ACS Applied Bio Materials最新文献_第5页

Biocompatible OFETs for Selective and Real-Time Bacterial Detection Using BSA and Lysozyme Layers.

IF 4.6

ACS Applied Bio Materials Pub Date : 2025-04-03 DOI: 10.1021/acsabm.4c01618

Po-Hsiang Fang, Guan-Xu Chen, Shuying Wang, Ching-Hao Teng, Wen-Chun Huang, Horng-Long Cheng, Wei-Yang Chou

{"title":"Biocompatible OFETs for Selective and Real-Time Bacterial Detection Using BSA and Lysozyme Layers.","authors":"Po-Hsiang Fang, Guan-Xu Chen, Shuying Wang, Ching-Hao Teng, Wen-Chun Huang, Horng-Long Cheng, Wei-Yang Chou","doi":"10.1021/acsabm.4c01618","DOIUrl":"https://doi.org/10.1021/acsabm.4c01618","url":null,"abstract":"In the realms of modern medicine and environmental monitoring, there is an escalating demand for bacterial detection technologies that are rapid, precise, and highly sensitive. Conventional methods, however, are often hindered by their time-intensive nature, procedural complexity, and reliance on specialized laboratory equipment. This study introduces an innovative approach utilizing bovine serum albumin (BSA) as the dielectric layer and lysozyme (LYZ) as the bacterial sensing layer in organic field-effect transistors (OFETs). The combination of BSA and LYZ enhances both biocompatibility and detection sensitivity, enabling precise differentiation between Gram-positive and Gram-negative bacteria. BSA not only stabilizes the electrical performance of the OFET but also offers biodegradability and water solubility, contributing to environmental sustainability. These biocompatible OFETs can accurately detect bacterial concentrations ranging from 104 to 108 CFU/mL, with real-time response capabilities via multispike measurements. This research represents a significant step forward in the development of advanced, portable biosensors for use in complex biological environments, advancing bacterial detection technology.","PeriodicalId":2,"journal":{"name":"ACS Applied Bio Materials","volume":" ","pages":""},"PeriodicalIF":4.6,"publicationDate":"2025-04-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143778549","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Nonfouling Core–Shell Microneedle for Sequential and Sustained Drug Release: Enhancing Synergistic Photothermal Chemotherapy in Melanoma Treatment

IF 4.6

ACS Applied Bio Materials Pub Date : 2025-04-03 DOI: 10.1021/acsabm.5c0012010.1021/acsabm.5c00120

Dongyu Huang, Qingyu Yu*, Kaiyue Yang, Xiuqiang Li, Chenlu Huang, Xinyu Yang, Chaoxiong Wu, Cheng Cao, Linhua Zhang, Dunwan Zhu* and Junjie Li*,

{"title":"Nonfouling Core–Shell Microneedle for Sequential and Sustained Drug Release: Enhancing Synergistic Photothermal Chemotherapy in Melanoma Treatment","authors":"Dongyu Huang, Qingyu Yu*, Kaiyue Yang, Xiuqiang Li, Chenlu Huang, Xinyu Yang, Chaoxiong Wu, Cheng Cao, Linhua Zhang, Dunwan Zhu* and Junjie Li*, ","doi":"10.1021/acsabm.5c0012010.1021/acsabm.5c00120","DOIUrl":"https://doi.org/10.1021/acsabm.5c00120https://doi.org/10.1021/acsabm.5c00120","url":null,"abstract":"Melanoma is a highly aggressive and metastatic malignancy, where current treatment methods often result in damage to healthy tissues, suboptimal therapeutic outcomes, and immune-related side effects. Microneedles, as a drug delivery system, offer advantages such as localized administration, minimal invasiveness, and high delivery efficiency. In this study, we first synthesized tetradecyl-thiol-grafted PAMAM dendrimers, which significantly enhanced cellular uptake and enabled sustained release of doxorubicin (DOX), improving cumulative drug release efficiency. Based on this, we developed a core–shell structured zwitterionic polymer-based microneedle delivery system. The outer shell, loaded with the photothermal agent indocyanine green (ICG), achieved precise photothermal therapy under near-infrared irradiation, effectively targeting melanoma tissues. The inner core, composed of a zwitterionic polymer matrix, encapsulated DOX-loaded dendrimers, enabling controlled and prolonged drug release through gradual polymer swelling and dendrimer expansion. Experiments show that the microneedle drug delivery system based on PAMAM dendrimer grafted with tetradecyl mercaptan and zwitterionic polymer has excellent anti protein adsorption properties, and it can minimize the cytotoxicity of carrier and improve the efficiency of drug delivery. This system effectively inhibited tumor growth through synergistic photothermal-chemotherapy, reducing systemic toxicity and improving drug bioavailability. This microneedle platform provides a promising strategy for targeted and synergistic melanoma therapy, offering a high-efficiency and low-toxicity treatment alternative.","PeriodicalId":2,"journal":{"name":"ACS Applied Bio Materials","volume":"8 4","pages":"3356–3374 3356–3374"},"PeriodicalIF":4.6,"publicationDate":"2025-04-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143851171","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Biocompatible OFETs for Selective and Real-Time Bacterial Detection Using BSA and Lysozyme Layers

