Colloids and Surfaces B: Biointerfaces最新文献

{"title":"Dexamethasone-loaded chitosan-decorated PLGA nanoparticles: A step forward in attenuating the COVID-19 cytokine storm?","authors":"Mirsiane Pascoal Costa ,&nbsp;João Octavio Carneiro Abdu ,&nbsp;Maria Clara Machado Resende Guedes ,&nbsp;Michelle Alvares Sarcinelli ,&nbsp;Rodrigo Luiz Fabri ,&nbsp;Frederico Pittella ,&nbsp;Gilson Costa Macedo ,&nbsp;Fernanda Maria Pinto Vilela ,&nbsp;Helvécio Vinícius Antunes Rocha ,&nbsp;Guilherme Diniz Tavares","doi":"10.1016/j.colsurfb.2024.114359","DOIUrl":"10.1016/j.colsurfb.2024.114359","url":null,"abstract":"<div><div>This study aims to develop and characterize poly (lactic-co-glycolic acid) (PLGA) nanoparticles decorated with chitosan (CS) for the encapsulation of dexamethasone (DEX) (NP-DEX-CS), targeting improved efficacy in the treatment of severe acute respiratory syndrome (SARS) associated with COVID-19. The nanoparticles were systematically characterized for size, zeta potential (ZP), morphology, encapsulation efficiency, and in vitro drug release. Incorporation of CS resulted in significant modifications in the nanoparticles' physical properties, notably an increase in size (from 207.3 ± 6.7 nm to 264.4 ± 4.4 nm) and a shift in ZP to positive values (from −11.8 ±1.4 mV to +30.0 ± 1,6 mV). The NP-DEX-CS formulation achieved a high encapsulation efficiency (∼79 %) and a drug loading capacity of 6.53 ± 0.02 %.In addition, the in vitro release rate of DEX from NP-DEX-CS was lower compared to undecorated nanoparticles, with a reduction from approximately 64–37 % within 24 h. Microscopy analyses revealed a smoother surface on the CS-decorated nanoparticles. FTIR and XRD analyses confirmed successful chitosan coating and DEX encapsulation. The CS coating enhanced the tolerability of J774.A1 cells to the nanoparticles, particularly evident at the highest concentration (400ug/mL), resulting in a cell viability ≥70 %. Importantly, the NP-DEX-CS significantly reduced levels of nitric oxide and inflammatory cytokines (IL-1, IL-6, IL-12, and TNF-α). These findings suggest that CS-decorated PLGA nanoparticles hold promise as an effective dexamethasone delivery system for treating SARS related to COVID-19.</div></div>","PeriodicalId":279,"journal":{"name":"Colloids and Surfaces B: Biointerfaces","volume":"246 ","pages":"Article 114359"},"PeriodicalIF":5.4,"publicationDate":"2024-11-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142610806","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Advances in the application of carbon dots-based fluorescent probes in disease biomarker detection.","authors":"Haoqi Zhang, Qingmei Zhang, Naihui Li, Guoqing Yang, Zewei Cheng, Xiujuan Du, Lingxiang Sun, Wei Wang, Bing Li","doi":"10.1016/j.colsurfb.2024.114360","DOIUrl":"https://doi.org/10.1016/j.colsurfb.2024.114360","url":null,"abstract":"<p><p>Carbon dots (CDs), as an emerging nanomaterial, have shown tremendous potential in disease biomarker detection. CDs can selectively interact with different target molecules, enabling highly sensitive and specific detection of these biomolecules. Compared to traditional detection methods, CDs sensors offer advantages such as rapid response, high detection sensitivity, and low cost. In this review, we summarize the latest advances in the application of CDs fluorescence probes for the detection of disease biomarkers, including sensing mechanisms, and their applications in the selective detection of metal ions, amino acids, enzymes, proteins, other biomolecules, as well as bacteria and viruses. We discuss the current challenges and issues associated with the practical application of CDs-based fluorescent probes. Furthermore, we propose future directions for the development of CDs. We hope that this review will provide new insights for researchers in the field of disease biomarker detection.