化学•材料最新文献

{"title":"CRISPR/Cas13a-driven lateral flow assay for preamplification-free and ultrasensitive miRNA-21 detection.","authors":"Yan Zhu, Junwei Lai, Xinyao Yang, Shuqing Wang, Dayong Gu, Yan Huang, Yizhen Liu, Conghui Liu","doi":"10.1016/j.bios.2025.117850","DOIUrl":"10.1016/j.bios.2025.117850","url":null,"abstract":"<p><p>Developing a preamplification-free and sensitive clustered regularly interspaced short palindromic repeats (CRISPR)-based method is significant but still extremely challenging for microRNA (miRNA) detection. Here we present a combination of a CRISPR/Cas13a-based reaction with a lateral flow biosensor, which enables the quantitative and colorimetric readout of preamplification-free miRNA detection at room temperature. In this work, the reaction principle and the structure of the lateral flow strip are well-designed to achieve surface-enhanced Raman scattering (SERS)/colorimetric dual-signal \"turn-on\" response of target miRNA. The CRISPR/Cas13a Reporter is engineered with a DNA-RNA splicing structure to generate DNA cleavage products and reduce nonspecific collateral cleavage. Without the need for nucleic acid preamplification strategy, the developed CRISPR/Cas13a-driven lateral flow biosensor enables the microRNA-21 (miR-21) detection at room temperature with a readout time of 10 min and a total process time of less than 45 min, achieving an impressive limit of detection of 8.96 aM by SERS and 1 fM by visualization, respectively. Moreover, the platform demonstrated excellent recovery rates in spiked human serum samples. The proposed CRISPR/Cas13a-driven, dual-signal \"turn-on\"-responded lateral flow platform has the potential to simultaneously meet the requirements of convenient point-of-care visualization detection and more accurate and sensitive SERS detection of miR-21, offering a cost-effective, rapid, and reliable tool for early cancer diagnosis.</p>","PeriodicalId":259,"journal":{"name":"Biosensors and Bioelectronics","volume":"288 ","pages":"117850"},"PeriodicalIF":10.5,"publicationDate":"2025-11-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144803043","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"SERS-based miniaturized biosensors for alkaline phosphatase detection: Towards intelligent, real-time diagnostics in precision medicine.","authors":"Ajitesh Dhal, Ana Elena Aviña, Cheng-Jen Chang, Chang-I Chen, Tzu Sen Yang","doi":"10.1016/j.bios.2025.117793","DOIUrl":"10.1016/j.bios.2025.117793","url":null,"abstract":"<p><p>Alkaline phosphatase (ALP) is a clinically important hydrolase enzyme and a valuable biomarker for hepatobiliary diseases, metabolic bone disorders, and certain malignancies. Raman-based miniaturized sensors, particularly those employing surface-enhanced Raman scattering (SERS), have enabled ultrasensitive and selective ALP detection at femtomolar to picomolar levels in complex biological samples. This narrative review critically examines recent advances in SERS-enabled ALP sensors, highlighting hotspot engineering, nanozyme-assisted signal amplification, and microfluidic integration to achieve high-throughput, low-volume assays. It also explores the incorporation of artificial intelligence algorithms for real-time spectral interpretation and discusses the potential for integrating these systems with fifth and sixth generation (5G/6G) wireless networks for rapid, cloud-based diagnostics. In addition, this review outlines current challenges, including substrate reproducibility and standardization issues, and proposes strategies to enhance clinical translation. Collectively, these developments are transforming ALP sensing by enabling decentralized, intelligent, and personalized diagnostic platforms, which hold promise for advancing precision healthcare and improving patient outcomes.</p>","PeriodicalId":259,"journal":{"name":"Biosensors and Bioelectronics","volume":"288 ","pages":"117793"},"PeriodicalIF":10.5,"publicationDate":"2025-11-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144803044","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Corrigendum to \"A CRISPR-Cas12a-mediated dual-mode luminescence and colorimetric nucleic acid biosensing platform based on upconversion nanozyme\" [Biosens. Bioelectron. 