Animal Reproduction最新文献_第2页

Advances in ovarian follicle culture systems: exploring the interplay between cells, matrix, and ovarian architecture. 卵巢卵泡培养系统的进展：探索细胞、基质和卵巢结构之间的相互作用。

IF 2.1 4区农林科学

Animal Reproduction Pub Date : 2025-08-14 eCollection Date: 2025-01-01 DOI: 10.1590/1984-3143-AR2025-0066

Alberto Maria Luciano, Noemi Monferini, Ludovica Donadini, Pritha Dey, Fernanda Fagali Franchi, Valentina Lodde, Federica Franciosi

引用次数: 0

Deciphering embryo-maternal cross-talk: advanced in vitro approaches. 破译胚胎-母体串扰：先进的体外方法。

IF 2.1 4区农林科学

Animal Reproduction Pub Date : 2025-08-13 eCollection Date: 2025-01-01 DOI: 10.1590/1984-3143-AR2025-0059

Gislaine Dos Santos, María Gemma Millán de la Blanca, Yulia Nathaly Cajas, Rosane Mazzarella, Karina Cañón-Beltrán, Maria Encina Gonzalez Martínez, Dimitrios Rizos

{"title":"Deciphering embryo-maternal cross-talk: advanced in vitro approaches.","authors":"Gislaine Dos Santos, María Gemma Millán de la Blanca, Yulia Nathaly Cajas, Rosane Mazzarella, Karina Cañón-Beltrán, Maria Encina Gonzalez Martínez, Dimitrios Rizos","doi":"10.1590/1984-3143-AR2025-0059","DOIUrl":"10.1590/1984-3143-AR2025-0059","url":null,"abstract":"Embryo-maternal communication is a critical process that influences early embryonic development, implantation success, and pregnancy outcomes across mammalian species. This review examines the diverse in vitro systems developed to study this complex dialogue, highlighting their applications, advantages, and limitations. We explore conventional approaches such as two-dimensional (2D) cell cultures, which despite their simplicity, face challenges in replicating the three-dimensional (3D) architecture and cellular functions present in vivo. The review progresses through increasingly sophisticated models, including fluid co-culture systems that incorporate bioactive molecules, explant cultures that maintain tissue architecture, air-liquid interface systems that promote epithelial polarization and differentiation, 3D organoid systems that recapitulate complex structural organization, and organ-on-a-chip platforms that recreate mechanical forces and dynamic conditions. Special attention is given to the emerging role of extracellular vesicles (EVs) as mediators of embryo-maternal communication, transporting crucial molecular signals between the embryo and reproductive tract. By comparing these systems across species and developmental stages, we provide a comprehensive framework for selecting appropriate models based on specific research questions. The integration of these in vitro approaches with advanced analytical techniques offers promising avenues for understanding embryo-maternal cross-talk, potentially leading to improved assisted reproductive technologies and strategies to mitigate early pregnancy loss. As technology advances, the continued refinement of these systems will further illuminate the intricate molecular and cellular mechanisms underlying successful embryo development and implantation.","PeriodicalId":7889,"journal":{"name":"Animal Reproduction","volume":"22 3","pages":"e20250059"},"PeriodicalIF":2.1,"publicationDate":"2025-08-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12419245/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145038923","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The effects of breeding young bulls and cows in vitro. 体外培育幼公牛和母牛的效果。

IF 2.1 4区农林科学

Animal Reproduction Pub Date : 2025-08-11 eCollection Date: 2025-01-01 DOI: 10.1590/1984-3143-AR2025-0047

