Animal Reproduction最新文献_第3页

Effect of dietary supplementation of spray-dried plasma on performance and semen quality in aging broiler breeders. 饲粮中添加喷雾干燥血浆对老龄肉种鸡生产性能和精液质量的影响。

IF 1.6 4区农林科学

Animal Reproduction Pub Date : 2025-06-30 eCollection Date: 2025-01-01 DOI: 10.1590/1984-3143-AR2024-0111

Carlos Alexandre Granghelli, Mário Henrique Scapin Lopes, Luís Rangel, Joy Campbell, Javier Polo, Joe Crenshaw, Eneiva Carla Carvalho Celeghini, Pedro Nacib Jorge-Neto, Helena Lage Ferreira, Vinicius Santos Moura, Jennifer Soutter Motta, Cristiane Soares da Silva Araujo, Lucio Francelino Araujo

{"title":"Effect of dietary supplementation of spray-dried plasma on performance and semen quality in aging broiler breeders.","authors":"Carlos Alexandre Granghelli, Mário Henrique Scapin Lopes, Luís Rangel, Joy Campbell, Javier Polo, Joe Crenshaw, Eneiva Carla Carvalho Celeghini, Pedro Nacib Jorge-Neto, Helena Lage Ferreira, Vinicius Santos Moura, Jennifer Soutter Motta, Cristiane Soares da Silva Araujo, Lucio Francelino Araujo","doi":"10.1590/1984-3143-AR2024-0111","DOIUrl":"10.1590/1984-3143-AR2024-0111","url":null,"abstract":"A total of 216 Cobb 500 broiler breeder hens were randomly distributed across two treatments consisting of diets supplemented with 0 or 1% spray-dried plasma (SDP), resulting in 27 replications per treatment and four birds per pen. In addition, 36 roosters, housed in individual pens, were allocated to the same treatments, with each bird considered a replicate, in order to assess the influence of SDP on semen and hatching characteristics. The experimental diets were fed from 26 weeks until the conclusion of the study, at 65 weeks of age. Semen quality, embryonic mortality and quality of newly hatched chicks were evaluated at 29, 45, and 63 weeks. Hens were inseminated with fresh semen, and eggs were incubated following standard procedures. Semen from 63-week-old roosters had higher sperm concentration than other age groups, while 29-week-old rooster semen exhibited higher progressive motility than semen from older roosters (P < 0.001). The 45-week-old roosters had the lowest values for the analyzed semen quality parameters (average path velocity, straight-line velocity and curvilinear velocity). Additionally, sperm from 45-week-old roosters, regardless of SDP supplementation, had fewer total morphological defects than 63-week-old roosters. However, 1% SDP in the diet reduced total sperm cell defects at 63 weeks of age, as well as total sperm head and tail defects (P < 0.05) compared to unsupplemented birds. Dietary SDP reduced late embryonic death in 63-week-old breeders (P < 0.05). The results demonstrate that 1% SDP in breeder diets reduced late embryonic mortality and sperm cell defects, mainly in aged birds, enhancing the reproductive performance of broiler breeders.","PeriodicalId":7889,"journal":{"name":"Animal Reproduction","volume":"22 2","pages":"e20240111"},"PeriodicalIF":1.6,"publicationDate":"2025-06-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12212462/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144537805","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Effects of cactus (Opuntia ficus-indica) mucilage on boar sperm cryosurvival. 仙人掌（Opuntia ficus-indica）粘液对野猪精子低温存活的影响。

IF 1.6 4区农林科学

Animal Reproduction Pub Date : 2025-06-30 eCollection Date: 2025-01-01 DOI: 10.1590/1984-3143-AR2024-0004

Juan Antonio Ramírez-Chequer, Marco Antonio Lopez-Carlos, Carlos Fernando Arechiga-Flores, Pedro Hernandez-Briano, Carlos Aurelio Medina-Flores, Fabiola Mendez-Llorente

