Colloids and Surfaces B: Biointerfaces最新文献

{"title":"Revisiting the characteristics of nanomaterials, composites, hybrid and functionalized materials in medical microbiology","authors":"Jennifer Mariana Vargas López ,&nbsp;José Alfonso Cruz Ramos ,&nbsp;Gregorio Guadalupe Carbajal Arizaga","doi":"10.1016/j.colsurfb.2025.114556","DOIUrl":"10.1016/j.colsurfb.2025.114556","url":null,"abstract":"<div><div>Unlike traditional materials designed to form large structures, many modern materials are presented in the form of powders resulting from a molecular level control of their composition and structure, making possible the miniaturization and fine-tuning of their properties to act in cellular dimensions with customized tasks. Several new materials for biomedical and microbiology applications appear every year. Although many of them are called nanomaterials, there may be a more precise description or classification. In this work, we review and detail the structural classification of nanometric, functionalized, hybrid and composite materials, mainly based on descriptions given by the International Union of Pure and Applied Chemistry (IUPAC). Besides we included smart and multifunctional materials, cassification based on performance. The second section shows how these materials are used in the area of medical microbiology, grouping these applications into barriers for microorganisms on surfaces, disinfectants in clinical practice, targeting of pathogens, detectors of microorganisms or their metabolites, and also as substrates to stabilize, transport, or nourish beneficial microorganisms. Finally, we will discuss some evidence that indicates the environmental risk and bacterial resistance alerts that should be taken into account with the use of these advanced powder materials.</div></div>","PeriodicalId":279,"journal":{"name":"Colloids and Surfaces B: Biointerfaces","volume":"250 ","pages":"Article 114556"},"PeriodicalIF":5.4,"publicationDate":"2025-02-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143419145","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Proteomic evaluation of borosilicate hybrid sol-gel coatings with osteogenic, immunomodulatory and antibacterial properties","authors":"Francisco Romero-Gavilán ,&nbsp;Andreia Cerqueira ,&nbsp;Iñaki García-Arnáez ,&nbsp;Loredana Scalschi ,&nbsp;Begonya Vicedo ,&nbsp;Mikel Azkargorta ,&nbsp;Félix Elortza ,&nbsp;Raúl Izquierdo ,&nbsp;Mariló Gurruchaga ,&nbsp;Isabel Goñi ,&nbsp;Julio Suay","doi":"10.1016/j.colsurfb.2025.114561","DOIUrl":"10.1016/j.colsurfb.2025.114561","url":null,"abstract":"<div><div>Silica hybrid sol-gel coatings represent an interesting approach to bioactivate dental implants. Boron is known for its osteogenic, angiogenic and antibacterial functions in biomedical applications. This study describes the synthesis of a novel borosilicate hybrid sol-gel coating using a mixture of methyltrimethoxysilane, tetraethyl orthosilicate and trimethyl borate (TMB). Coatings with different amounts of boron were obtained, and their physiochemical properties were examined; <em>in vitro</em> tests with human osteoblasts and macrophages (THP-1) were carried out. The effects of these materials on bacteria viability were evaluated using <em>Escherichia coli</em> and <em>Staphylococcus aureus</em>. The human serum proteins adsorbed onto the coatings were analysed employing proteomic techniques. To synthesise the new materials, the appropriate sol-gel reactions were developed; boron was integrated into the silica network, and well-adhering coatings were obtained. These borosilicate coatings were non-cytotoxic, displayed osteogenic potential, and upregulated adsorption of proteins related to bone regeneration (IGF2, ALS and APOE). Boron upregulated the expression of TNF-α, INFg and TGF-β and increased the TNF-α and TGF-β cytokine production in THP-1. Moreover, the addition of boron caused downregulation of NOX2 expression. Proteomic analysis revealed that boron-doping reduced the adsorption of immunoglobulins and complement system proteins. It also caused an increase in the levels of apolipoproteins, antioxidant proteins and serum amyloid A proteins, which was in agreement with <em>in vitro</em> results. The coatings with 10 and 20 % TMB displayed antibacterial effect against <em>S. aureus</em>. The results of this study will enhance our comprehension of interactions between boron-containing biomaterials and biological systems.