ACS Applied Bio Materials最新文献

{"title":"Preparation and Antibacterial Activity Evaluation of Daphnetin-Loaded Poloxamers/Polyvinylpyrrolidone Thermosensitive Hydrogels.","authors":"Junhong Fan, Fengli An, Shaohua Li, Yuqin Guo, Haolan Zhang, Yaxin Zhang, Yujie Cao, Lan Yu","doi":"10.1021/acsabm.4c01348","DOIUrl":"https://doi.org/10.1021/acsabm.4c01348","url":null,"abstract":"<p><p>Antibiotic misuse and bacterial resistance are pressing issues threatening public health. Natural plant extracts with bactericidal properties offer potential alternatives to reduce or replace antibiotic use. This study aims to develop a thermosensitive hydrogel containing daphnetin (DAP-TG) using poloxamers 407 (P407), polyvinylpyrrolidone (PVP), and poloxamers 188 (P188). We systematically evaluated the gel's antibacterial activity against <i><i>Escherichia coli</i></i> (<i>E. coli</i>) and <i>Staphylococcus aureus</i> (<i>S. aureus</i>), as well as its antibacterial mechanisms. By examining the gelation temperature and time, degradation time, and in vitro release performance of DAP-TG, we produced a sustained-release DAP-TG with a rapid phase transition at (31.6 ± 0.1) °C. Its structure was characterized through Fourier transform infrared (FTIR) spectroscopy and scanning electron microscopy (SEM). The results indicated that the DAP thermosensitive hydrogel was formed and presented a 3D network spatial structure. The biocompatibility of DAP-TG was explored through the hemolysis test and cytotoxicity test. The results indicated that DAP-TG possessed excellent biocompatibility. The antibacterial efficacy of DAP-TG against <i>E. coli</i> and <i>S. aureus</i> was assessed using minimum inhibitory concentration (MIC), minimum bactericidal concentration (MBC), growth curve, and inhibition zone tests. Results showed that DAP-TG exhibited excellent antibacterial activity against both <i>E. coli</i> and <i>S. aureus</i>, with MIC values of 1.28 and 0.32 mg/mL. The antibacterial mechanism of DAP-TG was preliminarily explored through the investigation of bacterial cell content leakage, AKP leakage, membrane permeability, SEM, ROS production, and biofilm inhibition activity. DAP-TG induced irreversible damage to the cell membranes of <i>E. coli</i> and <i>S. aureus</i>, resulting in enhanced permeability, elevated ROS levels, and inhibited biofilm formation. Our study indicates that DAP-TG exhibits effective sustained-release and antibacterial properties against <i>E. coli</i> and <i>S. aureus</i> in vitro, making it a promising candidate for antibacterial applications in food and pharmaceutical products.</p>","PeriodicalId":2,"journal":{"name":"ACS Applied Bio Materials","volume":" ","pages":""},"PeriodicalIF":4.6,"publicationDate":"2024-12-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142811400","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Recent Plasmonic Gold- and Silver-Assisted Raman Spectra for Advanced SARS-CoV-2 Detection.","authors":"Nguyễn Hoàng Ly, Jaebum Choo, Lalitha Gnanasekaran, Tejraj Malleshappa Aminabhavi, Yasser Vasseghian, Sang-Woo Joo","doi":"10.1021/acsabm.4c01457","DOIUrl":"https://doi.org/10.1021/acsabm.4c01457","url":null,"abstract":"<p><p>COVID-19 has become one of the deadliest epidemics in the past years. In efforts to combat the deadly disease besides vaccines, drug therapies, and facemasks, significant focus has been on designing specific methods for the sensitive and accurate detection of SARS-CoV-2. Of these, surface-enhanced Raman scattering (SERS) is an attractive analytical tool for the identification of SARS-CoV-2. SERS is the phenomenon of enhancement of Raman intensity signals from molecular analytes anchored onto the surfaces of roughened plasmonic nanomaterials. This work gives an updated summary of plasmonic gold nanomaterials (AuNMs) and silver nanomaterials (AgNMs)-based SERS technologies to identify SARS-CoV-2. Due to extreme \"hot spots\" promoting higher electromagnetic fields on their surfaces, different shapes of AuNMs and AgNMs combined with Raman probes have been reviewed for enhancing Raman signals of probe molecules for quantifying the virus. It also reviews progress made recently in the design of certain specific Raman probe molecules capable of imparting characteristic SERS response/tags for SARS-CoV-2 detection.