielded homogenous, stable aqueous GZ-FX complex dispersion solutions with an encapsulation efficiency (EE) > 85.00 %, a particle size < 210.00 nm, a zeta-potential > -30.00 mV, and a polydispersity index (PDI) < 0.30. GZ-based encapsulation notably enhanced the thermal stability of FX, retaining approximately 90.00 % and 80.00 % of the FX at 65 ℃ and 100 ℃, respectively. During in vitro simulated gastrointestinal digestion, GZ-encapsulation of FX demonstrated a retention increase of 30.63 % and a 2.31-fold higher micellization rate. The in vivo absorption results showed that GZ-based encapsulation dramatically increased FX oral bioavailability, while its serum, liver, and kidney response levels were 51.49-fold, 5.13-fold and 6.73-fold higher. This study suggests that glycosylated alcohol-soluble proteins are highly effective carriers for delivering carotenoids, with significant application potential in the food industry.","PeriodicalId":279,"journal":{"name":"Colloids and Surfaces B: Biointerfaces","volume":"245 ","pages":"114334"},"PeriodicalIF":5.4,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142542494","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Co-delivery of SN38 and MEF2D-siRNA via tLyp-1-modified liposomes reverses PD-L1 expression induced by STING activation in hepatocellular carcinoma. 通过tLyp-1修饰的脂质体联合递送SN38和MEF2D-siRNA可逆转STING激活诱导的肝细胞癌PD-L1表达。

IF 5.4 2区医学

Colloids and Surfaces B: Biointerfaces Pub Date : 2025-01-01 Epub Date: 2024-10-13 DOI: 10.1016/j.colsurfb.2024.114318

Jiawei Du, Ziting Que, Ailifeire Aihaiti, Mengyan Zhai, Zhiwei Zhang, Yong Shao, Ying Zhang, Fengqin Miao, Yuqing Shen, Xin Chen, Jianqiong Zhang

{"title":"Co-delivery of SN38 and MEF2D-siRNA via tLyp-1-modified liposomes reverses PD-L1 expression induced by STING activation in hepatocellular carcinoma.","authors":"Jiawei Du, Ziting Que, Ailifeire Aihaiti, Mengyan Zhai, Zhiwei Zhang, Yong Shao, Ying Zhang, Fengqin Miao, Yuqing Shen, Xin Chen, Jianqiong Zhang","doi":"10.1016/j.colsurfb.2024.114318","DOIUrl":"10.1016/j.colsurfb.2024.114318","url":null,"abstract":"Hepatocellular carcinoma (HCC) exhibits an immunosuppressive tumor microenvironment, leading to a low objective response rate when immune checkpoint inhibitors (ICIs) are utilized. The cGAS-STING pathway demonstrates a powerful immune stimulatory effect, nevertheless, activation of this pathway triggers an upregulation of PD-L1, which inhibits the anti-tumor function of immune cells. The present study discovered that knockdown of MEF2D by a siRNA in H22 cells decreases the expression of PD-L1. Subsequently, tLyp-1-modified liposomes were developed for the delivery of SN38 and MEF2D-siRNA. The outcomes indicated that the modification of tLyp-1 could enhance the uptake of liposomes by tumor cells. tLip/siMEF2D/SN38 liposomes can effectively knockdown the expression of MEF2D in HCC cells and reduce the expression of PD-L1 in vitro and in vivo, thereby enhancing proliferation inhibition and apoptosis induction, and effectively suppressing the growth of tumors. SN38 treatment elevated the expression of p-TBK1 and p-IRF3 in tumor tissue, signifying the activation of the cGAS-STING pathway and facilitating the maturation of dendritic cells in vitro and in vivo. At the same time, the co-delivery of MEF2D-siRNA reduced the expression of PD-L1, thereby decreasing the quantity of M2 macrophages and myeloid-derived suppressor cells (MDSCs) in tumors, increasing the number of CD4+ T cells within the tumor, and strengthening the anti-tumor immune efficacy. In conclusion, our results suggest that tLyP-1 modified, SN38- and MEF2D siRNA-loaded liposomes have the potential for the treatment of HCC and optimize the immunotherapy of HCC via STING activation.","PeriodicalId":279,"journal":{"name":"Colloids and Surfaces B: Biointerfaces","volume":"245 ","pages":"114318"},"PeriodicalIF":5.4,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142454502","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0