</p>","PeriodicalId":279,"journal":{"name":"Colloids and Surfaces B: Biointerfaces","volume":"245 ","pages":"114360"},"PeriodicalIF":5.4,"publicationDate":"2024-11-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142610801","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Synergistic catecholamine and coordination chemistry for enhanced bioactivity and secondary grafting activity of zirconia dental implants","authors":"Peng Gao ,&nbsp;Qihong Zhang ,&nbsp;Yingyue Sun ,&nbsp;Huan Cheng ,&nbsp;Shuyi Wu ,&nbsp;Yinyan Zhang ,&nbsp;Wen Si ,&nbsp;Haobo Sun ,&nbsp;Ningyao Sun ,&nbsp;Jing Yang ,&nbsp;Kaiyong Cai ,&nbsp;Lei Lu ,&nbsp;Jinsong Liu","doi":"10.1016/j.colsurfb.2024.114361","DOIUrl":"10.1016/j.colsurfb.2024.114361","url":null,"abstract":"<div><div>The inherent bioinertness of zirconia (ZrO₂) hinders its early bone integration, presenting a significant obstacle to its widespread use in dental implant technologies. Addressing this, we developed a surface coating leveraging the synergistic effects of catecholamine and coordination chemistry inspired by the mussel byssus cuticle. This coating, named PDPA@Sr, is enriched with strontium ions and amine groups, resulting from a simple immersion of polydopamine (PD)-coated ZrO₂ in an alkaline strontium chloride and poly(allylamine) (PA) solution. Compared to conventional mussel-inspired PD coatings, PDPA@Sr demonstrates enhanced aesthetic properties and mechanical stability. The continuous release of strontium ions from the coating significantly enhances osteogenesis, while the abundant surface amine groups offer notable antibacterial effects. More importantly, these amine groups also enable a variety of chemical modifications, including electrostatic adsorption, carbodiimide chemistry, Michael addition, Schiff base formation, and click chemistry, thus providing a multifaceted platform for the advanced surface modification of ZrO₂ implants.</div></div>","PeriodicalId":279,"journal":{"name":"Colloids and Surfaces B: Biointerfaces","volume":"246 ","pages":"Article 114361"},"PeriodicalIF":5.4,"publicationDate":"2024-11-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142610818","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Imparting insoluble-soluble property to Cyt c by immobilizing Cyt c in UCST-pH dual responsive polymer for highly sensitive detection of phenol.","authors":"Yuanyuan Wang, Weimin Guan, Yulin Yang, Huiling Lan, Yu Wang, Yun Wang, Juan Han, Lei Wang","doi":"10.1016/j.colsurfb.2024.114344","DOIUrl":"https://doi.org/10.1016/j.colsurfb.2024.114344","url":null,"abstract":"<p><p>Immobilization of enzymes in porous organic framework (POF) materials is popular strategy to stabilize enzymes. For such solid enzyme catalysis system, improving the catalytic efficiency is challenging due to the diffusion resistance from solid-liquid interface and inner pores. Here, UCST-pH dual responsive polymeric carrier (PEG-b-PAAm-b-P(GMA-co-AAc)) was synthesized to immobilize cytochrome c (Cyt c), which impart the reversibly insoluble-soluble property to the immobilized Cyt c. The PEG-b-PAAm-b-P(GMA-co-AAc) could serve as an insoluble-soluble matrix to fast and efficiently immobilize Cyt c via covalent attachment, achieving a remarkable 92 % loading efficiency within just 120 min. The obtained insoluble PEG-b-PAAm-b-P(GMA-co-AAc)-Cyt c micelles exhibited an improvement in thermal, pH stability and reusability. The completely soluble PEG-b-PAAm-b-P(GMA-co-AAc)-Cyt c conjugates accelerated substrate diffusion and then enhanced the catalytic efficiency. These excellent advantages led to low detection limit (1.99 μM), lower than the presently reported biosensors based on enzyme mimics in the colorimetric detection of phenol. This UCST-pH dual responsive window presents a new platform to efficiently control the immobilization and release of enzymes, which will achieve excellent stability and catalytic efficiency.</p>","PeriodicalId":279,"journal":{"name":"Colloids and Surfaces B: Biointerfaces","volume":"245 ","pages":"114344"},"PeriodicalIF":5.4,"publicationDate":"2024-10-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142610803","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Matrix matters: How extracellular substances shape biofilm structure and mechanical properties","authors":"Md Ibnul Hasan,&nbsp;Srijan Aggarwal","doi":"10.1016/j.colsurfb.2024.114341","DOIUrl":"10.1016/j.colsurfb.2024.114341","url":null,"abstract":"<div><div>Biofilms possess unique mechanical properties that are vital to their stability and function. Biofilms are made of extracellular polymeric substances (EPS) secreted by microorganisms and comprise polysaccharides, proteins, extracellular DNA (eDNA), and lipids. EPS is the primary contributor and driver of the biofilm structure and mechanical properties such as stiffness, cohesion, and adhesion. EPS enhances the elasticity and viscosity of biofilms, allowing them to withstand mechanical stresses, shear forces, and deformation. Therefore, biofilms are notoriously difficult to remove and can result in billions of dollars in losses for various industries due to their adverse effects, such as contamination, pressure