ACS Applied Bio Materials最新文献

{"title":"Plasmonic Enhanced Nonlinear Absorption of Tris(2-aminoethyl)amine (TREN) Functional Ag, Pt, and Pd Nanoparticle-GQDs Complexes and Their Evaluation as Potential Bioimaging Applications.","authors":"Bekir Asilcan Unlu, Esen Kirit, Dogantan Celik, Elif Akhuseyin Yildiz, Ahmet Karatay, Bahadir Boyacioglu, Hüseyin Ünver, Açelya Yilmazer, Mustafa Yıldız, Ayhan Elmali","doi":"10.1021/acsabm.5c00750","DOIUrl":"10.1021/acsabm.5c00750","url":null,"abstract":"<p><p>Tris (2-aminoethyl)amine (TREN) functionalized N-doped graphene quantum dots (N-GQDs) and their Ag, Pd, and Pt nanocomposites were synthesized via a green one-step method and comprehensively characterized using FT-IR, UV-vis, TEM, and EDX. Spectroscopic analysis revealed π-π* transitions of C═C bonds (245-250 nm) and <i>n</i>-π* transitions of C═O and C═N bonds (334-350 nm), elucidating the materials' optical properties. Photoluminescence studies revealed excitation wavelength-dependent emissions, indicating the presence of edge defect levels. Femtosecond transient absorption spectroscopy revealed a shortened excited-state lifetime upon incorporation of a metal atom into TREN-GQDs. Nonlinear absorption was explored by using the open-aperture Z-scan method, revealing enhanced performance upon incorporation of plasmonic nanoparticles. Ag-incorporated samples exhibited the highest NLA response due to plasmon-enhanced two-photon absorption. Notably, Pt-incorporated N-GQDs showed improved NLA and good biocompatibility with the intracellular fluorescence response, positioning them as promising candidates for bioimaging applications. Density functional theory (DFT) calculations, including gas-phase optimizations and aqueous simulations, confirmed alignment with experimental results, highlighting the enhanced stability and reactivity of nanocomposites. These findings highlight the potential of these materials in various applications, such as optical limiting, imaging, photovoltaics, and sensing applications.</p>","PeriodicalId":2,"journal":{"name":"ACS Applied Bio Materials","volume":" ","pages":"6261-6277"},"PeriodicalIF":4.6,"publicationDate":"2025-07-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12284890/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144525236","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Safe and Efficient Intracellular Release of SN38 via Lysosomal-Responsive SN38-G Loaded Liposomes.","authors":"Pierre-Alain Burnouf, Yu-Cheng Su, Shih-Hung Yang","doi":"10.1021/acsabm.5c00722","DOIUrl":"10.1021/acsabm.5c00722","url":null,"abstract":"<p><p>Liposomal formulations remain mostly suited for amphiphilic drugs of mild potency. This high selectivity challenges the formulation of potent chemotherapeutics that are preponderantly hydrophobic. Spontaneous accumulation of certain hydrophobic drugs within the lipid bilayer of liposomes leads to aggregation, destabilization, and inadequate drug retention. Here, we depict an alternative approach to load such hydrophobic drugs using SN38, the active form of CPT-11 (Irinotecan, Camptosar), through SN38 glucuronide (SN38-G), its hydrophilic glucuronide prodrug derivative. SN38-G was esterified in acidic methanol or ethanol to produce amphiphilic derivatives SN38-G^met and SN38-G^eth compatible with liposomal core encapsulation. By employing an internally alkaline pH, core-loaded SN38-G^met and SN38-G^eth underwent spontaneous hydrolysis, reverting into the hydrophilic SN38-G. This internal conversion resulted in sustained drug retention through lipid-bilayer containment of hydrophilic compounds. Loading above 60% drug-to-phospholipid molar ratio was attained, together with sustained retention over 15 days at 37 °C in simulated body fluids. Our <i>in vitro</i> assay demonstrated that SN38-G liposomes were activated to SN38 through a lysosomal beta-glucuronidase-dependent manner, inducing cytotoxicity. This delivery method could be applied to various potent and hydrophobic drugs with challenging delivery requirements.</p>","PeriodicalId":2,"journal":{"name":"ACS Applied Bio Materials","volume":" ","pages":"6231-6242"},"PeriodicalIF":4.6,"publicationDate":"2025-07-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12284848/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144590011","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Nanotubular Gradients on Titanium: High-Throughput Screening of Nanoscale Architectures of Variable Topographical Complexity.","authors":"Ryan Berthelot, Fabio Variola","doi":"10.1021/acsabm.5c00697","DOIUrl":"10.1021/acsabm.5c00697","url":null,"abstract":"<p><p>Advancements in cell-instructive biomaterials hinge on the precise design of their nanoscale topography, a critical factor in controlling cell-surface interactions. Nanofabrication techniques such as e-beam and nanoimprint lithography enable