Animal Reproduction最新文献

Effects of maternal temperament on uterine and umbilical blood flow, expression of angiogenic proteins, and fetal growth in pregnant ossimi sheep. 母性气质对妊娠奥西米羊子宫和脐血流量、血管生成蛋白表达及胎儿生长的影响。

IF 1.6 4区农林科学

Animal Reproduction Pub Date : 2025-07-07 eCollection Date: 2025-01-01 DOI: 10.1590/1984-3143-AR2024-0125

Mohammed Elmetwally, Fatma Adlan, Basma Hendam, Alaa Samy, Shaymaa Rezk, Samah Lasheen, Heba Mohram, Sara Shalapy, Reham Fahmy, Amy Jo Montgomery, Yasser Y Lenis, Amira Mostagir

{"title":"Effects of maternal temperament on uterine and umbilical blood flow, expression of angiogenic proteins, and fetal growth in pregnant ossimi sheep.","authors":"Mohammed Elmetwally, Fatma Adlan, Basma Hendam, Alaa Samy, Shaymaa Rezk, Samah Lasheen, Heba Mohram, Sara Shalapy, Reham Fahmy, Amy Jo Montgomery, Yasser Y Lenis, Amira Mostagir","doi":"10.1590/1984-3143-AR2024-0125","DOIUrl":"10.1590/1984-3143-AR2024-0125","url":null,"abstract":"This study aimed to examine the efficacy of Doppler and B-mode sonography in evaluating the impact of maternal temperament on hemodynamic alterations in both the fetus and uterus during ovine gestation in addition to alteration of the angiogenic protein expression and fetal biometry. Twenty Ossimi ewes were divided into two groups, more reactive (MR: 12 sheep) and less reactive (LR: 8 sheep). Several endpoints were assessed every 2 weeks (wk) from breeding to wk 20 of pregnancy. Blood samples were taken to evaluate the expression of angiogenic proteins at parturition. The resistance (RI) and pulsatility (PI) indexes of the uterine (UtA) and fetal umbilical arteries (UMA) were measured. Biometry includes: diameter of amniotic vesicles (AVD), umbilicus (UMD), fetal thoracic diameter (FTD) and metacarpal length (MCL). The UtA-PI was higher in MR compared to LR ewes between 6-12 wks of gestation (P>0.01), while a tendency was recognized at wk 14 (P=0.054). The same was true for UtA-RI during the first 8 wk of pregnancy (P < 0.03) when MR and LR animals were compared. Similarly, UMA-RI was higher in fetuses of MR than LR ewes at wk 14 (P<0.0003) and 20 (P<0.02) of pregnancy. The differences in UMA-PI reached significance at wk 6, 8, 10, and 20 (P<0.0 -0.003). Furthermore, significant changes in fetal biometry were investigated. The expressions of VEGF, NOS3 and HIF 1α were increased in the less reactive sheep (P<0.001). In conclusion, the maternal temperaments affect the Doppler, B mode as well as the expressions of mRNAs for VEGF, NOS3 and HIF 1α genes at time of parturition.","PeriodicalId":7889,"journal":{"name":"Animal Reproduction","volume":"22 2","pages":"e20240125"},"PeriodicalIF":1.6,"publicationDate":"2025-07-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12232499/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144582850","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Reproduction in the frog Aubria subsigillata (Dumeril 1856): hormonal injection and captive breeding technique. 小野蛙的繁殖（Dumeril 1856）：激素注射和圈养繁殖技术。

IF 1.6 4区农林科学

Animal Reproduction Pub Date : 2025-07-07 eCollection Date: 2025-01-01 DOI: 10.1590/1984-3143-AR2024-0129

