ACS Applied Bio Materials最新文献

{"title":"Copper-Based Metal-Organic Framework as a Potential Therapeutic Gas Carrier: Optimization, Synthesis, Characterization, and Computational Studies.","authors":"Chitrangda Singh, Chandan Bhogendra Jha, Avnika Singh Anand, Ekta Kohli, Neha Manav, Raunak Varshney, Sreedevi Upadhyayula, Rashi Mathur","doi":"10.1021/acsabm.4c01907","DOIUrl":"10.1021/acsabm.4c01907","url":null,"abstract":"<p><p>The broad spectrum of health conditions and the global pandemic, leading to inadequate medical oxygen supply and management, has driven interest in developing porous nanocarriers for effective oxygenation strategies. We aim to develop an injectable oxygen carrier with regard to biocompatibility, safety, prehospital availability, and universal applicability. In this study, we have tried to identify important functional sites on metal-organic frameworks (MOFs) for gas binding with the help of Grand canonical Monte Carlo simulation. We have synthesized a copper-based MOF (Cu-BTC) with a 1,3,5-benzenetricarboxylic acid linker through a solvothermal approach as a competent porous adsorbent for oxygen storage and delivery. To optimize process variables, we performed statistical analysis using response surface methodology. A quadratic model was developed to study the interaction between independent variables and the response (i.e., maximizing surface area), whose adequacy is validated by the correlation between experimental and predicted values using the ANOVA method. The synthesized Cu-BTC, before and after oxygen loading, was characterized using X-ray diffraction, surface area, along with pore distribution measurement, particle size analysis, scanning electron microscopy, transmission electron microscopy, and gas adsorption studies. The Cu-BTC MOF exhibited an oxygen uptake of 4.6 mmol g^-1, the highest among all the oxygen carriers reported in the literature under the same operating conditions. Overall, our findings suggest that this synthesized Cu-BTC with high surface area (1389 m² g^-1), high porosity, optimum oxygen uptake, and good biocompatibility would show potential toward efficient storage and delivery (direct to the targeted site) of medical oxygen to raise the blood oxygen saturation level.</p>","PeriodicalId":2,"journal":{"name":"ACS Applied Bio Materials","volume":" ","pages":"2440-2458"},"PeriodicalIF":4.6,"publicationDate":"2025-03-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143456236","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Innovative Plant Exosome Delivery System for Enhancing Antiaging Potency on Skin.","authors":"Wei Wei, Xiaobing Ren, Fan Yi, Xuhui Zhang, Jiali Hou, Zhenxing Zhang, Liqian Yuan, Li Li, Qi Gao","doi":"10.1021/acsabm.4c01691","DOIUrl":"10.1021/acsabm.4c01691","url":null,"abstract":"<p><p>Plant exosomes, small vesicles released by plant cells that contain various bioactive molecules, have garnered great attention for their potential applications in medicine, yet their delivery applications for skincare remain underexploited. Resveratrol (RES), renowned for its remarkable antiaging properties, faces challenges in transdermal absorption due to the stratum corneum barrier, hindering its efficacy. To address this, we developed a delivery system incorporating RES-loaded plant (<i>Leontopodium alpinum</i>) exosome (<i>LEO</i>EXO@RES). Evaluation through both <i>in vitro</i> and <i>in vivo</i> experiments demonstrated <i>LEO</i>EXO@RES's enhanced delivery of bioactive compounds and multifaceted skincare impact. Specifically, this approach not only promoted environmentally responsible waste reuse but also achieved harmonious synergy between <i>LEO</i>EXO carriers and RES, enhancing their overall efficacy. The <i>LEO</i>EXO@RES effectively ameliorated inflammation levels and inhibited cellular senescence, highlighting its promising potential in antiaging skincare applications.</p>","PeriodicalId":2,"journal":{"name":"ACS Applied Bio Materials","volume":" ","pages":"2117-2127"},"PeriodicalIF":4.6,"publicationDate":"2025-03-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143481658","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Structure Characterization of Bacterial Microcompartment Shells via X-ray Scattering and Coordinate Modeling: Evidence for Adventitious Capture of Cytoplasmic Proteins.","authors":"Xiaobing Zuo, Alexander Jussupow, Nina S Ponomarenko, Thomas D Grant, Nicholas M Tefft, Neetu Singh Yadav, Kyleigh L Range, Corie Y Ralston, Michaela A TerAvest, Markus Sutter, Cheryl A Kerfeld, Josh V Vermaas, Michael Feig, David M Tiede","doi":"10.1021/acsabm.4c01621","DOIUrl":"10.1021/acsabm.4c01621","url":null,"abstract":"<p><p>Bacterial microcompartments (BMCs) are self-assembling protein shell structures that are widely investigated across