Marc Andre Sirard

{"title":"The effects of breeding young bulls and cows in vitro.","authors":"Marc Andre Sirard","doi":"10.1590/1984-3143-AR2025-0047","DOIUrl":"10.1590/1984-3143-AR2025-0047","url":null,"abstract":"In the bovine dairy sector, the pursuit of rapid genetic advancement has prompted the adoption of increasingly younger parental figures for both males and females. While physiological limitations and access to gametes impose certain restrictions, the impact of age on gamete quality remains crucial yet poorly understood. We propose that the age effect encompasses environmental factors, which include the metabolic state of the parents and the conditions surrounding gametes and embryos within the reproductive tracts of both sexes. Emerging evidence indicates that this environment significantly influences not only the functionality of gametes and early embryos but also the future phenotype of the offspring. Recent research utilizing transcriptomic and epigenetic molecular analyses in bovine models has demonstrated that the age of both females and males gamete donors, can alter gene expression and programming within the embryo in a similar way that metabolic post partum conditions can. This embryo adaptation to parent's age is similarly noted in variations related to different culture conditions and the in vitro fertilization (IVF) process. A common outcome from these circumstances is the development of embryos operating in \"economy\" mode, where translation, cell division, and ATP production are diminished, seemingly as an anticipated adaptation to environmental conditions. Furthermore, new epidemiological studies have shown that these alterations can lead to distinct phenotypes, particularly in animals conceived through IVF, underscoring the long-term consequences that may unfold later in their lives.","PeriodicalId":7889,"journal":{"name":"Animal Reproduction","volume":"22 3","pages":"e20250047"},"PeriodicalIF":2.1,"publicationDate":"2025-08-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12338970/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144820395","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Extracellular vesicles in female reproduction: from basic research to application. 女性生殖中的细胞外囊泡：从基础研究到应用。

IF 2.1 4区农林科学

Animal Reproduction Pub Date : 2025-08-05 eCollection Date: 2025-01-01 DOI: 10.1590/1984-3143-AR2025-0049

Camila Azzolin de Souza, Gislaine Dos Santos, Schaienni Fontoura Saldanha, Luca Angi Souza, Juliano Coelho da Silveira

{"title":"Extracellular vesicles in female reproduction: from basic research to application.","authors":"Camila Azzolin de Souza, Gislaine Dos Santos, Schaienni Fontoura Saldanha, Luca Angi Souza, Juliano Coelho da Silveira","doi":"10.1590/1984-3143-AR2025-0049","DOIUrl":"10.1590/1984-3143-AR2025-0049","url":null,"abstract":"In vitro embryo production (IVEP) offers an alternative approach for fertility preservation, genetic improvement, and reproductive research. However, in vivo, the female reproductive tract constitutes a dynamic microenvironment that undergoes critical changes crucial to support oocyte maturation, fertilization and embryo development. During IVEP, the absence of maternal-gamete and later maternal-embryo cross-talk can compromises both fertility and embryo development as well as quality. Extracellular vesicles (EVs) derived from the maternal reproductive tract, such as those from follicular fluid, oviductal fluid and uterine fluid, have attracted increasing attention due to their ability to carry bioactive biomolecules and partially restore this bidirectional communication when supplemented during IVEP. Moreover, EVs hold the potential to serve as indicator of the physiological or pathological state of reproductive structures as well as serving as real-time biomarkers. In addition, several studies suggest that EVs offer multiples advantages over conventional synthetics carries, opening new frontiers for modern drug or nucleotide delivery systems. Therefore, this review aims to provide a comprehensive overview of EVs derived from female reproductive tract, exploring their potential applications and challenges in enhancing IVEP outcomes and fertility treatments.","PeriodicalId":7889,"journal":{"name":"Animal Reproduction","volume":"22 3","pages":"e20250049"},"PeriodicalIF":2.1,"publicationDate":"2025-08-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12419269/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145038956","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Pregnancy loss in cattle with emphasis on embryo transfer programs. 牛的妊娠损失，重点是胚胎移植方案。

IF 2.1 4区农林科学

Animal Reproduction Pub Date : 2025-08-05 eCollection Date: 2025-01-01 DOI: 10.1590/1984-3143-AR2025-0045

Roberto Sartori, Mirela Balistrieri, Lucas Oliveira E Silva, Carlos Eduardo Cardoso Consentini, Leonardo de França E Melo, Guilherme Correa de Sousa Pontes, Douglas Gaitkoski

