化学•材料最新文献

{"title":"Gelatin methacryloyl @MP196/exos hydrogel induced neutrophil apoptosis and macrophage M2 polarization to inhibit periodontal bone loss.","authors":"Haiwei Zhuo, Shuting Zhang, Hongbo Wang, Jiayin Deng, Xi Zhang","doi":"10.1016/j.colsurfb.2024.114466","DOIUrl":"10.1016/j.colsurfb.2024.114466","url":null,"abstract":"<p><strong>Objectives: </strong>Periodontitis is an inflammatory and destructive disease caused by dental plaque, which can result in the immune microenvironment disorders and loss of periodontal support tissue. In order to promote the restoration of local microenvironment stability, a functional biomaterial Gelatin methacryloyl @MP196/exos based on characteristics of disease occurrence is designed.</p><p><strong>Methods: </strong>Transmission electron microscopy, nanosight particle tracking analysis and western blot analysis were applied to prove the presence of exos in GelMA@MP196/exos. The swelling and degradation rates of GelMA@MP196/exos were evaluated. Cell proliferation, antibacterial ability and cellular uptake and intracellular internalization of exos were assessed in the study. Efferocytosis and M2 polarization of macrophages was estimated and the effects of GelMA@MP196/exos were proved in vivo.</p><p><strong>Results: </strong>GelMA@MP196/exos upregulated the expression of genes and proteins related to neutrophil apoptosis and promoted neutrophil apoptosis, macrophage M2 polarization, and efferocytosis. Furthermore, GelMA@MP196/exos exhibited significant antibacterial activity against Streptococcus gordonii, Fusobacterium nucleatum, and Porphyromonas gingivalis. GelMA@MP196/exos alleviated periodontitis and reduced alveolar bone loss in vivo in rat models.</p><p><strong>Conclusions: </strong>GelMA@MP196/exos can serve as a potential strategy for the treatment of periodontitis.</p><p><strong>Clinical significance: </strong>The main aim of periodontal therapy is to remove dental plaque and eliminate inflammation. However, some patients with low plaque scores and insufficient neutrophil clearance, resulting in poor responsiveness to periodontal therapy. Under the circumstances, local Application of drug that regulate the immune microenvironment had significance in controlling the progression of inflammation.</p>","PeriodicalId":279,"journal":{"name":"Colloids and Surfaces B: Biointerfaces","volume":"248 ","pages":"114466"},"PeriodicalIF":5.4,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142890838","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Recent advances in spatiotemporal control of the CRISPR/Cas9 system.","authors":"Junqi Zhang, Yuzi Zhou, Jie Qiao, Yi Liu","doi":"10.1016/j.colsurfb.2024.114474","DOIUrl":"10.1016/j.colsurfb.2024.114474","url":null,"abstract":"<p><p>The CRISPR/Cas9 gene-editing technology, derived from the adaptive immune mechanisms of bacteria, has demonstrated remarkable advantages in fields such as gene function research and the treatment of genetic diseases due to its simplicity in design, precise targeting, and ease of use. Despite challenges such as off-target effects and cytotoxicity, effective spatiotemporal control strategies have been achieved for the CRISPR/Cas9 system through precise regulation of Cas9 protein activity as well as engineering of guide RNAs (gRNAs). This review provides a comprehensive analysis of the core components and functional mechanisms underlying the CRISPR/Cas9 system, highlights recent advancements in spatiotemporal control strategies, and discusses future directions for development.</p>","PeriodicalId":279,"journal":{"name":"Colloids and Surfaces B: Biointerfaces","volume":"248 ","pages":"114474"},"PeriodicalIF":5.4,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142890856","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Injectable DMM/GelMA hydrogel for diabetic wound healing via regulating mitochondrial metabolism and macrophage repolarization.","authors":"Yuming Shao, Xuan Zhou, Shiqi Zhou, Junhui Long, Linbo Jin, Xiaohua Shi, Linxi Zhou, Yiming Zhang, Dongli Fan","doi":"10.1016/j.colsurfb.2024.114488","DOIUrl":"https://doi.org/10.1016/j.colsurfb.2024.114488","url":null,"abstract":"<p><p>The chronic diabetic wounds represented by diabetes foot ulcers (DFUs) are a worldwide challenge. Excessive production of reactive oxygen species (ROS) and persistent inflammation caused by the impaired phenotype switch of macrophages