IF 4.6

ACS Applied Bio Materials Pub Date : 2025-04-03 DOI: 10.1021/acsabm.4c0161810.1021/acsabm.4c01618

Po-Hsiang Fang, Guan-Xu Chen, Shuying Wang, Ching-Hao Teng, Wen-Chun Huang, Horng-Long Cheng and Wei-Yang Chou*,

{"title":"Biocompatible OFETs for Selective and Real-Time Bacterial Detection Using BSA and Lysozyme Layers","authors":"Po-Hsiang Fang, Guan-Xu Chen, Shuying Wang, Ching-Hao Teng, Wen-Chun Huang, Horng-Long Cheng and Wei-Yang Chou*, ","doi":"10.1021/acsabm.4c0161810.1021/acsabm.4c01618","DOIUrl":"https://doi.org/10.1021/acsabm.4c01618https://doi.org/10.1021/acsabm.4c01618","url":null,"abstract":"In the realms of modern medicine and environmental monitoring, there is an escalating demand for bacterial detection technologies that are rapid, precise, and highly sensitive. Conventional methods, however, are often hindered by their time-intensive nature, procedural complexity, and reliance on specialized laboratory equipment. This study introduces an innovative approach utilizing bovine serum albumin (BSA) as the dielectric layer and lysozyme (LYZ) as the bacterial sensing layer in organic field-effect transistors (OFETs). The combination of BSA and LYZ enhances both biocompatibility and detection sensitivity, enabling precise differentiation between Gram-positive and Gram-negative bacteria. BSA not only stabilizes the electrical performance of the OFET but also offers biodegradability and water solubility, contributing to environmental sustainability. These biocompatible OFETs can accurately detect bacterial concentrations ranging from 104 to 108 CFU/mL, with real-time response capabilities via multispike measurements. This research represents a significant step forward in the development of advanced, portable biosensors for use in complex biological environments, advancing bacterial detection technology.","PeriodicalId":2,"journal":{"name":"ACS Applied Bio Materials","volume":"8 4","pages":"2867–2874 2867–2874"},"PeriodicalIF":4.6,"publicationDate":"2025-04-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://pubs.acs.org/doi/epdf/10.1021/acsabm.4c01618","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143851246","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Porous Sodium Carboxymethyl Starch Microspheres for Hemostasis and Skin Wound Healing.

IF 4.6

ACS Applied Bio Materials Pub Date : 2025-04-03 DOI: 10.1021/acsabm.4c01933

Qing Zhou, Wenjie Chen, Han Wang, Cuicui Wu, Qianqian Zhu, Lei Luo, Xiao Zheng, Chenglong Yu, Aijun Guo, Jianjin Wang, Shunqing Tang

引用次数: 0

Nonfouling Core-Shell Microneedle for Sequential and Sustained Drug Release: Enhancing Synergistic Photothermal Chemotherapy in Melanoma Treatment.

IF 4.6

ACS Applied Bio Materials Pub Date : 2025-04-03 DOI: 10.1021/acsabm.5c00120

Dongyu Huang, Qingyu Yu, Kaiyue Yang, Xiuqiang Li, Chenlu Huang, Xinyu Yang, Chaoxiong Wu, Cheng Cao, Linhua Zhang, Dunwan Zhu, Junjie Li

