Colloids and Surfaces B: Biointerfaces最新文献

{"title":"Exploiting aza-zinc-dipyrromethene dyes for photoacoustic imaging guided synergistic anticancer phototherapy","authors":"Ruyi Zhou ,&nbsp;Hongxia Tian ,&nbsp;Yu Xue ,&nbsp;Wen Lv ,&nbsp;Mingye Zhu ,&nbsp;Jiangang Li ,&nbsp;Shujuan Liu ,&nbsp;Qiang Zhao","doi":"10.1016/j.colsurfb.2025.115076","DOIUrl":"10.1016/j.colsurfb.2025.115076","url":null,"abstract":"<div><div>Developing an innovative phototherapy agent that integrates tumor diagnosis and treatment functions is crucial for improving the precision and efficacy of anti-tumor treatment. Traditional aza-dipyrromethene (aza-DP) dyes exhibited strong photothermal effects, high molar absorption coefficients, and good photostability, but their photodynamic effects were weak. Metal elements were introduced into the aza-DP structure to address this issue, thereby enhancing inter-system crossing (ISC) and improving the photodynamic effect. In this study, a series of Zn(II)-chelated aza-DP complexes were designed and synthesized as novel phototherapy agents. To further extend the absorption wavelength of aza-DP in the near-infrared region, strong electron-donating groups of N,N-dimethylaniline were introduced at the 1,7 positions for modification, and both photodynamic and photothermal effects were also improved. The nanoparticles prepared by coating the phototherapy agents as mentioned above with amphiphilic DSPE-mPEG₅₀₀₀ exhibit excellent hydrophilicity and photostability. Under 660 nm laser irradiation, the nanoparticles exhibited good synergistic therapeutic effects <em>in vivo</em>, with significant anti-tumor effects and minimal accompanying side effects. Meanwhile, considering its excellent photothermal properties, photoacoustic imaging technology was utilized to provide comprehensive guidance for the precise localization of tumors in mice. The study shows that rational modification of traditional aza-DP-based synergistic phototherapy agents can yield unexpected enhancements in their therapeutic effects, providing new ideas for anti-tumor drug development.</div></div>","PeriodicalId":279,"journal":{"name":"Colloids and Surfaces B: Biointerfaces","volume":"256 ","pages":"Article 115076"},"PeriodicalIF":5.6,"publicationDate":"2025-08-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144913898","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Scalable synthesis of self-assembled magneto-plasmonic core-satellite nanoparticles for microfluidic sorting and bioorthogonal sensing of targeted cells","authors":"F. Mattii ,&nbsp;G. Feregotto ,&nbsp;B. Muzzi ,&nbsp;F.S. Pavone ,&nbsp;M. Calamai ,&nbsp;C. Capitini ,&nbsp;C. Dallari ,&nbsp;C. Credi","doi":"10.1016/j.colsurfb.2025.115040","DOIUrl":"10.1016/j.colsurfb.2025.115040","url":null,"abstract":"<div><div>Multifunctional magneto-plasmonic nanoparticles (MP-NPs) are attracting increasing interest for biomedical applications due to their dual magnetic and optical properties. However, existing synthesis protocols for MP-NPs could be limited by harsh conditions or lengthy, complex procedures. These limitations can hinder the development of nanosystems that work effectively in biological dispersion. In this work, we present a flexible nanoplatform for capture and probe applications, based on MP-NPs and bioorthogonal plasmonic nanoparticles (AuNPs). This system enables efficient cell sorting and surface-enhanced Raman scattering (SERS) detection in complex biological media under dynamic conditions. Magnetic Fe₃O₄ nanoclusters were synthesized using a scalable, aqueous Massart-based protocol. These structures were then densely decorated with electrostatically assembled AuNPs, producing magnetically responsive and plasmonically active nanocomposites with high aqueous stability. A polyethylene glycol (PEG) derivative was used for surface functionalization, improving colloidal stability and enabling broad bio-recognition capability. To enhance detection sensitivity and shift the optical response into the near-infrared (NIR) range, bioorthogonal Raman-active gold nanostars (AuNSts) were integrated into a sandwich configuration with the MP-NPs. The system demonstrated selective sorting and sensitive SERS-based identification of cell subpopulation within a heterogeneous sample. Additionally, the integration of the MP-NPs assay into a 3D-printed microfluidic device allowed controlled nanoparticles manipulation using external magnets, enhancing magnetic separation, hot-spots generation, and assay reusability. Overall, these MP-NPs platform combines scalable synthesis, tunable colloidal and surface properties, and compatibility with microfluidic systems. This work lays the foundation for advanced biosensing approaches in lab-on-chip formats for clinical diagnostics and targeted analysis of rare cell populations.