a broad range of biological and abiotic chemistry applications. A central challenge in BMC research is the targeted capture of enzymes during shell assembly. While crystallography and cryo-EM techniques have been successful in determining BMC shell structures, there has been only limited success in visualizing the location of BMC-captured enzyme cargo. Here, we demonstrate the opportunity to use small-angle X-ray scattering (SAXS) and pair distance distribution function (PDDF) measurements combined with quantitative comparison to coordinate structure models as an approach to characterize BMC shell structures in solution conditions directly relevant to biochemical function. Using this approach, we analyzed BMC shells from <i>Haliangium ochraceum</i> (HO) that were isolated following expression in <i>E. coli</i>. The analysis allowed the BMC shell structures and the extent of encapsulated enzyme cargo to be identified. Notably, the results demonstrate that HO-BMC shells adventitiously capture significant amounts of cytoplasmic cargo during assembly in <i>E. coli</i>. Our findings highlight the utility of SAXS/PDDF analysis for evaluating BMC architectures and enzyme encapsulation, offering valuable insights for designing BMC shells as platforms for biological and abiotic catalyst capture within confined environments.</p>","PeriodicalId":2,"journal":{"name":"ACS Applied Bio Materials","volume":" ","pages":"2090-2103"},"PeriodicalIF":4.6,"publicationDate":"2025-03-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143522061","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Long-Term and Real-Time Post-External Radiotherapy Assessment Based on an In Situ Activatable Radiolabeled Platform.","authors":"Fang Jia, Qiulian Mao, Jing Liu, Haorong Jiao, Mei Chen, Xinyue Wu, Jiabin Cui","doi":"10.1021/acsabm.4c01913","DOIUrl":"10.1021/acsabm.4c01913","url":null,"abstract":"<p><p>Long-term monitoring in postoperative assessment is essential for clinicians to assess the effectiveness of therapies and establish subsequent clinical pharmacotherapeutic plans. However, precise and real-time postoperative assessment is often overlooked, relying instead on various clinical histopathological and cytological assays or the experience of physicians. Therefore, it is urgent to develop a general strategy for long-term, real-time, and accurate postoperative assessment. Herein, we present a facile method utilizing radiolabeled probes for postradiotherapy assessment. The probe consists of a tumor-specific targeting group, an external radiotherapy-activated peptide sequence (DEVD), and a ¹⁷⁷Lu-1,4,7,10-tetraazacyclododecane-<i>N</i>,<i>N</i>',<i>N</i>″,<i>N</i>‴-tetraacetic acid (DOTA)-decorated tetraphenyl ethylene. This design not only avoids photobleaching and the limitations associated with traditional organic ligands for long-term monitoring but also achieves in situ aggregation at the lesion site, allowing for prolonged tumor retention over 96 h. This work serves as a glance at utilizing radiolabeled probes for postoperative assessment, broadening the possibilities for the design, application, and clinical translation of radionuclide-labeled probes.</p>","PeriodicalId":2,"journal":{"name":"ACS Applied Bio Materials","volume":" ","pages":"2429-2439"},"PeriodicalIF":4.6,"publicationDate":"2025-03-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143389490","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Synthesis of Yttria Nanoparticle-Loaded Electrospun Nanofibers for Enhanced Antimicrobial Activity, Biofilm Inhibition, and Alleviation of Diabetic Wounds.","authors":"Adrija Ghosh, Tuhin Bhattacharya, Debashmita Mandal, Koushik Dutta, Sanjit Dey, Kasturi Saha, Dipankar Chattopadhyay","doi":"10.1021/acsabm.4c01818","DOIUrl":"10.1021/acsabm.4c01818","url":null,"abstract":"<p><p>Diabetes-related sores and ulcers are quite common around the world and can cause complicated disruptions to both patient compliance and socioeconomic structure. Diabetic wounds take longer to heal due to pathophysiological causes, persistent infections, and increasingly severe medical problems. Nanoparticles (NPs) derived from nanotechnology have drawn interest due to their revolutionary potential in understanding the biological milieu and offering therapeutic strategies for wound healing. In this regard, the potential of yttrium oxide nanoparticles (YNPs) has been studied extensively to understand their efficacy in diabetic wound healing. Yttrium oxide nanoparticles having size in the range of 2-10 nm were prepared and incorporated into nanofibrous mats consisting of polyurethane as the matrix polymer, and leaf extract of <i>Azadirachta indica</i> and clindamycin hydrochloride as additive conventional antidiabetic and antibacterial agents to form