{"title":"Nonfouling Core-Shell Microneedle for Sequential and Sustained Drug Release: Enhancing Synergistic Photothermal Chemotherapy in Melanoma Treatment.","authors":"Dongyu Huang, Qingyu Yu, Kaiyue Yang, Xiuqiang Li, Chenlu Huang, Xinyu Yang, Chaoxiong Wu, Cheng Cao, Linhua Zhang, Dunwan Zhu, Junjie Li","doi":"10.1021/acsabm.5c00120","DOIUrl":"https://doi.org/10.1021/acsabm.5c00120","url":null,"abstract":"Melanoma is a highly aggressive and metastatic malignancy, where current treatment methods often result in damage to healthy tissues, suboptimal therapeutic outcomes, and immune-related side effects. Microneedles, as a drug delivery system, offer advantages such as localized administration, minimal invasiveness, and high delivery efficiency. In this study, we first synthesized tetradecyl-thiol-grafted PAMAM dendrimers, which significantly enhanced cellular uptake and enabled sustained release of doxorubicin (DOX), improving cumulative drug release efficiency. Based on this, we developed a core-shell structured zwitterionic polymer-based microneedle delivery system. The outer shell, loaded with the photothermal agent indocyanine green (ICG), achieved precise photothermal therapy under near-infrared irradiation, effectively targeting melanoma tissues. The inner core, composed of a zwitterionic polymer matrix, encapsulated DOX-loaded dendrimers, enabling controlled and prolonged drug release through gradual polymer swelling and dendrimer expansion. Experiments show that the microneedle drug delivery system based on PAMAM dendrimer grafted with tetradecyl mercaptan and zwitterionic polymer has excellent anti protein adsorption properties, and it can minimize the cytotoxicity of carrier and improve the efficiency of drug delivery. This system effectively inhibited tumor growth through synergistic photothermal-chemotherapy, reducing systemic toxicity and improving drug bioavailability. This microneedle platform provides a promising strategy for targeted and synergistic melanoma therapy, offering a high-efficiency and low-toxicity treatment alternative.","PeriodicalId":2,"journal":{"name":"ACS Applied Bio Materials","volume":" ","pages":""},"PeriodicalIF":4.6,"publicationDate":"2025-04-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143778559","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Porous Sodium Carboxymethyl Starch Microspheres for Hemostasis and Skin Wound Healing

IF 4.6

ACS Applied Bio Materials Pub Date : 2025-04-03 DOI: 10.1021/acsabm.4c0193310.1021/acsabm.4c01933

Qing Zhou, Wenjie Chen, Han Wang, Cuicui Wu, Qianqian Zhu, Lei Luo, Xiao Zheng, Chenglong Yu, Aijun Guo, Jianjin Wang* and Shunqing Tang*,

{"title":"Porous Sodium Carboxymethyl Starch Microspheres for Hemostasis and Skin Wound Healing","authors":"Qing Zhou, Wenjie Chen, Han Wang, Cuicui Wu, Qianqian Zhu, Lei Luo, Xiao Zheng, Chenglong Yu, Aijun Guo, Jianjin Wang* and Shunqing Tang*, ","doi":"10.1021/acsabm.4c0193310.1021/acsabm.4c01933","DOIUrl":"https://doi.org/10.1021/acsabm.4c01933https://doi.org/10.1021/acsabm.4c01933","url":null,"abstract":"An effective and rapid hemostatic material with flexible properties for clinical wound dressings is still an unmet need. Herein, a porous sodium carboxymethyl starch (CMS–Na-P) hemostatic microsphere was successfully fabricated through polysaccharide fluffy aggregate (PSFA) technology with a facile and low-cost process. CMS–Na-P exhibited rapid water absorption capabilities alongside favorable cytocompatibility and hemocompatibility. Additionally, CMS–Na-P could absorb red blood cells (RBCs), adhere to and activate platelets, and shorten clotting time in vitro. More importantly, its good in vivo hemostatic ability was further demonstrated against hemorrhage in rat liver and tail, pig superficial skin, superficial body vein, superficial abdominal vein, and femoral artery. Meanwhile, in a rat full-thickness skin defect model, CMS–Na-P could enhance wound healing through accelerated epidermal regeneration and collagen deposition. These properties make CMS–Na-P a promising candidate for treating bleeding and full-thickness wounds.","PeriodicalId":2,"journal":{"name":"ACS Applied Bio Materials","volume":"8 4","pages":"3076–3085 3076–3085"},"PeriodicalIF":4.6,"publicationDate":"2025-04-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143851248","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Single-Molecule Mapping Landscape of Multivalent Antibody-DNA Framework Conjugates.

