ACS Applied Bio Materials最新文献

{"title":"Additive Effects of Natural Plant Extracts/Essential Oils and Probiotics as an Antipathogenic Topical Skin Patch Solution for Acne and Eczema.","authors":"Ling Xin Yong, Wenrui Li, Patricia L Conway, Say Chye Joachim Loo","doi":"10.1021/acsabm.4c01742","DOIUrl":"10.1021/acsabm.4c01742","url":null,"abstract":"<p><p>This work leverages the additive antipathogenic effects of natural extracts/essential oils (EOs) and probiotics for the treatment of acne vulgaris associated with <i>Cutibacterium acnes</i> (<i>C. acnes</i>) and eczema complicated by secondary infections with <i>Staphylococcus aureus</i> (<i>S. aureus</i>). Six probiotic strains and various extracts/EOs were evaluated in a large screening to evaluate their potential against both pathogens. <i>Lacticaseibacillus paracasei</i> PCB003 was able to inhibit the growth of both pathogens. For extracts/EOs, Oregano EO had the best antipathogenic effects on both pathogens and did not show any adverse impact on the growth of probiotics, making it suitable for simultaneous use. Using <i>Lactiplantibacillus plantarum</i> PCB011 as a probiotic model, five material formulations were assessed for their suitability to protect probiotic cells within freeze-dried topical patches. Alginate and trehalose (ALG+TRE) and thermoplastic starch (TPS) had the highest probiotic survivability, with ALG+TRE chosen as the final patch material as it was more robust. PCB003 and PCB011 were individually incorporated into the ALG+TRE freeze-dried matrix to form a 6 mm patch; both ALG+TRE (PCB003) and ALG+TRE (PCB011) patches, when used individually, successfully inhibited <i>C. acnes</i> growth by 4.7 and 6.0 mm, respectively, surpassing the performance of commercially available acne patches. The additive effect with 30% Oregano EO further improved pathogen inhibition. For <i>S. aureus</i>, the incorporation of 30% Oregano EO to ALG+TRE (PCB003) increased the size of the inhibition zone more than 10-fold. For <i>C. acnes</i>, the ALG+TRE (PCB003) patch with 30% Oregano EO demonstrated an inhibition zone of 16.3 mm, and the ALG+TRE (PCB011) patch with 30% Oregano EO achieved a 14.3 mm inhibition zone. Genomic analysis confirmed that PCB003 and PCB011 lack antimicrobial resistance determinants, ensuring safety. This study successfully combined probiotics and natural agents to create effective dermatological antipathogenic patches.</p>","PeriodicalId":2,"journal":{"name":"ACS Applied Bio Materials","volume":" ","pages":"1571-1582"},"PeriodicalIF":4.6,"publicationDate":"2025-02-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142995964","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Photothermal-Photocatalytic Ternary Heterostructure for Solar Light-Driven Highly Efficient Degradation of Antimicrobial Agents and Inactivation of Superbugs.","authors":"Kaelin Gates, Shivangee Rai, Olorunsola Praise Kolawole, Sanchita Kundu, Avijit Pramanik, Shruti Singh, Prabhat Bandari, Vishita Pandey, Deja Morehead, Rohan Alamgir, Zoe Edorodion, Tandabany Dinadayalane, Paresh Chandra Ray","doi":"10.1021/acsabm.4c01948","DOIUrl":"10.1021/acsabm.4c01948","url":null,"abstract":"<p><p>A significant proportion of antimicrobial agents, such as different antibiotics discharged into the environment via human and animal waste, poses significant problems for ecological balance and human health. Moreover, widespread overuse and misuse of antibiotics have led to antibiotic-resistant bacteria (superbugs), which is one of the biggest global health problems in the 21st century. Since the utilization of solar energy, which is an abundant and natural resource for the photocatalytic system, we report the design of a photothermal-photocatalytic dual-functional light absorber-based ternary heterostructure using plasmonic gold nanoparticle (AuNP)-anchored WO₃ nanoplatelet (WO₃ NPL)-decorated reduced graphene oxide (r-GO) (AuNP/WO₃ NPL/r-GO), which exhibits strong absorption between 400 and 900 nm