ACS Applied Bio Materials最新文献

{"title":"Dual-Functional Amino-MOF with Enhanced Ice Suppression and Intrinsic Photothermal Activity for High-Performance Oocyte Cryopreservation.","authors":"Pan Wang, Jixiang Zhang, Minghui Zhan, Zicong Tan, Chao Wang, Bianhua Liu, Zhenyang Wang, Jun Zhao","doi":"10.1021/acsabm.5c00517","DOIUrl":"10.1021/acsabm.5c00517","url":null,"abstract":"<p><p>The current vitrification-based oocyte cryopreservation relies on inherently cytotoxic high-concentration cryoprotective agents (CPAs), leading to suboptimal post-thaw survival rates and diminished developmental competence. To address this, we developed a series of highly biocompatible amino-functionalized iron-based metal-organic frameworks (Fe-MOFs) via a facile hydrothermal method to synergistically suppress ice crystallization and minimize the reliance on toxic CPAs. Among the synthesized materials, 2NH₂-MIL88(Fe) demonstrated exceptional ice-inhibition performance, reducing ice crystal size to 16.78% of that observed in pure water, significantly outperforming its nonaminated counterpart MIL88(Fe) and monoaminated counterpart NH₂-MIL88(Fe). Notably, 2NH₂-MIL88(Fe) exhibited an unprecedented intrinsic photothermal response without requiring photosensitizer modification, achieving rapid temperature elevation from 25 to 86.6 °C under 808 nm laser irradiation (1 W/cm², 1 min). When applied to mouse oocyte cryopreservation, this dual-functional amino-MOF enabled complete elimination of dimethyl sulfoxide (DMSO) from CPAs while maintaining an exceptional post-thaw survival rate of 95.1%. Our findings establish a dual correlation between amino group density and both ice-growth suppression efficiency and photothermal performance, revealing a synergistic mechanism for mouse oocyte cryopreservation enhancement. This structure-function relationship provides critical insights for the rational design of next-generation nano-CPAs.</p>","PeriodicalId":2,"journal":{"name":"ACS Applied Bio Materials","volume":" ","pages":"4383-4394"},"PeriodicalIF":4.6,"publicationDate":"2025-05-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143951010","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Vacuum Ultraviolet (VUV)-Induced Physicochemical Engineering of Titanium: Enhanced Fibroblast Activity, Redox System, and Glycosaminoglycan Binding for Soft Tissue Integration.","authors":"Keiji Komatsu, Takanori Matsuura, Toshikatsu Suzumura, Rune Shibata, Po-Chun Chen, Takahiro Ogawa","doi":"10.1021/acsabm.5c00283","DOIUrl":"10.1021/acsabm.5c00283","url":null,"abstract":"<p><p>Bacterial invasion at the titanium-tissue interface causes peri-implant inflammation, posing challenges for implants in orthopedics, maxillofacial prosthetics, and dentistry. This study hypothesized that titanium surface decarbonization improves soft tissue cell adhesion and growth. One-minute vacuum ultraviolet (VUV) light treatment at 172 nm reduced surface carbon from 60% to 29% without altering surface topography, making surfaces hydrophilic and hydro-attractive. Human fibroblasts attached to VUV-treated surfaces 2-4 times more frequently than untreated surfaces, with an even greater increase on tilted and curved surfaces. Fibroblast proliferation rose 2-6 times, with an expedited G1-to-S phase transition. Cell retention under dislodging forces increased 2-5 times on VUV-treated surfaces. RNA sequencing showed upregulation of extracellular matrix production, growth factors, cell cycle progression, antioxidant defenses, and proteoglycan/glycosaminoglycan (GAG)-binding, alongside downregulation of the inflammatory response on VUV-treated titanium surfaces. An oxidative stress test showed minimal adverse effects from hydrogen peroxide on cells on VUV-treated surfaces, attributed to increased intracellular glutathione reserves. Enhanced adhesion on VUV-treated titanium was negated by treating the cells with GAG-cleaving enzymes. These findings demonstrate that VUV-mediated decarbonization enhances fibroblast attachment, proliferation, and adhesion by fostering homeostatic cellular phenotypes involving proteoglycan/GAG interactions and antioxidant defense, offering a strategy to improve the soft tissue sealing around titanium implants.