化学•材料最新文献

{"title":"Trident-inspired fucoidan-based armor-piercing microcapsule for programmed acute pulmonary embolism treatment.","authors":"Ning Yang, Weikun Li, Zhicheng Qian, Xin Tan, Zonghao Liu, Feiling Feng, Ling Liu, Liqin Ge","doi":"10.1016/j.colsurfb.2024.114323","DOIUrl":"10.1016/j.colsurfb.2024.114323","url":null,"abstract":"<p><p>Pulmonary embolism remains the third leading cause of human mortality after malignant tumors and myocardial infarction. Commonly available thrombolytic therapeutic agents suffer from the limitations of very short half-life, inadequate targeting, limited clot penetration, and a propensity for severe bleeding. Inspired by the trident, we developed the armor-piercing microcapsule (MC), fucoidan-urokinase-S-nitrosoglutathione-polydopamine@MC (FUGP@MC), which exhibited a triple combination of photothermal, mechanical and pharmacological thrombolysis for the therapeutic treatment of acute pulmonary embolism (APE). Briefly, the outermost fucoidan layer was utilized for targeting to the APE area. Programmed APE treatment was triggered by near-infrared (NIR) light irradiation. Photothermal thrombolytic therapy was carried out by photothermal conversion of polydopamine. The photothermal conversion broke the S-nitroso bond in S-nitrosoglutathione (GSNO) and produced large amounts of nitric oxide (NO) for mechanical thrombolysis, which subsequently disrupted the interfacial structure of microcapsule to stimulate the release of the urokinase (UK), leading to a triple synergistic thrombolytic effect. The results demonstrated that the embolization residual rate of FUGP@MC (contained ≈ 1452.5 IU/kg UK) group was significantly lower than that of UK (10,000 IU/kg) group (6.35 % VS 16.78 %). Remarkably, FUGP@MC demonstrated a reliable in vivo biosafety proficiency. In summary, trident-inspired armor-piercing microcapsule FUGP@MC reveals a potential avenue for advancing pulmonary embolism therapeutics and promises to be a safer alternative candidate to current drug approaches.</p>","PeriodicalId":279,"journal":{"name":"Colloids and Surfaces B: Biointerfaces","volume":"245 ","pages":"114323"},"PeriodicalIF":5.4,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142491925","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Translocation mechanism of anticancer drugs through membrane with the assistance of graphene quantum dot.","authors":"Luxi Weng, Hao Ren, Ruru Xu, Jiahao Xu, Jun Lin, Jia-Wei Shen, Yongke Zheng","doi":"10.1016/j.colsurfb.2024.114340","DOIUrl":"10.1016/j.colsurfb.2024.114340","url":null,"abstract":"<p><p>In recent years, as a new type of quasi-zero-dimensional nanomaterials, graphene quantum dots (GQDs) have shown excellent performance in advanced drug targeted delivery and controlled release. In this work, the delivery process of model drugs translocating into POPC lipid membrane with the assistance of GQDs was investigated via molecular dynamics (MD) simulation. Our simulation results demonstrated that a single doxorubicin (DOX) or deoxyadenine (DA) molecule is difficult to penetrate into the cell membrane. GQD7 could form sandwich-like structure with DOX and assist DOX to enter into the POPC membrane. However, due to the weak interaction with DA, both GQD7 and GQD19 can not assist DA translocating the POPC membrane in the limited MD simulation time. The drug delivery process for DOX could be divided into two steps: 1. GQDs and DOX aggregated into a cluster; 2. the aggregates enter into the POPC membrane. In all our simulation systems, if GQDs loaded with model drugs and entered the cell membrane, it had little effect on the cell membrane structure, and the cell membrane could maintain high integrity and stability. These results may promote the molecular design and application of GQD-based drug delivery systems.