{"title":"Pregnancy loss in cattle with emphasis on embryo transfer programs.","authors":"Roberto Sartori, Mirela Balistrieri, Lucas Oliveira E Silva, Carlos Eduardo Cardoso Consentini, Leonardo de França E Melo, Guilherme Correa de Sousa Pontes, Douglas Gaitkoski","doi":"10.1590/1984-3143-AR2025-0045","DOIUrl":"10.1590/1984-3143-AR2025-0045","url":null,"abstract":"Pregnancy loss (PL) in cattle significantly impacts reproductive efficiency and economic viability of herds. Of particular interest, PL in in vitro embryo production (IVP) systems, represents a major challenge to the success of this technique. Establishment and maintenance of pregnancy is influenced by factors such as fertilization, maternal environment, and embryonic signaling issues. Data on dairy cattle have shown that embryo transfer (ET) may lead to greater initial pregnancy, but greater PL compared to artificial insemination (AI), and the impact of environmental conditions on reproductive outcomes seems to be manageable with proper heat stress mitigation strategies, for example. Data on beef cattle submitted to IVP and ET have shown that recipient cows had greater pregnancy per ET (P/ET) and lower PL compared to recipient heifers, with sex-sorted sperm yielding similar or even greater P/ET than conventional semen. Distinct synchronization protocols for recipients yield different reproductive outcomes, and recipient breed also affects P/ET and PL. Moreover, embryo recipients that express estrus after synchronization, as well as recipients in which better quality embryos are transferred, tend to have greater P/ET and lower PL. These findings highlight the importance of management strategies to improve fertility and reduce PL in embryo recipients.","PeriodicalId":7889,"journal":{"name":"Animal Reproduction","volume":"22 3","pages":"e20250045"},"PeriodicalIF":2.1,"publicationDate":"2025-08-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12419244/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145039019","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Insights into early embryonic development - a personal assessment based on 30 years of experience. 洞察早期胚胎发育-基于30年经验的个人评估。

IF 2.1 4区农林科学

Animal Reproduction Pub Date : 2025-08-04 eCollection Date: 2025-01-01 DOI: 10.1590/1984-3143-AR2025-0017

Urban Besenfelder

{"title":"Insights into early embryonic development - a personal assessment based on 30 years of experience.","authors":"Urban Besenfelder","doi":"10.1590/1984-3143-AR2025-0017","DOIUrl":"10.1590/1984-3143-AR2025-0017","url":null,"abstract":"The collection of early embryonic stages from the donor animal and their in vitro development through to transfer back into a recipient animal has gained enormous importance both in understanding embryo physiology and in its application for breeding purposes. Whereas not so long ago the focus was on embryo retrieval after superovulation from the donor animal, oocyte collection by follicular puncture followed by in vitro production (IVP) is now the main source of bovine embryos. However, as recent years of intensive research have shown, it appears to be very difficult to reproduce the extreme complex in vivo processes under laboratory conditions. Consequently, the quality/developmental capacity of embryos available for cryopreservation/storage/transport and transfer still lags behind that of embryos derived directly from animals. Embryo collection in bovine MOET programs is limited to the success of the animal's hormone treatment, embryo collection itself, and transfer on Day 7. IVP largely bypasses these developmental steps in the animal and focuses primarily on the presence of healthy follicular waves. It uses follicular puncture (ovum pick-up: OPU) to obtain immature oocytes, which undergo a three-stage in vitro process to produce embryos that are transferred to the uterus on Day 7. However, it is now known that important processes take place in the oviduct that have a lasting effect on the further development of the embryo. In animals, however, the development of embryos in the oviduct has not yet received sufficient attention. This review will present some highlights of the use of early embryonic stages from the oviduct in different species, but the scientific work mentioned is also largely based on the recent presentation at the AETE 2023 conference in Heraklion, Greece.","PeriodicalId":7889,"journal":{"name":"Animal Reproduction","volume":"22 3","pages":"e20250017"},"PeriodicalIF":2.1,"publicationDate":"2025-08-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12324157/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144793278","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Effects of maternal temperament on uterine and umbilical blood flow, expression of angiogenic proteins, and fetal growth in pregnant ossimi sheep. 母性气质对妊娠奥西米羊子宫和脐血流量、血管生成蛋白表达及胎儿生长的影响。