</p>","PeriodicalId":2,"journal":{"name":"ACS Applied Bio Materials","volume":" ","pages":"4166-4185"},"PeriodicalIF":4.6,"publicationDate":"2025-05-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143951031","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Integration of Carboxylate Carbon Dot-Supported Gold Nanoparticles with Boron Nitride Nanosheets for Electrochemical Sensing of Prostate-Specific Antigen via Sandwich Immunoassays.","authors":"So Eun Kim, Kathannan Sankar, Jae Chol Yoon, Alagan Muthurasu, Hak Yong Kim","doi":"10.1021/acsabm.5c00276","DOIUrl":"10.1021/acsabm.5c00276","url":null,"abstract":"<p><p>An electrochemical sandwich immunoassay was fabricated to measure the prostate-specific antigen (PSA) biomarker. The PSA immunosensor was developed by altering the surface of a glassy carbon electrode (GCE) with a nanocomposite comprising carboxyl-functionalized carbon dots that support gold nanoparticles (AuNPs), which were then combined with hydroxylated boron nitride nanosheets (HO-BN). This construction possesses distinctive signal enhancement characteristics and was prepared via a facile method. Considering the high biological affinity of AuNPs for biomolecules, carbon dots (CDs) with carboxyl (COOH) groups make a suitable substrate for electrode modification. This alteration allows for the PSA antibody (Ab1) attachment, resulting in a sandwich-like configuration. Additionally, carbon dot-stabilized AuNPs integrated into HO-BN nanosheet nanocomposites considerably boost the electron transfer rate, leading to remarkable potential in sensor applications. By utilizing horseradish peroxidase (HRP)-conjugated antifree PSA antibody (Ab2), a reduction in hydrogen peroxide (H₂O₂) was achieved within the electrochemical cell. This reduction led to increased current, attributed to the increased PSA concentration, which is required for the biosensor analysis to perform properly. The developed immunosensor revealed a linear correlation with PSA concentrations ranging from 1.0 to 3500 pg mL^-1, achieving a low detection limit of 0.136 pg mL^-1. Furthermore, the PSA aptasensor demonstrates excellent selectivity, remarkable stability, and commendable reproducibility, indicating its significant potential for clinical research and diagnostic applications.</p>","PeriodicalId":2,"journal":{"name":"ACS Applied Bio Materials","volume":" ","pages":"4153-4165"},"PeriodicalIF":4.6,"publicationDate":"2025-05-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143950643","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Manganese-Based Nanotherapeutics for Targeted Treatment of Breast Cancer.","authors":"Mubassir Khan, Razi Ullah, Syed Mubassir Shah, Umar Farooq, Jun Li","doi":"10.1021/acsabm.5c00040","DOIUrl":"10.1021/acsabm.5c00040","url":null,"abstract":"<p><p>Breast cancer (BC) is one of the most common cancers among women and is associated with high mortality. Traditional modalities, including surgery, radiotherapy, and chemotherapy, have achieved certain advancements but continue to combat challenges including harm to healthy tissues, resistance to treatment, and adverse drug reactions. The rapid advancements in nanotechnology recently facilitated the exploration of innovative strategies for breast cancer therapy. Manganese-based nanotherapeutics have attracted great attention because of their unique characteristics such as tunable structures/morphologies, versatility, magnetic/optical properties, strong catalytic activities, excellent biodegradability, and biocompatibility. In this review, we highlighted different types of Mn-based nanotherapeutics to modulate TME, including metal-immunotherapy, alleviating tumor hypoxia, and increasing reactive oxygen species production, and we emphasized its role in magnetic resonance imaging (MRI)-guided therapy, photoacoustic imaging, and theranostic-based therapy along with a therapeutic carrier, all of which were discussed in the context of breast cancer. Hopefully, the present review will provide insights into the current landscape and future directions of multifunctional applications of Mn-based nanotherapeutics in the field of breast cancer treatment.