Animal ReproductionPub Date : 2024-08-12eCollection Date: 2024-01-01DOI: 10.1590/1984-3143-AR2024-0071
Yann Locatelli, Colin Vion, Nicolas Duffard, Nicolas Bon, Jérémy Bernard, Charly Binaud, Nina Trontti, Gérard Baril, Pascal Mermillod
{"title":"Successful transposition of sperm freezing, in vitro fertilization and artificial insemination methodologies in the Tadjik markhor species (<i>Capra falconeri heptneri</i>).","authors":"Yann Locatelli, Colin Vion, Nicolas Duffard, Nicolas Bon, Jérémy Bernard, Charly Binaud, Nina Trontti, Gérard Baril, Pascal Mermillod","doi":"10.1590/1984-3143-AR2024-0071","DOIUrl":"10.1590/1984-3143-AR2024-0071","url":null,"abstract":"<p><p>The objective of the present study was to transpose sperm freezing methodology from domestic goat to the Tadjik markhor (<i>Capra falconeri heptneri</i>) and to address the feasibility to develop IVP and artificial insemination using such frozen semen. Semen of different adult markhor males were successfully recovered by electro-ejaculation and were then frozen using caprine methodology. Frozen semen showed good survival rates at thawing and good fertility rates were assessed in heterologous <i>in vitro</i> fertilization system with goat oocytes. LOPU/IVF was applied for Tadjik markhor females allowing the first successful blastocyst production <i>in vitro</i>. In an applied program, we also transposed successfully intrauterine AI method with frozen/thawed semen to the Tadjik markhor.</p>","PeriodicalId":7889,"journal":{"name":"Animal Reproduction","volume":"21 3","pages":"e20240071"},"PeriodicalIF":1.6,"publicationDate":"2024-08-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11340789/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142035032","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"The microbiome effect on the female reproductive performance.","authors":"Ricardo Zanella, Janine de Camargo, Claudia Almeida Scariot, Mariana Groke Marques","doi":"10.1590/1984-3143-AR2024-0063","DOIUrl":"10.1590/1984-3143-AR2024-0063","url":null,"abstract":"<p><p>The female reproductive function is coordinated by the endocrine system driven by the hypothalamic-pituitary-gonadal (HPG) axis. While not directly part of the female reproductive system, the gut microbiome plays a crucial role in overall health, including reproductive health. The gut microbiome communicates bidirectionally with the brain via the gut-brain axis, influencing stress levels, mood, and hormonal balance, which can impact reproductive health and fertility. In addition to that, the vaginal and uterine microbiome are directly involved with the reproductive success of farm animals, including female fertility and offspring development. In this paper, we summarize some of the effects of bacterial contamination in the female reproductive tract and their association with reproductive performance in farm animals.</p>","PeriodicalId":7889,"journal":{"name":"Animal Reproduction","volume":"21 3","pages":"e20240063"},"PeriodicalIF":1.6,"publicationDate":"2024-08-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11340800/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142035033","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Animal ReproductionPub Date : 2024-08-12eCollection Date: 2024-01-01DOI: 10.1590/1984-3143-AR2024-0032
Marcelo Marcondes Seneda, Camila Bortoliero Costa, Amanda Fonseca Zangirolamo, Mariana Moreira Dos Anjos, Gabriela Rodrigues de Paula, Fábio Morotti
{"title":"From the laboratory to the field: how to mitigate pregnancy losses in embryo transfer programs?","authors":"Marcelo Marcondes Seneda, Camila Bortoliero Costa, Amanda Fonseca Zangirolamo, Mariana Moreira Dos Anjos, Gabriela Rodrigues de Paula, Fábio Morotti","doi":"10.1590/1984-3143-AR2024-0032","DOIUrl":"10.1590/1984-3143-AR2024-0032","url":null,"abstract":"<p><p>Pregnancy losses negatively affect the cattle industry, impacting economic indices and consequently the entire production chain. Early embryonic failure has been an important challenge in the embryo industry because proper identification of embryo death at the beginning of gestation is difficult. This review