ACS Applied Bio Materials最新文献

{"title":"Titanium Dioxide (TiO₂) Incorporation into Poly(ether sulfone) (PES) Hollow Fiber Membranes (HFMs) Improves Biocompatibility and Separation Performance for Bioartificial Kidney (BAK) and Hemodialysis Applications.","authors":"Nidhi Pandey, Neelakshi Kar, Jayesh Bellare","doi":"10.1021/acsabm.4c01309","DOIUrl":"10.1021/acsabm.4c01309","url":null,"abstract":"<p><p>Hemodialysis and bioartificial kidney (BAK), which mimic both physical and biological functions, can significantly impact chronic kidney disease (CKD) patients. Here we report on Hollow fiber membranes (HFMs) with enhanced separation of uremic toxins along with enhanced hemocompatibility and biocompatibility that also promote the growth of kidney cells. The improvement arises from the addition of titanium dioxide (0.1, 0.5, 2%) into poly(ether sulfone) (PES) HFMs during fiber spinning, resulting in enhanced growth and proliferation of HEK293 cells, which were able to form spheroids as evidenced by the confocal images. MTT cell viability assay, cell proliferation assay by cell count, live dead assay by flow cytometry, and reactive oxygen species (ROS) study showed metabolically active viable cells, a higher number of cells, and a low ROS level in TiO₂ PES HFMs. The ultrafiltration coefficients of HFMs were high, with the highest (152.86 ± 5.01 mL/(m²·h·mmHg)) being for 2% TiO₂ PES (2TiP), showing the enhanced separation performance and better hemocompatibility (<5% hemolysis limit), and the lowest being for 2TiP (0.057%), with a low value of complement activation and lowest SC5b-9 marker level (10.71 ng/mL). HFMs also showed enhanced separation of toxins such as urea, creatinine, lysozyme, and indoxyl sulfate, proving their capability to remove water-soluble, middle molecular weight and protein-bound toxins. Therefore, the TiO₂-incorporated membranes developed here have promise for hemodialysis and BAK applications.</p>","PeriodicalId":2,"journal":{"name":"ACS Applied Bio Materials","volume":" ","pages":"1065-1076"},"PeriodicalIF":4.6,"publicationDate":"2025-02-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143021272","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Melatonin-Loaded Hydrogel Modulates Circadian Rhythms and Alleviates Oxidative Stress and Inflammation to Promote Wound Healing.","authors":"Shujie Wu, Shiwen Yang, Linlin Ou, Hongjian Zhang, Lu Wang, Bingyu Feng, Zeyu Bai, Weichang Li, Bin Cheng, Wei Seong Toh, Juan Xia","doi":"10.1021/acsabm.4c01752","DOIUrl":"10.1021/acsabm.4c01752","url":null,"abstract":"<p><p>Circadian rhythm disruption, commonly caused by factors such as jet lag and shift work, is increasingly recognized as a critical factor impairing wound healing. Although melatonin is known to regulate circadian rhythms and has potential in wound repair, its clinical application is limited by low bioavailability. To address these challenges, we developed an alginate-based dual-network hydrogel as a delivery system for melatonin, ensuring its stable and sustained release at the wound site. This approach enhances the efficacy of melatonin in modulating the wound healing process. We investigated the effects of circadian rhythm disruption on the wound microenvironment under the influence of the melatonin-loaded hydrogel with a focus on its biocompatibility, hemostatic properties, and antioxidant response functions. Additionally, we elucidated the mechanisms by which the melatonin-loaded hydrogel system promotes wound healing. Our findings provide insights into the relationship between circadian rhythm disruption and wound healing, offering a promising strategy for the management of chronic wounds associated with circadian rhythm disorders.</p>","PeriodicalId":2,"journal":{"name":"ACS Applied Bio Materials","volume":" ","pages":"1607-1620"},"PeriodicalIF":4.6,"publicationDate":"2025-02-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143031488","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Mesoporous Silica with Dual Stimuli-Microenvironment Responsiveness via the Pectin-Gated Strategy for Controlled Release of Rosmarinic Acid.","authors":"Lina Song, Wenxian Chen, Xinbo Lu, Ziqiang Liu, Qingqing Rao, Weiqiang Xiao, Xiaoli Zhan, Shengxiang Yang, Feng Gao, Qinghua Zhang","doi":"10.1021/acsabm.4c01730","DOIUrl":"10.1021/acsabm.4c01730","url":null,"abstract":"<p><p>Traditional drug-delivery methods are limited by low bioavailability and nonspecific drug