</p>","PeriodicalId":2,"journal":{"name":"ACS Applied Bio Materials","volume":" ","pages":"3571-3600"},"PeriodicalIF":4.6,"publicationDate":"2025-05-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12094176/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143950647","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Carboxymethyl Chitosan Capped Bimetallic Nanoparticles Entrapped in Theranostic Nanofibers: Antimicrobial Peptide Coating, In Vitro, In Vivo Characterization for MDR Microbial Infection and Photoacoustic/Optical Imaging.","authors":"Ankit Kumar Malik, Aseem Setia, Dipti Verma, Matte Kasi Viswanadh, Ashim Mukherjee, Madaswamy S Muthu","doi":"10.1021/acsabm.4c01883","DOIUrl":"10.1021/acsabm.4c01883","url":null,"abstract":"<p><p>Wound dressings, integrated with nanotechnology, have garnered considerable attention recently due to their ability to synergistically combine antimicrobial efficacy with wound healing properties, while also supporting adherence to standardized wound care protocols. We have developed smart theranostic wound dressings composed of carboxymethyl chitosan coated gold-silver-LL37 nanoparticles (G-S-CMC-Pep-NPs), of 155.1 ± 11.2 nm in size and a charge over the surface of +34.6 ± 3.7 mV. The optimized G-S-CMC-Pep-NPs were observed to exhibit minimal inhibitory and bactericidal concentration in the range of 0.390-0.781 μg/mL, also illustrated the maximum zone of inhibition (ZOI) of 21.61 ± 1.06 and 18.85 ± 1.22 mm, toward multidrug resistant (MDR) bacteria of <i>P. aeruginosa</i> and <i>S. aureus</i> respectively. TEM analysis of the microbial cells post-12-h treatment revealed irregularly undulating and disrupted cell walls, loss of cell wall integrity, and evidence of DNA condensation. Additionally, hemolysis assays demonstrated that G-S-CMC-Pep-NPs exhibited a nonhemolytic profile when tested on rodent blood, indicating their excellent biocompatibility. Furthermore, G-S-CMC-Pep-NPs were uniformly integrated into chitosan poly(vinyl alcohol) nanofibers (G-S-CMC-Pep-NPs-NFs) having a size ranging from 100 to 350 nm, resulting in an antimicrobial wound dressing, when applied to microbial-infected wounds in mice, achieved a 92.4% wound closure rate within 12 days of treatment. Additionally, this study is further substantiated through the analysis of wound marker protein expression levels, along with in vivo optical and ultrasound/photoacoustic imaging. The ultrasound/photoacoustic imaging offered an in-depth evaluation of the complex wound healing mechanism, enabling real-time visualization, high-resolution spatial imaging, and precise assessment of blood flow dynamics.</p>","PeriodicalId":2,"journal":{"name":"ACS Applied Bio Materials","volume":" ","pages":"3762-3782"},"PeriodicalIF":4.6,"publicationDate":"2025-05-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143950797","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Crystal Violet-Loaded Bi(III)-Based Metal-Organic Framework Boosting Enhanced Photothermal Effect for Breast Cancer Treatment.","authors":"Xue Wu, Shun Yang, Mingyu Li, Jingyi Zhang, Xiuxiu Wang, Bing Shi, Jing Zhao, Yue Zhao","doi":"10.1021/acsabm.5c00129","DOIUrl":"10.1021/acsabm.5c00129","url":null,"abstract":"<p><p>Recently, metal-organic frameworks (MOFs) have emerged as promising platforms to fabricate photothermal therapeutic materials to cure tumors, but the toxic heavy metals in many MOFs may pose great threats to normal cells. In consideration of the low toxicity of bismuth, herein we made great efforts to synthesize porous bismuth-based MOFs (Bi-MOFs) to construct photothermal conversion materials for cancer treatment. By the reaction of 4,4',4''-tricarboxylic triphenylamine (H₃TCA) and Bi(NO₃)₃ under solvothermal conditions, a porous Bi-MOF was obtained with the formula of {[Bi₂(TCA)₂(H₂O)₃]·6DMF·21H₂O}_<i>n</i> (<b>Bi-TCA</b>). Structural analysis revealed that complex <b>Bi-TCA</b> displayed a three-dimensional (3D) coordination framework featuring abundant accessible channels and cages with a porosity of 73.3% according to the calculating results of PLATON. Due to the high porosity, complex <b>Bi-TCA</b> could effectively encapsulate the dye crystal violet (CV) into the voids and the resulting dye-loaded composite <b>CV@Bi-TCA</b> exhibited extended red-shifted light-absorbing property and enhanced photothermal conversion capability under 455 nm laser irradiation. Further anticancer experiments demonstrated that composite <b>CV@Bi-TCA</b> could efficiently inhibit the growth of breast cancer cells both in vitro and in vivo under 455 nm laser irradiation. This work may promote investigations on the low-toxicity Bi-MOFs for photothermal cancer therapy.