regions and has the capability for the sunlight-driven 100% degradation of doxycycline antibiotics. Herein, we show that due to the excellent photothermal performance of AuNP and r-GO in the heterostructure, the local temperature increased under 785 NIR nm light irradiation, which boosted the photocatalytic degradation reaction kinetics for doxycycline antibiotics via enhancing the transfer of \"hot carriers\" and the formation of reactive oxygen species (ROS). Furthermore, experimental data indicate that by integrating photothermal-photocatalytic materials, sunlight can be used for 100% doxycycline antibiotic degradation after 80 min of light irradiation. Moreover, we demonstrate that the ternary heterostructure can be used for sunlight-based 100% inactivation of carbapenem-resistant Enterobacteriaceae <i>Escherichia coli</i> (CRE <i>E. coli</i>) and methicillin-resistant <i>Staphylococcus aureus</i> (MRSA) superbugs by just exposing them to light for 60 min. This study sheds light on the construction of photothermally assisted photocatalytic ternary heterostructures for high-efficiency sunlight-driven degradation of antibiotics and superbugs.</p>","PeriodicalId":2,"journal":{"name":"ACS Applied Bio Materials","volume":" ","pages":"1732-1744"},"PeriodicalIF":4.6,"publicationDate":"2025-02-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143254095","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"InP-Based Quantum Dots as Photosensitizers in Photodynamic Antimicrobial Materials.","authors":"Lihan Chen, Chenyu Jiang, Frank Scholle, Alissa E Meo, Jun Ohata, Christopher B Gorman, Reza A Ghiladi","doi":"10.1021/acsabm.4c01467","DOIUrl":"10.1021/acsabm.4c01467","url":null,"abstract":"<p><p>Ligand-functionalized InP-based quantum dots (QDs) have been developed as an innovative class of nontoxic photosensitizer suitable for antimicrobial applications, aimed at reducing or preventing pathogen transmission from one host to another via high contact surfaces. A hot injection method followed by functionalization via ligand exchange with 9-anthracene carboxylic acid (ACA) yielded the desired core/shell InP/ZnSe/ZnS QDs. Transmission electron microscopy (TEM) revealed these QDs to be uniform in size (∼3.2 nm), with light absorption across the entire visible spectrum (λ_max ∼550 nm). Under light excitation at 550 nm, the generation of singlet oxygen (¹O₂) was evidenced by its characteristic phosphorescence signal at 1278 nm, indicating successful energy transfer from the QDs to surface-anchored ACA ligands, in accordance with a type II mechanism for a photodynamically generated singlet oxygen. The InP/ZnSe/ZnS core/shell QDs were applied to cellulose via dip coating, and the resultant QDs-loaded material was assessed for antimicrobial photodynamic inactivation (aPDI) of both Gram-positive [methicillin-resistant <i>Staphylococcus aureus</i> (MRSA; ATCC-44), vancomycin-resistant <i>Enterococcus faecium</i> (VRE; ATCC-2320)] and Gram-negative [multidrug-resistant <i>Acinetobacter baumannii</i> (MDRAB; ATCC-1605), NDM-1 positive <i>Klebsiella pneumoniae</i> (KP; ATCC-2146)] bacteria under illumination (400-700 nm; 85 mW/cm²; 90 min). The highest inactivation was observed for MRSA, achieving at least 99.999% inactivation (5 log units). Antiviral photodynamic inactivation on human coronavirus 229E (HCoV-229E) and feline calicivirus (FCV) demonstrated complete viral inactivation (to the detection limit). Cytotoxicity studies showed that the QDs are nontoxic to mammalian cells in the dark. Together, these results confirm the promising potential of ligand-functionalized InP-based QDs to be employed as nontoxic photosensitizers as materials in self-sterilizing surfaces.