</div></div>","PeriodicalId":279,"journal":{"name":"Colloids and Surfaces B: Biointerfaces","volume":"256 ","pages":"Article 115040"},"PeriodicalIF":5.6,"publicationDate":"2025-08-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144916960","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Multi functional carbon dots for precision cancer treatment: Synthesis regulation, performance enhancement, and future applications","authors":"Shanshan Liu ,&nbsp;Yimin Guo ,&nbsp;Yun Ren ,&nbsp;Fubao Han ,&nbsp;Tao Zhang","doi":"10.1016/j.colsurfb.2025.115068","DOIUrl":"10.1016/j.colsurfb.2025.115068","url":null,"abstract":"<div><div>Carbon dots (CDs) are an emerging kind of carbon nanomaterials with characteristic sizes less than 10 nm, graphitized carbon nuclei and a rich variety of surface functional groups. Due to its simple and rapid synthesis, unique electronic structure, excellent photoluminescence properties, good biocompatibility, and environmental friendliness, CDs have been widely used in fields <em>i.e.</em> antibacterial, analytical detection, biological imaging, drug delivery, and controlling environmental pollutants. Especially in the emerging field of biomedical anti-tumor therapy, its application is becoming increasingly widespread, mainly based on the stimulation of CDs in specific chemical environments, <em>i.e.</em>, light and ultrasound can increase intracellular reactive oxygen species (ROS) levels, disrupt intracellular redox balance, and achieve the effect of killing tumors. Therefore, it has attracted the attention of researchers around the world. However, there is currently a lack of in-depth analysis on the application of CDs in biological anti-tumor, and the understanding of the development and new breakthroughs of CDs is relatively limited. Therefore, it is necessary to conduct a timely and comprehensive review of the widely dispersed literature on their application in biological anti-tumor, to stimulate ideas that can lead to more innovative practical applications. The scope of our review is significantly different from other existing review papers in similar fields. It focuses on the potential correlation between its synthesis and surface structure with its specific applications, and analyzes the fundamental reasons for its application in emerging therapies such as photothermal therapy, photodynamic therapy, and sonodynamic therapy (As shown in Figure 1). Through an overall review of the latest research results, a detailed summary and discussion will be conducted on the bottlenecks and opportunities of CDs in the application of cancer treatment in the future.</div></div>","PeriodicalId":279,"journal":{"name":"Colloids and Surfaces B: Biointerfaces","volume":"256 ","pages":"Article 115068"},"PeriodicalIF":5.6,"publicationDate":"2025-08-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144913896","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Porous microneedle-mediated continuous glucose monitoring based on reverse iontophoresis","authors":"Rui Yao ,&nbsp;Olanrewaju Yaasir Olatunji ,&nbsp;Rui Wang ,&nbsp;Zhenyu Wu ,&nbsp;Guohua Jiang","doi":"10.1016/j.colsurfb.2025.115078","DOIUrl":"10.1016/j.colsurfb.2025.115078","url":null,"abstract":"<div><div>Measuring blood glucose concentrations via continuous glucose monitoring is an important aspect of management for patients with diabetes. In this study, a continuous glucose monitoring device has been developed by integration of porous microneedles, reverse iontophoresis and biosensor technologies. An enzyme-based glucose biosensor as working electrode was firstly fabricated by electrodepositing polydopamine (PDA) onto screen-printed electrodes, and following depositing glucose oxidase (GOx) by linking of glutaric dialdehyde. The interstitial fluid (ISF) extraction could be accelerated with assistance of reverse iontophoresis. The glucose in the ISF could react with GOx on the electrode to generate a current response of blood glucose concentration. The minimally measurement results for blood glucose showed an enhanced sensitivity of 0.934 μA/mM with a high correlation (R²&gt;0.99), low limit of detection (LOD ≈ 19.3 μM), broad linear detection range (LDR = 2–18 mM) that satisfices glucose detection application.