</p>","PeriodicalId":2,"journal":{"name":"ACS Applied Bio Materials","volume":" ","pages":""},"PeriodicalIF":4.6,"publicationDate":"2024-12-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142811401","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Sensitive Detection of Hg²⁺ and l-Cysteine through Optical Asymmetry-Tuned Fluorescence Switch Off-On Behavior in N-Doped Chiral Carbon Dot.","authors":"Angana Bhattacharya, Dipanjan Samanta, Manisha Shaw, Md Abdus Salam Shaik, Rajarshi Basu, Imran Mondal, Amita Pathak","doi":"10.1021/acsabm.4c01416","DOIUrl":"https://doi.org/10.1021/acsabm.4c01416","url":null,"abstract":"<p><p>Blue-emissive nitrogen-doped chiral carbon dots (d-NCD230 and l-NCD230) exhibiting antipodal chiroptical activity, synthesized from the thermal pyrolysis of citric acid and d/l-aspartic acid in 1:2 molar ratios, have been explored as chirality-based fluorescent turn-off/on probes for the detection of Hg²⁺ and l-cysteine (l-Cys). Circular dichroism (CD) spectroscopy revealed that the chiroptical activity originates from a synergy among intrinsic chirality, chiral precursors on the NCD surface, and hybridization of lower energy levels within the embedded chiral chromophore. Quantitative analysis of optical asymmetry using the Kuhn asymmetry factor (<i>g</i>) at the CD signal of 312 nm showed a higher value for d-NCD230 (1.03 × 10^-4) compared to l-NCD230 (1.13 × 10^-5). Moreover, we have demonstrated chirality transfer and chiral inversion phenomena in d/l-NCDs by preparing carbon dots with different precursor ratios at different temperatures and probing them through CD spectroscopy. The NCDs exhibited selective fluorescence quenching in the presence of Hg²⁺, demonstrating linearity in the Stern-Volmer plot. Limits of detection (LODs) for Hg²⁺ were calculated to be 129 and 192 nM for d-NCD230 and l-NCD230, respectively, in the 0-150 μM concentration range. The quenching mechanism involves nonradiative electron transfer due to Hg²⁺ binding to oxygen-rich functional groups on the d/l-NCD230 surface. The slight variation in LOD values between d-NCD230 and l-NCD230 indicates the negligible effect of the chirality on Hg²⁺ sensing. Notably, the fluorescence intensity of d/l-NCD230 could be restored upon adding l-cysteine, with d-NCD230 showing a more pronounced enhancement than l-NCD230. This differential response is attributed to a preferential stereoselective interaction arising from the homochirality of d-NCD230/Hg²⁺ and l-cysteine. These findings demonstrate the potential of chiral nitrogen-doped carbon dots as sensitive and selective probes for Hg²⁺ and l-cysteine, with implications for environmental monitoring and biological sensing applications.</p>","PeriodicalId":2,"journal":{"name":"ACS Applied Bio Materials","volume":" ","pages":""},"PeriodicalIF":4.6,"publicationDate":"2024-12-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142816640","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Enhancing Performance of Microbial Fuel Cell by Binder-Free Modification of Anode with Reduced Graphene Oxide through One-Step Electrochemical Exfoliation and In Situ Electrodeposition.","authors":"Longxin Li, Xinyuan He, Huahua Li, Yi Lu, Hao Song, Shaoan Cheng","doi":"10.1021/acsabm.4c01502","DOIUrl":"https://doi.org/10.1021/acsabm.4c01502","url":null,"abstract":"<p><p>As the core component of microbial fuel cells, the conductivity and biocompatibility of anode are hard to achieve simultaneously but significantly influence the power generation performance and the overall cost of microbial fuel cells. Stainless steel felt has a low price and high conductivity, making it a potential anode for the large-scale application of microbial fuel cells. However, its poor biocompatibility limits its application. This study provides a one-step binder-free modification method of a stainless steel felt anode with reduced graphene oxide to retain the high conductivity while greatly improving biocompatibility. The maximum power density achieved by reduced graphene oxide modified stainless steel felt was 951.89 mW/m², 5.49 and 1.91 times higher than the unmodified stainless steel felt anode and reduced graphene oxide coated stainless steel felt by Nafion, respectively. The robust reduced graphene oxide modification markedly improved the biocompatibility by forming a uniform biofilm and utilizing the high conductivity of reduced graphene oxide to enhance the charge transfer rate. It led to 92.7 and 37.9% decreases in charge transfer resistance of reduced graphene oxide modified stainless steel felt compared to the unmodified one and the anode modified with reduced graphene oxide by Nafion, respectively. The excellent performance and green synthesis method of the anode validated its potential as a high-performance anode material for scaled-up microbial fuel cell applications.