</p>","PeriodicalId":2,"journal":{"name":"ACS Applied Bio Materials","volume":" ","pages":"1138-1147"},"PeriodicalIF":4.6,"publicationDate":"2025-02-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142995979","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Cold-Spray Deposition of Antibacterial Molybdenum Coatings on Poly(dimethylsiloxane).","authors":"Tzu-Ying Liao, Andrew Boden, Peter C King, Helmut Thissen, Russell J Crawford, Elena P Ivanova, Peter Kingshott","doi":"10.1021/acsabm.4c01380","DOIUrl":"10.1021/acsabm.4c01380","url":null,"abstract":"<p><p>Despite their widespread utilization in biomedical applications, these synthetic materials can be susceptible to microbial contamination, potentially compromising their functionality and increasing the risk of infection in patients. In this study, molybdenum (Mo), an essential metal in biological systems, was investigated as a Mo-based cold-sprayed coating on poly(dimethylsiloxane) (PDMS) for its potential use as biocompatible and antimicrobial surfaces for biomedical applications. Various cold-spray parameters were employed in the fabrication of Mo-embedded PDMS surfaces to alter the surface structure of the substrate, Mo loading density, and embedding layer thickness. Specifically, relatively low nozzle scanning speeds were used to develop high-density Mo-embedded PDMS surfaces. A comprehensive analysis was conducted to investigate how cold-spray processing parameters affect the surface topography, wettability, and chemical properties. The ability of the Mo-embedded PDMS to inhibit the colonization of <i>Staphylococcus aureus</i>, <i>Staphylococcus epidermidis</i>, <i>Escherichia coli</i>, and <i>Pseudomonas aeruginosa</i> bacterial species was demonstrated by both live/dead staining and disk diffusion methods. Surfaces with higher Mo loading densities significantly reduced the level of bacterial attachment and enhanced the bactericidal activity upon contact. Also, the level of Mo ion release over a 14-day period was measured and correlated to the properties of the substrate surface. Furthermore, attachment, viability, and proliferation of osteoblast-like MG63 cells were assessed to investigate the effect of Mo ion release on the biocompatibility of fabricated coatings. A notable decrease in cell viability and delayed growth of MG63 cells became evident after 7 days of incubation with the highly Mo-loaded samples. While this study enhanced our understanding regarding the engineering of composite materials for combatting microbial infections, the findings also suggest that the release of Mo ions may detrimentally affect osteoblast survival, potentially compromising the long-term functionality of orthopedic implants produced using this technique.</p>","PeriodicalId":2,"journal":{"name":"ACS Applied Bio Materials","volume":" ","pages":"1167-1185"},"PeriodicalIF":4.6,"publicationDate":"2025-02-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143027428","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Eco-Friendly Plant Polysaccharide-Based Sustainable Coating for Extension of Shelf Life and Removal of Pesticides in Horticulture Produce.","authors":"Dravin Pratap Singh, Unnikrishnan Babukuttan Sheela, Gopinath Packirisamy","doi":"10.1021/acsabm.4c01734","DOIUrl":"10.1021/acsabm.4c01734","url":null,"abstract":"<p><p>Excessive use of chemical pesticides in farming has raised great concerns about food safety because of pesticide contamination in fruits and vegetables. Pesticides like chlorpyrifos have been linked to liver and kidney damage, respiratory problems, neurological disorders, developmental delays, endocrine disruption, and gut microbiota alterations. Moreover, rapid spoilage of perishable agricultural produce like fruits and vegetables contributes to huge postharvest losses every year and worsens food security. This study addresses these two concerns by developing an eco-friendly coating made of guar gum, vinegar, and tamarind pulp. The coating's antibacterial properties were tested against <i>Escherichia coli</i> and <i>Staphylococcus aureus</i>, while its antioxidant capacity was measured using DPPH assay. The effectiveness of the coating in reducing pesticide residues was analyzed through high-performance liquid chromatography (HPLC). The