ACS Applied Bio Materials最新文献

{"title":"A Peptide Hydrogel Responsive to Reactive Oxygen Species and pH for the Protection and Sustained Delivery of Insulin-like Growth Factor 1 in Osteoarthritis Treatment.","authors":"Dona Imanga Upamadi Edirisinghe, Ashmeet Singh, Michael Patrick Seitz, Alireza Jafar Esmaeili, Thalma Kabeyi Orado, Emma Herrero, Jennifer Jankowski, Precious Kemilembe Kairuki, Liam R Marshall, Era Jain, Olga V Makhlynets","doi":"10.1021/acsabm.5c00092","DOIUrl":"10.1021/acsabm.5c00092","url":null,"abstract":"<p><p>Osteoarthritis (OA) is the leading cause of disability in adults worldwide. This debilitating disease is associated with a relatively high concentration of reactive oxygen species (ROS) and reduced pH in the joints. In this regard, we devised a two-pronged approach to treating OA that combines ROS neutralization and stimuli-responsive drug delivery. Specifically, we developed a series of biocompatible, self-healing short peptide hydrogels that preserve their rheological properties in a wide range of biologically relevant salt concentrations. We evaluated the ROS-responsive release of a cartilage healing promoting growth factor (insulin-like growth factor 1, IGF-1) from these biomaterials. Furthermore, the ROS-responsive peptide hydrogel's ability to sequester ROS can potentially relieve disease-associated symptoms and minimize cell damage. The simplicity and effectiveness of this multistimuli-responsive smart biomaterial suggest promise in OA treatment.</p>","PeriodicalId":2,"journal":{"name":"ACS Applied Bio Materials","volume":" ","pages":""},"PeriodicalIF":4.6,"publicationDate":"2025-06-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144332034","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Self-Surfactant Poly-3hydroxybutyrate-<i>co</i>-3hydroxyhexanoate (PHBHHx) for the Preparation of Usnic Acid Loaded Antimicrobial Nanoparticles Using Nontoxic Chemicals.","authors":"Sara Alfano, Lorenzo Ceparano, Benedetta Brugnoli, Gianluca Forcina, Luca Pellegrino, Francesca Cecilia Lauta, Roberto Rusconi, Iolanda Francolini, Antonella Piozzi, Andrea Martinelli","doi":"10.1021/acsabm.5c00676","DOIUrl":"https://doi.org/10.1021/acsabm.5c00676","url":null,"abstract":"<p><p>Polyhydroxyalkanoates (PHAs) are naturally occurring polyesters with promising drug delivery applications. Their hydrophobicity enables lipophilic drug encapsulation, enhancing bioavailability but limiting colloidal stability and physiological compatibility. Surfactants crucially improve the nanoparticle dimensional stability, dispersion, wettability of hydrophobic matrices, and cellular interaction, yet conventional surfactants require additional purification and may pose physiological risks. Self-surfactant systems offer a sustainable alternative. Therefore, this research proposes a green chemical modification of PHAs to develop self-surfactant systems. Hydrophilic groups were introduced onto a poly-3-hydroxybutyrate-<i>co</i>-3-hydroxyhexanoate (PHBHHx) backbone via amidation using choline taurinate ([Ch][Tau]), a biocompatible ionic liquid. This approach eliminates the need for toxic reagents and complex purification. By precisely controlling the PHBHHx/[Ch][Tau] molar ratio, amphiphilic structures with varying hydrophobic tail lengths were produced, as confirmed by infrared spectroscopy and chromatographic analysis. Nanoparticles were fabricated through the emulsion-solvent evaporation method and employed to encapsulate the lipophilic and antimicrobial agent usnic acid. Dynamic light scattering highlighted the obtainment of stable colloidal suspensions with dimensions of 40-160 nm. Biological evaluations demonstrated the antimicrobial efficacy against planktonic <i>S. aureus</i> Newman strain and biofilm inhibition under fluidic conditions even for the unloaded nanoparticles. Additionally, the nanoparticles exhibited no cytotoxicity at concentrations ranging from 10 to 0.1 μg/mL while retaining antimicrobial activity, in contrast to the high cytotoxicity observed for free usnic acid. Overall, this approach offers a sustainable and scalable strategy to produce self-surfactant and intrinsically antimicrobial polymeric nanocarriers suitable for the systemic drug delivery of lipophilic compounds, smart implant coatings, and antibacterial topical formulations.