Houénafa Aimé Chrysostome Gansa, Hyppolite Agadjihouèdé

{"title":"Reproduction in the frog Aubria subsigillata (Dumeril 1856): hormonal injection and captive breeding technique.","authors":"Houénafa Aimé Chrysostome Gansa, Hyppolite Agadjihouèdé","doi":"10.1590/1984-3143-AR2024-0129","DOIUrl":"10.1590/1984-3143-AR2024-0129","url":null,"abstract":"Aubria subsigillata (Dumeril 1856) is a frog under increasing threat that is intensely harvested by Beninese people. The aim of this study was to develop a captive breeding program to repopulate the natural habitat of frogs in Benin Republic in view to conserve anuran biodiversity. The methodology adopted consisted of combining broodstock not injected with ovulin (hCG) in netted cages (natural reproduction) and broodstock injected with ovulin in tanks (assisted reproduction). In vitro fertilization of unfertilized female eggs by male milt was also carried out (controlled reproduction). In natural reproduction, A. subsigillata eggs or tadpoles are not produced. The concentration of 0.2 IU/g administered by intra-femoral injection resulted in the release of gametes in both sexes. Assisted reproduction enabled eggs to be obtained from the three coupling tanks after a post-injection lag time varying between 93 and 94 hours (Tank one = 304 eggs; Tank two = 125 eggs; Tank three = 56 eggs) and at a water temperature of 26°C; however, there was no incubation of eggs. For controlled reproduction, the average time between injection and first spermiation was 13 hours for males and 27 hours for females post-injection at a temperature of 28.5°C. Fecundity varied between 56 and 329 eggs. The eggs had an average weight of 1 mg and were incubated between 164 and 168 hours after fertilization. However, the incubation rate decreased on the 5th day due to infection of the eggs by Saprolegnia sp. These results suggest that the experiment is partially viable: while hormonal stimulation can induce spawning and fertilization under controlled conditions, the success of incubation and development remains limited, highlighting the need for improvements in biosecurity and egg management to ensure full viability of captive breeding efforts.","PeriodicalId":7889,"journal":{"name":"Animal Reproduction","volume":"22 2","pages":"e20240129"},"PeriodicalIF":1.6,"publicationDate":"2025-07-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12232497/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144582852","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Quantification of polyoocyte follicles (POFs) in domestic cats. 家猫多卵母细胞卵泡（POFs）的定量测定。

IF 1.6 4区农林科学

Animal Reproduction Pub Date : 2025-07-04 eCollection Date: 2025-01-01 DOI: 10.1590/1984-3143-AR2024-0127

Alexandra Lays Petri, Mariana Valentini Casagrande, Adalgiza Pinto, Jonatas Cattelam, Camila Keterine Gorzelanski Trenkel, Jacqueline Zanella, Leonardo Gruchouskei

{"title":"Quantification of polyoocyte follicles (POFs) in domestic cats.","authors":"Alexandra Lays Petri, Mariana Valentini Casagrande, Adalgiza Pinto, Jonatas Cattelam, Camila Keterine Gorzelanski Trenkel, Jacqueline Zanella, Leonardo Gruchouskei","doi":"10.1590/1984-3143-AR2024-0127","DOIUrl":"10.1590/1984-3143-AR2024-0127","url":null,"abstract":"Ovarian follicles usually involve only one oocyte. However, the existence of follicles with more than one oocyte has been described in different species. Therefore, this study evaluated the occurrence and number of Polyoocyte Follicles (POFs) in the ovaries of cats undergoing the ovariohysterectomy technique. For this purpose, ovaries from 33 cats were collected and submitted to histological preparation and analysis. The slides were evaluated under a microscope by two evaluators, who quantified the presence of follicles containing one or more oocytes and classified the follicular stage. Monoocyte follicles (MOFs) were found in all ovaries. POFs were observed at similar frequencies in both the right and left ovaries. The frequency of POFs with two oocytes was 79.03% in the right ovaries and 75.75% in the left ovaries. Follicles with three oocytes were found in 16.12% of the right ovaries and 27.27% of the left ovaries. The mean number of follicles with zero, one, two, three or more oocytes was similar between the ovaries, being 6.58, 310.5, 3.25, and 0.29 in the right ovary and 6.25, 312.7, 4.25, and 0.41 in the left ovary (p-values of 0.7569, 0.9654, 0.4785, and 0.5015, respectively). POFs containing from two to five oocytes were identified in different stages of development. POFs at all stages of development were found in both ovaries, and the occurrence of these structures was influenced by the age, number of pregnancies, number of estrus, and weight of the cats studied.","PeriodicalId":7889,"journal":{"name":"Animal Reproduction","volume":"22 2","pages":"e20240127"},"PeriodicalIF":1.6,"publicationDate":"2025-07-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12232498/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144582851","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Effect of dietary supplementation of spray-dried plasma on performance and semen quality in aging broiler breeders. 饲粮中添加喷雾干燥血浆对老龄肉种鸡生产性能和精液质量的影响。

