ACS Applied Bio Materials最新文献

{"title":"Antifouling and Bactericidal Zwitterionic Polymer Coatings with Synergistic Inhibitory and Killing Properties.","authors":"Fei Ma, Xinde Tang, Yanan Chao, Xinyan Wang, Xuanjie Zheng, Ruixin Fu, Zihao Tang, Laixue Pang, Fuying Dong, Haixia Cheng, Peng Wang","doi":"10.1021/acsabm.4c01776","DOIUrl":"10.1021/acsabm.4c01776","url":null,"abstract":"<p><p>Polymeric coatings that combine resistance to adhesion (\"defending\") and killing (\"attacking\") of biocontaminants were proposed to endow the surface with nonadhesive and bactericidal capabilities. In contrast, a zwitterionic copolymer P(GMA-<i>co</i>-DMAPS) with antifouling groups ([2-(methacryloyloxy)ethyl]dimethyl-(3-sulfopropyl)ammonium hydroxide, DMAPS) and a zwitterionic/cationic copolymer P(GMA-<i>co</i>-DMAPS-<i>co</i>-DMC) with bactericidal groups ([2-(methacryloyloxy)ethyl]trimethylammonium chloride, DMC) were synthesized, of which the latter exhibited synergistic inhibitory and killing properties. The distinct feed ratios of monomers were conducted, and the optimal molar ratio was obtained. The polymer coating was chemically bonded to the surface of substrates such as stainless steel via the addition-elimination reaction of epoxy groups and hydroxyl groups. Stainless steel surfaces grafted with G20D80-5DMC exhibited fine antifouling and bactericidal properties. The results showed that a highly efficient defending-attacking synergistic effect was achieved with antiprotein adsorption of more than 90% and bactericidal rates against <i>Staphylococcus aureus</i> and <i>Escherichia coli</i> of 98.6% and 96.6%, respectively. The polymers proposed in this study can effectively play an antifouling and antibacterial synergistic role and can be grafted onto substrates through a simple and effective method, showing attractive potential in marine, biomedical, and industrial applications.</p>","PeriodicalId":2,"journal":{"name":"ACS Applied Bio Materials","volume":" ","pages":"1635-1645"},"PeriodicalIF":4.6,"publicationDate":"2025-02-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143021264","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Deciphering Binding Potential of Naphthalimide-Coumarin Conjugate with c-MYC G-Quadruplex for Developing Anticancer Agents: A Spectroscopic and Molecular Modeling Approach.","authors":"Saurabh Gupta, Vijay Luxami, Kamaldeep Paul","doi":"10.1021/acsabm.4c01388","DOIUrl":"10.1021/acsabm.4c01388","url":null,"abstract":"<p><p>It has been well accumulated that G-quadruplex (G4-DNA) has great anticancer relevance, and various heterocyclic moieties have been synthesized and examined as potent G4-DNA binders with promising anticancer activity. Here, we have synthesized a series of naphthalimide-triazole-coumarin conjugates by substituting various amines and further examine their anticancer activity against 60 human cancer cell lines at 10 μM. One and five dose concentration results reveal low values of MG-MID GI₅₀ for compounds including <b>8a</b> (3.18 μM), <b>8b</b> (13.11 μM), <b>8e</b> (7.68 μM) and <b>8f</b> (1.75 μM). Further cell apoptosis manifests that all compounds can induce cell apoptosis in cancer cells by stabilizing the c-MYC promoter G-quadruplex. Therefore, the G-quadruplex-mediated pathway may be responsible for the apoptosis that these naphthalimide-coumarin compounds caused in cancer cells. Therefore, multispectroscopic techniques are employed to evaluate the binding of molecules with c-MYC G4-DNA where all four molecules readily bind to G4-DNA and stabilize it with a high binding constant, leading to inhibition of cancer cells and apoptosis of cancer cells. Binding studies toward ct-DNA disclose that these compounds do not interact with ds-DNA and thus selectively target G4-DNA to exert their anticancer activity. All the active compounds have greater affinity toward Human Serum Albumin (HSA) and can readily bind with HSA with a binding constant of 12 × 10⁴ M^-1 (<b>8a</b>), 13.0 × 10⁴ M^-1 (<b>8b</b>), 14.2 × 10⁴ M^-1 (<b>8e</b>), 16.3 × 10⁴ M^-1 (<b>8f</b>). Thus, the results disclose that inhibition and killing of cancer cells by these derivatives feasibly occur due to their ability to interact with c-MYC G-quadruplex forming promoters and their high affinity toward HSA unfold potent anticancer agents and can be taken further for clinical trials.