IF 4.6

ACS Applied Bio Materials Pub Date : 2025-04-02 DOI: 10.1021/acsabm.5c00346

Qinglin Xia, Mo Zhou, Xia Liu, Yue Wang, Kai Jiao, Bin Li, Lihua Wang, Linjie Guo, Jiang Li

{"title":"Single-Molecule Mapping Landscape of Multivalent Antibody-DNA Framework Conjugates.","authors":"Qinglin Xia, Mo Zhou, Xia Liu, Yue Wang, Kai Jiao, Bin Li, Lihua Wang, Linjie Guo, Jiang Li","doi":"10.1021/acsabm.5c00346","DOIUrl":"https://doi.org/10.1021/acsabm.5c00346","url":null,"abstract":"Patterned assembly of multivalent antibody complexes using DNA nanostructure templates holds the potential for advancing studies of cellular signaling and smart theranostic applications. However, evaluating the heterogeneity in protein conjugation efficiency at distinct sites on DNA templates remains challenging. Here, we utilize atomic force microscopy to measure the coupling of antibodies at various positions on two-dimensional rectangular DNA origami frameworks at the single-molecule level, generating spatial maps of antibody binding efficiencies across the structures. We observe that a discrete distribution of docking sites (spacing of at least 18 nm) on the framework leads to a progressive decrease in the antibody coupling efficiency from the periphery toward the center. In contrast, a continuous distribution of docking sites (spacing of ∼10 nm) results in a higher efficiency at the center relative to the periphery. We reason that the two opposing trends result from trade-offs among Coulombic repulsion, steric hindrance, and multivalent cooperative effects. This study presents a quantitative evaluation tool for protein-DNA framework conjugates, providing insights into optimizing DNA framework-based systems for improved precision in diagnostics and therapeutic applications.","PeriodicalId":2,"journal":{"name":"ACS Applied Bio Materials","volume":" ","pages":""},"PeriodicalIF":4.6,"publicationDate":"2025-04-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143770684","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Single-Molecule Mapping Landscape of Multivalent Antibody-DNA Framework Conjugates

IF 4.6

ACS Applied Bio Materials Pub Date : 2025-04-02 DOI: 10.1021/acsabm.5c0034610.1021/acsabm.5c00346

Qinglin Xia, Mo Zhou, Xia Liu, Yue Wang, Kai Jiao, Bin Li, Lihua Wang, Linjie Guo* and Jiang Li*,

{"title":"Single-Molecule Mapping Landscape of Multivalent Antibody-DNA Framework Conjugates","authors":"Qinglin Xia, Mo Zhou, Xia Liu, Yue Wang, Kai Jiao, Bin Li, Lihua Wang, Linjie Guo* and Jiang Li*, ","doi":"10.1021/acsabm.5c0034610.1021/acsabm.5c00346","DOIUrl":"https://doi.org/10.1021/acsabm.5c00346https://doi.org/10.1021/acsabm.5c00346","url":null,"abstract":"Patterned assembly of multivalent antibody complexes using DNA nanostructure templates holds the potential for advancing studies of cellular signaling and smart theranostic applications. However, evaluating the heterogeneity in protein conjugation efficiency at distinct sites on DNA templates remains challenging. Here, we utilize atomic force microscopy to measure the coupling of antibodies at various positions on two-dimensional rectangular DNA origami frameworks at the single-molecule level, generating spatial maps of antibody binding efficiencies across the structures. We observe that a discrete distribution of docking sites (spacing of at least 18 nm) on the framework leads to a progressive decrease in the antibody coupling efficiency from the periphery toward the center. In contrast, a continuous distribution of docking sites (spacing of ∼10 nm) results in a higher efficiency at the center relative to the periphery. We reason that the two opposing trends result from trade-offs among Coulombic repulsion, steric hindrance, and multivalent cooperative effects. This study presents a quantitative evaluation tool for protein–DNA framework conjugates, providing insights into optimizing DNA framework-based systems for improved precision in diagnostics and therapeutic applications.","PeriodicalId":2,"journal":{"name":"ACS Applied Bio Materials","volume":"8 4","pages":"3552–3559 3552–3559"},"PeriodicalIF":4.6,"publicationDate":"2025-04-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143851166","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Structural Design of Biodegradable Mg Gastrointestinal Anastomosis Staples for Corrosion and Mechanical Strength Analysis.