</p>","PeriodicalId":279,"journal":{"name":"Colloids and Surfaces B: Biointerfaces","volume":"245 ","pages":"114340"},"PeriodicalIF":5.4,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142542497","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Bioactive dextran-based scaffolds from emulsion templates co-stabilized by poly(lactic-co-glycolic acid) nanocarriers.","authors":"Maude Ducrocq, Arianna Rinaldi, Boris Halgand, Joëlle Veziers, Pierre Guihard, Frank Boury, Antoine Debuigne","doi":"10.1016/j.colsurfb.2024.114342","DOIUrl":"10.1016/j.colsurfb.2024.114342","url":null,"abstract":"<p><p>Porous polymer scaffolds are widely investigated as temporary implants in regenerative medicine to repair damaged tissues. While biocompatibility, degradability, mechanical properties comparable to the native tissues and controlled porosity are prerequisite for these scaffolds, their loading with pharmaceutical or biological active ingredients such as growth factors, in particular proteins, opens up new perspective for tissue engineering applications. This implies the development of scaffold loading strategies that minimize the risk of protein denaturation and allow to control their release profile. This work reports on a straightforward method for preparing bioactive dextran-based scaffolds from high internal phase emulsion (HIPE) templates containing poly(lactic-co-glycolic acid) (PLGA) nanoparticles (NPs) serving both as co-stabilizers for the emulsion and nanocarriers for drug or therapeutic protein models. Scaffold synthesis are achieved by photocuring of methacrylated dextran located in the external phase of a HIPE stabilized by the NPs in combination or not with a non-ionic surfactant. Fluorescent labelling of the NPs highlights their integration in the scaffold. The introduction of NPs, and even more so when combined with a surfactant, increases the stability and mechanical properties of the scaffolds. Cell viability tests demonstrate the non-toxic nature of these NPs-loaded scaffolds. The study of the release of a model protein from the scaffold, namely lysozyme, shows that its encapsulation in nanoparticles decreases the release rate and provides additional control over the release profile.</p>","PeriodicalId":279,"journal":{"name":"Colloids and Surfaces B: Biointerfaces","volume":"245 ","pages":"114342"},"PeriodicalIF":5.4,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142563519","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Synergistic effect of 2D covalent organic frameworks confined 0D carbon quantum dots film: Toward molecularly imprinted cathodic photoelectrochemical platform for detection of tetracycline.","authors":"Mengge Zhang, Xiuxiu Dong, Guanya Ji, Jing Wang, Tao Wang, Qian Liu, Qijian Niu","doi":"10.1016/j.bios.2024.116870","DOIUrl":"10.1016/j.bios.2024.116870","url":null,"abstract":"<p><p>The development of high photoactive cathode materials combined with the formation of a stable interface are considered important factors for the selective and sensitive photoelectrochemical (PEC) detection of tetracycline (TC). Along these lines, in this work, a novel type II heterostructure composed of two-dimensional (2D) covalent organic frameworks confined to zero-dimensional (0D) carbon quantum dots (CDs/COFs) film was successfully synthesized using the rapid in-situ polymerization method at room temperature. The PEC signal of CDs/COFs was significantly amplified by improving the light absorption and electron transfer capabilities. Furthermore, a cathodic molecularly imprinted PEC sensor (MIP-PEC) for the detection of TC was constructed through fast in-situ Ultraviolet (UV) photopolymerization on the electrode. Finally, a \"turn-off\" PEC cathodic signal was achieved based on the selective recognition of the imprinted cavity and the mechanism of steric hindrance increase. Under optimal conditions, the proposed sensor demonstrated a wide linear relationship with TC in the concentration range of 5.00 × 10^-12-1.00 × 10^-5 M, with a detection limit as low as 6.00 × 10^-13 M. Meanwhile, excellent stability, selectivity, reproducibility, and applicability in real river samples was recorded. Our work provides an effective and rapid in situ construction method for fabricating highly photoactive cathode heterojunctions and uniform stable selective MIP-PEC sensing interfaces, yielding accurate antibiotics detection in the environment.