IF 1.6 4区农林科学

Animal Reproduction Pub Date : 2025-07-07 eCollection Date: 2025-01-01 DOI: 10.1590/1984-3143-AR2024-0125

Mohammed Elmetwally, Fatma Adlan, Basma Hendam, Alaa Samy, Shaymaa Rezk, Samah Lasheen, Heba Mohram, Sara Shalapy, Reham Fahmy, Amy Jo Montgomery, Yasser Y Lenis, Amira Mostagir

{"title":"Effects of maternal temperament on uterine and umbilical blood flow, expression of angiogenic proteins, and fetal growth in pregnant ossimi sheep.","authors":"Mohammed Elmetwally, Fatma Adlan, Basma Hendam, Alaa Samy, Shaymaa Rezk, Samah Lasheen, Heba Mohram, Sara Shalapy, Reham Fahmy, Amy Jo Montgomery, Yasser Y Lenis, Amira Mostagir","doi":"10.1590/1984-3143-AR2024-0125","DOIUrl":"10.1590/1984-3143-AR2024-0125","url":null,"abstract":"This study aimed to examine the efficacy of Doppler and B-mode sonography in evaluating the impact of maternal temperament on hemodynamic alterations in both the fetus and uterus during ovine gestation in addition to alteration of the angiogenic protein expression and fetal biometry. Twenty Ossimi ewes were divided into two groups, more reactive (MR: 12 sheep) and less reactive (LR: 8 sheep). Several endpoints were assessed every 2 weeks (wk) from breeding to wk 20 of pregnancy. Blood samples were taken to evaluate the expression of angiogenic proteins at parturition. The resistance (RI) and pulsatility (PI) indexes of the uterine (UtA) and fetal umbilical arteries (UMA) were measured. Biometry includes: diameter of amniotic vesicles (AVD), umbilicus (UMD), fetal thoracic diameter (FTD) and metacarpal length (MCL). The UtA-PI was higher in MR compared to LR ewes between 6-12 wks of gestation (P>0.01), while a tendency was recognized at wk 14 (P=0.054). The same was true for UtA-RI during the first 8 wk of pregnancy (P < 0.03) when MR and LR animals were compared. Similarly, UMA-RI was higher in fetuses of MR than LR ewes at wk 14 (P<0.0003) and 20 (P<0.02) of pregnancy. The differences in UMA-PI reached significance at wk 6, 8, 10, and 20 (P<0.0 -0.003). Furthermore, significant changes in fetal biometry were investigated. The expressions of VEGF, NOS3 and HIF 1α were increased in the less reactive sheep (P<0.001). In conclusion, the maternal temperaments affect the Doppler, B mode as well as the expressions of mRNAs for VEGF, NOS3 and HIF 1α genes at time of parturition.","PeriodicalId":7889,"journal":{"name":"Animal Reproduction","volume":"22 2","pages":"e20240125"},"PeriodicalIF":1.6,"publicationDate":"2025-07-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12232499/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144582850","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Reproduction in the frog Aubria subsigillata (Dumeril 1856): hormonal injection and captive breeding technique. 小野蛙的繁殖（Dumeril 1856）：激素注射和圈养繁殖技术。

IF 1.6 4区农林科学

Animal Reproduction Pub Date : 2025-07-07 eCollection Date: 2025-01-01 DOI: 10.1590/1984-3143-AR2024-0129

