Colloids and Surfaces B: Biointerfaces最新文献

{"title":"Combining lactate oxidase and metformin in cancer cell membrane-biomimetic liposomes for synergistic ferroptosis induction and hypoxia-alleviated cancer therapy","authors":"Cheng-Lei Li ,&nbsp;Feng-Ming Li ,&nbsp;Ke-Ke Feng ,&nbsp;Yong-Shan Hu ,&nbsp;Yi-Fan Tu ,&nbsp;Shi-Cheng Tian ,&nbsp;Zhen-Hua Liu ,&nbsp;Jing-Wei Shao","doi":"10.1016/j.colsurfb.2026.115420","DOIUrl":"10.1016/j.colsurfb.2026.115420","url":null,"abstract":"<div><div>Ferroptosis, an iron-dependent form of regulated cell death driven by lipid peroxide accumulation, offers a promising strategy to circumvent tumor drug resistance. However, solid tumors exhibit intrinsic resistance to ferroptosis due to elevated antioxidant systems and hypoxic microenvironments, limiting H₂O₂-mediated lipid peroxidation. Herein, we developed a tumor-homing liposomal platform (ML-MLip) for co-delivery of lactate oxidase (LOX) and metformin (MET) to leverage the tumor microenvironment for enhanced ferroptosis induction. LOX catalyzes intratumoral abundant lactate (due to the Warburg effect) to generate H₂O₂, while MET alleviates tumor hypoxia by inhibiting mitochondrial complex I, ensuring sufficient oxygen for LOX activity. Concurrently, MET suppresses SLC7A11-mediated GSH synthesis and disabling GPX4-mediated lipid peroxide detoxification. Soybean phosphatidylcholine (SPC) within ML-MLip provides polyunsaturated lipids as substrates for peroxidation, overcoming endogenous lipid shortages. <em>In vitro</em> and <em>in vivo</em> studies demonstrated that ML-MLip preferentially accumulated at tumor sites, triggering iron-dependent lipid peroxidation (LPO) and ferroptosis. Additionally, lactate depletion and ferroptosis reversed the tumor immunosuppressive microenvironment, promoting the release of tumor antigens and damage-associated molecular patterns (DAMPs) to augment systemic immune responses. This strategy converts tumor-specific metabolites (lactate) and ions (iron) into therapeutic effectors, offering a microenvironment-responsive approach for synergistic ferroptosis-immunotherapy against malignant tumors.</div></div>","PeriodicalId":279,"journal":{"name":"Colloids and Surfaces B: Biointerfaces","volume":"261 ","pages":"Article 115420"},"PeriodicalIF":5.6,"publicationDate":"2026-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145975459","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Luteolin-loaded nanocomposites target microglia for sepsis-associated encephalopathy therapy via intranasal delivery","authors":"Weihong Guo ,&nbsp;Tao Liu ,&nbsp;Xiaojia Peng ,&nbsp;Jiafa Zhang ,&nbsp;Zhefeng Chen ,&nbsp;Chongshuang Xia ,&nbsp;Canhong Yang ,&nbsp;Shuting Xie ,&nbsp;Tianming Lü","doi":"10.1016/j.colsurfb.2026.115433","DOIUrl":"10.1016/j.colsurfb.2026.115433","url":null,"abstract":"<div><div>Sepsis-associated encephalopathy (SAE) is a neurological dysfunction resulting from sepsis, with neuroinflammation identified as a key pathogenic mechanism. Luteolin (LUT) is a dietary polyphenol that possesses considerable therapeutic potential. However, its application is hindered by poor aqueous solubility, low absorption, and rapid metabolism. To address these challenges, we developed luteolin-loaded lactoferrin-chondroitin sulfate nanoparticles (LUT-LF-CS NPs) to enhance the bioavailability of LUT and evaluate its efficacy in alleviating neuroinflammation in SAE. The optimized LUT-LF-CS NPs exhibited a uniform particle size of 45.9 nm and an encapsulation efficiency of 71.73 %. These nanocomposites demonstrated excellent cytocompatibility and enhanced the intrinsic antioxidant and anti-inflammatory activities of LUT. Furthermore, they facilitated cellular uptake in microglia through CD44 receptor-mediated endocytosis, enabling effective drug delivery to the cerebral cortex after intranasal administration. Mechanistically, LUT-LF-CS NPs promoted the polarization of microglia from the pro-inflammatory M1 phenotype to the anti-inflammatory M2 phenotype while inhibiting the TLR4/MyD88/NF-κB signaling pathway. In conclusion, LUT-LF-CS NPs significantly enhance the neuroprotective effects of LUT, positioning them as a promising therapeutic strategy for SAE.