ACS Applied Bio Materials最新文献_第7页

IF 4.6

ACS Applied Bio Materials Pub Date : 2025-07-21

Yujiao Tang, Xiaomin Zhao, Zhenlin Nie, Bangshun He* and Dawei Deng*,

引用次数: 0

IF 4.6

ACS Applied Bio Materials Pub Date : 2025-07-21

Yufeng Zhu, Glen McHale, Hernan Barrio-Zhang, Rui Han, Gary G. Wells, Hongzhong Liu, Rodrigo Ledesma-Aguilar, Waldemar Vollmer, Nicholas Jakubovics and Jinju Chen*,

引用次数: 0

IF 4.6

ACS Applied Bio Materials Pub Date : 2025-07-21

Cody S. Madsen, Ashley V. Makela, Chima V. Maduka, Emily M. Greeson, Anthony Tundo, Evran Ural, Satyajit Hari Kulkarni, Ahmed A. Zarea, Matti Kiupel, Maryam Sayadi and Christopher H. Contag*,

引用次数: 0

Guanosine-Based Supramolecular Particles for Enhanced Drug and Gene Delivery in Cell Culture. 鸟苷基超分子颗粒在细胞培养中增强药物和基因传递。

IF 4.6

ACS Applied Bio Materials Pub Date : 2025-07-21 Epub Date: 2025-06-10 DOI: 10.1021/acsabm.5c00201

Luis M Negrón, Edwin Vázquez-Rosa, Luxene Belfleur, Tanya L Díaz, Bismark Madera-Soto, Irving E Vega, José M Rivera

{"title":"Guanosine-Based Supramolecular Particles for Enhanced Drug and Gene Delivery in Cell Culture.","authors":"Luis M Negrón, Edwin Vázquez-Rosa, Luxene Belfleur, Tanya L Díaz, Bismark Madera-Soto, Irving E Vega, José M Rivera","doi":"10.1021/acsabm.5c00201","DOIUrl":"10.1021/acsabm.5c00201","url":null,"abstract":"Supramolecular hacky sacks (SHS) are a distinct class of self-assembled colloidal particles derived from guanosine (G) derivatives, engineered to support a wide range of cellular and therapeutic functions. In this study, we examine how variations in G-derivative composition influence SHS cellular uptake, intracellular trafficking, and functional efficacy. Confocal microscopy and flow cytometry reveal that uptake is highly dependent on particle composition, indicating selective engagement with specific cellular mechanisms. We show that SHS particles are biocompatible carriers capable of delivering both small molecules and genetic material: they successfully encapsulate and release doxorubicin with enhanced cytotoxic effects, and enable plasmid transfection with sustained expression of fluorescent proteins. These findings position SHS particles as a highly adaptable and effective supramolecular platform for drug and gene delivery. Their intrinsic biodegradability, ease of preparation, and tunable bioactivity highlight their strong potential for advancing biomedical applications.","PeriodicalId":2,"journal":{"name":"ACS Applied Bio Materials","volume":" ","pages":"5625-5633"},"PeriodicalIF":4.6,"publicationDate":"2025-07-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12284859/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144264746","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Engineered Endosymbionts that Modulate Primary Macrophage Function and Attenuate Tumor Growth by Shifting the Tumor Microenvironment. 通过改变肿瘤微环境调节原代巨噬细胞功能和减弱肿瘤生长的工程内共生物质。

IF 4.6

ACS Applied Bio Materials Pub Date : 2025-07-21 Epub Date: 2025-06-24 DOI: 10.1021/acsabm.5c00590

Cody S Madsen, Ashley V Makela, Chima V Maduka, Emily M Greeson, Anthony Tundo, Evran Ural, Satyajit Hari Kulkarni, Ahmed A Zarea, Matti Kiupel, Maryam Sayadi, Christopher H Contag

{"title":"Engineered Endosymbionts that Modulate Primary Macrophage Function and Attenuate Tumor Growth by Shifting the Tumor Microenvironment.","authors":"Cody S Madsen, Ashley V Makela, Chima V Maduka, Emily M Greeson, Anthony Tundo, Evran Ural, Satyajit Hari Kulkarni, Ahmed A Zarea, Matti Kiupel, Maryam Sayadi, Christopher H Contag","doi":"10.1021/acsabm.5c00590","DOIUrl":"10.1021/acsabm.5c00590","url":null,"abstract":"Modulating gene expression in macrophages can be used to improve tissue regeneration and redirect tumor microenvironments (TMEs) toward positive therapeutic outcomes. We have developed Bacillus subtilis as an engineered endosymbiont (EES) capable of residing inside the eukaryotic host cell cytoplasm and controlling the fate of macrophages. Secretion of mammalian transcription factors (TFs) from B. subtilis that expresses listeriolysin O (LLO; allowing the EES to escape destruction by the macrophage) modulated expression of surface markers, cytokines, and chemokines, indicating functional changes in a macrophage/monocyte cell line. The engineered B. subtilis LLO TF strains were evaluated in murine bone marrow-derived macrophages (BMDMs) by flow cytometry, chemokine/cytokine profiling, metabolic assays, and RNA-Seq delivery of TFs by the EES shifted BMDM gene expression, production of cytokine and chemokines, and metabolic patterns, indicating that the TF strains could guide primary macrophage function. Thereafter, the ability of the TF strains to alter the TME was characterized in vivo in an orthotopic murine model of triple-negative breast cancer to assess therapeutic effects. The TF strains altered the TME by shifting immune cell composition and attenuating tumor growth. Additionally, multiple doses of the TF strains were well-tolerated by the mice. The use of B. subtilis LLO TF strains as EES showed promise as a unique cancer immunotherapy by directing the immune function intracellularly. The uses of EES could be expanded to modulate other mammalian cells over a range of biomedical applications.","PeriodicalId":2,"journal":{"name":"ACS Applied Bio Materials","volume":" ","pages":"5938-5958"},"PeriodicalIF":4.6,"publicationDate":"2025-07-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12284892/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144473267","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