distribution, resulting in poor therapeutic efficacy and potential risks of toxicity. Mesoporous silica nanoparticles (MSNs) have attracted wide attention as drug-delivery carriers due to their large specific surface area, adjustable pore size, good mechanical strength, good biocompatibility, and rich hydroxyl groups on their surface. In this paper, MSNs were synthesized by a template method, and the morphology and pore structure were regulated. The obtained particles possessed a narrow particle size distribution and good sphericity with a diameter of 878 nm, and their growth process was consistent with the La-Mer growth mechanism. Furthermore, MSNs were modified with amino acids and loaded with rosmarinic acid (RosA), accompanied by the \"outer packaging\" of pectin to obtain RosA@MSNs-Pec. RosA was added at 200, 150, and 100 mg within 72 h, with a drug loading of 193, 175.5, and 156 mg/g and an encapsulation rate of 19.3, 17.6, and 15.6, respectively. Interestingly, RosA@MSNs-Pec demonstrated the dual pH and pectinase response property, and its release curve conformed to the Higuchi model, indicating that its drug-controlled release was based on Fick's law.</p>","PeriodicalId":2,"journal":{"name":"ACS Applied Bio Materials","volume":" ","pages":"1583-1593"},"PeriodicalIF":4.6,"publicationDate":"2025-02-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143050958","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Preventing <i>Candida albicans</i> Contamination on Packaged Ti-6Al-4V Alloy Surfaces by Cold Atmospheric Plasma Treatment.","authors":"Isau Dantas Morais, Luiz Emanuel Campos Francelino, Vanesca G S Leite, Gabriel M Martins, Jussier de Oliveira Vitoriano, Francisco Marlon C Feijó, Caio S Santos, Moacir F de Oliveira, Clodomiro Alves Júnior, Carlos Eduardo Bezerra de Moura","doi":"10.1021/acsabm.4c01422","DOIUrl":"https://doi.org/10.1021/acsabm.4c01422","url":null,"abstract":"<p><p>Recent investigations have demonstrated that opportunistic fungi, such as <i>Candida albicans</i>, are associated with the contamination of implantable devices, biofilm formation, and consequent resistance to antimicrobial treatment. Preventing biofilm formation on implantable device surfaces represents a significant challenge in medicine and dentistry. This study aimed to evaluate the effects of cold atmospheric plasma (CAP) treatment on Ti-6Al-4V alloy surfaces, sterilized in an autoclave at 120 °C for 20 min in surgical-grade paper packaging, focusing on their potential to optimize surface physicochemical properties and reduce <i>C. albicans</i> colonization. X-ray photoelectron spectroscopy (XPS) revealed the formation of Ti-O-H peaks and the oxidation of titanium (Ti³⁺ to Ti⁴⁺) on CAP-treated surfaces. Sessile drop tests demonstrated a significant improvement in wettability, with a reduction in contact angle (68.94° vs 36.1°, <i>p</i> < 0.05). Microbiological assays showed a reduction in <i>C. albicans</i> colony-forming units (CFUs) (42,500 ± 8,838 vs 24,000 ± 7,920; <i>p</i> < 0.05) and a decrease in pseudohyphae formation (32.7 ± 9.7 vs 11.6 ± 1.8; <i>p</i> < 0.05). Scanning electron microscopy (SEM) further confirmed a reduction in yeast aggregates on treated surfaces incubated with fungal strains for 90 min. Data normality was assessed using the Shapiro-Wilk test, and statistical comparisons were performed with <i>t</i> tests at a significance level of <i>p</i> < 0.05. These findings suggest that CAP is a promising tool for enhancing surface wettability and reducing fungal contamination on Ti-6Al-4V implants sealed in surgical-grade paper, offering potential benefits for medical and dental applications.</p>","PeriodicalId":2,"journal":{"name":"ACS Applied Bio Materials","volume":" ","pages":""},"PeriodicalIF":4.6,"publicationDate":"2025-02-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143431878","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Investigation to Understand the Role of Phase Variation in Red Emitting Eu³⁺-Doped Calcium Magnesium Silicate Phosphor for In Vitro Bioimaging.","authors":"Navya Sara Kuriyan, Ayswaria Deepti, Baby Chakrapani P S, Sabeena Mannilthodi","doi":"10.1021/acsabm.4c01779","DOIUrl":"10.1021/acsabm.4c01779","url":null,"abstract":"<p><p>Eu³⁺-doped silicate phosphors are gaining significant attention for bioimaging and scaffold development due to their narrow red emission, high color purity, quantum yield (QY), and large Stokes shift. These phosphors