</div></div>","PeriodicalId":279,"journal":{"name":"Colloids and Surfaces B: Biointerfaces","volume":"261 ","pages":"Article 115433"},"PeriodicalIF":5.6,"publicationDate":"2026-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146028004","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Osteoinductive sandwich-structured HA/PEEK implant for rapid critical-size skull repair","authors":"Mei-li Qi ,&nbsp;Kunshan Yuan ,&nbsp;Enhui Song ,&nbsp;Xiangyi Feng ,&nbsp;Tianheng Lu ,&nbsp;Lin Zhao ,&nbsp;Haihong Guo ,&nbsp;Haijun Zhang","doi":"10.1016/j.colsurfb.2026.115428","DOIUrl":"10.1016/j.colsurfb.2026.115428","url":null,"abstract":"<div><div>Critical-size bone defects afflict millions annually, driving a cascade of inflammation and incomplete tissue regeneration. Polyetheretherketone (PEEK) remains the material of choice for load-bearing implants because its modulus mirrors that of cortical bone; yet its notorious bioinertness curtails both anti-inflammatory signaling and osseointegration, stalling the healing process. To break this stalemate, we designed a sandwich-structured, osteoinductive hydroxyapatite (HA)/PEEK implant tailored for skull repair. A porous PEEK core was engineered via the salting out technique and its surface was homogeneously functionalized with HA through simple physical blending, preserving the implant’s open porosity while imparting potent osteoinductive activity. In a rabbit critical calvarial defect (10 mm diameter), new bone advanced from the periphery to the center, achieving full osseous continuity within only 4 months and markedly surpassing unmodified PEEK. The robust bone-implant integration demonstrated by this sandwich HA/PEEK construct not only converts a bioinert polymer into an osteoinductive implant but also may expand treatment options for growing children, offering a clinically translatable solution for skull repair after trauma, hemorrhage, tumor resection, or congenital dysplasia.</div></div>","PeriodicalId":279,"journal":{"name":"Colloids and Surfaces B: Biointerfaces","volume":"261 ","pages":"Article 115428"},"PeriodicalIF":5.6,"publicationDate":"2026-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145915403","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Gallic- and ω-3-linolenic-acids-mediated MgO nanoplates enable band-edge tuning and ROS-driven anticancer activity","authors":"Ankita Thakur ,&nbsp;Ahmed Ahmed Ibrahim ,&nbsp;Khalid Mujasam Batoo ,&nbsp;Jyoti Gaur ,&nbsp;Kasim Sakran Abass ,&nbsp;Sanjeev Kumar","doi":"10.1016/j.colsurfb.2026.115455","DOIUrl":"10.1016/j.colsurfb.2026.115455","url":null,"abstract":"<div><div>Green nanotechnology seeks earth-abundant, low-toxicity oxides that can deliver biomedical benefits with minimal environmental impact. We report the first magnesium oxide (MgO) nanoparticles (NPs) synthesised exclusively from Pinus patula needle waste via a one-pot, aqueous route that requires no organic solvents or post-calcination. Targeted GC–MS profiling pinpoints gallic acid, ω-3 linolenic-acid bis-TMS ether and three mono-linoleoylglycerol derivatives as the dominant reductive/capping agents (Σ &gt; 35 % TIC), while FT-IR confirms they are in-situ chelation to Mg²⁺. The renewable extract simultaneously reduces Mg²⁺, caps nascent nuclei and templates mesoporosity, affording a 78 % yield of well-crystalized platelets (XRD domain 55 ± 3 nm; BET surface area 54 m² g⁻¹; pore diameter 3.8 nm). Oxygen-vacancy enrichment contracts the bulk 7.8 eV gap to an indirect 3.24 eV; the resulting band edges (–0.29 V and +1.81 V vs NHE, pH 7) favour superoxide but suppress hydroxyl-radical formation, enabling stimulus-dependent redox switching. In vitro, the particles trigger intrinsic apoptosis in MCF-7 cells with an IC₅₀ of 71 ng mL⁻¹ and a 32-fold Bax/Bcl-2 shift, yet exhibit negligible haemolysis toward erythrocytes. Antioxidant assays reveal a DPPH•-scavenging IC₅₀ of 25 µg mL⁻¹ lower than any green-synthesised MgO reported to date. Comprehensive GC-MS, FT-IR and time-resolved XRD tracking link the vacancy stabilization and ripening inhibition to the coordinated gallic-acid/flavolipid corona.