ACS Applied Bio Materials最新文献

{"title":"12-Hydroxy-Lauric Acid Tethered Self-Assembled Heterochiral Diphenylalanine-Based Mechanoresponsive and Proteolytically Stable Hydrogel: A Dual Player for Handling Cancer and Bacterial Challenges.","authors":"Rishabh Ahuja, Manju Singh, Anita Dutt Konar","doi":"10.1021/acsabm.4c01438","DOIUrl":"10.1021/acsabm.4c01438","url":null,"abstract":"<p><p>Deciphering the most promising strategy for the evolution of cancer patient management remains a multifaceted, challenging affair to date. Additionally, such approaches often lead to microbial infections as side effects, probably due to the compromised immunity of the patients undergoing such treatment. Distinctly, this work delineates a rational combinatorial strategy harnessing stereogenic harmony in the diphenylalanine fragment, tethering it to an amphiphile 12-hydroxy-lauric acid at the N-terminus (compounds <b>I</b>-<b>III</b>) such that a potential therapeutic could be extracted out from the series. Aligned to the goal, the cytotoxic properties and cell viability of the compounds were investigated using two distinct cell lines: MCF-7 (human breast cancer cell) and HEK 293 (human embryonic kidney). Our rigorous investigations revealed that compounds <b>I-III</b> exhibited substantial cytotoxic impact on the MCF7 cell line. But from a pool of three constructs, compound <b>III</b> (12-hydroxy-lauric acid -d-Phe-l-Phe-OH) showed better selectivity toward cancerous MCF7 over normal HEK 293 in comparison to others, backed by computational calculations. Henceforth, it was fished out from the series and used for its elaborate anticancer activities using cell reactive oxygen species generation, DNA fragmentation, and caspase-dependent gene expression employing extrinsic and intrinsic apoptotic factors as well as inflammatory biomarkers, namely, TNF-α and IL1-β. We anticipated that compound <b>III</b>, possessing mechanoresponsiveness and a nanofibrillar network, could be administered in patients with injections because of its shear-thinning properties. Moreover, the optimum partition coefficient of compound <b>III</b> might have allowed the scaffold to penetrate the cellular membranes and form a dilactate complex (compound <b>VI</b>) when exposed to the accumulated lactates/lactic acids, a common phenomenon observed within the hypoxic cancerous tumor cores, in accordance with the Warburg mechanism, thereby evading the cytotoxicity within normal cells. Besides, the supramolecular β-sheets of compound <b>III</b> manifest substantial antimicrobial efficacy against the common pathogens, two Gram-positive bacteria, <i>S. aureus</i> and <i>B. subtilis</i>, two Gram-negative bacteria, <i>E. coli</i> and <i>P. aeruginosa</i>, and a fungus, <i>C. albicans</i>, along with proteolytic stability and high mechanical strength at physiological pH. Overall, we speculate that the discovery of these multifunctional bioinspired materials holds future promise as preferential therapeutics for the remediation of immune-susceptible cancer patients, afflicted by microbial infections arising alone or as side effects of chemotherapeutic medications.</p>","PeriodicalId":2,"journal":{"name":"ACS Applied Bio Materials","volume":" ","pages":"1108-1125"},"PeriodicalIF":4.6,"publicationDate":"2025-02-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142995959","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Going for a Spin: Simultaneously Pulling and Spinning Microrods Speeds Transport through Collagen Matrices.","authors":"Lamar O Mair, Emily E Evans, Lester Barnsley, Aleksandar Nacev, Pavel Y Stepanov, Sahar Jafari, Benjamin Shapiro, Cindi L Dennis, Irving N Weinberg","doi":"10.1021/acsabm.4c01516","DOIUrl":"10.1021/acsabm.4c01516","url":null,"abstract":"<p><p>Magnetic drug targeting requires particles to move through the complex viscoelastic environments of tissues and biological fluids. However, these environments often inhibit particle motion, making it difficult for magnetically guided particles to reach their intended targets. Magnetic microrods are easy to grow and manipulate, but experience significant hindrance to transport in complex, tortuous, tissue-like environments. Simple magnetic force translation (\"pulling\" or \"pushing\") is often insufficient or inefficient for long-range transport of microrods through such environments. Designing microrods capable of rotating while being pulled with a magnetic force may enable rods to overcome hindrances to transport. We present microrods with orthogonally magnetized segments, actuated by simultaneous magnetic force and magnetic torque. By simultaneously pulling and rotating our rods we create smooth-surfaced magnetic drilling microrods (MDMRs) capable of enhanced motion through protein-dense biopolymers. We model magnetic force and torque on MDMRs, characterize MDMR dynamics during transport, and demonstrate enhanced MDMR transport through protein-dense matrices in vitro.