</p>","PeriodicalId":2,"journal":{"name":"ACS Applied Bio Materials","volume":" ","pages":"3972-3982"},"PeriodicalIF":4.6,"publicationDate":"2025-05-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143950806","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Curcumin-Loaded Silver-Based Metal-Organic Frameworks: Efficient Antibacterial and Antioxidant Properties against <i>Escherichia coli</i> and <i>Staphylococcus aureus</i> for Promoting Infected Wound Healing.","authors":"Jinyan Du, Jinrui Hou, Shiji Liu, Xinyue Wu, Liangde Hu, Wenjiang Xu, Shujuan Zhuo","doi":"10.1021/acsabm.5c00275","DOIUrl":"10.1021/acsabm.5c00275","url":null,"abstract":"<p><p>Slow or noticeably delayed wound healing is frequently intimately linked to bacterial infection and excessive reactive oxygen species (ROS), while the inappropriate usage of antibiotics fuels the rise of bacterial resistance. The innovative materials are desperately needed to eliminate bacteria and effectively accelerate wound healing. In this work, a curcumin-loaded silver-based metal-organic framework (Cur/Ag-MOF) composite nanomaterial was developed, which exhibited good antimicrobial activity, biocompatibility, and drug resistance. Meanwhile, surface-loaded curcumin can effectively eliminate excess free radicals and promote wound healing due to its antioxidative and ROS scavenging properties. Additionally, it was discovered that the application of Cur/Ag-MOF to the site of skin trauma significantly sped up the process of wound closure in mice used as subjects. These findings highlighted its great potential for treating bacterial infection-induced skin injuries and aiding the healing and reconstruction of skin tissues.</p>","PeriodicalId":2,"journal":{"name":"ACS Applied Bio Materials","volume":" ","pages":"4140-4152"},"PeriodicalIF":4.6,"publicationDate":"2025-05-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143950910","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Biomimetic Polythiophene: g-C₃N₄ Nanotube Composites with Induced Creatinine and Uric Acid Specificity for Portable CKD and Gout Detection.","authors":"Zohaib Saddique, Noor Shahzad, Maleeha Saeed, Adeel Afzal","doi":"10.1021/acsabm.5c00371","DOIUrl":"10.1021/acsabm.5c00371","url":null,"abstract":"<p><p>Despite significant advancements in disease diagnostics, the development of portable, highly selective, and low-cost electrochemical sensors for real-time, noninvasive detection of chronic kidney disease (CKD) and gout biomarkers remains a challenge. In this work, we demonstrate an inexpensive CKD and gout diagnostic platform with the incorporation of biomimetic polymer matrix nanocomposites based on creatinine (CRE) and uric acid (UA) imprinted conducting polythiophene (MIP) and graphitic carbon nitride nanotubes (NTs). These nanocomposites are termed CRE-MIPNT or UA-MIPNT, depending on the template. Disposable electrochemical devices are fabricated by anchoring CRE-MIPNT or UA-MIPNT nanocomposites on screen-printed Au microelectrodes. The surface micrographs exhibit the integration of NTs in the polymer matrix, resulting in highly leptokurtic surfaces. Consequently, the charge transfer resistance at the electrode-electrolyte interface is significantly reduced, as characterized by electrochemical impedance spectroscopy. An increase in the electroactive surface area and charge transfer kinetics is also observed for the CRE-MIPNT/Au-SPE and UA-MIPNT/Au-SPE sensors. In comparison to nonimprinted sensors, their performance is investigated in terms of their voltammetric response toward various biomarker concentrations in standard redox solutions. The sensitivity in the CV measurements is 0.59 μA cm^-2 nM^-1 for CRE-MIPNT/Au-SPE and 0.78 μA cm^-2 μM^-1 for UA-MIPNT/Au-SPE sensors, with detection limits of 390 pM and 162 nM for CRE and UA, respectively. The sensors demonstrate high selectivity toward the target analytes while showing minimal interference from other metabolites. Moreover, recovery rates for spiked saliva samples ranged between 97 and 102%, which indicates the reliability of the sensor for real-world applications.</p>","PeriodicalId":2,"journal":{"name":"ACS Applied Bio Materials","volume":" ","pages":"4262-4271"},"PeriodicalIF":4.6,"publicationDate":"2025-05-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143950995","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Tailoring Alkyl Side Chains of Ionizable Amino-Polyesters for Enhanced In Vivo mRNA Delivery.","authors":"Aida López Espinar, Lianne M Mulder, Mohamed Elkhashab, Zahra Khan, Mariusz Czarnocki-Cieciura, Maria R Aburto, Sonja Vucen, Piotr S Kowalski","doi":"10.1021/acsabm.5c00116","DOIUrl":"10.1021/acsabm.5c00116","url":null,"abstract":"<p><p>Lipid nanoparticles (LNPs) containing ionizable lipids are the most