Houénafa Aimé Chrysostome Gansa, Hyppolite Agadjihouèdé

{"title":"Reproduction in the frog Aubria subsigillata (Dumeril 1856): hormonal injection and captive breeding technique.","authors":"Houénafa Aimé Chrysostome Gansa, Hyppolite Agadjihouèdé","doi":"10.1590/1984-3143-AR2024-0129","DOIUrl":"10.1590/1984-3143-AR2024-0129","url":null,"abstract":"Aubria subsigillata (Dumeril 1856) is a frog under increasing threat that is intensely harvested by Beninese people. The aim of this study was to develop a captive breeding program to repopulate the natural habitat of frogs in Benin Republic in view to conserve anuran biodiversity. The methodology adopted consisted of combining broodstock not injected with ovulin (hCG) in netted cages (natural reproduction) and broodstock injected with ovulin in tanks (assisted reproduction). In vitro fertilization of unfertilized female eggs by male milt was also carried out (controlled reproduction). In natural reproduction, A. subsigillata eggs or tadpoles are not produced. The concentration of 0.2 IU/g administered by intra-femoral injection resulted in the release of gametes in both sexes. Assisted reproduction enabled eggs to be obtained from the three coupling tanks after a post-injection lag time varying between 93 and 94 hours (Tank one = 304 eggs; Tank two = 125 eggs; Tank three = 56 eggs) and at a water temperature of 26°C; however, there was no incubation of eggs. For controlled reproduction, the average time between injection and first spermiation was 13 hours for males and 27 hours for females post-injection at a temperature of 28.5°C. Fecundity varied between 56 and 329 eggs. The eggs had an average weight of 1 mg and were incubated between 164 and 168 hours after fertilization. However, the incubation rate decreased on the 5th day due to infection of the eggs by Saprolegnia sp. These results suggest that the experiment is partially viable: while hormonal stimulation can induce spawning and fertilization under controlled conditions, the success of incubation and development remains limited, highlighting the need for improvements in biosecurity and egg management to ensure full viability of captive breeding efforts.","PeriodicalId":7889,"journal":{"name":"Animal Reproduction","volume":"22 2","pages":"e20240129"},"PeriodicalIF":1.6,"publicationDate":"2025-07-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12232497/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144582852","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Quantification of polyoocyte follicles (POFs) in domestic cats. 家猫多卵母细胞卵泡（POFs）的定量测定。

IF 1.6 4区农林科学

Animal Reproduction Pub Date : 2025-07-04 eCollection Date: 2025-01-01 DOI: 10.1590/1984-3143-AR2024-0127

Alexandra Lays Petri, Mariana Valentini Casagrande, Adalgiza Pinto, Jonatas Cattelam, Camila Keterine Gorzelanski Trenkel, Jacqueline Zanella, Leonardo Gruchouskei

{"title":"Quantification of polyoocyte follicles (POFs) in domestic cats.","authors":"Alexandra Lays Petri, Mariana Valentini Casagrande, Adalgiza Pinto, Jonatas Cattelam, Camila Keterine Gorzelanski Trenkel, Jacqueline Zanella, Leonardo Gruchouskei","doi":"10.1590/1984-3143-AR2024-0127","DOIUrl":"10.1590/1984-3143-AR2024-0127","url":null,"abstract":"Ovarian follicles usually involve only one oocyte. However, the existence of follicles with more than one oocyte has been described in different species. Therefore, this study evaluated the occurrence and number of Polyoocyte Follicles (POFs) in the ovaries of cats undergoing the ovariohysterectomy technique. For this purpose, ovaries from 33 cats were collected and submitted to histological preparation and analysis. The slides were evaluated under a microscope by two evaluators, who quantified the presence of follicles containing one or more oocytes and classified the follicular stage. Monoocyte follicles (MOFs) were found in all ovaries. POFs were observed at similar frequencies in both the right and left ovaries. The frequency of POFs with two oocytes was 79.03% in the right ovaries and 75.75% in the left ovaries. Follicles with three oocytes were found in 16.12% of the right ovaries and 27.27% of the left ovaries. The mean number of follicles with zero, one, two, three or more oocytes was similar between the ovaries, being 6.58, 310.5, 3.25, and 0.29 in the right ovary and 6.25, 312.7, 4.25, and 0.41 in the left ovary (p-values of 0.7569, 0.9654, 0.4785, and 0.5015, respectively). POFs containing from two to five oocytes were identified in different stages of development. POFs at all stages of development were found in both ovaries, and the occurrence of these structures was influenced by the age, number of pregnancies, number of estrus, and weight of the cats studied.","PeriodicalId":7889,"journal":{"name":"Animal Reproduction","volume":"22 2","pages":"e20240127"},"PeriodicalIF":1.6,"publicationDate":"2025-07-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12232498/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144582851","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Effect of dietary supplementation of spray-dried plasma on performance and semen quality in aging broiler breeders. 饲粮中添加喷雾干燥血浆对老龄肉种鸡生产性能和精液质量的影响。

