ACS Applied Bio Materials最新文献

{"title":"Dextran-Polydopamine Dual Coating on 3D-Printed Polycaprolactone Scaffolds as a Potential Biofunctionalization Platform for Bone Tissue Engineering","authors":"MinJoo Kim*,&nbsp;, ,&nbsp;Giles Michael Cheers,&nbsp;, ,&nbsp;Bastian Hartmann,&nbsp;, ,&nbsp;Hauke Clausen-Schaumann,&nbsp;, ,&nbsp;Aldo Roberto Boccaccini,&nbsp;, ,&nbsp;Boris Michael Holzapfel,&nbsp;, and ,&nbsp;Susanne Mayer-Wagner*,&nbsp;","doi":"10.1021/acsabm.5c01304","DOIUrl":"10.1021/acsabm.5c01304","url":null,"abstract":"<p >Three-dimensional (3D) printing has paved the way for the precision manufacturing of patient-specific scaffolds. While personalized 3D-printed bone scaffolds are already in the clinic, further attempts to combine biofunctionalization and drug delivery with these scaffolds are of great interest to improve tissue regeneration and reduce recovery time. This study investigated the dextran-polydopamine (PDA) dual-coated 3D-printed polycaprolactone (PCL) scaffolds as a potential biofunctionalization platform, which will enable the design of more advanced coating systems. Despite PCL being one of the most well-established biomaterials used in manufacturing bone scaffolds, surface modification is essential for its application due to its hydrophobic surface and lack of osteogenic properties. PDA is a bioinspired synthetic polymer, known for its convenient coating strategy, superior osteogenicity, and ability to graft secondary biofunctionalization motifs. However, modifying the surface of PCL with PDA results in aggregates of PDA nanoparticles rather than forming a homogeneous coating layer. Here, dextran was introduced as a dual coating deposited as a thin layer, which further assists cell adhesion and proliferation. Dextran is a biomedical macromolecule with a long history in medicine, which can be used as a drug delivery carrier in various forms, and the focus of this study was to investigate the intricate interplay between dextran and PDA as a dual coating applied to 3D-printed PCL scaffolds, via microstructural, topographical, chemical, and mechanical validation. A series of cell studies using osteoblast-like MG-63 cells was conducted, and it has been confirmed that dextran can be introduced to the PDA-modified PCL scaffold while maintaining the maximum scaffold and cell interaction. Consequently, the present results suggest that the dextran-PDA dual coating offers a promising biofunctionalization platform for designing more complex systems involving dextran-based drug delivery, aimed at application in bone tissue engineering.</p>","PeriodicalId":2,"journal":{"name":"ACS Applied Bio Materials","volume":"8 10","pages":"9145–9160"},"PeriodicalIF":4.7,"publicationDate":"2025-10-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145256958","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Composite Hydrogel Loaded with Ginsenoside Rc and Cuttlefish Ink Nanoparticles: Photothermal–Immunomodulatory Synergy for Accelerated Healing of Bacteria-Infected Wounds","authors":"Wan-Shuang Zhang,&nbsp;, ,&nbsp;Kun Feng,&nbsp;, ,&nbsp;Tao Wang,&nbsp;, ,&nbsp;Fei Wang,&nbsp;, ,&nbsp;Yanyu Ma*,&nbsp;, ,&nbsp;Quanchi Chen*,&nbsp;, and ,&nbsp;Zezhang Zhu*,&nbsp;","doi":"10.1021/acsabm.5c01484","DOIUrl":"10.1021/acsabm.5c01484","url":null,"abstract":"<p >The infected wound healing remains challenges in the clinic due to the synergistic pathological effects of persistent inflammation, dysregulated oxidative stress, and compromised neovascularization. Existing therapeutic strategies often fail to simultaneously address these interlinked challenges. To address this issue, the GelMA@Rc/CINPs composite hydrogel was developed that innovatively integrates the photothermal