Neha Dubey, Sonali Gupta, Sandeep B Shelar, Bijaideep Dutta, Kanhu C Barick, Sudeshna Chandra
{"title":"刺激响应上转换纳米颗粒-嵌入介孔SiO2纳米球用于传递亲疏水抗癌药物和细胞成像。","authors":"Neha Dubey, Sonali Gupta, Sandeep B Shelar, Bijaideep Dutta, Kanhu C Barick, Sudeshna Chandra","doi":"10.1021/acsabm.5c00966","DOIUrl":null,"url":null,"abstract":"<p><p>Core-shell architectures are widely reported for enhancing the luminescence properties of upconversion nanoparticles (UCNPs) by minimizing surface quenching and optimizing energy transfer pathways. However, this study demonstrates a hybrid structure in which UCNPs (CaF<sub>2</sub>:Yb<sup>3+</sup>, Er<sup>3+</sup>) are embedded in a mesoporous silica (<i>m</i>-SiO<sub>2</sub>) nanosphere using a soft chemical approach, offering an alternative to conventional core-shell structures. The structural analysis by X-ray diffraction (XRD) and transmission electron microscopy (TEM) confirmed the successful entrapment of highly crystalline cubic phase fluorite-type UCNPs in a mesoporous SiO<sub>2</sub> framework (UCNPs@m-SiO<sub>2</sub>). These UCNPs@m-SiO<sub>2</sub> exhibited a high surface area, porous nature, good colloidal stability, and pH-dependent charge-converting characteristics, which made them extremely conducive for drug delivery application. This matrix-based design not only stabilizes the nanoparticles but also significantly enhances their upconversion luminescence properties upon near-infrared (NIR) light irradiation (980 nm). Furthermore, the porous nature of the silica matrix allows efficient encapsulation of anticancer drugs, doxorubicin hydrochloride (DOX) and curcumin (CUR), and demonstrates their sustained and controlled pH-responsive release with higher release in a mildly acidic environment. The-drug loaded systems showed enhanced toxicity toward breast (MCF-7) and lung (A549) cancer cells over their individual counterparts (DOX and CUR). Moreover, the developed UCNPs@m-SiO<sub>2</sub> retained their red emission capability upon internalization into cancer cells and thus can also be used for cellular imaging purposes. Specifically, this work demonstrated the development of water-dispersible, biocompatible, and photostable UCNPs for image-guided drug delivery applications.</p>","PeriodicalId":2,"journal":{"name":"ACS Applied Bio Materials","volume":" ","pages":""},"PeriodicalIF":4.7000,"publicationDate":"2025-10-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Stimuli-Responsive Upconversion Nanoparticles-Embedded Mesoporous SiO<sub>2</sub> Nanospheres for Delivery of Hydrophilic and Hydrophobic Anticancer Drugs and Cellular Imaging.\",\"authors\":\"Neha Dubey, Sonali Gupta, Sandeep B Shelar, Bijaideep Dutta, Kanhu C Barick, Sudeshna Chandra\",\"doi\":\"10.1021/acsabm.5c00966\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><p>Core-shell architectures are widely reported for enhancing the luminescence properties of upconversion nanoparticles (UCNPs) by minimizing surface quenching and optimizing energy transfer pathways. However, this study demonstrates a hybrid structure in which UCNPs (CaF<sub>2</sub>:Yb<sup>3+</sup>, Er<sup>3+</sup>) are embedded in a mesoporous silica (<i>m</i>-SiO<sub>2</sub>) nanosphere using a soft chemical approach, offering an alternative to conventional core-shell structures. The structural analysis by X-ray diffraction (XRD) and transmission electron microscopy (TEM) confirmed the successful entrapment of highly crystalline cubic phase fluorite-type UCNPs in a mesoporous SiO<sub>2</sub> framework (UCNPs@m-SiO<sub>2</sub>). These UCNPs@m-SiO<sub>2</sub> exhibited a high surface area, porous nature, good colloidal stability, and pH-dependent charge-converting characteristics, which made them extremely conducive for drug delivery application. This matrix-based design not only stabilizes the nanoparticles but also significantly enhances their upconversion luminescence properties upon near-infrared (NIR) light irradiation (980 nm). Furthermore, the porous nature of the silica matrix allows efficient encapsulation of anticancer