{"title":"Effects of cactus (Opuntia ficus-indica) mucilage on boar sperm cryosurvival.","authors":"Juan Antonio Ramírez-Chequer, Marco Antonio Lopez-Carlos, Carlos Fernando Arechiga-Flores, Pedro Hernandez-Briano, Carlos Aurelio Medina-Flores, Fabiola Mendez-Llorente","doi":"10.1590/1984-3143-AR2024-0004","DOIUrl":"10.1590/1984-3143-AR2024-0004","url":null,"abstract":"This study aimed to evaluate the effect of three inclusion levels of Opuntia ficus-indica mucilage (OFM) to the lactose-egg yolk extender on the quality of frozen-thawed boar semen. Semen samples (n=22) were split into four OFM treatments: 0.0 (control), 3.3, 6.7, or 10.0% OFM. The semen was packaged in 0.5 mL straws and frozen using conventional methods. The sperm motility, viability, membrane integrity (HOST), and morphology (intact acrosome and normal sperm) characteristics were evaluated immediately post-thaw. Additionally, sperm were incubated at 37 ºC during 3.5 h and monitored hourly in a termoresistance test (TRT) to assess sperm motility, viability, and membrane integrity, The study showed that adding OFM to the freezing extender significantly improved (P < 0.05) sperm quality characteristics, without affecting (P > 0.05) acrosome integrity or sperm abnormalities. Furthermore, the OFM addition improved (P < 0.05) the sperm viability and membrane integrity for up to 3.5 h of incubation. Results indicate that OFM can be added to the extender at a 6.7% OFM inclusion level to improve the quality of cryopreserved boar sperm.","PeriodicalId":7889,"journal":{"name":"Animal Reproduction","volume":"22 2","pages":"e20240004"},"PeriodicalIF":1.6,"publicationDate":"2025-06-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12212463/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144537806","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Melatonin mitigates PGF-induced apoptosis during luteal regression in heat-exposed rats. 褪黑素减轻热暴露大鼠黄体退化过程中pgf诱导的细胞凋亡。

IF 1.6 4区农林科学

Animal Reproduction Pub Date : 2025-06-30 eCollection Date: 2025-01-01 DOI: 10.1590/1984-3143-AR2024-0122

Hadi Tavakolikazerooni, Hao Yu, Saif Ullah, Wael Ennab, Dagan Mao

{"title":"Melatonin mitigates PGF-induced apoptosis during luteal regression in heat-exposed rats.","authors":"Hadi Tavakolikazerooni, Hao Yu, Saif Ullah, Wael Ennab, Dagan Mao","doi":"10.1590/1984-3143-AR2024-0122","DOIUrl":"10.1590/1984-3143-AR2024-0122","url":null,"abstract":"This study investigates the protective effects of melatonin against heat exposure during PGF-induced luteal regression in rats. Seventy-five PMSG and hCG primed rats were divided into three groups: non-heat-exposure (NHE), heat-exposure (HE), and melatonin plus heat-exposure (M+HE). The HE group underwent daily heat exposure (41°C for 2 h) for 7 days, while the M+HE group received intraperitoneal injection of melatonin (10 mg/kg body weight) before each heat session. On Day 7, PGF was administered, and ovarian samples were collected at 0, 1, 2, 8, and 24 h post-PGF. One set of ovaries was processed for histological analysis, including H&E staining, immunohistochemistry, and transmission electron microscopy and the other set was processed for Western blot for apoptotic protein expression. Results showed that heat exposure increased ovarian weight, disrupted follicular development, and elevated ovarian apoptotic markers (Caspase-3 and Bax), leading to luteal cell damage. Melatonin preserved ovarian weight, improved follicular and luteal structure, reduced atretic follicles, and mitigated luteal cell degeneration. In addition, melatonin decreased apoptotic marker expression and the Bax/Bcl-2 ratio, particularly at 16 and 24 h. These findings suggest melatonin protects luteal cells from heat-induced apoptosis during PGF-triggered regression, supporting reproductive function.","PeriodicalId":7889,"journal":{"name":"Animal Reproduction","volume":"22 2","pages":"e20240122"},"PeriodicalIF":1.6,"publicationDate":"2025-06-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12212464/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144537807","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

An integrated analysis of second- and third-generation transcriptome sequencing technologies reveals the DAZAP1 function in pig testis. 对第二代和第三代转录组测序技术的综合分析揭示了DAZAP1在猪睾丸中的功能。

IF 1.6 4区农林科学

Animal Reproduction Pub Date : 2025-06-27 eCollection Date: 2025-01-01 DOI: 10.1590/1984-3143-AR2024-012

