ACS Applied Bio Materials最新文献

{"title":"Inhibition of Phosphoglycerate Kinase 1 (PGK1) Decreases Neointimal Hyperplasia after Patch Angioplasty.","authors":"Peng Sun, Hao Cui, Changwei Ren, Chenzhen Xu, Chuanjie Yue, Jing'an Li, Yongqiang Lai","doi":"10.1021/acsabm.5c00589","DOIUrl":"10.1021/acsabm.5c00589","url":null,"abstract":"<p><p>Neointimal hyperplasia is a known complication following aorta interventions. In this study, our hypothesis was that inhibiting phosphoglycerate kinase 1 (PGK1) could effectively reduce aortic neointimal hyperplasia in a rat model of abdominal aortic patch angioplasty. The role of the glycolytic pathway in patch angioplasty was analyzed by next-generation sequencing data, and the core role of PGK1 was found by differential gene analysis. The rats were allocated into two distinct groups: a control group that did not receive any supplementary treatment and a group treated with NG52, an inhibitor of PGK1, which was administered via a PLGA coating. Abdominal aortic patches were surgically implanted in the rats and subsequently harvested on the 14th day postimplantation for further analysis. Immunohistochemical analysis identified the presence of PGK1-positive cells within the neointima of the rat model subjected to abdominal aortic patch angioplasty. Importantly, the use of NG52 PLGA coating significantly decreased neointimal thickness (<i>p</i> < 0.0001). The mechanism of action of NG52 may involve the inhibition of TGFβ1 expression and the activation of the signaling pathway. Consequently, targeting the PGK1 pathway holds promise as a therapeutic strategy to mitigate aortic neointimal hyperplasia.</p>","PeriodicalId":2,"journal":{"name":"ACS Applied Bio Materials","volume":" ","pages":"5930-5937"},"PeriodicalIF":4.6,"publicationDate":"2025-07-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144315481","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Nanohybrid Fibers via Direct Nanoparticle Injection into the Spider's Silk Gland.","authors":"Anastasia Kryuchkova, Mariia Mikhailova, Pavel Zelenovskii, Igor Bdikin, Andrei Kholkin, Elena F Krivoshapkina, Pavel V Krivoshapkin","doi":"10.1021/acsabm.5c00680","DOIUrl":"10.1021/acsabm.5c00680","url":null,"abstract":"<p><p>Spider silk demonstrates an impressive balance of high strength and elasticity, which results from the hierarchical self-assembled structure of spider silk proteins during the fiber biosynthesis and spinning process. Enhancing the mechanical characteristics of spider silk fibers and imparting them with functional properties has garnered considerable attention. This challenge underscores the importance of developing strategies for modifying native spider silk. In this study, we introduce an approach to modify the structure and properties of spider silk fibers by injecting magnetite hydrosols directly into the spiders' silk glands. This results not only in the magnetic functionality of spider silk fibers but also in 82% increase in Young's compared to native spider silk, along with hardness of 1.30 MPa. To explore the nature of this phenomenon, we analyzed the difference in the topography of native <i>Holothele incei</i> spider silk and Fe₃O₄-hybrid spider silk, as well as their corresponding mechanical behavior at the nanoscale. Additionally, we studied the changes in structure, composition, and morphology caused by the inclusion of magnetic nanoparticles. Our findings demonstrate that the polar and hydrophobic interactions between Fe₃O₄ nanoparticles and the amino acid residues in spider silk could influence Young's modulus and hardness of the Fe₃O₄/spider silk hybrid fibers by promoting the protein conformation from an amorphous phase to β-sheets. This can only be achieved when nanomaterials are integrated into the structure within the fiber. The developed approach enables the fabrication of modified spider silk fibers, which can aid in the fundamental study of native spider silk and the development of technologies to fully replicate the properties of native silk in the future. Furthermore, lightweight, flexible, but strong materials are critical in soft robotic applications, where these nanohybrid fibers not only ensure gentle manipulation and reliability, but also their magnetic properties allow for responsive movement and control.