IF 1.6 4区农林科学

Animal Reproduction Pub Date : 2025-06-30 eCollection Date: 2025-01-01 DOI: 10.1590/1984-3143-AR2024-0111

Carlos Alexandre Granghelli, Mário Henrique Scapin Lopes, Luís Rangel, Joy Campbell, Javier Polo, Joe Crenshaw, Eneiva Carla Carvalho Celeghini, Pedro Nacib Jorge-Neto, Helena Lage Ferreira, Vinicius Santos Moura, Jennifer Soutter Motta, Cristiane Soares da Silva Araujo, Lucio Francelino Araujo

{"title":"Effect of dietary supplementation of spray-dried plasma on performance and semen quality in aging broiler breeders.","authors":"Carlos Alexandre Granghelli, Mário Henrique Scapin Lopes, Luís Rangel, Joy Campbell, Javier Polo, Joe Crenshaw, Eneiva Carla Carvalho Celeghini, Pedro Nacib Jorge-Neto, Helena Lage Ferreira, Vinicius Santos Moura, Jennifer Soutter Motta, Cristiane Soares da Silva Araujo, Lucio Francelino Araujo","doi":"10.1590/1984-3143-AR2024-0111","DOIUrl":"10.1590/1984-3143-AR2024-0111","url":null,"abstract":"A total of 216 Cobb 500 broiler breeder hens were randomly distributed across two treatments consisting of diets supplemented with 0 or 1% spray-dried plasma (SDP), resulting in 27 replications per treatment and four birds per pen. In addition, 36 roosters, housed in individual pens, were allocated to the same treatments, with each bird considered a replicate, in order to assess the influence of SDP on semen and hatching characteristics. The experimental diets were fed from 26 weeks until the conclusion of the study, at 65 weeks of age. Semen quality, embryonic mortality and quality of newly hatched chicks were evaluated at 29, 45, and 63 weeks. Hens were inseminated with fresh semen, and eggs were incubated following standard procedures. Semen from 63-week-old roosters had higher sperm concentration than other age groups, while 29-week-old rooster semen exhibited higher progressive motility than semen from older roosters (P < 0.001). The 45-week-old roosters had the lowest values for the analyzed semen quality parameters (average path velocity, straight-line velocity and curvilinear velocity). Additionally, sperm from 45-week-old roosters, regardless of SDP supplementation, had fewer total morphological defects than 63-week-old roosters. However, 1% SDP in the diet reduced total sperm cell defects at 63 weeks of age, as well as total sperm head and tail defects (P < 0.05) compared to unsupplemented birds. Dietary SDP reduced late embryonic death in 63-week-old breeders (P < 0.05). The results demonstrate that 1% SDP in breeder diets reduced late embryonic mortality and sperm cell defects, mainly in aged birds, enhancing the reproductive performance of broiler breeders.","PeriodicalId":7889,"journal":{"name":"Animal Reproduction","volume":"22 2","pages":"e20240111"},"PeriodicalIF":1.6,"publicationDate":"2025-06-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12212462/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144537805","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Effects of cactus (Opuntia ficus-indica) mucilage on boar sperm cryosurvival. 仙人掌（Opuntia ficus-indica）粘液对野猪精子低温存活的影响。

IF 1.6 4区农林科学

Animal Reproduction Pub Date : 2025-06-30 eCollection Date: 2025-01-01 DOI: 10.1590/1984-3143-AR2024-0004

Juan Antonio Ramírez-Chequer, Marco Antonio Lopez-Carlos, Carlos Fernando Arechiga-Flores, Pedro Hernandez-Briano, Carlos Aurelio Medina-Flores, Fabiola Mendez-Llorente