loss, and corrosion. As a result, it is essential to comprehend the mechanical properties of biofilms to control or remove them in various scenarios. We undertook a fundamental study to determine the relationship between individual EPS components and biofilm mechanical properties. In this study, a CDC biofilm reactor was used to grow pure culture biofilms (<em>Staphylococcus epidermidis</em>) which were treated with six EPS modifier agents (Ca²⁺, Mg²⁺, periodic acid, protease K, lipase, and DNAase I) to modify or cleave specific EPS components. The mechanical properties (Young's Modulus) of treated biofilms were subsequently tested using atomic force microscopy (AFM), the biofilm EPS functional groups were measured via the Fourier transform infrared (FTIR) spectroscopy, and biofilm structural characteristics using confocal imaging. The FTIR results showed that EPS modifier agents successfully reduced their target EPS components. Similarly, the confocal microscopic analysis results showed that most of these modifier agents (except lipase) significantly reduced (P-value &lt;0.05) the biovolume and thickness of treated biofilms. Conversely, most of these modifier agents (except protease K) significantly increased (P-value &lt;0.05) the roughness coefficient of the biofilms. Finally, data from AFM showed that biofilm mechanical properties (Young’s modulus) significantly (P-value &lt;0.05) changed with their EPS composition. These results have significant ramifications for biofilm management and control in myriad scenarios.</div></div>","PeriodicalId":279,"journal":{"name":"Colloids and Surfaces B: Biointerfaces","volume":"246 ","pages":"Article 114341"},"PeriodicalIF":5.4,"publicationDate":"2024-10-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142610811","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Inhibition and Disaggregation Effect of Flavonoid-derived Carbonized Polymer Dots on Protein Amyloid Aggregation","authors":"Dexin Li,&nbsp;Sujuan Wang,&nbsp;Jiawei Dong,&nbsp;Jie Li,&nbsp;Xinnan Wang,&nbsp;Feng Liu,&nbsp;Xinwu Ba","doi":"10.1016/j.colsurfb.2024.113928","DOIUrl":"10.1016/j.colsurfb.2024.113928","url":null,"abstract":"<div><p>In this research, four water-insoluble flavonoid compounds were utilized and reacted with arginine to prepare four carbonized polymer dots with good water-solubility in a hydrothermal reactor. Structural characterization demonstrated that the prepared carbonized polymer dots were classic core-shell structure. Effect of the prepared carbonized polymer dots on protein amyloid aggregation was further investigated using hen egg white lysozyme and human lysozyme as model protein in aqueous solution. All of the prepared carbonized polymer dots could retard the amyloid aggregation of hen egg white lysozyme and human lysozyme in a dose-depended manner. All measurements displayed that the inhibition ratio of luteolin-derived carbonized polymer dots (CPDs-1) was higher than that of the other three carbonized polymer dots under the same dosage. This result may be interpreted by the highest content of phenolic hydroxyl groups on the periphery. The inhibition ratio of CPDs-1 on hen egg white lysozyme and human lysozyme reached 88% and 83% at the concentration of 0.5<!--> <!-->mg/mL, respectively. CPDs-1 also could disaggregate the formed mature amyloid fibrils into short aggregates.</p></div>","PeriodicalId":279,"journal":{"name":"Colloids and Surfaces B: Biointerfaces","volume":"238 ","pages":"Article 113928"},"PeriodicalIF":5.8,"publicationDate":"2024-04-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140767287","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"High throughput microfluidics-based synthesis of PEGylated liposomes for precise size control and efficient drug encapsulation","authors":"Shima Akar,&nbsp;Somayeh Fardindoost,&nbsp;Mina Hoorfar","doi":"10.1016/j.colsurfb.2024.113926","DOIUrl":"https://doi.org/10.1016/j.colsurfb.2024.113926","url":null,"abstract":"<div><p>The low scalability and reproducibility of existing synthesis methods have hindered the translation of liposome nanoparticles as carriers for targeted drug delivery from conventional laboratory techniques to mass production. To this end, in this study, we present a high-throughput microfluidics-based approach for the synthesis of PEGylated liposomes with a primary focus on achieving precise size control and efficient encapsulation of hydrophobic drug molecules. In this platform, liposomes were self-assembled through a