S3. Physicochemical characterization tests confirmed the formation of nanofibers having average diameters in the range of 320-470 nm, respectively. The study demonstrated that S3 shows an enhanced zone of inhibition against <i>E. coli</i> (29 mm), <i>S. aureus</i> (32 mm), and <i>P. aeruginosa</i> (30 mm). Moreover, the nanofibrous mats also prevented microbial penetration and biofilm formation, as observed from MTT, CV, and confocal microscopy images. <i>In vivo</i> wound healing study conducted on diabetic mice revealed that S3 exhibited high wound contraction after 9 days of treatment. Additionally, the fabricated mat lowered plasma glucose levels, hepatotoxicity, and oxidative stress biomarkers. Therefore, it can be concluded that YNP-loaded nanofibrous composite mats have a strong potential in alleviating diabetic wounds.</p>","PeriodicalId":2,"journal":{"name":"ACS Applied Bio Materials","volume":" ","pages":"2287-2298"},"PeriodicalIF":4.6,"publicationDate":"2025-03-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143513984","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Solvent-Free Microfluidic Fabrication of Antimicrobial Lipid Nanoparticles.","authors":"Marta Ruano, Tun Naw Sut, Sue Woon Tan, Alexander B Mullen, Donald Kelemen, Valerie A Ferro, Joshua A Jackman","doi":"10.1021/acsabm.4c01747","DOIUrl":"10.1021/acsabm.4c01747","url":null,"abstract":"<p><p>Antimicrobial lipid nanoparticles composed of monoglycerides offer a promising strategy to inhibit membrane-enveloped viral and bacterial pathogens. However, previous efforts mainly focused on fabricating nanoparticles from long-chain monoglycerides, which lack intrinsic antimicrobial activity but contribute to nanoparticle stability and structural integrity. In contrast, shorter-chain monoglycerides often exhibit potent antimicrobial effects but do not self-assemble into colloidally stable nanoparticles and lose efficacy upon dilution. To overcome these limitations and incorporate antimicrobial monoglycerides into a stable nanoparticle configuration, we report a solvent-free microfluidic fabrication strategy that combines the functional characteristics of different monoglycerides to prepare interfacially active, monoglyceride-based nanoparticles with mixed compositions that display potent antibacterial activity. Unlike conventional microfluidic mixing methods that rely on volatile organic solvents, our approach utilizes pharmaceutical-grade materials and does not require organic solvent removal, hence eliminating the need for a dialysis step postfabrication. Dynamic light scattering (DLS) and zeta potential measurements verified that the fabricated nanoparticles had ∼250-350 nm diameters and exhibited high colloidal stability whereas the antibacterial activity of the nanoparticles against <i>Staphylococcus aureus</i> bacteria depended strongly on the nanoparticle composition. Nanoparticles composed of glycerol monooleate alone were inactive, while the inclusion of glycerol monolaurate slightly enhanced antibacterial activity. Surprisingly, the further addition of glycerol monobehenate or glycerol dibehenate─previously considered inactive structural components that are used to improve nanoparticle cohesion─boosted antibacterial potency by up to 270-fold. Biophysical experiments showed that nanoparticle compositions with greater antibacterial activity induced more pronounced membrane disruption, as observed in quartz crystal microbalance-dissipation and electrochemical impedance spectroscopy measurements. These findings demonstrate that combining different monoglycerides can significantly enhance the antibacterial activity of lipid-based nanoparticles and underscore the potential of membrane biophysics approaches to guide performance optimization, highlighting the capability to tune membrane-disruptive properties in physiologically relevant pH conditions.</p>","PeriodicalId":2,"journal":{"name":"ACS Applied Bio Materials","volume":" ","pages":"2194-2203"},"PeriodicalIF":4.6,"publicationDate":"2025-03-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143539399","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Transparent Biomaterial-Based Nonvolatile Bioelectronic Memory with Excellent Endurance.","authors":"Dimpal Kumari, Anurag Gupta, Karuna Kumari, Shantanu Majumder, Soumya Jyoti Ray","doi":"10.1021/acsabm.4c01645","DOIUrl":"10.1021/acsabm.4c01645","url":null,"abstract":"<p><p>With the recent upsurge of data-driven technology, the demand for storage elements has pushed the researchers to explore design of nobel nonvolatile memory devices with diverse functionalities. However, the management of electronic waste has become a prominent challenge due to the rapid growth of the solid-state electronics industry. Biomaterial-based Resistive Random Access