aimed to provide a better understanding on reproductive failure and the relationship between early embryonic loss and different reproductive biotechniques. This review also considers insights and possible strategies for reducing early embryonic loss. The strategies addressed are as follows: i) great impact of rigorous embryo evaluation on reducing embryo losses; ii) selection of recipients at the time of transfer, taking into account health and nutritional status, and classification of the corpus luteum using ultrasound, either in area or vascularization; and iii) paternal effect as one of the factors that contribute to pregnancy losses, with a focus on embryo transfer.</p>","PeriodicalId":7889,"journal":{"name":"Animal Reproduction","volume":"21 3","pages":"e20240032"},"PeriodicalIF":1.6,"publicationDate":"2024-08-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11340798/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142034957","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Animal ReproductionPub Date : 2024-08-05eCollection Date: 2024-01-01DOI: 10.1590/1984-3143-AR2024-0042
John James Bromfield
{"title":"Interaction of semen with female reproductive tract tissues: what we know, what we guess and what we need to do.","authors":"John James Bromfield","doi":"10.1590/1984-3143-AR2024-0042","DOIUrl":"10.1590/1984-3143-AR2024-0042","url":null,"abstract":"<p><p>For nearly 100 years the postcoital inflammatory response has been described in the female reproductive tract of rodents. Since the 1950's this observation has been made in a number of animals including humans and domestic species. Yet pregnancy can be initiated and maintained by using embryo transfer which bypasses insemination and the related postcoital inflammatory response. Thus, the role of semen exposure beyond sperm transport and subsequent postcoital inflammatory response in female reproductive tissues has yet to be given a true physiological purpose. Historically the postcoital inflammatory response of female tissues was suggested to remove spermatozoa and male derived pathogens from the female reproductive tract. More recently, semen exposure and the postcoital inflammatory response have been suggested to play a role in long-term preparation of the maternal immune system to the semi-allogeneic pregnancy, ancillary support of the preimplantation embryo, and potentially fetal programing that improves pregnancy outcomes, while the absence or inappropriate postcoital inflammation has been suggested to contribute to pregnancy complications. Although the postcoital inflammatory response has been robustly characterized, the evidence for its role in promoting positive pregnancy outcomes or reducing pregnancy complications remains tenuous. This manuscript is designed to balance the information we know regarding semen exposure and postcoital inflammation in various animal systems, with the information we perceive to be factual but perhaps not yet fully tested, along with the data we have yet to generate if we intend to postulate a physiological purpose of the postcoital inflammatory response to pregnancy outcomes.</p>","PeriodicalId":7889,"journal":{"name":"Animal Reproduction","volume":"21 3","pages":"e20240042"},"PeriodicalIF":1.6,"publicationDate":"2024-08-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11340795/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142034959","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Animal ReproductionPub Date : 2024-08-05eCollection Date: 2024-01-01DOI: 10.1590/1984-3143-AR2024-0018
Caio Sérgio Santos, Yasmim Carla da Silva Cavalcante, Lívia Batista Campos, Andréia Maria da Silva, Francisco Marlon Carneiro Feijó, Alexandre Rodrigues Silva
