Colloids and Surfaces B: Biointerfaces最新文献

{"title":"Precision targeting of ALK-positive lung cancer: Engineering HFN@MS4078 nanocages for optimized PROTAC delivery","authors":"Yuan Fan ,&nbsp;Yuxiang Liu ,&nbsp;Liujiao Wang ,&nbsp;Huashan Xu ,&nbsp;Lele Yu ,&nbsp;Yue Lang ,&nbsp;Huafei Li ,&nbsp;Fuxue Chen ,&nbsp;Shini Feng","doi":"10.1016/j.colsurfb.2025.114974","DOIUrl":"10.1016/j.colsurfb.2025.114974","url":null,"abstract":"<div><div>Protein hydrolysis-targeted chimeras (PROTACs) are heterobifunctional molecules designed for the selective degradation of target proteins. MS4078, a novel PROTAC, shows promise for treating ALK-positive non-small cell lung cancer (NSCLC), yet its clinical efficacy is hindered by suboptimal tumor targeting and off-target toxicities. To enhance its therapeutic profile, we developed human heavy chain ferritin (HFN) as a targeted delivery system, leading to the creation of HFN@MS4078 nanocages. Prepared via passive loading, HFN@MS4078 achieved a drug loading capacity of 178 molecules per nanocage with a HFN recovery rate of 70.1 %. The nanocages exhibited a rapid release profile under lysosomal conditions (pH 5.0), with approximately 80 % drug release after 60 h, while maintaining over 80 % stability at physiological pH 7.4. In vitro, HFN@MS4078 was actively internalized into lysosomes via TfR1 receptor binding, resulting in a 2.7–2.8 times reduction in IC₅₀ values compared to free MS4078. Furthermore, HFN@MS4078 significantly decreased the expression levels of ALK, p-ALK, p-AKT, and p-ERK in NCI-H2228 and NCI-H3122 cell lines. In vivo, HFN@MS4078 substantially reduced tumor volume and prolonged survival while exhibiting negligible systemic toxicity. These results suggest that HFN@MS4078 represents a promising strategy for targeted therapy in ALK-positive NSCLC, enhancing therapeutic efficacy while minimizing systemic toxicity.</div></div>","PeriodicalId":279,"journal":{"name":"Colloids and Surfaces B: Biointerfaces","volume":"255 ","pages":"Article 114974"},"PeriodicalIF":5.4,"publicationDate":"2025-07-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144695289","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Development of a cationic photodynamic antibacterial agent based on gallic acid and ethylene glycol diglycidyl ether and enriched with hydroxyl groups for effective antimicrobial therapy","authors":"Hao Zhou ,&nbsp;Zhiwen Qi ,&nbsp;Hongxia Chen ,&nbsp;Xingying Xue ,&nbsp;Lingyan Liu ,&nbsp;Chengzhang Wang","doi":"10.1016/j.colsurfb.2025.114973","DOIUrl":"10.1016/j.colsurfb.2025.114973","url":null,"abstract":"<div><div>In recent years, various antibiotic-resistant bacterial species have emerged, thereby complicating bacterial infection treatment. Hence, the development of nontraditional, multifunctional, cationic photodynamic antibacterial agents is imperative and valuable to inhibit normal and multidrug-resistant bacterial strains. Here, by performing ring-opening reactions, we successfully synthesized an antibacterial polycation (EY-QEGDM-MG) based on gallic acid, ethylene glycol diglycidyl ether, and eosin Y and enriched with various functional components, namely a photosensitizer, quaternary ammonium (QA), and hydroxyl species. Under light irradiation, the polymer inhibited the growth of both <em>Escherichia coli</em> and methicillin-resistant <em>Staphylococcus aureus</em> because of the combined antibacterial activities of the photosensitizer and the QA group. Evaluation of the mechanism of the antibacterial effect revealed that the polymer binds to the bacterial cell surface and irreversibly damages the cell wall and membrane structure. Furthermore, the polymer also exhibited low cytotoxicity and good hemocompatibility. The antibacterial efficacy of the polymer was assessed <em>in vivo</em> on a rat wound infection model; the polymer showed sustainably enhanced therapeutic effects. Alterations in the levels of CD31-expressing cells and CD68⁺ macrophages and results of histological analyses validated that the polymer could inhibit bacterial infection in the mice model. The present study provides an efficient approach to develop an effective antimicrobial agent to reduce hospital-acquired bacterial infections.