antibacterial property of cuttlefish ink nanoparticles with the mitochondrial-protective effect of Rc to establish a multipathway cotherapeutic system. Rapidly gelated by blue light cross-linking, its nanoparticles can effectively clear biofilms, while ginsenoside Rc synergistically regulates the inflammatory response, ultimately accelerating the healing of infected wounds. With excellent size distribution and biocompatibility, the GelMA@Rc/CINPs composite hydrogel demonstrates significant potential for clinical translation in managing bacterial infection-related wound healing.</p>","PeriodicalId":2,"journal":{"name":"ACS Applied Bio Materials","volume":"8 10","pages":"9365–9378"},"PeriodicalIF":4.7,"publicationDate":"2025-10-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145273118","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Breaking through Microbial Defenses─Organic Acid-Based Deep Eutectic Solvents as a Neoteric Strategy in Bacterial Biofilms, Persister, and Fungal Control","authors":"Tomasz Swebocki*,&nbsp;, ,&nbsp;Aleksandra M. Kocot,&nbsp;, ,&nbsp;Karolina Cieminska,&nbsp;, ,&nbsp;Clovis Bortolus,&nbsp;, ,&nbsp;Jérôme Muchembled,&nbsp;, ,&nbsp;Meroua S. Mechouche,&nbsp;, ,&nbsp;Justine Jacquin,&nbsp;, ,&nbsp;Kamel Haddadi,&nbsp;, ,&nbsp;Ali Siah,&nbsp;, ,&nbsp;Boualem Sendid,&nbsp;, ,&nbsp;Rabah Boukherroub*,&nbsp;, and ,&nbsp;Magdalena Plotka,&nbsp;","doi":"10.1021/acsabm.5c01159","DOIUrl":"10.1021/acsabm.5c01159","url":null,"abstract":"<p >This study explores the adaptation of organic acid-based deep eutectic solvents (OA-DESs) as effective antimicrobial agents. Having already demonstrated their efficacy against planktonic bacteria in our previous research, herein we investigate their impact on more complex microbial forms, including biofilms, persister cells, and fungi (both human pathogenic and phytopathogenic). Our experiments revealed that OA-DESs effectively eradicated methicillin-resistant <i>Staphylococcus aureus</i> (MRSA) and <i>Escherichia coli</i> in the biofilms, inducing significant morphological changes. A three-log-unit reduction was observed for most OA-DESs at concentrations below 1% (v/v), a remarkable achievement for this class of materials. Additionally, with only one exception, OA-DESs did not promote persister cells formation, underscoring their potential for complete eradication of biofilm-enveloped bacteria. In another part of our study, OA-DESs were compared to conventional DESs against <i>Candida albicans</i>, <i>Candida auris</i>, and <i>Aspergillus fumigatus</i>. Results showed that while individual DES components exhibited minimal activity, their combination effectively inhibited fungal growth and induced substantial morphological changes. Lastly, OA-DESs were tested against the phytopathogens <i>Zymoseptoria tritici</i> and <i>Venturia inaequalis</i>. Though their activity was less pronounced compared to pathogenic strains, most OA-DESs inhibited the growth of both fungi at the highest tested concentrations. Despite the broad scope of this investigation, we believe this work provides valuable insights into the potential of DESs as antimicrobial agents, offering a strong foundation for future research and innovation in this field.