{"title":"Investigating the safety of antibiotics added to collared peccary (<i>Pecari tajacu</i>) semen extender through a multiparametric thermoresistance test.","authors":"Caio Sérgio Santos, Yasmim Carla da Silva Cavalcante, Lívia Batista Campos, Andréia Maria da Silva, Francisco Marlon Carneiro Feijó, Alexandre Rodrigues Silva","doi":"10.1590/1984-3143-AR2024-0018","DOIUrl":"10.1590/1984-3143-AR2024-0018","url":null,"abstract":"<p><p>The effects of antibiotics on sperm longevity in collared peccary (<i>Pecari tajacu</i>) fresh diluted semen was evaluated. Semen samples from six adult males were collected by electroejaculation and diluted in Tris-citrate-fructose alone (control) and plus streptomycin-penicillin (2 mg/ml-2000 IU/ml) or gentamicin (70 µg/ml). Membrane integrity and functionality, mitochondrial activity and sperm morphology were assessed subjectively. Sperm motility and other kinetic parameters were objectively assessed using CASA (computer-assisted semen analysis). The semen diluted according to the treatments were submitted to the thermoresistance test, incubated at 37 ° C, and the sperm parameters analyzed at 0, 30, 60, 120 and 180 min. The average values of the treatments were compared with each other and between the times. There were no differences (P > 0.05) between treatments until the end of the test. Control and streptomycin-penicillin samples maintained sperm function for up to 180 min (with total motility of 24.3 ± 7.1% and 28 ± 8.7%, respectively). Gentamicin aliquots retained most parameters until the end of the incubation, except for membrane integrity and mitochondrial activity that declined (P < 0.05) at 180 min (53.1 ± 7.1% and 50.7 ± 6.2%, respectively) compared to 0 min (80.5 ± 4.7% and 86.3 ± 3.4%, respectively). In conclusion, a multiparametric thermoresistance test proved that Tris-based extenders used for collared peccary semen can be effectively supplemented by streptomycin-penicillin (2 mg/ml-2000 IU/ml) or gentamicin (70 µg/ml), especially during 180-min incubation at 37 °C.</p>","PeriodicalId":7889,"journal":{"name":"Animal Reproduction","volume":"21 3","pages":"e20240018"},"PeriodicalIF":1.6,"publicationDate":"2024-08-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11340791/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142034960","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Livestock embryonic stem cells for reproductive biotechniques and genetic improvement.","authors":"Micaela Navarro, Lucia Laiz-Quiroga, Carolina Blüguermann, Adrián Mutto","doi":"10.1590/1984-3143-AR2024-0029","DOIUrl":"10.1590/1984-3143-AR2024-0029","url":null,"abstract":"<p><p>Embryonic stem cells (ESCs) have proven to be a great <i>in vitro</i> model that faithfully recapitulates the events that occur during <i>in vivo</i> embryogenesis, making them a unique tool to study the cellular and molecular mechanisms that define tissue specification during embryonic development. Livestock ESCs are particularly attractive and have broad prospects including drug selection and human disease modeling, improvement of reproductive biotechniques and agriculture-related applications such as production of genetically modified animals. While mice and human ESCs have been established many years ago, no significant advances were made in livestock species until recently. Nowadays, livestock ESCs are available from cattle, pigs, sheep, horses and rabbits with different states of pluripotency. In this review, we summarize the current advances on livestock ESCs establishment and maintenance along with their present and future applications.</p>","PeriodicalId":7889,"journal":{"name":"Animal Reproduction","volume":"21 3","pages":"e20240029"},"PeriodicalIF":1.6,"publicationDate":"2024-08-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11340801/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142034961","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Animal ReproductionPub Date : 2024-08-05eCollection Date: 2024-01-01DOI: 10.1590/1984-3143-AR2023-0073
Jonas Gomes Flores, Verônica La Cruz Bueno, Henrique Boll de Araujo Bastos, Sandra Mara da Encarnação Fiala Rechsteiner