</div></div>","PeriodicalId":279,"journal":{"name":"Colloids and Surfaces B: Biointerfaces","volume":"256 ","pages":"Article 114973"},"PeriodicalIF":5.4,"publicationDate":"2025-07-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144711052","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Robotic manipulations of single cells using a large-volume piezoelectric micropipette with nanoliter precision","authors":"Boglarka Kovacs ,&nbsp;Szabolcs Novak ,&nbsp;Igor Sallai ,&nbsp;Beatrix Magyarodi ,&nbsp;Inna Szekacs ,&nbsp;David Selmeczi ,&nbsp;Balint Szabo ,&nbsp;Robert Horvath","doi":"10.1016/j.colsurfb.2025.114972","DOIUrl":"10.1016/j.colsurfb.2025.114972","url":null,"abstract":"<div><div>Single-cell manipulations are a limiting factor in single-cell omics (genomics, transcriptomics, proteomics), in vitro fertilization, and cloning. Cellular adhesion force often plays a pivotal role in various biological contexts, spanning from lower organisms to the human body. Investigating the mechanism of adhesive interactions at the individual cell level holds significant importance. We used a computer-controlled piezoelectric micropipette (NanoPick) built onto an inverted microscope, offering subnanoliter precision liquid handling in the range of 0.1–600 nanoliters with a temporal resolution of 1 millisecond. In contrast to previous pipette-based cell manipulations, in our device, phase contrast and fluorescent imaging of the microscope was not limited by the micropipette. Moreover, this compact setup efficiently enabled single-cell detection, targeting, picking, and isolation without fluidic tubes and syringes. We investigated the integrin-mediated adhesion between an RGD (Arg-Gly-Asp) motif displaying surface and the HeLa Fucci tumor cell line. Using a 70 µm inner diameter micropipette, we found that increasing the pipetting speed (voltage ramp rate applied on the piezoelectric head) improved the cell picking success rate to almost 100 %. Although the more strongly attached unmodified HeLa cells could not be picked up even at the highest flow rates. However, vibrating the fluid in the micropipette successfully detached fully flattened cells without any biochemical treatment. This vibration micropipetting method enabled the detachment of 79.9 % of the strongly adherent HeLa cells, preserving mechanical integrity for downstream omics analyses despite a loss in viability. Compared to valve-controlled systems, NanoPick demonstrated higher efficiency and precision, particularly in handling THP-1 cells. Its rigid design minimized transient delays, allowing time-dependent flow profiles and enhanced detachment at lower flow rates. Our method allows adhesion measurements on hundreds of cells and offers precise control over fluid volume and timing, suitable for manipulating adherent cells or larger objects such as organoids, spheroids, oocytes, or larvae. The introduced vibration micropipetting method could be employed for the mechanical stimulation of single cells.</div></div>","PeriodicalId":279,"journal":{"name":"Colloids and Surfaces B: Biointerfaces","volume":"256 ","pages":"Article 114972"},"PeriodicalIF":5.4,"publicationDate":"2025-07-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144711150","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Polydopamine immobilized icariin on 3D printed porous polylact-glycolic acid/calcium sulfate scaffold to improve osteogenesis","authors":"Kunpeng Ma ,&nbsp;Rongfeng Zhuo ,&nbsp;Zhilong Pi ,&nbsp;Jiaqi Zhang ,&nbsp;Xin Peng ,&nbsp;Xiangling Ye ,&nbsp;Xiangyang Ma ,&nbsp;Ying Zhang","doi":"10.1016/j.colsurfb.2025.114976","DOIUrl":"10.1016/j.colsurfb.2025.114976","url":null,"abstract":"<div><div>Bone defects resulting from fractures, bone tumor resections, and non-unions are common clinical challenges. 