</p>","PeriodicalId":2,"journal":{"name":"ACS Applied Bio Materials","volume":"8 10","pages":"8980–8990"},"PeriodicalIF":4.7,"publicationDate":"2025-10-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145256900","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A Supramolecular Material for Selective Fluorescence-Based Detection of the Neuromuscular Blocker Rocuronium Bromide","authors":"Bindurani Padhan,&nbsp; and ,&nbsp;Prabhat K. Singh*,&nbsp;","doi":"10.1021/acsabm.5c01490","DOIUrl":"10.1021/acsabm.5c01490","url":null,"abstract":"<p >Monitoring Rocuronium Bromide (RcBr), a neuromuscular blocker widely used in anesthesia, is clinically important yet analytically underexplored. We report a fluorescence-based sensor for RcBr using Thioflavin-T (ThT) aggregates templated by γ-sulfated cyclodextrin (γ-SCD). This competitive displacement assay exploits the high binding affinity of Rocuronium for γ-SCD to displace Thioflavin-T from supramolecular aggregates, resulting in a fluorescence turn-off response. The sensor exhibits submicromolar sensitivity (LOD ∼ 0.2 ± 0.02 μM), rapid response (&lt;1 min), and high selectivity over biological interferents. Its functionality in diluted human serum demonstrates potential for possible clinical applications. This work presents a novel optical approach for RcBr sensing that may support future developments in neuromuscular blockade monitoring.</p>","PeriodicalId":2,"journal":{"name":"ACS Applied Bio Materials","volume":"8 10","pages":"9357–9364"},"PeriodicalIF":4.7,"publicationDate":"2025-10-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145248950","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Toward Developing an Antimelanoma Topical Gel from Coordination Polymers Derived from a Nitrile-Functionalized Terpyridine Ligand and Dicarboxylic Acids","authors":"Tanmay Chanda,&nbsp;, ,&nbsp;Subhajit Ghosh,&nbsp;, and ,&nbsp;Parthasarathi Dastidar*,&nbsp;","doi":"10.1021/acsabm.5c01536","DOIUrl":"10.1021/acsabm.5c01536","url":null,"abstract":"<p >With the aim of developing a vehicle-free drug delivery (VFDD) system, a structural rationale has been exploited to synthesize a series of metallogelators derived from coordination polymers constituted with a nitrile-functionalized terpyridyl ligand (L), various dicarboxylic acids (2-amino terephthalate (2-NH₂TA), 2,6-naphthalene dicarboxylate (2,6-NDA), and 1,4-phenylene dicarboxylate (1,4-PDA)), and transition metal salts (Zn(NO₃)₂·6H₂O, Co(NO₃)₂·6H₂O, Ni(NO₃)₂·6H₂O). The coordination polymers (CP1-CP5), thus synthesized, were fully characterized by single-crystal X-ray diffraction (SXRD). Metallogels (CPG1-CPG5) were obtained by mixing stoichiometric amounts of the reactants of the coordination polymers in DMSO/water (3:2), as revealed by their single-crystal structures. The metallogels were characterized by dynamic rheology and electron microscopy. Biological studies revealed that the anticancer activity of the metallogelator CPG1 against the melanoma cell line (B16–F10) was superior to CPG2, with CPG1 being noncytotoxic to noncancerous cell lines (E. Derm and HEK 293) within the operating concentration range. CPG1 also showed appreciable ability to inhibit cell migration of B16–F10 cells in a scratch assay. Mechanistic studies indicated the involvement of apoptosis with cell cycle arrest in the G0/G1 phase. Side population discrimination assay showed that CPG1 could also kill the side population (SP) cells of B16–F10─a trait known to reduce the chance of metastasis. Intriguingly, CPG1 was also successful in preventing the growth of tumor spheroids derived from B16–F10 cells, highlighting its ability to prevent cancerous tumor growth. Thus, the corresponding metallogel CPG1 can further be developed as a topical gel for treating skin cancer-like scenarios because the B16–F10 cell line is often referred to as a human skin cancer model.