</p>","PeriodicalId":259,"journal":{"name":"Biosensors and Bioelectronics","volume":"267 ","pages":"116870"},"PeriodicalIF":10.7,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142491919","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Reactive oxygen species-responsive coating based on Ebselen: Antioxidation, pro-endothelialization and anti-hyperplasia for surface modification of cardiovascular stent.","authors":"Xinyi Chen, Rui Wang, Yuhua Du, Yonghong Mou, Yan Gong, Xueyu Zhao, Wenzhong Li, Xin Li, Jin Wang, Yuancong Zhao","doi":"10.1016/j.colsurfb.2024.114314","DOIUrl":"10.1016/j.colsurfb.2024.114314","url":null,"abstract":"<p><p>Atherosclerosis is often accompanied by inflammation and oxidative stress. Excessive reactive oxygen species (ROS) can damage the vascular endothelium, leading to endothelial dysfunction and reduced nitric oxide (NO) bioavailability. Further accumulation of ROS contributes to vascular cell damage, lipid peroxidation, and extracellular matrix deposition. Thus, clearing excess ROS and reshaping the oxidative microenvironment is essential for treating atherosclerosis (AS). In this study, Ebselen, which mimics glutathione peroxidase and possesses redox capabilities, was successfully synthesized. Subsequently, a multifunctional coating was designed using a combination of Ebselen and poly (trimethylene carbonate) (PTMC), capable of protecting cells from ROS-induced damage, promoting vascular endothelialization, and exhibiting anti-proliferative properties. The Ebselen-loaded coating effectively scavenges free radicals (with an elimination rate of 89 %), catalytically releases NO (0.96 × 10⁻¹⁰ to 1.26 × 10⁻¹⁰ mol/cm²/min), and sustainably delivers Ebselen to the lesion site through a redox cycle. Notably, this coating shows excellent hemocompatibility and cytocompatibility. Subcutaneous implantation results indicated that the fibrous capsule thickness of PTMC10 was the lowest, at just 47.7 % of that of PTMC. Therefore, the Ebselen-loaded coating presents promising applications in cardiovascular stents.</p>","PeriodicalId":279,"journal":{"name":"Colloids and Surfaces B: Biointerfaces","volume":"245 ","pages":"114314"},"PeriodicalIF":5.4,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142454513","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Hierarchy-constructed superhydrophobic and transparent coating modified intraocular lens by layer-by-layer self-assembly for glistening reduction and antiadhesion.","authors":"Sihao Liu, Xia Zhao, Yuemei Han, Quankui Lin","doi":"10.1016/j.colsurfb.2024.114333","DOIUrl":"10.1016/j.colsurfb.2024.114333","url":null,"abstract":"<p><p>Intraocular lens (IOL) implantation surgery is the most effective treatment for cataract. However, glistening formed by the incoming liquid microvacuoles can significantly damage postoperative visual quality after prolonged implantation, for which there is still lack of effective clinical treatment. In this study, inspired by the amazing water-repellency of natural superhydrophobic surface, a functionalized IOL material modified with the superhydrophobic and transparent coating was prepared using layer-by-layer electrostatic self-assembly technique combined with fluorination. After the alternate deposition of multiple cationic/anionic polyelectrolytes and silica nanoparticles of varying sizes on IOL materials, the constructed multilayered films with special surface roughness were further fluorinated to reduce surface energy. In addition to its excellent superhydrophobicity and transparency, this multilayered coating could efficiently eliminate the glistening formation of IOL under accelerated condition in vitro. Furthermore, the in vitro experiments with water droplets, cells, and bacteria suggested the superior antiadhesion property of such coating modified materials. The biocompatibility evaluation, both in vitro and in vivo, demonstrated the great biocompatibility of the materials modified with superhydrophobic and transparent coating. Therefore, this multilayered coating with excellent superhydrophobic and transparent characteristics can provide an available approach aiming at anti-glistening and antiadhesion of IOL materials. Advances in the fabrication process of surface coating with specific functions will enhance the practical application and clinical success of modified IOLs.