controllable mixing of lipids (EYPC, cholesterol, and DSPE-PEG 2000) dissolved in ethanol and an aqueous solution. The key parameters, including the chip design, total flow rate, flow rate ratio, lipid concentrations, as well as variations in buffer (HEPES and NaCl) and solvent composition (commercial and reagent-grade ethanol) were explored in detail. Through comprehensive parametric studies, we gained valuable insights into the influence of these variables on the size distribution of liposomes and succeeded in producing highly reproducible liposomes ranging from approximately 60 nm (corresponding to small unilamellar vesicles) to 150 nm (representing large unilamellar vesicles), all while maintaining a polydispersity index (PDI) of less than 0.2. To assess the encapsulation efficiency of hydrophobic drug molecules, Nile red (NR) was employed as a surrogate. We meticulously examined the impact of NR concentration on the drug encapsulation process, resulting in up to 74% drug encapsulation efficiency within the PEGylated liposomes. This research offers crucial advances in liposome synthesis and drug delivery, providing a high-throughput, controllable method for PEGylated liposomes with potential in pharmaceutical and biomedical fields.</p></div>","PeriodicalId":279,"journal":{"name":"Colloids and Surfaces B: Biointerfaces","volume":"238 ","pages":"Article 113926"},"PeriodicalIF":5.8,"publicationDate":"2024-04-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140646719","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Structural and tribological studies on the interaction of porcine gastric mucin with non- and cationic-modified β-lactoglobulins","authors":"Hadi Asgharzadeh Shirazi ,&nbsp;Seunghwan Lee ,&nbsp;Saif Ullah ,&nbsp;Kristoffer Almdal","doi":"10.1016/j.colsurfb.2024.113924","DOIUrl":"https://doi.org/10.1016/j.colsurfb.2024.113924","url":null,"abstract":"<div><p>β-lactoglobulin (BLG) is the major whey protein with negative charges at neutral pH in aqueous media. Thus, the interaction with mucins, the major polyanionic component of mucus, is very weak due to the electrostatic repulsion between them. The present study postulates that cationization of BLG molecules may reverse the interaction characteristics between BLG and mucin from repulsive to associative. To this end, cationic-modified BLGs were prepared by grafting positively charged ethylenediamine (EDA) moieties into the negatively charged carboxyl groups on the aspartic and glutamic acid residues and compared with non-modified BLG upon mixing with porcine gastric mucin (PGM). To characterize the structural and conformational features of PGM, non/cationized BLGs, and their mixtures, various spectroscopic approaches, including zeta potential, dynamic light scattering (DLS), and circular dichroism (CD) spectroscopy were employed. Importantly, we have taken surface adsorption with optical waveguide lightmode spectroscopy (OWLS), and tribological properties with pin-on-disk tribometry at the sliding interface as the key approaches to determine the interaction nature between them as mixing PGM with polycations can lead to synergistic lubrication at the nonpolar substrate in neutral aqueous media as a result of an electrostatic association. All the spectroscopic studies and a substantial improvement in lubricity collectively supported a tenacious and associative interaction between PGM and cationized BLGs, but not between PGM and non-modified BLG. This study demonstrates a unique and successful approach to intensify the interaction between BLG and mucins, which is meaningful for a broad range of disciplines, including food science, macromolecular interactions, and biolubrication etc.</p></div>","PeriodicalId":279,"journal":{"name":"Colloids and Surfaces B: Biointerfaces","volume":"238 ","pages":"Article 113924"},"PeriodicalIF":5.8,"publicationDate":"2024-04-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140646697","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Hierarchical antibiotic delivery system based on calcium phosphate cement/montmorillonite-gentamicin sulfate with drug release pathways","authors":"Lei Chen ,&nbsp;Xiuying Lin ,&nbsp;Min Wei ,&nbsp;Bo Zhang ,&nbsp;Yani Sun ,&nbsp;Xi Chen ,&nbsp;Shitong Zhang ,&nbsp;Hao Zhang ,&nbsp;Jieyu Zhang ,&nbsp;Xiaojiao Yu ,&nbsp;Binghua Yao ,&nbsp;Kang Zhao ,&nbsp;Yufei Tang ,&nbsp;Quanchang Tan ,&nbsp;Zixiang Wu","doi":"10.1016/j.colsurfb.2024.113925","DOIUrl":"https://doi.org/10.1016/j.colsurfb.2024.113925","url":null,"abstract":"<div><p>Antibiotic-loaded