drugs, doxorubicin hydrochloride (DOX) and curcumin (CUR), and demonstrates their sustained and controlled pH-responsive release with higher release in a mildly acidic environment. The-drug loaded systems showed enhanced toxicity toward breast (MCF-7) and lung (A549) cancer cells over their individual counterparts (DOX and CUR). Moreover, the developed UCNPs@m-SiO<sub>2</sub> retained their red emission capability upon internalization into cancer cells and thus can also be used for cellular imaging purposes. Specifically, this work demonstrated the development of water-dispersible, biocompatible, and photostable UCNPs for image-guided drug delivery applications.</p>\",\"PeriodicalId\":2,\"journal\":{\"name\":\"ACS Applied Bio Materials\",\"volume\":\" \",\"pages\":\"\"},\"PeriodicalIF\":4.7000,\"publicationDate\":\"2025-10-03\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"ACS Applied Bio Materials\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://doi.org/10.1021/acsabm.5c00966\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q2\",\"JCRName\":\"MATERIALS SCIENCE, BIOMATERIALS\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"ACS Applied Bio Materials","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.1021/acsabm.5c00966","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"MATERIALS SCIENCE, BIOMATERIALS","Score":null,"Total":0}
Stimuli-Responsive Upconversion Nanoparticles-Embedded Mesoporous SiO2 Nanospheres for Delivery of Hydrophilic and Hydrophobic Anticancer Drugs and Cellular Imaging.
Core-shell architectures are widely reported for enhancing the luminescence properties of upconversion nanoparticles (UCNPs) by minimizing surface quenching and optimizing energy transfer pathways. However, this study demonstrates a hybrid structure in which UCNPs (CaF2:Yb3+, Er3+) are embedded in a mesoporous silica (m-SiO2) nanosphere using a soft chemical approach, offering an alternative to conventional core-shell structures. The structural analysis by X-ray diffraction (XRD) and transmission electron microscopy (TEM) confirmed the successful entrapment of highly crystalline cubic phase fluorite-type UCNPs in a mesoporous SiO2 framework (UCNPs@m-SiO2). These UCNPs@m-SiO2 exhibited a high surface area, porous nature, good colloidal stability, and pH-dependent charge-converting characteristics, which made them extremely conducive for drug delivery application. This matrix-based design not only stabilizes the nanoparticles but also significantly enhances their upconversion luminescence properties upon near-infrared (NIR) light irradiation (980 nm). Furthermore, the porous nature of the silica matrix allows efficient encapsulation of anticancer drugs, doxorubicin hydrochloride (DOX) and curcumin (CUR), and demonstrates their sustained and controlled pH-responsive release with higher release in a mildly acidic environment. The-drug loaded systems showed enhanced toxicity toward breast (MCF-7) and lung (A549) cancer cells over their individual counterparts (DOX and CUR). Moreover, the developed UCNPs@m-SiO2 retained their red emission capability upon internalization into cancer cells and thus can also be used for cellular imaging purposes. Specifically, this work demonstrated the development of water-dispersible, biocompatible, and photostable UCNPs for image-guided drug delivery applications.
期刊介绍:
ACS Applied Bio Materials is an interdisciplinary journal publishing original research covering all aspects of biomaterials and biointerfaces including and beyond the traditional biosensing, biomedical and therapeutic applications.
The journal is devoted to reports of new and original experimental and theoretical research of an applied nature that integrates knowledge in the areas of materials, engineering, physics, bioscience, and chemistry into important bio applications. The journal is specifically interested in work that addresses the relationship between structure and function and assesses the stability and degradation of materials under relevant environmental and biological conditions.