results showed antibacterial activity and high antioxidant capacity, with free radical scavenging activity of 61.33% for vinegar, 47.81% for tamarind, and 49.9% for the composite. HPLC analysis showed that the coating reduced chlorpyrifos residues on treated produce compared to the control. Moreover, the coating extended the shelf life of perishable fruits and vegetables by reducing moisture loss and microbial growth. These findings suggest that the tamarind-vinegar-guar gum composite is a sustainable solution to pesticide contamination and postharvest losses and a significant contribution to food safety and security.</p>","PeriodicalId":2,"journal":{"name":"ACS Applied Bio Materials","volume":" ","pages":"1559-1570"},"PeriodicalIF":4.6,"publicationDate":"2025-02-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143187534","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Excision of HIV-1 Provirus in Human Primary Cells with Nanocapsuled TALEN Proteins.","authors":"Ming Zhao, Jing Wen, Irvin S Y Chen, Jia Liu, Yunfeng Lu","doi":"10.1021/acsabm.4c01544","DOIUrl":"10.1021/acsabm.4c01544","url":null,"abstract":"<p><p>Despite the tremendous success of combination antiretroviral therapy (ART) to treat human immunodeficiency virus (HIV) infection, the durability and persistence of latent reservoirs of HIV-infected cells in HIV-infected patients remain obstacles to achieving HIV cure. While technically challenging, the most direct means to eradicate latent reservoirs is to destroy the HIV provirus, thus ensuring that HIV virions are not produced while preserving resident cells. Transcription activator-like effector nucleases (TALEN)─a genome editing method with high DNA targeting efficiency─have been investigated as a potential gene therapy by disrupting the HIV-1 coreceptor CCR5 genes in HIV target cells or HIV proviral DNA in infected cells. However, the transduction and editing efficiencies are low in primary cells and vary by cell type. Using a nanotechnology platform, which we term nanocapsules, the TALEN protein can be effectively delivered into primary cells and escape from endosome/lysosome sequestration. We report that TALEN nanocapsules can effectively mutagenize the HIV-1 proviral DNA integrated into two primary HIV-1 reservoir cells─T cells and macrophages, such that replication and/or reactivation from latency is aborted. We envision that this study provides a useful platform to deliver a wide range of DNA-modifying enzymes for effective HIV therapy.</p>","PeriodicalId":2,"journal":{"name":"ACS Applied Bio Materials","volume":" ","pages":"1227-1239"},"PeriodicalIF":4.6,"publicationDate":"2025-02-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143070714","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Biomimetic Molecularly Imprinted Nanogels for the Recognition of Spike Glycoproteins.","authors":"Iulia Elena Neblea, Tanţa-Verona Iordache, Andrei Sarbu, Anita-Laura Chiriac, Ana-Mihaela Gavrila, Bogdan Trica, Iuliana Elena Biru, Iuliana Caras, Mircea Teodorescu, François-Xavier Perrin, Anamaria Zaharia","doi":"10.1021/acsabm.4c01757","DOIUrl":"https://doi.org/10.1021/acsabm.4c01757","url":null,"abstract":"<p><p>The rapid spread and mutation of SARS-CoV-2, the virus responsible for COVID-19, has set the foundation for extensive research into next-generation therapeutic strategies. A critical component of SARS-CoV-2 is the trimeric Spike (S) glycoprotein, which facilitates viral entry into host cells by interacting with the receptor-binding domain (RBD). To inhibit and block viral entry, we designed and developed molecularly imprinted synthetic nanogel antibodies (MIP-SNAs) that cap the Spike S1 RBD. This aims to provide a versatile, biosecure, and effective therapeutic tool for the prevention and treatment of SARS-CoV-2 infection. Herein, we employed reverse miniemulsion polymerization to synthesize MIP-SNAs using poly(ethylene glycol) diacrylate (PEGDA), a nontoxic, nonimmunogenic and FDA-approved polymer, able