</p>","PeriodicalId":2,"journal":{"name":"ACS Applied Bio Materials","volume":" ","pages":"6145-6158"},"PeriodicalIF":4.6,"publicationDate":"2025-07-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144315483","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Recent Trends in Hydrogel-Based Biosensor Technology for the Diagnosis of Neurodegenerative Diseases.","authors":"Seda Ayçiçek, Şule Coşkun Cevher, Selim Acar","doi":"10.1021/acsabm.5c00420","DOIUrl":"10.1021/acsabm.5c00420","url":null,"abstract":"<p><p>Early diagnosis of neurodegenerative diseases is essential for the development of appropriate treatment strategies. Current diagnostic methods have a number of limitations and challenges. Alternative biomaterials such as hydrogels are potential candidates that are attracting attention in the design process of biosensors for the diagnosis of neurodegenerative diseases. Three-dimensional networks of cross-linked hydrophilic polymers, known as hydrogels, have characteristics that are comparable to those of biological tissues. The integration of hydrogels into biosensor designs for the diagnosis of neurodegenerative diseases offers functionality and structural advantages. This review summarizes recent trends in hydrogel-based biosensors for the detection of neurodegenerative diseases, their design, applications, as well as a comprehensive overview of their advantages and disadvantages.</p>","PeriodicalId":2,"journal":{"name":"ACS Applied Bio Materials","volume":" ","pages":"5424-5444"},"PeriodicalIF":4.6,"publicationDate":"2025-07-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144367621","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Polycaprolactone-Polydopamine-Collagen Scaffold Loaded with Autologous Keratinocytes Accelerates Wound Healing.","authors":"Wenxuan Wang, Hao Zhang, Yuchen Dong, Rong Huang, Jiezhang Tang, Yige Han, Xueyong Li, Chiyu Jia, Xuekang Yang, Jing Li","doi":"10.1021/acsabm.5c00379","DOIUrl":"10.1021/acsabm.5c00379","url":null,"abstract":"<p><p>Extensive skin injuries often lead to chronic wound healing, scar formation, and elevated mortality rates. Existing treatment options, including autologous, allogeneic, and xenogeneic skin grafts, are constrained by donor scarcity, low graft survival rates, scarring, flap edema, and the inability to regenerate skin appendages. Consequently, the development of tissue-engineered skin substitutes comprising scaffolds, cells, and bioactive factors has emerged as a promising approach for repairing extensive skin defects. Herein, we optimized the electrospinning technique to construct a three-dimensional polycaprolactone (PCL) nanofiber scaffold with a thickness of 5 μm ± 0.7 μm and a porosity of 85%. Dopamine self-polymerization was employed to form a polydopamine (PDA) coating, which was subsequently combined with type I collagen (COL) to produce a polycaprolactone-polydopamine-collagen (PPC) scaffold. The PPC scaffold demonstrated significantly enhanced mechanical strength and hydrophilicity, along with excellent biocompatibility. The biocompatibility of the PPC nanofiber scaffold was markedly improved, effectively promoting the migration, adhesion, and proliferation of keratinocytes (KCs). Co-culturing autologous KCs with the PPC scaffold to construct an epidermal membrane graft significantly accelerated wound healing, resulting in a more complete neo-skin tissue structure and improved wound healing quality. Moreover, qPCR and Western blot analyses were employed to assess key protein and gene expression levels in the Wnt signaling pathway, revealing that our epidermal cell membrane could activate the Wnt signaling pathway, thereby promoting wound healing. This tissue-engineered epidermis offers a promising alternative to traditional skin grafting methods and may address the limitations associated with extensive skin injuries.