</div></div>","PeriodicalId":279,"journal":{"name":"Colloids and Surfaces B: Biointerfaces","volume":"250 ","pages":"Article 114561"},"PeriodicalIF":5.4,"publicationDate":"2025-02-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143419225","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Cobalt carbonate nanorods enhance chemotherapy via neutralization of acidic tumor microenvironment and generation of carbonate radical anions for necrosis","authors":"Min Wu ,&nbsp;Bing Liang ,&nbsp;Lu Zhang ,&nbsp;Benmeng Wu ,&nbsp;Jingjing Liu","doi":"10.1016/j.colsurfb.2025.114563","DOIUrl":"10.1016/j.colsurfb.2025.114563","url":null,"abstract":"<div><div>One of the hallmarks of cancer is the acidic extracellular space surrounding the tumor, which is linked to metabolic reprogramming and the use of glycolysis. Additionally, the acidic tumor microenvironment (TME) establishes a physiological barrier called \"ion trapping\" and significantly lowers the ability of cells to absorb weak-base chemotherapy agents. Although CO₃^2- containing agents and nanoformulations could effectively neutralize the tumor acidity, the CO₃^2- based therapeutic effect was insufficiently investigated. Herein, we fabricated cobalt carbonate (CoCO₃) nanorods as drug carriers with acidity-responsive dissociation and acidity neutralization properties for the loading of hydrophobic and weak-basic drugs, evodiamine (EVO). After effective surface modification, CoCO₃-PEG-EVO could effectively accumulate in the tumor and inhibit the growth of the tumor. On the one hand, acidity neutralization of CoCO₃-PEG-EVO could lead to the ion trapping overcome and cellular uptake of EVO enhancement for effective cancer cell apoptosis. On the other hand, the high level of H₂O₂ in the tumor and HCO₃^- from dissociated CoCO₃-PEG-EVO could cause the generation of CO₃·^- through a Fenton-like reaction while not hydroxyl radical (·OH) for cancer cell necrosis. Our results thus point to a potent yet easily prepared CoCO₃ nanosystem (CoCO₃-PEG-EVO) to induce cancer cell death, and because of its well-defined composition and excellent biocompatibility, it may be used in clinical settings in the future.</div></div>","PeriodicalId":279,"journal":{"name":"Colloids and Surfaces B: Biointerfaces","volume":"250 ","pages":"Article 114563"},"PeriodicalIF":5.4,"publicationDate":"2025-02-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143444473","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Carbon nanoparticles-Fe(II) complex combined with sorafenib for ferroptosis-induced antitumor effects in triple-negative breast cancer","authors":"Ping Xie ,&nbsp;Ting Qu ,&nbsp;Kexin Tang ,&nbsp;Yuanfang Huang ,&nbsp;Guangfu Zeng ,&nbsp;Huahui Yuan ,&nbsp;Qian Xin ,&nbsp;Yufeng Zhao ,&nbsp;Jinmei Yang ,&nbsp;Cheng Zeng ,&nbsp;Xian Wu ,&nbsp;Sheng-Tao Yang ,&nbsp;Xiaohai Tang","doi":"10.1016/j.colsurfb.2025.114562","DOIUrl":"10.1016/j.colsurfb.2025.114562","url":null,"abstract":"<div><div>Triple negative breast cancer (TNBC) represents an aggressive subtype of breast cancer that lacks the expression of estrogen receptor, progesterone receptor, and human epidermal growth factor receptor 2, whose systemic treatment options are currently limited to chemotherapy. Carbon nanoparticles-Fe(II) complex (CNSI-Fe) is a promising antitumor drug that induces ferroptosis to kill tumor cells efficiently. In this study, we combined CNSI-Fe and a ferroptosis inducer sorafenib (SRF) to achieve the efficient chemotherapy of TNBC. CNSI-Fe could adsorb SRF by hydrophobic interaction and π-π stacking with a maximum adsorption capacity of 31 mg/g. During the in vitro assays, CNSI-Fe+SRF combination inhibited the cell viability of 4T1 cells much more efficiently than CNSI-Fe or SRF alone. The high Fe uptake, hydroxyl radical generation and oxidative damages verified the ferroptosis of 4T1 cells upon the CNSI-Fe+SRF treatment. During the in vivo evaluations, SRF enhanced the therapeutic effect of CNSI-Fe as indicated by the higher tumor growth inhibition rate of 67.8 % and the higher survival rate. CNSI captured SRF in tumor to give a 6 mg/kg uptake, which lowered the glutathione peroxidase 4 (GPX4) level and enhanced the hydroxyl radical production of 4T1 tumor. In addition, CNSI-Fe treatment up-regulated the genes associated with antioxidative responses, but the up-regulation was offset by SRF. CNSI-Fe+SRF group showed similar toxicity to mice as SRF alone in the biosafety evaluations. Our results collectively indicated that the combination of CNSI-Fe and SRF could efficiently treat TNBC through ferroptosis.