BODIPY-Loaded ZIF-8 Nanomaterials for Enhanced Photodynamic Inactivation of Staphylococcus aureus. 负载bodipy的ZIF-8纳米材料增强金黄色葡萄球菌光动力失活。

IF 4.6

ACS Applied Bio Materials Pub Date : 2025-07-21 Epub Date: 2025-06-09 DOI: 10.1021/acsabm.5c00787

María E Pérez, Javier E Durantini, María E Milanesio, Edgardo N Durantini

{"title":"BODIPY-Loaded ZIF-8 Nanomaterials for Enhanced Photodynamic Inactivation of Staphylococcus aureus.","authors":"María E Pérez, Javier E Durantini, María E Milanesio, Edgardo N Durantini","doi":"10.1021/acsabm.5c00787","DOIUrl":"10.1021/acsabm.5c00787","url":null,"abstract":"The rise of antibiotic-resistant pathogens has heightened the demand for innovative treatments, promoting advanced photodynamic materials for targeted microbial inactivation. Here, we report the synthesis of a series of photosensitizer nanomaterials consisting of BODIPY (BDP) dyes confined within ZIF-8, prepared via a straightforward one-pot nanoprecipitation method. The morphology of these materials was analyzed by SEM and TEM, and spectroscopic characterization confirmed the successful incorporation of BDP dyes within the porous ZIF-8 framework. Encapsulation significantly improved the ability of BDPs to generate reactive oxygen species (ROS) in aqueous media, with the brominated derivative, Br2BDP@ZIF-8, exhibiting particularly high production of singlet molecular and superoxide anion radicals. The photodynamic activity of Br2BDP@ZIF-8 was further demonstrated by its efficient degradation of tryptophan through a type II photoprocess. In addition, encapsulating BDPs within the ZIF-8 framework significantly enhanced their photostability compared with the free dyes. Antimicrobial assays revealed the strong potential of these nanomaterials for photoinactivation of Staphylococcus aureus. Under green light irradiation, Br2BDP@ZIF-8 achieved a 99.999% reduction in the bacterial population in liquid suspensions after exposure to a light fluence of 5 J/cm2. Fluorescence images of S. aureus showed the characteristic green fluorescence of BODIPY, indicating an effective interaction with BDP@ZIF-8. Furthermore, effective eradication of S. aureus contamination (>97%) was found on surfaces irradiated with only 0.9 J/cm2. These findings highlight the potential of BDP-loaded ZIF-8 nanomaterials as robust and efficient photosensitizing agents for bacterial elimination, offering promising applications in the photokilling of bacterial cells and surface decontamination.","PeriodicalId":2,"journal":{"name":"ACS Applied Bio Materials","volume":" ","pages":"6316-6325"},"PeriodicalIF":4.6,"publicationDate":"2025-07-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144245185","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Multicolor Microscopic In Vivo Imaging of the Formation and Disappearance of Lipid Droplets Using BODIPY-Based Red and Deep-Red Fluorescent Probes. 使用基于bodipy的红色和深红色荧光探针的脂滴形成和消失的多色显微体内成像。

IF 4.6

ACS Applied Bio Materials Pub Date : 2025-07-21 Epub Date: 2025-06-12 DOI: 10.1021/acsabm.5c00698