270 (2025) 116963].","authors":"Jiaxiang Yan, Bohan Yin, Qin Zhang, Chuanqi Li, Jiareng Chen, Yingying Huang, Jianhua Hao, Changqing Yi, Yu Zhang, Siu Hong Dexter Wong, Mo Yang","doi":"10.1016/j.bios.2025.117738","DOIUrl":"10.1016/j.bios.2025.117738","url":null,"abstract":"","PeriodicalId":259,"journal":{"name":"Biosensors and Bioelectronics","volume":" ","pages":"117738"},"PeriodicalIF":10.7,"publicationDate":"2025-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144566883","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Significant improvements in targeted UHPLC-ESI-MS/MS analysis of the reactive aldehydes 4-hydroxy-2(E)-nonenal and 4-hydroxy-2(E)-hexenal and application to rat serum.","authors":"S Chevolleau, C Orlandi, L Mervant, R Vuillaume, I Jouanin, N Naud, F Pierre, F Gueraud, L Debrauwer","doi":"10.1016/j.jchromb.2025.124747","DOIUrl":"10.1016/j.jchromb.2025.124747","url":null,"abstract":"<p><p>The elevated risk of colorectal cancer (CRC) induced by red or processed meat rich diets is now established. Those haem‑iron rich diets induce luminal lipid peroxidation, one of the most recognised hypotheses explaining CRC promotion. Due to their known toxic properties, quantification of reactive aldehydes such as 4-hydroxy-2(E)-nonenal (HNE) and 4-hydroxy-2(E)-hexenal (HHE) as lipid peroxidation end-products in biological fluids is of upmost importance. Following previous works on faecal waters, an UHPLC-ESI-MS/MS method has been developed and validated for HNE and HHE quantification in rat serum, using deuterated internal standards (ISs). After protein precipitation (PP) and solid phase extraction (SPE), LC-ESI-MS/MS analysis was achieved by MRM. The use of a brominated derivatisation reagent allowed using the bromine isotopes for selective detection of both HNE and HHE based on diagnostic transitions. This new method was validated according to the European Medicines Agency (EMA) guidelines. Our method proved to efficiently determine HNE and HHE serum concentrations with the required sensitivity (nM range) in serum of rats fed diets rich or not in red meat and different fatty acid compositions.</p>","PeriodicalId":348,"journal":{"name":"Journal of Chromatography B","volume":"1265 ","pages":"124747"},"PeriodicalIF":2.8,"publicationDate":"2025-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144811470","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Corrigendum to \"Development of a universal one-pot CRISPR assay based on multifunctional tagged primer eliminating unstable crRNA input and PAM dependency for point-of-care detection of bacterial infections\" [Biosens. Bioelectron. 287 (2025) 117718].","authors":"Xiaofan Zhu, Jingran Jiao, Qun Ni, Li Yao, Yule Zhang, Kaiyong Liu, Lin Huang, Qingli Bo, Panzhu Qin","doi":"10.1016/j.bios.2025.117742","DOIUrl":"10.1016/j.bios.2025.117742","url":null,"abstract":"","PeriodicalId":259,"journal":{"name":"Biosensors and Bioelectronics","volume":" ","pages":"117742"},"PeriodicalIF":10.7,"publicationDate":"2025-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144564200","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Integrating network pharmacology and metabolomics to elucidate the mechanism of action of Yangluan formula for treating of diminished ovarian reserve.","authors":"Yang Wang, Panwei Hu, Hua Yan, Yuanyuan Wu, Ping Yin, Dongyi Shen, Xiaole Zhang, Cong Qi, Qinhua Zhang","doi":"10.1016/j.jchromb.2025.124749","DOIUrl":"10.1016/j.jchromb.2025.124749","url":null,"abstract":"<p><strong>Objectives: </strong>The Yangluan Formula (YLF) influences the outcomes of in vitro fertilization-embryo transfer (IVF-ET) in infertile women with diminished ovarian reserve (DOR); however, the potential mechanisms by which YLF ameliorates DOR have not yet been elucidated. The aim of this study was to examine the effects of YLF on IVF-ET outcomes in infertile women with DOR, and to elucidate the potential mechanisms by which YLF addresses DOR.