</div></div>","PeriodicalId":279,"journal":{"name":"Colloids and Surfaces B: Biointerfaces","volume":"250 ","pages":"Article 114562"},"PeriodicalIF":5.4,"publicationDate":"2025-02-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143419146","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Antibacterial and bactericidal properties of resin nanostructures coated with SiO2 thin films","authors":"Yuito Matsushita ,&nbsp;Gakuto Inoue ,&nbsp;Zihao Zhao ,&nbsp;Natsuki Ogawa ,&nbsp;Hitoshi Ishiguro ,&nbsp;Kayano Sunada ,&nbsp;Kenta Ishibashi ,&nbsp;Hiroaki Kojima ,&nbsp;Tomohiro Shimizu ,&nbsp;Shoso Shingubara ,&nbsp;Takeshi Ito","doi":"10.1016/j.colsurfb.2025.114560","DOIUrl":"10.1016/j.colsurfb.2025.114560","url":null,"abstract":"<div><div>Nanotextures exhibit physical antibacterial and bactericidal properties; hence, they have great potential to prevent infection related to bacteria through contact. The surface characteristics of a nanostructure determine its antibacterial and bactericidal activities. In this study, an atomic layer deposition (ALD) was used to prepare nano-level, hard, thin SiO₂ layers on resin nanostructures and their effects were demonstrated through antibacterial and bactericidal tests. The SiO₂-layer-coated resin nanostructure exhibited a water contact angle of 7.2°, dramatically lower than that of the uncoated specimen (130.2°), as well as a three-fold higher local elastic modulus. Further, 10-nm-thin SiO₂-layer-coated nanopillars showed antibacterial and bactericidal effects against <em>E. coli</em>. These results demonstrate that SiO₂ thin layer coating has great potential for improving the antibacterial and bactericidal properties of polymeric nanopillar arrays.</div></div>","PeriodicalId":279,"journal":{"name":"Colloids and Surfaces B: Biointerfaces","volume":"250 ","pages":"Article 114560"},"PeriodicalIF":5.4,"publicationDate":"2025-02-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143419147","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A computer-aided, carrier-free drug delivery system with enhanced cytotoxicity and biocompatibility: A universal model for multifunctional lung cancer therapy","authors":"Zhonglei Wang ,&nbsp;Wenjing Xu ,&nbsp;Shizeng Lei ,&nbsp;Yuxin Lai ,&nbsp;Yingying Zhang ,&nbsp;Ying Wang ,&nbsp;Ziling Xiang ,&nbsp;Xin Fu ,&nbsp;Liyan Yang","doi":"10.1016/j.colsurfb.2025.114557","DOIUrl":"10.1016/j.colsurfb.2025.114557","url":null,"abstract":"<div><div>Erlotinib (ERL) is a first-line targeted therapy for patients with epidermal growth factor receptor (EGFR)-mutant advanced non-small cell lung cancer (NSCLC). However, its effectiveness is hindered by acquired resistance and poor bioavailability. Carrier-free nanodrugs are a research hotspot due to their efficient targeting, high drug loading capacity, and the absence of any excipients. Herein, we report an advanced self-delivery system for multimodal NSCLC therapy using a computer-aided strategy. First, we developed a novel heterodimer, ERL-SS-QM (ERL conjugated with QM-OH—a hydrophobic aggregation-induced emission fluorophore—via a disulfide bond [SS]), which serves as both cargo and carrier material. Self-assembly is driven by multiple noncovalent interactions, including π-π stacking and sulfur bonds. Subsequently, an ERL-SS-QM-based \"triadic\" drug delivery nanoplatform comprising 21 variants was developed. A case study on ursolic acid (UA)-loaded ERL-SS-QM nanoparticles (named UA@ERL-SS-QM NPs) revealed narrow size distribution, small particle size, and well stabilized (zeta potential = −28.9 mV). The UA@ERL-SS-QM NPs demonstrated concentration-dependent toxicity against targeted A549 cells (IC₅₀ = 4.36 μM), outperforming free monomeric drugs ERL (IC₅₀ = 12.94 μM) and UA (IC₅₀ = 12.21 μM), indicating good efficiency. Conversely, these NPs exhibited minimal cytotoxicity in non-targeted BEAS-2B cells, suggesting favorable biocompatibility. Upon endocytosis and interaction with overexpressed GSH in A549 cells, the disulfide-bond linker is cleaved to release three components: ERL, UA (which downregulates β-catenin/TCF4/CT45A2 signaling pathways, inducing apoptosis in ERL-resistant L858R/T790M mutant cells—a key factor in acquired resistance to ERL treatment), and QM-OH. Hence, this work provides a universal model for multifunctional NSCLC therapy that effectively addresses ERL resistance while enhancing cytotoxicity and biocompatibility.