</p>","PeriodicalId":279,"journal":{"name":"Colloids and Surfaces B: Biointerfaces","volume":"245 ","pages":"114333"},"PeriodicalIF":5.4,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142542495","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Tangerine peel-derived nitrogen-doped carbon dots incorporated chitosan/pullulan-based active packaging film for bread packaging.","authors":"Yoonjung Sul, Ajahar Khan, Jun Tae Kim, Jong-Whan Rhim","doi":"10.1016/j.colsurfb.2024.114339","DOIUrl":"10.1016/j.colsurfb.2024.114339","url":null,"abstract":"<p><p>Citrus peel waste carbon dots based on nitrogen-doped (N-TanCD) were developed by a hydrothermal strategy to deliver active packaging fillers and characterized by transmission electron microscopy, photoluminescence, and Fourier transform infrared analyses. The addition of N-TanCD into chitosan-pululan (CS/Pul@N-TanCD) polymer blend amplified the tensile strength of the composite film by 22.8 %, whereas the antioxidant activities against DPPH and ABTS reached 62.7 % and 91.6 %, respectively. The proposed film showed blocked 98.8 % of UV-A and 100 % of UV-B without affecting the film's transparency. The CS/Pul@N-TanCD film lowered the contamination of L. monocytogenes and E. coli by more than 4 and 5 log CFU/mL, respectively. Sliced bread was packaged using CS/Pul-based films and stored for 12 days at 50 % relative humidity and 25 °C to investigate changes in the quality of the bread. It was found that bread packaged with CS/Pul film integrated with N-TanCD maintained excellent bread quality relating to appearance, moisture content, hardness, weight loss, and total viable bacterial count.</p>","PeriodicalId":279,"journal":{"name":"Colloids and Surfaces B: Biointerfaces","volume":"245 ","pages":"114339"},"PeriodicalIF":5.4,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142556736","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Naringenin loaded fucoidan/polyvinylpyrrolidone nanoparticles protect against folic acid induced acute kidney injury in vitro and in vivo.","authors":"Tao Jiang, Feikai Zhu, Xintao Gao, Xiaochen Wu, Wenyong Zhu, Chuanlong Guo","doi":"10.1016/j.colsurfb.2024.114343","DOIUrl":"10.1016/j.colsurfb.2024.114343","url":null,"abstract":"<p><p>Acute kidney injury (AKI) is a common clinical problem with no effective treatment. Excessive folic acid (FA) induced kidney tubular injury is characterized by oxidative stress and inflammation, and is a common model of AKI. The excellent pharmacological activity of naringenin (NAR) makes it a potential agent for treating AKI, but its poor solubility limits its application. This study prepared NAR loaded nanoparticles (FU/PVP-NAR) using fucoidan (FU) and polyvinylpyrrolidone (PVP) as carriers, with a particle size of 23.96 ± 2.77 nm. In vitro studies showed that FU/PVP-NAR inhibited excessive FA induced proliferation inhibition, accumulation of reactive oxygen species (ROS), and disruption of mitochondrial membrane potential (MMP) of HK-2 cells. Further confirmed that FU/PVP-NAR inhibited FA induced DNA damage and Cyclic GMP-AMP synthase (cGAS)-stimulator of interferon genes (STING) activation. In vivo studies showed that excessive FA induced AKI features in mice, such as elevated serum creatinine (SCr) and blood urea nitrogen (BUN) levels, accompanied by pathological damage to kidney tissues. The above AKI characteristics induced by FA were alleviated by FU/PVP-NAR. FU/PVP-NAR also inhibited the decrease in antioxidant enzyme levels in kidney tissues induced by FA. Furthermore, in vivo mechanism studies indicated that FU/PVP-NAR inhibited the release of inflammatory factors by inhibiting DNA damage-cGAS-STING pathway. In summary, this study provided the possibility for FU/PVP-NAR as a potential candidate drug for treating FA induced AKI.