ACS Applied Bio Materials最新文献

{"title":"Multifunctional Core-Shell Cobalt Oxide @ Carbon Nanodot Hybrid Conjugates for Imaging and Targeting A549 Cells.","authors":"Anitha Jayapalan, Frank Tukur, Mahsa Azami, Mengxin Liu, Jianjun Wei","doi":"10.1021/acsabm.5c00343","DOIUrl":"10.1021/acsabm.5c00343","url":null,"abstract":"<p><p>The advent of research using drug-delivery vehicles with nanoparticles (NPs) in treating and diagnosing lung cancer has created a potential development in cancer therapeutics. Using certain NP-based compositions, specifically hybrid NPs, the cancer cells could be detected with enhanced fluorescence ability and treated using targeted drug release while minimizing adverse effects. A modified microwave-based synthesis approach was used in this study to synthesize spherical core-shell hybrid cobalt oxide carbon nanodot (Co₃O₄@CND) NPs of a smaller size of around 20 nm. Four different targeting ligands─folic acid, heparin, PEGylated silica (SiO₂), and transferrin─and the anticancer drug doxorubicin (DOX) were conjugated to the hybrid NPs, and their physicochemical characterizations were evaluated for their applications. The bioimaging, antioxidant, biocompatibility, cancer-targeting ability, and anticancerous specificity effect of the hybrid NPs were examined using A549 (lung cancer) cells and compared with CNDs, Co₃O₄ NPs, and the ligand-conjugated NPs. The Co₃O₄@CND NPs demonstrated high fluorescence from their synergistic properties, leading to a better bioimaging ability in human cells. The Co₃O₄@CND hybrid NP-transferrin-DOX composite targeted 50% of A549 cells with a much less adverse effect on EAhy926 (endothelial) cells at the same concentrations. Increased anticancer activity of the Co₃O₄@CNDs and improved biocompatibility were achieved via a receptor-mediated active targeting approach using specific ligands, proving the potential multifunctional applications such as bioimaging, antioxidant, and anticancer activity. After transferrin conjugation, the NP composite is more anticancerous to A549 and shows decreased toxicity to EAhy926 cells. The outcomes, while in the early stage, suggest that the Co₃O₄@CND hybrid NPs with ligand conjugation are a potential approach to the development of a multifunctional theranostic agent.</p>","PeriodicalId":2,"journal":{"name":"ACS Applied Bio Materials","volume":" ","pages":"4981-4994"},"PeriodicalIF":4.6,"publicationDate":"2025-06-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12175123/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144232617","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Advancements in Using Polymeric Nanoparticles for Blood-Brain Barrier Penetration in Neurological Disorders.","authors":"Yuao Wu, Shehzahdi S Moonshi, Hang Thu Ta","doi":"10.1021/acsabm.4c01956","DOIUrl":"10.1021/acsabm.4c01956","url":null,"abstract":"<p><p>Neurological disorders and glioblastoma represent a significant global health burden, affecting billions of individuals and contributing to high rates of morbidity and mortality. A primary obstacle in treating these conditions is the presence of the blood-brain barrier (BBB), a protective barrier that restricts the entry of most therapeutic agents into the brain. Despite this challenge, advancements in nanotechnology, specifically the development of polymeric nanoparticles, offer promising solutions for overcoming the BBB. Key strategies include surface modifications like PEGylation to enhance circulation time, receptor-mediated targeting for specific brain cells, and stimuli-responsive nanoparticles that release drugs in response to pH or reactive oxygen species. Ultrasound-guided delivery, intranasal administration, and magnetic nanoparticle guidance further enhance targeted delivery, while multifunctional nanoparticles enable combination therapies. These nanoparticles, with their customizable properties, allow for targeted and sustained delivery of drugs to the central nervous system, providing new hope in the treatment of both neurodegenerative diseases and brain cancers. In this review, we explore recent strategies that exploit polymeric nanoparticles to improve drug delivery across the BBB, highlighting their potential in revolutionizing therapeutic approaches for neurological disorders.