</p>","PeriodicalId":2,"journal":{"name":"ACS Applied Bio Materials","volume":" ","pages":"1201-1209"},"PeriodicalIF":4.6,"publicationDate":"2025-02-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143121649","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Exploring the Unique Properties and Superior Schwann Cell Guiding Abilities of Spider Egg Sac Silk.","authors":"Karolina Peter, Sarah Stadlmayr, Aida Naghilou, Leon Ploszczanski, Manuel Hofmann, Christian Riekel, Jiliang Liu, Manfred Burghammer, Claudia Gusenbauer, Johannes Konnerth, Hannes C Schniepp, Harald Rennhofer, Gerhard Sinn, Christine Radtke, Helga C Lichtenegger","doi":"10.1021/acsabm.4c01587","DOIUrl":"10.1021/acsabm.4c01587","url":null,"abstract":"<p><p>Spider silk (SPSI) is a promising candidate for use as a filler material in nerve guidance conduits (NGCs), facilitating peripheral nerve regeneration by providing a scaffold for Schwann cells (SCs) and axonal growth. However, the specific properties of SPSI that contribute to its regenerative success remain unclear. In this study, the egg sac silk of <i>Trichonephila (T.) inaurata</i> is investigated, which contains two distinct fiber types: tubuliform (TU) and major ampullate (MA) silk. These fibers serve as models to derive material parameters governing SC migration on natural silk substrates, since they are produced by the same spider, yet exhibiting distinct composition and morphology. In this paper, detailed characterization of the fibers' material properties and <i>in vitro</i> evaluation of their SC-guiding performance were conducted. Live cell imaging revealed significantly enhanced SC mobility and directionality on TU silk compared to MA silk, which is remarkable, given the lack of studies on TU silk for nerve regeneration. Our results suggest that the distinct morphological and material properties of these fibers are critical to their nerve-guiding potential. These insights contribute to the optimization of NGC filler materials by identifying key parameters essential for effective nerve regeneration.</p>","PeriodicalId":2,"journal":{"name":"ACS Applied Bio Materials","volume":" ","pages":"1307-1319"},"PeriodicalIF":4.6,"publicationDate":"2025-02-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11836930/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142995967","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Sodium Carboxymethylcellulose/Polydopamine Biocellulose Coatings with Enhanced Wet Stability for Implantable Medical Devices.","authors":"Yehao Jiang, Zainab Ayaz, Long Xiang, Lili Zhou, Leila Mamizadeh, Yong Wang, Xie Dong, Nan Huang, Yongxiang Leng, Behnam Akhavan, Fengjuan Jing","doi":"10.1021/acsabm.4c01278","DOIUrl":"10.1021/acsabm.4c01278","url":null,"abstract":"<p><p>Sodium carboxymethylcellulose (CMC) is a biocompatible and biodegradable derivative of cellulose, making it a promising material for biomedical applications. However, its poor stability in aqueous environments has significantly limited its use in long-term biomedical devices. Here, we present for the first time a simple and controllable method to enhance the wet stability of CMC coatings by cross-linking of CMC and polydopamine (PDA) and self-polymerization of PDA for widespread applications in biomedical devices. A series of CMC/PDA coatings were fabricated on the initial PDA layers by using dip coating and subsequently heated at 200 °C. The performance of the CMC/PDA coatings and their chemical and structural stability in aqueous media have been systematically analyzed, and the mechanisms underpinning their robust performance have been revealed. FITR, X-ray photoelectron spectroscopy (XPS), and gel permeation chromatography (GPC) results showed that CMC/PDA coatings involved amidation and esterification reactions as well as self-polymerization of PDA. Degradation studies in phosphate-buffered saline (PBS) solution at 37 °C indicated degradation via ester and amide bond cleavage, with the stability of CMC/PDA coatings surpassing that of individual PDA and CMC coatings over a 30-day immersion period. The CMC/PDA coating with a CMC concentration of 15 mg/mL exhibited the highest adhesion strength in an aqueous environment, which was attributed to the high cross-linking of CMC and PDA, as well as the intrinsic stability of PDA. The CMC/PDA coatings demonstrated favorable viability, growth, and proliferation of endothelial cells. The stable and biocompatible biocellulose coatings can be easily applied from aqueous solutions onto almost any type of solid metal and ceramic material, providing a promising dimension for surface engineering of vascular scaffolds and tissue engineering constructs.