calcium phosphate cement (CPC) has emerged as a promising biomaterial for drug delivery in orthopedics. However, there are problems such as the burst release of antibiotics, low cumulative release ratio, inappropriate release cycle, inferior mechanical strength, and poor anti-collapse properties. In this research, montmorillonite-gentamicin (MMT-GS) was fabricated by solution intercalation method and served as the drug release pathways in CPC to avoid burst release of GS, achieving promoted cumulative release ratios and a release cycle matched the time of inflammatory response. The results indicated that the highest cumulative release ratio and release concentration of GS in CPC/MMT-GS was 94.1 ± 2.8 % and 1183.05 μg/mL, and the release cycle was up to 504 h. In addition, the hierarchical GS delivery system was divided into three stages, and the kinetics followed the Korsmeyer-Peppas model, the zero-order model, and the diffusion-dissolution model, respectively. Meanwhile, the compressive strength of CPC/MMT-GS was up to 51.33 ± 3.62 MPa. Antibacterial results demonstrated that CPC/MMT-GS exhibited excellent <em>in vitro</em> long-lasting antibacterial properties to <em>E. coli</em> and <em>S. aureus</em>. Furthermore, CPC/MMT-GS promoted osteoblast proliferation and exhibited excellent <em>in vivo</em> histocompatibility. Therefore, CPC/MMT-GS has favorable application prospects in the treatment of bone defects with bacterial infections and inflammatory reactions.</p></div>","PeriodicalId":279,"journal":{"name":"Colloids and Surfaces B: Biointerfaces","volume":"238 ","pages":"Article 113925"},"PeriodicalIF":5.8,"publicationDate":"2024-04-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140638156","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Synthesis of calix (4) resorcinarene based amphiphilic macrocycle as an efficient nanocarrier for Amphotericin-B to enhance its oral bioavailability","authors":"Imdad Ali ,&nbsp;Amjad Ali ,&nbsp;Li Guo ,&nbsp;Samiullah Burki ,&nbsp;Jawad Ur Rehman ,&nbsp;Mahmood Fazal ,&nbsp;Naushad Ahmad ,&nbsp;Sarzamin Khan ,&nbsp;Carlos A.T. Toloza ,&nbsp;Muhammad Raza Shah","doi":"10.1016/j.colsurfb.2024.113918","DOIUrl":"https://doi.org/10.1016/j.colsurfb.2024.113918","url":null,"abstract":"<div><p>The supramolecular-based macrocyclic amphiphiles have fascinating attention and find extensive utilization in the pharmaceutical industry for efficient drug delivery. In this study, we designed and synthesized a new supramolecular amphiphilic macrocycle to serve as an efficient nanocarrier, achieved by treating 4-hydroxybenzaldehyde with 1-bromotetradecane. The derivatized product was subsequently treated with resorcinol to cyclize, resulting in the formation of a calix(4)-resorcinarene-based supramolecular amphiphilic macrocycle. The synthesized macrocycle and intermediate products were characterized using mass spectrometry, IR, and ¹H NMR spectroscopic techniques. The amphotericin-B (Amph-B)-loaded and unloaded amphiphiles were screened for biocompatibility studies, vesicle formation, particle shape, size, surface charge, drug entrapment, <em>in-vitro</em> release profile, and stability through atomic force microscopy (AFM), Zetasizer, HPLC, and FT-IR. Amph-B -loaded macrocycle-based niosomal vesicles were investigated for <em>in-vivo</em> bioavailability in rabbits. The synthesized macrocycle exhibited no cytotoxicity against normal mouse fibroblast cells and was found to be hemocompatible and safe in mice following an acute toxicity study. The drug-loaded macrocycle-based vesicles appeared spherical, nano-sized, and homogeneous in size, with a notable negative surface charge. The vesicles remained stable after 30 days of storage. The results of Amph-B oral bioavailability and pharmacokinetics revealed that the newly tailored niosomal formulation enhanced drug solubility, protected drug degradation at gastric pH, facilitated sustained drug release at the specific target site, and delayed plasma drug clearance. Incorporating such advanced niosomal formulations in the field of drug delivery systems has the potential to revolutionize therapeutic outcomes and improve the quality of patient well-being.</p></div>","PeriodicalId":279,"journal":{"name":"Colloids and Surfaces B: Biointerfaces","volume":"238 ","pages":"Article 113918"},"PeriodicalIF":5.8,"publicationDate":"2024-04-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140646695","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}