</p>","PeriodicalId":2,"journal":{"name":"ACS Applied Bio Materials","volume":" ","pages":""},"PeriodicalIF":4.6,"publicationDate":"2024-12-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142811398","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Dual Thermo- and pH-Responsive Polymer Nanoparticle Assemblies for Potential Stimuli-Controlled Drug Delivery.","authors":"Sára Pytlíková, Rafal Konefał, Robert Pola, Alena Braunová, Volodymyr Lobaz, Miroslav Šlouf, Hynek Beneš, Daniil Starenko, Kateřina Běhalová, Marek Kovář, Tomáš Etrych, Richard Laga, Michal Pechar","doi":"10.1021/acsabm.4c01167","DOIUrl":"https://doi.org/10.1021/acsabm.4c01167","url":null,"abstract":"<p><p>The development of stimuli-responsive drug delivery systems enables targeted delivery and environment-controlled drug release, thereby minimizing off-target effects and systemic toxicity. We prepared and studied tailor-made dual-responsive systems (thermo- and pH-) based on synthetic diblock copolymers consisting of a fully hydrophilic block of poly[<i>N</i>-(1,3-dihydroxypropyl)methacrylamide] (poly(DHPMA)) and a thermoresponsive block of poly[<i>N</i>-(2,2-dimethyl-1,3-dioxan-5-yl)methacrylamide] (poly(DHPMA-acetal)) as drug delivery and smart stimuli-responsive materials. The copolymers were designed for eventual medical application to be fully soluble in aqueous solutions at 25 °C. However, they form well-defined nanoparticles with hydrodynamic diameters of 50-800 nm when heated above the transition temperature of 27-31 °C. This temperature range is carefully tailored to align with the human body's physiological conditions. The formation of the nanoparticles and their subsequent decomposition was studied using dynamic light scattering (DLS), transmission electron microscopy (TEM), isothermal titration calorimetry (ITC), and nuclear magnetic resonance (NMR). ¹H NMR studies confirmed that after approximately 20 h of incubation at pH 5, which closely mimics tumor microenvironment, approximately 40% of the acetal groups were hydrolyzed, and the thermoresponsive behavior of the copolymers was lost. This smart polymer response led to disintegration of the supramolecular structures, possibly releasing the therapeutic cargo. By tuning the transition temperature to the values relevant for medical applications, we ensure precise and effective drug release. In addition, our systems did not exhibit any cytotoxicity against any of the three cell lines. Our findings underscore the immense potential of these nanoparticles as eventual advanced drug delivery systems, especially for cancer therapy.</p>","PeriodicalId":2,"journal":{"name":"ACS Applied Bio Materials","volume":" ","pages":""},"PeriodicalIF":4.6,"publicationDate":"2024-12-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142811397","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Layered Double Hydroxide (LDH)-Based Nanotripod for High-Entropydynamic Therapy Associated with Metabolism Homeostasis.","authors":"Kai Song, Xueting Yang, Yingying Ren, Zheng Mo, Yu Fei, Xiangling Gu, Shizhuo Xiao, Chenghua Sun, Shanyue Guan, Pengtao Bao, Xiaozhong Qu","doi":"10.1021/acsabm.4c01745","DOIUrl":"https://doi.org/10.1021/acsabm.4c01745","url":null,"abstract":"<p><p>Multielemental transition metal compounds represent a class of promising candidates for the biomedical field due to their unique structure and biomedical application potential. However, their synthesis process remains challenging, which was subject to the high-temperature treatment of the multimetallic elements integrated within one system. Herein, for the first time, we have fabricated the nanotripod, <i>i.e.