Memory (Bio-RRAM) has become one of the most promising devices that can augment the quality of memory devices because of their environmentally benign behavior, biocompatible, nontoxic, transient, transferable, flexible, dissolvable, and biodegradable nature. In this work, we report the fabrication of MIM-structured RRAM devices based on two biomaterials, namely, ovalbumin liquid and acemannan polysaccharide gel, as switching layers. Further, they are characterized by several analytical techniques. The electrical transport measurement revealed bipolar resistive switching behavior, sustainable over 1000 consecutive cycles. The devices demonstrated supreme endurance over 1000 switching cycles with a maximum ON/OFF ratio of ∼10²-10³. The switching process can be explained through the formation and rupture of conducting filaments formed by the migration of Ag ions. Design of neuro-memristive synapse has been further been explored to demonstrate various neuromorphic functionalities such as long/short-term potentiation, depression, and plasticity. Due to simultaneous presence of resistive switching with the negative differential resiatance (NDR) effect, remarkable endurance, ease of fabrication, cost reduction, and environmental compatibility, neuromorphic functionalities, the RRAM structures could be of potential interest for bioelectronic memory design, wearable and flexible electronics and neuromorphic computing.</p>","PeriodicalId":2,"journal":{"name":"ACS Applied Bio Materials","volume":" ","pages":"2104-2116"},"PeriodicalIF":4.6,"publicationDate":"2025-03-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143539448","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"From Unprintable Peptidic Gel to Unstoppable: Transforming Diphenylalanine Peptide (Fmoc-FF) Nanowires and Cellulose Nanofibrils into a High-Performance Biobased Gel for 3D Printing.","authors":"Feras Dalloul, J Benedikt Mietner, Dhanya Raveendran, Shouzheng Chen, Enguerrand Barba, Dennis M J Möck, Fabio Hubel, Benedikt Sochor, Sarathlal Koyiloth Vayalil, Linnea Hesse, Andrea Olbrich, Jörn Appelt, Peter Müller-Buschbaum, Stephan V Roth, Julien R G Navarro","doi":"10.1021/acsabm.4c01803","DOIUrl":"10.1021/acsabm.4c01803","url":null,"abstract":"<p><p>The growing interest in gel-based additive manufacturing, also known as three-dimensional (3D) gel-printing technology, for research underscores the crucial need to develop robust biobased materials with excellent printing quality and reproducibility. The main focus of this study is to prepare and characterize some composite gels obtained with a low-molecular-weight gelling (LMWG) peptide called Fmoc-diphenylalanine (Fmoc-FF) and two types of cellulose nanofibrils (CNFs). The so-called Fmoc-FF peptide has the ability to self-assemble into a nanowire shape and therefore create an organized network that induces the formation of a gel. Despite their ease of preparation and potential use in biological systems, unfortunately, those Fmoc-FF nanowire gel systems cannot be 3D printed due to the high stiffness of the gel. For this reason, this study focuses on composite materials made of cellulose nanofibrils and Fmoc-FF nanowires, with the main objective being that the composite gels will be suitable for 3D printing applications. Two types of cellulose nanofibrils are employed in this study: (1) unmodified pristine cellulose nanofibrils (uCNF) and (2) chemically modified cellulose nanofibrils, which ones have been grafted with polymers containing the Fmoc unit on their backbone (CNF-<i>g</i>-Fmoc). The obtained products were characterized through solid-state cross-polarization magic angle-spinning ¹H NMR and confocal laser scanning microscopy. Within these two CNF structures, two composite gels were produced: uCNF/Fmoc-FF and CNF-<i>g</i>-Fmoc/Fmoc-FF. The mechanical properties and printability of the composites are assessed using rheology and challenging 3D object printing. With the addition of water, different properties of the gels were observed. In this instance, CNF-<i>g</i>-Fmoc/Fmoc-FF (<i>c</i> = 5.1%) was selected as the most suitable option within this product range. For the composite bearing uCNF, exceptional print quality and mechanical properties are achieved with the CNF/Fmoc-FF gel (<i>c</i> = 5.1%). The structures are characterized by using field emission scanning electron microscopy (FESEM) and small-angle X-ray scattering (SAXS) measurements.