</div></div>","PeriodicalId":279,"journal":{"name":"Colloids and Surfaces B: Biointerfaces","volume":"261 ","pages":"Article 115455"},"PeriodicalIF":5.6,"publicationDate":"2026-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145996861","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Conformal polypyrrole biointerfaces on porous PHA monoliths via oxidative chemical vapor deposition","authors":"Adriana Kovalcik ,&nbsp;Nicole Cernekova ,&nbsp;Fika Fauzi ,&nbsp;Ranjita K. Bose ,&nbsp;Zdenko Spitalsky ,&nbsp;Zuzana Kadlecova ,&nbsp;Lucy Vojtova ,&nbsp;Zdenka Víchová ,&nbsp;Petr Humpolíček ,&nbsp;Patrycja Bober","doi":"10.1016/j.colsurfb.2026.115417","DOIUrl":"10.1016/j.colsurfb.2026.115417","url":null,"abstract":"<div><div>Chronic wounds require dressings that manage exudate, conform to soft tissue, provide mechanical support, and deliver intrinsic bioactivity. Here, we report conductive, hydrogel-like porous polyhydroxyalkanoate (PHA) monoliths dressings coated with polypyrrole (PPy) using oxidative chemical vapor deposition (oCVD). Porous PHA substrates were prepared from polyhydroxybutyrate (PHB) and a P4HB-containing copolymer by thermally induced phase separation and were uniformly functionalized throughout their three-dimensional architecture by this solvent-free process. The resulting PHA/PPy porous monoliths combine high water uptake with electrical conductivity and biological activity. They exhibit a swelling ratio of ∼250 %, maintaining a moist environment while preserving viscoelastic integrity. Sheet resistance ranges from 26 to 86 kΩ/sq, enabling platforms for electrical sensing in tissue repair. The composites do not induce cytotoxicity and exhibit intrinsic radical-scavenging capacity and antibacterial activity against both <em>Gram-positive</em> and <em>Gram-negative</em> bacteria. These properties are achieved without chemical derivatization of the PHA matrix. The hydrophobic PHA core provides mechanical robustness, while the conformal PPy layer imparts conductivity and bioactivity. Overall, this oCVD route provides a scalable, solvent-free strategy to engineer multifunctional, hydrogel-like porous monolith dressings that integrate moisture management, mechanical resilience, electrical conduction, and inherent antioxidant and antimicrobial activity. These features position the developed materials as promising bioactive and bioelectronic wound dressings and soft tissue interfaces.</div></div>","PeriodicalId":279,"journal":{"name":"Colloids and Surfaces B: Biointerfaces","volume":"261 ","pages":"Article 115417"},"PeriodicalIF":5.6,"publicationDate":"2026-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145964868","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Characterizations and controlled drug release behavior of acyclovir-loaded starch-based microneedles patches for transdermal herpes simplex virus therapy","authors":"Kyeongjung Kim ,&nbsp;Se-woon Choe ,&nbsp;Jae-Young Je ,&nbsp;Min-Jin Hwang ,&nbsp;Yoonhang Lee ,&nbsp;Do-Hyung Kim ,&nbsp;Kwangcheol Casey Jeong ,&nbsp;Soon-Do Yoon","doi":"10.1016/j.colsurfb.2026.115430","DOIUrl":"10.1016/j.colsurfb.2026.115430","url":null,"abstract":"<div><div>Transdermal drug delivery systems (TDDS) using microneedles (MNs) patches have shown promise for improved therapeutic outcomes. In this study, acyclovir (ACV)-loaded MNs patches for herpes simplex virus (HSV) therapy were prepared using mungbean starch (MBS), polyvinyl alcohol (PVA), and plasticizers (arginine and mannitol), and their physicochemical properties, ACV release behavior, antimicrobial activity, and biodegradation, cell viability, and antiviral efficacy were investigated. The MNs exhibited compression forces of 1.30 – 4.80 N/needle and a pyramidal square shape with a length of 620–640 μm, ensuring efficient skin penetration. The ACV release (%) from the ACV-loaded MNs patches during an artificial skin test was found to be 2.50 – 4.32 times higher than that from ACV-loaded biomaterial as the