{"title":"Foal sex in Thoroughbred horses: related factors.","authors":"Jonas Gomes Flores, Verônica La Cruz Bueno, Henrique Boll de Araujo Bastos, Sandra Mara da Encarnação Fiala Rechsteiner","doi":"10.1590/1984-3143-AR2023-0073","DOIUrl":"10.1590/1984-3143-AR2023-0073","url":null,"abstract":"<p><p>Reproductive biotechniques in the equine species have advanced in the last decade and horse breeders have started to question the possibilities of interfering in the determination of foal sex. The aim of the present study was to verify whether the variables mares and stallion's age, side of the ovary containing the preovulatory follicle, preovulatory follicle diameter, time between breeding and ovulation, and ovulation inducing hormones influence the sex of the foal. A total of 259 reproductive cycles of 160 mares and 22 Thoroughbred stallions were used. Statistical analysis was performed using R software, including Pearson's chi-square test and logistic regression. Of the total foals born, 136 were males (52.51%) and 123 were females (47.49%). In mares that ovulated with -24h after ovulation induction, 104 foals (54.74%) were males and 86 (45.26%) were females, while in mares that ovulated with +24h, 32 foals (46.38%) were males and 37 (53.62%) were females. Stallions up to 15 years old had 44.14% (n=49) females and those over 15 years had 49.66% (n=73) females. The simple logistic regression model showed that mares and stallions under 15 years old, mares with ovulation time less than 24 hours and treated with Deslorelin had a higher probability of having male foals, but the Pearson's chi-square test showed that foals gender were not influenced by the variables studied.</p>","PeriodicalId":7889,"journal":{"name":"Animal Reproduction","volume":"21 3","pages":"e20230073"},"PeriodicalIF":1.6,"publicationDate":"2024-08-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11340788/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142034956","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Animal ReproductionPub Date : 2024-07-15eCollection Date: 2024-01-01DOI: 10.1590/1984-3143-AR2024-0012
Alesandro Silva Ferreira, Francisco Glauber Peixoto Ferreira, Etho Roberio Medeiros Nascimento, Gildas Mbemya Tetaping, Laritza Ferreira de Lima, Said Gonçalves da Cruz Fonseca, José Ricardo de Figueiredo, Daniel Freire de Sousa, Juliana Jales de Hollanda Celestino
{"title":"Evaluation of the morphology and development of preantral ovarian follicles in mice submitted to a chronic diet of dietary supplementation with <i>Pereskia aculeata Miller</i> leaves.","authors":"Alesandro Silva Ferreira, Francisco Glauber Peixoto Ferreira, Etho Roberio Medeiros Nascimento, Gildas Mbemya Tetaping, Laritza Ferreira de Lima, Said Gonçalves da Cruz Fonseca, José Ricardo de Figueiredo, Daniel Freire de Sousa, Juliana Jales de Hollanda Celestino","doi":"10.1590/1984-3143-AR2024-0012","DOIUrl":"10.1590/1984-3143-AR2024-0012","url":null,"abstract":"<p><p>This study aimed to investigate the effect of including mouse feed with different concentrations (5, 10, or 20%) of <i>Pereskia aculeata Miller</i> (PAM) leaves on the morphology and development of preantral ovarian follicles and ovarian stromal cell density. The oral toxicity was performed using repeated dose toxicity assays subdivided into experiments of 30 days and 90 days of treatment. After the experiments, the ovaries of each animal were collected and submitted to classical histology. At 30 and 90 days, there was an equivalent percentage of normal, primordial, and developing follicles (P > 0.05) between PAM treatments compared to the control. Regarding the different stages of follicular development, after 90 days, there was a higher percentage (P < 0.05) of developing follicles only in the control group compared to day 30. The PAM 5% treatment was the only one that affected the cell density in the stroma after 90 days of treatment. Thus, we observed that supplementing the diet with <i>P. aculeata</i> did not pose any risk concerning animal consumption; specifically, there were no toxic reproductive effects observed from adding <i>Pereskia aculeata Miller</i> to the mouse diet.</p>","PeriodicalId":7889,"journal":{"name":"Animal Reproduction","volume":"21 2","pages":"e20240012"},"PeriodicalIF":1.6,"publicationDate":"2024-07-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11253780/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141632426","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Animal ReproductionPub Date : 2024-07-15eCollection Date: 2024-01-01DOI: 10.1590/1984-3143-AR2023-0118
