Nano-selenium alleviated immunoresponse, apoptosis and oxidative stress in Leydig cells of yak

IF 5.4 2区医学 Q1 BIOPHYSICS

Colloids and Surfaces B: Biointerfaces Pub Date : 2025-04-09 DOI:10.1016/j.colsurfb.2025.114684

Hao Li , Yujun Tang , Hui Wang , Xinyue Liu , Yutian Zeng , Run Zhang , Cuiting Yang , Arab Khan , Bing Wu , Xianxiang Wang , Ming Zhang

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Abstract

Nano-selenium(SENP) plays a crucial role in maintaining cellular redox homeostasis and serves as an antioxidant in cell culture medium. This study investigated the cytoprotective effects of SENP against lipopolysaccharide (LPS)-induced toxicity in yak Leydig cells. In this research, in vitro cultured Leydig cells were exposed to LPS to simulate Gram-negative bacterial infection. Following LPS induction, the cell apoptosis rate reached 28 %, with significant increases in inflammation and oxidative stress markers including IL-6, IL-8, MDA, and ROS. Concurrently, testosterone concentration decreased by nearly 60 %. Subsequently, SENP was introduced into the culture medium. We then evaluated apoptosis, oxidative stress, immune response, and testosterone concentration in Leydig cells. The results demonstrated that SENP effectively protected Leydig cells from LPS-induced damage.

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纳米硒可减轻牦牛间质细胞的免疫应答、凋亡和氧化应激

纳米硒（SENP）在维持细胞氧化还原平衡方面发挥着重要作用，是细胞培养基中的一种抗氧化剂。本研究探讨了 SENP 对脂多糖（LPS）诱导的牦牛 Leydig 细胞毒性的细胞保护作用。在这项研究中，体外培养的 Leydig 细胞暴露于 LPS，以模拟革兰氏阴性细菌感染。LPS诱导后，细胞凋亡率达到28%，炎症和氧化应激指标（包括IL-6、IL-8、MDA和ROS）显著增加。同时，睾酮浓度下降了近 60%。随后，在培养基中引入 SENP。然后，我们评估了凋亡、氧化应激、免疫反应和睾酮浓度。结果表明，SENP 能有效保护 Leydig 细胞免受 LPS 诱导的损伤。

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来源期刊

Colloids and Surfaces B: Biointerfaces 生物-材料科学：生物材料

CiteScore

11.10

自引率

3.40%

发文量

730

审稿时长

42 days

期刊介绍： Colloids and Surfaces B: Biointerfaces is an international journal devoted to fundamental and applied research on colloid and interfacial phenomena in relation to systems of biological origin, having particular relevance to the medical, pharmaceutical, biotechnological, food and cosmetic fields. Submissions that: (1) deal solely with biological phenomena and do not describe the physico-chemical or colloid-chemical background and/or mechanism of the phenomena, and (2) deal solely with colloid/interfacial phenomena and do not have appropriate biological content or relevance, are outside the scope of the journal and will not be considered for publication. The journal publishes regular research papers, reviews, short communications and invited perspective articles, called BioInterface Perspectives. The BioInterface Perspective provide researchers the opportunity to review their own work, as well as provide insight into the work of others that inspired and influenced the author. Regular articles should have a maximum total length of 6,000 words. In addition, a (combined) maximum of 8 normal-sized figures and/or tables is allowed (so for instance 3 tables and 5 figures). For multiple-panel figures each set of two panels equates to one figure. Short communications should not exceed half of the above. It is required to give on the article cover page a short statistical summary of the article listing the total number of words and tables/figures.