film-type formulation. Additionally, over 98.0 % of ACV was released from the prepared MNs patches within 80 min. The ACV release mechanism was analyzed using zero-order, first-order, Higuchi, Fickian diffusion, and Korsmeyer-Peppas models, which revealed a Fickian diffusion mechanism. Visualization of intradermal drug release were conducted using ACV- and riboflavin-loaded MNs patches on agar blocks and pig ears/agar block models. Biodegradability, cell viability, and antiviral studies further demonstrated the potential of MNs patches as a TDDS. These results suggest that the prepared MNs patches are promising candidates for transdermal HSV therapy.</div></div>","PeriodicalId":279,"journal":{"name":"Colloids and Surfaces B: Biointerfaces","volume":"261 ","pages":"Article 115430"},"PeriodicalIF":5.6,"publicationDate":"2026-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145964875","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Recent advances in metal-organic framework-based nanozymes for cancer theranostics driven by synthetic innovation and machine learning design","authors":"Sangeeta Yadav ,&nbsp;Aditi Sarkar ,&nbsp;Saurabh Shivalkar ,&nbsp;Fiza Fatima ,&nbsp;Siddharth Kumar Thakur ,&nbsp;Ankita Chaudhary ,&nbsp;Sintu Kumar Samanta ,&nbsp;Amaresh Kumar Sahoo","doi":"10.1016/j.colsurfb.2026.115463","DOIUrl":"10.1016/j.colsurfb.2026.115463","url":null,"abstract":"<div><div>Nanozymes are engineered nanomaterials designed at the atomic scale to fine-tune their structure, composition, and electronic properties, thereby creating active sites that mimic those of natural enzymes. Among these materials, Metal-organic frameworks (MOFs) are notable for their well-defined, porous frameworks, which are created by connecting metal ions or clusters with organic linkers. Their large surface area, adjustable porosity, and superior biocompatibility enable excellent catalytic activity. By containing specific catalytic functionalities, MOF-based nanozymes can mimic peroxidase, oxidase, catalase, and superoxide dismutase activities. These properties make them genuinely promising for biomedical applications, particularly in cancer diagnosis and therapy. Recent progress in synthetic design, post-synthetic modification, and machine learning–assisted optimization has enhanced their structural precision and catalytic efficiency. Furthermore, MOFs serve as multifunctional therapeutic platforms capable of supporting combined treatment strategies and producing synergistic therapeutic effects, thereby establishing their potential as next-generation systems for targeted cancer treatment and diagnostic integration.</div></div>","PeriodicalId":279,"journal":{"name":"Colloids and Surfaces B: Biointerfaces","volume":"261 ","pages":"Article 115463"},"PeriodicalIF":5.6,"publicationDate":"2026-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146074301","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Corrigendum to “Biomimetic oral drug delivery: Translating nature’s design into therapeutic innovation” [Colloids Surf. B: Biointerfaces 259 (2026) 115348]","authors":"Muzaffaruddin Ahmed Madny ,&nbsp;Khushwant S. Yadav","doi":"10.1016/j.colsurfb.2026.115502","DOIUrl":"10.1016/j.colsurfb.2026.115502","url":null,"abstract":"","PeriodicalId":279,"journal":{"name":"Colloids and Surfaces B: Biointerfaces","volume":"261 ","pages":"Article 115502"},"PeriodicalIF":5.6,"publicationDate":"2026-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146111801","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Naringenin nanosuspensions embedded glycyrrhizin-based hydrogel ameliorates cholestatic liver injury in mice by inhibiting oxidative stress and HMGB1-mediated inflammation","authors":"Qiqi Li ,&nbsp;Yaochen Deng ,&nbsp;Xiaolu Han ,&nbsp;Yuhan Dong ,&nbsp;Xianggen Wu ,&nbsp;Wei Zhu ,&nbsp;Meixing Yan","doi":"10.1016/j.colsurfb.2026.115466","DOIUrl":"10.1016/j.colsurfb.2026.115466","url":null,"abstract":"<div><div>Naringenin (NAR) possesses remarkable hepatoprotective potential. However, its extremely low aqueous solubility and oral bioavailability greatly constrain its