</p>","PeriodicalId":2,"journal":{"name":"ACS Applied Bio Materials","volume":" ","pages":"1077-1096"},"PeriodicalIF":4.6,"publicationDate":"2025-02-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143050949","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Anti-Infective Bacteriophage Immobilized Nitric Oxide-Releasing Surface for Prevention of Thrombosis and Device-Associated Infections.","authors":"Vijay Singh Gondil, Morgan Ashcraft, Sama Ghalei, Anil Kumar, Sarah N Wilson, Ryan Devine, Hitesh Handa, Elizabeth J Brisbois","doi":"10.1021/acsabm.4c01638","DOIUrl":"10.1021/acsabm.4c01638","url":null,"abstract":"<p><p>The treatment of critically ill patients has made great strides in the past few decades due to the rapid development of indwelling medical devices. Despite immense advancements in the design of these devices, indwelling medical device-associated infections and thrombosis are two major clinical problems that may lead to device failure and compromise clinical outcomes. Antibiotics are the current treatment choice for these infections; however, the global emergence of antibiotic-resistance and their biofilm formation abilities complicate the management of such infections. Moreover, systemic administration of anticoagulants has been used to counter medical device-induced thrombosis, but a range of serious adverse effects associated with all types of available anticoagulants entails exploring alternative options to counter device-associated thrombosis. In this study, bacteriophages (phages) were covalently immobilized on polydimethylsiloxane (PDMS) surface containing the nitric oxide (NO) donor <i>S</i>-nitroso-<i>N</i>-acetylpenicillamine (SNAP) via SNAP impregnation method. This dual strategy combines the targeted antibacterial activity of phages against bacterial pathogens with the antibacterial-antithrombotic activity of NO released from the polymeric surface. The PDMS, SNAP-PDMS, phage-immobilized PDMS (PDMS-Phage), and phage-immobilized SNAP-PDMS (SNAP-PDMS-Phage) surfaces were characterized for their surface topology, elemental composition, contact angle, SNAP loading, NO release and phage distribution. SNAP-PDMS and SNAP-PDMS-Phage surfaces showed similar and consistent NO release profiles over 24 h of incubation. Immobilization of whole phages on PDMS and SNAP-PDMS was achieved with densities of 2.4 ± 0.54 and 2.1 ± 0.33 phages μm^-2, respectively. Immobilized phages were found to retain their activity, and SNAP-PDMS-Phage surfaces showed a significant reduction in planktonic (99.99 ± 0.08%) as well as adhered (99.80 ± 0.05%) <i>Escherichia coli</i> as compared to controls in log killing assays. The SNAP-PDMS-Phage surfaces also exhibited significantly reduced platelet adhesion by 64.65 ± 2.95% as compared to control PDMS surfaces. All fabricated surfaces were found to be nonhemolytic and do not exhibit any significant cytotoxic effects toward mammalian fibroblast cells. This study is the first of its kind to demonstrate the combinatorial pertinence of phages and NO to prevent antibiotic-resistant/sensitive bacterial infections and thrombosis associated with indwelling medical devices.</p>","PeriodicalId":2,"journal":{"name":"ACS Applied Bio Materials","volume":" ","pages":"1362-1376"},"PeriodicalIF":4.6,"publicationDate":"2025-02-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143077858","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The Compression-Dominated Ultrasound Response of Poly(<i>n</i>-butyl cyanoacrylate) Hard-Shelled Microbubbles Induces Significant Sonoporation and Sonopermeation Effects <i>In Vitro</i>.","authors":"Julia Blöck, Hongchen Li, Gonzalo Collado-Lara, Klazina Kooiman, Anne Rix, Junlin Chen, Christopher Hark, Harald Radermacher, Céline Porte, Fabian Kiessling","doi":"10.1021/acsabm.4c01551","DOIUrl":"10.1021/acsabm.4c01551","url":null,"abstract":"<p><p>The process of locally increasing the permeability of cell membranes or cell layers is referred to as sonoporation or sonopermeation, respectively, and opens up perspectives for drug delivery in cancer treatment by facilitating enhanced local drug accumulation. These effects are mediated by ultrasound-activated microbubbles in close proximity to cells. Here, the selection of ultrasound settings according to the intended effect on the biological tissue remains a challenge, especially for broadly size-distributed microbubbles, which show a heterogeneous response to ultrasound. For this purpose, we have analyzed the general response of narrower size-distributed poly(<i>n</i>-butyl cyanoacrylate) hard-shelled microbubbles to ultrasound via ultra-high-speed imaging and evaluated their ability to stimulate sonoporation and sonopermeation <i>in vitro</i> compared to lipid soft-shelled microbubbles. Ultra-high-speed imaging of hard-shelled microbubbles revealed either a compression-dominated or compression-only response at peak negative acoustic pressures higher than 165 kPa and an onset of bursting at 500 kPa. The <i>in vitro</i> experiments demonstrated that the hard-shelled microbubbles induced significant sonoporation and sonopermeation effects, also when only compressing at 300 kPa peak neagtive pressure. Compared to soft-shelled microbubbles, the effects were less prominent, which was attributed to differences in their ultrasound responses and size distributions. This <i>in vitro</i> validation of hard-shelled microbubbles qualifies them for future <i>in vivo</i> applications, which would benefit from their narrow size distribution, thereby allowing more control of their therapeutic effect by suitably adjusting the ultrasound parameters.