3D-printed bone scaffolds offer enhanced customization and adaptability. however, their existing limitations in biological activity need to be addressed. In this study, we developed a novel composite scaffold (PLGA/CaSO4/PDA/ICA) for bone defect repair by incorporating polydopamine (PDA) and icariin (ICA) into a PLGA/CaSO4 matrix. We utilized Fused Deposition Modeling (FDM) technology to fabricate PLGA/CaSO₄ scaffolds and employed polydopamine (PDA) for the effective loading of ICA. Characterization tests revealed that the PLGA/CaSO₄/PDA/ICA scaffolds exhibited excellent hydrophilicity and mechanical properties. In vitro experiments showed that the PLGA/CaSO₄/PDA/ICA scaffolds significantly promoted cell proliferation, migration, and osteogenic differentiation while enhancing the expression of osteogenesis-related genes. Additionally, in vivo experiments revealed accelerated bone regeneration in the PLGA/CaSO4/PDA/ICA group, displaying higher bone volume fraction (BV/TV), denser trabeculae, and deeper scaffold integration at 8 weeks. Overall, These findings highlight the synergistic role of PDA-mediated surface modification and ICA-driven osteoinduction, positioning the PLGA/CaSO4/PDA/ICA scaffold as a promising candidate for bone tissue engineering applications.</div></div>","PeriodicalId":279,"journal":{"name":"Colloids and Surfaces B: Biointerfaces","volume":"256 ","pages":"Article 114976"},"PeriodicalIF":5.6,"publicationDate":"2025-07-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144738416","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Nanofibers encapsulating drug-loaded hyper-crosslinked hollow porous polymeric nanospheres: A multifunctional membrane for wound healing","authors":"Qian Liao ,&nbsp;Qianqing Fan ,&nbsp;Yuheng Wen ,&nbsp;Chengyao Xue ,&nbsp;Yang Lu ,&nbsp;Qing Hou ,&nbsp;Deng-Guang Yu ,&nbsp;Wenliang Song ,&nbsp;Jiaxiang Ye","doi":"10.1016/j.colsurfb.2025.114967","DOIUrl":"10.1016/j.colsurfb.2025.114967","url":null,"abstract":"<div><div>Developing stable, effective, and processable bactericidal materials is an important challenge in combating bacterial infections in various biomedical and environmental fields. Hyper-crosslinked hollow porous nanospheres (HHPNs) have attracted increasing attention as control and release medium for insoluble and unstable drugs due to transmit properties original from their adjustable pore structure and surface functionality. However, to assemble hollow porous spheres is complicated while difficult to use in applications due to their powdery nature. To broaden the usability of powdered polymers, an approach that integrates a bottom-up self-assembly strategy with top-down electrospinning was introduced, allowing for the efficient fabrication of nanofibrous membranes from self-assembled HHPNs. The HHPNs were synthesized via an one-step Friedel−Crafts reaction without templating methods, yielding customizable pore structures with high antibacterial agent loading capacities. Resveratrol (Res) loaded HHPNs were further processed into polycaprolactone nanofibrous membranes (Res@HHPNs/PCL NFMs) by electrospinning, which exhibited sustainable antimicrobial effects against Staphylococcus aureus (<em>S. aureus</em>) and Escherichia coli (<em>E. coli</em>). <em>In vivo</em> experiments indicated that these antimicrobial and antioxidant NFMs promoted wound healing by reducing the expression of inflammatory factors (including IL-1β and TNF-α) and enhancing angiogenesis at the wound site. In conclusion, the Res@HHPNs/PCL NFMs are user-friendly, dual functionality, and their recycling process effectively prevents secondary environmental pollution. This study highlights the utility of HHPNs for preparing NFMs and provides a new avenue for the potential development of antimicrobial membranes in biomedical and environmental fields.