offer several advantages over conventional imaging techniques, such as good selectivity and sensitivity, simpler operation, reduced data acquisition time, cost-effectiveness, and nondestructive imaging. The luminescence properties of these phosphors can be enhanced by modifying synthesis methods, annealing conditions, and hosts and introducing multiple dopants. This study explores a novel approach for improving luminescence by modifying the crystal structures of Eu³⁺ doped calcium magnesium silicate (CMS:Eu³⁺) phosphors for in vitro bioimaging and potential scaffold development. The synthesized diopside (CaMgSi₂O₆:<i>x</i>Eu³⁺; <i>x</i> = 10, 15, and 20 mol %), merwinite (Ca₃MgSi₂O₈:15 mol % Eu³⁺), and akermanite (Ca₂MgSi₂O₇:15 mol % Eu³⁺) phases of CMS:Eu³⁺ exhibit distinct coordination environments for Eu³⁺, leading to unique excitation wavelength tunability from ultraviolet (UV) to the visible region, high emission intensity, decay time, QY > 40%, and color purity >83%. A comparative analysis of their structural and photoluminescence properties reveals the impact of phase modifications on luminescence for in vitro bioimaging by optimizing the dopant concentration. The results indicate that CaMgSi₂O₆: 15 mol % Eu³⁺ is the most efficient phosphor for in vitro bioimaging, with the highest relative emission intensity in the red region, decay time ∼2 ms, QY ∼ 77%, and color purity ∼86%. The unique morphology of Ca₃MgSi₂O₈:15 mol %Eu³⁺ and Ca₂MgSi₂O₇:15 mol % Eu³⁺ also supports cell adhesion, suggesting their potential in scaffold development. In brief, the study highlights the potential of CMS:Eu³⁺ phosphors for in vitro bioimaging and scaffold development by modifying phases and dopant concentrations.</p>","PeriodicalId":2,"journal":{"name":"ACS Applied Bio Materials","volume":" ","pages":"1646-1663"},"PeriodicalIF":4.6,"publicationDate":"2025-02-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143254089","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Recent Advances of Carbon Dots: Synthesis, Plants Applications, Prospects, and Challenges.","authors":"Zhiwei Zhang, Caijuan Wu, Juan Hu, Chen Li, Yingliang Liu, Bingfu Lei, Mingtao Zheng","doi":"10.1021/acsabm.4c01785","DOIUrl":"10.1021/acsabm.4c01785","url":null,"abstract":"<p><p>Nanomaterials and nanotechnology have garnered significant attention in the realm of agricultural production. Carbon dots (CDs), as a class of nanomaterials, play a crucial role in the field of plant growth due to their excellent properties. This review aims to summarize recent achievements on CDs, focusing on their methods of preparation and applications in plants systems. The effects of CDs on seed germination, growth, photosynthesis, nutritional quality, and stress resistance were studied. It has been demonstrated that CDs can promote seed germination and growth, as well as improve photosynthetic efficiency, ultimately leading to increase plants yield. The nutritional quality of the plants treated with CDs was significantly improved. Specifically, the levels of essential mineral elements, vitamins, amino acids, and other constituents that are beneficial to human health increased notably. Additionally, CDs show positive effects on augmenting plant resistance against environmental stresses, such as drought conditions, heavy metal toxicity, and high salinity. Finally, the prospects and challenges of the application of CDs in plant systems are also discussed, which provide a scientific basis for the future application of CDs in agricultural production.</p>","PeriodicalId":2,"journal":{"name":"ACS Applied Bio Materials","volume":" ","pages":"935-961"},"PeriodicalIF":4.6,"publicationDate":"2025-02-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142981968","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Biocompatible EDOT-Pyrrole Conjugated Conductive Polymer Coating for Augmenting Cell Attachment, Activity, and Differentiation.","authors":"Nicolas Muzzio, Samantha Garcia, Luis Flores, Gary Newman, Amanda Gomez, Athena Santi, Mohamed Shahid Usen Nazreen, Eduardo Manuel Martinez-Cartagena, Delina Yirgaalem, Shrihari Sankarasubramanian, Gabriela Romero","doi":"10.1021/acsabm.4c01647","DOIUrl":"10.1021/acsabm.4c01647","url":null,"abstract":"<p><p>Developing scaffolds supporting functional cell attachment and tissue growth is critical in basic cell research, tissue engineering, and regenerative medicine approaches. Though poly(ethylene glycol) (PEG) and