</p>","PeriodicalId":2,"journal":{"name":"ACS Applied Bio Materials","volume":" ","pages":""},"PeriodicalIF":4.6,"publicationDate":"2025-06-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144323798","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Ophicalcitum-Inspired Microspheres as a Topical Hemostatic Agent.","authors":"Yang Liu, Fen Wan, Ruoyu Ba, Yimin Han, Ting Geng, Beihua Bao, Weifeng Yao, Wei-Wei Xiong, Li Zhang, Fang-Fang Cheng","doi":"10.1021/acsabm.5c00925","DOIUrl":"https://doi.org/10.1021/acsabm.5c00925","url":null,"abstract":"<p><p>Ophicalcitum, as an oral mineral in traditional Chinese medicine, has a long history of therapeutic use in hemostasis, and its main component is calcium carbonate, which has good biosafety, excellent structural properties, and low cost. Inspired by it, calcium carbonate microspheres with different particle sizes were synthesized using ethylene glycol and ultrapure water as solvents. Furthermore, luteolin was loaded onto calcium carbonate microspheres to prepare multifunctional hemostatic materials with healing-promoting effects. Experimental results have shown that calcium carbonate microspheres had excellent hemostatic properties without hemolytic, cytotoxic, and metabolic toxic effects and their hemostatic properties were independent of particle sizes. In addition, luteolin loaded on calcium carbonate microspheres promoted skin healing, indicating that these calcium carbonate microspheres were potential hemostatic materials.</p>","PeriodicalId":2,"journal":{"name":"ACS Applied Bio Materials","volume":" ","pages":""},"PeriodicalIF":4.6,"publicationDate":"2025-06-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144323796","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Tunable Drug Release through Varying Drug Affinities for Ocular Chronic Disease.","authors":"Hyeonah Lee, Serim Byun, Moonyoung Kim, Hyeokjung Kim, Hyeran Noh","doi":"10.1021/acsabm.5c00425","DOIUrl":"https://doi.org/10.1021/acsabm.5c00425","url":null,"abstract":"<p><p>Effective drug delivery is critical for the management of chronic diseases such as glaucoma, where sustained therapeutic levels can significantly enhance treatment outcomes. In this study, we present a Particles-on-a-Gel (PoG) system that leverages differential nanocarrier affinities to modulate drug release kinetics. By integrating poly(N-isopropylacrylamide) nanogels (pNIPAM) and silver nanoparticles (AgNPs), the PoG platform enables both controlled initial release and prolonged drug delivery. Isothermal titration calorimetry (ITC) was employed to quantitatively characterize the thermodynamic interactions between timolol maleate and the nanocarriers, revealing distinct binding modalities─hydrophobic interactions with pNIPAM and chemically driven binding with AgNPs. These findings underscore the role of thermodynamic tuning in optimizing drug-carrier interactions to enhance release profiles and retention. Furthermore, incorporation of the PoG system into a contact lens-based drug delivery platform demonstrated its translational potential, maintaining optical transparency while enabling sustained drug release. Overall, this work highlights the promise of thermodynamically guided nanocarrier design in developing patient-centric drug delivery systems for chronic disease management.</p>","PeriodicalId":2,"journal":{"name":"ACS Applied Bio Materials","volume":" ","pages":""},"PeriodicalIF":4.6,"publicationDate":"2025-06-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144323799","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Poly(d,l-lactide-<i>co</i>-glycolide) Nanoparticles Encapsulating Doxorubicin for Improved Treatment in Cholangiocarcinoma and Drug-Resistant Cells.","authors":"Pornpattra Rattanaseth, Kanlaya Katewongsa, Kitti Intuyod, Somchai Pinlaor, Raynoo Thanan, Chadamas Sakonsinsiri","doi":"10.1021/acsabm.5c00628","DOIUrl":"https://doi.org/10.1021/acsabm.5c00628","url":null,"abstract":"<p><p>Cholangiocarcinoma (CCA), a malignancy of the bile duct epithelium, represents a significant public health issue in the Greater Mekong Subregion, including Thailand. Its aggressive characteristics and late-stage diagnosis lead to poor prognosis and elevated mortality rates. Chemotherapy faces limitations, including the requirement for high and frequent dosages, low cellular uptake, and side effects. To address these challenges, poly(d,l-lactide-<i>co</i>-glycolide) (PLGA) nanoparticles (NPs) encapsulating doxorubicin (DOX), a chemotherapeutic drug, were developed via a modified nanoprecipitation technique. PLGA was chosen for its biocompatibility and controlled release properties, while the intrinsic fluorescence of DOX allowed cellular uptake monitoring. Among various formulations, Formulation A4 yielded uniform, smooth and