</div></div>","PeriodicalId":279,"journal":{"name":"Colloids and Surfaces B: Biointerfaces","volume":"250 ","pages":"Article 114557"},"PeriodicalIF":5.4,"publicationDate":"2025-02-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143377214","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Human amniotic mesenchymal stem cells improve patency and regeneration of electrospun biodegradable vascular grafts via anti-thrombogenicity and M2 macrophage polarization","authors":"Nuoxin Wang ,&nbsp;Haoyuan Wang ,&nbsp;Dong Weng ,&nbsp;Bin Wang ,&nbsp;Juan Wang ,&nbsp;Jing He ,&nbsp;Xiumei Mo ,&nbsp;Feng Wang ,&nbsp;Zhixu He","doi":"10.1016/j.colsurfb.2025.114559","DOIUrl":"10.1016/j.colsurfb.2025.114559","url":null,"abstract":"<div><div>Small-diameter vascular grafts (SDVGs) are prone to thrombosis and have low long-term patency rates for various reasons, which cannot meet the clinical requirements. In this work, Human amniotic mesenchymal stem cell (hAMSC) seeding electrospun polylactic acid-co-polycaprolactone (PLCL) SDVGs are fabricated and their application potential is systematically evaluated. The SDVG has excellent mechanical properties. PLCL eletrospinning membrane has no cytotoxicity. The SDVG has a porous fibrous tube wall, uniform distribution of hAMSCs, and good cell compatibility, blood compatibility, histocompatibility and mechanical properties. hAMSCs loading can improve the acute antithrombotic ability, patency and in vivo regeneration effect of PLCL electrospun SDVGs. The mechanism is related to hAMSCs increasing the content of endothelial cells, contractile smooth muscle cells, and M2 macrophages, as well as activating extracellular matrix production.</div></div>","PeriodicalId":279,"journal":{"name":"Colloids and Surfaces B: Biointerfaces","volume":"250 ","pages":"Article 114559"},"PeriodicalIF":5.4,"publicationDate":"2025-02-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143402876","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Free-standing, flexible and conformable bilayered polymeric nanomembranes modified with gold nanomaterials as electronic skin sensors","authors":"Samuele Colombi ,&nbsp;Carlos Alemán ,&nbsp;Jose García-Torres","doi":"10.1016/j.colsurfb.2025.114558","DOIUrl":"10.1016/j.colsurfb.2025.114558","url":null,"abstract":"<div><div>Skin is a barrier that protects us against physical, chemical and biological agents. However, any damage to the skin can disrupt this barrier and therefore compromise its function leading to sometimes catastrophic consequences like sepsis. Thus, methods to detect early signs of infection are necessary. In this work, we have developed a straightforward method for producing 2D nanomembranes with regularly spaced 1D metallic nanostructures integrating sensing capabilities to pH and NADH (nicotinamide adenine dinucleotide), which are critical analytes revealing infection. To achieve this, we have successfully fabricated a bilayered nanomembrane combining a pH-responsive polyaniline (PANI) layer and a nanoperforated poly(lactic acid) (PLA) layer containing gold nanowires (Au NWs) as NADH sensing element. SEM, FTIR, Raman and AFM techniques revealed the formation of the bilayered PANI/PLA nanomembrane and the successful incorporation of the Au NWs inside the nanoperforations. The resulting bilayered nanomembrane showed significant flexibility and conformability onto different substrates due to the softness of the polymers and the ultrathin thickness with stiffness values similar to human skin. These nanomembranes also exhibited remarkable electrochemical sensing performance towards pH and NADH detection. Thus, the nanomembrane displayed linearity with good sensitivity (47 mV pH⁻¹) in the critical pH range 4–10 and fast response time (10 s). On the other hand, PANI/PLA-Au nanomembranes also allowed the quantitative sensing of NADH with a limit of detection of 0.39 mM and a sensitivity of 1 µA cm⁻² mM⁻¹ in the concentration range 0–5 mM.