</p>","PeriodicalId":2,"journal":{"name":"ACS Applied Bio Materials","volume":" ","pages":"5689-5698"},"PeriodicalIF":4.6,"publicationDate":"2025-07-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144537370","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"","authors":"Anju Sangwan,&nbsp;Nitish Kumar and Neetu Singh*,&nbsp;","doi":"","DOIUrl":"","url":null,"abstract":"","PeriodicalId":2,"journal":{"name":"ACS Applied Bio Materials","volume":"8 7","pages":"XXX-XXX XXX-XXX"},"PeriodicalIF":4.6,"publicationDate":"2025-07-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://pubs.acs.org/doi/pdf/10.1021/acsabm.5c00529","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144665333","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"","authors":"Zhao Wang,&nbsp;Khawar Ali Shahzad,&nbsp;Ding Li,&nbsp;Boyu Cai,&nbsp;Zilin Huang,&nbsp;Maoxiang Xu,&nbsp;Jiaojiao Li,&nbsp;Dong Chen and Fei Tan*,&nbsp;","doi":"","DOIUrl":"","url":null,"abstract":"","PeriodicalId":2,"journal":{"name":"ACS Applied Bio Materials","volume":"8 7","pages":"XXX-XXX XXX-XXX"},"PeriodicalIF":4.6,"publicationDate":"2025-07-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://pubs.acs.org/doi/pdf/10.1021/acsabm.5c00387","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144665341","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"","authors":"Wenxuan Wang,&nbsp;Hao Zhang,&nbsp;Yuchen Dong,&nbsp;Rong Huang,&nbsp;Jiezhang Tang,&nbsp;Yige Han,&nbsp;Xueyong Li,&nbsp;Chiyu Jia*,&nbsp;Xuekang Yang* and Jing Li*,&nbsp;","doi":"","DOIUrl":"","url":null,"abstract":"","PeriodicalId":2,"journal":{"name":"ACS Applied Bio Materials","volume":"8 7","pages":"XXX-XXX XXX-XXX"},"PeriodicalIF":4.6,"publicationDate":"2025-07-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://pubs.acs.org/doi/pdf/10.1021/acsabm.5c00379","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144665344","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"","authors":"M. Nuruzzaman Khan*,&nbsp;M. Tarik Arafat,&nbsp;Taslim Ur Rashid,&nbsp;Papia Haque and Mohammed Mizanur Rahman,&nbsp;","doi":"","DOIUrl":"","url":null,"abstract":"","PeriodicalId":2,"journal":{"name":"ACS Applied Bio Materials","volume":"8 7","pages":"XXX-XXX XXX-XXX"},"PeriodicalIF":4.6,"publicationDate":"2025-07-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://pubs.acs.org/doi/pdf/10.1021/acsabm.5c01078","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144665347","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"","authors":"Takuma Watanabe,&nbsp;Gerardo Martin Quindoza,&nbsp;Yuta Aida,&nbsp;Hayato Laurence Mizuno,&nbsp;Yasutaka Anraku and Toshiyuki Ikoma*,&nbsp;","doi":"","DOIUrl":"","url":null,"abstract":"","PeriodicalId":2,"journal":{"name":"ACS Applied Bio Materials","volume":"8 7","pages":"XXX-XXX XXX-XXX"},"PeriodicalIF":4.6,"publicationDate":"2025-07-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://pubs.acs.org/doi/pdf/10.1021/acsabm.5c00595","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144665392","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"","authors":"Manaswini Ravipati,&nbsp;Simran Moorjani,&nbsp;Divyasri Ramasamy and Sushmee Badhulika*,&nbsp;","doi":"","DOIUrl":"","url":null,"abstract":"","PeriodicalId":2,"journal":{"name":"ACS Applied Bio Materials","volume":"8 7","pages":"XXX-XXX XXX-XXX"},"PeriodicalIF":4.6,"publicationDate":"2025-07-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://pubs.acs.org/doi/pdf/10.1021/acsabm.5c00325","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144665399","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}