IF 4.6

ACS Applied Bio Materials Pub Date : 2025-04-01 DOI: 10.1021/acsabm.5c00143

Lin Mao, Xue Cai, Zhongxin Hu, Yujie Zhou, Zhiwei Dai, Yilong Chen, Hua Huang, Rui Zan, Chengli Song

{"title":"Structural Design of Biodegradable Mg Gastrointestinal Anastomosis Staples for Corrosion and Mechanical Strength Analysis.","authors":"Lin Mao, Xue Cai, Zhongxin Hu, Yujie Zhou, Zhiwei Dai, Yilong Chen, Hua Huang, Rui Zan, Chengli Song","doi":"10.1021/acsabm.5c00143","DOIUrl":"https://doi.org/10.1021/acsabm.5c00143","url":null,"abstract":"Magnesium (Mg) and its alloys, as next-generation materials for anastomosis staples, offer promising advantages such as biodegradability, biocompatibility, and reduced risk of long-term complications compared to traditional titanium materials. However, the performance of biodegradable staples is highly dependent on their structure. In this study, a biodegradable high-purity (HP) Mg staple with an optimized structure intended for small intestine anastomosis was developed and evaluated in vitro. The designed staple, with a diameter of 0.3 mm, featured an interior angle of 100° and a height of 3.8 mm. This design exhibited a maximum effective stress of approximately 170 MPa and an effective strain of 1.63. The staple could maintain structural integrity without fracture after 7 days of in vitro corrosion testing and exhibited a relatively high burst pressure of approximately 54.70 ± 2.51 mmHg. These findings indicate that the newly designed HP Mg staple combines superior corrosion resistance and anastomosis strength, confirming its potential for clinical application.","PeriodicalId":2,"journal":{"name":"ACS Applied Bio Materials","volume":" ","pages":""},"PeriodicalIF":4.6,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143750037","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Development of Electrospun Nerve Guidance Conduits by a Milk-Derived Protein with Biodegradable Polymers for Peripheral Nerve Regeneration.

IF 4.6

ACS Applied Bio Materials Pub Date : 2025-04-01 DOI: 10.1021/acsabm.4c02000

Taeoh Kim, Jin Jeon, Min Suk Lee, Jin Hee Park, Youngdoo Chung, Hee Seok Yang

{"title":"Development of Electrospun Nerve Guidance Conduits by a Milk-Derived Protein with Biodegradable Polymers for Peripheral Nerve Regeneration.","authors":"Taeoh Kim, Jin Jeon, Min Suk Lee, Jin Hee Park, Youngdoo Chung, Hee Seok Yang","doi":"10.1021/acsabm.4c02000","DOIUrl":"https://doi.org/10.1021/acsabm.4c02000","url":null,"abstract":"Bioactive and biodegradable fibrous conduits consisting of well-organized microfibers with longitudinal grooves on the fiber surface were prepared by electrospinning for nerve guidance conduit (NGC) application. Tubular constructs with uniaxially aligned topographical cues have great potential to enhance axonal regeneration and are needed to bridge large gaps between proximal and distal nerves. In this study, we developed electrospun NGCs using milk-derived casein protein (MDP) with biodegradable polycaprolactone and polylactic-co-glycolic acid. We designed and fabricated a biodegradable polymer for random fiber (RF), aligned fiber (AF), random fiber with MDP (MDP-RF), and aligned the fiber with MDP (MDP-AF) by using electrospinning. We hypothesized that topographically defined NGC as MDP-AF NGC would enhance axonal outgrowth by topographical cues and chemoattraction of the bioactive peptide in MDP for macrophage migration. The in vitro MDP-AF NGC results showed not only the promotion of a guidance effect on Schwann cell migration and macrophage polarization but also the enhancement of PC12 cell neurite outgrowth. Additionally, we demonstrated that the synergetic effects of the MDP-AF NGC enhanced the regeneration of injured sciatic nerves. To confirm the effect of MDP-AF NGC, we implanted it into a rat sciatic nerve (10 mm defect). The walking track analysis for sciatic function, electrophysiological test, gastrocnemius muscle weight, and histological and immunohistological analyses indicated that MDP-AF NGC effectively improved sciatic nerve regeneration compared with other groups at 4 and 8 weeks. Herein, we evolutionally developed MDP-AF NGC with geometric and chemotactic stimuli using an electrospinning method combined with a biocompatible synthetic polymer and bioactive casein protein.","PeriodicalId":2,"journal":{"name":"ACS Applied Bio Materials","volume":" ","pages":""},"PeriodicalIF":4.6,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143762496","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0