from M1 to M2 during wound healing are the main culprits of non-healing diabetic wounds. Therefore, an injectable DMM/GelMA hydrogel as a promising wound dressing was designed to regulate the mitochondrial metabolism of macrophages via inhibiting succinate dehydrogenase (SDH) activity and to promote macrophage repolarization towards M2 type. DMM/GelMA hydrogel exhibited good biocompatibility, injectability and water absorption and retention capacity. In vitro studies showed that DMM/GelMA hydrogel inhibited SDH activity, recovered the decrease in mitochondrial membrane potential, and significantly reduced the production of ROS and inflammatory cytokines in the LPS-evoked macrophages. In vivo evaluations and RNA sequencing analysis demonstrated that DMM/GelMA hydrogel downregulated ROS generation, the ratio of M1/M2 macrophages and pro-inflammatory cytokine production in the full-thickness skin wound model in the diabetic mice. Additionally, DMM/GelMA hydrogel improved the wound-healing quality with thicker epidermis, more collagen deposition and higher ratio of collagen I/III by sustained release of DMM. These findings indicate this hydrogel has a great potential to be a biocompatible, injectable and anti-inflammatory dressing for better diabetic wound healing.</p>","PeriodicalId":279,"journal":{"name":"Colloids and Surfaces B: Biointerfaces","volume":"248 ","pages":"114488"},"PeriodicalIF":5.4,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143027484","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Corrigendum to \"Sodium alginate hydrogel containing platelet-rich plasma for wound healing\" [Colloids Surf. B Biointerfaces 222 (2023) 113096].","authors":"Ting Wang, Wanwan Yi, Yu Zhang, Hang Wu, Hengwei Fan, Jiulong Zhao, Shige Wang","doi":"10.1016/j.colsurfb.2024.114462","DOIUrl":"10.1016/j.colsurfb.2024.114462","url":null,"abstract":"","PeriodicalId":279,"journal":{"name":"Colloids and Surfaces B: Biointerfaces","volume":"248 ","pages":"114462"},"PeriodicalIF":5.4,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142862587","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Corrigendum to \"Effects of gold nanostructures on differentiation of mesenchymal stem cells\" [Colloid. Surf. B: Biointerfaces 184 (2019) 110494].","authors":"Long Yuan, Xiaowei Qi, Gaoping Qin, Qi Liu, Fan Zhang, Yong Song, Jun Deng","doi":"10.1016/j.colsurfb.2024.114469","DOIUrl":"10.1016/j.colsurfb.2024.114469","url":null,"abstract":"","PeriodicalId":279,"journal":{"name":"Colloids and Surfaces B: Biointerfaces","volume":"248 ","pages":"114469"},"PeriodicalIF":5.4,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142875574","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Self-augmented catabolism mediated by Se/Fe co-doped bioceramics boosts ROS storm for highly efficient antitumor therapy of bone scaffolds.","authors":"Wenjing Yang, Chenhang Ding, Yibing Ji, Chongxian He, Feilong Xiong, Wurikaixi Aiyiti, Cijun Shuai","doi":"10.1016/j.colsurfb.2024.114477","DOIUrl":"10.1016/j.colsurfb.2024.114477","url":null,"abstract":"<p><p>The overexpression of glutathione (GSH) within the tumor microenvironment has long been considered as the major obstacle for reactive oxygen species (ROS)-based antitumor therapies. To address this challenge, a selenite (SeO₃^2 -) and ferric ion co-doped hydroxyapatite (SF-HAP) nanohybrid was synthesized, which is then introduced into poly-L-lactic acid (PLLA) to prepare porous scaffold by selective laser sintering to continuously release Fe³⁺ and SeO₃^2- ions. Of great significance is the released SeO₃^2- catabolize GSH to generate superoxide anion (O₂^•-) rather than directly eliminating GSH, thereby reversing the obstacle posed by its overexpression and achieving a \"waste-to-treasure\" transformation. The newly generated O₂^•- synergizes with the hydroxyl radicals (•OH) produced by the Fenton reaction between Fe^3+/2+ and hydrogen peroxide, triggering high concentration ROS storms. Collectively, the PLLA/SF-HAP scaffold mediated self-augmented catabolism dynamic progress significantly raised intracellular ROS levels, almost twice as much as PLLA/Fe-HAP scaffold, thereby effectively inducing tumor cell apoptosis. The study provides an innovative inspiration for ROS-based antitumor therapies.