</p>","PeriodicalId":2,"journal":{"name":"ACS Applied Bio Materials","volume":" ","pages":"1240-1250"},"PeriodicalIF":4.6,"publicationDate":"2025-02-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143121652","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"In Vitro Assessment of Gallium Nanoalloy Hydrogels for Antimicrobial and Wound Healing Applications.","authors":"Richard Bright, Soroopan Sivanantha, Andrew Hayles, Tan Phuoc Ton, Neethu Ninan, Xuan Luo, Krasimir Vasilev, Vi Khanh Truong","doi":"10.1021/acsabm.4c01182","DOIUrl":"10.1021/acsabm.4c01182","url":null,"abstract":"<p><p>Chronic and recurring wounds pose a significant challenge in modern healthcare, leading to substantial morbidity. These wounds allow pathogens to colonize, potentially resulting in local and systemic infections. Current interventions need to be revised and become increasingly less reliable due to the emergence of antibiotic resistance. In the present study, we aim to address these issues by fabricating hydrogels impregnated with gallium-based nanoalloys for their antimicrobial activity. Gallium liquid metal nanoparticles (approximately 100 nm in diameter) were alloyed in different combinations with bismuth and silver ions through a galvanic replacement reaction. These multimetallic hydrogels showed favorable antibacterial activity against the Gram-positive <i>Staphylococcus aureus</i> and the Gram-negative <i>Pseudomonas aeruginosa</i>, as observed with fluorescence microscopy and inhibition assays. The multimetallic hydrogels showed no toxicity against murine macrophages or human dermal fibroblasts and enhanced in vitro wound healing. The development of these innovative gallium-based hydrogels represents a promising strategy to combat chronic wounds and their associated complications, offering an effective alternative to current antimicrobial treatments amidst rising antibiotic resistance.</p>","PeriodicalId":2,"journal":{"name":"ACS Applied Bio Materials","volume":" ","pages":"1017-1026"},"PeriodicalIF":4.6,"publicationDate":"2025-02-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142453151","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Multiscale Hierarchical Micro- and Nano-Surface Induced by High-Repetition-Rate Femtosecond Laser Promote Peri-Implant Osseointegration.","authors":"Weiwei Guo, Xu He, Jianye Song, Zhonghua Cao, Wenhui Hu, Yinghui Tan, Shiwu Dong, Yuncan Ma, Kun Wang","doi":"10.1021/acsabm.4c01759","DOIUrl":"10.1021/acsabm.4c01759","url":null,"abstract":"<p><p>Micro- and nanomorphological modification and roughening of titanium implant surfaces can enhance osseointegration; however, the optimal morphology remains unclear. Laser processing of implant surfaces has demonstrated significant potential due to its precision, controllability, and environmental friendliness. Femtosecond lasers, through precise optimization of processing parameters, can modify the surface of any solid material to generate micro- and nanomorphologies of varying scales and roughness. Inspired by the multiscale micro- and nanostructures of natural bone tissue, this study employed a high-repetition-rate femtosecond laser to fabricate three distinct micro- and nanomorphologies on titanium implant surfaces, characterized by low (LTi), medium (MTi), and high (HTi) roughness, exhibiting multiscale coexistence. Comprehensive characterization of the modified surfaces included analysis of morphology, roughness, wettability, and elemental composition. Furthermore, in vitro and in vivo experiments were conducted to evaluate osteogenic differentiation and osseointegration capabilities. Results revealed that the HTi surface, exhibiting high roughness, presents a multiscale hierarchical micro- and nanostructure composed of micrometer-sized spheres, submicrometer-sized corrugations, and nanometer-sized particles. In vitro studies demonstrated that the HTi surface promoted earlier adhesion, spreading, and enhanced osteogenic differentiation of osteoblasts, while in vivo studies indicated improved bone formation and osseointegration. In conclusion, multiscale hierarchical micro- and nanosurfaces with high roughness generated by high-repetition-rate femtosecond laser processing hold considerable promise for titanium implant applications.