{"title":"Effects of cactus (Opuntia ficus-indica) mucilage on boar sperm cryosurvival.","authors":"Juan Antonio Ramírez-Chequer, Marco Antonio Lopez-Carlos, Carlos Fernando Arechiga-Flores, Pedro Hernandez-Briano, Carlos Aurelio Medina-Flores, Fabiola Mendez-Llorente","doi":"10.1590/1984-3143-AR2024-0004","DOIUrl":"10.1590/1984-3143-AR2024-0004","url":null,"abstract":"This study aimed to evaluate the effect of three inclusion levels of Opuntia ficus-indica mucilage (OFM) to the lactose-egg yolk extender on the quality of frozen-thawed boar semen. Semen samples (n=22) were split into four OFM treatments: 0.0 (control), 3.3, 6.7, or 10.0% OFM. The semen was packaged in 0.5 mL straws and frozen using conventional methods. The sperm motility, viability, membrane integrity (HOST), and morphology (intact acrosome and normal sperm) characteristics were evaluated immediately post-thaw. Additionally, sperm were incubated at 37 ºC during 3.5 h and monitored hourly in a termoresistance test (TRT) to assess sperm motility, viability, and membrane integrity, The study showed that adding OFM to the freezing extender significantly improved (P < 0.05) sperm quality characteristics, without affecting (P > 0.05) acrosome integrity or sperm abnormalities. Furthermore, the OFM addition improved (P < 0.05) the sperm viability and membrane integrity for up to 3.5 h of incubation. Results indicate that OFM can be added to the extender at a 6.7% OFM inclusion level to improve the quality of cryopreserved boar sperm.","PeriodicalId":7889,"journal":{"name":"Animal Reproduction","volume":"22 2","pages":"e20240004"},"PeriodicalIF":1.6,"publicationDate":"2025-06-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12212463/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144537806","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Melatonin mitigates PGF-induced apoptosis during luteal regression in heat-exposed rats. 褪黑素减轻热暴露大鼠黄体退化过程中pgf诱导的细胞凋亡。

IF 1.6 4区农林科学

Animal Reproduction Pub Date : 2025-06-30 eCollection Date: 2025-01-01 DOI: 10.1590/1984-3143-AR2024-0122

Hadi Tavakolikazerooni, Hao Yu, Saif Ullah, Wael Ennab, Dagan Mao

{"title":"Melatonin mitigates PGF-induced apoptosis during luteal regression in heat-exposed rats.","authors":"Hadi Tavakolikazerooni, Hao Yu, Saif Ullah, Wael Ennab, Dagan Mao","doi":"10.1590/1984-3143-AR2024-0122","DOIUrl":"10.1590/1984-3143-AR2024-0122","url":null,"abstract":"This study investigates the protective effects of melatonin against heat exposure during PGF-induced luteal regression in rats. Seventy-five PMSG and hCG primed rats were divided into three groups: non-heat-exposure (NHE), heat-exposure (HE), and melatonin plus heat-exposure (M+HE). The HE group underwent daily heat exposure (41°C for 2 h) for 7 days, while the M+HE group received intraperitoneal injection of melatonin (10 mg/kg body weight) before each heat session. On Day 7, PGF was administered, and ovarian samples were collected at 0, 1, 2, 8, and 24 h post-PGF. One set of ovaries was processed for histological analysis, including H&E staining, immunohistochemistry, and transmission electron microscopy and the other set was processed for Western blot for apoptotic protein expression. Results showed that heat exposure increased ovarian weight, disrupted follicular development, and elevated ovarian apoptotic markers (Caspase-3 and Bax), leading to luteal cell damage. Melatonin preserved ovarian weight, improved follicular and luteal structure, reduced atretic follicles, and mitigated luteal cell degeneration. In addition, melatonin decreased apoptotic marker expression and the Bax/Bcl-2 ratio, particularly at 16 and 24 h. These findings suggest melatonin protects luteal cells from heat-induced apoptosis during PGF-triggered regression, supporting reproductive function.","PeriodicalId":7889,"journal":{"name":"Animal Reproduction","volume":"22 2","pages":"e20240122"},"PeriodicalIF":1.6,"publicationDate":"2025-06-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12212464/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144537807","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

An integrated analysis of second- and third-generation transcriptome sequencing technologies reveals the DAZAP1 function in pig testis. 对第二代和第三代转录组测序技术的综合分析揭示了DAZAP1在猪睾丸中的功能。

IF 1.6 4区农林科学

Animal Reproduction Pub Date : 2025-06-27 eCollection Date: 2025-01-01 DOI: 10.1590/1984-3143-AR2024-012