</div></div>","PeriodicalId":279,"journal":{"name":"Colloids and Surfaces B: Biointerfaces","volume":"255 ","pages":"Article 114967"},"PeriodicalIF":5.4,"publicationDate":"2025-07-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144695175","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Extracellular vesicle encapsulated mesoporous molecularly imprinted silica for peptide drug delivery","authors":"Meixin Wang ,&nbsp;Xingguo Liu ,&nbsp;Jingyuan Song ,&nbsp;Liping Wang ,&nbsp;Lianghai Hu","doi":"10.1016/j.colsurfb.2025.114971","DOIUrl":"10.1016/j.colsurfb.2025.114971","url":null,"abstract":"<div><div>Peptide therapeutics have demonstrated remarkable efficacy in the treatment of metabolic disorders (GLP-1 analogs), cancers (targeted tumor receptor inhibition), and infectious diseases (antimicrobial/viral entry blockade mechanisms), yet their clinical application remains constrained by rapid in vivo degradation and limited transmembrane permeability due to macromolecular polarity. To address these challenges, we developed a biomimetic nano-delivery system (EV@DhHP-6-MIP) synergizing milk-derived extracellular vesicles (EVs) with molecularly imprinted mesoporous silica through ultrasound-assisted membrane fusion technology. The molecularly imprinted mesoporous silica, featuring surface-imprinted binding sites on its ordered 3.1 ± 0.3 nm channels, achieved high-efficiency loading of the antioxidative peptide DhHP-6. The EVs enhanced targeted delivery via CD63/TSG101 transmembrane protein-mediated receptor-dependent endocytosis, while their natural phospholipid bilayer structure minimized immunogenicity compared to synthetic carriers. Leveraging milk as a renewable resource, this system ensured scalable production feasibility. In vitro evaluation using human embryonic kidney 293 T cells—selected for their well-characterized endocytic mechanisms and stable oxidative stress response—demonstrated that EV@DhHP-6-MIP reduced intracellular ROS levels by 38.5 %. For in vivo validation, <em>Caenorhabditis elegans</em>, which shares evolutionarily conserved oxidative stress pathways with humans, was employed. EV@DhHP-6-MIP treatment extended nematode survival time by 31.2 % under paraquat-induced oxidative stress and reduced intestinal lipofuscin accumulation by 40.47 %, without observable reproductive toxicity. This system resolves the longstanding conflict between protective encapsulation and bioavailability enhancement in peptide drug delivery through a tripartite synergistic mechanism: mesoporous silica-enabled drug stabilization, molecular imprinting-driven controlled release, and EV-mediated efficient delivery. Our work establishes a theoretical paradigm for developing intelligent nano-drug delivery systems with high efficacy and low toxicity, thereby holding significant translational value.</div></div>","PeriodicalId":279,"journal":{"name":"Colloids and Surfaces B: Biointerfaces","volume":"255 ","pages":"Article 114971"},"PeriodicalIF":5.4,"publicationDate":"2025-07-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144687087","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Artificial intelligence-assisted next-generation biomaterials: From design and preparation to medical applications","authors":"Bixia Zhou ,&nbsp;Xin Li ,&nbsp;Yuchen Pan ,&nbsp;Bingfang He ,&nbsp;Bingbing Gao","doi":"10.1016/j.colsurfb.2025.114970","DOIUrl":"10.1016/j.colsurfb.2025.114970","url":null,"abstract":"<div><div>The Fourth Industrial Revolution (Industry 4.0) has marked a shift from traditional materials to the era of smart materials. The integration of artificial intelligence (AI) with biomaterials is transforming the biosensing and biomedical fields. Although AI-assisted biomaterial manufacturing holds significant promise, the design and synthesis of smart materials remain in the early stages. To accelerate the implementation of AI-assisted biomaterials in fields such as biomedicine and biological intelligent systems, various algorithms have been developed to