Mayuko Shimanuki, Risa Uda, Shuichi Shiozaki, Toshitada Yoshihara

{"title":"Multicolor Microscopic In Vivo Imaging of the Formation and Disappearance of Lipid Droplets Using BODIPY-Based Red and Deep-Red Fluorescent Probes.","authors":"Mayuko Shimanuki, Risa Uda, Shuichi Shiozaki, Toshitada Yoshihara","doi":"10.1021/acsabm.5c00698","DOIUrl":"10.1021/acsabm.5c00698","url":null,"abstract":"Nonalcoholic fatty liver disease (NAFLD) is the most common chronic liver disease worldwide. As the number and size of lipid droplets (LDs) generally increase in hepatocytes during the early stages of NAFLD, visualization of LDs in living samples is of great importance for properly diagnosing and treating NAFLD. Here, red and deep-red fluorescent probes (BPMP-2Thia and BPMP-4Thia) based on boron-dipyrromethene (4,4-difluoro-4-bora-3a,4a-diaza-s-indacene, BODIPY) are developed for the visualization of LDs in the liver of normal and NAFLD model mice as well as cultured cells. BPMP-2Thia and BPMP-4Thia exhibit bright emission in low-polarity solvents, which simulate the microenvironment of LDs, and they exhibit high selectivity for LDs, costaining capability, good biocompatibility, and high photostability in living cells and mice. Multicolor confocal microscopy combined with intravenous administration of an LD-specific probe, vascular endothelial marker, and nucleus-specific probe in mice enables the quantitative visualization of LD growth, including visualization of the disordered sinusoidal network and flattened hepatocyte nuclei. Furthermore, this simultaneous imaging method allows evaluation of the reduction in the number and size of LDs in the hepatic tissues of fatty liver model mice upon shifting from a high-fat to normal diet.","PeriodicalId":2,"journal":{"name":"ACS Applied Bio Materials","volume":" ","pages":"6193-6202"},"PeriodicalIF":4.6,"publicationDate":"2025-07-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144281652","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

α-Fe Nanoparticles and Multiwalled Carbon Nanotubes Composite with Improved Photothermal Conversion Efficiency for Tumor Therapy. α-铁纳米粒子与多壁碳纳米管复合材料光热转换效率提高的肿瘤治疗

IF 4.6

ACS Applied Bio Materials Pub Date : 2025-07-21 Epub Date: 2025-06-12 DOI: 10.1021/acsabm.5c00772

Ihtisham Ahmad Butt, Peinan Yin, Naihan Chen, Wei Zhang

引用次数: 0

Exploring Lipid Droplets for Enhancing Cytoskeletal Stability and Repair Capacity via Carbonized Polymer Dot-Assisted Fluorescence Imaging. 通过碳化聚合物点辅助荧光成像探索脂滴增强细胞骨架稳定性和修复能力。

IF 4.6

ACS Applied Bio Materials Pub Date : 2025-07-21 Epub Date: 2025-06-16 DOI: 10.1021/acsabm.5c00834

Zitong Yu, Shuping Xu

引用次数: 0

Structural Impact of Anthracene-Appended Mn-MOF on Human Serum Albumin and Its Cellular Implications. 蒽附Mn-MOF对人血清白蛋白的结构影响及其细胞意义。

IF 4.6

ACS Applied Bio Materials Pub Date : 2025-07-21 Epub Date: 2025-06-20 DOI: 10.1021/acsabm.5c00887

Shanmugavel Chinnathambi, Mahima Kumar, Basudeb Dutta, Karthikeyan Subramani, Saravanan Kandasamy, Sebastian Kmiecik, Thangavel Vaijayanthi, Ganesh N Pandian

{"title":"Structural Impact of Anthracene-Appended Mn-MOF on Human Serum Albumin and Its Cellular Implications.","authors":"Shanmugavel Chinnathambi, Mahima Kumar, Basudeb Dutta, Karthikeyan Subramani, Saravanan Kandasamy, Sebastian Kmiecik, Thangavel Vaijayanthi, Ganesh N Pandian","doi":"10.1021/acsabm.5c00887","DOIUrl":"10.1021/acsabm.5c00887","url":null,"abstract":"Metal-organic frameworks (MOFs) are gaining attention as multifunctional nanomaterials for biomedical applications due to their porosity, tunable structure, and potential for molecular-level imaging. In this study, we synthesized green-emitting, water-dispersible manganese-based MOF (Mn-MOF) nanoparticles for live-cell imaging and investigated their interactions with human serum albumin (HSA). Spectroscopic analyses revealed high-affinity binding, with fluorescence quenching constants in the range of 1013. A red shift in emission and circular dichroism data confirmed that HSA retained its native conformation, underscoring the structural compatibility of Mn-MOFs. Biocompatibility was assessed using HeLa, A549, and chondrocyte cell lines. Cytotoxicity assays showed high cell viability at moderate concentrations and early time points. Nanoparticle size (∼18 nm by DLS; <10 nm by TEM) likely facilitated cellular uptake while minimizing toxicity. Confocal microscopy and flow cytometry revealed efficient internalization via multiple endocytic pathways, with perinuclear localization and no significant morphological changes. However, higher concentrations decreased cell adhesion and viability, indicating a dose-dependent toxicity threshold. These results demonstrate that Mn-MOF nanoparticles maintain protein integrity and exhibit low cytotoxicity, supporting their potential as safe, effective platforms for live-cell imaging and targeted delivery in nanomedicine.","PeriodicalId":2,"journal":{"name":"ACS Applied Bio Materials","volume":" ","pages":"6397-6414"},"PeriodicalIF":4.6,"publicationDate":"2025-07-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144332036","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0