accurate nanopatterning on a wide range of materials. However, their limited applicability and scalability to medically relevant metals such as titanium, hinder the creation and modulation of precisely designed nanotopographies on metallic substrates to investigate structure-function relationships and clinical translation of nanotopographical surfaces for biomedical implants. In this context, anodization is a cost-effective, scalable method to nanopattern titanium and its alloys, producing arrays of TiO₂ nanotubes with precisely controlled diameters. Despite the significant advances in the understanding of how cells sense and respond to nanotubular surfaces, traditional diameter-focused research reliant on single-sized nanostructures restricts analysis to a narrow set of geometrical parameters and often overlook the spatial arrangement of nanotubes. To address these limitations, this study capitalizes on anodization to create scalable nanotubular gradients on titanium, introducing a high-throughput platform to explore the cellular response to a wide range of nanotopographical configurations within a single sample. Utilizing spatial metrics such as lacunarity, entropy, and fractal dimension, we characterized the structural complexity of the nanotubular surfaces, emphasizing geometrical considerations beyond the nanotube diameter in evaluating cellular response. In vitro assays with human MG63 osteoblastic cells revealed that more disordered, high-entropy regions significantly enhance cellular spreading and proliferation while promoting early osteogenic differentiation, evidenced by elevated RUNX2 and osteocalcin (OCN) expression. In contrast, mitochondrial activation and longer-term mineral deposition are elicited by more ordered nanotubular arrays. By streamlining the screening of nanotopographical features and enabling reproduction of user-selected designs as homogeneous surfaces, this gradient-based approach deepens mechanistic insights into structure-function relationships governing MG63 cell response to anodized titanium and offers a translatable framework for designing and evaluating nanotubular surfaces, shortening the gap between in vitro research and clinical applications.</p>","PeriodicalId":2,"journal":{"name":"ACS Applied Bio Materials","volume":" ","pages":"6177-6192"},"PeriodicalIF":4.6,"publicationDate":"2025-07-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144309236","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Retraction of \"Chitosan-Stabilized CuO Nanostructure-Functionalized UV-Crosslinked PVA/Chitosan Electrospun Membrane as Enhanced Wound Dressing\".","authors":"M Nuruzzaman Khan, M Tarik Arafat, Taslim Ur Rashid, Papia Haque, Mohammed Mizanur Rahman","doi":"10.1021/acsabm.5c01078","DOIUrl":"10.1021/acsabm.5c01078","url":null,"abstract":"","PeriodicalId":2,"journal":{"name":"ACS Applied Bio Materials","volume":" ","pages":"6469"},"PeriodicalIF":4.6,"publicationDate":"2025-07-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144473270","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Lactoferrin-Anchored Carboxymethyl Pullulan-MgO Nanocomposites for Targeted Delivery of Trans-Ferulic Acid: Physicochemical Characterization, In vitro and Ex vivo Studies.","authors":"Prakash Kumar Sirvi, Paul Gajanan Balaji, Amit Kumar, Md Imtiyaz Alam, Ankita Sharma, Nabanita Das, Awesh Kumar Yadav","doi":"10.1021/acsabm.5c00482","DOIUrl":"10.1021/acsabm.5c00482","url":null,"abstract":"<p><p>The design of efficacious nanotherapeutics for neurodegenerative disorders necessitates the development of precisely targeted delivery systems capable of transversing the blood-brain barrier (BBB) while sustaining therapeutic efficacy. Here, we introduce a novel brain-targeted nanocomposite system comprising carboxymethylated pullulan-magnesium oxide (CMP-MgO) matrix encapsulating trans-ferulic acid (TFA) and last surface-functionalized with lactoferrin (Lf) to facilitate receptor-mediated transcytosis. Comprehensive physicochemical characterization, including dynamic light scattering (DLS), proton-nuclear magnetic resonance (¹H NMR), fourier transform infrared (FT-IR), high resolution-transmission electron microscopy (HR-TEM), atomic force microscopy (AFM), powder X-ray diffraction (PXRD), thermogravimetric analysis (TGA), and circular dichroism (CD) spectroscopy, confirmed the structural integrity functionalization, and stability of the nanocomposites. DLS studies exhibited a hydrodynamic diameter of 386 ± 5.06 nm, a polydispersity index of 0.087 ± 0.008, and a zeta potential of -20.5 ± 0.19 mV of the Lf-TFA-CMP-MgO. Morphological analysis confirmed spherical particles with a smooth surface, and CD spectroscopy confirmed the perpetuation of the native structure of Lf after conjugation with the nanocomposite. The antioxidant assay highlighted significant free radical scavenging activity, reflecting the antioxidant potential of TFA and TFA-loaded nanocomposites. <i>In vitro</i> studies demonstrated excellent biocompatibility and enhanced cellular internalization due to Lf functionalization. Notably, the nanocomposite inhibited amyloid fibril formation by interacting with hen egg white lysozyme (HEWL). Furthermore, intranasal delivery achieved efficient nose-to-brain transport, with permeability of 415.45 μg/cm² at 6 h, highlighting its mucoadhesive properties and noninvasive therapeutic capability for Alzheimer's disease (AD).