</p>","PeriodicalId":2,"journal":{"name":"ACS Applied Bio Materials","volume":" ","pages":"4416-4431"},"PeriodicalIF":4.6,"publicationDate":"2025-06-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143951004","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Exosome-Based Sensor: A Landmark of the Precision Cancer Diagnostic Era.","authors":"Swarup Sonar, Asmit Das, Lee Yeong Zher, Ram Narayanan Ravi, Eason Qi Zheng Kong, Rajib Dhar, Kumaran Narayanan, Sukhamoy Gorai, Vetriselvan Subramaniyan","doi":"10.1021/acsabm.5c00288","DOIUrl":"10.1021/acsabm.5c00288","url":null,"abstract":"<p><p>Extracellular vesicles are nanoscale vesicles released by a diversity of cells that mediate intercellular communication by transporting an array of biomolecules. They are gaining increasing attention in cancer research due to their ability to carry specific biomarkers. This characteristic makes them potentially useful for highly sensitive, noninvasive diagnostic procedures and more precise prognostic assessments. Consequently, EVs are emerging as a transformative tool in cancer treatment, facilitating early detection and personalized medicine. Despite significant progress, clinical implementation is hindered by challenges in EV isolation, purification, and characterization. However, developing advanced biosensor technologies offers promising solutions to these obstacles. This review highlights recent progress in biosensors for EV detection and analysis, focusing on various sensing modalities including optical, electrochemical, microfluidic, nanomechanical, and biological sensors. We also explore techniques for EV isolation, characterization, and analysis, such as electron microscopy, atomic force microscopy, nanoparticle tracking analysis, and single-particle analysis. Furthermore, the review critically assesses the challenges associated with EV detection and put forward future directions, aiming to usher in a cutting-edge era of precision medicine through advanced, sensor-based, noninvasive early cancer diagnosis by detecting EV-carried biomarkers.</p>","PeriodicalId":2,"journal":{"name":"ACS Applied Bio Materials","volume":" ","pages":"4489-4513"},"PeriodicalIF":4.6,"publicationDate":"2025-06-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143951028","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Multifunctional Layered HPMC/PCL-59S Bioactive Glass Patches for Improved <i>In Vivo</i> Wound Healing with Potent Anti-Inflammatory and Angiogenic Effects.","authors":"Elakkiya Krishnamoorthy, Gosala Radha, Balakumar Subramanian","doi":"10.1021/acsabm.5c00400","DOIUrl":"10.1021/acsabm.5c00400","url":null,"abstract":"<p><p>Effective wound healing requires multifunctional biomaterials that support rapid tissue regeneration while providing structural integrity, biocompatibility, and therapeutic functionality. The successful fabrication of stacked patches was achieved through spin coating and electrospinning techniques, ensuring precise layering and seamless integration of Hydroxypropyl Methylcellulose (HPMC), Polycaprolactone (PCL), and 59S Bioglass (BG). The homogeneous dissolution of HPMC and PCL in the trisolvent mixture played a crucial role in achieving a uniform solution, facilitating the formation of well-structured layers. This integration enhanced the composite's structural and functional properties, with FESEM revealing a fibrous morphology and distinct layer differentiation. Degradation studies showed consistent weight loss in CP, CPD, and stacked patches over time particularly during the first 3 days, highlighting their stability. The stacked mat exhibited desirable mechanical properties with distinct elastic, strain-hardening, and fracture regions, achieving a tensile strength of 6.14 MPa and sufficient flexibility. Rapid degradation of the CB patches within 1 day emphasized the necessity of layer integration. The