</p>","PeriodicalId":279,"journal":{"name":"Colloids and Surfaces B: Biointerfaces","volume":"245 ","pages":"114343"},"PeriodicalIF":5.4,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142563532","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Fabrication of functional interface on magnetic beads via various amino acids and their application in chemiluminescent immunoassay as carrier.","authors":"Shihong Zhu, Daohang Du, Zhimin Zhao, Xianfeng Chu, Daoxiang Su, Shuli Yu, Ting Tao, Yong Jiang, Zhifei Wang","doi":"10.1016/j.colsurfb.2024.114364","DOIUrl":"10.1016/j.colsurfb.2024.114364","url":null,"abstract":"<p><p>Magnetic polymer microspheres with superparamagnetism, high specificity, and monodispersity play a crucial role in the field of in vitro diagnostics. However, the surface modification process of magnetic beads is often complex, and it remains a significant challenge to prepare high-performance magnetic beads easily. To overcome these drawbacks, herein we fabricated functional interface on magnetic bead with the various amino acid via the ring-opening reaction of amino acids with epoxy groups, with attempt to produce carboxylated magnetic beads (MPS-GA) in a convenient way. Results indicate that when compared to other amino acids, the phenylalanine magnetic beads (MPS-GA1) developed in this study exhibit strong adsorption for mouse immunoglobulin (IgG), streptavidin (SA), and protamine (PA), with an IgG adsorption capacity of 53.5 μg/mg and a coupling capacity of 52.5 μg/mg. It is found that electrostatic forces and hydrophobic interactions are key factors influencing biomolecular interactions. Additionally, these magnetic beads can generate strong chemiluminescent signals, significantly reducing background levels by up to 99.7 %. Therefore, the magnetic beads proposed in this paper can serve as carriers for chemiluminescent immunoassay (CLIA), providing new insights into the synthesis of high-quality magnetic bead.</p>","PeriodicalId":279,"journal":{"name":"Colloids and Surfaces B: Biointerfaces","volume":"245 ","pages":"114364"},"PeriodicalIF":5.4,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142610802","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Enhancing antibacterial performance and stability of implant materials through surface modification with polydopamine/silver nanoparticles.","authors":"Junnan Cui, Haobo Shu, Xin Gu, Shutong Wu, Xiaodan Liu, Pan Cao","doi":"10.1016/j.colsurfb.2024.114327","DOIUrl":"10.1016/j.colsurfb.2024.114327","url":null,"abstract":"<p><p>Implants and various medical devices possess surfaces that are prone to bacterial colonization due to bacterial adhesion and the formation of biofilms. Therefore, inhibiting bacterial colonization is a crucial strategy for preventing infections. Although there have been reports on antibacterial surfaces, the synthetic processes involved are often complex and labor-intensive, which significantly limits their practical applications. Furthermore, there is a lack of studies investigating the interplay between antibacterial performance and stability. In this study, silver ions were reduced to form silver nanoparticles, which were then loaded onto polydopamine (PDA) particles. The successful assembly of PDA-Ag on the surface of the titanium alloy was confirmed through X-ray photoelectron spectroscopy (XPS) and energy-dispersive X-ray spectroscopy (EDS). The morphologies of the micro- and nanoparticles, as well as the surface morphology after deposition, were analyzed using scanning electron microscopy (SEM), transmission electron microscopy (TEM), and a 3D optical profilometer. The abrasion experiments conducted on the three surfaces demonstrated that the TC4@PDA-Ag3 surface exhibited superior friction performance compared to the other two surfaces. Antibacterial and antibacterial stability experiments were conducted on this series of surfaces. The results indicated that the adhesion rate of TC4@PDA-Ag3 on Escherichia coli (E. coli) was 99.68 %, while the antibacterial efficiency against Staphylococcus aureus (S. aureus) was 95.97 %. This study presents a novel approach to address the issue of implant surface infections by demonstrating resistance to bacterial adhesion and colonization, specifically against E. coli and S. aureus.</p>","PeriodicalId":279,"journal":{"name":"Colloids and Surfaces B: Biointerfaces","volume":"245 ","pages":"114327"},"PeriodicalIF":5.4,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142454507","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}