</p><p><strong>Methods: </strong>Non-targeted metabolomics studies were conducted on follicular fluid specimens procured from individuals with DOR treated with or without YLF, and from patients with normal ovarian reserve who underwent IVF-ET treatment. Distinct metabolites were identified using untargeted metabolomics, and MetaboAnalyst was used to examine metabolic pathways. After applying network pharmacology (NP), the target of YLF acting on DOR was determined. Cytoscape software was used to develop compound-reaction-enzyme-gene networks, and molecular docking (MD) simulations were conducted to confirm the link between YLF and crucial targets.</p><p><strong>Results: </strong>Patients with DOR showed a notable reduction in the number of oocytes retrieved, incidence of 2PN fertilization, and number of cleaved embryos (P < 0.001). Additionally, the DOR cohort exhibited a markedly reduced quantity of high-quality embryos on day 3 compared to the CON cohort (P < 0.005). In contrast to the DOR cohort, the YLF cohort exhibited notably superior outcomes in terms of 2PN fertilization rates, cleavage-stage embryo development, and the number of high-grade embryos on day 3 (P < 0.05). The proportion of 2PN fertilization observed in YLF subjects substantially exceeded that in DOR individuals (81.2 % vs. 64.3 %, P < 0.05). Combined analysis of metabolomics and NP, focusing on five key targets for the action of YLF (monoamine oxidase A, monoamine oxidase B, myeloperoxidase, xanthine dehydrogenase, and phosphodiesterase 3A), four key metabolites (pelargonic acid, 1-(5-Phospho-D-ribosyl)-5-amino-4-imidazolecarboxylate, isokobusone, 5-O-(1-Carboxyvinyl)-3-phosphoshikimate), and two related pathways (glycine, serine, alanine, and threonine metabolism).</p><p><strong>Conclusion: </strong>We elucidated the mode of action of YLF in DOR treatment by integrating metabolomics and NP. YLF can effectively improve IVF outcomes in patients with DOR. This study provides new perspectives on the mechanism by which YLF improves ovarian function.</p>","PeriodicalId":348,"journal":{"name":"Journal of Chromatography B","volume":"1265 ","pages":"124749"},"PeriodicalIF":2.8,"publicationDate":"2025-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144797780","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Ferrocene derivatives constructed Prussian blue analogs for colorimetric detection of hydrogen peroxide and glucose.","authors":"Yue Tong, Qian Liu, Dan Yang, Chunze Zhang, Yi Xiao, Mengying Shi, Meng Meng, Huixia Di, Hongmei Gao, Rimo Xi, Yongmei Yin","doi":"10.1016/j.bios.2025.117714","DOIUrl":"10.1016/j.bios.2025.117714","url":null,"abstract":"<p><p>This study reports the rational design of ferrocene-derived Prussian blue analogs (PBAs) as a new class of nanozymes with enhanced catalytic properties. Capitalizing on its high peroxidase-like activity, we developed a colorimetric assay for detecting hydrogen peroxide (H₂O₂) and glucose, which exhibited high stability, sensitivity, and accuracy in biological samples such as serum. Furthermore, by leveraging the reversible structural dynamics of Fc(COOH)₂-PBA, we engineered a dual-mode colorimetric assay for visual quantitation of H₂O₂ and glucose in complex samples, including cells, tissues, and cerebrospinal fluid. The distinct colorimetric response of this system demonstrates promising potential for point-of-care testing applications. Our findings provide mechanistic insights into the structure-activity relationships of nanozymes and will facilitate the development of application-targeted nanozymes for disease diagnostics.