Digital Light Processing of Methacrylated Silk Fibroin Microneedles: Precision Engineering for Enhanced Transdermal Delivery and Skin Regeneration.

IF 4.6 Q2 MATERIALS SCIENCE, BIOMATERIALS

ACS Applied Bio Materials Pub Date : 2025-07-21 Epub Date: 2025-07-07 DOI:10.1021/acsabm.5c00621

Fangzheng Tong, Xuyang Zhang, Xuanwen Wang, Siying Liu, Xiaoliang Cui, Yajie Zhou, Gang Li, Jun Zhang

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引用次数: 0

Abstract

Despite the promising potential of digital light processing (DLP)─fabricated silk fibroin (SF) microneedles (MNs) in transdermal applications, their clinical translation faces two major challenges: insufficient manufacturing precision to achieve architectural resolution and inadequate mechanical strength to penetrate the epidermis effectively. To overcome these critical barriers, we developed a methacrylated silk fibroin (Sil-MA) bioink that combines high biocompatibility with enhanced printability. By systematically optimizing material formulation, printing parameters and MN geometry, we successfully fabricated DLP-printed Sil-MA MNs with precise architecture control and superior mechanical performance. These MNs demonstrated no cytotoxicity and enabled efficient transdermal delivery of three distinct dermatological therapeutics. Comprehensive in vitro and in vivo assessments indicated that the DLP-printed Sil-MA MNs also acted as mechanical stimulators, significantly promoting epidermal keratinocyte proliferation. This engineered platform offers a versatile strategy for developing multifunctional MN systems for therapeutic delivery and tissue engineering applications.

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甲基丙烯酸丝素微针的数字光处理：增强透皮传递和皮肤再生的精密工程。

尽管数字光处理（DLP）制造的丝素蛋白（SF）微针（MNs）在透皮应用中有很大的潜力，但它们的临床转化面临两个主要挑战：制造精度不足，无法实现结构分辨率，机械强度不足，无法有效穿透表皮。为了克服这些关键障碍，我们开发了一种甲基丙烯酸丝素（Sil-MA）生物链接，它结合了高生物相容性和增强的印刷性。通过系统地优化材料配方、打印参数和MN的几何形状，我们成功地制造了具有精确结构控制和优异机械性能的dlp打印Sil-MA MN。这些MNs显示没有细胞毒性，并且能够有效地经皮递送三种不同的皮肤疗法。体外和体内综合评价表明，dlp打印的Sil-MA MNs还具有机械刺激作用，显著促进表皮角质细胞增殖。该工程平台为开发用于治疗递送和组织工程应用的多功能MN系统提供了一种通用策略。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

ACS Applied Bio Materials Chemistry-Chemistry (all)

CiteScore

9.40

自引率

2.10%

发文量

464

期刊介绍： ACS Applied Bio Materials is an interdisciplinary journal publishing original research covering all aspects of biomaterials and biointerfaces including and beyond the traditional biosensing, biomedical and therapeutic applications. The journal is devoted to reports of new and original experimental and theoretical research of an applied nature that integrates knowledge in the areas of materials, engineering, physics, bioscience, and chemistry into important bio applications. The journal is specifically interested in work that addresses the relationship between structure and function and assesses the stability and degradation of materials under relevant environmental and biological conditions.