</p>","PeriodicalId":2,"journal":{"name":"ACS Applied Bio Materials","volume":" ","pages":"1050-1064"},"PeriodicalIF":4.6,"publicationDate":"2025-02-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143121651","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Physicochemically Cross-linked Injectable Hydrogel: an Adhesive Skin Substitute for Burned Wound Therapy.","authors":"Mina Shahriari-Khalaji, Mamoona Sattar, Huidan Wei, Mastafa H Al-Musawi, Yahiya Ibrahim Yahiya, Sumyah Hasan Torki, Shengyuan Yang, Mohamadreza Tavakoli, Marjan Mirhaj","doi":"10.1021/acsabm.4c01592","DOIUrl":"10.1021/acsabm.4c01592","url":null,"abstract":"<p><p>Burns carry a large surface area, varying in shapes and depths, and an elevated risk of infection. Regardless of the underlying etiology, burns pose significant medical challenges and a high mortality rate. Given the limitations of current therapies, tissue-engineering-based treatments for burns are inevitable. Herein, we developed a natural physicochemically cross-linked adhesive injectable skin substitute (SS) comprising chitosan (Ch) and silk fibroin (SF), cross-linked with tannic acid (TA) through hydrogen bonding, and incorporated with fresh platelet-rich fibrin (FPRF). SF was also chimerically cross-linked with riboflavin (RF) under visible light to ensure desirable biodegradability rate and nontoxicity. Double cross-linked SS exhibited a semibilayer (SBSS) structure with smaller pores in the upper layer. In the CaCl₂-treated FPRF, the activated platelets augmented vascular endothelial growth factor (VEGF) and platelet-derived GF (PDGF) release. The resultant SBSS possessed optimal adhesion, hemocompatibility, and significant antibacterial and antioxidant activities (<i>P</i> ≤ 0.05). The rat liver injury model confirmed the rapid hemostatic effect of SBSS. Furthermore, the bottom layer of SBSS promoted L929 fibroblast growth, proliferation, and migration. SBSS-treated wounds showed lower inflammatory cells, earlier epithelialization, significant angiogenesis, and faster healing. The proposed SBSS could be an ideal remedy for burn wound therapy.</p>","PeriodicalId":2,"journal":{"name":"ACS Applied Bio Materials","volume":" ","pages":"1292-1306"},"PeriodicalIF":4.6,"publicationDate":"2025-02-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142996002","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Mucin-Based Dual Cross-Linkable IPN Hydrogel Bioink for 3D Bioprinting and Cartilage Tissue Engineering.","authors":"Sruthi C Sasikumar, Upashi Goswami, Ashok M Raichur","doi":"10.1021/acsabm.4c01505","DOIUrl":"10.1021/acsabm.4c01505","url":null,"abstract":"<p><p>The cartilage possesses limited regenerative capacity, necessitating advanced approaches for its repair. This study introduces a bioink designed for cartilage tissue engineering (TE) by incorporating ionically cross-linkable alginate into the photo-cross-linkable MuMA bioink, resulting in a double cross-linked interpenetrating network (IPN) hydrogel. Additionally, hyaluronic acid (HA), a natural component of cartilage and synovial fluid, was added to enhance the scaffold's properties. HA has been demonstrated to improve cartilage lubrication, regulate inflammation, promote cell proliferation, and support extracellular matrix (ECM) deposition and regeneration, making it valuable for cartilage TE. Comprehensive experiments were conducted to assess morphology, swelling, degradation, mechanical and rheological properties, printability, and biocompatibility. Results indicated that the double cross-linked scaffolds comprising MuMA, alginate, and HA exhibited compressive moduli comparable to native cartilage, unlike single cross-linked variants. The double cross-linking also influenced degradation, water uptake, and porosity, contributing to the scaffold durability and stability for chondrocyte support. Biocompatibility tests with C28/I2 cells demonstrated the cell-supportive and chondrogenic potential of the bioink. This study establishes mucin as a versatile material for specialized cartilage tissue engineering applications.