Xia Zhang, Hailong Huo, Honglin Li, Yongqing Liu, Fujie Qiao, Changyao Li, Jinlong Huo

{"title":"An integrated analysis of second- and third-generation transcriptome sequencing technologies reveals the DAZAP1 function in pig testis.","authors":"Xia Zhang, Hailong Huo, Honglin Li, Yongqing Liu, Fujie Qiao, Changyao Li, Jinlong Huo","doi":"10.1590/1984-3143-AR2024-012","DOIUrl":"10.1590/1984-3143-AR2024-012","url":null,"abstract":"The quality of pig sperm is one of the crucial determinants of reproductive ability, and sperm defects can shorten the reproductive life of boars and affect the production of offspring. During transcription and translation, the DAZAP1 gene exerts regulatory control over alternative splicing, thereby exerting influence on vital cellular processes including cell growth, development, and spermatogenesis. In this study, we employed second- and third-generation transcriptome sequencing techniques to isolate and identify the DAZAP1 gene and its transcripts using Banna mini-pig inbred line (BMI) testicular cDNA as a template. We identified three splice variants of the DAZAP1 gene, including ENSSSCT00000023438.4 (DAZAP1_X1), ENSSSCT00000051975.3 (DAZAP1_X2), and ENSSSCT00000074738.2 (DAZAP1_X3). Furthermore, the transcript DAZAP1_X2, was subjected to comprehensive analysis. The DAZAP1_X2 variant comprises 13 exons, with a coding sequence (CDS) length of 1254 bp (417 aa). Subsequently, enrichment analyses based on GO and KEGG pathways revealed that DAZAP1_X2 primarily participated in pathways associated with spermatogenesis, movement of the 9+2 cilium structure, germ cell development, gamete generation, and sexual reproduction. The ceRNA analysis identified three miRNAs interacting with DAZAP1_X2: ssc-miR-107, ssc-miR-127, and ssc-miR-1343, which were primarily linked to spermatogenesis. Both the testis and urethral bulb had significant levels of DAZAP1 expression, according to multi-tissue expression analysis. Subcellular localization indicated that the DAZAP1 was mainly distributed in the cell nucleus. DAZAP1 was critical for sperm formation and was essential for reproductive. These results shed light on the biological roles of DAZAP1 in pigs.","PeriodicalId":7889,"journal":{"name":"Animal Reproduction","volume":"22 2","pages":"e20240141"},"PeriodicalIF":1.6,"publicationDate":"2025-06-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12203886/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144526143","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

β-hydroxybutyrate induces endoplasmic reticulum stress in bovine cumulus cells during oocyte maturation in vitro. β-羟基丁酸诱导牛卵丘细胞体外成熟过程中的内质网应激。

IF 1.6 4区农林科学

Animal Reproduction Pub Date : 2025-05-30 eCollection Date: 2025-01-01 DOI: 10.1590/1984-3143-AR2024-0106

Daniele Missio, Julia Koch, Zigomar da Silva, Francielli Weber Santos Cibin, Marcos Henrique Barreta, Vitor Braga Rissi, André Lucio Fontana Goetten, Fernando Silveira Mesquita, Valério Marques Portela, Paulo Bayard Dias Gonçalves, Rogério Ferreira