Xia Zhang, Hailong Huo, Honglin Li, Yongqing Liu, Fujie Qiao, Changyao Li, Jinlong Huo

{"title":"An integrated analysis of second- and third-generation transcriptome sequencing technologies reveals the DAZAP1 function in pig testis.","authors":"Xia Zhang, Hailong Huo, Honglin Li, Yongqing Liu, Fujie Qiao, Changyao Li, Jinlong Huo","doi":"10.1590/1984-3143-AR2024-012","DOIUrl":"10.1590/1984-3143-AR2024-012","url":null,"abstract":"The quality of pig sperm is one of the crucial determinants of reproductive ability, and sperm defects can shorten the reproductive life of boars and affect the production of offspring. During transcription and translation, the DAZAP1 gene exerts regulatory control over alternative splicing, thereby exerting influence on vital cellular processes including cell growth, development, and spermatogenesis. In this study, we employed second- and third-generation transcriptome sequencing techniques to isolate and identify the DAZAP1 gene and its transcripts using Banna mini-pig inbred line (BMI) testicular cDNA as a template. We identified three splice variants of the DAZAP1 gene, including ENSSSCT00000023438.4 (DAZAP1_X1), ENSSSCT00000051975.3 (DAZAP1_X2), and ENSSSCT00000074738.2 (DAZAP1_X3). Furthermore, the transcript DAZAP1_X2, was subjected to comprehensive analysis. The DAZAP1_X2 variant comprises 13 exons, with a coding sequence (CDS) length of 1254 bp (417 aa). Subsequently, enrichment analyses based on GO and KEGG pathways revealed that DAZAP1_X2 primarily participated in pathways associated with spermatogenesis, movement of the 9+2 cilium structure, germ cell development, gamete generation, and sexual reproduction. The ceRNA analysis identified three miRNAs interacting with DAZAP1_X2: ssc-miR-107, ssc-miR-127, and ssc-miR-1343, which were primarily linked to spermatogenesis. Both the testis and urethral bulb had significant levels of DAZAP1 expression, according to multi-tissue expression analysis. Subcellular localization indicated that the DAZAP1 was mainly distributed in the cell nucleus. DAZAP1 was critical for sperm formation and was essential for reproductive. These results shed light on the biological roles of DAZAP1 in pigs.","PeriodicalId":7889,"journal":{"name":"Animal Reproduction","volume":"22 2","pages":"e20240141"},"PeriodicalIF":1.6,"publicationDate":"2025-06-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12203886/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144526143","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

β-hydroxybutyrate induces endoplasmic reticulum stress in bovine cumulus cells during oocyte maturation in vitro. β-羟基丁酸诱导牛卵丘细胞体外成熟过程中的内质网应激。

IF 1.6 4区农林科学

Animal Reproduction Pub Date : 2025-05-30 eCollection Date: 2025-01-01 DOI: 10.1590/1984-3143-AR2024-0106

Daniele Missio, Julia Koch, Zigomar da Silva, Francielli Weber Santos Cibin, Marcos Henrique Barreta, Vitor Braga Rissi, André Lucio Fontana Goetten, Fernando Silveira Mesquita, Valério Marques Portela, Paulo Bayard Dias Gonçalves, Rogério Ferreira