</p>","PeriodicalId":2,"journal":{"name":"ACS Applied Bio Materials","volume":"8 10","pages":"9424–9438"},"PeriodicalIF":4.7,"publicationDate":"2025-10-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145248938","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Real-Time Monitoring of the Cytotoxic Effect of Oxygen-Sensitive Fluorescent Poly(styrene-maleic anhydride) Nanoparticles Using Electrical-Substrate Impedance Sensing","authors":"Fernando Pesantez Torres,&nbsp;, ,&nbsp;Elijah C. Feret,&nbsp;, ,&nbsp;Yubing Xie,&nbsp;, and ,&nbsp;Susan T. Sharfstein*,&nbsp;","doi":"10.1021/acsabm.5c01443","DOIUrl":"10.1021/acsabm.5c01443","url":null,"abstract":"<p >Fluorescent poly(styrene-<i>co</i>-maleic anhydride) (SMA) nanoparticle (NP) oxygen sensors show strong potential for visualizing in situ oxygen gradients in biomedical research. To expand their applications, it is essential to understand their cellular interactions. Electrical cell–substrate impedance sensing (ECIS) enables real-time monitoring of cell behavior by measuring the impedance of monolayer cultures with an alternating current. In this study, we used ECIS to assess the cytotoxicity of SMA NP oxygen sensors and further examined their effects through endothelial barrier function analysis and microscopy. The sensors showed no cytotoxicity at any dose, confirming their biocompatibility. However, the NPs incorporated into the extracellular matrix and may disrupt barrier function. These findings support further use of SMA NP oxygen sensors in biomedical research and highlight ECIS as a valuable tool for evaluating nanoparticle biocompatibility when traditional optical assays are limited by optical interference.</p>","PeriodicalId":2,"journal":{"name":"ACS Applied Bio Materials","volume":"8 10","pages":"9322–9331"},"PeriodicalIF":4.7,"publicationDate":"2025-10-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://pubs.acs.org/doi/pdf/10.1021/acsabm.5c01443","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145256955","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Green Synthesis of Carbon Quantum Dots from Gardenia Jasminoides for the Selective Detection of Fe3+ Ions and Cell Imaging","authors":"Meng Sun*,&nbsp;, ,&nbsp;Junwen Cai,&nbsp;, ,&nbsp;Kangrui Yang,&nbsp;, ,&nbsp;Wanying Zhang,&nbsp;, ,&nbsp;Mengru Sun,&nbsp;, ,&nbsp;Hua Meng,&nbsp;, ,&nbsp;Shengming Wang,&nbsp;, and ,&nbsp;Benqiang Rao*,&nbsp;","doi":"10.1021/acsabm.5c01324","DOIUrl":"10.1021/acsabm.5c01324","url":null,"abstract":"<p >The selective and sensitive detection of Fe³⁺ is of significant importance in environmental monitoring and biomedical applications due to its vital yet potentially toxic roles in biological systems. Herein, we report a fluorescent sensing platform based on carbon quantum dots (CQDs) synthesized from <i>Gardenia jasminoides</i> via a facile, eco-friendly hydrothermal method. The as-synthesized CQDs feature an average particle size of 2.76 nm, strong blue fluorescence with an emission peak at 435 nm, and a moderate quantum yield of 13.7%. Notably, the CQDs exhibit excellent selectivity and sensitivity for Fe³⁺, along with robust stability over a broad pH range (2–10), under high ionic strength, in the presence of H₂O₂, and upon prolonged oxidative stress. Cytotoxicity assays reveal concentration-dependent behavior: high biosafety up to 500 μg/mL, yet significant antiproliferative activity against tumor cells above this threshold. Cellular imaging of A549 cells shows strong, dose-dependent cytoplasmic fluorescence at 1000 and 2000 μg/mL. These combined properties establish the <i>Gardenia</i>-derived CQDs as promising candidates for trace Fe³⁺ detection and high-contrast bioimaging.