stacked patches exhibited superior biocompatibility with a reduced hemolysis rate (0.282%) and sustained metformin release over 3 days, crucial for inflammation management and tissue regeneration. The combination of HPMC/bioglass and HPMC/PCL/metformin demonstrated significant anti-inflammatory effects, inhibiting COX, LOX, MPO, and iNOS activities while reducing nitrite levels. Additionally, assays indicated a proliferation rate exceeding 90%, enhanced cell viability, angiogenesis, and antibacterial activity underscoring the stacked patches potential for wound healing. The combined attributes of structural stability, biocompatibility, efficient drug release, and anti-inflammatory efficacy represent a notable advancement in wound care with the potential to expedite the healing process. The <i>in vivo</i> studies demonstrated that the stacked patches significantly expedited wound closure, leading to full healing within 14 days. Histological evaluation evidently revealed enhanced tissue regeneration, characterized by rapid re-epithelialization, enhanced collagen formation, as well as increased vascularization, while also displaying a notable reduction in inflammation. Moreover, the lack of histopathological abnormalities in the examined organs obviously confirms their biocompatibility, reinforcing their suitability as a promising multifunctional biomaterial for advanced wound healing applications.</p>","PeriodicalId":2,"journal":{"name":"ACS Applied Bio Materials","volume":" ","pages":"5044-5066"},"PeriodicalIF":4.6,"publicationDate":"2025-06-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144074839","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Porous Silica Nanoparticle Entrapped Small Gd(III) and Mn(II) Complexes as MRI Contrast Agents.","authors":"Geetanjali Deka, Riya Mallik, Ismile Sk, Chandan Mukherjee","doi":"10.1021/acsabm.5c00747","DOIUrl":"10.1021/acsabm.5c00747","url":null,"abstract":"<p><p>Among the <i>in vivo</i> imaging techniques, magnetic resonance imaging (MRI) provides soft-tissue images with high spatial resolution without using any harmful ionizing radiation. Prior to <i>in vivo</i> imaging, the administration of a bolus injection of paramagnetic species, coined as contrast agents (CAs), has become almost routine to facilitate conspicuous imaging in a relatively short measurement period. The contrast agents are mainly small Gd(III)-complexes of macrocyclic and acyclic organic ligands with polar pendant arms. Nonetheless, reports on some adverse effects due to the accumulation of bare Gd(III) ions in the human body from the used gadolinium-based contrast agents necessitate extensive investigations on Mn(II)-complexes to engender potential alternatives. While thermodynamically stable and kinetically inert Mn-complexes with inner-sphere water molecule(s) have been developed and tested as CAs, the enhancement in the relaxivity value beyond 3.5 mM^-1 s^-1 has been intriguing. This review discloses the recent strategies for incorporating paramagnetic small Gd(III) and Mn(II) complexes within the porous nanosystems, the physicochemical properties, and stability and contrast efficiency improvement after confinement. The generation of \"smart\" and environmentally responsive contrasting probes by incorporating appropriate functional groups onto the surface of the robust nanosystems is also presented herein.</p>","PeriodicalId":2,"journal":{"name":"ACS Applied Bio Materials","volume":" ","pages":"4592-4611"},"PeriodicalIF":4.6,"publicationDate":"2025-06-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144118338","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"<i>In Vitro</i> Evaluation of Antibacterial Properties of NIR-Light Responsive Alginate Hydrogels Embedding Polydopamine Nanoparticles.","authors":"Sarah Defrançois, Alexandre Barras, Mickaël Maton, Patrice Woisel, Feng Hildebrand, Nicolas Blanchemain, Rabah Boukherroub, Joël Lyskawa","doi":"10.1021/acsabm.5c00481","DOIUrl":"10.1021/acsabm.5c00481","url":null,"abstract":"<p><p>Bacterial infections are one of the most serious