</i>, (FeCoNiCuZnAl)O_<i>x</i> (denoted as HEO) agent, via the structural topotactic transformation of layered double hydroxide (LDH) precursors with the tunable disorder degree, for highly efficient high-entropydynamic therapy associated with metabolism homeostasis. By virtue of this unique high-entropy structure, the outburst reactive oxygen species (ROS) generation can be regulated via turbulence. These unique high-entropy oxides not only presented outstanding ROS generation efficiency but also broke the intracellular metabolic balance cycle (NADH/NAD⁺) by NO<i>_x</i>-like activity, which can disturb the tumor energy metabolism homeostasis, leading to cell apoptosis. Furthermore, <i>in vitro</i> and <i>in vivo</i> experiments both indicate that this agent was a satisfying candidate for magnetic resonance imaging (MRI)-guided therapy. The findings offer a strategy for the development of high-entropydynamic therapy.</p>","PeriodicalId":2,"journal":{"name":"ACS Applied Bio Materials","volume":" ","pages":""},"PeriodicalIF":4.6,"publicationDate":"2024-12-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142811399","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Biomimetic Approach for Optimal Designing of the Shape and Controlled Release of Therapeutics from Tricompartmental Microcarriers for Managing Parkinson's Disease.","authors":"Nidhi Gupta, Debarghya Saha, Vikramsingh Thakur, Shreyash Santosh Yadav, Sandeep Jat, Pramod Kumar, Ashok Kumar Datusalia, Bhabani K Satapathy, Sampa Saha","doi":"10.1021/acsabm.4c01149","DOIUrl":"https://doi.org/10.1021/acsabm.4c01149","url":null,"abstract":"<p><p>Inspired by the intricate cellular morphology and the discoid shape of red blood cells (RBCs), biomimetic tricompartmental microcarriers (TCM) with controlled release profiles were engineered using an electrohydrodynamic-<i>co</i>-jetting technique for efficient management of Parkinson's disease (PD). While jetting, Levodopa (LD), CD (Carbidopa), and ENT (Entacapone) (3 PD drugs) were directly encapsulated in the three individual compartments of the TCM used for oral administration. The optimal shape and controlled release profiles were obtained by employing the Taguchi orthogonal L9 design-of-experiment approach by systematically varying the processing parameters, i.e., solvent ratio, polymer concentration, and flow rate. The \"smaller-the-better\" norm for the S/N ratio demonstrated the solvent ratio (DMF content) and polymer concentration as the most influential parameters in ensuring the RBC shape and controlling the release of drugs. Analysis of variance and response surface methodology approach provided insights into the optimal influence of control factors on the response variables. Confirmation experiments further validated the optimized microparticles (<i>P</i>_optimized), demonstrating an error of only ∼0.13% in aspect ratio deviation (AR_DEV) and ∼19% (within the tolerance limit) in release factor (RF) from the predicted experiment. Moreover, <i>P</i>_optimized exhibits ∼100% encapsulation efficiency of all three PD drugs, with the cumulative release of ∼100% LD, ∼97% CD, and ∼65% ENT within 5 h of the <i>in vitro</i> study. In addition, <i>in vivo</i> studies such as pharmacokinetics (using healthy rats) and pharmacodynamics [using the MPTP (methyl-4-phenyl-1,2,3,6-tetrahydropyridine)-injected PD-induced mice model] showed that the TCM can effectively control the release of LD (primary drug) for a prolonged period, thereby promising sustained drug delivery and improved therapeutics outcomes.</p>","PeriodicalId":2,"journal":{"name":"ACS Applied Bio Materials","volume":" ","pages":""},"PeriodicalIF":4.6,"publicationDate":"2024-12-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142811396","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Correction to \"Stable Porous Organic Cage Nanocapsules for pH-Responsive Anticancer Drug Delivery for Precise Tumor Therapy\".","authors":"Yanping Deng, Zhenhong Du, Shunfu Du, Nan Li, Wenjing Wang, Kongzhao Su, Daqiang Yuan","doi":"10.1021/acsabm.4c01791","DOIUrl":"https://doi.org/10.1021/acsabm.4c01791","url":null,"abstract":"","PeriodicalId":2,"journal":{"name":"ACS Applied Bio