</p>","PeriodicalId":2,"journal":{"name":"ACS Applied Bio Materials","volume":"8 10","pages":"9245–9253"},"PeriodicalIF":4.7,"publicationDate":"2025-10-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145248955","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Development of Tunable Hard and Soft Lattice Scaffolds for Multiscale Tissue Engineering Applications","authors":"Jasmine Carpenter,&nbsp;, ,&nbsp;Elijah Barnes,&nbsp;, ,&nbsp;Amrita Natarajan,&nbsp;, ,&nbsp;Anjali Sudha,&nbsp;, ,&nbsp;Pratheesh V Kanakarajan,&nbsp;, ,&nbsp;Christopher J Panebianco,&nbsp;, ,&nbsp;Joel D Boerckel,&nbsp;, ,&nbsp;Derrick Dean,&nbsp;, and ,&nbsp;Vineeth M Vijayan*,&nbsp;","doi":"10.1021/acsabm.5c00818","DOIUrl":"10.1021/acsabm.5c00818","url":null,"abstract":"<p >The design of tunable hard and soft lattice scaffolds is key to advancing multiscale tissue engineering. In this study, we computationally designed and 3D-printed gyroid and diamond polylactic acid (PLA) scaffolds with varying lattice thicknesses and infills to modulate mechanical properties. Compression testing revealed a linear increase in modulus with increasing gyroid thickness (82–405 MPa), while diamond lattices with simple and body-centered infills reached up to 150 MPa, enabling tuning for both low- and high-density trabecular bone. Micro-CT analysis confirmed architectural fidelity, with scaffold porosity ranging from 63 to 85%, trabecular spacing (Tb.Sp) between 1.5 and 2.4 mm, and bone surface-to-volume ratios (BS/BV) of 3.2–6.4 mm²/mm³, suggesting tunability toward native trabecular bone. Surface modification with polydopamine (PDA) enhanced scaffold bioactivity, supporting robust human bone marrow-derived mesenchymal stem cell (hMSC) attachment, spreading, and stress fiber formation. Importantly, preliminary osteogenic evaluation revealed enhanced mineral deposition in PDA-coated scaffolds compared to uncoated PLA, with PDA-coated diamond architectures exhibiting the highest calcium deposition relative to both gyroid and uncoated diamond scaffolds. These results demonstrate that osteogenic potential can be tuned through both topology and surface modification. In parallel, soft scaffolds were developed by reinforcing alginate hydrogels with hydroxyapatite (HAP) nanocrystals and 3D bioprinting them into gyroid, hexagonal, and square honeycomb geometries. Rheological testing confirmed improved shear-thinning and print fidelity with increasing HAP content. Cell encapsulation studies with fibroblasts revealed scaffold-dependent differences, where Alamar Blue and PicoGreen assays demonstrated the highest metabolic activity and DNA content in the square honeycomb design, followed by hexagonal and gyroid lattices. Together, these findings establish a framework in which lattice geometry, material reinforcement, and surface biofunctionalization can be systematically combined to create tunable scaffolds for both load-bearing and soft tissue applications, laying the groundwork for hybrid systems with spatial and mechanical gradients to regenerate complex tissues.</p>","PeriodicalId":2,"journal":{"name":"ACS Applied Bio Materials","volume":"8 10","pages":"8735–8750"},"PeriodicalIF":4.7,"publicationDate":"2025-10-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145243265","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Graphitic Carbon Nitride-Reinforced Chitosan/Ca2+-Alginate Nanocomposite Films with Multifunctional Properties for Sustainable Packaging","authors":"Md Sajib Hossain,&nbsp;, ,&nbsp;Bapan Adak*,&nbsp;, ,&nbsp; Khushbu,&nbsp;, and ,&nbsp;Samrat Mukhopadhyay*,&nbsp;","doi":"10.1021/acsabm.5c01293","DOIUrl":"10.1021/acsabm.5c01293","url":null,"abstract":"<p >This study presents the synthesis and characterization of Ca²⁺-cross-linked alginate (ALG), chitosan (CHT), and g-C₃N₄-based ternary composites for multifunctional applications. The electrostatic interactions between ALG and CHT, combined with the ionic interaction of Ca²⁺ with ALG, significantly enhanced the composite properties. Furthermore, incorporation of g-C₃N₄ into the ALG/CHT matrix resulted