therapeutic efficacy. To overcome these limitations, we developed a novel oral nanodelivery system, NanoNAR@Glycygel, by embedding NAR nanosuspensions (NanoNAR) into a self-assembled glycyrrhizin-based hydrogel (Glycygel). The design of this delivery system improves solubility, enhances absorption, and provides synergistic hepatoprotective effects. NanoNAR, when stabilized by the natural biosurfactant glycyrrhizin, exhibited a uniform particle size of approximately 230 nm and showed markedly improved solubility in physiologically relevant media. The hydrogel network formed by Glycygel effectively encapsulated NanoNAR, further enhancing its solubility and controlled release behavior. Pharmacokinetic analyses revealed that NanoNAR@Glycygel significantly enhanced the oral bioavailability of NAR and increased its hepatic accumulation, demonstrating how the synergistic interplay between nanonization and the glycyrrhizin hydrogel matrix facilitates rapid absorption and sustained release. In a cholestatic liver injury mouse model, NanoNAR@Glycygel treatment markedly alleviated cholestasis and hepatic histopathological damage, restoring liver morphology and serum biochemical parameters to near-normal levels. Mechanistic investigations revealed for the first time that HMGB1 signaling is involved in this cholestatic liver injury, and NanoNAR@Glycygel exerted its potent therapeutic effect by inhibiting this signaling. The NanoNAR@Glycygel cloud also reduced malondialdehyde (MDA) levels and enhanced superoxide (SOD) activity, thereby mitigating oxidative injury. Collectively, these findings demonstrate that NanoNAR@Glycygel is a safe, simple, and highly effective oral delivery platform that not only unleashes the therapeutic potential of NAR but also highlights the distinctive advantages of glycyrrhizin-based matrices for the targeted oral delivery of hydrophobic natural bioactives.</div></div>","PeriodicalId":279,"journal":{"name":"Colloids and Surfaces B: Biointerfaces","volume":"261 ","pages":"Article 115466"},"PeriodicalIF":5.6,"publicationDate":"2026-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146035620","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Membrane-mimicking surfaces modulate the heme pocket structure and oxygen affinity in myoglobin: A surface-enhanced resonance Raman study","authors":"Ulises A. Zitare ,&nbsp;Andresa Messias ,&nbsp;Santiago Di Lella ,&nbsp;Magalí F. Scocozza ,&nbsp;Darío A. Estrin ,&nbsp;Luciana Capece ,&nbsp;Daniel H. Murgida","doi":"10.1016/j.colsurfb.2026.115457","DOIUrl":"10.1016/j.colsurfb.2026.115457","url":null,"abstract":"<div><div>In this study, we investigated how membrane-mimicking surfaces modulate the structure and oxygen affinity of myoglobin (Mb) using surface-enhanced resonance Raman spectroscopy under electrochemical control. Nanostructured silver electrodes functionalized with self-assembled monolayers of varying charge and composition were employed to model basic features of the outer mitochondrial membrane in a controlled fashion. Molecular dynamics simulations show that the interactions of Mb with these surfaces are mediated by lysine-rich domains in helix F and the CD region. These interactions induce subtle structural changes in Mb’s heme pocket, of both the ferric and ferrous protein, that imply the loss of the proximal ligand, water and molecular oxygen, respectively. The fraction of ferrous OxyMb that retains the oxygen ligand in the biomimetic complexes exhibits spectral features consistent with a weakened Fe–O₂ bond and decreased O₂ affinity. Overall, our findings show that Mb’s transient interaction with a mitochondrial membrane model facilitates O₂ release through fine structural modulation, providing a possible molecular basis for how such interactions might influence oxygen delivery in cells.</div></div>","PeriodicalId":279,"journal":{"name":"Colloids and Surfaces B: Biointerfaces","volume":"261 ","pages":"Article 115457"},"PeriodicalIF":5.6,"publicationDate":"2026-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146016837","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}