predict material properties, enable material de novo design, and establish a foundation for the development of next-generation multifunctional biomaterials. This review presents a comprehensive overview of AI-assisted biomaterial design, property prediction, fabrication, and potential biomedical applications. Recent advances in AI-driven protein engineering relevant to materials science are summarized, followed by an analysis of AI's role in designing, predicting, and optimizing next-generation biomaterials. The influence of AI-assisted systems on the structural and functional properties of biosmart materials is also explored. Applications such as therapeutic diagnostics, electronic skin (e-skin), biosensing, and other biomedical technologies are highlighted. Finally, current challenges and future perspectives are discussed, with emphasis on the transformative potential of AI in advancing materials science and biomedicine, as well as its ability to address previously intractable problems.</div></div>","PeriodicalId":279,"journal":{"name":"Colloids and Surfaces B: Biointerfaces","volume":"255 ","pages":"Article 114970"},"PeriodicalIF":5.4,"publicationDate":"2025-07-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144679498","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Boosting ferroptosis therapy by sorafenib and ML385 loaded CuFe2O4 nanozymes","authors":"Yanzhao Yin ,&nbsp;Junsheng Zhao ,&nbsp;Mengxiao Liu ,&nbsp;Ying Lu ,&nbsp;Jin Cao ,&nbsp;Rong Zhao ,&nbsp;Lin Wu ,&nbsp;Song Shen","doi":"10.1016/j.colsurfb.2025.114968","DOIUrl":"10.1016/j.colsurfb.2025.114968","url":null,"abstract":"<div><div>Ferroptosis as a promising therapeutic modality has been widely employed in cancer treatment. However, low efficacy of Fenton reaction and the presence of antioxidants, including glutathione (GSH) and glutathione peroxidase 4 (GPX4), weaken the ferroptosis anticancer effects. In this study, we synthesized copper ferrite (CuFe₂O₄) nanozymes loaded with sorafenib and ML385 to boost ROS generation and improve the ferroptosis effects. The CuFe₂O₄ nanozymes exhibited superior Fenton reaction catalytic activities due to the coexistence of iron and copper ions. While, sorafenib and ML385 inhibited the antioxidant effect by impeding the synthesis of GSH and the expression of NRF2, respectively. To prevent the CuFe₂O₄ nanozymes from being quickly cleared by the reticuloendothelial system (RES), the nanozymes were further coated with the macrophage membrane. What's more, the CuFe₂O₄ also demonstrated excellent photothermal effect. This nanoplatform exhibited superior tumor therapeutic efficacy due to its exceptional GSH depletion ability, high ROS generation efficiency, and excellent photothermal therapy (PTT) effect, presenting considerable potential for cancer treatment.</div></div>","PeriodicalId":279,"journal":{"name":"Colloids and Surfaces B: Biointerfaces","volume":"255 ","pages":"Article 114968"},"PeriodicalIF":5.4,"publicationDate":"2025-07-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144679500","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Porous microneedles delivering apoptotic body-camouflaged nanozymes promote diabetic wound repairment through combined pro-angiogenesis, anti-inflammation, and bacterial elimination","authors":"Yang Chen ,&nbsp;Zhenlong Mu ,&nbsp;Yunfeng Hu ,&nbsp;Hui Wang ,&nbsp;Jin Li","doi":"10.1016/j.colsurfb.2025.114966","DOIUrl":"10.1016/j.colsurfb.2025.114966","url":null,"abstract":"<div><div>Chronic refractory diabetic wound healing remains a significant clinical problem due to hyperglycaemia-induced complicated wound microenvironments characterized by serious vascular dysfunction, dysregulated inflammatory responses, excessive reactive oxygen species (ROS) production, and recurrent bacterial infections. In this study, we developed an engineered composite termed BMn@H-Abs by encapsulating endothelial cell-derived apoptotic bodies (H-Abs) with manganese dioxide