</p>","PeriodicalId":2,"journal":{"name":"ACS Applied Bio Materials","volume":" ","pages":"5757-5774"},"PeriodicalIF":4.6,"publicationDate":"2025-07-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144339685","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Nanobody Coupled with Au Nanoparticle Cluster to Detect Survivin Protein by Lateral Flow Immunoassay.","authors":"Yujiao Tang, Xiaomin Zhao, Zhenlin Nie, Bangshun He, Dawei Deng","doi":"10.1021/acsabm.5c00745","DOIUrl":"10.1021/acsabm.5c00745","url":null,"abstract":"<p><p>The monoclonal antibody (mAb) used in a traditional Lateral Flow Immunoassay (LFIA) has many limitations, particularly related to its poor affinity, specificity, and stability. In this study, a nanobody (VHH) was developed with improved tolerability and higher specificity for the target. The strong binding affinity between VHH and survivin was emphasized by simulated docking, demonstrating the feasibility and effectiveness of replacing mAb in the detection of urinary survivin based on LFIA. In addition, the superior performance of VHH compared with mAb under extreme conditions was highlighted. To further enhance the detection performance, VHH was conjugated with an Au nanoparticle cluster (AuNP cluster). The pore size structure of the AuNP cluster led to an increased abundance of AuNPs, which amplified the signal while providing adequate adsorption sites for VHH coupling, thereby achieving a synergistic enhancement in the sensitivity and specificity of the AuNP cluster-VHH probe. Consequently, the detection limit for survivin was established at 3.90 ng/mL, facilitating noninvasive detection and monitoring in early-stage bladder cancer and during post-treatment surveillance. This insight underscores the key role of VHH in enhancing the performance of LFIA and the potential of developing a promising detection tool based on urinary biomarkers.</p>","PeriodicalId":2,"journal":{"name":"ACS Applied Bio Materials","volume":" ","pages":"6252-6260"},"PeriodicalIF":4.6,"publicationDate":"2025-07-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144525235","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"","authors":"María E. Pérez,&nbsp;Javier E. Durantini,&nbsp;María E. Milanesio and Edgardo N. Durantini*,&nbsp;","doi":"","DOIUrl":"","url":null,"abstract":"","PeriodicalId":2,"journal":{"name":"ACS Applied Bio Materials","volume":"8 7","pages":"XXX-XXX XXX-XXX"},"PeriodicalIF":4.6,"publicationDate":"2025-07-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://pubs.acs.org/doi/pdf/10.1021/acsabm.5c00787","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144665342","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"","authors":"Hsiao-Yen Lee,&nbsp;Natesan Thirumalaivasan and Shu-Pao Wu*,&nbsp;","doi":"","DOIUrl":"","url":null,"abstract":"","PeriodicalId":2,"journal":{"name":"ACS Applied Bio Materials","volume":"8 7","pages":"XXX-XXX XXX-XXX"},"PeriodicalIF":4.6,"publicationDate":"2025-07-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://pubs.acs.org/doi/pdf/10.1021/acsabm.5c00645","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144665343","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"","authors":"Haritha Asokan-Sheeja,&nbsp;Debdatta Das,&nbsp;Jenny N. Nguyen,&nbsp;Jiazhu Xu,&nbsp;Md Tareque Hassan Mukut,&nbsp;Tung H. Chau,&nbsp;Joseph A. Buonomo*,&nbsp;Yi Hong* and He Dong*,&nbsp;","doi":"","DOIUrl":"","url":null,"abstract":"","PeriodicalId":2,"journal":{"name":"ACS Applied Bio Materials","volume":"8 7","pages":"XXX-XXX XXX-XXX"},"PeriodicalIF":4.6,"publicationDate":"2025-07-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://pubs.acs.org/doi/pdf/10.1021/acsabm.5c00897","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144665355","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"","authors":"Amany M. Sawy,&nbsp;Badawi Anis,&nbsp;Shuna Cui and Rabeay Y. A. Hassan*,&nbsp;","doi":"","DOIUrl":"","url":null,"abstract":"","PeriodicalId":2,"journal":{"name":"ACS Applied Bio Materials","volume":"8 7","pages":"XXX-XXX XXX-XXX"},"PeriodicalIF":4.6,"publicationDate":"2025-07-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://pubs.acs.org/doi/pdf/10.1021/acsabm.5c00452","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144665364","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}