{"title":"β-hydroxybutyrate induces endoplasmic reticulum stress in bovine cumulus cells during oocyte maturation in vitro.","authors":"Daniele Missio, Julia Koch, Zigomar da Silva, Francielli Weber Santos Cibin, Marcos Henrique Barreta, Vitor Braga Rissi, André Lucio Fontana Goetten, Fernando Silveira Mesquita, Valério Marques Portela, Paulo Bayard Dias Gonçalves, Rogério Ferreira","doi":"10.1590/1984-3143-AR2024-0106","DOIUrl":"10.1590/1984-3143-AR2024-0106","url":null,"abstract":"Ketosis is a significant metabolic disorder caused by negative energy balance (NEB). β-hydroxybutyrate (BHBA) is the most abundant circulating ketone body and is known to accumulate within follicular fluid. Although NEB and metabolic stress conditions have been associated with reduced fertility in cows, the effect of increased BHBA on the viability and functionality of bovine cumulus-oocyte complexes (COC) is poorly understood. The aim of this study was to determine the effects of BHBA on expansion, oxidative status, and endoplasmic reticulum (ER) stress in cumulus cells, oocyte nuclear maturation, and in vitro embryo production (IVP) in cattle. Cumulus-oocyte complexes were treated with 0, 2, or 4 mM of BHBA for 6, 12, 18, or 24 h during oocyte in vitro maturation (IVM.) Cumulus expansion and mRNA levels of genes related to cumulus expansion, oxidative status, ER stress, and autophagy were evaluated. The oocytes were fixed for nuclear maturation analysis at 24 h of IVM. In addition, after IVM with BHBA for 24 hours, cleavage and blastocyst rates were examined. No difference was observed in the rates of oocytes reaching metaphase II, cleavage, and blastocysts. Furthermore, the oxidative status and relative abundance of mRNA for genes associated with antioxidant enzymes and autophagy were not altered by BHBA in cumulus cells. However, BHBA altered the total area of the COC and increased the mRNA levels of genes associated with ER. In conclusion, BHBA affects the expansion and induces ER stress in bovine cumulus cells during IVM without compromising oocyte nuclear maturation, oxidative status, cleavage, and blastocyst rates.","PeriodicalId":7889,"journal":{"name":"Animal Reproduction","volume":"22 2","pages":"e20240106"},"PeriodicalIF":1.6,"publicationDate":"2025-05-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12140510/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144233008","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Effects of the modified "Yijing Fang" on semen quality and reproductive hormones in male yaks with kidney yang deficiency: a focus on mitochondrial apoptosis pathways. 益精方对肾阳虚雄性牦牛精液质量及生殖激素的影响——以线粒体凋亡途径为重点

IF 1.6 4区农林科学

Animal Reproduction Pub Date : 2025-05-26 eCollection Date: 2025-01-01 DOI: 10.1590/1984-3143-AR2024-0139

Panfeng Chang, Hua Wang

引用次数: 0

Double artificial insemination in sheep: a comprehensive review. 绵羊双人工授精技术综述。

IF 1.6 4区农林科学

Animal Reproduction Pub Date : 2025-05-19 eCollection Date: 2025-01-01 DOI: 10.1590/1984-3143-AR2024-0055

Gabriel Maggi, Fabiane Pereira de Moraes, Fernando Caetano de Oliveira, Sergio Farias Vargas, Arnaldo Diniz Vieira, Rafael Gianella Mondadori, Bernardo Garziera Gasperin

{"title":"Double artificial insemination in sheep: a comprehensive review.","authors":"Gabriel Maggi, Fabiane Pereira de Moraes, Fernando Caetano de Oliveira, Sergio Farias Vargas, Arnaldo Diniz Vieira, Rafael Gianella Mondadori, Bernardo Garziera Gasperin","doi":"10.1590/1984-3143-AR2024-0055","DOIUrl":"10.1590/1984-3143-AR2024-0055","url":null,"abstract":"Artificial insemination (AI) in sheep presents variable results, especially when combined with estrus induction treatments during the anestrous period. Alternatives for obtaining better results without significantly altering the costs of hormonal protocols are essential because of the importance of this biotechnology in production systems. One alternative that potentially meets these requirements is double-AI. Therefore, this article aims to review the literature on double-cervical AI in sheep and identify gaps in existing knowledge. Double cervical superficial (CS) AI with frozen-thawed (F.T.) semen after estrus detection significantly increased pregnancy rates (PR) in most (6/8) evaluated studies, with an increase of 7 to 34.2 percentual points (p.p.), compared to single AI. Regarding fixed-time AI (FTAI), all studies used fresh (F) or chilled (C) semen, and no positive effects were observed for double FTAI in most cases (8/9). Most studies have not applied current estrous synchronization protocols and insemination doses. Therefore, further studies are needed to evaluate the potential benefits of double FTAI, especially using F.T. semen in combination with hormonal protocols and insemination doses aligned with current practices.","PeriodicalId":7889,"journal":{"name":"Animal Reproduction","volume":"22 2","pages":"e20240055"},"PeriodicalIF":1.6,"publicationDate":"2025-05-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12091998/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144109336","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Egg-yolk- and a liposome-based extenders: refrigeration time and effects on ram semen quality. 以蛋黄和脂质体为基础的填充剂：冷藏时间及对公羊精液质量的影响。