its derivatives are attractive for hydrogels and scaffold fabrication, they often require bioactive modifications due to their bioinert nature. In this work, biomimetic synthesized conductive polypyrrole-poly(3,4-ethylenedioxythiophene) copolymer doped with poly(styrenesulfonate) (PPy-PEDOT:PSS) was used as a biocompatible coating for poly(ethylene glycol) diacrylate (PEGDA) hydrogel to support neuronal and muscle cells' attachment, activity, and differentiation. The synthesized copolymer was characterized by Raman spectroscopy and dynamic light scattering. Its electrochemical properties were studied using galvanostatic charge-discharge (GCD) and voltammetry. PPy-PEDOT:PSS-coated hydrogels were characterized by Raman spectroscopy and atomic force microscopy, and protein adsorption was assessed using a quartz crystal microbalance with dissipation monitoring. Attachment and differentiation of the ND7/23 neuron hybrid cell line and C2C12 myoblasts were evaluated by cell cytoskeleton staining and quantification of morphological parameters. Viability was assessed by live/dead staining using flow cytometry. Cortex neural activity was studied by calcium ion influx that could be detected through the dynamic fluorescence changes of Fluo-4. The PPy-PEDOT:PSS coating supported cell attachment and differentiation and was nontoxic to cells. Primary neurons attached and remained responsive to electrical stimulation. Altogether, the biocompatible copolymer PPy-PEDOT:PSS is a simple yet effective alternative for hydrogel coating and presents great potential as an interface for nervous and other electrically excitable tissues.</p>","PeriodicalId":2,"journal":{"name":"ACS Applied Bio Materials","volume":" ","pages":"1330-1342"},"PeriodicalIF":4.6,"publicationDate":"2025-02-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143027427","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Sustainable Nonwoven Scaffolds Engineered with Recycled Carbon Fiber for Enhanced Biocompatibility and Cell Interaction: From Waste to Health.","authors":"Jonas Naumann, Kresten Singer, Siddharth Shukla, Alok Maurya, Stefan Schlichter, Imre Szenti, Akos Kukovecz, Amit Rawal, Mareike Zink","doi":"10.1021/acsabm.4c01475","DOIUrl":"https://doi.org/10.1021/acsabm.4c01475","url":null,"abstract":"<p><p>Carbon fibers, driven by ever-increasing demand, are contributing to a continuous rise in the generation of waste and byproducts destined for landfills or incineration. Recycling carbon fibers presents a promising strategy for reducing carbon emissions and conserving resources, thus contributing to more sustainable waste management practices. Discovering applications of recycled carbon fibers (rCFs) would inevitably accelerate the targeted integration of sustainable materials, fostering a circular economy. Herein, we have engineered rCF-based needlepunched nonwoven scaffolds and their blends with polypropylene (PP) fibers, providing the first example of investigating their interactions with human lung epithelial cells (Calu-3) and murine fibroblast cells (NIH/3T3). To promote the adsorption of extracellular matrix proteins such as laminin, these three-dimensional (3D) nonwoven scaffolds are designed and developed to feature tunable porous characteristics and wetting properties. Although cell adhesion and laminin adsorption are minimal on PP fibers, cells are preferentially organized on the rCFs. These nonwovens, composed exclusively of rCFs or their blends with PP fibers, exhibit no cytotoxic effects, with both cell types showing proliferation on the scaffolds and a progressive increase in cell numbers over time. Cell viability and apoptosis assays are also employed to comprehensively evaluate biocompatibility. Thus, our study proves rCF-based nonwoven scaffolds as potential candidates for artificial lung tissue scaffolds.