spherical NPs with an average diameter of 341 nm, a surface charge of -23 mV, and a suitable encapsulation efficiency. DOX-PLGA NPs were characterized in terms of hydrodynamic diameter (D_h), morphology, heterogeneity of particle sizes, surface charge and surface functional groups, and encapsulation efficiency (EE). Blank NPs, prepared under identical conditions without DOX, were nonhemolytic and biocompatible. The <i>in vitro</i> release profile of the DOX-PLGA NPs showed a biphasic pattern, characterized by both a burst and sustained release, fitting the Korsmeyer-Peppas model. DOX was released more rapidly in an acidic environment compared to physiological pH. DOX-PLGA NPs exhibited greater cytotoxicity relative to free DOX in both KKU-213A and KKU-055 CCA cells, along with increased cellular uptake. In gemcitabine-resistant KKU-213B cells, DOX-PLGA NPs exhibited significantly enhanced cytotoxic effects. The prepared DOX-PLGA NPs demonstrated favorable physicochemical properties, enhanced drug delivery, and improved anticancer activity, highlighting their potential as an efficient DDS for CCA treatment.</p>","PeriodicalId":2,"journal":{"name":"ACS Applied Bio Materials","volume":" ","pages":""},"PeriodicalIF":4.6,"publicationDate":"2025-06-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144323797","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Bioinspired Silica Nanoparticles for Enhanced Adhesion and Retention of Biomolecules: A Sustainable and Green Strategy for Disease Management.","authors":"Anju Sangwan, Nitish Kumar, Neetu Singh","doi":"10.1021/acsabm.5c00529","DOIUrl":"https://doi.org/10.1021/acsabm.5c00529","url":null,"abstract":"<p><p>The growing significance of sustainable agriculture is underscored by the escalating global demand for food and environmental degradation. Continuously adapting plant pathogens presents a significant challenge to sustainable agriculture, leading to substantial global crop losses. <i>Botrytis cinerea</i> is widely distributed and poses a menace to a diverse range of economically important crops. Conventional delivery strategies for active molecules have a low utilization rate, leading to decreased bioavailability, thus reducing the overall efficacy. Utilizing stimulus-responsive carriers for biomolecule delivery into plants enhances efficiency against target pathogens, minimizes risks to nontarget organisms and the environment, and plays a crucial role in increasing active ingredient bioavailability, reducing application frequency. Here, we have prepared a nanobiopesticide composition with foliage-adhesive properties that shows release in pathological conditions, utilizing biocompatible Guar gum/Salicylic acid (GG/SA) as the encapsulating agent on silica nanoparticles (MSNPs) as the carrier, loaded with tannic acid (TA). In comparison to the naked biomolecule, the GG/SA encapsulation improves the deposition of tannic acid, and the contact angle on tomato leaves is decreased by 28°. The enhanced antiwashing efficiency of ^TanMSNP_GG/SA, compared to its naked biomolecule counterparts, can be attributed to low surface tension and van der Waals interactions. Different from free tannic acid, ^TanMSNP_GG/SA exhibited a responsive release in pathological conditions, leading to sustained and steady biomolecule release and prolonged persistence time. Moreover, the control efficacy of ^TanMSNP_GG/SA against <i>B. cinerea</i> was 94%. Also, they showed no cytotoxicity on NIH3T3 cells. This study anticipates improving the adhesion of biomolecules, maximizing the utilization efficiency with stimulus-responsive carriers, thereby addressing a significant application challenge in the field of agriculture, aligning with ecofriendly agricultural practices, presenting an economically viable option for farmers, and ensuring food safety.</p>","PeriodicalId":2,"journal":{"name":"ACS Applied Bio Materials","volume":" ","pages":""},"PeriodicalIF":4.6,"publicationDate":"2025-06-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144315479","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Plasmonic ELISA for Biomarker Detection: A Review of Mechanisms, Functionalization Strategies, and Emerging Modalities.","authors":"Chaudhary Ammar Shoukat, Maryam Tariq, Raja Muhammad Aqib, Muhammad Ali Tajwar, Rashid Iqbal","doi":"10.1021/acsabm.5c00738","DOIUrl":"https://doi.org/10.1021/acsabm.5c00738","url":null,"abstract":"<p><p>Plasmonic enzyme-linked immunosorbent assay (ELISA) effectively integrates noble metal nanostructures with traditional immunoassays, facilitating