clinically advanced platform for mRNA delivery, but their application beyond the liver remains challenging. Polymer-lipid hybrid nanoparticles offer a promising alternative, combining the synthetic versatility and unique properties of polymers with the biocompatibility of lipid excipients. While the significance of alkyl tail design is well-recognized for ionizable lipids, the impact of the polymer side chain composition on interactions with lipid excipients, mRNA delivery efficacy, and tissue specificity remains poorly understood. Here, we focus on a class of ionizable amino-polyesters (APEs) that exhibit features desired for potential clinical applications, including narrow molecular weight distribution and a good safety profile, and investigate the effect of polymer side chain composition on the formulation of APE lipid nanoparticles (APE-LNPs) for mRNA delivery. A library of 36 APEs was synthesized via ring-opening polymerization of chemically diverse tertiary amino-alcohols and lactone monomers with distinct alkyl side chain compositions, including variations in length and unsaturation. We show that optimal alkyl side chain length is critical for the assembly of stable mRNA nanoparticles and efficient mRNA delivery both <i>in vitro</i> and <i>in vivo</i>. Top-performing APE-LNPs display superior delivery efficacy <i>in vitro</i> and in extrahepatic tissues compared to benchmark LNPs, including DLin-MC3-DMA ionizable lipid. The polymer chain composition affects the tissue selectivity of APE-LNPs, with shorter side chains (4-5 carbons) effectively targeting the spleen and lungs, while longer chains (7-9 carbons) show enhanced liver delivery. We also explored the relevance of lipid excipients in APE-LNPs, demonstrating the essential role of unsaturated phospholipids in enhancing cellular uptake and mRNA delivery, and the limited relevance of cholesterol. These findings provide valuable insights into the design of polymers for use in the LNP context, which could aid the development of polymeric alternatives to ionizable lipids and expand the utility of mRNA LNP technology to nonliver tissues.</p>","PeriodicalId":2,"journal":{"name":"ACS Applied Bio Materials","volume":" ","pages":"3958-3971"},"PeriodicalIF":4.6,"publicationDate":"2025-05-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12093369/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143951060","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Electrochemical Immunosensor for C-Reactive Protein Detection Using an Optimized Bioinspired Ni-Doped Copper Ferrite Nanocomposite Interface for Cardiovascular Disease Management.","authors":"Astha Singh, Siddhima Singh, Neelottma Singh, Jay Singh","doi":"10.1021/acsabm.5c00406","DOIUrl":"https://doi.org/10.1021/acsabm.5c00406","url":null,"abstract":"<p><p>Currently, cardiovascular diseases (CVDs) are the leading cause of death globally. According to the WHO, the number of CVDs-related deaths increases by 20% annually. C-reactive protein (CRP), a biomarker for CVDs that sharply rises during cardiac events, was used to monitor these critical conditions at an early stage. To address the need for highly sensitive, selective, and portable CRP detection, a nickel-doped copper ferrite nanoparticle-based electrochemical biosensor was developed. In this work, optimized nickel-doped copper ferrite (Ni_(0.10)CuFe₂O₄) nanoparticles (NPs) were synthesized by doping copper ferrite NPs with varying nickel concentrations (0.05, 0.10, 0.15, and 0.20 M) via a hydrothermal method using aqueous leaf extract of <i>Magnolia grandiflora</i>. The NPs were characterized by using XRD, FT-IR, UV, SEM, EDX, and HR-TEM/TEM. The synthesized NPs were deposited on a functionalized indium tin oxide (ITO) surface via the electrophoretic deposition (EPD) method. Electrochemical sensing was performed by using electrochemical impedance spectroscopy (EIS). The biosensor demonstrated linear detection of CRP biomarkers across a range of 0.5 to 500 ng/mL, with a sensitivity of 5.426 Ω/ng cm² at 0.5 ng/mL and 0.68244 Ω/ng cm² at 500 ng/mL. The limit of detection (LOD) values were 0.038 ng/mL (lower concentration range) and 0.033 ng/mL (higher concentration of the analyte range).</p>","PeriodicalId":2,"journal":{"name":"ACS Applied Bio Materials","volume":" ","pages":""},"PeriodicalIF":4.6,"publicationDate":"2025-05-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144100956","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}