IF 1.6 4区农林科学

Animal Reproduction Pub Date : 2025-06-30 eCollection Date: 2025-01-01 DOI: 10.1590/1984-3143-AR2024-0111

Carlos Alexandre Granghelli, Mário Henrique Scapin Lopes, Luís Rangel, Joy Campbell, Javier Polo, Joe Crenshaw, Eneiva Carla Carvalho Celeghini, Pedro Nacib Jorge-Neto, Helena Lage Ferreira, Vinicius Santos Moura, Jennifer Soutter Motta, Cristiane Soares da Silva Araujo, Lucio Francelino Araujo

{"title":"Effect of dietary supplementation of spray-dried plasma on performance and semen quality in aging broiler breeders.","authors":"Carlos Alexandre Granghelli, Mário Henrique Scapin Lopes, Luís Rangel, Joy Campbell, Javier Polo, Joe Crenshaw, Eneiva Carla Carvalho Celeghini, Pedro Nacib Jorge-Neto, Helena Lage Ferreira, Vinicius Santos Moura, Jennifer Soutter Motta, Cristiane Soares da Silva Araujo, Lucio Francelino Araujo","doi":"10.1590/1984-3143-AR2024-0111","DOIUrl":"10.1590/1984-3143-AR2024-0111","url":null,"abstract":"A total of 216 Cobb 500 broiler breeder hens were randomly distributed across two treatments consisting of diets supplemented with 0 or 1% spray-dried plasma (SDP), resulting in 27 replications per treatment and four birds per pen. In addition, 36 roosters, housed in individual pens, were allocated to the same treatments, with each bird considered a replicate, in order to assess the influence of SDP on semen and hatching characteristics. The experimental diets were fed from 26 weeks until the conclusion of the study, at 65 weeks of age. Semen quality, embryonic mortality and quality of newly hatched chicks were evaluated at 29, 45, and 63 weeks. Hens were inseminated with fresh semen, and eggs were incubated following standard procedures. Semen from 63-week-old roosters had higher sperm concentration than other age groups, while 29-week-old rooster semen exhibited higher progressive motility than semen from older roosters (P < 0.001). The 45-week-old roosters had the lowest values for the analyzed semen quality parameters (average path velocity, straight-line velocity and curvilinear velocity). Additionally, sperm from 45-week-old roosters, regardless of SDP supplementation, had fewer total morphological defects than 63-week-old roosters. However, 1% SDP in the diet reduced total sperm cell defects at 63 weeks of age, as well as total sperm head and tail defects (P < 0.05) compared to unsupplemented birds. Dietary SDP reduced late embryonic death in 63-week-old breeders (P < 0.05). The results demonstrate that 1% SDP in breeder diets reduced late embryonic mortality and sperm cell defects, mainly in aged birds, enhancing the reproductive performance of broiler breeders.","PeriodicalId":7889,"journal":{"name":"Animal Reproduction","volume":"22 2","pages":"e20240111"},"PeriodicalIF":1.6,"publicationDate":"2025-06-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12212462/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144537805","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0