rapid, ultrasensitive, and multiplexed biomarker detection. By leveraging localized surface plasmon resonance modulations instigated by biocatalytic reactions and analyte binding, these assays achieve signal amplification through growth, etching, and aggregation mechanisms. Such methodologies significantly enhance detection limits by factors ranging from 10- to over 1000-fold, attaining sensitivity at the subpicogram per milliliter level. Robust surface functionalization methods, including electrostatic adsorption, covalent coupling, and affinity binding, ensure stable immobilization of antibodies while preserving the activity of the nanozymes. Incorporating advanced two-dimensional nanomaterials, such as graphene derivatives and MXenes, further augments the sensitivity (up to ∼200-fold), assay stability, and potential for miniaturization. Emerging modalities, including electrochemical techniques, microfluidics, photothermal methods, surface-enhanced infrared absorption (SEIRA), surface-enhanced Raman scattering, and CRISPR-enabled ELISA, extend the analytical versatility, multiplexing capabilities, and operational speed. Clinical trials, alongside real-world studies, substantiate the efficacy of plasmonic ELISA platforms in early cancer detection, diagnostic evaluation of infectious diseases, and monitoring cardiovascular biomarkers, demonstrating performance comparable to or exceeding that of traditional methodologies. Despite significant advancements, challenges persist with regard to assay standardization, multiplex integration, and large-scale manufacturing. This review presents a comprehensive overview of recent developments, identifies critical knowledge gaps, and outlines future perspectives to expedite the clinical translation of plasmonic ELISA technologies for precision medicine and global health applications.</p>","PeriodicalId":2,"journal":{"name":"ACS Applied Bio Materials","volume":" ","pages":""},"PeriodicalIF":4.6,"publicationDate":"2025-06-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144315484","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Inhibition of Phosphoglycerate Kinase 1 (PGK1) Decreases Neointimal Hyperplasia after Patch Angioplasty.","authors":"Peng Sun, Hao Cui, Changwei Ren, Chenzhen Xu, Chuanjie Yue, Jing'an Li, Yongqiang Lai","doi":"10.1021/acsabm.5c00589","DOIUrl":"https://doi.org/10.1021/acsabm.5c00589","url":null,"abstract":"<p><p>Neointimal hyperplasia is a known complication following aorta interventions. In this study, our hypothesis was that inhibiting phosphoglycerate kinase 1 (PGK1) could effectively reduce aortic neointimal hyperplasia in a rat model of abdominal aortic patch angioplasty. The role of the glycolytic pathway in patch angioplasty was analyzed by next-generation sequencing data, and the core role of PGK1 was found by differential gene analysis. The rats were allocated into two distinct groups: a control group that did not receive any supplementary treatment and a group treated with NG52, an inhibitor of PGK1, which was administered via a PLGA coating. Abdominal aortic patches were surgically implanted in the rats and subsequently harvested on the 14th day postimplantation for further analysis. Immunohistochemical analysis identified the presence of PGK1-positive cells within the neointima of the rat model subjected to abdominal aortic patch angioplasty. Importantly, the use of NG52 PLGA coating significantly decreased neointimal thickness (<i>p</i> < 0.0001). The mechanism of action of NG52 may involve the inhibition of TGFβ1 expression and the activation of the signaling pathway. Consequently, targeting the PGK1 pathway holds promise as a therapeutic strategy to mitigate aortic neointimal hyperplasia.</p>","PeriodicalId":2,"journal":{"name":"ACS Applied Bio Materials","volume":" ","pages":""},"PeriodicalIF":4.6,"publicationDate":"2025-06-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144315481","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Nanohybrid Fibers via Direct Nanoparticle Injection into the Spider's Silk Gland.","authors":"Anastasia Kryuchkova, Mariia Mikhailova, Pavel Zelenovskii, Igor Bdikin, Andrei Kholkin, Elena F Krivoshapkina, Pavel V Krivoshapkin","doi":"10.1021/acsabm.5c00680","DOIUrl":"https://doi.org/10.1021/acsabm.5c00680","url":null,"abstract":"<p><p>Spider silk demonstrates an impressive balance of high strength and elasticity, which results from the hierarchical self-assembled structure of spider silk proteins during the fiber biosynthesis and spinning process. Enhancing the mechanical