in a uniform dispersion in the ALG/g-C₃N₄/CHT composite, as confirmed by morphological analysis, due to strong hydrogen bonding among the components. The resulting composite exhibited improved water vapor barrier, mechanical strength, and antimicrobial activity. Specifically, the ALG/g-C₃N₄/CHT composite (with g-C₃N₄ loading 1.33%) achieved a tensile strength of 88.4 MPa and a Young’s modulus of 1.6 GPa, representing increases of 19.4 and 100.6% over pure ALG and CHT films, respectively. The UV shielding performance of the ALG/g-C₃N₄/CHT composite film was exceptional, providing exceptional UV protection across the entire UV spectrum (100–400 nm), with blocking efficiencies of 97.9% (UV-A), 98.2% (UV-B), and 99.7% (UV-C). Additionally, water vapor permeability of the ALG/g-C₃N₄/CHT composite film decreased by 79 and 92% compared to those of CHT and ALG films. The composite also demonstrated high antibacterial activity against <i>Staphylococcus aureus</i> (97.7%) and <i>Escherichia coli</i> (98.2%), along with a radical scavenging activity of 61%. These outstanding multifunctional properties highlight the potential of this ternary composite as a sustainable alternative to conventional petroleum-based packaging materials.</p>","PeriodicalId":2,"journal":{"name":"ACS Applied Bio Materials","volume":"8 10","pages":"9123–9139"},"PeriodicalIF":4.7,"publicationDate":"2025-10-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145243346","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Topical Nanocarrier-Assisted Corticosteroid Delivery Combined with Phototherapy for Effective Atopic Dermatitis Treatment","authors":"Young-Ae Choi,&nbsp;, ,&nbsp;Jun Young Park,&nbsp;, ,&nbsp;Jinjoo Kang,&nbsp;, ,&nbsp;Na-Hee Jeong,&nbsp;, ,&nbsp;Soyoung Lee,&nbsp;, ,&nbsp;Dongwoo Khang*,&nbsp;, and ,&nbsp;Sang-Hyun Kim*,&nbsp;","doi":"10.1021/acsabm.5c01594","DOIUrl":"10.1021/acsabm.5c01594","url":null,"abstract":"<p >Topical corticosteroids are widely used to manage chronic inflammatory skin diseases, such as atopic dermatitis (AD). However, their limited skin penetration necessitates high doses and prolonged use, which increases the risk of local and systemic side effects. To address these limitations, we developed a gold nanorod–conjugated dexamethasone (AuNR-DEX) system combined with red light-emitting diode (LED) irradiation to enhance transdermal drug delivery and therapeutic efficacy. AuNR conjugation enhanced the physicochemical properties of DEX, including its hydrodynamic diameter and surface charge, thereby facilitating increased skin penetration. In an AD mouse model, topical AuNR-DEX significantly alleviated disease symptoms at only 20% of the dose required for free DEX. While AuNR-DEX alone showed limited dispersion across the skin, cotreatment with red LED irradiation facilitated broader spreading and improved drug distribution. The combination therapy markedly restored epidermal differentiation, collagen fiber integrity, and calcium homeostasis in AD skin lesions. In vitro, AuNR-DEX plus LED treatment enhanced keratinocyte survival, restored the localization of tight junction proteins, and reduced mitochondrial calcium overload and reactive oxygen species levels. Overall, the synergistic effect of AuNR-DEX and red LED irradiation enhanced drug delivery, targeted dispersion, and therapeutic outcomes, offering a promising strategy for treating AD with reduced corticosteroid dosage and minimized side effects.</p>","PeriodicalId":2,"journal":{"name":"ACS Applied Bio Materials","volume":"8 10","pages":"9464–9479"},"PeriodicalIF":4.7,"publicationDate":"2025-10-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145237502","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}