</div></div>","PeriodicalId":279,"journal":{"name":"Colloids and Surfaces B: Biointerfaces","volume":"256 ","pages":"Article 115078"},"PeriodicalIF":5.6,"publicationDate":"2025-08-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144908033","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Advancing Shewanella-based whole-cell biosensors: A comprehensive review on heavy metal detection","authors":"Hui Xi ,&nbsp;Hui Wang ,&nbsp;Yu Li ,&nbsp;Xizi Long ,&nbsp;Xilin Li ,&nbsp;Yingying Sun ,&nbsp;Wendong Wang","doi":"10.1016/j.colsurfb.2025.115075","DOIUrl":"10.1016/j.colsurfb.2025.115075","url":null,"abstract":"<div><div>Whole-cell biosensors are widely used to detect the toxicity of heavy metals owing to their ease of culturing, low cost, and rapid response times. <em>Shewanella</em> species, electrically active microorganisms capable of extracellular electron transfer (EET), are often employed as sensitive bio-elements in biosensors. However, the EET efficiency and heavy metal tolerance of <em>Shewanella</em> species are influenced by various environmental factors, such as pH, carbon sources, and ion concentrations. These influences lead to challenges such as insufficient electron signal output and reduced tolerance or recovery after exposure to metal ions, compromising the stability and sensitivity of <em>Shewanella</em>-based biosensors. Notably, current <em>Shewanella</em>-based biosensors have demonstrated detection limits as low as 0.1 μM (Hg^2 +, Pb²⁺, Cd²⁺), with response times ranging from 5 min to 30 min depending on the sensor configuration. To address these issues, this review comprehensively examines the effects of key environmental factors and heavy metal exposure on the sensing performance of <em>Shewanella</em> spp. Furthermore, it highlights emerging strategies to enhance sensor performance, including the genetic engineering of <em>Shewanella</em> and material modifications to improve electron transport and overall sensor efficiency. This review provides novel insights into the principles underlying <em>Shewanella</em>-based toxicity biosensors through the lens of EET while also shedding light on recent advancements in the development of whole-cell biosensors utilizing <em>Shewanella</em> species.</div></div>","PeriodicalId":279,"journal":{"name":"Colloids and Surfaces B: Biointerfaces","volume":"256 ","pages":"Article 115075"},"PeriodicalIF":5.6,"publicationDate":"2025-08-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144907923","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Polycationic calixarene micellar aggregate as a novel iodophor platform for on-demand iodine release to bacteria","authors":"Loredana Ferreri ,&nbsp;Giuseppina D.G. Santonoceta ,&nbsp;Giovanna Ginestra ,&nbsp;Giuseppe Granata ,&nbsp;Nicola D'Antona ,&nbsp;Salvatore Petralia ,&nbsp;Antonia Nostro ,&nbsp;Carmelo Sgarlata ,&nbsp;Grazia M.L. Consoli","doi":"10.1016/j.colsurfb.2025.115071","DOIUrl":"10.1016/j.colsurfb.2025.115071","url":null,"abstract":"<div><div>Iodine is the most potent antiseptic agent used in clinical applications, but its volatility and toxicity are serious drawbacks that are stimulating the search of new strategies to achieve formulations stable at low iodine concentration. Here, we report that polycationic micellar aggregates, formed by the self-assembling of the amphiphilic choline-calix[4]arene derivative (CholCalix) in aqueous medium, are able to complex triiodide anions (I₃⁻) both in solution and in solid phase. This novel iodophor enhances the stability of 0.01 % iodine and 0.1 % povidone iodine (PVP-I) water solutions. The binding properties of the CholCalix aggregate towards iodine were investigated by UV–vis spectrophotometry, dynamic light scattering, and isothermal titration calorimetry. The bactericidal activity of the complexes was proved against a specimen of Gram-negative bacteria (<em>Escherichia coli</em> ATCC 10536) by time-killing assay. The molecular iodine release profile suggests that the calixarene-based micellar nanocarrier may succeed as a novel iodophor for on-demand I₂ release.</div></div>","PeriodicalId":279,"journal":{"name":"Colloids and Surfaces B: Biointerfaces","volume":"257 ","pages":"Article 115071"},"PeriodicalIF":5.6,"publicationDate":"2025-08-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144933608","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Long-term antifungal drug-delivery denture materials with plasma-initiated surface functionalization","authors":"Jianchuan Wen ,&nbsp;Parveez A. Abdul-Azees ,&nbsp;E. Matthew Lamb ,&nbsp;Tianhao Ming ,&nbsp;David D. Dean ,&nbsp;Xiao-Dong Chen ,&nbsp;Chih-Ko Yeh ,&nbsp;Yuyu Sun","doi":"10.1016/j.colsurfb.2025.115077","DOIUrl":"10.1016/j.colsurfb.2025.115077","url":null,"abstract":"<div><div>Conventional