characteristics of spider silk fibers and imparting them with functional properties has garnered considerable attention. This challenge underscores the importance of developing strategies for modifying native spider silk. In this study, we introduce an approach to modify the structure and properties of spider silk fibers by injecting magnetite hydrosols directly into the spiders' silk glands. This results not only in the magnetic functionality of spider silk fibers but also in 82% increase in Young's compared to native spider silk, along with hardness of 1.30 MPa. To explore the nature of this phenomenon, we analyzed the difference in the topography of native <i>Holothele incei</i> spider silk and Fe₃O₄-hybrid spider silk, as well as their corresponding mechanical behavior at the nanoscale. Additionally, we studied the changes in structure, composition, and morphology caused by the inclusion of magnetic nanoparticles. Our findings demonstrate that the polar and hydrophobic interactions between Fe₃O₄ nanoparticles and the amino acid residues in spider silk could influence Young's modulus and hardness of the Fe₃O₄/spider silk hybrid fibers by promoting the protein conformation from an amorphous phase to β-sheets. This can only be achieved when nanomaterials are integrated into the structure within the fiber. The developed approach enables the fabrication of modified spider silk fibers, which can aid in the fundamental study of native spider silk and the development of technologies to fully replicate the properties of native silk in the future. Furthermore, lightweight, flexible, but strong materials are critical in soft robotic applications, where these nanohybrid fibers not only ensure gentle manipulation and reliability, but also their magnetic properties allow for responsive movement and control.</p>","PeriodicalId":2,"journal":{"name":"ACS Applied Bio Materials","volume":" ","pages":""},"PeriodicalIF":4.6,"publicationDate":"2025-06-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144315483","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"In Situ Electrochemical Fabrication of Photoreactive Ag-Cu Bimetallic Nanocomposite Coating and Its Antibacterial-Osteogenic Synergy.","authors":"Henigul Osman, Xiaohui Tang, Qin Wei, Bo Liu, Jie Gao, Yingbo Wang","doi":"10.1021/acsabm.5c00802","DOIUrl":"https://doi.org/10.1021/acsabm.5c00802","url":null,"abstract":"<p><p>In response to the issues of infection and poor bone integration in orthopedic implants, this study successfully developed a multifunctional composite coating composed of poly(pyrrole) (PPy), hydroxyapatite (HA), and silver-copper (Ag-Cu) bimetallic nanoparticles (NPs) on titanium (Ti) substrates using an electrochemical in situ deposition technique. Upon near-infrared light (NIR, 808 nm) stimulation, this coating exhibits synergistic antibacterial and osteogenic effects through photothermal (PTT) and photodynamic (PDT) processes. Characterization results indicate that the Ag-Cu NPs are uniformly distributed within the coating (Ag: 1.7 wt %, Cu: 2.0 wt %), and the concentrations of Ag⁺ and Cu²⁺ released (2.9526 mg·L^-1 and 0.1932 mg·L^-1, respectively) are significantly lower than the cytotoxic threshold (10 mg·L^-1). PTT tests reveal that the coating achieves a PTT conversion efficiency of 33.8%, with the temperature rising to 49.9 °C within 10 min under 1.0 W·cm^-2 irradiation, generating high levels of singlet oxygen (¹O₂). This leads to a 100% bactericidal rate against <i>Escherichia coli</i> and <i>Staphylococcus aureus</i>. In vitro biocompatibility assays show that the gradient release of HA and the synergistic effect of Ag⁺/Cu²⁺ significantly enhance the proliferation of bone marrow mesenchymal stem cells (BMSCs), with optical density reaching 1.49 after 7 days of culture. Additionally, osteogenic differentiation is promoted, as evidenced by a 2.9-fold increase in alkaline phosphatase (ALP) activity and a 2.1-fold increase in calcium nodule formation. Western blot analysis further confirmed that the coating induces the high expression of Runx2 via activation of the Wnt/β-catenin signaling pathway, thereby driving osteogenesis. This study presents a strategy for the development of smart implants with both efficient antibacterial and bone integration capabilities.</p>","PeriodicalId":2,"journal":{"name":"ACS Applied Bio Materials","volume":" ","pages":""},"PeriodicalIF":4.6,"publicationDate":"2025-06-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144315480","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}