</p>","PeriodicalId":2,"journal":{"name":"ACS Applied Bio Materials","volume":" ","pages":""},"PeriodicalIF":4.6,"publicationDate":"2025-02-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143439279","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Nanoparticle-Reinforced Hydrogel with a Well-Defined Pore Structure for Sustainable Drug Release and Effective Wound Healing.","authors":"Ziyi Zhang, Siyu Yang, Feixue Mi, Yicheng Yang, Qi Song, Yibo Gao, Changfeng Wu, Weijia Wen","doi":"10.1021/acsabm.4c01659","DOIUrl":"10.1021/acsabm.4c01659","url":null,"abstract":"<p><p>Impaired chronic wounds are a common complication of diabetes. Inhibited angiogenesis and dysfunctional inflammation render diabetic wound healing a critical challenge. Herein, a sustainable therapeutic composite hydrogel is presented for diabetic wound healing, consisting of a cocktail formulation of anti-inflammatory and local anesthetic nanoparticles incorporated into a composite hydrogel. The surface-modified drug nanoparticles are loaded into the biocompatible hydrogels and cross-linked with a gel precursor to enhance the structure. The sustainable delivery system achieves more than 90% drug release, with a total therapy duration tunable from 4 to 72 h. Through the long-lasting anti-inflammatory and analgesic effects of the composite hydrogel, diabetic wounds are swiftly transitioned into the proliferation phase, augmenting the survival and migration of keratinocytes and facilitating neovascularization and collagen alignment in diabetic wounds. These effects significantly improve the wound healing rate and skin regeneration process, achieving a healing rate that is 17 times that of untreated wounds. This study demonstrates that the hydrogel platform loaded with cocktail drug nanoparticles is promising for the rapid healing of diabetic wounds.</p>","PeriodicalId":2,"journal":{"name":"ACS Applied Bio Materials","volume":" ","pages":"1406-1417"},"PeriodicalIF":4.6,"publicationDate":"2025-02-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11836925/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143363243","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A Rhodamine-Based Ratiometric Fluorescent Sensor for Dual-Channel Visible and Near-Infrared Emission Detection of NAD(P)H in Living Cells and Fruit Fly Larvae.","authors":"Henry Lanquaye, Sushil K Dwivedi, Xinzhu Li, Peter Agyemang, Grace Rickauer, Dilka Liyana Arachchige, Crystal Wang, Joseph Peters, Ivy Zhen, Isabelle Knighton, Athar Ata, Thomas Werner, Haiying Liu","doi":"10.1021/acsabm.4c01912","DOIUrl":"10.1021/acsabm.4c01912","url":null,"abstract":"<p><p>The detection and dynamic monitoring of intracellular NAD(P)H concentrations are crucial for comprehending cellular metabolism, redox biology, and their roles in various physiological and pathological processes. To address this need, we introduce sensor <b>A</b>, a near-infrared ratiometric fluorescent sensor for real-time, quantitative imaging of NAD(P)H fluctuations in live cells. Sensor <b>A</b> combines a 3-quinolinium electron-deficient acceptor with a near-infrared rhodamine dye, offering high sensitivity and specificity for NAD(P)H with superior photophysical properties. In its unbound state, sensor <b>A</b> emits strongly at 650 nm and weakly at 465 nm upon 400 nm excitation. Upon binding to NAD(P)H, it shows a fluorescence increase at 465 nm and a decrease at 650 nm, enabling accurate ratiometric measurements. Sensor <b>A</b> also exhibits ratiometric upconversion fluorescence when excited at 800 or 810 nm, offering additional flexibility for different experimental setups. The sensor's response relies on the reduction of the 3-quinolinium acceptor by NAD(P)H, forming a 1,4-dihydroquinoline donor that enhances fluorescence at 465 nm and quenches the near-infrared emission at 650 nm through photoinduced electron transfer. This mechanism ensures high sensitivity and reliable quantification of NAD(P)H levels while minimizing interference from sensor concentration, excitation intensity, or environmental factors. Sensor <b>A</b> was validated in HeLa and MD-MB453 cells under various metabolic and pharmacological conditions, including glucose and maltose stimulation and treatments with chemotherapeutic agents. Co-localization with mitochondrial-specific dyes confirmed its mitochondrial targeting, enabling precise tracking of NAD(P)H fluctuations. <i>In vivo</i> imaging of <i>Drosophila</i> larvae under nutrient starvation or chemotherapeutic exposure revealed dose-dependent fluorescence responses, highlighting its potential for tracking NAD(P)H changes in live organisms. Sensor <b>A</b> represents a significant advancement in NAD(P)H imaging, providing a powerful tool for exploring cellular metabolism and redox biology in biomedical research.</p>","PeriodicalId":2,"journal":{"name":"ACS Applied Bio Materials","volume":" ","pages":"1707-1719"},"PeriodicalIF":4.6,"publicationDate":"2025-02-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143187520","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}