IF 1.6 4区农林科学

Animal Reproduction Pub Date : 2025-05-09 eCollection Date: 2025-01-01 DOI: 10.1590/1984-3143-AR2024-0052

Rogério Araújo de Almeida, Luan Sitó da Silva, Luiz Gustavo Ferreira de Lima, Flávio Augusto Lourencetti, Camila Freitas-Dell Aqua, João Carlos Pinheiro Ferreira, Eunice Oba

{"title":"Egg-yolk- and a liposome-based extenders: refrigeration time and effects on ram semen quality.","authors":"Rogério Araújo de Almeida, Luan Sitó da Silva, Luiz Gustavo Ferreira de Lima, Flávio Augusto Lourencetti, Camila Freitas-Dell Aqua, João Carlos Pinheiro Ferreira, Eunice Oba","doi":"10.1590/1984-3143-AR2024-0052","DOIUrl":"https://doi.org/10.1590/1984-3143-AR2024-0052","url":null,"abstract":"Sperm cells require time to adjust to lower temperatures. While some studies have established stabilization periods ranging from two to four hours for ovine semen, the exploration of longer periods of stabilization remains limited. The objective of the study is to evaluate the 12-hour refrigeration curve during ovine semen cryopreservation and the impact of a diluent medium containing egg yolk and liposomes. Eight mixed-breed rams (½ Dorper and ½ Santa Inês) provided two ejaculates each, divided into two groups. Group A used a commercial egg yolk-based diluent (Botu-Bov® - Botupharma Ltda, Brazil), while Group B used a commercial liposome-based diluent (OptiXcell®, IMV Technologies, France). Semen was packaged in French straws, cooled, cryopreserved, and thawed for analysis. Group A exhibited superior values (P < 0.05) in progressive motility (MP), progressive linear velocity (VSL), straightness (STR), and linearity (LIN) post-thawing and after 3 hours at 37°C (TTR). Conversely, Group B showed higher (P < 0.05) values for path velocity (VAP), curvilinear velocity (VCL), lateral head displacement amplitude (ALH) post-thawing, and VAP, VSL, VCL, and ALH after TTR. Flow cytometry revealed Group A's higher (P > 0.05) plasma and acrosomal membrane integrity and membrane stability. However, Group B exhibited greater (P > 0.05) superoxide anion generation and lipid peroxidation, indicative of higher oxidative stress. In conclusion, the egg yolk-based diluent outperformed the diluent containing liposomes in sperm kinetic parameters evaluated by CASA, although liposomes showed increased oxidative stress, 12 hours of refrigeration at 5.0°C is an alternative viable for semen cryopreservation in sheep.","PeriodicalId":7889,"journal":{"name":"Animal Reproduction","volume":"22 2","pages":"e20240052"},"PeriodicalIF":1.6,"publicationDate":"2025-05-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12068371/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143956422","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

High-intensity interval training reduces inflammatory mediator levels in the testes of spontaneously hypertensive rats. 高强度间歇训练降低自发性高血压大鼠睾丸炎症介质水平。

IF 1.6 4区农林科学

Animal Reproduction Pub Date : 2025-05-09 eCollection Date: 2025-01-01 DOI: 10.1590/1984-3143-AR2024-0024

Ronivania Jenuario Silva Nespolo, Allice Santos Cruz Veras, Lauren Chrys Soato Marin, Margarete Jardinetti de Oliveira, Aline de Oliveira Santos, Evellin Heloisa Paulineli Pereira, Francilene Lima Agostinho de Souza, Francis Lopes Pacagnelli, Caliê Castilho, Giovana Rampazzo Teixeira, Robson Chacon Castoldi, Ines Cristina Giometti