</p>","PeriodicalId":279,"journal":{"name":"Colloids and Surfaces B: Biointerfaces","volume":"248 ","pages":"114477"},"PeriodicalIF":5.4,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142908905","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Detection of the SARS-CoV-2 nucleoprotein by electrochemical biosensor based on molecularly imprinted polypyrrole formed on self-assembled monolayer.","authors":"Viktorija Liustrovaite, Vilma Ratautaite, Almira Ramanaviciene, Arunas Ramanavicius","doi":"10.1016/j.bios.2024.117092","DOIUrl":"10.1016/j.bios.2024.117092","url":null,"abstract":"<p><p>Herein, we report the development and characterisation of an electrochemical biosensor with a polypyrrole (Ppy)-based molecularly imprinted polymer (MIP) for the serological detection of the recombinant nucleocapsid protein of SARS-CoV-2 (rN). The electrochemical biosensor utilises a Ppy-based MIP formed on a self-assembled monolayer (SAM) at the gold interface to enhance Ppy layer stability on the screen-printed electrode (SPE). Electrochemical impedance spectroscopy (EIS) and square wave voltammetry (SWV) were employed for the electrochemical characterisation of screen-printed gold electrodes (SPGEs) modified with MIP or non-imprinted polymer (NIP) layers. Removing the rN protein template from the MIP layer increased electron transfer and decreased impedance, indicating the specificity of molecular imprinting. The electrochemical biosensor with a Ppy-based MIP exhibited higher sensitivity than the NIP counterpart, demonstrating its potential for selective rN protein detection. The limit of detection 0.4 nM and 0.2 nM and the limit of quantification 1.3 nM and 0.66 nM values obtained through SWV and EIS, respectively, highlight the biosensor's ability to detect low target protein concentrations. The specificity test confirmed minimal nonspecific binding, reinforcing the reliability of the novel electrochemical sensor with a Ppy-based MIP.</p>","PeriodicalId":259,"journal":{"name":"Biosensors and Bioelectronics","volume":"272 ","pages":"117092"},"PeriodicalIF":10.7,"publicationDate":"2025-03-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142941972","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Mid-infrared enhanced spectrochemical detection using azide vibrational probes.","authors":"Valentina Di Meo, Gennaro Sanità, Angela Oliver, Annamaria Sandomenico, Massimo Moccia, Ivo Rendina, Alessio Crescitelli, Vincenzo Galdi, Menotti Ruvo, Emanuela Esposito","doi":"10.1016/j.bios.2024.117083","DOIUrl":"https://doi.org/10.1016/j.bios.2024.117083","url":null,"abstract":"<p><p>Spectrochemical analysis of trace elements in complex matrices is crucial across various fields of science, industry, and technology. However, this analysis is often hindered by background interference and the challenge of detecting ultralow analyte concentrations. Surface Enhanced Infrared Absorption (SEIRA) spectroscopy is emerging as a viable technique to address these challenges as it can successfully reveal soluble and unmodified analytes in a label-free manner through their interactions with a bioreceptor following site-specific labeling with small infrared-active probes. In this study, we present and demonstrate an advanced method for mid-infrared spectroscopy utilizing a pixeled SEIRA substrate coupled with a peculiar infrared-active vibrational probe. We select a small azide moiety as the vibrational tag since its signature around 2100 cm^-1 is in the cell- and protein-silent window and its small size preserves the structure and biological function of the protein it integrates into. As model bioreceptor, we utilize an antigen-binding fragment (Fab') derived from the therapeutic antibody trastuzumab, modified with azidoacetic acid, and its Her2 antigen as the soluble analyte. Employing mid-infrared SEIRA spectroscopy, we are able to monitor the immobilization of the azide-modified Fab', and demonstrate the detection of analyte quantities as low as 83 amol within an area of 100 μm².