to interact noncovalently with the functional groups of template Spike S1 RBD. In addition, the formulation of MIP-SNAs was based on a preliminary investigation of protein conformation by circular dichroism. Characterization of the SNAs was conducted using several techniques to investigate the chemical structure, thermal stability, size, and morphology. Under optimal conditions, the MIP-SNAs exhibited high specificity, with rebinding capacities up to 6-fold higher compared to the control nonimprinted synthetic nanogel antibodies. MIP-SNAs also demonstrated notable selectivity toward the SARS-CoV-2 Spike S1 RBD protein compared to the structural resembling Spike proteins of Bat-CoV, while cytocompatibility assays confirmed the biocompatible character of the SNAs. Given the excellent features of the recently developed MIP-SNAs, we are one step closer to finding efficient but also patient-friendly prevention and treatment solutions for SARS-CoV-2 infection. Beyond immediate applications, this technology offers the potential for broader diagnostic and therapeutic uses against related viral pathogens.</p>","PeriodicalId":2,"journal":{"name":"ACS Applied Bio Materials","volume":" ","pages":""},"PeriodicalIF":4.6,"publicationDate":"2025-02-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143439265","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"3D-Printed PCL/SrHA@DFO Bone Tissue Engineering Scaffold with Bone Regeneration and Vascularization Function.","authors":"Kai Chen, Liu Luo, Ruolan Tao, Muzi Li, Shuqi Qu, Xiaofang Wu, Xinyue Zhang, Haiyan Feng, Ziqiang Zhu, Dekun Zhang","doi":"10.1021/acsabm.4c01866","DOIUrl":"10.1021/acsabm.4c01866","url":null,"abstract":"<p><p>The application of a three-dimensional (3D)-printed biological functional scaffold in the repair of bone defects is a promising strategy. In this study, strontium-containing hydroxyapatite (SrHA) powder was synthesized by the hydrothermal method, and then poly(ε-caprolactone) (PCL)/HA and PCL/SrHA composite scaffolds were prepared by the high-temperature melt extrusion 3D printing technology. The basic physical and chemical properties, in vitro biological properties, osteogenesis, and angiogenesis abilities of the scaffold were studied. The results showed that HA and SrHA were uniformly embedded in the composite scaffold, and the scaffold exhibited a 3D interconnected porous structure and rough microsurface. The in vitro release curve showed that Sr²⁺ and Ca²⁺ were continuously released from the PCL/SrHA scaffold. In order to verify the performance of the composite scaffold in bone regeneration, the proliferation and osteogenic differentiation of mouse embryonic osteoblasts (MC3T3E1) grown on the scaffold were evaluated. The experimental results showed that the incorporation of SrHA significantly promoted cell proliferation. Compared with the PCL/HA scaffold, the PCL/SrHA scaffold could better promote cell osteogenic differentiation. Deferoxamine (DFO) was loaded on the surface of the PCL/SrHA scaffold. By studying the proliferation, angiogenesis, and expression of osteogenesis and angiogenesis-related genes of human umbilical vein endothelial cells (HUVECs) on PCL/SrHA@DFO scaffold, it was verified that DFO had the ability to promote angiogenesis. It could induce angiogenesis in vitro in combination with Sr²⁺. Therefore, we believe that the composite scaffold has potential application prospects in the field of bone tissue engineering.