</p>","PeriodicalId":259,"journal":{"name":"Biosensors and Bioelectronics","volume":" ","pages":"117714"},"PeriodicalIF":10.7,"publicationDate":"2025-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144493209","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Nuclear Quantum Effects in OH*/OD* Formation Kinetics on Pd(332): A Ring-Polymer Molecular Dynamics Study.","authors":"Liang Zhang,Chen Li,Michael Schwarzer,Dmitriy Borodin,Alec M Wodtke,Hua Guo","doi":"10.1021/acs.jpclett.5c02891","DOIUrl":"https://doi.org/10.1021/acs.jpclett.5c02891","url":null,"abstract":"A complete understanding of kinetics of heterogeneous catalysis requires accurate calculations of reaction rates for surface reactions, which is coverage-dependent. In this letter, a ring-polymer molecular dynamics investigation is carried out for a key step in the Pd-catalyzed hydrogen oxidation, namely, the formation of OH from adsorbed H and O species at both high and low coverages on Pd(332). It is found that the calculated rate coefficients agree with the recently reported experimental data. In addition, significant nuclear quantum effects are found even at relatively high temperatures.","PeriodicalId":62,"journal":{"name":"The Journal of Physical Chemistry Letters","volume":"41 1","pages":"11175-11182"},"PeriodicalIF":6.475,"publicationDate":"2025-10-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145319100","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A Quantitative Environmental Risk Assessment for Microplastics in Sewage Sludge Applied to Land.","authors":"Paul Boisseaux,Marie Laure Delignette-Muller,Tamara Galloway","doi":"10.1021/acs.est.5c08026","DOIUrl":"https://doi.org/10.1021/acs.est.5c08026","url":null,"abstract":"The application of sewage sludge to land delivers high levels of microplastics (MPs), contributing to soil contamination and chronic effects on soil biota. Despite this, the quantitative chronic risk assessment of MPs in sludge-amended soils (SAS) to soil biota has not been thoroughly addressed, and none has been done directly on sludge samples. Here, we combined environmental exposure and species sensitivity distributions, built on published data, with two-dimensional Monte Carlo simulations to characterize the risk from MPs in sludge samples and in SAS, testing worst-case and realistic case scenarios of sludge behavior in soil. Contamination with MPs frequently exceeded the concentration likely to affect more than 5% of the species. Risk characterization showed that the worst-case MP scenario affects 39% of species, while the realistic scenario affects 15-18% of soil species, implying that the current state of sludge application fails to protect 95% of soil biota. The percentage of species affected by MPs approximately doubled in SAS. Our conservative results suggest that regulatory limits on MPs in sludge being applied to land should be urgently considered.","PeriodicalId":36,"journal":{"name":"环境科学与技术","volume":"15 1","pages":""},"PeriodicalIF":9.028,"publicationDate":"2025-10-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145319296","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Electrochemical Synthesis of 3,5-Disubstituted Pyrazoles from Ynone Hydrazones via Diselenide-Catalyzed Cyclization Strategy.","authors":"Zhouting Zeng,Feng Zhao,Huimei Zhong,Zi Yang,Huaixin Wei,Songwen Ji,Mingming Yu,Jinhui Cai","doi":"10.1021/acs.joc.5c02108","DOIUrl":"https://doi.org/10.1021/acs.joc.5c02108","url":null,"abstract":"Given the existing limitations of traditional methods for the synthesis of pyrazole derivatives, developing new, mild, and efficient approach toward pyrazole skeletons is necessary. Herein, we report an electrochemically driven diselenide-catalyzed cyclization of 1,3-disubstituted prop-2-yn-1-one hydrazones that realize the rapid synthesis of 3,5-disubstituted pyrazoles under metal-free, oxidant-free, and base-free conditions. The practical utility of this method is underscored by its wide substrate scope, high tolerance toward various functional groups, high efficiency, mild conditions, gram-scale synthesis, and diverse derivatizations. Furthermore, the preliminary mechanistic experiments indicate that this reaction possibly proceeds via a cationic pathway.","PeriodicalId":57,"journal":{"name":"Journal of Organic Chemistry","volume":"95 1","pages":""},"PeriodicalIF":4.354,"publicationDate":"2025-10-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145319174","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}