</p>","PeriodicalId":259,"journal":{"name":"Biosensors and Bioelectronics","volume":"272 ","pages":"117083"},"PeriodicalIF":10.7,"publicationDate":"2025-03-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142997328","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Lateral flow analysis test strips based on aggregation-induced emission technique: Principle, design, and application.","authors":"Shan Liu, Yongqi Li, Yunchao Yang, Xue Li, Lijun Wang, Xun Xiao, Chen-Zhong Li","doi":"10.1016/j.bios.2024.117058","DOIUrl":"10.1016/j.bios.2024.117058","url":null,"abstract":"<p><p>This review examines the potential of aggregation-induced luminescence (AIE) materials in lateral flow assays (LFA) to enhance the sensitivity and specificity of a range of assay applications. LFA is a straightforward and effective paper-based platform for the rapid detection of target analytes in mixtures. Its simple design, low cost, and ease of operation are among the most attractive advantages of LFA. The signal reporting label, which constitutes the core component of LFA detection, is of paramount importance for enhancing the sensitivity of the detection process. The sensitivity of traditional LFA signal labels is insufficient for the detection of biomarkers at low concentrations. To address this issue, AIE materials have been developed in recent years. These materials can significantly enhance the luminescence intensity at high concentrations or in aggregated states, exhibiting excellent photostability and a high signal-to-noise ratio. They possess the advantages of high quantum yields, good photostability, and strong fluorescence, rendering them suitable for a variety of applications, including medical diagnostics, food safety, and environmental monitoring. This review therefore provides an overview of the operational principles of AIE and LFA, details the selection of AIE materials, the design of the platform and their applications, and reviews the latest research. Notable examples include the detection of viral pathogens, bacterial and mycotoxin contamination, antibiotic residues, and pesticide residues. The integration of AIE materials in LFA technology has demonstrated the potential for rapid, accurate, and cost-effective diagnostics. However, challenges remain in large-scale synthesis and multiplexed assays. The paper concludes with a discussion of current limitations and future directions for the technology.</p>","PeriodicalId":259,"journal":{"name":"Biosensors and Bioelectronics","volume":"272 ","pages":"117058"},"PeriodicalIF":10.7,"publicationDate":"2025-03-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142918664","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Construction of in situ modulated controlled growth of MOF-on-mof impedimetric assembly for the practical minimal level assessment of anti-mullerian hormone.","authors":"Wan-Zheng Fang, Sathyadevi Palanisamy, Priya Vijayaraghavan, Chiao-Yun Chen, Shun-Long Weng, Chung-Shi Yang, Yun-Ming Wang","doi":"10.1016/j.bios.2024.117113","DOIUrl":"10.1016/j.bios.2024.117113","url":null,"abstract":"<p><p>Anti-mullerian hormone (AMH) detection receives much attention since it is used as an ideal biomarker for quantitative assessment of ovarian reserve. The present study proposed a first report on the use of MOF-on-MOF as an electrochemical sensor for recognizing AMH in buffer and serum media. The MOF-on-MOF, MIL-88 B@UiO66NH₂ was synthesized by the internal extended growth method (IEGM) involving MIL-88 B on UiO66NH₂ by in situ method for the first time. MOF matrix could be established to form a three-dimensional (3D) core-shell hybrid unit using MOFs with distinct characteristics. The morphology, structural characteristics, and electrochemical performance of MIL-88 B@UiO66NH₂ were studied. It was successfully used for AMH sensing to demonstrate the detection performance of the internal extended growth method (IEGM) grown MIL-88 B@UiO66NH₂ made immunosensor. The electrochemical results indicated that MOF-on-MOF exhibited linear EIS response for AMH concentration varying from 100 ng/mL to 1 fg/mL. Further, the immunosensor displayed high specificity and sensitivity for AMH detection. The fabricated sensor attained a remarkable limit of detection (LOD) of 1.07 fg/mL and 0.82 fg/mL, when studied in PBS and 10% serum buffer media, respectively. The biosensor achieved the limit of quantification (LOQ) of 3.25 fg/mL and 2.5 fg/mL, respectively, when analyzed in PBS buffer and 10% serum buffer. The significant results emphasized that the fabricated biosensor holds a promising potential to act as an appropriate tool for rapid assessment of AMH levels.</p>","PeriodicalId":259,"journal":{"name":"Biosensors and Bioelectronics","volume":"272 ","pages":"117113"},"PeriodicalIF":10.7,"publicationDate":"2025-03-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142925889","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}