Materials","volume":" ","pages":""},"PeriodicalIF":4.6,"publicationDate":"2024-12-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142805521","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Membrane-Bound Bisindolyl-Based Chromogenic Probes: Analysis of Cyanogenic Glycosides in Agricultural Crops for Possible Remediation.","authors":"Harshal V Barkale, Nilanjan Dey","doi":"10.1021/acsabm.4c00856","DOIUrl":"https://doi.org/10.1021/acsabm.4c00856","url":null,"abstract":"<p><p>Cyanogenic glycosides are plant-derived, nitrogen-containing secondary metabolites that release toxic cyanide ions upon hydrolysis by glycosidic enzymes. Therefore, consuming food items enriched with such compounds without proper remediation can cause acute cyanide intoxication. Thus, in this work, we utilize cyanide-responsive oxidized bisindole-based chromogenic probes to detect cyanogenic glycosides, such as amygdalin and linamarin (LOD: 0.12 μM), in phospholipid membranes. The bilayer surface, owing to its distinct microenvironment, enhances both the sensitivity and specificity of the probes toward amygdalin. The chromogenic response (red to yellow) is influenced by the nature of the lipid membrane (order, polarity, and interfacial hydration) as well as the number of bis-indolyl units in the probe molecules. Semiquantitative analysis of food samples before and after cooking revealed that soaking in water at room temperature significantly reduces the cyanogenic glycoside content. The ability to directly detect cyanogenic glycosides in food samples without pretreatment is a notable aspect of this investigation.</p>","PeriodicalId":2,"journal":{"name":"ACS Applied Bio Materials","volume":" ","pages":""},"PeriodicalIF":4.6,"publicationDate":"2024-12-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142826535","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Glycyrrhizic Acid-Loaded Poloxamer and HPMC-Based <i>In Situ</i> Forming Gel of Acacia Honey for Improved Wound Dressing: Formulation Optimization and Characterization for Wound Treatment.","authors":"Bhawana Jha, Ankit Majie, Kankan Roy, Wei Meng Lim, Bapi Gorain","doi":"10.1021/acsabm.4c01212","DOIUrl":"https://doi.org/10.1021/acsabm.4c01212","url":null,"abstract":"<p><p>The present study aims to formulate a stimuli-responsive <i>in situ</i> hydrogel system to codeliver acacia honey and glycyrrhizic acid for topical application that will aid in absorbing wound exudates, control microbial infestation, and produce angiogenic and antioxidant effects to accelerate wound healing. Therefore, both the natural active constituents were incorporated within an <i>in situ</i> hydrogel composed of poloxamer and hydroxypropyl methylcellulose (HPMC), where the concentrations of the polymers were optimized using Design-Expert software considering optimum values of the dependent variables, gelation temperature (34-37 °C), gelation time (<10 min), and the viscosity (2000-3500 cPs). The optimized formulation showed improved physicochemical properties such as mucoadhesiveness, porosity, swelling, and spreadability, which makes it suitable for wound application. Additionally, the <i>in situ</i> hydrogel exhibited potent <i>in vitro</i> and <i>ex vivo</i> antioxidant effects, <i>in vitro</i> antimicrobial activities, and <i>ex ovo</i> angiogenic effects. Furthermore, the optimized formulation was found to be nontoxic while tested in the HaCaT cell line and acute dermal irritation and corrosion study. The findings of the <i>in vivo</i> wound-healing studies in experimental animal models showed complete wound closure within 15 days of treatment and accelerated development of the extracellular matrix. In addition, the antioxidant, antimicrobial, angiogenic, and wound-healing properties of acacia honey and glycyrrhizic acid coloaded <i>in situ</i> hydrogel were also found to be promising when compared to the standard treatments. Overall, it can be concluded that the optimized stimuli-responsive <i>in situ</i> hydrogel containing two natural compounds could be an alternative to existing topical formulations for acute wounds.</p>","PeriodicalId":2,"journal":{"name":"ACS Applied Bio Materials","volume":" ","pages":""},"PeriodicalIF":4.6,"publicationDate":"2024-12-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142805522","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}