</p>","PeriodicalId":2,"journal":{"name":"ACS Applied Bio Materials","volume":" ","pages":"1621-1634"},"PeriodicalIF":4.6,"publicationDate":"2025-02-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142995998","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Antimicrobial and Antibiofilm Activities of Urinary Catheter Incorporated with ZnO-Carbon Nanotube.","authors":"Seong Hwan Kim, Dong Yun Kim, Je Seon Park, Myungchan Park, Min Chul Park","doi":"10.1021/acsabm.4c01658","DOIUrl":"10.1021/acsabm.4c01658","url":null,"abstract":"<p><p>Urinary tract infections are among the most common nosocomial infections, with the majority being catheter-associated urinary tract infections (CAUTIs). This study demonstrated that an antimicrobial and antibiofilm urinary catheter containing zinc oxide-carbon nanotubes (ZnO-CNT) can inhibit CAUTIs in patients. ZnO-CNT polymers were synthesized by mixing ZnO and CNT using a high-shear mixer, and the synthesized ZnO-CNT polymers were incorporated into a silicone matrix to produce a ZnO-CNT urinary catheter. Scanning electron microscopy was used to evaluate the morphology and atomic composition of the polymer and urinary catheter, which contained 1.23% Zn element. Dynamic light scattering analysis showed an average particle size of 253.4 nm with a zeta potential of +21.4 mV. To assess antimicrobial activity, the ZnO-CNT polymer and urinary catheter were tested using minimum inhibitory concentration (MIC) and antibiofilm assays. The ZnO-CNT polymers exhibited MIC values of 0.0078, 1, 0.00625, and 0.0039% against <i>E. coli</i>, <i>P. aeruginosa</i>, <i>E. faecalis</i>, and <i>S. aureus</i>, respectively. Antibiofilm assays conducted at concentrations ranging from 1/4 to 2 × MIC demonstrated effective inhibition of biofilm formation at 1 × MIC or lower concentrations in <i>E. coli</i> and <i>P. aeruginosa</i>. The ZnO-CNT urinary catheter inhibited biofilm formation by 53.42 and 56.44% after 120 h of incubation compared to the silicone urinary catheter against <i>E. coli</i> and <i>P. aeruginosa</i>, respectively. These findings suggest that the ZnO-CNT urinary catheter could not only replace commonly used silicone catheters to reduce patient discomfort but also serve as a viable alternative to antimicrobial urinary catheters coated with metal alloys such as silver, gold, or palladium.</p>","PeriodicalId":2,"journal":{"name":"ACS Applied Bio Materials","volume":" ","pages":"1397-1405"},"PeriodicalIF":4.6,"publicationDate":"2025-02-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143057478","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Electric Field Polarity Controls Distribution of Viral Bioreceptors within Near-Field Electrospun Biohybrid Microfiber Optical Biosensors.","authors":"Stephen T Hsieh, Jordyn M Watkins, Zaira Alibay, Joshua M Plank, Kalie Inouye, Nosang V Myung, Elaine D Haberer","doi":"10.1021/acsabm.4c01761","DOIUrl":"https://doi.org/10.1021/acsabm.4c01761","url":null,"abstract":"<p><p>Microorganisms (e.g., bacteria, fungi, and viruses) add indispensable functionality to a range of electrospun polymer materials and devices. The optimal distribution of bioactive agents on either the interior or exterior of the fiber is application-specific. Current microbe surface immobilization strategies and core-confinement techniques continue to pose a number of challenges. Here, we explore a simple strategy, utilizing electrostatic forces, to control the migration and surface concentration of the M13 bacteriophage within near-field electrospun poly(vinyl alcohol) (PVA) microfibers. Both the surface charge of the electrospun virus and the applied electric field polarity altered microbe placement. When doped with Rhodamine 6G (R6G), the circular microfiber cross sections formed active whispering gallery mode (WGM) resonators. These relatively high-quality (<i>Q</i>) optical cavities enabled us to sensitively probe the virus content of their outer layer, while functioning as label-free optical biosensors with phage-based streptavidin biorecognition elements. Coulomb forces displayed significant control over M13 surface coverage during near-field electrospinning, increasing biosensor response by nearly a factor of 4 to 1310 nM streptavidin. These findings are an important demonstration of electrostatic forces as a simple, yet adaptable method to enhance biohybrid fiber functionality and performance by tailoring microbe distribution.