{"title":"β-hydroxybutyrate induces endoplasmic reticulum stress in bovine cumulus cells during oocyte maturation in vitro.","authors":"Daniele Missio, Julia Koch, Zigomar da Silva, Francielli Weber Santos Cibin, Marcos Henrique Barreta, Vitor Braga Rissi, André Lucio Fontana Goetten, Fernando Silveira Mesquita, Valério Marques Portela, Paulo Bayard Dias Gonçalves, Rogério Ferreira","doi":"10.1590/1984-3143-AR2024-0106","DOIUrl":"10.1590/1984-3143-AR2024-0106","url":null,"abstract":"Ketosis is a significant metabolic disorder caused by negative energy balance (NEB). β-hydroxybutyrate (BHBA) is the most abundant circulating ketone body and is known to accumulate within follicular fluid. Although NEB and metabolic stress conditions have been associated with reduced fertility in cows, the effect of increased BHBA on the viability and functionality of bovine cumulus-oocyte complexes (COC) is poorly understood. The aim of this study was to determine the effects of BHBA on expansion, oxidative status, and endoplasmic reticulum (ER) stress in cumulus cells, oocyte nuclear maturation, and in vitro embryo production (IVP) in cattle. Cumulus-oocyte complexes were treated with 0, 2, or 4 mM of BHBA for 6, 12, 18, or 24 h during oocyte in vitro maturation (IVM.) Cumulus expansion and mRNA levels of genes related to cumulus expansion, oxidative status, ER stress, and autophagy were evaluated. The oocytes were fixed for nuclear maturation analysis at 24 h of IVM. In addition, after IVM with BHBA for 24 hours, cleavage and blastocyst rates were examined. No difference was observed in the rates of oocytes reaching metaphase II, cleavage, and blastocysts. Furthermore, the oxidative status and relative abundance of mRNA for genes associated with antioxidant enzymes and autophagy were not altered by BHBA in cumulus cells. However, BHBA altered the total area of the COC and increased the mRNA levels of genes associated with ER. In conclusion, BHBA affects the expansion and induces ER stress in bovine cumulus cells during IVM without compromising oocyte nuclear maturation, oxidative status, cleavage, and blastocyst rates.","PeriodicalId":7889,"journal":{"name":"Animal Reproduction","volume":"22 2","pages":"e20240106"},"PeriodicalIF":1.6,"publicationDate":"2025-05-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12140510/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144233008","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Effects of the modified "Yijing Fang" on semen quality and reproductive hormones in male yaks with kidney yang deficiency: a focus on mitochondrial apoptosis pathways. 益精方对肾阳虚雄性牦牛精液质量及生殖激素的影响——以线粒体凋亡途径为重点

IF 1.6 4区农林科学

Animal Reproduction Pub Date : 2025-05-26 eCollection Date: 2025-01-01 DOI: 10.1590/1984-3143-AR2024-0139

Panfeng Chang, Hua Wang

引用次数: 0

Double artificial insemination in sheep: a comprehensive review. 绵羊双人工授精技术综述。

IF 1.6 4区农林科学

Animal Reproduction Pub Date : 2025-05-19 eCollection Date: 2025-01-01 DOI: 10.1590/1984-3143-AR2024-0055

Gabriel Maggi, Fabiane Pereira de Moraes, Fernando Caetano de Oliveira, Sergio Farias Vargas, Arnaldo Diniz Vieira, Rafael Gianella Mondadori, Bernardo Garziera Gasperin

{"title":"Double artificial insemination in sheep: a comprehensive review.","authors":"Gabriel Maggi, Fabiane Pereira de Moraes, Fernando Caetano de Oliveira, Sergio Farias Vargas, Arnaldo Diniz Vieira, Rafael Gianella Mondadori, Bernardo Garziera Gasperin","doi":"10.1590/1984-3143-AR2024-0055","DOIUrl":"10.1590/1984-3143-AR2024-0055","url":null,"abstract":"Artificial insemination (AI) in sheep presents variable results, especially when combined with estrus induction treatments during the anestrous period. Alternatives for obtaining better results without significantly altering the costs of hormonal protocols are essential because of the importance of this biotechnology in production systems. One alternative that potentially meets these requirements is double-AI. Therefore, this article aims to review the literature on double-cervical AI in sheep and identify gaps in existing knowledge. Double cervical superficial (CS) AI with frozen-thawed (F.T.) semen after estrus detection significantly increased pregnancy rates (PR) in most (6/8) evaluated studies, with an increase of 7 to 34.2 percentual points (p.p.), compared to single AI. Regarding fixed-time AI (FTAI), all studies used fresh (F) or chilled (C) semen, and no positive effects were observed for double FTAI in most cases (8/9). Most studies have not applied current estrous synchronization protocols and insemination doses. Therefore, further studies are needed to evaluate the potential benefits of double FTAI, especially using F.T. semen in combination with hormonal protocols and insemination doses aligned with current practices.","PeriodicalId":7889,"journal":{"name":"Animal Reproduction","volume":"22 2","pages":"e20240055"},"PeriodicalIF":1.6,"publicationDate":"2025-05-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12091998/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144109336","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0