polymethylmethacrylate (PMMA)-based denture materials are susceptible to colonization and biofilm-formation by <em>Candida</em> species, leading to <em>Candida</em>-associated denture stomatitis (CADS), a recurring fungal infection that affects a large patient population. In this report, we developed a two-step, plasma-initiated surface functionalization approach to covalently bond poly (methacrylic acid) (PMAA) onto denture resins. Various reaction conditions were investigated and the effects of PMAA grafting yields on the physical properties of the resulting denture materials were determined. The presence of PMAA grafted onto the denture materials significantly increased their ability to bind miconazole, one of the most widely used drugs for managing CADS. Two <em>Candida</em> strains, SC5314 and UME6, were used as representative fungal species. Anticandidal drug released from the PMAA-grafted denture materials eradicated SC5314 and inhibited UME6 hyphae formation and growth. Colony forming unit and fluorescence microscopy confirmed that the anticandidal effects lasted 30 days, even after fungal re-inoculation. XTT assay demonstrated high biocompatibility of the modified materials. These findings suggest strong clinical potential of the new surface functionalized denture materials for preventing and managing CADS and other related fungal infections, offering a durable, biocompatible alternative for managing CADS and reducing fungal infections in denture wearers.</div></div>","PeriodicalId":279,"journal":{"name":"Colloids and Surfaces B: Biointerfaces","volume":"256 ","pages":"Article 115077"},"PeriodicalIF":5.6,"publicationDate":"2025-08-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144913897","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Stimuli-activable hollow Cu2O@C/N doped paste for the prevention of white spot lesions in orthodontic treatments","authors":"Kaixin Wang ,&nbsp;Yuanping Hao ,&nbsp;Mengyu Li ,&nbsp;Cunhui Fan ,&nbsp;Xiaojie Liu ,&nbsp;Xiangfu Meng ,&nbsp;Yang Liu","doi":"10.1016/j.colsurfb.2025.115073","DOIUrl":"10.1016/j.colsurfb.2025.115073","url":null,"abstract":"<div><div>White spot lesions (WSLs) are the most common complication of orthodontic treatment, compromising dental health and significantly affecting aesthetics. To address this clinical challenge, this study aims to develop a dual-functional therapeutic strategy that simultaneously promotes the remineralization of demineralized enamel and inhibits the activity of cariogenic bacteria, thereby achieving effective prevention and treatment of WSLs. A hollow double-shell structured Cu₂O@N/C nanozyme (H-Cu₂O@N/C) was synthesized using a one-step hydrothermal method. The thin nitrogen-doped carbon shell significantly enhances the material's stability in aqueous environments and reduces the systemic toxicity of cuprous oxide nanocrystals. By employing bioinspired encapsulation technology, H-Cu₂O@N/C was incorporated into a biodegradable adhesive matrix made of gelatin methacryloyl (GelMA) and sodium alginate, forming the Cu/GS composite ointment. The acidic plaque biofilm activates the peroxidase-like activity of H-Cu₂O@N/C, effectively catalyzing low-dose H₂O₂ into highly toxic hydroxyl radicals (·OH) for targeted antibacterial action. Crucially, Cu/GS acts as a nucleation template, crosslinking with abundant Ca^2 + to attract PO₄³⁻ from saliva, thereby promoting hydroxyapatite growth on the enamel surface and accelerating rapid remineralization. The Cu/GS ointment overcomes the limitations of existing single-function therapeutic materials through its synergistic “antibacterial-remineralization” dual-functional mechanism. In vitro biomimetic model experiments confirm its ability to simultaneously inhibit WSL progression and repair early-stage enamel defects, demonstrating significant potential for clinical intervention in orthodontic-related WSLs.</div></div>","PeriodicalId":279,"journal":{"name":"Colloids and Surfaces B: Biointerfaces","volume":"257 ","pages":"Article 115073"},"PeriodicalIF":5.6,"publicationDate":"2025-08-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144988722","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Bionanoformulated creams of silver (AgNPs), gold (AuNPs) and bimetallic silver-gold composites (Ag-AuNCPs) for tissue regeneration of MDR Pseudomonas aeruginosa-infected excision wounds in Wistar Rats","authors":"Richard Kolade Omole ,&nbsp;Nkem Torimiro ,&nbsp;Oluwole Isaac Adeyemi ,&nbsp;Muthupandian Saravanan ,&nbsp;Mohanraj Karthik Ganesh","doi":"10.1016/j.colsurfb.2025.115067","DOIUrl":"10.1016/j.colsurfb.2025.115067","url":null,"abstract":"<div><div>Multidrug-resistant (MDR) <em>Pseudomonas aeruginosa</em> (<em>P. aeruginosa</em>) is a Gram-negative pathogen with advanced antibiotic resistance and biofilm-forming machineries that cause delayed infections (especially in human chronic wounds). Novel therapeutics such as antibiofilm agents, antimicrobial peptides and nanoformulated creams are being developed to treat <em>P. aeruginosa</em>-infected wounds. Therefore, <em>Lysinibacillus fusiformis</em>-synthesized AgNPs, AuNPs and Ag-AuNCPs were incorporated into oil-in-water creams. These bionanoformulated creams were adopted for tissue regeneration of <em>P. aeruginosa</em>-infected excision wounds (250 mm² area) in Wistar rats (seven weeks old; 150–200 g). Histopathological examinations (haematoxylin and eosin staining) were performed on the regenerated skin tissues, and the biochemical mechanisms of action of the bionanoformulated creams were assessed. The AgNPs spectra peaked at 404 nm after 72 h, with a spherical shape of 18.42 nm in size, whereas the AuNPs spectra peaked at 546 nm after 48 h, with a spherical shape of 18.6 nm in size. The Ag-AuNCPs spectra recorded UV–vis λ<em>max</em> at 600.5 nm after 8 h with a spherically-clustered shape of 19.13 nm in size. The bionanoformulated creams of AgNPs, AuNPs, and Ag-AuNCPs regenerated the <em>P. aeruginosa</em>-infected tissues within 13 days, 15 days, and 11 days, respectively. Dermal collagen fibers, sebaceous glands, hair follicles, and epithelial glands were expressed in the regenerated tissues. These bionanoformulated creams reversed the oxidative stress induced by <em>P. aeruginosa</em> in the excised tissues, maintained the appropriate level of superoxide dismutase, and reduced lipid peroxidation in the tissues. This investigation makes a major contribution to public health research and global health advancement. These intriguing outcomes could be a blueprint for human clinical trials.</div></div>","PeriodicalId":279,"journal":{"name":"Colloids and Surfaces B: Biointerfaces","volume":"256 ","pages":"Article 115067"},"PeriodicalIF":5.6,"publicationDate":"2025-08-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144932267","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Mussel-Inspired Adhesive Macromolecule Modified Zinc Oxide Nanoparticles Incorporated into Polyethylene Glycol Hydrogels for Enhancing Infected Wound Healing","authors":"Xiangling Ye ,&nbsp;Zhilong Pi ,&nbsp;Meilian Wei ,&nbsp;Xu Changkui ,&nbsp;Zhichao Tan ,&nbsp;Yali Huan ,&nbsp;Chaolang Yan","doi":"10.1016/j.colsurfb.2025.115070","DOIUrl":"10.1016/j.colsurfb.2025.115070","url":null,"abstract":"<div><div>Infected wounds remain a major clinical challenge due to bacterial invasion, which disrupts the natural healing cascade through excessive reactive oxygen species (ROS) generation, severe vascular damage, and persistent inflammation. Inspired by the catechol-rich adhesive domains of mussel foot proteins, we developed an extracellular matrix (ECM)-mimetic polyethylene glycol (PEG) hydrogel incorporating polydopamine (PDA)-functionalized zinc oxide nanoparticles (ZnONPs) for infected wound therapy. The amino acid-functionalized PEG hydrogel reproduces ECM-like properties to facilitate cell migration and efficient exudate management; however, its lack of intrinsic antimicrobial activity limits therapeutic efficacy. To address this limitation, ZnONPs were coated with mussel-inspired PDA, replicating the adhesive and redox-active features of marine-derived macromolecules. PDA surface modification via amidation enhances hydrogel–tissue adhesion and enables sustained release of bioactive Zn²⁺ ions. <em>In vitro</em>, PEG/PDA@ZnONPs hydrogels achieved inhibition rates of 95.1 ± 1.00% against S. aureus and 95.30 ± 1.15% against E. coli, and scavenged ~79% of DPPH radicals, indicating potent antimicrobial and antioxidant properties. <em>In vivo</em>, the hydrogel modulated macrophage polarization by suppressing the pro-inflammatory M1 phenotype (CD163) and promoting the reparative M2 phenotype (INOS) and enhancing pro-healing signals, leading to 95.1% wound closure within 14 days compared to 64.8% in controls. Collectively, these findings highlight PDA-modified ZnONPs-loaded PEG hydrogels as a promising platform for advanced wound management.</div></div>","PeriodicalId":279,"journal":{"name":"Colloids and Surfaces B: Biointerfaces","volume":"257 ","pages":"Article 115070"},"PeriodicalIF":5.6,"publicationDate":"2025-08-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145004706","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}