</p>","PeriodicalId":2,"journal":{"name":"ACS Applied Bio Materials","volume":" ","pages":"2323-2339"},"PeriodicalIF":4.6,"publicationDate":"2025-03-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11921036/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143571517","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Bioderived Green Algae Metabolite as a Latent Cross-Linking Agent for Protein-Based Hydrogels with High Potential for Skin Repair Applications.","authors":"Giuseppe Melilli, Patricia Rousselle, Mohamed Mehiri, Nathanael Guigo, Didier Pin, Nicolas Sbirrazzuoli","doi":"10.1021/acsabm.5c00010","DOIUrl":"10.1021/acsabm.5c00010","url":null,"abstract":"<p><p>Despite advances in wound treatment through tissue engineering, the rapid colonization of biomaterials by host cells remains a crucial step toward complete wound healing. Thanks to their excellent biocompatibility, biodegradability, low antigenicity and cost-effectiveness, cross-linked hydrogels have attracted much attention as a viable solution for wound treatment. In this work, we have developed an inovative cross-linking method for gelatin-based hydrogels inspired by the wound closure mechanism of the green algae <i>Caulerpa taxifolia</i>. Caulerpenyne (CYN), a metabolite extracted from the algae, was used as a latent cross-linking agent for gelatin. The covalent cross-linking process is triggered by an in situ and on-demand deacetylation of the enol acetate functionalities of CYN in oxytoxin 2 (OXY) containing 1,4-dialdehyde, which immediately reacts with the lysine residue in gelatin. The content of ε-amino groups in gelatin was monitored as a function of CYN concentration. Swelling and gel content were analyzed as a function of CYN concentration. Morphology, rheological and biological properties were evaluated by in vitro and in vivo tests. Cell adhesion and viability tests performed with OXY-cross-linked hydrogels and compared with non-cross-linked and genipin-cross-linked gelatin showed excellent performance. Their use in whole skin wounds in pigs showed that CYN-cross-linked hydrogels promoted complete skin regeneration without any cytotoxicity, making them extremely promising matrices in the field of regenerative medicine.</p>","PeriodicalId":2,"journal":{"name":"ACS Applied Bio Materials","volume":" ","pages":"2558-2568"},"PeriodicalIF":4.6,"publicationDate":"2025-03-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143381129","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Activating Thermoplastic Polyurethane Surfaces with Poly(ethylene glycol)-Based Recombinant Human α-Defensin 5 Monolayers for Antibiofilm Activity.","authors":"Xavier Rodríguez Rodríguez, Adrià López-Cano, Karla Mayolo-Deloisa, Oscar Q Pich, Paula Bierge, Nora Ventosa, Cristina García-de-la-Maria, José M Miró, Oriol Gasch, Jaume Veciana, Judith Guasch, Anna Arís, Elena Garcia-Fruitós, Imma Ratera","doi":"10.1021/acsabm.4c00732","DOIUrl":"10.1021/acsabm.4c00732","url":null,"abstract":"<p><p>Addressing multidrug-resistant microbial infections linked to implantable biomedical devices is an urgent need. In recent years, there has been an active exploration of different surface coatings to prevent and combat drug-resistant microbes. In this research, we present a facile chemical modification of thermoplastic polyurethane (TPU) surfaces with poly(ethylene glycol)-based recombinant human α-defensin 5 (HD5) protein with antimicrobial activity. TPU is one of the most relevant materials used for medical devices with good mechanical properties but also good chemical resistance, which makes it difficult to modify. The chemical modification of TPU surfaces is achieved via a three-step procedure based on (i) TPU activation using hexamethylene diisocyanate (HDI); (ii) interfacial reaction with poly(ethylene glycol) (PEG) derivatives; and finally, (iii) a facile click reaction between the PEG-maleimide terminated assembled monolayers on the TPU and the cysteine (-thiol) termination of the recently designed recombinant human α-defensin 5 (HD5) protein. The obtained PEG based HD5 assembled monolayers on TPU were characterized using a surface science multitechnique approach including scanning electron microscopy, atomic force microscopy, contact angle, and X-ray photoelectron spectroscopy. The modified TPU surfaces with the HD5 protein derivative exhibit broad-spectrum antibacterial properties reducing biofilm formation against <i>Pseudomonas aeruginosa</i> (Gram-negative), methicillin-resistant <i>Staphylococcus aureus</i> (MRSA) (Gram-positive) and methicillin-resistant <i>Staphylococcus epidermidis</i> (MRSE) (Gram-positive). These findings underscore the substantial potential of protein-modified TPU surfaces for applications in combating bacterial infections associated with implantable materials and devices.</p>","PeriodicalId":2,"journal":{"name":"ACS Applied Bio Materials","volume":" ","pages":"1900-1908"},"PeriodicalIF":4.6,"publicationDate":"2025-03-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11921020/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143466582","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}