nanozymes (BMn). This BMn@H-Abs composite was further integrated into a microneedle (MN) patch using porous gelatin methacryloyl (GelMA) as the needle matrix, forming BMn@H-Abs MNs capable of penetrating dense wound eschar to achieve localized and sustained therapeutic delivery. Within diabetic wounds, the encapsulated H-Abs with inherent tissue regeneration and immunomodulatory properties effectively enhanced angiogenesis by promoting endothelial cell migration and tubulogenesis, while regulating macrophages polarization toward pro-regenerative and anti-inflammatory M2 phenotypes. The insertion of BMn@H-Abs MNs further restored oxygen supply, scavenged ROS levels, and inhibited bacterial growth through the catalase-mimetic and superoxide dismutase-mimetic activities of BMn nanozymes, as well as Mn^2 + -mediated antibacterial effects. This study demonstrates a novel MN-based therapeutic strategy that not only effectively penetrates the physical barriers within wounds but also implements multi-targeted interventions addressing the etiology, offering a promising approach for diabetic wound treatment.</div></div>","PeriodicalId":279,"journal":{"name":"Colloids and Surfaces B: Biointerfaces","volume":"255 ","pages":"Article 114966"},"PeriodicalIF":5.4,"publicationDate":"2025-07-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144679499","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Cell-penetrating peptides-modified nanocarrier for efficient, batch and noninvasive embryonic transfection","authors":"Yundong Li ,&nbsp;Sheng Huang ,&nbsp;Yunsheng Zheng ,&nbsp;Jiancong Xie ,&nbsp;Shigui Jiang ,&nbsp;Song Jiang ,&nbsp;Jianhua Huang ,&nbsp;Lishi Yang ,&nbsp;Qibin Yang ,&nbsp;Jianzhi Shi ,&nbsp;Yangyang Ding ,&nbsp;Erchao Li ,&nbsp;Ziyang Cao ,&nbsp;Falin Zhou","doi":"10.1016/j.colsurfb.2025.114965","DOIUrl":"10.1016/j.colsurfb.2025.114965","url":null,"abstract":"<div><div>Although gene-editing breeding demonstrates significant potential in aquaculture, the field still lacks efficient gene delivery systems. Fish and crustacean embryos typically possess hard egg membranes or thick shells, making conventional microinjection inefficient and causing high mortality rates. As a result, there is an urgent need for a universal, highly efficient, and low-toxicity noninvasive delivery platform. In this study, we investigated the potential of high-efficiency, large-scale transfection using nanocarrier technology, renowned for its biocompatibility and efficient molecular payload encapsulation. Our research focused on developing a novel nanocarrier system, termed TNP, composed of a poly(ethylene glycol)-poly(lactide-co-glycolide) block copolymer (PEG-<em>b</em>-PLGA) and a cell-penetrating peptide (TAT, transactivator of transcription). The TNP nanocarrier enabled noninvasive delivery of genetic cargo, including eGFP-mRNA and CRISPR/Cas9 components, achieving high-throughput transfection in both shrimp and zebrafish embryos with successful transgene expression and targeted gene editing. We demonstrated that TNP efficiently traversed embryonic barriers, leveraging to facilitate high-payload gene delivery while maintaining minimal cytotoxicity and high embryo viability based on the transmembrane functionality of TAT peptide. These findings highlight the TNP has the potential to transform aquaculture genetic engineering, enabling trait enhancement such as disease resistance and accelerated growth. Furthermore, this study establishes a versatile and efficient gene-editing platform for aquatic species, addressing the critical demand for sustainable and ethically responsible biotechnological innovations in global seafood production.</div></div>","PeriodicalId":279,"journal":{"name":"Colloids and Surfaces B: Biointerfaces","volume":"255 ","pages":"Article 114965"},"PeriodicalIF":5.4,"publicationDate":"2025-07-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144687086","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}