{"title":"High-intensity interval training reduces inflammatory mediator levels in the testes of spontaneously hypertensive rats.","authors":"Ronivania Jenuario Silva Nespolo, Allice Santos Cruz Veras, Lauren Chrys Soato Marin, Margarete Jardinetti de Oliveira, Aline de Oliveira Santos, Evellin Heloisa Paulineli Pereira, Francilene Lima Agostinho de Souza, Francis Lopes Pacagnelli, Caliê Castilho, Giovana Rampazzo Teixeira, Robson Chacon Castoldi, Ines Cristina Giometti","doi":"10.1590/1984-3143-AR2024-0024","DOIUrl":"https://doi.org/10.1590/1984-3143-AR2024-0024","url":null,"abstract":"Hypertension is an age-related pathology that causes a decline in the function of all organ systems, including the reproductive system, due to its association with increased oxidative stress and inflammation. The inflammatory cytokine levels increase as a result of hypertension and cause inflammation and tissue injury. Although high-intensity interval training (HIIT) has shown promise as a nondrug treatment for hypertensive individuals, its effects on the reproductive system of hypertensive individuals remain unknown. The aim of this study was to investigate the effects of HIIT on plasma hormone concentrations and the expression of inflammatory mediators in the testes of spontaneously hypertensive rats (SHRs). Male SHRs were divided into 2 groups: SHR (control, n=8) and HIIT (SHRs subjected to HIIT on a treadmill for 8 weeks, n=9) groups. The expression of inflammatory mediators (TNFα and IL-6) in the testes and testosterone, prolactin, and corticosterone concentrations in plasma were measured. No difference in TNFα expression was found between the groups. The groups also showed no significant differences in hormone levels. However, SHRs that underwent HIIT showed lower immunostaining for IL-6 in their testes than did SHRs that did not undergo HIIT training (P < 0.05) and the HIIT group presented lower lower systolic blood pressure than did the SHR group. We concluded that HIIT for two months reduces the BSP and IL-6 levels in the testes of hypertensive rats.","PeriodicalId":7889,"journal":{"name":"Animal Reproduction","volume":"22 2","pages":"e20240024"},"PeriodicalIF":1.6,"publicationDate":"2025-05-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12068372/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143956432","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Season-specific effects of α-tocopherol supplementation during bovine oocyte in vitro maturation on embryo yield and quality. 牛卵母细胞体外成熟过程中补充α-生育酚对胚胎产量和品质的季节特异性影响。

IF 1.6 4区农林科学

Animal Reproduction Pub Date : 2025-05-09 eCollection Date: 2025-01-01 DOI: 10.1590/1984-3143-AR2024-0136

Bruno Barcelona, Zully Ramos, Carolina Viñoles, Nélida Rodríguez-Osorio, Francisco Báez

{"title":"Season-specific effects of α-tocopherol supplementation during bovine oocyte in vitro maturation on embryo yield and quality.","authors":"Bruno Barcelona, Zully Ramos, Carolina Viñoles, Nélida Rodríguez-Osorio, Francisco Báez","doi":"10.1590/1984-3143-AR2024-0136","DOIUrl":"https://doi.org/10.1590/1984-3143-AR2024-0136","url":null,"abstract":"Elevated temperature-humidity index (THI) levels, common in subtropical summers, can impair bovine oocyte development by increasing reactive oxygen species (ROS) accumulation, leading to oxidative stress and reduced developmental competence. Alpha-tocopherol, a potent antioxidant, has the potential to mitigate these effects by scavenging ROS. However, its seasonal efficacy during bovine oocyte in vitro maturation (IVM) remains underexplored. This study evaluated the impact of 100 µM α-tocopherol supplementation during IVM on oocytes collected in spring (THI: 68.7±3) and summer (THI: 73±3) in Northern Uruguay. Oocytes underwent IVM, fertilization, and embryos were cultured in vitro until day 9 post-fertilization. Blastocysts were assessed for ROS levels, apoptosis, and the abundance of transcripts linked to development and oxidative stress. Results showed a season-specific response to α-tocopherol supplementation. While no significant effects were observed in spring, summer oocytes exhibited increased maturation, cleavage, and blastocyst rates, along with improved blastocyst quality characterized by reduced apoptosis and lower BAX transcript levels. These findings indicate that α-tocopherol supplementation during IVM enhances oocyte developmental competence under heat stress conditions, supporting its potential as a strategy to mitigate oxidative damage and improve bovine embryo production during summer.","PeriodicalId":7889,"journal":{"name":"Animal Reproduction","volume":"22 2","pages":"e20240136"},"PeriodicalIF":1.6,"publicationDate":"2025-05-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12068370/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143958440","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0