health problems worldwide and represent a significant threat to humans. In this article, we designed an injectable alginate-based hydrogel embedding polydopamine nanoparticles (nPDA) and applied it as a (nano)phototherapeutic agent and nanocarrier for photodynamic (PDT) and photothermal (PTT) therapies with the perspective of treating bacterial infections and overcoming microbial resistance. For this purpose, nPDA were functionalized with Chlorin e6 as a photosensitizer and embedded in an alginate hydrogel to apply the PDT treatment. The photothermal properties of nPDA were exploited for the \"on demand\" local release of antibiotics such as Ciprofloxacin (for Gram-negative bacteria) and Rifampicin (for Gram-positive bacteria) to address respectively <i>Escherichia coli</i> and <i>Staphylococcus</i>s <i>aureus</i> as these antimicrobial-resistant pathogens are commonly found in bacterial infections. <i>In vitro</i> experiments have shown that PDT and PTT treatments were both highly efficient for the treatment of <i>S. aureus</i>, leading to the complete eradication of this bacterium. On the contrary, PDT was less effective for treating <i>E. coli</i>, while PTT revealed an excellent antibacterial activity toward this pathogen.</p>","PeriodicalId":2,"journal":{"name":"ACS Applied Bio Materials","volume":" ","pages":"5147-5157"},"PeriodicalIF":4.6,"publicationDate":"2025-06-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144155241","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Accelerating Wound Healing through a Mechano-Electric Synergistic Conductive Hydrogel.","authors":"Yingying Nie, Cewen Hu, Xinyue Huang, Huajing Zeng, Zhilong Wang, Jiachen Liang, Jizeng Wang","doi":"10.1021/acsabm.5c00523","DOIUrl":"10.1021/acsabm.5c00523","url":null,"abstract":"<p><p>To address the challenge of achieving faster wound healing, we present an innovative approach using hydrogel wound dressings that leverage the mechano-electric synergistic effect. This method incorporates piezoelectric zinc oxide nanoparticles (ZnO NPs) and conductive carbon nanotubes (CNTs) into a thermosensitive poly(<i>N</i>-isopropylacrylamide) (PNIPAM) hydrogel matrix. The engineered hydrogel demonstrates exceptional mechanical strength, optimal swelling properties, enhanced antibacterial activity, and excellent biocompatibility and biosafety. Upon application to a wound site, the hydrogel undergoes temperature-induced centripetal contraction, which enhances the wound closure process. Moreover, the morphological changes in the hydrogel caused by self-contraction and alterations in skin shape can trigger a piezoelectric effect, generating stable and lasting bioelectric signals that promote fibroblast migration. Consequently, a wound approximately 1 cm² in size can nearly completely heal within 14 days, thanks to the hydrogel's multifaceted therapeutic potential, including anti-inflammatory effects, promotion of cell migration, induction of fibroblast-to-myofibroblast differentiation, and enhancement of angiogenesis. This breakthrough represents a significant advancement over conventional hydrogel wound dressings, offering considerable promise for clinical application.</p>","PeriodicalId":2,"journal":{"name":"ACS Applied Bio Materials","volume":" ","pages":"5183-5193"},"PeriodicalIF":4.6,"publicationDate":"2025-06-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144155245","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"In Silico Identification and Verification of the Anticancer Mechanism of TMBM-010 from <i>Oxytropis herba</i> with a Rational Delivery System Design.","authors":"Zhe Zhang, Hui Zheng, Jindian Fan, Yitong Wang, Yushan Zhu, Kelsang Norbo, Lin Wu, Bo Du, Qingying Zhang, Dexin Kong","doi":"10.1021/acsabm.5c00265","DOIUrl":"10.1021/acsabm.5c00265","url":null,"abstract":"<p><p>Patients diagnosed with gastric cancer often face poor prognoses and limited treatment options. Current therapies remain limited, resulting in significant adverse effects and suboptimal outcomes. Network pharmacology analysis suggests that TMBM-010, a natural