</p>","PeriodicalId":2,"journal":{"name":"ACS Applied Bio Materials","volume":" ","pages":"1684-1698"},"PeriodicalIF":4.6,"publicationDate":"2025-02-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143062108","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Dithiocarbamate-Based Sequence-Defined Oligomers as Promising Membrane-Disrupting Antibacterial Agents: Design, Activity, and Mechanism.","authors":"Anna Jose, Shabin N Chathangad, Palliyil Sivadas, Debashis Barik, Karthika Kannan, Sovan Lal Das, Sushabhan Sadhukhan, Mintu Porel","doi":"10.1021/acsabm.4c01732","DOIUrl":"10.1021/acsabm.4c01732","url":null,"abstract":"<p><p>The emerging prevalence of antimicrobial resistance demands cutting-edge therapeutic agents to treat bacterial infections. We present a synthetic strategy to construct sequence-defined oligomers (SDOs) by using dithiocarbamate (DTC). The antibacterial activity of the synthesized library of SDOs was studied using a Gram-positive <i>B. subtilis</i> and a Gram-negative <i>E. coli</i>. Among SDOs, <b>Dec</b> (with C₁₀ aliphatic chain) was found to be the most promising antibacterial agent exhibiting a minimum inhibitory concentration (MIC) of 3 μg/mL against <i>B. subtilis</i>. Structure-activity relationship studies led to a 400-fold improvement in the MIC within the SDO library. The mode of action of the SDOs was elucidated on a model system, where bacterial membranes mimicking giant unilamellar vesicles (GUVs) were exposed to the SDOs. Membrane disruption and pore formation were found to be the key mechanisms through which SDOs act. In addition, scanning electron microscopy (SEM) and confocal laser scanning microscopy analysis of <b>Dec</b>-treated bacteria confirmed the loss of cell membrane integrity. Finally, the hemolysis assay with SDOs revealed their excellent selectivity toward bacterial cells. Taken together, we developed a modular platform for the synthesis of SDOs having promising antibacterial activity and superior selectivity toward bacteria, with the membrane disruption mode of action confirmed via studies on the model GUV system and SEM analysis.</p>","PeriodicalId":2,"journal":{"name":"ACS Applied Bio Materials","volume":" ","pages":"1547-1558"},"PeriodicalIF":4.6,"publicationDate":"2025-02-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143062115","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effect of Gadolinium Doping on the Optical and Magnetic Properties of Red-Emitting Dual-Mode Carbon Dot-Based Probes for Magnetic Resonance Imaging.","authors":"Arina A Efimova, Zilya F Badrieva, Ekaterina A Brui, Mikhail D Miruschenko, Ivan A Aleinik, Alexander M Mitroshin, Olga V Volina, Aleksandra V Koroleva, Evgeniy V Zhizhin, Yingqi Liang, Songnan Qu, Elena V Ushakova, Evgeniia A Stepanidenko, Andrey L Rogach","doi":"10.1021/acsabm.4c01705","DOIUrl":"10.1021/acsabm.4c01705","url":null,"abstract":"<p><p>Bioimaging probes based on carbon dots (CDs) can become a useful replacement for existing commercial probes, benefiting clinical diagnostics. While the development of dual-mode CD-based probes for magnetic resonance imaging (MRI), which provides the ability for photoluminescence (PL) detection at the same time, is ongoing, several challenges have to be addressed. First, most of the CD-based probes still emit at shorter wavelengths (blue/green spectral range), which is harmful to biological objects or have very low PL intensity in the biological window of tissue transparency (red/near-infrared spectral range). Second, the relaxation characteristics of the proposed CD-based probes are still similar or only slightly larger than those of commercial contrast agents. Herein, we introduce a dual-mode probe allowing for simultaneous PL detection and MRI analysis, based on CDs doped with gadolinium ions (Gd³⁺) with a PL peak in the red (640 nm), a PL quantum yield up to 24%, and high longitudinal and transverse relaxivities reaching 25.76 and 40.57 L mmol^-1 s^-1, respectively. Moreover, Gd-doped CDs show good biocompatibility, which was studied on H9c2 and 4T1 cell lines with a cell viability above 70%. The developed Gd-doped red-emissive CDs can be utilized as efficient and nontoxic dual-mode probes for PL and MRI measurements carried out simultaneously.</p>","PeriodicalId":2,"journal":{"name":"ACS Applied Bio Materials","volume":" ","pages":"1493-1502"},"PeriodicalIF":4.6,"publicationDate":"2025-02-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143062121","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}