</p>","PeriodicalId":2,"journal":{"name":"ACS Applied Bio Materials","volume":" ","pages":"1186-1200"},"PeriodicalIF":4.6,"publicationDate":"2025-02-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142995982","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Hyaluronic-Acid-Functionalized Tofacitinib Loaded Transethosomes for Targeted Drug Delivery in Rheumatoid Arthritis.","authors":"Pavani Chowdary, Eswara Rao Puppala, Chandra Lekha Putta, Jagapathi Rao Maddila, Vishnu Pulavarthy, V V S Rajendra Prasad, Aravind Kumar Rengan","doi":"10.1021/acsabm.4c01743","DOIUrl":"10.1021/acsabm.4c01743","url":null,"abstract":"<p><p>The Janus kinase inhibitor tofacitinib (TOF) is an FDA-approved drug for rheumatoid arthritis (RA) treatment, but its long-term oral use leads to significant systemic side effects. The present research aimed to conquer these challenges by formulating hyaluronic-acid-coated transethosomes (HA-TOF-TE), a novel system for targeted, topical delivery of TOF to reduce systemic toxicity and improve therapeutic efficacy. Transethosomes were synthesized via the cold sonication technique with HA functionalization enabling CD44 receptor-mediated targeting of inflamed synovial tissue. Optimized TOF-TE and HA-TOF-TE formulations showed particle sizes of 199.08 ± 4.2 and 151.5 ± 5.4 nm, zeta potentials of -27.1 ± 0.75 and -34.10 ± 0.89 mV, and entrapment efficiencies of 81.16 ± 0.84% and 79.19 ± 2.65%, respectively. The gels were assessed through <i>in vitro</i> drug release, <i>ex vivo</i> permeability, and <i>in vivo</i> effectiveness experiments using Freund's complete adjuvant (CFA) model. <i>Ex vivo</i> studies showed 2.02-fold and 1.61-fold increments in flux for TOF-TE and HA-TOF-TE, respectively, with superior skin retention for HA-TOF-TE. <i>In vivo</i> efficacy confirmed HA-TOF-TE's significant (<i>P</i> < 0.001) anti-inflammatory effect on arthritic rat paws, outperforming TOF-TE and FD gels. Cytokine analysis showed notable reductions in serum IL-1, IL-6, and PGE-2 levels after HA-TOF-TE treatment, closely approximating control values. Additionally, mRNA analysis demonstrated marked decreases in IL-6, CD44, and collagen II expression, indicating HA-TOF-TE's potential as an effective, targeted RA treatment, addressing the challenges of conventional TOF therapy and minimizing systemic side effects.</p>","PeriodicalId":2,"journal":{"name":"ACS Applied Bio Materials","volume":" ","pages":"1594-1606"},"PeriodicalIF":4.6,"publicationDate":"2025-02-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143057489","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Durable Bio-Based Hydrophobic Recrystallized Wax Coatings.","authors":"Santhra Krishnan P, Sriharitha Rowthu, Sreeram K Kalpathy","doi":"10.1021/acsabm.4c01672","DOIUrl":"10.1021/acsabm.4c01672","url":null,"abstract":"<p><p>Bio-waxes derived from natural species are beneficial for preparing non-wetting surfaces. Herein, the wetting properties of recrystallized wax coatings extracted from three naturally occurring superhydrophobic species-, Lotus leaves, Bauhinia leaves, and Periwinkle flowers, are reported as a function of recrystallization time, temperature, pH of water, and impact pressure. Lotus wax coatings showcased nanorods similar to that of Lotus leaves, while Periwinkle and Bauhinia waxes could not replicate micro-/nanofeatures from their respective natural species. Lotus wax coatings exhibited water contact angles (WCAs) of ∼150°, roll-off angles (RAs) of ∼8°, and self-cleaning properties. On the contrary, both Periwinkle and Bauhinia waxes showed WCAs of only ∼110°. Nevertheless, all coatings demonstrated remarkable temporal stability over 180 days, retaining their hydrophobicity. They also exhibit excellent thermal stability up to 100 °C and chemical stability for pH variations from 2.6 to 11.5. Furthermore, they withstand the impact of 3000 water droplets without losing their hydrophobicity. All three wax coatings showed very low moisture absorption coefficients in the order Periwinkle (5.5 × 10^-4 wt %/day) < Bauhinia (6.75 × 10^-4 wt %/day) < Lotus (1.075 × 10^-3 wt %/day), making them highly effective for moisture resistant applications such as food packaging, protective wood finishes, etc.