</p>","PeriodicalId":2,"journal":{"name":"ACS Applied Bio Materials","volume":" ","pages":""},"PeriodicalIF":4.6,"publicationDate":"2025-02-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143424449","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Preparation of Metal-Polyphenol Modified Zeolitic Imidazolate Framework-8 Nanoparticles for Cancer Drug Delivery.","authors":"Giao Thuy-Quynh Vu, Luan Minh Nguyen, Kim Ngan Nguyen Do, Dieu Linh Tran, Toi Van Vo, Dai Hai Nguyen, Long Binh Vong","doi":"10.1021/acsabm.4c01608","DOIUrl":"10.1021/acsabm.4c01608","url":null,"abstract":"<p><p>With the rising incidence of cancer, chemotherapy has become a widely used treatment approach. However, the use of anticancer drugs such as doxorubicin (DOX) poses significant long-term risks due to its nonspecific distribution and severe side effects. Therefore, developing a nanoparticle-based drug delivery system (DDS) that enhances the bioavailability of DOX specifically to cancer cells is crucial while minimizing its side effects on normal cells. This study employed zeolitic imidazolate framework-8 (ZIF-8) as a DDS to encapsulate DOX using a one-pot method. The surface of this system was subsequently modified with a copper-gallic acid (Cu-GA) complex to form the Cu-GA/DOX@ZIF-8 (CGDZ) system. The CGDZ system effectively encapsulates DOX and demonstrates pH-responsive drug release, facilitating controlled drug release in the acidic environment of cancer cells. Furthermore, the Cu-GA coating enhances the biocompatibility of the material, reduces drug toxicity in normal endothelial cells (BAECs) due to the antioxidant feature of modified GA, and maintains the efficacy and intracellular trafficking of DOX in colon cancer cells (C-26). Interestingly, CGDZ nanoparticles showed significantly higher toxicity against cancer cells as compared to unmodified systems and free DOX. Overall, CGDZ exhibited significant <i>in vitro</i> efficacy in targeting cancer cell lines while reducing the toxicity of DOX, offering a novel and effective nanoparticle system for targeted cancer treatment.</p>","PeriodicalId":2,"journal":{"name":"ACS Applied Bio Materials","volume":" ","pages":""},"PeriodicalIF":4.6,"publicationDate":"2025-02-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143412249","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Multilayered Freestanding Porous Polycarbonate Nanosheets with Directed Protein Permeability for Cell-Encapsulated Devices.","authors":"Nanami Zushi, Megumi Takuma, Atena Endo, Mahiro Suzuki, Yumeng Wu, Nobuaki Shiraki, Shoen Kume, Toshinori Fujie","doi":"10.1021/acsabm.4c01446","DOIUrl":"https://doi.org/10.1021/acsabm.4c01446","url":null,"abstract":"<p><p>Implantable pancreatic β cell-encapsulated devices are required for the treatment of type 1 diabetes. Such devices should enable a semipermeable membrane to release insulin in response to glucose levels while avoiding immune reactions. Micrometer-thick track-etched porous polycarbonate (PC) membranes have been used for this purpose. However, the immediate release of insulin remains a challenge in the development of such semipermeable membranes. Herein, we attempted to develop a freestanding polymeric ultrathin film (nanosheet) with a porous structure that can be used in a cell-encapsulated device. Specifically, we fabricated a nonbiodegradable, porous PC nanosheet to enhance molecular permeability. The nanosheet was multistacked to ensure the controlled permeability of proteins of various molecular weights, such as insulin and IgG. The porous PC nanosheet was prepared by gravure coating using a blend solution comprising PC and polystyrene (PS) to induce macro-phase separation of the PC and PS. When the PC:PS weight ratio of the mixture was reduced to 3:1, we succeeded in fabricating a porous PC nanosheet (thickness: 100 nm, diameter: < 2.5 μm). A triple layer of such porous nanosheets with various pore sizes demonstrated 10 times less protein clogging, 10 times higher insulin permeability, and comparable IgG-blocking capability compared with commercially available porous PC membranes (thickness: 10 μm). Finally, we demonstrated that a cell-encapsulated device equipped with the multilayered porous PC nanosheet as a permeable membrane preserved the glucose response level of insulin-producing cells before, during, and after the cell-encapsulation process. We believe that cell-encapsulated devices equipped with such porous PC nanosheets will enable immediate insulin release in response to changes in glucose levels.</p>","PeriodicalId":2,"journal":{"name":"ACS Applied Bio Materials","volume":" ","pages":""},"PeriodicalIF":4.6,"publicationDate":"2025-02-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143412244","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}