Monike Willemin Quirino, Carolini Schultz, Michele Dos Passos Dezordi Franz, Thomaz Lucia, Arthur Martelli, Paulo Bayard Dias Gonçalves, Rafael da Rosa Ulguim, Bernardo Garziera Gasperin, Ivan Bianchi
{"title":"Use of chorionic gonadotropins during lactation to optimize postpartum sow reproductive performance: a review.","authors":"Monike Willemin Quirino, Carolini Schultz, Michele Dos Passos Dezordi Franz, Thomaz Lucia, Arthur Martelli, Paulo Bayard Dias Gonçalves, Rafael da Rosa Ulguim, Bernardo Garziera Gasperin, Ivan Bianchi","doi":"10.1590/1984-3143-AR2023-0118","DOIUrl":"10.1590/1984-3143-AR2023-0118","url":null,"abstract":"<p><p>Treating lactating sows with chorionic gonadotropins may allow controlling their post-weaning reproductive function, despite the occurrence of anestrous during lactation. This article reviews the potential effectiveness of treatment with both equine and human chorionic gonadotropins (eCG and hCG, respectively) during lactation on the control of estrus expression and ovulation in weaned sows. The use of 1,000 IU hCG at 24 and 48 h postpartum may induce ovulation in the treated sows, but the ovulation rate may be variable. Pregnancy rates may be improved with combined treatment after the second week of lactation with both chorionic gonadotropins: 1,500 IU eCG plus 500 - 1,000 hCG; or 1,000 IU eCG plus 1,000 IU hCG. Treatment with eCG (1,000 - 2,000 IU) at the end of lactation may result in acceptable estrus expression and ovulation rates, although with marginal benefit for pregnancy rates. The subsequent response to treatments with chorionic gonadotropins during lactation is likely influenced by the treatment period, the suckling frequency during lactation, and the boar exposure during the weaning-to-estrus interval. A better understanding of the efficiency of such steroid-free treatments is increasingly relevant due to the constraints of the use of steroid hormones in livestock reproductive management.</p>","PeriodicalId":7889,"journal":{"name":"Animal Reproduction","volume":"21 2","pages":"e20230118"},"PeriodicalIF":1.6,"publicationDate":"2024-07-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11253785/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141632375","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Effect of HDAC9 on the differentiation of chicken embryonic stem cells into male germ cells.","authors":"Xin Li, Yongsheng Yu, Qi Zhang, Xiaotong Luo, Li Yu, Zhongli Zhao","doi":"10.1590/1984-3143-AR2024-0011","DOIUrl":"10.1590/1984-3143-AR2024-0011","url":null,"abstract":"<p><p>Histone deacetylase 9 (HDAC9) is a histone deacetylase (HDAC) subtype IIa protein that deacetylates histone 3 (H3), histone 4 (H4), and nonhistone proteins in vivo to alter chromosomal shape and regulate gene transcription. There have been few studies on the regulatory influence of the <i>HDAC</i>9 gene on the differentiation of chicken embryonic stem cells (cESCs) into male germ cells, and the significance of <i>HDAC</i>9 is still unknown. Therefore, we explored the specific role of <i>HDAC</i>9 during differentiation of the cESCs of Jilin Luhua chickens through inhibition or overexpression. In medium supplemented with 10<sup>-5</sup> mol/L retinoic acid (RA), cESCs were stimulated to develop into germ cells. HDAC9 and germline marker gene mRNA and protein levels were measured using qRT‒PCR and western blotting. During the differentiation of cESCs into male germ cells, overexpression of the HDAC9 gene greatly increased the mRNA and protein expression levels of the germline marker genes Stra8, Dazl, c-kit, and integrin ɑ6. The HDAC9 inhibitor TMP195 significantly decreased the mRNA and protein expression levels of the above markers. In summary, HDAC9 positively regulates the differentiation of cESCs.</p>","PeriodicalId":7889,"journal":{"name":"Animal Reproduction","volume":"21 2","pages":"e20240011"},"PeriodicalIF":1.6,"publicationDate":"2024-07-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11253783/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141632424","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}