</p>","PeriodicalId":2,"journal":{"name":"ACS Applied Bio Materials","volume":" ","pages":"1437-1450"},"PeriodicalIF":4.6,"publicationDate":"2025-02-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143062118","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Magnetic Cell Separation Based on Protein Nanoparticles Mediating the Interaction between Magnetic Particles and Target Cells.","authors":"Kei Nishida, Gaoyang Wang, Eiry Kobatake, Masayasu Mie","doi":"10.1021/acsabm.4c01450","DOIUrl":"10.1021/acsabm.4c01450","url":null,"abstract":"<p><p>Isolation of specific cells from biological samples is an important aspect of various biological research and diagnostic applications. Magnetic separation using magnetic particles (MPs) allows for easy and specific isolation of the target cells. However, depending on the target cell antigen, biological ligands, such as antibodies, must be modified or altered on MPs. Additionally, further biological evaluation of isolated cells requires the removal of MPs from cells by the enzymatic degradation of the biological ligands. In this study, we designed a magnetic cell separation system in which temperature-responsive protein nanoparticles mediated the interaction between target cells and MPs, achieving the easy changeability of biological ligands, removal of MPs by cooling, and effective cell isolation. The protein nanoparticles were thermally responsively formed from fusion proteins constituted of elastin-like polypeptide (ELP), poly(aspartic acid) [poly(d)], and proteins-of-interest such as NanoLuc luciferase (Nluc) fused with replication initiation protein (Rep) (ELP-poly(d)-Nluc-Rep) or biotin acceptor peptide (BAP) (ELP-poly(d)-Nluc-BAP). Rep exhibited enzymatic conjugation activity with an optional DNA aptamer to protein nanoparticles. The transmembrane glycoprotein mucin 1 (MUC1)-binding DNA aptamer was conjugated to Rep as a model aptamer. Bioluminescence signals emitted from the Nluc domains were used to analyze the binding abilities. BAP contributed to binding to streptavidin-modified MPs via a biotin-streptavidin interaction. The MUC1-conjugated protein nanoparticles bound to MUC1-positive human breast cancer MCF-7 cells via MUC1 aptamers and streptavidin-conjugated MPs via BAP, leading to magnetic cell separation. The ratio of isolated MCF-7 cells via magnetic separation was 71.3% for the MCF-7 suspension at 1000 cells/1 mL. The MPs bound on recovered MCF-7 cells were removed by cooling at 4 °C to induce the dissociation of protein nanoparticles. Magnetic cell separation systems that use protein nanoparticles are a promising technology for biological research and diagnostic applications.</p>","PeriodicalId":2,"journal":{"name":"ACS Applied Bio Materials","volume":" ","pages":"1126-1137"},"PeriodicalIF":4.6,"publicationDate":"2025-02-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143254092","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Design and Ex Vivo Evaluation of a PCLA Degradable Device To Improve Annulus Fibrosus Repair.","authors":"Chloé Falcoz, Mansoor Chaaban, Cédric Paniagua, Marion Fusellier, Jérôme Guicheux, Catherine Le Visage, Benjamin Nottelet, Xavier Garric, Coline Pinese","doi":"10.1021/acsabm.4c01415","DOIUrl":"10.1021/acsabm.4c01415","url":null,"abstract":"<p><p>With a prevalence of over 90% in people over 50, intervertebral disc degeneration (IVDD) is a major health concern. This weakening of the intervertebral discs can lead to herniation, where the nucleus pulpus (NP) leaks through the surrounding Annulus Fibrosus (AF). Considering the limited self-healing capacity of AF tissue, an implant is needed to restore its architecture and function. Here, we developed a biomimetic electrospun nanofibrous biodegradable scaffold that could be potentially used to repair AF defects. To that aim, we synthesized copolymers and blends of ε-caprolactone and lactide to create poly(ε-caprolactone-<i>co</i>-lactide) (PCLA) and PCL/PLA scaffolds with 10, 20, or 30% PLA. Properties of the initial nanofibrous scaffolds and the impact of gamma irradiation sterilization on the mechanical, thermal, and <i>in vitro</i> degradation properties are assessed and discussed with respect to the AF application. It was shown that ovine AF cells colonize the nanofibrous layers with increased metabolic activity over time. As an outcome of these studies, two copolymers were chosen to design a device composed of a 3D nanofibrous stacked scaffold associated with a degradable anchoring system to maintain the scaffold in an AF defect. The implantability of this device was tested in a cadaveric sheep lumbar IVD.</p>","PeriodicalId":2,"journal":{"name":"ACS Applied Bio Materials","volume":" ","pages":"1097-1107"},"PeriodicalIF":4.6,"publicationDate":"2025-02-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142976713","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}