</div></div>","PeriodicalId":279,"journal":{"name":"Colloids and Surfaces B: Biointerfaces","volume":"250 ","pages":"Article 114558"},"PeriodicalIF":5.4,"publicationDate":"2025-02-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143395539","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Antitumour and anti-angiogenesis efficacy of a multifunctional self-oxygenated active-targeting drug delivery system by encapsulating biological and chemotherapeutic drugs","authors":"Ming Zhu ,&nbsp;Yizhuo Xie ,&nbsp;Zhiping Li ,&nbsp;Han Bao ,&nbsp;Dongfanghui Miao ,&nbsp;Xin Guo ,&nbsp;Shanshan Wang ,&nbsp;Kejia Chen ,&nbsp;Hongzhu Chen ,&nbsp;Jingwen Dai ,&nbsp;Na Yang ,&nbsp;Liangping Yu ,&nbsp;Jin Pei","doi":"10.1016/j.colsurfb.2025.114549","DOIUrl":"10.1016/j.colsurfb.2025.114549","url":null,"abstract":"<div><div>The hypoxic tumour microenvironment (TME), resulting from abnormal tumour angiogenesis, is a major factor contributing to treatment failure in breast cancer patients. In this study, we present a ZnO₂-based oestrone-conjugated PEGylated liposome (ZnO₂@EPL-CDDP/EGCG) that incorporates cisplatin (CDDP) and epigallocatechin-3-gallate (EGCG). ZnO₂ remains stable in neutral environments but decomposes under mildly acidic conditions, releasing Zn²⁺ and H₂O₂. These byproducts inhibit the electron transport chain, stimulate the endogenous reactive oxygen species production for chemodynamic therapy (CDT), and generate oxygen at tumour sites to alleviate hypoxia and enhance anti-angiogenic efficacy. EGCG inhibits tumour angiogenesis by down-regulating hypoxia-inducible factor-1α (HIF-1α) and its downstream pathways, while also exhibiting synergistic anti-tumour effects with CDDP. Oestrone-conjugated and polyethylene glycol (PEG) modifications facilitate targeted accumulation at tumour sites. Our findings indicate that ZnO₂@EPL-CDDP/EGCG significantly improves the therapeutic efficacy of both EGCG and CDDP, remodels tumour vasculature, and alleviates hypoxia within the TME. This self-oxygenated, actively targeted drug delivery system notably extends the survival of healthy ICR mice without observed toxicity. This novel approach, which co-encapsulates ZnO₂, EGCG, and CDDP in an active-targeting liposomal formulation for the first time, represents a promising strategy for effective cancer treatment.</div></div>","PeriodicalId":279,"journal":{"name":"Colloids and Surfaces B: Biointerfaces","volume":"250 ","pages":"Article 114549"},"PeriodicalIF":5.4,"publicationDate":"2025-02-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143429618","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Recent advances of physiochemical cues on surfaces for directing cell fates","authors":"Wenwen Deng ,&nbsp;Xiufen Yang ,&nbsp;Jiangnan Yu ,&nbsp;Emmanuel Omari-Siaw ,&nbsp;Ximing Xu","doi":"10.1016/j.colsurfb.2025.114550","DOIUrl":"10.1016/j.colsurfb.2025.114550","url":null,"abstract":"<div><div>Surface modification plays an essential role in dictating cell behavior and fate, as it creates a microenvironment that profoundly influences cell attachment, migration, proliferation, and differentiation. This review aims to the intricate interplay of culture surface properties, including topography, stiffness, charge, and chemical modifications, demonstrating their profound impact on cell destiny. We explore the nuanced responses of cells to varying surface topographies, from nano- to microscale features, elucidating the influence of geometric patterns and roughness. We also investigate the impact of substrate stiffness, highlighting the way cells perceive and respond to mechanical cues mimicking their native environments. The role of surface charge is examined, revealing how electrostatic interactions influence cell adhesion, signaling, and cell fate decisions. Finally, we delve into the diverse effects of chemical modifications, including the presentation of bioactive molecules, growth factors, and extracellular matrix (ECM) components, demonstrating their ability to guide cell behavior and stimulate specific cellular responses. This review offers comprehensive insights into the important role of surface properties in shaping cell fate, offering promising avenues for developing sophisticated cell culture platforms for applications in drug discovery, regenerative medicine, and fundamental research.</div></div>","PeriodicalId":279,"journal":{"name":"Colloids and Surfaces B: Biointerfaces","volume":"250 ","pages":"Article 114550"},"PeriodicalIF":5.4,"publicationDate":"2025-02-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143372599","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}