compound, holds the potential to modulate key pathways in cancer progression. Through network pharmacological analysis, we identified the anticancer mechanisms of TMBM-010, including ROS induction, DNA damage, apoptosis, and inhibition of DNA repair pathways. To enhance the bioavailability and efficacy of TMBM-010, we developed TMBM-010-loaded nanoparticles (TNPs) and biomimetic nanoparticles (TNPs@RGD-CM) coated with gastric cancer cell membranes and RGD ligands. TNPs@RGD-CM demonstrated high stability, excellent biosafety, and a controlled release profile. In a gastric cancer xenograft model, TNPs@RGD-CM significantly improved the bioavailability, increased ROS generation, and enhanced anticancer effects. Our findings demonstrate that TNPs@RGD-CM augment TMBM-010's bioactivity <i>in vivo</i>, effectively targeting cancer cells and suppressing tumor-promoting pathways. These results suggest that TNPs@RGD-CM represent a promising nanomedicine strategy for gastric cancer treatment.</p>","PeriodicalId":2,"journal":{"name":"ACS Applied Bio Materials","volume":" ","pages":"4884-4897"},"PeriodicalIF":4.6,"publicationDate":"2025-06-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144179660","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Multifunctional Tandem Peptide Mediates Targeted siRNA Delivery to Ovarian Cancer Cells.","authors":"Kharimat Lora Alatise, Timothy Samec, Carleigh Coffin, Serena Gilmore, Anthony Hazelton, Ruxi Xia, Chloe Jones, Angela Alexander-Bryant","doi":"10.1021/acsabm.4c01545","DOIUrl":"10.1021/acsabm.4c01545","url":null,"abstract":"<p><p>Fusogenic peptides have been developed to enhance the delivery efficiency of nucleic acids such as small interfering RNAs (siRNAs). Fusogenic peptides can protect nucleic acids from degradation and facilitate endosomal escape, but their systemic delivery capabilities remain unrefined. Active targeting of delivery systems to overexpressed cell receptors can be used to enhance cell- and tissue-specific delivery of therapeutic payloads while reducing off-target and systemic effects. We aimed to develop a targeted fusogenic (tandem) peptide, LHRHR-DIV3W, capable of targeting the luteinizing hormone-releasing hormone receptor (LHRHR), which is overexpressed in ovarian cancer cells and tissues. Characterization studies revealed that our tandem peptide, LHRHR-DIV3W, formed monodisperse nanocomplexes that protected siRNAs in physiological environments. We also demonstrated receptor-specific internalization of peptide-siRNA nanocomplexes in ovarian cancer cell lines with upregulated LHRHR expression. Furthermore, we observed that the inclusion of both the fusogenic DIV3W sequence and the LHRHR-targeting sequences in the tandem peptide enhanced receptor-specific siRNA internalization in ovarian cancer cells by up to 40% compared with the LHRHR-targeting peptide alone, indicating the necessity of combining both peptide regions. Finally, we demonstrated significant silencing of <i>CSNK2A1,</i> an oncogene overexpressed in ovarian cancer, in the ovarian cancer cell line OVCAR3. Our findings establish the use of a tandem peptide with cell-targeting and membrane-disruptive abilities as a delivery platform for nucleic acid therapies.</p>","PeriodicalId":2,"journal":{"name":"ACS Applied Bio Materials","volume":" ","pages":"4612-4620"},"PeriodicalIF":4.6,"publicationDate":"2025-06-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144214313","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Emerging Technologies and Solutions for Chronic Wound Care and Diagnosis","authors":"Simon Matoori*,&nbsp; and ,&nbsp;Elisabeth Engel*,&nbsp;","doi":"10.1021/acsabm.5c0086510.1021/acsabm.5c00865","DOIUrl":"https://doi.org/10.1021/acsabm.5c00865https://doi.org/10.1021/acsabm.5c00865","url":null,"abstract":"","PeriodicalId":2,"journal":{"name":"ACS Applied Bio Materials","volume":"8 6","pages":"4413–4415 4413–4415"},"PeriodicalIF":4.6,"publicationDate":"2025-06-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144290316","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}